BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00014887
         (540 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PNX96760.1| B3 domain-containing transcription factor vrn1-li...   117   2e-29
gb|PNX57160.1| B3 domain-containing transcription factor vrn1-li...   110   9e-28
gb|PNY09843.1| B3 domain-containing transcription factor vrn1-li...   110   3e-25
gb|PNX83304.1| B3 domain-containing transcription factor vrn1-li...   104   6e-25
dbj|GAU49059.1| hypothetical protein TSUD_25170 [Trifolium subte...   104   4e-23
ref|XP_013452436.1| B3 DNA-binding domain protein [Medicago trun...    94   4e-19
ref|XP_013466761.1| B3 DNA-binding domain protein [Medicago trun...    87   5e-17
ref|XP_013466758.1| B3 DNA-binding domain protein [Medicago trun...    87   7e-17
ref|XP_013466766.1| B3 DNA-binding domain protein [Medicago trun...    79   7e-14
gb|PNX78523.1| B3 domain-containing transcription factor vrn1-li...    75   7e-13
dbj|BAT93691.1| hypothetical protein VIGAN_08021700 [Vigna angul...    72   1e-11
ref|XP_017413234.1| PREDICTED: B3 domain-containing transcriptio...    72   2e-11
gb|KOM36395.1| hypothetical protein LR48_Vigan02g254500 [Vigna a...    72   3e-11
ref|XP_013466763.1| B3 DNA-binding domain protein [Medicago trun...    71   5e-11
ref|XP_019423704.1| PREDICTED: B3 domain-containing transcriptio...    69   2e-10
ref|XP_019423703.1| PREDICTED: B3 domain-containing transcriptio...    69   2e-10
dbj|BAT93692.1| hypothetical protein VIGAN_08021800 [Vigna angul...    68   4e-10
ref|XP_017414132.1| PREDICTED: B3 domain-containing protein Os03...    68   4e-10
ref|XP_014490873.1| B3 domain-containing transcription factor VR...    67   1e-09
ref|XP_004498233.1| PREDICTED: B3 domain-containing transcriptio...    66   2e-09

>gb|PNX96760.1| B3 domain-containing transcription factor vrn1-like protein
           [Trifolium pratense]
          Length = 220

 Score =  117 bits (292), Expect = 2e-29
 Identities = 60/110 (54%), Positives = 82/110 (74%), Gaps = 4/110 (3%)
 Frame = +1

Query: 1   RSCKRTNDSGRPDMKLNTFQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYKVEV 180
           RSC +T+D   P++  N F+++V +L+K+ IP+EFLK+H I NGN VELKVG  ++ VEV
Sbjct: 112 RSCPKTHDYESPNLH-NKFKVSVTTLDKLIIPSEFLKRHNISNGNSVELKVGEGTWFVEV 170

Query: 181 SYNQ--RSWRFSKGWKEFVKGCSLKVGDTCVFEMID--DEIIKVSIVLDV 318
           SYN+      F+KGWKEFV+ C +K+GDTC FEMID  + + KVSI+LDV
Sbjct: 171 SYNEDLDYGGFTKGWKEFVRECKVKIGDTCFFEMIDLQNHVFKVSIILDV 220


>gb|PNX57160.1| B3 domain-containing transcription factor vrn1-like protein
           [Trifolium pratense]
          Length = 124

 Score =  110 bits (274), Expect = 9e-28
 Identities = 54/92 (58%), Positives = 74/92 (80%), Gaps = 4/92 (4%)
 Frame = +1

Query: 55  FQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYKVEVSYNQR--SWRFSKGWKEF 228
           F+++V SL+KV+IP+EFLK+H I +GNLVELKVG  ++ VEV+YNQ      F+KGW+EF
Sbjct: 33  FEVSVTSLDKVAIPSEFLKRHNIFSGNLVELKVGEGTWFVEVNYNQHLDYGSFTKGWEEF 92

