BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00010588
         (2649 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004511551.1| PREDICTED: E3 SUMO-protein ligase pli1 isofo...   982   0.0  
emb|CAA66482.1| transcription factor [Vicia faba var. minor]          934   0.0  
ref|XP_003611051.2| transcription factor-like protein [Medicago ...   872   0.0  
ref|XP_006573790.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...   867   0.0  
ref|XP_004511552.1| PREDICTED: E3 SUMO-protein ligase pli1 isofo...   860   0.0  
ref|XP_003538690.1| PREDICTED: uncharacterized protein LOC100784...   859   0.0  
ref|XP_019421531.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   858   0.0  
gb|KHN35048.1| E3 SUMO-protein ligase pli1 [Glycine soja]             853   0.0  
ref|XP_019421530.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   853   0.0  
ref|XP_006590503.1| PREDICTED: uncharacterized protein LOC100784...   844   0.0  
ref|XP_020216461.1| E4 SUMO-protein ligase PIAL2-like isoform X1...   844   0.0  
gb|OIV94923.1| hypothetical protein TanjilG_22120 [Lupinus angus...   845   0.0  
ref|XP_016202143.1| E4 SUMO-protein ligase PIAL2 [Arachis ipaensis]   825   0.0  
ref|XP_015964493.1| E4 SUMO-protein ligase PIAL2 [Arachis durane...   820   0.0  
ref|XP_020216462.1| E4 SUMO-protein ligase PIAL2-like isoform X2...   820   0.0  
gb|KRH27884.1| hypothetical protein GLYMA_11G020900 [Glycine max]     804   0.0  
ref|XP_017407252.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   787   0.0  
ref|XP_022643149.1| E4 SUMO-protein ligase PIAL2 isoform X2 [Vig...   785   0.0  
ref|XP_017407251.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isof...   785   0.0  
ref|XP_014521171.1| E4 SUMO-protein ligase PIAL2 isoform X1 [Vig...   780   0.0  

>ref|XP_004511551.1| PREDICTED: E3 SUMO-protein ligase pli1 isoform X1 [Cicer arietinum]
          Length = 834

 Score =  982 bits (2538), Expect = 0.0
 Identities = 523/775 (67%), Positives = 591/775 (76%), Gaps = 18/775 (2%)
 Frame = +3

Query: 363  MEMNNSPPLGMPPDMG----TGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFN 530
            ME+N SP L M PD+G    TG  +SPS VNLYRI  VVERL++HVQP  NRTD FEFFN
Sbjct: 17   MEVNTSPALPMHPDLGQLNGTGNPVSPSLVNLYRITKVVERLALHVQPG-NRTDSFEFFN 75

Query: 531  LCLSLSRGIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGW 710
            LCLSLSRGIDYALANGETP + NELP LMKQ+YQR  DE+S AAVMVLMISVKNACKIGW
Sbjct: 76   LCLSLSRGIDYALANGETPLKANELPTLMKQMYQRKTDELSLAAVMVLMISVKNACKIGW 135

Query: 711  FEMKESEELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAK 890
            F+ KESEELLTI+DE+GK+YCTLGN++TGPSS HS + TIM RFYPK+KLGPI+VSIEAK
Sbjct: 136  FQKKESEELLTISDEIGKIYCTLGNVSTGPSSCHSAMLTIMERFYPKLKLGPIIVSIEAK 195

Query: 891  PGYGASAIDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNV 1070
            PGYGA+A+DFHITKN  L DKKIWL VAQTDNIETSACLISPQ+VNFLLNGKGIDTRTN+
Sbjct: 196  PGYGAAAVDFHITKNNVLSDKKIWLLVAQTDNIETSACLISPQQVNFLLNGKGIDTRTNI 255

Query: 1071 RMDPGPQMPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVL-EDYVQPAI 1247
            RMDPGPQMPT+VTSMLK+GTNLLQAVGQFNG+YIILVAY+NA  LPEHPVL  DYVQPA+
Sbjct: 256  RMDPGPQMPTSVTSMLKFGTNLLQAVGQFNGNYIILVAYMNAAPLPEHPVLPPDYVQPAV 315

Query: 1248 TSVDSDIIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHC 1427
            TSVDSDIIEG SRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNF N+N KRPSWRCPHC
Sbjct: 316  TSVDSDIIEGASRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFTNINSKRPSWRCPHC 375

Query: 1428 NQSVCYTDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKE 1607
            NQ VCYTDIRLDR MIEILKNVGEN++EVI+HADGSWK VL+N  +V K+QNKA   EKE
Sbjct: 376  NQYVCYTDIRLDRKMIEILKNVGENVLEVIVHADGSWKAVLQNDHEVDKIQNKAAYREKE 435

Query: 1608 QTELRDSTCSPNSAPDVLDLTNDDNHLDIMDTCATADRKPSVPSQFVTPNSTSLSVNSTG 1787
            QTE +++TCSPN+ PDVLDLT +DN+LDIMDTC T DRKP     F    S+ + +    
Sbjct: 436  QTEQQETTCSPNTVPDVLDLT-EDNYLDIMDTCETTDRKP-----FQASVSSGVQIE--- 486

Query: 1788 VHQNVAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXX 1967
                     D++WAG Y+ NS  D PTVG+ +HPVL   VSP  NQEAEGH  IP     
Sbjct: 487  ---------DDFWAGFYMNNSGSDAPTVGI-DHPVLADAVSPPFNQEAEGHDIIPAINSA 536

Query: 1968 XXXXXXXXXXVQLLNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTI 2147
                      +QL+N MNS     ++G SS +PRHI RT V VQA PIQSQ  G QQ+++
Sbjct: 537  MHNQFFPSNNLQLMNYMNS---SNEYGSSSVSPRHIQRTPVAVQALPIQSQTLGSQQNSV 593

Query: 2148 TNLDSLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSS-TP 2312
            TNLDSL+ SS  ATPHV                D ERQQLFS++ LN  QVS    +  P
Sbjct: 594  TNLDSLITSSLSATPHVSLSNPASADSYNAILSDLERQQLFSQAPLNMSQVSAATQNRVP 653

Query: 2313 TSQHQSATQIRVPPVNMSAPTQ------LQSPYRAPARLNEFRNPHLQQALNPQQHQPMQ 2474
                 + TQ RVP VNMSAP Q      LQ+PYRA   LN+FRN HLQQ LNP+ HQPMQ
Sbjct: 654  PGNMSATTQNRVPSVNMSAPNQNRAPSHLQNPYRA-GMLNDFRNSHLQQTLNPRAHQPMQ 712

Query: 2475 TSNTHRSYIQQGGSQGRTLNGQQARVMASSQVSRQGE-RG-PVQSVSRTDELNNS 2633
             SNT  S++QQG       N QQARVMASS V+RQGE RG PVQ+VSRT+EL NS
Sbjct: 713  PSNTQWSHVQQGCPS----NNQQARVMASSHVARQGEQRGPPVQAVSRTNELFNS 763


>emb|CAA66482.1| transcription factor [Vicia faba var. minor]
          Length = 828

 Score =  934 bits (2413), Expect = 0.0
 Identities = 509/782 (65%), Positives = 579/782 (74%), Gaps = 25/782 (3%)
 Frame = +3

Query: 363  MEMNNSPPLGMPPDM----GTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFN 530
            ME N S    MPPD+    GT   +SPS VNLYRI  V++RL+ H QP  NR+D FEFFN
Sbjct: 1    MESNTSS--SMPPDLAATNGTTNPVSPSLVNLYRITKVLDRLAFHFQPG-NRSDSFEFFN 57

Query: 531  LCLSLSRGIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGW 710
            LCLSLSRGIDYALANGE PP+ NELP LMKQ+YQR  DE+S AAVMVLMISVKNACKIGW
Sbjct: 58   LCLSLSRGIDYALANGEPPPKANELPTLMKQMYQRKTDELSLAAVMVLMISVKNACKIGW 117

Query: 711  FEMKESEELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAK 890
            F+ KESEELLTIADE+GK+YCTLGN+  GPSS HS V TIM RFYP++KLGPI+V+IEA+
Sbjct: 118  FQKKESEELLTIADEIGKIYCTLGNIINGPSSSHSAVLTIMERFYPRMKLGPIIVTIEAQ 177

Query: 891  PGYGASAIDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNV 1070
            PGYGASA+DFHITKN    DKKIWL VAQTDNIETSACLISPQEVNFLLNGKGIDTRTN 
Sbjct: 178  PGYGASAVDFHITKNNVHSDKKIWLLVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNF 237

Query: 1071 RMDPGPQMPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVL-EDYVQPAI 1247
            RMDPGPQMPTNVTS+LK+GTNLLQAVGQFNGHYIILVAY++  SLPEHPVL  DYVQPA+
Sbjct: 238  RMDPGPQMPTNVTSVLKFGTNLLQAVGQFNGHYIILVAYMSVASLPEHPVLPPDYVQPAV 297

Query: 1248 TSV--DSDIIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCP 1421
            TSV  DSDIIEG SR SLNCPISFTRIKTPVKG SCKHFQCFDFDNFI +N KRPSWRCP
Sbjct: 298  TSVDSDSDIIEGASRFSLNCPISFTRIKTPVKGRSCKHFQCFDFDNFIKINSKRPSWRCP 357

Query: 1422 HCNQSVCYTDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCE 1601
            HCNQ+V YT+IRLDRNMIEIL+ VGENIVEV +HADGSW+ VLEN  DVGK+QNK  +C+
Sbjct: 358  HCNQNVSYTEIRLDRNMIEILEKVGENIVEVTVHADGSWQPVLENDHDVGKIQNKVHNCD 417

Query: 1602 KEQTELRDSTCSPNSAPDVLDLTNDDNHLD-IMDTCATADRKPSVPSQFVTPNSTSLSVN 1778
            KEQTE ++S  SP++ P V+DLTN DN +D IMDTC TADRK   PSQ   P S  +   
Sbjct: 418  KEQTEQQESARSPDTFPHVVDLTNKDNDMDVIMDTCETADRK---PSQGSAPTSVQIE-- 472

Query: 1779 STGVHQNVAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXX 1958
                        D++WAG+Y+AN+  DTPTVGV++  VL   VSPA+ QE+EGH +I   
Sbjct: 473  ------------DDFWAGLYIANTGSDTPTVGVTDLAVLADAVSPALIQESEGHDSISAN 520

Query: 1959 XXXXXXXXXXXXXVQLLNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQ 2138
                           + N M+S V EY  GRSSS+PRHI RT V VQA P+ SQ  G QQ
Sbjct: 521  HNQFLALNNLQ---MMNNYMSSFVSEY--GRSSSSPRHIQRTPVAVQALPVPSQPLGPQQ 575

Query: 2139 STITNLDSLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSS 2306
            +++TNLDS LI+SSP+  HV                DAERQQLFSRS LN PQVS    +
Sbjct: 576  NSVTNLDS-LITSSPSATHVSLSNPASADPYNAILSDAERQQLFSRSPLNMPQVSAATQN 634

Query: 2307 -TPTSQHQSATQIRVPPVNMS------APTQLQSPYRAPARLNEFRNPHLQQALNPQQHQ 2465
              P+    + T  RVPPV+MS      AP+ LQ+       LN+FRN HLQQ LNP+ H 
Sbjct: 635  RMPSVNMPAPTHNRVPPVSMSATTLNRAPSHLQNQQYRAGMLNDFRNSHLQQTLNPRAHT 694

Query: 2466 PMQTSNTHRSYIQQGGSQ----GRTLNGQQARVMASSQVSRQGE-RG-PVQSVSRTDELN 2627
            PMQ  N  RS+ QQG SQ    G   N QQARVMASS V+RQGE RG PVQ+VSRTDEL 
Sbjct: 695  PMQPLNAQRSHTQQGVSQTNAAGGAANSQQARVMASSHVARQGEQRGPPVQAVSRTDELF 754

Query: 2628 NS 2633
            NS
Sbjct: 755  NS 756


>ref|XP_003611051.2| transcription factor-like protein [Medicago truncatula]
 gb|AES94009.2| transcription factor-like protein [Medicago truncatula]
          Length = 819

