BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00005378
         (499 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|GAU16173.1| hypothetical protein TSUD_298030 [Trifolium subt...   247   3e-77
ref|XP_004486593.1| PREDICTED: 4-coumarate--CoA ligase 1 [Cicer ...   248   1e-76
gb|PNX95884.1| 4-coumarate CoA ligase, partial [Trifolium pratense]   244   1e-76
gb|AFK47607.1| unknown [Medicago truncatula]                          243   5e-75
ref|XP_003604127.1| 4-coumarate:CoA ligase-like protein [Medicag...   243   5e-75
dbj|GAU16187.1| hypothetical protein TSUD_298180 [Trifolium subt...   239   1e-73
gb|AFR37186.1| 4-coumarate CoA ligase, partial [Populus alba]         224   1e-72
gb|AFR37181.1| 4-coumarate CoA ligase, partial [Populus alba] >g...   224   1e-72
ref|XP_004507544.1| PREDICTED: 4-coumarate--CoA ligase 1-like [C...   235   5e-72
gb|AFR37187.1| 4-coumarate CoA ligase, partial [Populus alba]         223   8e-72
gb|ACZ64784.1| 4-coumarate:CoA ligase [Galega orientalis]             234   8e-72
gb|AFR37197.1| 4-coumarate CoA ligase, partial [Populus fremontii]    221   2e-71
gb|AFR37188.1| 4-coumarate CoA ligase, partial [Populus fremonti...   221   2e-71
gb|AAL35216.1| 4-coumarate:CoA ligase [Amorpha fruticosa]             232   5e-71
gb|ADF30254.1| p-coumarate:CoA ligase 1 [Sorbus aucuparia]            232   6e-71
gb|ACI23349.1| 4-coumarate CoA ligase 2, partial [Leucaena leuco...   231   6e-71
ref|XP_022153393.1| 4-coumarate--CoA ligase 1-like [Momordica ch...   230   7e-71
gb|AHA42446.1| 4-coumarate:CoA ligase, partial [Cannabis sativa]      228   8e-71
gb|AFR37166.1| 4-coumarate CoA ligase, partial [Populus trichoca...   220   9e-71
ref|XP_009354316.1| PREDICTED: 4-coumarate--CoA ligase 1-like [P...   231   1e-70

>dbj|GAU16173.1| hypothetical protein TSUD_298030 [Trifolium subterraneum]
          Length = 505

 Score =  247 bits (631), Expect = 3e-77
 Identities = 127/173 (73%), Positives = 136/173 (78%), Gaps = 7/173 (4%)
 Frame = +1

Query: 1   ESNHHHHV-------ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQV 159
           E  +H H        EN+  EV IN DDVVALPYSSGTTGLPKGVMLTHKGLVTS+AQQV
Sbjct: 148 EGENHMHFRELAQADENEIEEVKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQV 207

Query: 160 DGENPNLYYSTEDVILCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYR 339
           DGENPNLYY +EDVILCVLP+FHIYSLNSVLLCGLRAKA+IL MPKF+I  F  LV+KYR
Sbjct: 208 DGENPNLYYHSEDVILCVLPMFHIYSLNSVLLCGLRAKASILLMPKFDIHAFFSLVHKYR 267

Query: 340 XXXXXXXXXXXXXXXKSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
                          KSPELDNYDLSSIR+LKSGGAPLGKELE TVRAKFPKA
Sbjct: 268 VTIVPVVPPIVLAISKSPELDNYDLSSIRVLKSGGAPLGKELEDTVRAKFPKA 320


>ref|XP_004486593.1| PREDICTED: 4-coumarate--CoA ligase 1 [Cicer arietinum]
          Length = 568

 Score =  248 bits (632), Expect = 1e-76
 Identities = 125/158 (79%), Positives = 133/158 (84%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           EN+ GEV I GDDVVALPYSSGTTGLPKGVMLTHKGLV+SIAQQVDGENPNLYY+ +DVI
Sbjct: 195 ENEVGEVKIEGDDVVALPYSSGTTGLPKGVMLTHKGLVSSIAQQVDGENPNLYYTCDDVI 254