Query: 229 VKGCSLKVGDTCVFEMID--DEIIKVSIVLDV 318
           V+ C +K+GDTC+FEMID  + + KVSI+LDV
Sbjct: 93  VRECKVKIGDTCLFEMIDVQNHVFKVSIILDV 124


>gb|PNY09843.1| B3 domain-containing transcription factor vrn1-like protein
           [Trifolium pratense]
          Length = 394

 Score =  110 bits (274), Expect = 3e-25
 Identities = 60/111 (54%), Positives = 80/111 (72%), Gaps = 5/111 (4%)
 Frame = +1

Query: 1   RSCKRTNDSGRPDMKLNTFQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYKVEV 180
           RSC   +DS  P++  N F++ V SL+K+ IP+E LK+H I NGN VELKVG  ++ VEV
Sbjct: 287 RSCPN-HDSESPNLH-NKFEVFVTSLDKLVIPSELLKRHNISNGNSVELKVGEGTWFVEV 344

Query: 181 SYNQ---RSWRFSKGWKEFVKGCSLKVGDTCVFEMID--DEIIKVSIVLDV 318
           SYNQ     W F++GWKEFV+ C +K+GDTC+FEMID  + + K SI+LDV
Sbjct: 345 SYNQYLDYGW-FTEGWKEFVRECKVKIGDTCLFEMIDVQNHVFKFSIILDV 394


>gb|PNX83304.1| B3 domain-containing transcription factor vrn1-like protein
           [Trifolium pratense]
          Length = 185

 Score =  104 bits (260), Expect = 6e-25
 Identities = 54/104 (51%), Positives = 76/104 (73%), Gaps = 5/104 (4%)
 Frame = +1

Query: 22  DSGRPDMKLNTFQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYKVEVSYNQR-- 195
           DS  P++  NTF++ V SL  + +P+EFLKKH I +GN VELKVG  ++ VEVS+NQ   
Sbjct: 84  DSESPNLH-NTFKVFVTSLFMLVVPSEFLKKHNISSGNSVELKVGEGTWFVEVSFNQHLD 142

Query: 196 -SWRFSKGWKEFVKGCSLKVGDTCVFEMID--DEIIKVSIVLDV 318
             W F+KGW EFV+ C +K+GDTC+FE++D  + + KVS++LDV
Sbjct: 143 YGW-FTKGWPEFVRECKVKIGDTCLFEIVDLENHVFKVSVILDV 185


>dbj|GAU49059.1| hypothetical protein TSUD_25170 [Trifolium subterraneum]
          Length = 396

 Score =  104 bits (259), Expect = 4e-23
 Identities = 56/113 (49%), Positives = 77/113 (68%), Gaps = 7/113 (6%)
 Frame = +1

Query: 1   RSCKRTNDSGRPDMKLN--TFQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYKV 174
           R C +T+        ++   F+++V SL+KV+IP+EFLK+  I  GN VEL+VG   + V
Sbjct: 285 RGCPKTHGHWNSPCPISHYKFEVSVTSLDKVAIPSEFLKRQNILGGNSVELRVGEGKWFV 344

Query: 175 EVSYNQR---SWRFSKGWKEFVKGCSLKVGDTCVFEMID--DEIIKVSIVLDV 318
           EVSYNQ     W F+KGW+EFV+ C +K GDTC+FEMID  + + KVSI+LDV
Sbjct: 345 EVSYNQHLDYGW-FTKGWEEFVRECKVKSGDTCLFEMIDVQNHVFKVSIILDV 396


>ref|XP_013452436.1| B3 DNA-binding domain protein [Medicago truncatula]
 gb|KEH26464.1| B3 DNA-binding domain protein [Medicago truncatula]
          Length = 473

 Score = 94.0 bits (232), Expect = 4e-19
 Identities = 53/114 (46%), Positives = 78/114 (68%), Gaps = 8/114 (7%)
 Frame = +1

Query: 1   RSCKRTN---DSGRPDMKLNTFQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYK 171
           +SC +T+   +S  PD     F++ V SL+K+ +    LK+H I +G+ VELKVG  ++ 
Sbjct: 362 KSCTKTHGLRNSVSPDSH-EKFKVFVTSLDKLIVLPSLLKRHNIFSGDSVELKVGEGTWF 420