 Score =  872 bits (2253), Expect = 0.0
 Identities = 478/780 (61%), Positives = 549/780 (70%), Gaps = 23/780 (2%)
 Frame = +3

Query: 363  MEMNNSPPLGMPPDMG----TGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFN 530
            ME N S PL   P+ G    T   +SPS VNLYRI  V+ERL+ H  P  NR+D FEFFN
Sbjct: 1    METNTSSPLSTLPESGAMTATANPVSPSLVNLYRITKVLERLATHFVPG-NRSDAFEFFN 59

Query: 531  LCLSLSRGIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGW 710
            LCLSLSRGIDYALANGE P + NELP+LMKQ+YQR  D+ SQAAVMVLMISVKNAC+IGW
Sbjct: 60   LCLSLSRGIDYALANGEVPLKANELPILMKQMYQRKTDDHSQAAVMVLMISVKNACEIGW 119

Query: 711  FEMKESEELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAK 890
            F   +S+ELL IAD++GK YCTLGN    P+S H  V TIM R+YP ++LGPI+VSIEAK
Sbjct: 120  FRENDSKELLNIADKIGKTYCTLGNAIAEPNSCHPAVLTIMQRYYPNMELGPIIVSIEAK 179

Query: 891  PGYGASAIDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNV 1070
            PGYGASA+DFHITKN    DKKIWL VAQ DNIE SACLISPQ+VN LLNGKGI TRTN 
Sbjct: 180  PGYGASAVDFHITKNNVHSDKKIWLLVAQIDNIEKSACLISPQQVNILLNGKGIHTRTNF 239

Query: 1071 RMDPGPQMPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVL-EDYVQPAI 1247
            RMDPGPQMPT VT +LK+GTNLLQAVGQF+GHYIILVAY++  SLP HPVL  DYVQPA+
Sbjct: 240  RMDPGPQMPTCVTGILKFGTNLLQAVGQFDGHYIILVAYMSVGSLPAHPVLPPDYVQPAV 299

Query: 1248 T-SVDSDIIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPH 1424
            +   DSDIIEG S+ISLNCPIS TRIKTPVKGHSCKHFQCFDFDNFI +NFKRPSWRCPH
Sbjct: 300  SVDADSDIIEGESKISLNCPISRTRIKTPVKGHSCKHFQCFDFDNFIKINFKRPSWRCPH 359

Query: 1425 CNQSVCYTDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEK 1604
            C + VCYTDIRLDRNMIE+L+ VGENIVEV   ADGSWK   EN  DV K+QNKA DCE 
Sbjct: 360  CTRPVCYTDIRLDRNMIEVLEKVGENIVEVTFEADGSWKVGSENDHDVNKIQNKAYDCEM 419

Query: 1605 EQTELRDSTCSPNSAPDVLDLTNDDNHLDIMDTCATADRKPSVPSQFVTPNSTSLSVNST 1784
            EQTE ++STC P++  +++DLTN+DN LDIM T  TADRKP     F     T + +   
Sbjct: 420  EQTEQQESTCPPDTVSNIVDLTNNDNDLDIMCTYETADRKP-----FQASAPTGVQIE-- 472

Query: 1785 GVHQNVAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXX 1964
                      +++WAG+Y+AN    TPT  V E P L   VSP  NQEAEGH N+P    
Sbjct: 473  ----------EDFWAGLYIANGGSGTPT-AVVEIPELADAVSPVFNQEAEGHDNVP---- 517

Query: 1965 XXXXXXXXXXXVQLLNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQST 2144
                       + L+N MNS     ++GRSSSA R I RT   +QA P+QSQ  G QQ+ 
Sbjct: 518  AMHNQFLGQSNLTLMNYMNS----NEYGRSSSAARLIHRTPTAIQALPVQSQTLGPQQNP 573

Query: 2145 ITNLDSLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLN-PPQVSGVGSST 2309
             TNLDSL+ S+  A PHV                DAERQQLFSRS+LN PP ++   +  
Sbjct: 574  ATNLDSLITSNPSAAPHVSLSNPASADPYNAILSDAERQQLFSRSALNVPPVLAATQNRV 633

Query: 2310 PTSQHQSATQIRVPPVNMS------APTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPM 2471
            PT    + T  RV PVNMS      AP+QL +P      LN+FRN HLQQ LN   HQPM
Sbjct: 634  PTINIAAPTHNRVLPVNMSAATHNRAPSQLHNPPYRTDMLNDFRNSHLQQTLNAHAHQPM 693

Query: 2472 QTSNTHRSYIQQGGSQ----GRTLNGQQARVMASSQVSRQGE-RG-PVQSVSRTDELNNS 2633
            Q SN  RS+IQQGGSQ    G T + QQARVMASS V+RQGE RG PVQ+VS TDEL NS
Sbjct: 694  QPSNAQRSHIQQGGSQAYAAGATASSQQARVMASSHVARQGEQRGPPVQAVSSTDELFNS 753


>ref|XP_006573790.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like isoform X2 [Glycine max]
 gb|KRH77589.1| hypothetical protein GLYMA_01G222500 [Glycine max]
          Length = 876

 Score =  867 bits (2241), Expect = 0.0
 Identities = 470/749 (62%), Positives = 554/749 (73%), Gaps = 11/749 (1%)
 Frame = +3

Query: 369  MNNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLS 548
            MNNS P             SPS VNL+RI  V +RLS   QP  NR +P+EF+NLCLSLS
Sbjct: 1    MNNSLP-------ALTSDTSPSVVNLFRINKVADRLSWIAQPG-NRGEPYEFYNLCLSLS 52

Query: 549  RGIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKES 728
            RGIDYALANGETPP+ +ELP+L+KQI Q  NDE SQAA+MVLMIS+KNAC+IGWF+ KES
Sbjct: 53   RGIDYALANGETPPKAHELPLLVKQICQLKNDECSQAAMMVLMISIKNACEIGWFQTKES 112

Query: 729  EELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGAS 908
            EEL+TIADE+ KVY +LG +N GP S  + ++TIM +FYPK KLGPIL SIEA+PGYGAS
Sbjct: 113  EELVTIADEIRKVYSSLGTINVGPRSCSTAISTIMQKFYPKFKLGPILASIEAQPGYGAS 172

Query: 909  AIDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGP 1088
             +DFHITK++ L DK I+L VAQTDNIETSACLI+PQ+VNFLLNGKG+  RTNV+MDPGP
Sbjct: 173  VVDFHITKSEVLKDK-IFLLVAQTDNIETSACLINPQQVNFLLNGKGVLNRTNVQMDPGP 231

Query: 1089 QMPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSVDSD- 1265
            Q+PTNVT MLK+GTNLLQAVGQFNG Y++LVAY++ T   E PVL+DY+QPA+TSVDSD 
Sbjct: 232  QVPTNVTGMLKFGTNLLQAVGQFNGRYVVLVAYMSFTPFLEDPVLQDYLQPAVTSVDSDS 291

Query: 1266 -IIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVC 1442
             IIEG S+ISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMN KRPSWRCPHC Q+VC
Sbjct: 292  DIIEGASQISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNSKRPSWRCPHCIQNVC 351

Query: 1443 YTDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELR 1622
            Y DIRLDRNM+E+LKNVGENI EVI+ A+GSWK VLE   DV KMQ KAR+CEKEQT+ +
Sbjct: 352  YADIRLDRNMVEVLKNVGENITEVIVLANGSWKAVLEKDHDVDKMQKKARNCEKEQTQPQ 411

Query: 1623 DSTCSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVH 1793
            +STC P +    +DLT DD+ LD + +C   +RKP   S+ SQFVTPNSTSL +NSTGV+
Sbjct: 412  ESTCPPGT----VDLTKDDDGLDTVGSCDIVERKPTPASIHSQFVTPNSTSLGMNSTGVN 467

Query: 1794 QNVAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXX 1973
            QNVA QID++W GV    SR DTPTVG SE PVLP TVSP  +QE+ GH N P       
Sbjct: 468  QNVATQIDDFWPGVCFVRSRSDTPTVGNSELPVLPDTVSPTFSQESAGHDNNPVVNSAMH 527

Query: 1974 XXXXXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTIT 2150
                    +Q+ +N MNSV    ++GRSSSAPRHI RT V VQA P+QSQA G QQ++IT
Sbjct: 528  NQFLGPNNLQMQMNHMNSV---NEYGRSSSAPRHIHRTPVAVQALPVQSQALGPQQNSIT 584

Query: 2151 NLD-SLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPT 2315
            NL+ SLL S+S ATPH+                D ERQQ FSR+ +N PQVSGV S  P 
Sbjct: 585  NLNSSLLPSNSSATPHISLSNPTSVDTLNAILSDTERQQHFSRTPMNLPQVSGVNS--PA 642

Query: 2316 SQHQSATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPMQTSNTHRS 2495
             QH +ATQ R P +N SAPTQ Q+ YRA A  +EFRN HLQQALN  +  P ++SN    
Sbjct: 643  FQHHTATQNRGPLINTSAPTQPQNQYRANA-FSEFRNLHLQQALN-LRPPPPRSSNAQWP 700

Query: 2496 YIQQGGSQGRTLNGQQARVMASSQVSRQG 2582
             IQQG  Q       QA    +S  + QG
Sbjct: 701  RIQQGVPQSGNF---QAAARGASVAAGQG 726


>ref|XP_004511552.1| PREDICTED: E3 SUMO-protein ligase pli1 isoform X2 [Cicer arietinum]
          Length = 728

 Score =  860 bits (2223), Expect = 0.0
 Identities = 457/684 (66%), Positives = 519/684 (75%), Gaps = 14/684 (2%)
 Frame = +3

Query: 624  IYQRNNDEISQAAVMVLMISVKNACKIGWFEMKESEELLTIADELGKVYCTLGNMNTGPS 803
            +YQR  DE+S AAVMVLMISVKNACKIGWF+ KESEELLTI+DE+GK+YCTLGN++TGPS
Sbjct: 1    MYQRKTDELSLAAVMVLMISVKNACKIGWFQKKESEELLTISDEIGKIYCTLGNVSTGPS 60

Query: 804  SYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGASAIDFHITKNKFLPDKKIWLFVAQTD 983
            S HS + TIM RFYPK+KLGPI+VSIEAKPGYGA+A+DFHITKN  L DKKIWL VAQTD
Sbjct: 61   SCHSAMLTIMERFYPKLKLGPIIVSIEAKPGYGAAAVDFHITKNNVLSDKKIWLLVAQTD 120

Query: 984  NIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGPQMPTNVTSMLKYGTNLLQAVGQFNG 1163
            NIETSACLISPQ+VNFLLNGKGIDTRTN+RMDPGPQMPT+VTSMLK+GTNLLQAVGQFNG
Sbjct: 121  NIETSACLISPQQVNFLLNGKGIDTRTNIRMDPGPQMPTSVTSMLKFGTNLLQAVGQFNG 180

Query: 1164 HYIILVAYINATSLPEHPVL-EDYVQPAITSVDSDIIEGPSRISLNCPISFTRIKTPVKG 1340
            +YIILVAY+NA  LPEHPVL  DYVQPA+TSVDSDIIEG SRISLNCPISFTRIKTPVKG
Sbjct: 181  NYIILVAYMNAAPLPEHPVLPPDYVQPAVTSVDSDIIEGASRISLNCPISFTRIKTPVKG 240

Query: 1341 HSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCYTDIRLDRNMIEILKNVGENIVEVII 1520
            HSCKHFQCFDFDNF N+N KRPSWRCPHCNQ VCYTDIRLDR MIEILKNVGEN++EVI+
Sbjct: 241  HSCKHFQCFDFDNFTNINSKRPSWRCPHCNQYVCYTDIRLDRKMIEILKNVGENVLEVIV 300

Query: 1521 HADGSWKTVLENAQDVGKMQNKARDCEKEQTELRDSTCSPNSAPDVLDLTNDDNHLDIMD 1700
            HADGSWK VL+N  +V K+QNKA   EKEQTE +++TCSPN+ PDVLDLT +DN+LDIMD
Sbjct: 301  HADGSWKAVLQNDHEVDKIQNKAAYREKEQTEQQETTCSPNTVPDVLDLT-EDNYLDIMD 359

