BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00005182
         (877 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_014498627.1| transcription initiation factor IIF subunit ...   467   e-165
ref|XP_017436816.1| PREDICTED: transcription initiation factor I...   465   e-164
ref|XP_007136046.1| hypothetical protein PHAVU_009G013500g [Phas...   464   e-163
ref|NP_001241605.1| uncharacterized protein LOC100788473 [Glycin...   462   e-163
ref|XP_015933899.1| transcription initiation factor IIF subunit ...   460   e-162
ref|XP_003527856.1| PREDICTED: transcription initiation factor I...   459   e-162
gb|KHN45893.1| Transcription initiation factor IIF subunit beta ...   458   e-161
gb|KHN22213.1| Transcription initiation factor IIF subunit beta ...   458   e-161
ref|XP_020219049.1| transcription initiation factor IIF subunit ...   457   e-161
gb|AFK48669.1| unknown [Lotus japonicus]                              452   e-159
ref|XP_004500963.1| PREDICTED: transcription initiation factor I...   447   e-157
ref|XP_013462187.1| transcription initiation factor IIF beta sub...   433   e-151
ref|XP_019415970.1| PREDICTED: transcription initiation factor I...   427   e-149
gb|PON52003.1| Transcription initiation factor [Parasponia ander...   426   e-148
ref|XP_018843842.1| PREDICTED: transcription initiation factor I...   424   e-148
gb|PON90993.1| Transcription initiation factor [Trema orientalis]     424   e-148
ref|XP_010105269.2| transcription initiation factor IIF subunit ...   422   e-147
ref|XP_010664754.1| PREDICTED: general transcription factor IIF ...   419   e-146
ref|XP_018821183.1| PREDICTED: transcription initiation factor I...   419   e-146
gb|OWM80888.1| hypothetical protein CDL15_Pgr006919 [Punica gran...   419   e-146

>ref|XP_014498627.1| transcription initiation factor IIF subunit beta [Vigna radiata
           var. radiata]
          Length = 261

 Score =  467 bits (1202), Expect = e-165
 Identities = 229/257 (89%), Positives = 241/257 (93%), Gaps = 1/257 (0%)
 Frame = +1

Query: 43  SGY-GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXXSA 219
           +GY GSSSSN+ETSKAERSVWLMKCP+VVAKSWQ HPPSQPL+KVVLS          SA
Sbjct: 5   NGYSGSSSSNLETSKAERSVWLMKCPLVVAKSWQTHPPSQPLAKVVLSLDPLHPEEDPSA 64

Query: 220 LQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGEN 399
           +QFTMEM  +EAVNMPK YSLNMFKDFVPMC+FSETSQGGKVAMEGKVEHKFDMKPHGEN
Sbjct: 65  VQFTMEMAGSEAVNMPKTYSLNMFKDFVPMCVFSETSQGGKVAMEGKVEHKFDMKPHGEN 124

Query: 400 IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSD 579
           IEEYGKLCRERTN+SMIKNRQIQVIDNDRGVLMRPMPGM+GL+SS+SKDKKKTQP+KQSD
Sbjct: 125 IEEYGKLCRERTNRSMIKNRQIQVIDNDRGVLMRPMPGMIGLVSSNSKDKKKTQPIKQSD 184

Query: 580 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 759
           TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT
Sbjct: 185 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 244

Query: 760 YELKPEYKKSVEDTGAE 810
           YELKPEYKKSVEDT AE
Sbjct: 245 YELKPEYKKSVEDTNAE 261


>ref|XP_017436816.1| PREDICTED: transcription initiation factor IIF subunit beta [Vigna
           angularis]
 gb|KOM51623.1| hypothetical protein LR48_Vigan09g028200 [Vigna angularis]
 dbj|BAT77726.1| hypothetical protein VIGAN_02032100 [Vigna angularis var.
           angularis]
          Length = 261

 Score =  465 bits (1196), Expect = e-164
 Identities = 228/257 (88%), Positives = 240/257 (93%), Gaps = 1/257 (0%)
 Frame = +1

Query: 43  SGY-GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXXSA 219
           +GY GSSSSN+ET KAERSVWLMKCP+VVAKSWQ HPPSQPL+KVVLS          SA
Sbjct: 5   NGYSGSSSSNLETLKAERSVWLMKCPLVVAKSWQTHPPSQPLAKVVLSLDPLHPEEDPSA 64