Query: 172 VEVSYNQR---SWRFSKGWKEFVKGCSLKVGDTCVFEMID--DEIIKVSIVLDV 318
           VEVSYNQ     W F+KGW EFV+ C++K+GDTC+FE+ID  + + +VSI+LDV
Sbjct: 421 VEVSYNQYLDYGW-FTKGWPEFVRECNVKIGDTCLFELIDVQNHVFEVSIILDV 473


>ref|XP_013466761.1| B3 DNA-binding domain protein [Medicago truncatula]
 gb|KEH40802.1| B3 DNA-binding domain protein [Medicago truncatula]
          Length = 383

 Score = 87.4 bits (215), Expect = 5e-17
 Identities = 52/112 (46%), Positives = 74/112 (66%), Gaps = 9/112 (8%)
 Frame = +1

Query: 1   RSCKRTNDSGRPDMKLNT---FQITVESLEK-VSIPTEFLKKHKIRNGNLVELKVGGKSY 168
           +SC +T    R  + LN    F++ + S +K V +P+E LKKH I +GN VEL+VG  ++
Sbjct: 274 KSCTKTYGF-RDSVSLNLHEMFKVFITSSDKLVVLPSELLKKHGIFSGNSVELRVGEGTW 332

Query: 169 KVEVSYNQR---SWRFSKGWKEFVKGCSLKVGDTCVFEMID--DEIIKVSIV 309
            V+VSYN+     W F+KGW+EFV+ C +K GDTC+FE ID  + + KVSIV
Sbjct: 333 FVDVSYNEHLDHGW-FTKGWQEFVRDCKVKTGDTCLFETIDVQNHVFKVSIV 383


>ref|XP_013466758.1| B3 DNA-binding domain protein [Medicago truncatula]
 gb|KEH40799.1| B3 DNA-binding domain protein [Medicago truncatula]
          Length = 332

 Score = 86.7 bits (213), Expect = 7e-17
 Identities = 44/100 (44%), Positives = 70/100 (70%), Gaps = 4/100 (4%)
 Frame = +1

Query: 22  DSGRPDMKLNTFQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYKVEVSYNQ--R 195
           D GR  MK NT ++ V++ +  S+P EF+KK    N N+VELK+G KS+ V+V+Y +   
Sbjct: 232 DEGRDVMKHNTLKVLVKN-KAPSVPKEFMKKGS--NENIVELKMGRKSWFVKVNYYESVH 288

Query: 196 SWRFSKGWKEFVKGCSLKVGDTCVFEMIDDE--IIKVSIV 309
            WRF+KGW++F++ C +++GDTC+F +ID+   +  VSI+
Sbjct: 289 GWRFNKGWRKFMEECKVEIGDTCLFNLIDERKFVFDVSII 328


>ref|XP_013466766.1| B3 DNA-binding domain protein [Medicago truncatula]
 gb|KEH40807.1| B3 DNA-binding domain protein [Medicago truncatula]
          Length = 414

 Score = 79.0 bits (193), Expect = 7e-14
 Identities = 46/106 (43%), Positives = 64/106 (60%), Gaps = 4/106 (3%)
 Frame = +1

Query: 1   RSCK--RTNDSGRPDMKLNTFQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYKV 174
           RSC    T+  G   M+ NT +I V S     I  EF K+HK  +G  V+   GGKS+ V
Sbjct: 304 RSCHVLPTDLEGGIVMEPNTIEIRVNSTYPY-IQHEFFKRHKECHGKFVQFTNGGKSWPV 362

Query: 175 EVSYNQRSWRFSKGWKEFVKGCSLKVGDTCVFEMIDDE--IIKVSI 306
           +V Y +R+ RF  GW++F + C LK+GDTC  ++IDD+  + KVSI
Sbjct: 363 KVQYYERTCRFYAGWRKFKEDCELKIGDTCKLKLIDDKKFVFKVSI 408