Query: 1701 TCATADRKPSVPSQFVTPNSTSLSVNSTGVHQNVAAQIDNYWAGVYVANSRPDTPTVGVS 1880
            TC T DRKP     F    S+ + +             D++WAG Y+ NS  D PTVG+ 
Sbjct: 360  TCETTDRKP-----FQASVSSGVQIE------------DDFWAGFYMNNSGSDAPTVGI- 401

Query: 1881 EHPVLPGTVSPAINQEAEGHANIPXXXXXXXXXXXXXXXVQLLNLMNSVVDEYQHGRSSS 2060
            +HPVL   VSP  NQEAEGH  IP               +QL+N MNS     ++G SS 
Sbjct: 402  DHPVLADAVSPPFNQEAEGHDIIPAINSAMHNQFFPSNNLQLMNYMNS---SNEYGSSSV 458

Query: 2061 APRHIGRTSVGVQARPIQSQASGLQQSTITNLDSLLISSSPATPHV----XXXXXXXXXX 2228
            +PRHI RT V VQA PIQSQ  G QQ+++TNLDSL+ SS  ATPHV              
Sbjct: 459  SPRHIQRTPVAVQALPIQSQTLGSQQNSVTNLDSLITSSLSATPHVSLSNPASADSYNAI 518

Query: 2229 XXDAERQQLFSRSSLNPPQVSGVGSS-TPTSQHQSATQIRVPPVNMSAPTQ------LQS 2387
              D ERQQLFS++ LN  QVS    +  P     + TQ RVP VNMSAP Q      LQ+
Sbjct: 519  LSDLERQQLFSQAPLNMSQVSAATQNRVPPGNMSATTQNRVPSVNMSAPNQNRAPSHLQN 578

Query: 2388 PYRAPARLNEFRNPHLQQALNPQQHQPMQTSNTHRSYIQQGGSQGRTLNGQQARVMASSQ 2567
            PYRA   LN+FRN HLQQ LNP+ HQPMQ SNT  S++QQG       N QQARVMASS 
Sbjct: 579  PYRA-GMLNDFRNSHLQQTLNPRAHQPMQPSNTQWSHVQQGCPS----NNQQARVMASSH 633

Query: 2568 VSRQGE-RG-PVQSVSRTDELNNS 2633
            V+RQGE RG PVQ+VSRT+EL NS
Sbjct: 634  VARQGEQRGPPVQAVSRTNELFNS 657


>ref|XP_003538690.1| PREDICTED: uncharacterized protein LOC100784204 isoform X1 [Glycine
            max]
 gb|KRH27883.1| hypothetical protein GLYMA_11G020900 [Glycine max]
          Length = 876

 Score =  859 bits (2219), Expect = 0.0
 Identities = 461/748 (61%), Positives = 553/748 (73%), Gaps = 11/748 (1%)
 Frame = +3

Query: 372  NNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLSR 551
            N+SP L +P D       SPS VN++RI  V +RL+   QP  NR +P EF+NLCLSLSR
Sbjct: 3    NSSPALTLPSDT------SPSVVNMFRINKVADRLAWIAQPG-NRGEPHEFYNLCLSLSR 55

Query: 552  GIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKESE 731
            GIDYALANGETPP+ ++LP+L+KQI Q  NDE SQAA+MVL+IS+KNAC+IGWF+ KESE
Sbjct: 56   GIDYALANGETPPKAHDLPLLVKQICQLKNDECSQAAMMVLLISIKNACEIGWFQTKESE 115

Query: 732  ELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGASA 911
            EL++IADE+GKVY +LG +N  P S  + ++TIM +FYPK KLGPIL SIEA+PGYGASA
Sbjct: 116  ELVSIADEIGKVYSSLGTINVRPRSCSTVISTIMQKFYPKFKLGPILASIEAQPGYGASA 175

Query: 912  IDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGPQ 1091
            +DFHITK++ L DK I+L VAQTDNIET ACLISPQ+VNFLLNGKG+  RTNV+MDPG Q
Sbjct: 176  VDFHITKSEVLKDK-IFLLVAQTDNIETPACLISPQQVNFLLNGKGVLNRTNVQMDPGAQ 234

Query: 1092 MPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSVD--SD 1265
            +PTNVT MLK+GTNLLQAVGQFNG Y++LVAY++ T L E PVL+DY+QPA+TSVD  SD
Sbjct: 235  VPTNVTGMLKFGTNLLQAVGQFNGRYVVLVAYMSVTPLLEDPVLQDYLQPAVTSVDLDSD 294

Query: 1266 IIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCY 1445
            IIEG SRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFIN+N KRPSWRCP C Q+VCY
Sbjct: 295  IIEGASRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFININSKRPSWRCPRCIQNVCY 354

Query: 1446 TDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELRD 1625
             DIRLDRNM+EILKNVGENI EVI+ A+GSWK VLE   DV KMQ KA +CEKEQT+ ++
Sbjct: 355  ADIRLDRNMVEILKNVGENITEVIVFANGSWKAVLEKDHDVDKMQKKAPNCEKEQTQPQE 414

Query: 1626 STCSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVHQ 1796
            STC P++    +DLT DD+ LD + +C   +RKP   S+ S FV+PN TSL +NSTGV+Q
Sbjct: 415  STCPPST----VDLTKDDDGLDTVGSCDIVERKPPPASIHSHFVSPNLTSLGMNSTGVNQ 470

Query: 1797 NVAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXXX 1976
            NVAAQ D++W GVY+  S  DTPTVG SE PVLP TVSPA +QE+ G  N P        
Sbjct: 471  NVAAQTDDFWTGVYIGRSSSDTPTVGNSELPVLPDTVSPAFSQESAGRDNNPVVNSAMHN 530

Query: 1977 XXXXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTITN 2153
                   +Q+ +N MNSV    ++GRSSSAPRHI RT V VQA P+QSQA G Q+++ITN
Sbjct: 531  QFSGPSNLQMQMNHMNSV---NEYGRSSSAPRHIHRTPVAVQALPVQSQALGPQENSITN 587

Query: 2154 LD-SLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPTS 2318
            L+ SLL S+S A PH+                D ERQQ FSR+ +NPPQVSGV S  P  
Sbjct: 588  LNSSLLPSNSSAAPHISLSNPASVDTLNAILSDTERQQHFSRTPVNPPQVSGVNS--PAF 645

Query: 2319 QHQSATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPMQTSNTHRSY 2498
            QH +ATQ RVP +N S PTQ Q+ YRA    +EFRN HLQQALN +   P  +SNT  S+
Sbjct: 646  QHHTATQNRVPLINTSVPTQPQNQYRANV-FSEFRNSHLQQALN-RWPPPSTSSNTQWSH 703

Query: 2499 IQQGGSQGRTLNGQQARVMASSQVSRQG 2582
            IQQ   Q       Q      +  +RQG
Sbjct: 704  IQQSVPQSGNF---QVAARGGALAARQG 728


>ref|XP_019421531.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X2 [Lupinus
            angustifolius]
          Length = 954

 Score =  858 bits (2217), Expect = 0.0
 Identities = 466/778 (59%), Positives = 553/778 (71%), Gaps = 37/778 (4%)
 Frame = +3

Query: 408  GTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLSRGIDYALANGETP 587
            G G  +SPS VN YRI AV ERL+ H+Q   NR +P+E ++LCLSLSRGIDYALANGE P
Sbjct: 37   GAGNPVSPSLVNTYRINAVAERLASHIQQRGNRCEPYEIYHLCLSLSRGIDYALANGEIP 96

Query: 588  PQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKESEELLTIADELGKV 767
             +  ELP LM+QI QR NDE   AA MVLMISVKNAC+IGWF  KE++ELL  ADE+GK+
Sbjct: 97   DRAQELPALMQQICQRKNDEQVHAAAMVLMISVKNACEIGWFGSKEAQELLKCADEMGKI 156

Query: 768  YCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGASAIDFHITKNKFLP 947
            YC+LGN+NTG +S HS + TIM RFYP++KLG IL S+EAKPGYGA  +DFHITKN  + 
Sbjct: 157  YCSLGNVNTGVTSCHSALQTIMERFYPRMKLGRILASMEAKPGYGACPVDFHITKNT-VR 215

Query: 948  DKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGPQMPTNVTSMLKYG 1127
             +KIWL VAQTDNIETSACLISPQ+VNFLLNGKG+D RT V MDPGPQMPTNVT MLK+G
Sbjct: 216  KEKIWLLVAQTDNIETSACLISPQQVNFLLNGKGVDRRTTVLMDPGPQMPTNVTGMLKFG 275

Query: 1128 TNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSVDSDIIEGPSRISLNCPI 1307
            TNLLQAVGQFNG+Y+IL+AY++ TS  EHPVL+ YVQPA+TSVDSDIIEG S+ISLNCPI
Sbjct: 276  TNLLQAVGQFNGNYVILIAYMSVTSSVEHPVLQHYVQPAVTSVDSDIIEGHSQISLNCPI 335

Query: 1308 SFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCYTDIRLDRNMIEILK 1487
            SFTRIKTPVKG SCKHFQCFDFDNFIN++ ++PSWRCPHCNQSV Y DIRLDRNM+++LK
Sbjct: 336  SFTRIKTPVKGRSCKHFQCFDFDNFINISSRKPSWRCPHCNQSVNYADIRLDRNMVDVLK 395

Query: 1488 NVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELRDSTCSPNSAPDVLDL 1667
            +VG+++ EVI+HADGSWK VLEN  +  KMQNKA +CEKEQTE ++S CSPN   +VLDL
Sbjct: 396  DVGDSVHEVIVHADGSWKAVLENDHNKDKMQNKAHNCEKEQTEPQESMCSPNIVSNVLDL 455

Query: 1668 TNDDNHLD-IMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVHQNVAAQI-DNYWAG 1832
            T DD+ ++ +M+T  TADRKP   SV  Q VTPNSTSL +NSTGV+QNV  QI D++W+G
Sbjct: 456  TEDDDQMEMMMNTVGTADRKPFQTSVHGQVVTPNSTSLGMNSTGVNQNVFPQIEDDFWSG 515

Query: 1833 VYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXXXXXXXXXXVQLLN 2012
            +           V VSEHP LP TVSPA+N E +GH N                 +QL  
Sbjct: 516  L-----------VSVSEHPTLPDTVSPALNLEGDGHDNNLAVNSVMHNQLSSPNNLQLHL 564

Query: 2013 LMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQS-QASGLQQSTITNLDSLLISSSPAT 2189
              NSV +EY  GR  S P HI RT + VQA P+Q  QA   QQ++ T  + L+ SSS A+
Sbjct: 565  NYNSVANEY--GR--SIPSHISRTPIAVQALPVQQPQALRPQQNSRTYSNPLISSSSTAS 620

Query: 2190 PHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPTSQHQSATQIRVPPV 2357
            PHV                D ERQQ FSR  +NPPQVSGV SS    QH SATQ RVP  
Sbjct: 621  PHVPLSSPPTADSFNAILSDTERQQRFSRPPMNPPQVSGVNSS--ALQHHSATQNRVPHF 678

Query: 2358 NMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPMQTSNTHRSYIQQGGSQ------ 2519
            N  +PTQLQ+PYRA     EF N HLQQALNPQ   P + SN  R   QQG S+      
Sbjct: 679  NTPSPTQLQNPYRA-GMFGEFGNQHLQQALNPQSLNPTRPSNMQRFQSQQGVSRPGIVQA 737

Query: 2520 -GRTLNGQQARVMASSQVSRQ---------------GER-----GPVQSVSRTDELNN 2630
             G T N  QARV+A++Q +RQ               G++     GP +SVSRTD+L N
Sbjct: 738  SGATANSHQARVIAAAQAARQSPPISVPIDSFRGLTGDQRGNVGGPPRSVSRTDDLIN 795


>gb|KHN35048.1| E3 SUMO-protein ligase pli1 [Glycine soja]
          Length = 874

 Score =  853 bits (2203), Expect = 0.0
 Identities = 461/748 (61%), Positives = 551/748 (73%), Gaps = 11/748 (1%)
 Frame = +3