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLPLFHIYSLNSVLLCGLRAK+TIL MPKFEI  FL LV+K+R               
Sbjct: 255 LCVLPLFHIYSLNSVLLCGLRAKSTILLMPKFEINAFLTLVHKHRVTVAPVVPPIVLAIA 314

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KSP+L NYDLSSIRILKSGGAPLGKELEHTVRAKFP A
Sbjct: 315 KSPDLQNYDLSSIRILKSGGAPLGKELEHTVRAKFPNA 352


>gb|PNX95884.1| 4-coumarate CoA ligase, partial [Trifolium pratense]
          Length = 467

 Score =  244 bits (624), Expect = 1e-76
 Identities = 125/173 (72%), Positives = 135/173 (78%), Gaps = 7/173 (4%)
 Frame = +1

Query: 1   ESNHHHHV-------ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQV 159
           E  +H H        EN+  E  IN DDVVALPYSSGTTGLPKGVMLTHKGLVTS+AQQV
Sbjct: 153 EGENHMHFRELAQADENEIEEAKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQV 212

Query: 160 DGENPNLYYSTEDVILCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYR 339
           DGENPNLYY +EDVILCVLP+FHIYSLNSVLLCGLRAKA+IL MPKF+I  F  LV+KY+
Sbjct: 213 DGENPNLYYHSEDVILCVLPMFHIYSLNSVLLCGLRAKASILLMPKFDINAFFSLVHKYK 272

Query: 340 XXXXXXXXXXXXXXXKSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
                          KSPELDNYDLSSIR+LKSGGAPLGKELE TVRAKFPKA
Sbjct: 273 VTIAPVVPPIVLAISKSPELDNYDLSSIRVLKSGGAPLGKELEDTVRAKFPKA 325


>gb|AFK47607.1| unknown [Medicago truncatula]
          Length = 544

 Score =  243 bits (619), Expect = 5e-75
 Identities = 128/174 (73%), Positives = 135/174 (77%), Gaps = 8/174 (4%)
 Frame = +1

Query: 1   ESNHHHHVE------NDFGEV--NINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQ 156
           E NH H  E      N+  EV  NI  DDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQ
Sbjct: 155 EDNHMHFSELIQADQNEMEEVKVNIKPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQ 214

Query: 157 VDGENPNLYYSTEDVILCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKY 336
           VDGENPNLYY +EDVILCVLP+FHIYSLNSVLLCGLRAKA+IL MPKF+I  F GLV KY
Sbjct: 215 VDGENPNLYYHSEDVILCVLPMFHIYSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKY 274

Query: 337 RXXXXXXXXXXXXXXXKSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           +               KSPELD YDLSSIR+LKSGGAPLGKELE TVRAKFPKA
Sbjct: 275 KVTLAPVVPPIVLAIAKSPELDKYDLSSIRVLKSGGAPLGKELEDTVRAKFPKA 328


>ref|XP_003604127.1| 4-coumarate:CoA ligase-like protein [Medicago truncatula]
 gb|AES86324.1| 4-coumarate:CoA ligase-like protein [Medicago truncatula]
          Length = 544

 Score =  243 bits (619), Expect = 5e-75
 Identities = 128/174 (73%), Positives = 135/174 (77%), Gaps = 8/174 (4%)
 Frame = +1

Query: 1   ESNHHHHVE------NDFGEV--NINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQ 156
           E NH H  E      N+  EV  NI  DDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQ
Sbjct: 155 EDNHMHFSELIQADQNEMEEVKVNIKPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQ 214

Query: 157 VDGENPNLYYSTEDVILCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKY 336
           VDGENPNLYY +EDVILCVLP+FHIYSLNSVLLCGLRAKA+IL MPKF+I  F GLV KY
Sbjct: 215 VDGENPNLYYHSEDVILCVLPMFHIYSLNSVLLCGLRAKASILLMPKFDINAFFGLVTKY 274

Query: 337 RXXXXXXXXXXXXXXXKSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           +               KSPELD YDLSSIR+LKSGGAPLGKELE TVRAKFPKA
Sbjct: 275 KVTIAPVVPPIVLAIAKSPELDKYDLSSIRVLKSGGAPLGKELEDTVRAKFPKA 328