Query: 220 LQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGEN 399
           +QFTMEM  +EAVNMPK YSLNMFKDFVPMC+FSETSQGGKVAMEGKVEHKFDMKPHGEN
Sbjct: 65  VQFTMEMAGSEAVNMPKTYSLNMFKDFVPMCVFSETSQGGKVAMEGKVEHKFDMKPHGEN 124

Query: 400 IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSD 579
           IEEYGKLCRERTN+SMIKNRQIQVIDNDRGVLMRPMPGM+GL+SS+SKDKKKTQP+KQSD
Sbjct: 125 IEEYGKLCRERTNRSMIKNRQIQVIDNDRGVLMRPMPGMIGLVSSNSKDKKKTQPIKQSD 184

Query: 580 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 759
           TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT
Sbjct: 185 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 244

Query: 760 YELKPEYKKSVEDTGAE 810
           YELKPEYKKSVEDT AE
Sbjct: 245 YELKPEYKKSVEDTNAE 261


>ref|XP_007136046.1| hypothetical protein PHAVU_009G013500g [Phaseolus vulgaris]
 gb|ESW08040.1| hypothetical protein PHAVU_009G013500g [Phaseolus vulgaris]
          Length = 261

 Score =  464 bits (1193), Expect = e-163
 Identities = 227/257 (88%), Positives = 240/257 (93%), Gaps = 1/257 (0%)
 Frame = +1

Query: 43  SGY-GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXXSA 219
           +GY GSSSSN+ET KAERSVWLMKCP+VVAKSWQ HPPSQPL+KVVLS          SA
Sbjct: 5   NGYSGSSSSNLETLKAERSVWLMKCPLVVAKSWQTHPPSQPLAKVVLSLDPLHPEEDPSA 64

Query: 220 LQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGEN 399
           +QFTMEM  ++AVNMPK YSLNMFKDFVPMC+FSETSQGGKVAMEGKVEHKFDMKPHGEN
Sbjct: 65  VQFTMEMAGSDAVNMPKTYSLNMFKDFVPMCVFSETSQGGKVAMEGKVEHKFDMKPHGEN 124

Query: 400 IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSD 579
           IEEYGKLCRERTN+SMIKNRQIQVIDNDRGVLMRPMPGM+GL+SS+SKDKKKTQP+KQSD
Sbjct: 125 IEEYGKLCRERTNRSMIKNRQIQVIDNDRGVLMRPMPGMIGLVSSNSKDKKKTQPIKQSD 184

Query: 580 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 759
           TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT
Sbjct: 185 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 244

Query: 760 YELKPEYKKSVEDTGAE 810
           YELKPEYKKSVEDT AE
Sbjct: 245 YELKPEYKKSVEDTSAE 261


>ref|NP_001241605.1| uncharacterized protein LOC100788473 [Glycine max]
 gb|ACU23669.1| unknown [Glycine max]
 gb|KRH61088.1| hypothetical protein GLYMA_04G027700 [Glycine max]
          Length = 262

 Score =  462 bits (1189), Expect = e-163
 Identities = 230/258 (89%), Positives = 241/258 (93%), Gaps = 2/258 (0%)
 Frame = +1

Query: 43  SGY-GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXX-S 216
           +GY GSSSSN+ET+KAERSVWLMKCP+VVAKSWQ HPPSQPL+KVVLS           S
Sbjct: 5   NGYSGSSSSNLETTKAERSVWLMKCPLVVAKSWQAHPPSQPLAKVVLSLDPLHPEEDDPS 64

Query: 217 ALQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGE 396
           A+QFTMEM  +EAVNMPK YSLNMFKDFVPMC+FSETSQGGKVAMEGKVEHKFDMKPHGE
Sbjct: 65  AVQFTMEMAGSEAVNMPKTYSLNMFKDFVPMCVFSETSQGGKVAMEGKVEHKFDMKPHGE 124

Query: 397 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQS 576
           NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGM+GL+SS+SKDKKKTQPVKQS
Sbjct: 125 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMIGLVSSNSKDKKKTQPVKQS 184

Query: 577 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 756
           DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG
Sbjct: 185 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 244

Query: 757 TYELKPEYKKSVEDTGAE 810
           TYELKPEYKKSVEDT AE
Sbjct: 245 TYELKPEYKKSVEDTSAE 262


>ref|XP_015933899.1| transcription initiation factor IIF subunit beta [Arachis
           duranensis]
 ref|XP_016167582.1| transcription initiation factor IIF subunit beta [Arachis ipaensis]
          Length = 261