Query: 372  NNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLSR 551
            N+SP L +P D       SPS VN++RI  V +RL+   QP  NR +P EF+NLCLSLSR
Sbjct: 3    NSSPALTLPSDT------SPSVVNMFRINKVADRLAWIAQPG-NRGEPHEFYNLCLSLSR 55

Query: 552  GIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKESE 731
            GIDYALANGETPP+ ++LP+L+KQI Q  NDE SQAA+MVL+IS+KNAC+IGWF+ KESE
Sbjct: 56   GIDYALANGETPPKAHDLPLLVKQICQLKNDECSQAAMMVLLISIKNACEIGWFQTKESE 115

Query: 732  ELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGASA 911
            EL++IADE+GKVY +LG +N  P S  + ++TIM +FYPK KLGPIL SIEA+PGYGASA
Sbjct: 116  ELVSIADEIGKVYSSLGTINVRPRSCSTVISTIMQKFYPKFKLGPILASIEAQPGYGASA 175

Query: 912  IDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGPQ 1091
            +DFHITK++ L D KI+L VAQTDNIET ACLISPQ+VNFLLNGKG+  RTNV+MDPG Q
Sbjct: 176  VDFHITKSEVLKD-KIFLLVAQTDNIETPACLISPQQVNFLLNGKGVLNRTNVQMDPGAQ 234

Query: 1092 MPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSV--DSD 1265
            +PTNVT MLK+GTNLLQAVGQFNG Y++LVAY++ T L E PVL+DY+QPA+TSV  DSD
Sbjct: 235  VPTNVTGMLKFGTNLLQAVGQFNGRYVVLVAYMSVTPLQEDPVLQDYLQPAVTSVDLDSD 294

Query: 1266 IIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCY 1445
            IIEG SRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFIN+N KRPSWRCP C Q+VCY
Sbjct: 295  IIEGASRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFININSKRPSWRCPRCIQNVCY 354

Query: 1446 TDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELRD 1625
             DIRLDRNM  ILKNVGENI EVI+ A+GSWK VLE   DV KMQ KA +CEKEQT+ ++
Sbjct: 355  ADIRLDRNM--ILKNVGENITEVIVFANGSWKAVLEKDHDVDKMQKKAPNCEKEQTQPQE 412

Query: 1626 STCSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVHQ 1796
            STC P++    +DLT DD+ LD + +C   +RKP   S+ S FV+PN TSL +NSTGV+Q
Sbjct: 413  STCPPST----VDLTKDDDGLDTVGSCDIVERKPPPASIHSHFVSPNLTSLGMNSTGVNQ 468

Query: 1797 NVAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXXX 1976
            NVAAQ D++W GVY+  S  DTPTVG SE PVLP TVSPA +QE+ G  N P        
Sbjct: 469  NVAAQTDDFWTGVYIGRSSSDTPTVGNSELPVLPDTVSPAFSQESAGRDNNPVVNSAMHN 528

Query: 1977 XXXXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTITN 2153
                   +Q+ +N MNSV    ++GRSSSAPRHI RT V VQA P+QSQA G QQ++ITN
Sbjct: 529  QFSGPSNLQMQMNHMNSV---NEYGRSSSAPRHIHRTPVAVQALPVQSQALGPQQNSITN 585

Query: 2154 LD-SLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPTS 2318
            L+ SLL S+S A PH+                D ERQQ FSR+ +NPPQVSGV S  P  
Sbjct: 586  LNSSLLPSNSSAAPHISLSNPASVDTLNAILSDTERQQHFSRTPVNPPQVSGVNS--PAF 643

Query: 2319 QHQSATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPMQTSNTHRSY 2498
            QH +ATQ RVP +N S PTQ Q+ YRA    +EFRN HLQQALN +   P  +SNT  S+
Sbjct: 644  QHHTATQNRVPLINTSVPTQPQNQYRANV-FSEFRNSHLQQALN-RWPPPSTSSNTQWSH 701

Query: 2499 IQQGGSQGRTLNGQQARVMASSQVSRQG 2582
            IQQ   Q       Q      +  +RQG
Sbjct: 702  IQQSVPQSGNF---QVAARGGALAARQG 726


>ref|XP_019421530.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Lupinus
            angustifolius]
          Length = 956

 Score =  853 bits (2204), Expect = 0.0
 Identities = 466/780 (59%), Positives = 553/780 (70%), Gaps = 39/780 (5%)
 Frame = +3

Query: 408  GTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLSRGIDYALANGETP 587
            G G  +SPS VN YRI AV ERL+ H+Q   NR +P+E ++LCLSLSRGIDYALANGE P
Sbjct: 37   GAGNPVSPSLVNTYRINAVAERLASHIQQRGNRCEPYEIYHLCLSLSRGIDYALANGEIP 96

Query: 588  PQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKESEELLTIADELGKV 767
             +  ELP LM+QI QR NDE   AA MVLMISVKNAC+IGWF  KE++ELL  ADE+GK+
Sbjct: 97   DRAQELPALMQQICQRKNDEQVHAAAMVLMISVKNACEIGWFGSKEAQELLKCADEMGKI 156

Query: 768  YCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGASAIDFHITKNKFLP 947
            YC+LGN+NTG +S HS + TIM RFYP++KLG IL S+EAKPGYGA  +DFHITKN  + 
Sbjct: 157  YCSLGNVNTGVTSCHSALQTIMERFYPRMKLGRILASMEAKPGYGACPVDFHITKNT-VR 215

Query: 948  DKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGPQMPTNVTSMLKYG 1127
             +KIWL VAQTDNIETSACLISPQ+VNFLLNGKG+D RT V MDPGPQMPTNVT MLK+G
Sbjct: 216  KEKIWLLVAQTDNIETSACLISPQQVNFLLNGKGVDRRTTVLMDPGPQMPTNVTGMLKFG 275

Query: 1128 TNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSV--DSDIIEGPSRISLNC 1301
            TNLLQAVGQFNG+Y+IL+AY++ TS  EHPVL+ YVQPA+TSV  DSDIIEG S+ISLNC
Sbjct: 276  TNLLQAVGQFNGNYVILIAYMSVTSSVEHPVLQHYVQPAVTSVDSDSDIIEGHSQISLNC 335

Query: 1302 PISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCYTDIRLDRNMIEI 1481
            PISFTRIKTPVKG SCKHFQCFDFDNFIN++ ++PSWRCPHCNQSV Y DIRLDRNM+++
Sbjct: 336  PISFTRIKTPVKGRSCKHFQCFDFDNFINISSRKPSWRCPHCNQSVNYADIRLDRNMVDV 395

Query: 1482 LKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELRDSTCSPNSAPDVL 1661
            LK+VG+++ EVI+HADGSWK VLEN  +  KMQNKA +CEKEQTE ++S CSPN   +VL
Sbjct: 396  LKDVGDSVHEVIVHADGSWKAVLENDHNKDKMQNKAHNCEKEQTEPQESMCSPNIVSNVL 455

Query: 1662 DLTNDDNHLD-IMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVHQNVAAQI-DNYW 1826
            DLT DD+ ++ +M+T  TADRKP   SV  Q VTPNSTSL +NSTGV+QNV  QI D++W
Sbjct: 456  DLTEDDDQMEMMMNTVGTADRKPFQTSVHGQVVTPNSTSLGMNSTGVNQNVFPQIEDDFW 515

Query: 1827 AGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXXXXXXXXXXVQL 2006
            +G+           V VSEHP LP TVSPA+N E +GH N                 +QL
Sbjct: 516  SGL-----------VSVSEHPTLPDTVSPALNLEGDGHDNNLAVNSVMHNQLSSPNNLQL 564

Query: 2007 LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQS-QASGLQQSTITNLDSLLISSSP 2183
                NSV +EY  GR  S P HI RT + VQA P+Q  QA   QQ++ T  + L+ SSS 
Sbjct: 565  HLNYNSVANEY--GR--SIPSHISRTPIAVQALPVQQPQALRPQQNSRTYSNPLISSSST 620

Query: 2184 ATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPTSQHQSATQIRVP 2351
            A+PHV                D ERQQ FSR  +NPPQVSGV SS    QH SATQ RVP
Sbjct: 621  ASPHVPLSSPPTADSFNAILSDTERQQRFSRPPMNPPQVSGVNSS--ALQHHSATQNRVP 678

Query: 2352 PVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPMQTSNTHRSYIQQGGSQ---- 2519
              N  +PTQLQ+PYRA     EF N HLQQALNPQ   P + SN  R   QQG S+    
Sbjct: 679  HFNTPSPTQLQNPYRA-GMFGEFGNQHLQQALNPQSLNPTRPSNMQRFQSQQGVSRPGIV 737

Query: 2520 ---GRTLNGQQARVMASSQVSRQ---------------GER-----GPVQSVSRTDELNN 2630
               G T N  QARV+A++Q +RQ               G++     GP +SVSRTD+L N
Sbjct: 738  QASGATANSHQARVIAAAQAARQSPPISVPIDSFRGLTGDQRGNVGGPPRSVSRTDDLIN 797


>ref|XP_006590503.1| PREDICTED: uncharacterized protein LOC100784204 isoform X2 [Glycine
            max]
          Length = 870

 Score =  844 bits (2181), Expect = 0.0
 Identities = 457/748 (61%), Positives = 547/748 (73%), Gaps = 11/748 (1%)
 Frame = +3

Query: 372  NNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLSR 551
            N+SP L +P D       SPS VN++RI  V +RL+   QP  NR +P EF+NLCLSLSR
Sbjct: 3    NSSPALTLPSDT------SPSVVNMFRINKVADRLAWIAQPG-NRGEPHEFYNLCLSLSR 55

Query: 552  GIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKESE 731
            GIDYALANGETPP+ ++LP+L+KQI Q  NDE SQAA+MVL+IS+KNAC+IGWF+ KESE
Sbjct: 56   GIDYALANGETPPKAHDLPLLVKQICQLKNDECSQAAMMVLLISIKNACEIGWFQTKESE 115

Query: 732  ELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGASA 911
            EL+      GKVY +LG +N  P S  + ++TIM +FYPK KLGPIL SIEA+PGYGASA
Sbjct: 116  ELI------GKVYSSLGTINVRPRSCSTVISTIMQKFYPKFKLGPILASIEAQPGYGASA 169

Query: 912  IDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGPQ 1091
            +DFHITK++ L DK I+L VAQTDNIET ACLISPQ+VNFLLNGKG+  RTNV+MDPG Q
Sbjct: 170  VDFHITKSEVLKDK-IFLLVAQTDNIETPACLISPQQVNFLLNGKGVLNRTNVQMDPGAQ 228

Query: 1092 MPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSVD--SD 1265
            +PTNVT MLK+GTNLLQAVGQFNG Y++LVAY++ T L E PVL+DY+QPA+TSVD  SD
Sbjct: 229  VPTNVTGMLKFGTNLLQAVGQFNGRYVVLVAYMSVTPLLEDPVLQDYLQPAVTSVDLDSD 288

Query: 1266 IIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCY 1445
            IIEG SRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFIN+N KRPSWRCP C Q+VCY
Sbjct: 289  IIEGASRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFININSKRPSWRCPRCIQNVCY 348

Query: 1446 TDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELRD 1625
             DIRLDRNM+EILKNVGENI EVI+ A+GSWK VLE   DV KMQ KA +CEKEQT+ ++
Sbjct: 349  ADIRLDRNMVEILKNVGENITEVIVFANGSWKAVLEKDHDVDKMQKKAPNCEKEQTQPQE 408

Query: 1626 STCSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVHQ 1796
            STC P++    +DLT DD+ LD + +C   +RKP   S+ S FV+PN TSL +NSTGV+Q
Sbjct: 409  STCPPST----VDLTKDDDGLDTVGSCDIVERKPPPASIHSHFVSPNLTSLGMNSTGVNQ 464

Query: 1797 NVAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXXX 1976
            NVAAQ D++W GVY+  S  DTPTVG SE PVLP TVSPA +QE+ G  N P        
Sbjct: 465  NVAAQTDDFWTGVYIGRSSSDTPTVGNSELPVLPDTVSPAFSQESAGRDNNPVVNSAMHN 524