>dbj|GAU16187.1| hypothetical protein TSUD_298180 [Trifolium subterraneum]
          Length = 536

 Score =  239 bits (609), Expect = 1e-73
 Identities = 122/158 (77%), Positives = 129/158 (81%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           E +  +V I+ DDVVALPYSSGTTGLPKGVMLTHKGLVTSI QQVDGENPNLYYS EDVI
Sbjct: 189 EKELPDVKIHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIGQQVDGENPNLYYSNEDVI 248

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLPLFHIYSLNSVLLCGLRAKATIL MPKF+I GFL LVNK+                
Sbjct: 249 LCVLPLFHIYSLNSVLLCGLRAKATILLMPKFDINGFLSLVNKHGVTVAPVVPPIVLAIA 308

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KSP+L+ YDLSSIRILKSGGAPLGKELE TVR KFPKA
Sbjct: 309 KSPDLNKYDLSSIRILKSGGAPLGKELEDTVRTKFPKA 346


>gb|AFR37186.1| 4-coumarate CoA ligase, partial [Populus alba]
          Length = 169

 Score =  224 bits (572), Expect = 1e-72
 Identities = 108/158 (68%), Positives = 130/158 (82%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           EN+  +V+I+ DDVVALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+ +EDVI
Sbjct: 12  ENEAPQVDISPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVI 71

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLP+FHIY+LNS++LCGLR  A+IL MPKFEI   LGL+ KY+               
Sbjct: 72  LCVLPMFHIYALNSIMLCGLRVGASILIMPKFEIGSLLGLIEKYKVSIAPVVPPVMMSIA 131

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KSP+LD +DLSS+R++KSGGAPLGKELE TVRAKFP+A
Sbjct: 132 KSPDLDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQA 169


>gb|AFR37181.1| 4-coumarate CoA ligase, partial [Populus alba]
 gb|AFR37182.1| 4-coumarate CoA ligase, partial [Populus alba]
 gb|AFR37183.1| 4-coumarate CoA ligase, partial [Populus alba]
 gb|AFR37184.1| 4-coumarate CoA ligase, partial [Populus alba]
          Length = 169

 Score =  224 bits (572), Expect = 1e-72
 Identities = 108/158 (68%), Positives = 130/158 (82%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           EN+  +V+I+ DDVVALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+ +EDVI
Sbjct: 12  ENEAPQVDISPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVI 71

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLP+FHIY+LNS++LCGLR  A+IL MPKFEI   LGL+ KY+               
Sbjct: 72  LCVLPMFHIYALNSIMLCGLRVGASILIMPKFEIGSLLGLIEKYKVSIAPVVPPVMMSIA 131

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KSP+LD +DLSS+R++KSGGAPLGKELE TVRAKFP+A
Sbjct: 132 KSPDLDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQA 169


>ref|XP_004507544.1| PREDICTED: 4-coumarate--CoA ligase 1-like [Cicer arietinum]
          Length = 554

 Score =  235 bits (600), Expect = 5e-72
 Identities = 122/160 (76%), Positives = 129/160 (80%), Gaps = 2/160 (1%)
 Frame = +1

Query: 25  ENDFGEVNI--NGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTED 198
           EN+  EVNI  N DDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYY  ED
Sbjct: 179 ENEMKEVNIKINPDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYHKED 238

Query: 199 VILCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXX 378
           VILCVLP+FHIYSLNSVLLCGLRAKA IL MPKF+I  FL  VNKY+             
Sbjct: 239 VILCVLPMFHIYSLNSVLLCGLRAKAAILLMPKFDINAFLSHVNKYKVTIAPVVPPIVLA 298

Query: 379 XXKSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
             KSP+L  YDLSSIR+LKSGGAPLGKELE +VRAKFPKA
Sbjct: 299 IAKSPDLHKYDLSSIRVLKSGGAPLGKELEDSVRAKFPKA 338


>gb|AFR37187.1| 4-coumarate CoA ligase, partial [Populus alba]
          Length = 169