 Score =  460 bits (1184), Expect = e-162
 Identities = 230/258 (89%), Positives = 242/258 (93%), Gaps = 2/258 (0%)
 Frame = +1

Query: 43  SGY-GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXXSA 219
           +GY GSS+SN+ETSKAERSVWLMKCP+VV+KSWQNHPPSQPL+KVVLS          SA
Sbjct: 5   NGYVGSSTSNLETSKAERSVWLMKCPLVVSKSWQNHPPSQPLAKVVLSLDPLHADDP-SA 63

Query: 220 LQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGEN 399
           LQFTMEM  T+AVNMPK YSLNMFKDFVPMC+FSETSQGGKVAMEGKVEHKFDMKPHGEN
Sbjct: 64  LQFTMEMAGTDAVNMPKTYSLNMFKDFVPMCVFSETSQGGKVAMEGKVEHKFDMKPHGEN 123

Query: 400 IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSD 579
           IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGM+GL+SS+SKDKKKTQPVKQ+D
Sbjct: 124 IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMIGLVSSNSKDKKKTQPVKQTD 183

Query: 580 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 759
           TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT
Sbjct: 184 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 243

Query: 760 YELKPEYKKS-VEDTGAE 810
           YELKPEYKKS VEDT AE
Sbjct: 244 YELKPEYKKSVVEDTNAE 261


>ref|XP_003527856.1| PREDICTED: transcription initiation factor IIF subunit beta-like
           [Glycine max]
 gb|KRH51769.1| hypothetical protein GLYMA_06G027900 [Glycine max]
          Length = 262

 Score =  459 bits (1181), Expect = e-162
 Identities = 229/258 (88%), Positives = 239/258 (92%), Gaps = 2/258 (0%)
 Frame = +1

Query: 43  SGY-GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXX-S 216
           +GY GS SSN+ET+KAERSVWLMKCP+VVAKSWQ HPPSQPL+KVVLS           S
Sbjct: 5   NGYSGSISSNLETTKAERSVWLMKCPLVVAKSWQTHPPSQPLAKVVLSLDPLHPEEDDPS 64

Query: 217 ALQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGE 396
           A+QFTMEM  TEAVNM K YSLNMFKDFVPMC+FSETSQGGKVAMEGKVEHKFDMKPHGE
Sbjct: 65  AVQFTMEMAGTEAVNMSKTYSLNMFKDFVPMCVFSETSQGGKVAMEGKVEHKFDMKPHGE 124

Query: 397 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQS 576
           NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGM+GL+SS+SKDKKKTQPVKQS
Sbjct: 125 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMIGLVSSNSKDKKKTQPVKQS 184

Query: 577 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 756
           DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG
Sbjct: 185 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 244

Query: 757 TYELKPEYKKSVEDTGAE 810
           TYELKPEYKKSVEDT AE
Sbjct: 245 TYELKPEYKKSVEDTSAE 262


>gb|KHN45893.1| Transcription initiation factor IIF subunit beta [Glycine soja]
          Length = 262

 Score =  458 bits (1179), Expect = e-161
 Identities = 229/258 (88%), Positives = 240/258 (93%), Gaps = 2/258 (0%)
 Frame = +1

Query: 43  SGY-GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXX-S 216
           +GY GSSSSN+ET+KAERSVWLMKCP+VVAKSWQ HPPSQPL+KVVLS           S
Sbjct: 5   NGYSGSSSSNLETTKAERSVWLMKCPLVVAKSWQAHPPSQPLAKVVLSLDPLHPEEDDPS 64

Query: 217 ALQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGE 396
           A+QFTMEM  +EAVNMPK YSLNMFKDFVPMC+FSETSQGGKVAMEGKVEHKFDMKPHGE
Sbjct: 65  AVQFTMEMAGSEAVNMPKTYSLNMFKDFVPMCVFSETSQGGKVAMEGKVEHKFDMKPHGE 124

Query: 397 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQS 576
           NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGM+GL+SS+SKDKKKTQPVKQS
Sbjct: 125 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMIGLVSSNSKDKKKTQPVKQS 184

Query: 577 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 756
           DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG
Sbjct: 185 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 244

Query: 757 TYELKPEYKKSVEDTGAE 810
           TYELK EYKKSVEDT AE
Sbjct: 245 TYELKLEYKKSVEDTSAE 262


>gb|KHN22213.1| Transcription initiation factor IIF subunit beta [Glycine soja]
          Length = 262