Query: 1977 XXXXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTITN 2153
                   +Q+ +N MNSV    ++GRSSSAPRHI RT V VQA P+QSQA G Q+++ITN
Sbjct: 525  QFSGPSNLQMQMNHMNSV---NEYGRSSSAPRHIHRTPVAVQALPVQSQALGPQENSITN 581

Query: 2154 LD-SLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPTS 2318
            L+ SLL S+S A PH+                D ERQQ FSR+ +NPPQVSGV S  P  
Sbjct: 582  LNSSLLPSNSSAAPHISLSNPASVDTLNAILSDTERQQHFSRTPVNPPQVSGVNS--PAF 639

Query: 2319 QHQSATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPMQTSNTHRSY 2498
            QH +ATQ RVP +N S PTQ Q+ YRA    +EFRN HLQQALN +   P  +SNT  S+
Sbjct: 640  QHHTATQNRVPLINTSVPTQPQNQYRANV-FSEFRNSHLQQALN-RWPPPSTSSNTQWSH 697

Query: 2499 IQQGGSQGRTLNGQQARVMASSQVSRQG 2582
            IQQ   Q       Q      +  +RQG
Sbjct: 698  IQQSVPQSGNF---QVAARGGALAARQG 722


>ref|XP_020216461.1| E4 SUMO-protein ligase PIAL2-like isoform X1 [Cajanus cajan]
          Length = 892

 Score =  844 bits (2181), Expect = 0.0
 Identities = 464/749 (61%), Positives = 545/749 (72%), Gaps = 13/749 (1%)
 Frame = +3

Query: 369  MNNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLS 548
            MN S  L + PD       SPS VN+YRI  V +RL+   Q  S    P+EF+NLCLSLS
Sbjct: 1    MNTSSALPLLPDT------SPSTVNMYRISKVADRLAWLAQTGSRGDHPYEFYNLCLSLS 54

Query: 549  RGIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKES 728
            RGIDYA+ANGE P + ++LP+L+KQI +  NDE+SQAA+MVLMISVK+AC+IGWF+ KES
Sbjct: 55   RGIDYAVANGENPAKAHDLPLLVKQICKLKNDELSQAAMMVLMISVKSACEIGWFQTKES 114

Query: 729  EELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGAS 908
            EEL+TIADE+GKVY + G +N GPSS  S + TIM +FYPKIKLGPIL SI+AKPGYGA 
Sbjct: 115  EELVTIADEIGKVYSSSGILNAGPSSCSSAIPTIMEKFYPKIKLGPILTSIDAKPGYGAF 174

Query: 909  AIDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGP 1088
            A+DFHITKNK L D KI+L VAQTDNIETS CLISPQ+VNFLLNGK +  RTNV+MDPGP
Sbjct: 175  AVDFHITKNKVLKD-KIYLLVAQTDNIETSTCLISPQQVNFLLNGKAVTNRTNVQMDPGP 233

Query: 1089 QMPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSV--DS 1262
            QMPTNVT MLK+GTNLLQAVGQFN HYI+LVAY++  SLPE PVL+DY+QP +TSV  DS
Sbjct: 234  QMPTNVTGMLKFGTNLLQAVGQFNSHYIVLVAYLSVASLPEDPVLQDYLQPTVTSVDSDS 293

Query: 1263 DIIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVC 1442
            DIIEG S+ISLNCPISFTRIKTPVKG SCKHFQCFDFDNFI+MN KRPSWRCPHCNQ VC
Sbjct: 294  DIIEGASQISLNCPISFTRIKTPVKGRSCKHFQCFDFDNFISMNSKRPSWRCPHCNQYVC 353

Query: 1443 YTDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELR 1622
            Y DIRLDR M+EILKNVGENI EVI+ ADGSWK VLE  +DV KMQNKA +CEKEQTE +
Sbjct: 354  YADIRLDRVMVEILKNVGENITEVIVLADGSWKAVLEKDRDVDKMQNKAPNCEKEQTEPQ 413

Query: 1623 DSTCSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVH 1793
            +STCSP++    +DLT DD+ L+IMD+C  ADRKP   SV SQFVTPNSTSL +NSTG++
Sbjct: 414  ESTCSPST----VDLTKDDDCLEIMDSCEIADRKPFQASVHSQFVTPNSTSLGMNSTGIN 469

Query: 1794 QNVAAQI-DNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXX 1970
            QNVAAQI D++W+G+YV          G SE PVLP TVSPA NQE  G  N P      
Sbjct: 470  QNVAAQIEDDFWSGIYV---------TGGSELPVLPDTVSPAFNQEPAGRDNNPVVNSAM 520

Query: 1971 XXXXXXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTI 2147
                     +Q+ LN MNSV    ++GRSSS PR I RT V VQA P+QSQA G QQ++I
Sbjct: 521  HNSFSATNNLQMQLNHMNSV---NEYGRSSSMPR-INRTPVAVQALPVQSQALGPQQNSI 576

Query: 2148 TNLDSLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPT 2315
            TNL     ++S ATPH+                D ERQQ FSRS  N PQVSGV S+   
Sbjct: 577  TNL-----NNSAATPHINLSNPASVDAISAILSDTERQQHFSRSPRNLPQVSGVNST--A 629

Query: 2316 SQHQSATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQ--QHQPMQTSNTH 2489
             QH SATQ RVP VN SA T LQ+PYR  +  +EF+NPHLQQALNP+  Q Q +      
Sbjct: 630  FQHHSATQNRVPLVNTSASTLLQNPYRGRS-FSEFKNPHLQQALNPRPPQQQALNPRPPQ 688

Query: 2490 RSYIQQGGSQGRTLNGQQARVMASSQVSR 2576
            +  +     Q ++LN +      SS  S+
Sbjct: 689  QQSLNSRPPQQQSLNSRPPPPTRSSNDSQ 717


>gb|OIV94923.1| hypothetical protein TanjilG_22120 [Lupinus angustifolius]
          Length = 945

 Score =  845 bits (2183), Expect = 0.0
 Identities = 466/792 (58%), Positives = 554/792 (69%), Gaps = 51/792 (6%)
 Frame = +3

Query: 408  GTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLSRGIDYALANGETP 587
            G G  +SPS VN YRI AV ERL+ H+Q   NR +P+E ++LCLSLSRGIDYALANGE P
Sbjct: 14   GAGNPVSPSLVNTYRINAVAERLASHIQQRGNRCEPYEIYHLCLSLSRGIDYALANGEIP 73

Query: 588  PQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKESEELLTIADELGKV 767
             +  ELP LM+QI QR NDE   AA MVLMISVKNAC+IGWF  KE++ELL  ADE+GK+
Sbjct: 74   DRAQELPALMQQICQRKNDEQVHAAAMVLMISVKNACEIGWFGSKEAQELLKCADEMGKI 133

Query: 768  YCTLGNMNTGPSSYHSTVTTIM------------ARFYPKIKLGPILVSIEAKPGYGASA 911
            YC+LGN+NTG +S HS + TIM            +RFYP++KLG IL S+EAKPGYGA  
Sbjct: 134  YCSLGNVNTGVTSCHSALQTIMESSNKIPICGFPSRFYPRMKLGRILASMEAKPGYGACP 193

Query: 912  IDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGPQ 1091
            +DFHITKN  +  +KIWL VAQTDNIETSACLISPQ+VNFLLNGKG+D RT V MDPGPQ
Sbjct: 194  VDFHITKNT-VRKEKIWLLVAQTDNIETSACLISPQQVNFLLNGKGVDRRTTVLMDPGPQ 252

Query: 1092 MPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSVDSD-- 1265
            MPTNVT MLK+GTNLLQAVGQFNG+Y+IL+AY++ TS  EHPVL+ YVQPA+TSVDSD  
Sbjct: 253  MPTNVTGMLKFGTNLLQAVGQFNGNYVILIAYMSVTSSVEHPVLQHYVQPAVTSVDSDSD 312

Query: 1266 IIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCY 1445
            IIEG S+ISLNCPISFTRIKTPVKG SCKHFQCFDFDNFIN++ ++PSWRCPHCNQSV Y
Sbjct: 313  IIEGHSQISLNCPISFTRIKTPVKGRSCKHFQCFDFDNFINISSRKPSWRCPHCNQSVNY 372

Query: 1446 TDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELRD 1625
             DIRLDRNM+++LK+VG+++ EVI+HADGSWK VLEN  +  KMQNKA +CEKEQTE ++
Sbjct: 373  ADIRLDRNMVDVLKDVGDSVHEVIVHADGSWKAVLENDHNKDKMQNKAHNCEKEQTEPQE 432

Query: 1626 STCSPNSAPDVLDLTNDDNHLD-IMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVH 1793
            S CSPN   +VLDLT DD+ ++ +M+T  TADRKP   SV  Q VTPNSTSL +NSTGV+
Sbjct: 433  SMCSPNIVSNVLDLTEDDDQMEMMMNTVGTADRKPFQTSVHGQVVTPNSTSLGMNSTGVN 492

Query: 1794 QNVAAQI-DNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXX 1970
            QNV  QI D++W+G+           V VSEHP LP TVSPA+N E +GH N        
Sbjct: 493  QNVFPQIEDDFWSGL-----------VSVSEHPTLPDTVSPALNLEGDGHDNNLAVNSVM 541

Query: 1971 XXXXXXXXXVQLLNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQS-QASGLQQSTI 2147
                     +QL    NSV +EY  GR  S P HI RT + VQA P+Q  QA   QQ++ 
Sbjct: 542  HNQLSSPNNLQLHLNYNSVANEY--GR--SIPSHISRTPIAVQALPVQQPQALRPQQNSR 597

Query: 2148 TNLDSLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPT 2315
            T  + L+ SSS A+PHV                D ERQQ FSR  +NPPQVSGV SS   
Sbjct: 598  TYSNPLISSSSTASPHVPLSSPPTADSFNAILSDTERQQRFSRPPMNPPQVSGVNSS--A 655

Query: 2316 SQHQSATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPMQTSNTHRS 2495
             QH SATQ RVP  N  +PTQLQ+PYRA     EF N HLQQALNPQ   P + SN  R 
Sbjct: 656  LQHHSATQNRVPHFNTPSPTQLQNPYRA-GMFGEFGNQHLQQALNPQSLNPTRPSNMQRF 714

Query: 2496 YIQQGGSQ-------GRTLNGQQARVMASSQVSRQ---------------GER-----GP 2594
              QQG S+       G T N  QARV+A++Q +RQ               G++     GP
Sbjct: 715  QSQQGVSRPGIVQASGATANSHQARVIAAAQAARQSPPISVPIDSFRGLTGDQRGNVGGP 774

Query: 2595 VQSVSRTDELNN 2630
             +SVSRTD+L N
Sbjct: 775  PRSVSRTDDLIN 786


>ref|XP_016202143.1| E4 SUMO-protein ligase PIAL2 [Arachis ipaensis]
          Length = 881

 Score =  825 bits (2130), Expect = 0.0
 Identities = 453/778 (58%), Positives = 544/778 (69%), Gaps = 27/778 (3%)
 Frame = +3

Query: 378  SPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQP-NSNRTDPFEFFNLCLSLSRG 554
            +PPL  P D     + SPSF+N  RI AV+ERL+ H+   N N+    +F+N C+SLSRG
Sbjct: 8    TPPL-QPLDTAVLARTSPSFINNLRITAVMERLAAHLHDGNHNQLGALDFYNQCISLSRG 66

Query: 555  IDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKESEE 734
            IDY+LANGE  P+  ELP LM+  +QR NDE+SQAA+MV+MISVKNAC++GWF+ KE EE
Sbjct: 67   IDYSLANGEISPKAKELPALMRLFHQRKNDELSQAALMVVMISVKNACELGWFQAKEKEE 126

Query: 735  LLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGASAI 914
            LL IADE+ K++C  G +N GPSS HST+T IM RFYPK+KLG ILVS EAKPGYGA  +
Sbjct: 127  LLAIADEVAKIFCNAGKINAGPSSSHSTITRIMERFYPKMKLGQILVSFEAKPGYGAYTL 186

Query: 915  DFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGPQM 1094
            DFHITK+  +  ++IWLFVAQTDN+ETSACLI+PQ+VNFLLNGKG+D RTNV MD  PQM
Sbjct: 187  DFHITKSN-VQHERIWLFVAQTDNVETSACLINPQQVNFLLNGKGVDRRTNVFMDTVPQM 245