 Score =  223 bits (567), Expect = 8e-72
 Identities = 107/158 (67%), Positives = 129/158 (81%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           EN+  +V+I+ DDVVALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+ +EDVI
Sbjct: 12  ENEAPQVDISPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVI 71

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLP+FHIY+LNS++LCGLR  A+IL MPKFEI   LG + KY+               
Sbjct: 72  LCVLPMFHIYALNSIMLCGLRVGASILIMPKFEIGSLLGXIEKYKVSIAPVVPPVMMSIA 131

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KSP+LD +DLSS+R++KSGGAPLGKELE TVRAKFP+A
Sbjct: 132 KSPDLDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQA 169


>gb|ACZ64784.1| 4-coumarate:CoA ligase [Galega orientalis]
          Length = 550

 Score =  234 bits (598), Expect = 8e-72
 Identities = 121/153 (79%), Positives = 127/153 (83%)
 Frame = +1

Query: 40  EVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVILCVLP 219
           EV IN +DVVALPYSSGTTGLPKGVMLTHKGLV+SIAQQVDGENPNLYY  EDVILCVLP
Sbjct: 182 EVKINPEDVVALPYSSGTTGLPKGVMLTHKGLVSSIAQQVDGENPNLYYKCEDVILCVLP 241

Query: 220 LFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXXKSPEL 399
           LFHIYSLNSVLLCGLRAKATIL MPKF+I  FL LVNK+                KSP+L
Sbjct: 242 LFHIYSLNSVLLCGLRAKATILLMPKFDINVFLNLVNKHGVSVAPVVPPIVLAIAKSPDL 301

Query: 400 DNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           + YDLSSIRILKSGGAPLGKELE TVRAKFPKA
Sbjct: 302 NKYDLSSIRILKSGGAPLGKELEDTVRAKFPKA 334


>gb|AFR37197.1| 4-coumarate CoA ligase, partial [Populus fremontii]
          Length = 167

 Score =  221 bits (564), Expect = 2e-71
 Identities = 106/158 (67%), Positives = 129/158 (81%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           EN+  +V+ + DDVVALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+ +EDVI
Sbjct: 10  ENEVPQVDFSPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVI 69

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLP+FHIY+LNS++LCGLR  A+IL MPKF+I   LGL+ KY+               
Sbjct: 70  LCVLPMFHIYALNSIMLCGLRVGASILIMPKFDIGTLLGLIEKYKVSIAPVVPPVMLAIA 129

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KSP+LD +DLSS+R++KSGGAPLGKELE TVRAKFP+A
Sbjct: 130 KSPDLDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQA 167


>gb|AFR37188.1| 4-coumarate CoA ligase, partial [Populus fremontii]
 gb|AFR37189.1| 4-coumarate CoA ligase, partial [Populus fremontii]
 gb|AFR37190.1| 4-coumarate CoA ligase, partial [Populus fremontii]
 gb|AFR37192.1| 4-coumarate CoA ligase, partial [Populus fremontii]
 gb|AFR37193.1| 4-coumarate CoA ligase, partial [Populus fremontii]
 gb|AFR37194.1| 4-coumarate CoA ligase, partial [Populus fremontii]
 gb|AFR37195.1| 4-coumarate CoA ligase, partial [Populus fremontii]
 gb|AFR37196.1| 4-coumarate CoA ligase, partial [Populus fremontii]
 gb|AFR37198.1| 4-coumarate CoA ligase, partial [Populus fremontii]
 gb|AFR37200.1| 4-coumarate CoA ligase, partial [Populus fremontii]
 gb|AFR37201.1| 4-coumarate CoA ligase, partial [Populus nigra]
 gb|AFR37203.1| 4-coumarate CoA ligase, partial [Populus nigra]
 gb|AFR37204.1| 4-coumarate CoA ligase, partial [Populus nigra]
 gb|AFR37206.1| 4-coumarate CoA ligase, partial [Populus nigra]
 gb|AFR37208.1| 4-coumarate CoA ligase, partial [Populus nigra]
 gb|AFR37210.1| 4-coumarate CoA ligase, partial [Populus nigra]
 gb|AFR37211.1| 4-coumarate CoA ligase, partial [Populus nigra]
 gb|AFR37212.1| 4-coumarate CoA ligase, partial [Populus nigra]
 gb|AFR37214.1| 4-coumarate CoA ligase, partial [Populus nigra]
 gb|AFR37215.1| 4-coumarate CoA ligase, partial [Populus nigra]
          Length = 169