 Score =  458 bits (1179), Expect = e-161
 Identities = 229/258 (88%), Positives = 239/258 (92%), Gaps = 2/258 (0%)
 Frame = +1

Query: 43  SGY-GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXX-S 216
           +GY GS SSN+ET+KAERSVWLMKCP+VVAKSWQ HPPSQPL+KVVLS           S
Sbjct: 5   NGYSGSISSNLETTKAERSVWLMKCPLVVAKSWQAHPPSQPLAKVVLSLDPLHPEEDDPS 64

Query: 217 ALQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGE 396
           A+QFTMEM  TEAVNM K YSLNMFKDFVPMC+FSETSQGGKVAMEGKVEHKFDMKPHGE
Sbjct: 65  AVQFTMEMAGTEAVNMSKTYSLNMFKDFVPMCVFSETSQGGKVAMEGKVEHKFDMKPHGE 124

Query: 397 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQS 576
           NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGM+GL+SS+SKDKKKTQPVKQS
Sbjct: 125 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMIGLVSSNSKDKKKTQPVKQS 184

Query: 577 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 756
           DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG
Sbjct: 185 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 244

Query: 757 TYELKPEYKKSVEDTGAE 810
           TYELKPEYKKSVEDT AE
Sbjct: 245 TYELKPEYKKSVEDTSAE 262


>ref|XP_020219049.1| transcription initiation factor IIF subunit beta [Cajanus cajan]
          Length = 262

 Score =  457 bits (1177), Expect = e-161
 Identities = 227/258 (87%), Positives = 240/258 (93%), Gaps = 2/258 (0%)
 Frame = +1

Query: 43  SGY-GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXX-S 216
           +GY GSSSSN+ET+KAERSVWLMKCP+VVAKSWQ HPPSQPL+KVVLS           S
Sbjct: 5   NGYSGSSSSNLETTKAERSVWLMKCPLVVAKSWQTHPPSQPLAKVVLSLDPLHPEEDDPS 64

Query: 217 ALQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGE 396
           A+QFTMEM  ++AVNM K YSLNMFKDFVPMC+FSETSQGGKVAMEGKVEHKFDMKPHGE
Sbjct: 65  AVQFTMEMAGSDAVNMSKTYSLNMFKDFVPMCVFSETSQGGKVAMEGKVEHKFDMKPHGE 124

Query: 397 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQS 576
           NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGM+GL+SS++KDKKKTQPVKQS
Sbjct: 125 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMIGLVSSNAKDKKKTQPVKQS 184

Query: 577 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 756
           DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG
Sbjct: 185 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 244

Query: 757 TYELKPEYKKSVEDTGAE 810
           TYELKPEYKKSVEDT AE
Sbjct: 245 TYELKPEYKKSVEDTSAE 262


>gb|AFK48669.1| unknown [Lotus japonicus]
          Length = 258

 Score =  452 bits (1162), Expect = e-159
 Identities = 225/253 (88%), Positives = 232/253 (91%), Gaps = 2/253 (0%)
 Frame = +1

Query: 58  SSSNVETSKAERSVWLMKCPVVVAKSWQNH--PPSQPLSKVVLSXXXXXXXXXXSALQFT 231
           S SNVETSKAERSVWLMKCPVVVAKSWQNH   PSQPLSKVVLS          S LQFT
Sbjct: 6   SVSNVETSKAERSVWLMKCPVVVAKSWQNHHPSPSQPLSKVVLSLDPLLPEDDPSHLQFT 65

Query: 232 MEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGENIEEY 411
           MEM  +EAVNMPK Y+LNMFKDFVPMC+FSETSQGGKVAMEGKVEHKFDMKPHGENIEEY
Sbjct: 66  MEMTGSEAVNMPKTYALNMFKDFVPMCVFSETSQGGKVAMEGKVEHKFDMKPHGENIEEY 125

Query: 412 GKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSDTKRT 591
           GKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGM+GL+SS+SKDKK+TQPVKQSDTKRT
Sbjct: 126 GKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMIGLVSSNSKDKKRTQPVKQSDTKRT 185

Query: 592 RRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGTYELK 771
           RRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGTYELK
Sbjct: 186 RRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGTYELK 245

Query: 772 PEYKKSVEDTGAE 810
           PEYKKSVED   E
Sbjct: 246 PEYKKSVEDVKVE 258


>ref|XP_004500963.1| PREDICTED: transcription initiation factor IIF subunit beta [Cicer
           arietinum]
          Length = 261