>gb|PNX78523.1| B3 domain-containing transcription factor vrn1-like protein
           [Trifolium pratense]
          Length = 286

 Score = 75.1 bits (183), Expect = 7e-13
 Identities = 38/90 (42%), Positives = 54/90 (60%)
 Frame = +1

Query: 19  NDSGRPDMKLNTFQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYKVEVSYNQRS 198
           +  GR  MK +TF++TV S     +P  FL +HK   G  VELKV  KS+ V+V Y    
Sbjct: 186 DSKGRNVMKHDTFELTVNSSLYPYVPNAFLHRHKECWGKFVELKVDEKSWSVKVKYYPGG 245

Query: 199 WRFSKGWKEFVKGCSLKVGDTCVFEMIDDE 288
            + S GW EF + C L++GD C F++ID++
Sbjct: 246 SKLSVGWTEFKEECKLEIGDRCRFKLIDEK 275


>dbj|BAT93691.1| hypothetical protein VIGAN_08021700 [Vigna angularis var.
           angularis]
          Length = 347

 Score = 72.0 bits (175), Expect = 1e-11
 Identities = 42/111 (37%), Positives = 66/111 (59%), Gaps = 8/111 (7%)
 Frame = +1

Query: 4   SCKRTNDSGRPDMKL----NTFQITVE--SLEKVSIPTEFLKKHKIRNGNLVELKVGGKS 165
           S  R +D   P+ K     N F + ++      V++P +FLK H IR+ N V L+VG KS
Sbjct: 237 SSPRFSDHSIPECKERELENHFSVCIKPKDFNSVNLPRDFLKNHGIRHSNAVILEVGKKS 296

Query: 166 YKVEVSYNQRSWRFSKGWKEFVKGCSLKVGDTCVFEMIDDE--IIKVSIVL 312
           + V++    RS   + GW+EF++ C LK GD C+FE+ID +  + +V+IV+
Sbjct: 297 WNVKLDSYCRS---TSGWREFIRECQLKSGDICLFELIDKKKMVFEVTIVV 344


>ref|XP_017413234.1| PREDICTED: B3 domain-containing transcription factor VRN1-like
           [Vigna angularis]
          Length = 432

 Score = 72.0 bits (175), Expect = 2e-11
 Identities = 42/111 (37%), Positives = 66/111 (59%), Gaps = 8/111 (7%)
 Frame = +1

Query: 4   SCKRTNDSGRPDMKL----NTFQITVE--SLEKVSIPTEFLKKHKIRNGNLVELKVGGKS 165
           S  R +D   P+ K     N F + ++      V++P +FLK H IR+ N V L+VG KS
Sbjct: 322 SSPRFSDHSIPECKERELENHFSVCIKPKDFNSVNLPRDFLKNHGIRHSNAVILEVGKKS 381

Query: 166 YKVEVSYNQRSWRFSKGWKEFVKGCSLKVGDTCVFEMIDDE--IIKVSIVL 312
           + V++    RS   + GW+EF++ C LK GD C+FE+ID +  + +V+IV+
Sbjct: 382 WNVKLDSYCRS---TSGWREFIRECQLKSGDICLFELIDKKKMVFEVTIVV 429


>gb|KOM36395.1| hypothetical protein LR48_Vigan02g254500 [Vigna angularis]
          Length = 827

 Score = 71.6 bits (174), Expect = 3e-11
 Identities = 42/110 (38%), Positives = 65/110 (59%), Gaps = 8/110 (7%)
 Frame = +1

Query: 4   SCKRTNDSGRPDMKL----NTFQITVE--SLEKVSIPTEFLKKHKIRNGNLVELKVGGKS 165
           S  R +D   P+ K     N F + ++      V++P +FLK H IR+ N V L+VG KS
Sbjct: 322 SSPRFSDHSIPECKERELENHFSVCIKPKDFNSVNLPRDFLKNHGIRHSNAVILEVGKKS 381