 Score =  221 bits (564), Expect = 2e-71
 Identities = 106/158 (67%), Positives = 129/158 (81%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           EN+  +V+ + DDVVALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+ +EDVI
Sbjct: 12  ENEVPQVDFSPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVI 71

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLP+FHIY+LNS++LCGLR  A+IL MPKF+I   LGL+ KY+               
Sbjct: 72  LCVLPMFHIYALNSIMLCGLRVGASILIMPKFDIGTLLGLIEKYKVSIAPVVPPVMLAIA 131

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KSP+LD +DLSS+R++KSGGAPLGKELE TVRAKFP+A
Sbjct: 132 KSPDLDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQA 169


>gb|AAL35216.1| 4-coumarate:CoA ligase [Amorpha fruticosa]
          Length = 540

 Score =  232 bits (592), Expect = 5e-71
 Identities = 118/158 (74%), Positives = 126/158 (79%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           E+D  EV  N DDVVALPYSSGTTGLPKGVML+HKGL TSIAQQVDGENPNLY+  EDVI
Sbjct: 167 ESDMPEVKTNPDDVVALPYSSGTTGLPKGVMLSHKGLATSIAQQVDGENPNLYFHNEDVI 226

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLPLFHIYSLNSVLLCGLRAKA IL MPKFEI   LGL+ K+R               
Sbjct: 227 LCVLPLFHIYSLNSVLLCGLRAKAAILLMPKFEINALLGLIQKHRVTIAPIVPPIVLAIA 286

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KSP+L+ YDLSSIR+LKSGGA LGKELE TVRAKFPKA
Sbjct: 287 KSPDLEKYDLSSIRVLKSGGASLGKELEDTVRAKFPKA 324


>gb|ADF30254.1| p-coumarate:CoA ligase 1 [Sorbus aucuparia]
          Length = 547

 Score =  232 bits (592), Expect = 6e-71
 Identities = 117/156 (75%), Positives = 127/156 (81%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           END  EV+I+ DDVVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGENPNLYYST+DV+
Sbjct: 175 ENDMPEVDISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSTDDVV 234

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLPLFHIYSLNSVLLCGLRA A IL M KFEI   LGL++KY+               
Sbjct: 235 LCVLPLFHIYSLNSVLLCGLRAGAAILMMNKFEIVSLLGLIDKYKVSIAPIVPPIVLAIA 294

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFP 492
           K P+LD YDLSSIR+LK GGAPLGKELE TVRAKFP
Sbjct: 295 KFPDLDKYDLSSIRVLKCGGAPLGKELEDTVRAKFP 330


>gb|ACI23349.1| 4-coumarate CoA ligase 2, partial [Leucaena leucocephala]
          Length = 519

 Score =  231 bits (590), Expect = 6e-71
 Identities = 117/158 (74%), Positives = 126/158 (79%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           END  EV I  DDVVALPYSSGTTGLPKGVMLTHKGL+TSIAQQVDGENPNLY+  EDVI
Sbjct: 145 ENDMPEVKIKPDDVVALPYSSGTTGLPKGVMLTHKGLLTSIAQQVDGENPNLYFHHEDVI 204

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLPLFHIYSLNSVLLCGLRAKA IL MPKFEI   LGL+ K++               
Sbjct: 205 LCVLPLFHIYSLNSVLLCGLRAKAAILLMPKFEINALLGLIQKHKVSIAPVVPPIVLAVS 264

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KS ++D YDLSSIR+ KSGGAPLGKELE +VRAKFPKA
Sbjct: 265 KSADIDKYDLSSIRVFKSGGAPLGKELEDSVRAKFPKA 302


>ref|XP_022153393.1| 4-coumarate--CoA ligase 1-like [Momordica charantia]
          Length = 465