 Score =  447 bits (1149), Expect = e-157
 Identities = 223/257 (86%), Positives = 232/257 (90%), Gaps = 1/257 (0%)
 Frame = +1

Query: 43  SGYGSSSS-NVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXXSA 219
           +GYG SS  N+ETSKAERSVWLMKCPV VAKSWQNHPPSQPLSKVV S          S 
Sbjct: 5   NGYGGSSGPNLETSKAERSVWLMKCPVAVAKSWQNHPPSQPLSKVVFSINPLLPEDDPSH 64

Query: 220 LQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGEN 399
           LQFTMEM  TE+VNMPK YSLNMFKDFVPMCIFSETS+G  VAMEGKVEHKFDMKP  EN
Sbjct: 65  LQFTMEMSGTESVNMPKTYSLNMFKDFVPMCIFSETSEGDSVAMEGKVEHKFDMKPRHEN 124

Query: 400 IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSD 579
           IEEYGKLCRERTNKS IKNRQIQVI+ND G+LMRPMPGM+GLISSSSKDKK+TQPVKQ+D
Sbjct: 125 IEEYGKLCRERTNKSSIKNRQIQVIENDCGLLMRPMPGMLGLISSSSKDKKRTQPVKQTD 184

Query: 580 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 759
           TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT
Sbjct: 185 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 244

Query: 760 YELKPEYKKSVEDTGAE 810
           YELKPEYKKSVEDTGAE
Sbjct: 245 YELKPEYKKSVEDTGAE 261


>ref|XP_013462187.1| transcription initiation factor IIF beta subunit [Medicago
           truncatula]
 gb|ACJ85879.1| unknown [Medicago truncatula]
 gb|AFK34287.1| unknown [Medicago truncatula]
 gb|KEH36222.1| transcription initiation factor IIF beta subunit [Medicago
           truncatula]
          Length = 262

 Score =  433 bits (1113), Expect = e-151
 Identities = 214/258 (82%), Positives = 228/258 (88%), Gaps = 2/258 (0%)
 Frame = +1

Query: 43  SGYGSSS--SNVETSKAERSVWLMKCPVVVAKSWQNHPPSQPLSKVVLSXXXXXXXXXXS 216
           + YG SS  SN+ETSKAERSVWLMKCPV VAKSWQNHPPSQPLSKVV S          +
Sbjct: 5   NSYGGSSGGSNLETSKAERSVWLMKCPVAVAKSWQNHPPSQPLSKVVFSIDPLLPEDDPA 64

Query: 217 ALQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGE 396
            LQFTMEM  TEAVNMPK YSLNMFKDFVPMCIFSETS+G KVAMEGKVEHKFDMKP  E
Sbjct: 65  HLQFTMEMSGTEAVNMPKTYSLNMFKDFVPMCIFSETSEGDKVAMEGKVEHKFDMKPRHE 124

Query: 397 NIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQS 576
           N+++YGKLCRERT KSMIKNRQ+Q+I +DRG  MRPMPGMVGL+SS+ KDKK+TQPVKQ+
Sbjct: 125 NMDDYGKLCRERTKKSMIKNRQVQIIADDRGTHMRPMPGMVGLVSSNFKDKKRTQPVKQT 184

Query: 577 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 756
           DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG
Sbjct: 185 DTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQG 244

Query: 757 TYELKPEYKKSVEDTGAE 810
           TYELKPEYKKSVED  AE
Sbjct: 245 TYELKPEYKKSVEDANAE 262


>ref|XP_019415970.1| PREDICTED: transcription initiation factor IIF subunit beta
           [Lupinus angustifolius]
 gb|OIV96322.1| hypothetical protein TanjilG_09749 [Lupinus angustifolius]
          Length = 264

 Score =  427 bits (1098), Expect = e-149
 Identities = 217/261 (83%), Positives = 230/261 (88%), Gaps = 9/261 (3%)
 Frame = +1

Query: 55  SSSSNVETSKAERSVWLMKCPVVVAKSWQN---------HPPSQPLSKVVLSXXXXXXXX 207
           + SSN+ETSKAERSVWLMKCPVVVAKSWQ          H    PL+KVVLS        
Sbjct: 5   NGSSNLETSKAERSVWLMKCPVVVAKSWQQNHNHNNNNQHHSQPPLAKVVLSLDPLQPDD 64