Query: 1095 PTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSV--DSDI 1268
            PT V+ MLKYGTNLLQAVGQF GHY+++VAY++  SLPE PVL+DYVQPA+TSV  DSD+
Sbjct: 246  PTPVSGMLKYGTNLLQAVGQFTGHYVVIVAYMSFVSLPEDPVLQDYVQPAVTSVDTDSDL 305

Query: 1269 IEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCYT 1448
            IEG SRISLNCPISF RIKTPVKG SCKHFQCFDFDNFINMN +RP WRCPHCNQ V Y 
Sbjct: 306  IEGASRISLNCPISFARIKTPVKGCSCKHFQCFDFDNFINMNSRRPWWRCPHCNQHVSYE 365

Query: 1449 DIRLDRNMIEILKNVGENIVEVIIHADGSW-----------KTVLENAQDVGKMQNKARD 1595
            DIRLDRNM+EILK+VGENI+EVI+  DGSW           KTV E   +V K QNKA +
Sbjct: 366  DIRLDRNMVEILKDVGENILEVIVLGDGSWKAVFEKDHNVDKTVFEKDHNVDKTQNKAHN 425

Query: 1596 CEKEQTELRDSTCSPNS-APDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNST 1763
             EK+  EL++STCSPN+   ++ DLTNDD+ +D+MDT    D KP   S  +QFV  +ST
Sbjct: 426  GEKKPAELQESTCSPNNIISNIFDLTNDDDCMDLMDTIELEDTKPAPGSAETQFVNQSST 485

Query: 1764 SLSVNSTGVHQNVAAQIDN-YWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGH 1940
            SL +NST V QNVA+ ID+ +WAG+ +   R DTP++G+SE+PVLP +VSPA NQ +  H
Sbjct: 486  SLGLNSTDVDQNVASHIDDEFWAGLDLVFGRSDTPSLGISENPVLPDSVSPAFNQGSNSH 545

Query: 1941 ANIPXXXXXXXXXXXXXXXVQLLNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQ 2120
             N                 +QL+  MNS V+EY  GRS+S PRH+ RT V VQA    S 
Sbjct: 546  DNNSAVNSLVHNQVSTPTNLQLI-YMNSSVNEY--GRSASIPRHVNRTPVAVQALAAPSS 602

Query: 2121 ASGLQQSTITNLDSLLISSSPATPHV--XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSG 2294
             SG +QS    L+S L SSSPAT HV              D ERQQ FSRS +N PQ+SG
Sbjct: 603  ISGREQSLGNTLNSSLPSSSPATTHVPLSHTATAANPILTDTERQQHFSRSQMNLPQMSG 662

Query: 2295 VGSSTPTSQHQSATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPMQ 2474
            V S      H SATQ RVPP N  AP QL +  R    L E  NPHL Q+LN + H P+ 
Sbjct: 663  VNSL--GLPHHSATQNRVPPPNNPAPNQLPNSNRPWVGLGELSNPHLSQSLNSRAH-PVM 719

Query: 2475 TSNTHRSYIQQGG----SQGRTLNGQQARVMASSQVSRQGERGPV--QSVSRTDELNN 2630
             +N  RS+IQQGG    S G T N QQ R  A  QVSR  +RG V  QSVSR D+L N
Sbjct: 720  RTNIQRSHIQQGGSVAQSTGTTANSQQTRANAIGQVSRD-QRGSVTPQSVSRPDDLLN 776


>ref|XP_015964493.1| E4 SUMO-protein ligase PIAL2 [Arachis duranensis]
          Length = 881

 Score =  820 bits (2119), Expect = 0.0
 Identities = 451/778 (57%), Positives = 544/778 (69%), Gaps = 27/778 (3%)
 Frame = +3

Query: 378  SPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQP-NSNRTDPFEFFNLCLSLSRG 554
            +PPL  P D     + SPSF+N  RI AV+ERL+ H+   N N+    +F+N C+SLSRG
Sbjct: 8    TPPL-QPLDTAVLARTSPSFINNLRITAVMERLAAHLHDGNHNQLGALDFYNQCISLSRG 66

Query: 555  IDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKESEE 734
            IDY+LANGE  P+  ELP LM+  +QR NDE+SQAA+MV+MISVKNAC++GWF+ KE EE
Sbjct: 67   IDYSLANGEISPKAKELPALMRLFHQRKNDELSQAALMVVMISVKNACELGWFQAKEKEE 126

Query: 735  LLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGASAI 914
            LL IADE+ K++C  G ++ GPSS HST+T IM RFYPK+KLG ILVS EAKPGYGA  +
Sbjct: 127  LLAIADEVAKIFCNAGKISAGPSSSHSTITRIMERFYPKMKLGQILVSFEAKPGYGAYTL 186

Query: 915  DFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGPQM 1094
            DFHITK   +  ++IWLFVAQTDN+ETSACLI+PQ+VNFLLNGKG+D RTNV MD  PQM
Sbjct: 187  DFHITKRN-VQHERIWLFVAQTDNVETSACLINPQQVNFLLNGKGVDRRTNVFMDTVPQM 245

Query: 1095 PTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSV--DSDI 1268
            PT V+ MLKYGTNLLQAVGQF GHY+++VAY++  SLPE PVL+DYVQPA+TSV  DSD+
Sbjct: 246  PTPVSGMLKYGTNLLQAVGQFTGHYVVIVAYMSFVSLPEDPVLQDYVQPAVTSVDTDSDL 305

Query: 1269 IEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCYT 1448
            IEG SRISLNCPISF RIKTPVKG SCKHFQCFDFDNFINMN +RP WRCPHCNQ V Y 
Sbjct: 306  IEGASRISLNCPISFARIKTPVKGCSCKHFQCFDFDNFINMNSRRPWWRCPHCNQHVSYE 365

Query: 1449 DIRLDRNMIEILKNVGENIVEVIIHADGSW-----------KTVLENAQDVGKMQNKARD 1595
            DIRLDRNM+EILK+VGENI+EVI+  DGSW           KTV E   +V K QNKA +
Sbjct: 366  DIRLDRNMVEILKDVGENILEVIVLGDGSWKAVFEKDHNVDKTVFEEDHNVDKTQNKAHN 425

Query: 1596 CEKEQTELRDSTCSPNS-APDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNST 1763
             EK+  EL++STCSPN+   ++ DLTNDD+ +DIMDT    D KP   S  +QFV  +ST
Sbjct: 426  GEKKPVELQESTCSPNNIVSNIFDLTNDDDCMDIMDTIELEDTKPVPGSAETQFVNQSST 485

Query: 1764 SLSVNSTGVHQNVAAQIDN-YWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGH 1940
            SL +NST V QNVA+ ID+ +WAG+ +   R DTP++G+SE+PVLP +VSPA NQ +  H
Sbjct: 486  SLGLNSTDVDQNVASHIDDEFWAGLDLVFGRSDTPSLGISENPVLPDSVSPAFNQGSNSH 545

Query: 1941 ANIPXXXXXXXXXXXXXXXVQLLNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQ 2120
             N                 +QL+  MNS V+EY  GRS+S PRH+ RT V VQA    S 
Sbjct: 546  DNNSAVNSLVHNQVSAPTNLQLI-YMNSSVNEY--GRSASIPRHVNRTPVAVQALAAPSS 602

Query: 2121 ASGLQQSTITNLDSLLISSSPATPHV--XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSG 2294
             SG +QS    L+S L SSSPAT HV              D ERQQ FSRS +N PQ+S 
Sbjct: 603  ISGREQSLGNTLNSSLPSSSPATTHVPLSHTATAANPILTDTERQQHFSRSQMNLPQMS- 661

Query: 2295 VGSSTPTSQHQSATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPMQ 2474
             G+S+    H SATQ RVPP N  AP QL +  R    L E  NPHL Q+L+ + H P+ 
Sbjct: 662  -GASSLGLPHHSATQNRVPPPNNPAPNQLPNSNRPWVGLGELSNPHLSQSLSSRAH-PVM 719

Query: 2475 TSNTHRSYIQQGG----SQGRTLNGQQARVMASSQVSRQGERGPV--QSVSRTDELNN 2630
             +N  RS+IQQGG    S G T N QQ R  A  QVSR  +RG V  QSVSR D+L N
Sbjct: 720  RTNIQRSHIQQGGSVAQSTGTTANSQQTRANAIGQVSRD-QRGSVTPQSVSRPDDLLN 776


>ref|XP_020216462.1| E4 SUMO-protein ligase PIAL2-like isoform X2 [Cajanus cajan]
          Length = 877

 Score =  820 bits (2117), Expect = 0.0
 Identities = 454/749 (60%), Positives = 535/749 (71%), Gaps = 13/749 (1%)
 Frame = +3

Query: 369  MNNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLS 548
            MN S  L + PD       SPS VN+YRI  V +RL+   Q  S    P+EF+NLCLSLS
Sbjct: 1    MNTSSALPLLPDT------SPSTVNMYRISKVADRLAWLAQTGSRGDHPYEFYNLCLSLS 54

Query: 549  RGIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKES 728
            RGIDYA+ANGE P + ++LP+L+KQI +  NDE+SQAA+MVLMISVK+AC+IGWF+ KES
Sbjct: 55   RGIDYAVANGENPAKAHDLPLLVKQICKLKNDELSQAAMMVLMISVKSACEIGWFQTKES 114

Query: 729  EELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGAS 908
            EEL+TIADE+GKVY + G +N GPSS  S + TIM +FYPKIKLGPIL SI+AKPGYGA 
Sbjct: 115  EELVTIADEIGKVYSSSGILNAGPSSCSSAIPTIMEKFYPKIKLGPILTSIDAKPGYGAF 174

Query: 909  AIDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGP 1088
            A+DFHITKNK L D KI+L VAQTDNIETS CLISPQ+VNFLLNGK +  RTNV+MDPGP
Sbjct: 175  AVDFHITKNKVLKD-KIYLLVAQTDNIETSTCLISPQQVNFLLNGKAVTNRTNVQMDPGP 233

Query: 1089 QMPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSV--DS 1262
            QMPTNVT MLK+GTNLLQAVGQFN HYI+LVAY++  SLPE PVL+DY+QP +TSV  DS
Sbjct: 234  QMPTNVTGMLKFGTNLLQAVGQFNSHYIVLVAYLSVASLPEDPVLQDYLQPTVTSVDSDS 293

Query: 1263 DIIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVC 1442
            DIIEG S+ISLNCPISFTRIKTPVKG SCKHFQCFDFDNFI+MN KRPSWRCPHCNQ VC
Sbjct: 294  DIIEGASQISLNCPISFTRIKTPVKGRSCKHFQCFDFDNFISMNSKRPSWRCPHCNQYVC 353

Query: 1443 YTDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELR 1622
            Y DIRLDR M+EILKNVGENI EVI+ ADGSWK VLE  +DV KMQNKA +CEKEQTE +
Sbjct: 354  YADIRLDRVMVEILKNVGENITEVIVLADGSWKAVLEKDRDVDKMQNKAPNCEKEQTEPQ 413

Query: 1623 DSTCSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVH 1793
            +STCSP++    +DLT DD+ L+IMD+C  ADRKP   SV SQFVTPNSTSL +NSTG++
Sbjct: 414  ESTCSPST----VDLTKDDDCLEIMDSCEIADRKPFQASVHSQFVTPNSTSLGMNSTGIN 469

Query: 1794 QNVAAQI-DNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXX 1970
            QNVAAQI D++W+G+YV          G SE PVLP TVSPA NQE  G  N P      
Sbjct: 470  QNVAAQIEDDFWSGIYV---------TGGSELPVLPDTVSPAFNQEPAGRDNNPVVNSAM 520

Query: 1971 XXXXXXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTI 2147
                     +Q+ LN MNSV    ++GRSSS PR I RT V VQA P+QSQA G QQ++I
Sbjct: 521  HNSFSATNNLQMQLNHMNSV---NEYGRSSSMPR-INRTPVAVQALPVQSQALGPQQNSI 576