 Score =  230 bits (586), Expect = 7e-71
 Identities = 117/158 (74%), Positives = 126/158 (79%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           E +   V+I  DDVVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDG+NPNLY+  +DVI
Sbjct: 91  EREIPAVDIGPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYFRNDDVI 150

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLPLFHIYSLNSVLLCGLRA ATIL +PKFEI   L LV KYR               
Sbjct: 151 LCVLPLFHIYSLNSVLLCGLRAGATILILPKFEIASLLQLVQKYRVSIAPVVPPIVLAIA 210

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KSP+LD YDLSSIR+LKSGGAPLGKELE TVRAKFPKA
Sbjct: 211 KSPDLDKYDLSSIRVLKSGGAPLGKELEDTVRAKFPKA 248


>gb|AHA42446.1| 4-coumarate:CoA ligase, partial [Cannabis sativa]
          Length = 391

 Score =  228 bits (580), Expect = 8e-71
 Identities = 113/156 (72%), Positives = 124/156 (79%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           END  EV I+ DDVVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGENPNLYYS  DV+
Sbjct: 179 ENDMAEVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSKNDVV 238

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLPLFHIYSLNSV+LC LRA ATIL MPKFEI   LGL+ +Y+               
Sbjct: 239 LCVLPLFHIYSLNSVMLCSLRAGATILIMPKFEIGSLLGLIERYKVSVAPIVPPIVLAIA 298

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFP 492
           K P+L  YDLSS+++LKSGGAPLGKELE TVRAKFP
Sbjct: 299 KYPDLHKYDLSSLKVLKSGGAPLGKELEDTVRAKFP 334


>gb|AFR37166.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37167.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37168.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37169.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37170.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37171.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37172.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37173.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37174.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37175.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37176.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37177.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37178.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37179.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
 gb|AFR37180.1| 4-coumarate CoA ligase, partial [Populus trichocarpa]
          Length = 169

 Score =  220 bits (560), Expect = 9e-71
 Identities = 105/158 (66%), Positives = 128/158 (81%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           EN+  +V+ + DDVVALPYSSGTTGLPKGVMLTHKGL+TS+AQQVDG+NPNLY+ +EDVI
Sbjct: 12  ENEVPQVDFSPDDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDGDNPNLYFHSEDVI 71

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLP+FHIY+LNS++LCGLR  A+IL MPKF+I   LGL+ KY+               
Sbjct: 72  LCVLPMFHIYALNSIMLCGLRVGASILIMPKFDIGTLLGLIEKYKVSIAPVVPPVMLAIA 131

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFPKA 498
           KSP+ D +DLSS+R++KSGGAPLGKELE TVRAKFP+A
Sbjct: 132 KSPDFDKHDLSSLRMIKSGGAPLGKELEDTVRAKFPQA 169


>ref|XP_009354316.1| PREDICTED: 4-coumarate--CoA ligase 1-like [Pyrus x bretschneideri]
          Length = 537

 Score =  231 bits (590), Expect = 1e-70
 Identities = 117/156 (75%), Positives = 125/156 (80%)
 Frame = +1

Query: 25  ENDFGEVNINGDDVVALPYSSGTTGLPKGVMLTHKGLVTSIAQQVDGENPNLYYSTEDVI 204
           END  EV+I  DDVVALPYSSGTTGLPKGVMLTHKGLVTS+AQQVDGENPNLYYST+DV+
Sbjct: 175 ENDMPEVDIRPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYSTDDVV 234

Query: 205 LCVLPLFHIYSLNSVLLCGLRAKATILFMPKFEIQGFLGLVNKYRXXXXXXXXXXXXXXX 384
           LCVLPLFHIYSLNSVLLCGLRA A IL M KFEI   LGL+ KY+               
Sbjct: 235 LCVLPLFHIYSLNSVLLCGLRAGAAILMMNKFEIVSLLGLIEKYKVSIAPIVPPIVLAIA 294

Query: 385 KSPELDNYDLSSIRILKSGGAPLGKELEHTVRAKFP 492
           K P+LD YDLSSIR+LK GGAPLGKELE TVRAKFP
Sbjct: 295 KFPDLDKYDLSSIRVLKCGGAPLGKELEDTVRAKFP 330