Query: 166 YKVEVSYNQRSWRFSKGWKEFVKGCSLKVGDTCVFEMIDDE--IIKVSIV 309
           + V++    RS   + GW+EF++ C LK GD C+FE+ID +  + +V+IV
Sbjct: 382 WNVKLDSYCRS---TSGWREFIRECQLKSGDICLFELIDKKKMVFEVTIV 428


>ref|XP_013466763.1| B3 DNA-binding domain protein [Medicago truncatula]
 gb|KEH40804.1| B3 DNA-binding domain protein [Medicago truncatula]
          Length = 406

 Score = 70.9 bits (172), Expect = 5e-11
 Identities = 41/96 (42%), Positives = 66/96 (68%), Gaps = 5/96 (5%)
 Frame = +1

Query: 34  PD-MKLNTFQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYKVEVSYNQR--SWR 204
           PD MK  TF++ V++    S+P EF+K+    + N+VELK+G KS+ V+V+Y +     R
Sbjct: 301 PDVMKPYTFKVLVKNTAP-SVPKEFMKRGC--HENIVELKMGRKSWFVKVNYYESICGCR 357

Query: 205 FSKGWKEFVKGCSLKVGDTCVFEMIDDE--IIKVSI 306
           FSKGW++F+K C +++GDTC+ ++ID+   +  VSI
Sbjct: 358 FSKGWRKFMKECKVEIGDTCLLKLIDERKFVFDVSI 393


>ref|XP_019423704.1| PREDICTED: B3 domain-containing transcription factor VRN1-like
           isoform X2 [Lupinus angustifolius]
          Length = 428

 Score = 68.9 bits (167), Expect = 2e-10
 Identities = 36/107 (33%), Positives = 69/107 (64%), Gaps = 6/107 (5%)
 Frame = +1

Query: 4   SCKRTNDSGRPDMK---LNTFQITV-ESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYK 171
           SC +  ++ + +++   +N F I + +S    SIP  F++ +    G   +L+VG +++ 
Sbjct: 315 SCLKDPEAPKANIRVSSINHFTIHIKDSSYLTSIPMSFMRNYVTLGGKKAKLRVGKRTWD 374

Query: 172 VEVSYNQ-RSW-RFSKGWKEFVKGCSLKVGDTCVFEMIDDEIIKVSI 306
           V+V Y Q R++ RF+ GW++F K C+L VGD+C+FE+ID+E  ++++
Sbjct: 375 VKVQYYQTRTYARFTDGWRDFTKECNLNVGDSCLFEIIDEENFELNV 421


>ref|XP_019423703.1| PREDICTED: B3 domain-containing transcription factor VRN1-like
           isoform X1 [Lupinus angustifolius]
 gb|OIV93153.1| hypothetical protein TanjilG_20815 [Lupinus angustifolius]
          Length = 452

 Score = 68.9 bits (167), Expect = 2e-10
 Identities = 36/107 (33%), Positives = 69/107 (64%), Gaps = 6/107 (5%)
 Frame = +1

Query: 4   SCKRTNDSGRPDMK---LNTFQITV-ESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYK 171
           SC +  ++ + +++   +N F I + +S    SIP  F++ +    G   +L+VG +++ 
Sbjct: 339 SCLKDPEAPKANIRVSSINHFTIHIKDSSYLTSIPMSFMRNYVTLGGKKAKLRVGKRTWD 398

Query: 172 VEVSYNQ-RSW-RFSKGWKEFVKGCSLKVGDTCVFEMIDDEIIKVSI 306
           V+V Y Q R++ RF+ GW++F K C+L VGD+C+FE+ID+E  ++++
Sbjct: 399 VKVQYYQTRTYARFTDGWRDFTKECNLNVGDSCLFEIIDEENFELNV 445


>dbj|BAT93692.1| hypothetical protein VIGAN_08021800 [Vigna angularis var.
           angularis]
          Length = 432

 Score = 68.2 bits (165), Expect = 4e-10
 Identities = 42/110 (38%), Positives = 64/110 (58%), Gaps = 8/110 (7%)
 Frame = +1