Query: 2148 TNLDSLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPT 2315
            TNL     ++S ATPH+                D ERQQ FSRS  N PQVSGV S+   
Sbjct: 577  TNL-----NNSAATPHINLSNPASVDAISAILSDTERQQHFSRSPRNLPQVSGVNST--A 629

Query: 2316 SQHQSATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQ--QHQPMQTSNTH 2489
             QH SAT               Q+PYR  +  +EF+NPHLQQALNP+  Q Q +      
Sbjct: 630  FQHHSAT---------------QNPYRGRS-FSEFKNPHLQQALNPRPPQQQALNPRPPQ 673

Query: 2490 RSYIQQGGSQGRTLNGQQARVMASSQVSR 2576
            +  +     Q ++LN +      SS  S+
Sbjct: 674  QQSLNSRPPQQQSLNSRPPPPTRSSNDSQ 702


>gb|KRH27884.1| hypothetical protein GLYMA_11G020900 [Glycine max]
          Length = 842

 Score =  804 bits (2077), Expect = 0.0
 Identities = 441/746 (59%), Positives = 526/746 (70%), Gaps = 9/746 (1%)
 Frame = +3

Query: 372  NNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLSR 551
            N+SP L +P D       SPS VN++RI  V +RL+   QP  NR +P EF+NLCLSLSR
Sbjct: 3    NSSPALTLPSDT------SPSVVNMFRINKVADRLAWIAQPG-NRGEPHEFYNLCLSLSR 55

Query: 552  GIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKESE 731
            GIDYALANGETPP+ ++LP+L+KQI Q  NDE SQAA+MVL+IS+KNAC+IGWF+ KESE
Sbjct: 56   GIDYALANGETPPKAHDLPLLVKQICQLKNDECSQAAMMVLLISIKNACEIGWFQTKESE 115

Query: 732  ELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGASA 911
            EL++IADE+GKVY +LG +N  P S  + ++TIM +FYPK KLGPIL SIEA+PGYGASA
Sbjct: 116  ELVSIADEIGKVYSSLGTINVRPRSCSTVISTIMQKFYPKFKLGPILASIEAQPGYGASA 175

Query: 912  IDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGPQ 1091
            +DFHITK++ L DK I+L VAQTDNIET ACLISPQ+VNFLLNGKG+  RTNV+MDPG Q
Sbjct: 176  VDFHITKSEVLKDK-IFLLVAQTDNIETPACLISPQQVNFLLNGKGVLNRTNVQMDPGAQ 234

Query: 1092 MPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSVDSDII 1271
            +PTNVT MLK+GTNLLQAVGQFN                                DSDII
Sbjct: 235  VPTNVTGMLKFGTNLLQAVGQFN--------------------------------DSDII 262

Query: 1272 EGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCYTD 1451
            EG SRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFIN+N KRPSWRCP C Q+VCY D
Sbjct: 263  EGASRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFININSKRPSWRCPRCIQNVCYAD 322

Query: 1452 IRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELRDST 1631
            IRLDRNM+EILKNVGENI EVI+ A+GSWK VLE   DV KMQ KA +CEKEQT+ ++ST
Sbjct: 323  IRLDRNMVEILKNVGENITEVIVFANGSWKAVLEKDHDVDKMQKKAPNCEKEQTQPQEST 382

Query: 1632 CSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVHQNV 1802
            C P++    +DLT DD+ LD + +C   +RKP   S+ S FV+PN TSL +NSTGV+QNV
Sbjct: 383  CPPST----VDLTKDDDGLDTVGSCDIVERKPPPASIHSHFVSPNLTSLGMNSTGVNQNV 438

Query: 1803 AAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXXXXX 1982
            AAQ D++W GVY+  S  DTPTVG SE PVLP TVSPA +QE+ G  N P          
Sbjct: 439  AAQTDDFWTGVYIGRSSSDTPTVGNSELPVLPDTVSPAFSQESAGRDNNPVVNSAMHNQF 498

Query: 1983 XXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTITNLD 2159
                 +Q+ +N MNSV    ++GRSSSAPRHI RT V VQA P+QSQA G Q+++ITNL+
Sbjct: 499  SGPSNLQMQMNHMNSV---NEYGRSSSAPRHIHRTPVAVQALPVQSQALGPQENSITNLN 555

Query: 2160 -SLLISSSPATPHV----XXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPTSQH 2324
             SLL S+S A PH+                D ERQQ FSR+ +NPPQVSGV S  P  QH
Sbjct: 556  SSLLPSNSSAAPHISLSNPASVDTLNAILSDTERQQHFSRTPVNPPQVSGVNS--PAFQH 613

Query: 2325 QSATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALNPQQHQPMQTSNTHRSYIQ 2504
             +ATQ RVP +N S PTQ Q+ YRA    +EFRN HLQQALN +   P  +SNT  S+IQ
Sbjct: 614  HTATQNRVPLINTSVPTQPQNQYRANV-FSEFRNSHLQQALN-RWPPPSTSSNTQWSHIQ 671

Query: 2505 QGGSQGRTLNGQQARVMASSQVSRQG 2582
            Q   Q       Q      +  +RQG
Sbjct: 672  QSVPQSGNF---QVAARGGALAARQG 694


>ref|XP_017407252.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X2 [Vigna angularis]
 gb|KOM27106.1| hypothetical protein LR48_Vigan401s001600 [Vigna angularis]
          Length = 842

 Score =  787 bits (2033), Expect = 0.0
 Identities = 427/745 (57%), Positives = 520/745 (69%), Gaps = 7/745 (0%)
 Frame = +3

Query: 369  MNNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLS 548
            MN +  L +P +       SPS +N+YRI  V ERL+   QP  NR DP EF+N CLSLS
Sbjct: 1    MNGAVGLPLPANS------SPSVINMYRIQKVTERLAWLAQPG-NRGDPKEFYNHCLSLS 53

Query: 549  RGIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKES 728
            RGIDYALANGE P   +ELP+L+KQI Q  NDE+SQAA+MVLMISVK AC+IGWF+ KES
Sbjct: 54   RGIDYALANGEIPSNAHELPLLVKQICQLKNDELSQAAMMVLMISVKGACEIGWFQTKES 113

Query: 729  EELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGAS 908
            +ELLTI DE+GKVY ++G++N       S ++TIM +FYP +KLG IL SIE +PGYGAS
Sbjct: 114  QELLTILDEIGKVYSSMGSINATSRQCSSEISTIMEKFYPHVKLGSILASIEIQPGYGAS 173

Query: 909  AIDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGP 1088
             + FHITK++F+ +K ++L VAQ DNIE SACLISP + NFLLNGKGI  RT V MDPGP
Sbjct: 174  LVGFHITKSEFVKEK-LFLLVAQIDNIEISACLISPPQANFLLNGKGIANRTYVEMDPGP 232

Query: 1089 QMPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSVDSDI 1268
            QMPT+VT+MLK+GTNLLQAVGQFNG YI+LVAY++ T L E PVL+DY+QPA+TS+D DI
Sbjct: 233  QMPTDVTAMLKFGTNLLQAVGQFNGRYIVLVAYMSFTPLHEDPVLQDYLQPAVTSIDFDI 292

Query: 1269 IEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCYT 1448
            +EG S+ISLNCPISFTRIKTPVKG SCKHFQCFDF+NFI++N KRPSWRCPHCNQ VCY 
Sbjct: 293  VEGASQISLNCPISFTRIKTPVKGRSCKHFQCFDFNNFISINSKRPSWRCPHCNQYVCYA 352

Query: 1449 DIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELRDS 1628
            DIRLDRNM+EILKNVGE+I EVI+ ADGSWK V E   DV KMQ KA + EKEQTE ++ 
Sbjct: 353  DIRLDRNMVEILKNVGESITEVIVLADGSWKAVSEKDHDVDKMQKKAPNHEKEQTEPQEY 412

Query: 1629 TCSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVHQN 1799
            TCSP +    +DLT DD+H++ MD+    DRKP   S+ S FV PNSTSL +N+ G+++N
Sbjct: 413  TCSPAT----VDLTEDDDHVEAMDSSEIVDRKPLLASIQSHFVAPNSTSLGMNTPGINRN 468

Query: 1800 VAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXXXX 1979
            VA Q+D++ +GVY+A SR DTP VG  E P+LP T+SPA NQE+ G  N P         
Sbjct: 469  VAGQMDDFLSGVYIACSRSDTPMVGTLELPILPDTISPAFNQESAGRDNNPAVNSGMRNQ 528

Query: 1980 XXXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTITNL 2156
                  + + +N MNSV    ++GRSSS PRHI RT V VQA P+QSQASG     I + 
Sbjct: 529  ISGPNNLPMQMNHMNSV---NEYGRSSSVPRHINRTPVAVQALPVQSQASGDTLKAILS- 584

Query: 2157 DSLLISSSPATPHVXXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPTSQHQSAT 2336
                                      D ERQQ FSRS +N PQVSGV S  P  +H +AT
Sbjct: 585  --------------------------DTERQQRFSRSPMNLPQVSGVNS--PAFRHHNAT 616

Query: 2337 QIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALN---PQQHQPMQTSNTHRSYIQQ 2507
            Q RVP  N    TQ Q+PYR P  L++FRN HLQQ+LN    +QH   + S T  S+IQQ
Sbjct: 617  QSRVPLQNPPTSTQSQNPYR-PGSLSDFRNSHLQQSLNIRPSRQHSNFRPSTTPWSHIQQ 675

Query: 2508 GGSQGRTLNGQQARVMASSQVSRQG 2582
               Q   L   QA  MA+   +RQG
Sbjct: 676  SVPQSGNL---QAAGMAAPPAARQG 697


>ref|XP_022643149.1| E4 SUMO-protein ligase PIAL2 isoform X2 [Vigna radiata var. radiata]
          Length = 842

 Score =  785 bits (2026), Expect = 0.0
 Identities = 423/745 (56%), Positives = 519/745 (69%), Gaps = 7/745 (0%)
 Frame = +3

Query: 369  MNNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLS 548
            MN +  L +P +       SPS +N+YRI  V ERL    QP  NR DP EF+N CLSLS
Sbjct: 1    MNGAVGLPLPANS------SPSVINMYRIQKVTERLGWLAQPG-NRGDPKEFYNHCLSLS 53

Query: 549  RGIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKES 728
            RGIDYALANGE P   +ELP+L+K+I Q  NDE+SQAA+MVLMISVK AC+IGWF+ KES
Sbjct: 54   RGIDYALANGEIPTNAHELPLLVKKICQLKNDELSQAAMMVLMISVKGACEIGWFQTKES 113

Query: 729  EELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGAS 908
            +ELLTI DE+GKV+ ++G++N  P    S ++TIM +FYP +KLG IL SIE +PGYGAS
Sbjct: 114  QELLTILDEIGKVFSSMGSINATPRQCSSEISTIMEKFYPHVKLGSILASIEIQPGYGAS 173

Query: 909  AIDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGP 1088
             + FHITK++F+ +K ++L VAQ DNIE SACLISP + NFLLNGKG+  RT V MDPGP
Sbjct: 174  LVGFHITKSEFVKEK-LFLLVAQIDNIEISACLISPPQANFLLNGKGVANRTYVEMDPGP 232

Query: 1089 QMPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSVDSDI 1268
            QMPT+VT+MLK+GTNLLQ VGQFNG YI+LVAY++ T L E PVL+DY+QPA+TS DSDI
Sbjct: 233  QMPTDVTAMLKFGTNLLQTVGQFNGRYIVLVAYMSFTPLHEDPVLQDYLQPAVTSTDSDI 292

Query: 1269 IEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVCYT 1448
            +EG S+ISLNCPISFTRIKTPVKG SCKHFQCFDF+NF+++N KRPSWRCPHCNQ VCY 
Sbjct: 293  VEGASQISLNCPISFTRIKTPVKGRSCKHFQCFDFNNFLSINSKRPSWRCPHCNQYVCYA 352

Query: 1449 DIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELRDS 1628
            DIRLDRNM+EILKNVGE+I EVI+ ADGSWK VLE   DV KMQ KA + EKEQTE ++ 
Sbjct: 353  DIRLDRNMVEILKNVGESITEVIVLADGSWKAVLEKDHDVDKMQKKAPNHEKEQTEPQEH 412