Query: 208 XXSALQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKP 387
             S+LQFTMEM  + AVNMPK+Y+LNMFKDFVPM +FSET+QGG VAMEGKVEHKFDMKP
Sbjct: 65  P-SSLQFTMEMAGSGAVNMPKSYALNMFKDFVPMGVFSETNQGGNVAMEGKVEHKFDMKP 123

Query: 388 HGENIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPV 567
           HGE+IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISS+SKDKK+TQP+
Sbjct: 124 HGEDIEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSNSKDKKRTQPL 183

Query: 568 KQSDTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGA 747
           KQSDTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGA
Sbjct: 184 KQSDTKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGA 243

Query: 748 NQGTYELKPEYKKSVEDTGAE 810
           NQGTYELKPEYKKSVEDT AE
Sbjct: 244 NQGTYELKPEYKKSVEDTSAE 264


>gb|PON52003.1| Transcription initiation factor [Parasponia andersonii]
          Length = 260

 Score =  426 bits (1095), Expect = e-148
 Identities = 214/257 (83%), Positives = 226/257 (87%), Gaps = 2/257 (0%)
 Frame = +1

Query: 46  GYGSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQP--LSKVVLSXXXXXXXXXXSA 219
           G G SSSN+ET K+ERSVWLMKCP+VVAKSWQ+HPPS P  L+KVVLS          S 
Sbjct: 5   GSGGSSSNLETGKSERSVWLMKCPLVVAKSWQSHPPSDPHPLAKVVLSLDPLHSDDSNS- 63

Query: 220 LQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGEN 399
           LQFTMEM   E  N PK+YSLNMFKDFVPM +FSETSQGGKVA+EGKVEHKFDMKPHGEN
Sbjct: 64  LQFTMEMAGAETGNKPKSYSLNMFKDFVPMSVFSETSQGGKVAVEGKVEHKFDMKPHGEN 123

Query: 400 IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSD 579
           +E+YGKLCRERT KSMIKNR IQVIDNDRGV MRPMPGM+GLISSSSKDKKKT PVKQSD
Sbjct: 124 LEDYGKLCRERTKKSMIKNRTIQVIDNDRGVHMRPMPGMLGLISSSSKDKKKTAPVKQSD 183

Query: 580 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 759
            KRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRG NQGT
Sbjct: 184 VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGT 243

Query: 760 YELKPEYKKSVEDTGAE 810
           YELKPEYKKSVED+ AE
Sbjct: 244 YELKPEYKKSVEDSNAE 260


>ref|XP_018843842.1| PREDICTED: transcription initiation factor IIF subunit beta-like
           [Juglans regia]
          Length = 263

 Score =  424 bits (1091), Expect = e-148
 Identities = 217/255 (85%), Positives = 231/255 (90%), Gaps = 2/255 (0%)
 Frame = +1

Query: 52  GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQP--LSKVVLSXXXXXXXXXXSALQ 225
           GSSSSNV+T+KAERSVWLMKCPVVVAKSWQ+HP S P  L+KVVLS          S+LQ
Sbjct: 11  GSSSSNVDTAKAERSVWLMKCPVVVAKSWQSHPHSDPHPLAKVVLSLDPLRHDDS-SSLQ 69

Query: 226 FTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGENIE 405
           FTMEM  TEA ++PK+YSLNMFKDFVPMCIFSET+QG KVAMEGKVEHKFDMKPHGENIE
Sbjct: 70  FTMEMAGTEAGSIPKSYSLNMFKDFVPMCIFSETNQG-KVAMEGKVEHKFDMKPHGENIE 128

Query: 406 EYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSDTK 585
           EYG+LCRERT+KSMIKNRQI+VIDNDRGV MRPMPGMVGLISS+SKDKKKT PVKQSD K
Sbjct: 129 EYGRLCRERTSKSMIKNRQIRVIDNDRGVHMRPMPGMVGLISSTSKDKKKTAPVKQSDVK 188

Query: 586 RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGTYE 765
           RTRRDRGELEDIMFKLFERQ NWALKQLVQETDQPAQFLKEILNELCVYNKRG NQGTYE
Sbjct: 189 RTRRDRGELEDIMFKLFERQSNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGTYE 248

Query: 766 LKPEYKKSVEDTGAE 810
           LKPEYKK+VEDT AE
Sbjct: 249 LKPEYKKAVEDTTAE 263


>gb|PON90993.1| Transcription initiation factor [Trema orientalis]
          Length = 260

 Score =  424 bits (1089), Expect = e-148
 Identities = 213/257 (82%), Positives = 225/257 (87%), Gaps = 2/257 (0%)
 Frame = +1