Query: 4   SCKRTNDSGRPDMKL----NTFQITVE--SLEKVSIPTEFLKKHKIRNGNLVELKVGGKS 165
           S  R +D   P+ K     N F + ++   L  V++P +F K H IR+ N V L+VG KS
Sbjct: 322 SSPRFSDHSIPECKERELENHFSVCLKPKDLYYVNLPRDFFKNHGIRHSNAVILEVGKKS 381

Query: 166 YKVEVSYNQRSWRFSKGWKEFVKGCSLKVGDTCVFEMIDDE--IIKVSIV 309
           + V++       RF+ GW EF++GC LK GD C FE++D +  + +V+IV
Sbjct: 382 WDVKLD---PYCRFTVGWCEFIRGCQLKPGDVCRFELMDKKKIVFEVTIV 428


>ref|XP_017414132.1| PREDICTED: B3 domain-containing protein Os03g0620400-like [Vigna
           angularis]
 gb|KOM36394.1| hypothetical protein LR48_Vigan02g254400 [Vigna angularis]
          Length = 432

 Score = 68.2 bits (165), Expect = 4e-10
 Identities = 42/110 (38%), Positives = 64/110 (58%), Gaps = 8/110 (7%)
 Frame = +1

Query: 4   SCKRTNDSGRPDMKL----NTFQITVE--SLEKVSIPTEFLKKHKIRNGNLVELKVGGKS 165
           S  R +D   P+ K     N F + ++   L  V++P +F K H IR+ N V L+VG KS
Sbjct: 322 SSPRFSDHSIPECKERELENHFSVCLKPKDLYYVNLPRDFFKNHGIRHSNAVILEVGKKS 381

Query: 166 YKVEVSYNQRSWRFSKGWKEFVKGCSLKVGDTCVFEMIDDE--IIKVSIV 309
           + V++       RF+ GW EF++GC LK GD C FE++D +  + +V+IV
Sbjct: 382 WDVKLD---PYCRFTVGWCEFIRGCQLKPGDVCRFELMDKKKIVFEVTIV 428


>ref|XP_014490873.1| B3 domain-containing transcription factor VRN1-like [Vigna radiata
           var. radiata]
          Length = 390

 Score = 66.6 bits (161), Expect = 1e-09
 Identities = 29/69 (42%), Positives = 45/69 (65%), Gaps = 2/69 (2%)
 Frame = +1

Query: 88  SIPTEFLKKHKIRNGNLVELKVGGKSYKVEVSYNQRSW--RFSKGWKEFVKGCSLKVGDT 261
           +IP E+ KK    +G  V  +VG KS++V+  + ++S   RFS+GW +F + C LK+GD 
Sbjct: 305 TIPYEYAKKFAEEHGRFVTFRVGDKSWQVKFLFYEKSHYGRFSRGWHDFARDCDLKMGDA 364

Query: 262 CVFEMIDDE 288
           C+F M+D E
Sbjct: 365 CIFRMVDQE 373


>ref|XP_004498233.1| PREDICTED: B3 domain-containing transcription factor VRN1-like
           [Cicer arietinum]
          Length = 409

 Score = 66.2 bits (160), Expect = 2e-09
 Identities = 40/98 (40%), Positives = 57/98 (58%), Gaps = 2/98 (2%)
 Frame = +1

Query: 19  NDSGRPDMKLNTFQITVESLEKVSIPTEFLKKHKIRNGNLVELKVGGKSYKVEVSYNQRS 198
           N  G   MK NTF++ V S     +  EFLK     +G  ++LKV GKS+ V+VS   +S
Sbjct: 305 NLEGAIMMKQNTFKVYVNSSYPY-LSNEFLKTFSDCHGKFLKLKVKGKSWNVKVSNYPKS 363

Query: 199 WRFSKGWKEFVKGCSLKVGDTCVFEMIDDE--IIKVSI 306
            R   GW +F +G  L+ GD CV E+ID++  + +VSI
Sbjct: 364 GRLYAGWSQFKEGNKLEFGDICVLELIDEKKFVFEVSI 401