Query: 1629 TCSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVHQN 1799
            TCSP +    +DLT DD+H++ MDT    DRKP   S+ S FV PNSTS  +N+ GV++N
Sbjct: 413  TCSPAT----VDLTEDDDHVEAMDTSEIVDRKPLQASLQSHFVAPNSTSFGMNTPGVNRN 468

Query: 1800 VAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXXXX 1979
            VA Q+D++ +GVY+A SR DTP VG  E PVLP T+SP  NQE+ G  N           
Sbjct: 469  VAGQMDDFLSGVYIARSRSDTPMVGTLELPVLPDTISPTFNQESAGRDNNSAVNSAMRNQ 528

Query: 1980 XXXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTITNL 2156
                  + + +N MNSV    ++GRSSS PRHI R  V VQA P+QSQASG     I + 
Sbjct: 529  ISAPNNLPMQMNQMNSV---NEYGRSSSVPRHINRIPVAVQALPVQSQASGDTLKAILS- 584

Query: 2157 DSLLISSSPATPHVXXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPTSQHQSAT 2336
                                      D ERQQ FSR+ +N PQVSGV S  P  +H +AT
Sbjct: 585  --------------------------DTERQQRFSRTPINLPQVSGVNS--PAFRHHNAT 616

Query: 2337 QIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALN---PQQHQPMQTSNTHRSYIQQ 2507
            Q RVP  N    TQLQ+PYR P+ +++F NPHLQQ+LN    +QH   + S T  S+IQQ
Sbjct: 617  QNRVPLQNPPTSTQLQNPYR-PSSISDFSNPHLQQSLNLLPSRQHSNFRPSTTPWSHIQQ 675

Query: 2508 GGSQGRTLNGQQARVMASSQVSRQG 2582
            G  Q   L   +A  MA+   +RQG
Sbjct: 676  GVPQSGNL---KAAGMAAPPAARQG 697


>ref|XP_017407251.1| PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vigna angularis]
 dbj|BAU00598.1| hypothetical protein VIGAN_10220700 [Vigna angularis var. angularis]
          Length = 844

 Score =  785 bits (2026), Expect = 0.0
 Identities = 428/747 (57%), Positives = 521/747 (69%), Gaps = 9/747 (1%)
 Frame = +3

Query: 369  MNNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLS 548
            MN +  L +P +       SPS +N+YRI  V ERL+   QP  NR DP EF+N CLSLS
Sbjct: 1    MNGAVGLPLPANS------SPSVINMYRIQKVTERLAWLAQPG-NRGDPKEFYNHCLSLS 53

Query: 549  RGIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKES 728
            RGIDYALANGE P   +ELP+L+KQI Q  NDE+SQAA+MVLMISVK AC+IGWF+ KES
Sbjct: 54   RGIDYALANGEIPSNAHELPLLVKQICQLKNDELSQAAMMVLMISVKGACEIGWFQTKES 113

Query: 729  EELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGAS 908
            +ELLTI DE+GKVY ++G++N       S ++TIM +FYP +KLG IL SIE +PGYGAS
Sbjct: 114  QELLTILDEIGKVYSSMGSINATSRQCSSEISTIMEKFYPHVKLGSILASIEIQPGYGAS 173

Query: 909  AIDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGP 1088
             + FHITK++F+ +K ++L VAQ DNIE SACLISP + NFLLNGKGI  RT V MDPGP
Sbjct: 174  LVGFHITKSEFVKEK-LFLLVAQIDNIEISACLISPPQANFLLNGKGIANRTYVEMDPGP 232

Query: 1089 QMPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSVD--S 1262
            QMPT+VT+MLK+GTNLLQAVGQFNG YI+LVAY++ T L E PVL+DY+QPA+TS+D  S
Sbjct: 233  QMPTDVTAMLKFGTNLLQAVGQFNGRYIVLVAYMSFTPLHEDPVLQDYLQPAVTSIDFDS 292

Query: 1263 DIIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVC 1442
            DI+EG S+ISLNCPISFTRIKTPVKG SCKHFQCFDF+NFI++N KRPSWRCPHCNQ VC
Sbjct: 293  DIVEGASQISLNCPISFTRIKTPVKGRSCKHFQCFDFNNFISINSKRPSWRCPHCNQYVC 352

Query: 1443 YTDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELR 1622
            Y DIRLDRNM+EILKNVGE+I EVI+ ADGSWK V E   DV KMQ KA + EKEQTE +
Sbjct: 353  YADIRLDRNMVEILKNVGESITEVIVLADGSWKAVSEKDHDVDKMQKKAPNHEKEQTEPQ 412

Query: 1623 DSTCSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVH 1793
            + TCSP +    +DLT DD+H++ MD+    DRKP   S+ S FV PNSTSL +N+ G++
Sbjct: 413  EYTCSPAT----VDLTEDDDHVEAMDSSEIVDRKPLLASIQSHFVAPNSTSLGMNTPGIN 468

Query: 1794 QNVAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXX 1973
            +NVA Q+D++ +GVY+A SR DTP VG  E P+LP T+SPA NQE+ G  N P       
Sbjct: 469  RNVAGQMDDFLSGVYIACSRSDTPMVGTLELPILPDTISPAFNQESAGRDNNPAVNSGMR 528

Query: 1974 XXXXXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTIT 2150
                    + + +N MNSV    ++GRSSS PRHI RT V VQA P+QSQASG     I 
Sbjct: 529  NQISGPNNLPMQMNHMNSV---NEYGRSSSVPRHINRTPVAVQALPVQSQASGDTLKAIL 585

Query: 2151 NLDSLLISSSPATPHVXXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPTSQHQS 2330
            +                           D ERQQ FSRS +N PQVSGV S  P  +H +
Sbjct: 586  S---------------------------DTERQQRFSRSPMNLPQVSGVNS--PAFRHHN 616

Query: 2331 ATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALN---PQQHQPMQTSNTHRSYI 2501
            ATQ RVP  N    TQ Q+PYR P  L++FRN HLQQ+LN    +QH   + S T  S+I
Sbjct: 617  ATQSRVPLQNPPTSTQSQNPYR-PGSLSDFRNSHLQQSLNIRPSRQHSNFRPSTTPWSHI 675

Query: 2502 QQGGSQGRTLNGQQARVMASSQVSRQG 2582
            QQ   Q   L   QA  MA+   +RQG
Sbjct: 676  QQSVPQSGNL---QAAGMAAPPAARQG 699


>ref|XP_014521171.1| E4 SUMO-protein ligase PIAL2 isoform X1 [Vigna radiata var. radiata]
          Length = 844

 Score =  780 bits (2013), Expect = 0.0
 Identities = 423/747 (56%), Positives = 519/747 (69%), Gaps = 9/747 (1%)
 Frame = +3

Query: 369  MNNSPPLGMPPDMGTGKQMSPSFVNLYRIMAVVERLSMHVQPNSNRTDPFEFFNLCLSLS 548
            MN +  L +P +       SPS +N+YRI  V ERL    QP  NR DP EF+N CLSLS
Sbjct: 1    MNGAVGLPLPANS------SPSVINMYRIQKVTERLGWLAQPG-NRGDPKEFYNHCLSLS 53

Query: 549  RGIDYALANGETPPQVNELPMLMKQIYQRNNDEISQAAVMVLMISVKNACKIGWFEMKES 728
            RGIDYALANGE P   +ELP+L+K+I Q  NDE+SQAA+MVLMISVK AC+IGWF+ KES
Sbjct: 54   RGIDYALANGEIPTNAHELPLLVKKICQLKNDELSQAAMMVLMISVKGACEIGWFQTKES 113

Query: 729  EELLTIADELGKVYCTLGNMNTGPSSYHSTVTTIMARFYPKIKLGPILVSIEAKPGYGAS 908
            +ELLTI DE+GKV+ ++G++N  P    S ++TIM +FYP +KLG IL SIE +PGYGAS
Sbjct: 114  QELLTILDEIGKVFSSMGSINATPRQCSSEISTIMEKFYPHVKLGSILASIEIQPGYGAS 173

Query: 909  AIDFHITKNKFLPDKKIWLFVAQTDNIETSACLISPQEVNFLLNGKGIDTRTNVRMDPGP 1088
             + FHITK++F+ +K ++L VAQ DNIE SACLISP + NFLLNGKG+  RT V MDPGP
Sbjct: 174  LVGFHITKSEFVKEK-LFLLVAQIDNIEISACLISPPQANFLLNGKGVANRTYVEMDPGP 232

Query: 1089 QMPTNVTSMLKYGTNLLQAVGQFNGHYIILVAYINATSLPEHPVLEDYVQPAITSVDSD- 1265
            QMPT+VT+MLK+GTNLLQ VGQFNG YI+LVAY++ T L E PVL+DY+QPA+TS DSD 
Sbjct: 233  QMPTDVTAMLKFGTNLLQTVGQFNGRYIVLVAYMSFTPLHEDPVLQDYLQPAVTSTDSDS 292

Query: 1266 -IIEGPSRISLNCPISFTRIKTPVKGHSCKHFQCFDFDNFINMNFKRPSWRCPHCNQSVC 1442
             I+EG S+ISLNCPISFTRIKTPVKG SCKHFQCFDF+NF+++N KRPSWRCPHCNQ VC
Sbjct: 293  DIVEGASQISLNCPISFTRIKTPVKGRSCKHFQCFDFNNFLSINSKRPSWRCPHCNQYVC 352

Query: 1443 YTDIRLDRNMIEILKNVGENIVEVIIHADGSWKTVLENAQDVGKMQNKARDCEKEQTELR 1622
            Y DIRLDRNM+EILKNVGE+I EVI+ ADGSWK VLE   DV KMQ KA + EKEQTE +
Sbjct: 353  YADIRLDRNMVEILKNVGESITEVIVLADGSWKAVLEKDHDVDKMQKKAPNHEKEQTEPQ 412

Query: 1623 DSTCSPNSAPDVLDLTNDDNHLDIMDTCATADRKP---SVPSQFVTPNSTSLSVNSTGVH 1793
            + TCSP +    +DLT DD+H++ MDT    DRKP   S+ S FV PNSTS  +N+ GV+
Sbjct: 413  EHTCSPAT----VDLTEDDDHVEAMDTSEIVDRKPLQASLQSHFVAPNSTSFGMNTPGVN 468

Query: 1794 QNVAAQIDNYWAGVYVANSRPDTPTVGVSEHPVLPGTVSPAINQEAEGHANIPXXXXXXX 1973
            +NVA Q+D++ +GVY+A SR DTP VG  E PVLP T+SP  NQE+ G  N         
Sbjct: 469  RNVAGQMDDFLSGVYIARSRSDTPMVGTLELPVLPDTISPTFNQESAGRDNNSAVNSAMR 528

Query: 1974 XXXXXXXXVQL-LNLMNSVVDEYQHGRSSSAPRHIGRTSVGVQARPIQSQASGLQQSTIT 2150
                    + + +N MNSV    ++GRSSS PRHI R  V VQA P+QSQASG     I 
Sbjct: 529  NQISAPNNLPMQMNQMNSV---NEYGRSSSVPRHINRIPVAVQALPVQSQASGDTLKAIL 585

Query: 2151 NLDSLLISSSPATPHVXXXXXXXXXXXXDAERQQLFSRSSLNPPQVSGVGSSTPTSQHQS 2330
            +                           D ERQQ FSR+ +N PQVSGV S  P  +H +
Sbjct: 586  S---------------------------DTERQQRFSRTPINLPQVSGVNS--PAFRHHN 616

Query: 2331 ATQIRVPPVNMSAPTQLQSPYRAPARLNEFRNPHLQQALN---PQQHQPMQTSNTHRSYI 2501
            ATQ RVP  N    TQLQ+PYR P+ +++F NPHLQQ+LN    +QH   + S T  S+I
Sbjct: 617  ATQNRVPLQNPPTSTQLQNPYR-PSSISDFSNPHLQQSLNLLPSRQHSNFRPSTTPWSHI 675

Query: 2502 QQGGSQGRTLNGQQARVMASSQVSRQG 2582
            QQG  Q   L   +A  MA+   +RQG
Sbjct: 676  QQGVPQSGNL---KAAGMAAPPAARQG 699