Query: 46  GYGSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQP--LSKVVLSXXXXXXXXXXSA 219
           G   SSSN+ET K+ERSVWLMKCP+VVAKSWQ+HPPS P  L+KVVLS          S 
Sbjct: 5   GSSGSSSNLETGKSERSVWLMKCPLVVAKSWQSHPPSDPHPLAKVVLSLDPLHSDDSNS- 63

Query: 220 LQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGEN 399
           LQFTMEM   E  N PK+YSLNMFKDFVPM +FSETSQGGKVA+EGKVEHKFDMKPHGEN
Sbjct: 64  LQFTMEMAGAETGNKPKSYSLNMFKDFVPMSVFSETSQGGKVAVEGKVEHKFDMKPHGEN 123

Query: 400 IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSD 579
           +E+YGKLCRERT KSMIKNR IQVIDNDRGV MRPMPGM+GLISSSSKDKKKT PVKQSD
Sbjct: 124 LEDYGKLCRERTKKSMIKNRTIQVIDNDRGVHMRPMPGMLGLISSSSKDKKKTAPVKQSD 183

Query: 580 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 759
            KRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRG NQGT
Sbjct: 184 VKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGT 243

Query: 760 YELKPEYKKSVEDTGAE 810
           YELKPEYKKSVED+ AE
Sbjct: 244 YELKPEYKKSVEDSNAE 260


>ref|XP_010105269.2| transcription initiation factor IIF subunit beta [Morus notabilis]
          Length = 259

 Score =  422 bits (1086), Expect = e-147
 Identities = 215/257 (83%), Positives = 226/257 (87%), Gaps = 2/257 (0%)
 Frame = +1

Query: 46  GYGSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQP--LSKVVLSXXXXXXXXXXSA 219
           G   SSSN+ET KAERSVWLMKCP+VVAKSWQ+H PS P  L+KVVLS          S+
Sbjct: 5   GGSGSSSNLETGKAERSVWLMKCPLVVAKSWQSHSPSDPHPLAKVVLSLDPLLSDDP-SS 63

Query: 220 LQFTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGEN 399
           LQFTME   TE  + PK YSLNMFKDFVPMC+FSET+QG KVA+EGKVEHKFDMKPHGEN
Sbjct: 64  LQFTMETAATETGHKPKTYSLNMFKDFVPMCVFSETNQG-KVAVEGKVEHKFDMKPHGEN 122

Query: 400 IEEYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSD 579
           +EEYGKLCRERTNKSMIKNRQIQVIDNDRGV MRPMPGMVGLISSSSKDKKKT PVKQSD
Sbjct: 123 LEEYGKLCRERTNKSMIKNRQIQVIDNDRGVHMRPMPGMVGLISSSSKDKKKTAPVKQSD 182

Query: 580 TKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGT 759
            KRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRG NQGT
Sbjct: 183 MKRTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGT 242

Query: 760 YELKPEYKKSVEDTGAE 810
           YELKPEYKKSVEDT A+
Sbjct: 243 YELKPEYKKSVEDTNAD 259


>ref|XP_010664754.1| PREDICTED: general transcription factor IIF subunit 2 isoform X2
           [Vitis vinifera]
 emb|CBI19782.3| unnamed protein product, partial [Vitis vinifera]
          Length = 259

 Score =  419 bits (1078), Expect = e-146
 Identities = 213/254 (83%), Positives = 226/254 (88%), Gaps = 2/254 (0%)
 Frame = +1

Query: 55  SSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPS--QPLSKVVLSXXXXXXXXXXSALQF 228
           SSSSN+ET KAERSVWLMKCP+ V+KSWQ+H  S  QP++KVVLS          SAL+F
Sbjct: 8   SSSSNLETGKAERSVWLMKCPLAVSKSWQSHSSSESQPVAKVVLSLDPLRSEDP-SALEF 66

Query: 229 TMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGENIEE 408
           TMEM  T A NMPK+YSLNMFKDFVPMC+FSET+QG +VAMEGKVEHKFDMKPH ENIEE
Sbjct: 67  TMEMTGTGAPNMPKSYSLNMFKDFVPMCVFSETNQG-RVAMEGKVEHKFDMKPHNENIEE 125

Query: 409 YGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSDTKR 588
           YGKLCRERTNKSMIKNRQIQVIDNDRGV MRPMPGMVGLI+S+SKDKKKT PVK SD KR
Sbjct: 126 YGKLCRERTNKSMIKNRQIQVIDNDRGVHMRPMPGMVGLIASNSKDKKKTAPVKGSDMKR 185

Query: 589 TRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGTYEL 768
           TRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRG NQGTYEL
Sbjct: 186 TRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGTYEL 245

Query: 769 KPEYKKSVEDTGAE 810
           KPEYKKS EDTGAE
Sbjct: 246 KPEYKKSAEDTGAE 259


>ref|XP_018821183.1| PREDICTED: transcription initiation factor IIF subunit beta-like
           [Juglans regia]
          Length = 262

 Score =  419 bits (1078), Expect = e-146
 Identities = 214/255 (83%), Positives = 229/255 (89%), Gaps = 2/255 (0%)
 Frame = +1

Query: 52  GSSSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQP--LSKVVLSXXXXXXXXXXSALQ 225
           GSSSSN+ET+KAERSVWLMKCPV VAKSW++HP S P  L+KVVLS          S+LQ
Sbjct: 10  GSSSSNLETAKAERSVWLMKCPVAVAKSWKSHPQSDPHPLAKVVLSLDPLHPDGS-SSLQ 68

Query: 226 FTMEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGENIE 405
           FTMEM  TEA ++PK+YSLNMFKDFVPM +FSET+QG KVAMEGKVEHKFDMKPHGENIE
Sbjct: 69  FTMEMAGTEAGSVPKSYSLNMFKDFVPMSVFSETNQG-KVAMEGKVEHKFDMKPHGENIE 127

Query: 406 EYGKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSDTK 585
           EYGKLCRERT+KSMIK+RQIQVIDNDRG+ MRPMPGMVGLISS+SKDKKKT PVKQSD K
Sbjct: 128 EYGKLCRERTSKSMIKSRQIQVIDNDRGIHMRPMPGMVGLISSTSKDKKKTVPVKQSDVK 187

Query: 586 RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGTYE 765
           RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRG NQGTYE
Sbjct: 188 RTRRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGTNQGTYE 247

Query: 766 LKPEYKKSVEDTGAE 810
           LKPEYKK VEDT AE
Sbjct: 248 LKPEYKKVVEDTTAE 262


>gb|OWM80888.1| hypothetical protein CDL15_Pgr006919 [Punica granatum]
 gb|PKI45711.1| hypothetical protein CRG98_034027 [Punica granatum]
          Length = 258

 Score =  419 bits (1077), Expect = e-146
 Identities = 209/253 (82%), Positives = 227/253 (89%), Gaps = 2/253 (0%)
 Frame = +1

Query: 58  SSSNVETSKAERSVWLMKCPVVVAKSWQNHPPSQP--LSKVVLSXXXXXXXXXXSALQFT 231
           SSS VET+KA+RSVWLMKCPVVVAKSWQ+HPPS P  ++KVVLS          SALQFT
Sbjct: 7   SSSRVETAKADRSVWLMKCPVVVAKSWQSHPPSDPTPVAKVVLSLDPLRPPDDPSALQFT 66

Query: 232 MEMVRTEAVNMPKAYSLNMFKDFVPMCIFSETSQGGKVAMEGKVEHKFDMKPHGENIEEY 411
           MEM   E  ++P +YSLNM KDFVPMCIFSETSQG KVA EGKVEHKFDMKPH +NIEEY
Sbjct: 67  MEMAGAEPGHVPNSYSLNMSKDFVPMCIFSETSQG-KVAAEGKVEHKFDMKPHSDNIEEY 125

Query: 412 GKLCRERTNKSMIKNRQIQVIDNDRGVLMRPMPGMVGLISSSSKDKKKTQPVKQSDTKRT 591
           G+LCRERT+KSM+KNRQIQVIDNDRGV MRPMPGM+G+ISS++KDKKKTQPVKQSD KRT
Sbjct: 126 GRLCRERTSKSMVKNRQIQVIDNDRGVHMRPMPGMLGIISSTTKDKKKTQPVKQSDMKRT 185

Query: 592 RRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFLKEILNELCVYNKRGANQGTYELK 771
           RRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQF+KEILN+LCVYNKRGANQGTYELK
Sbjct: 186 RRDRGELEDIMFKLFERQPNWALKQLVQETDQPAQFVKEILNDLCVYNKRGANQGTYELK 245

Query: 772 PEYKKSVEDTGAE 810
           PEYKKSVEDTGAE
Sbjct: 246 PEYKKSVEDTGAE 258