BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00004262
         (1278 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arieti...   253   7e-71
sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName:...   237   2e-65
dbj|GAU12625.1| hypothetical protein TSUD_121160 [Trifolium subt...   222   4e-60
dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subt...   222   4e-60
dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subt...   220   1e-59
ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago tru...   218   9e-59
ref|XP_017425524.1| PREDICTED: DNA topoisomerase 2 [Vigna angula...   216   4e-58
gb|KHN45085.1| DNA topoisomerase 2, partial [Glycine soja]            213   5e-57
ref|XP_003545986.1| PREDICTED: DNA topoisomerase 2-like [Glycine...   210   6e-56
ref|XP_016170361.1| DNA topoisomerase 2 isoform X2 [Arachis ipae...   210   6e-56
ref|XP_016170360.1| DNA topoisomerase 2 isoform X1 [Arachis ipae...   210   6e-56
ref|XP_014501355.1| DNA topoisomerase 2 [Vigna radiata var. radi...   208   3e-55
gb|KRH32967.1| hypothetical protein GLYMA_10G090000 [Glycine max]     203   1e-53
ref|XP_014618555.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisom...   203   1e-53
ref|XP_015936924.1| DNA topoisomerase 2 isoform X2 [Arachis dura...   201   5e-53
ref|XP_015936922.1| DNA topoisomerase 2 isoform X1 [Arachis dura...   201   5e-53
gb|PNY06816.1| DNA topoisomerase 2-like protein [Trifolium prate...   195   6e-51
ref|XP_007148908.1| hypothetical protein PHAVU_005G024200g [Phas...   195   6e-51
dbj|GAU30192.1| hypothetical protein TSUD_311430 [Trifolium subt...   192   7e-50
ref|XP_015952953.1| DNA topoisomerase 2-like [Arachis duranensis]     191   2e-49

>ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
 ref|XP_004517303.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
          Length = 1472

 Score =  253 bits (646), Expect = 7e-71
 Identities = 198/487 (40%), Positives = 244/487 (50%), Gaps = 70/487 (14%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYYEKR++ IL+NL+RLL +L N V+FIL  +  ++   +  + DLL+ELK++GFTPM
Sbjct: 1003 RLEYYEKRKKYILANLERLLLILDNKVRFILGVVSGEIIVSNRKKADLLIELKKKGFTPM 1062

Query: 178  PREGKSTS---TGANDDNLEALKD-----------------DYEYLLYLPYKAFVIEEVQ 297
            PR+GKS      GA DDN E  +D                 DYEYLL LP     +E VQ
Sbjct: 1063 PRKGKSAEPQVAGAIDDNSEEQEDEQDTVSRPVSVEGATWGDYEYLLSLPIGTLTLESVQ 1122

Query: 298  ELLAEMNEKKKQFDVLKTTTAKSLWMKD-------LNELEKKLDE--------------- 411
            +L  E  EK+K+F++L  T   SLWMKD       L+EL+ K  E               
Sbjct: 1123 KLRDEKAEKEKEFEILMGTPTTSLWMKDLDEFETKLDELDNKDAEDERKRSSQASKKAPN 1182

Query: 412  --IYGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNVVKVTKPKGIAGCMKIQKDSAATD 585
                                               N V+V KPKG A     QK++   D
Sbjct: 1183 GFASKAAKKPPQPRKNTKKAKTVEPENDNSSMEIENPVEVAKPKGRAVSKNTQKET--DD 1240

Query: 586  AILSLQEFQGLDAYNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGA 765
             ILSLQE                                      L AYN   SGEK+ A
Sbjct: 1241 DILSLQE-------------------------------------RLAAYNFESSGEKSAA 1263

Query: 766  LETEEPPLPAGKKQNNRKGGAKK--TTVVLD-TDSENEVGXXXXXXXFELVQQ--PAPEA 930
            +ETEEP +PA KK  NR+GG KK  +T+VLD +DS+N+V        FE+ Q+  PAPEA
Sbjct: 1264 METEEPAVPAAKKPINRRGGVKKKSSTIVLDESDSDNDVNNIDDDDDFEVQQKAAPAPEA 1323

Query: 931  LKKEGG--------------------RNLGTKRTFGQMLLTDMLQPAEGTGTSSPEKKVR 1050
             KK+GG                    RN+G K+T GQ LLTDMLQPAEGTGT SPEKKVR
Sbjct: 1324 GKKKGGGRKPAAQNTKKAAAPAAPKKRNVGGKQTMGQKLLTDMLQPAEGTGT-SPEKKVR 1382

Query: 1051 KMRESPFNKGKSPSEDLSWGSGSAKSGSILGRAANRDKSQSEDLSLGSGSATRFPLNFEE 1230
            KMRESPFNK               KSGSILGRAAN+D S   DLS  +GSA+  P   ++
Sbjct: 1383 KMRESPFNK---------------KSGSILGRAANKDISPIADLS--AGSASNSPA--DD 1423

Query: 1231 DVAEIAP 1251
            DV EIAP
Sbjct: 1424 DVVEIAP 1430


>sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase
            II; AltName: Full=PsTopII
 emb|CAA74891.1| topoisomerase II [Pisum sativum]
          Length = 1462

 Score =  237 bits (605), Expect = 2e-65
 Identities = 190/474 (40%), Positives = 241/474 (50%), Gaps = 57/474 (12%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RL+YYEK +E IL NL RLL +L N V+FIL  +  ++   +  + +LL+ELK +GFTPM
Sbjct: 982  RLDYYEKSKEYILGNLNRLLLILDNKVRFILGVVNGEIIVSNRKKAELLIELKEKGFTPM 1041

Query: 178  PREGKSTS---TGANDDNLEALKD------------------DYEYLLYLPYKAFVIEEV 294
            PR+GKST     GANDD+ E  +D                  DY+ LL LP     +E V
Sbjct: 1042 PRKGKSTKPQVAGANDDDSEEQEDAEPETASQSVSVEGATWGDYDDLLSLPIGTLTLESV 1101

Query: 295  QELLAEMNEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEI---YGXXXXXXXXXXXXXXX 465
            Q+LL E  EK+K++++L  T   SLW+KDL+E EKKLDE+   Y                
Sbjct: 1102 QKLLDEKTEKEKEYEILSGTPTTSLWLKDLDEFEKKLDELDLKYAEDDRKRASQGSKKAN 1161

Query: 466  XXXXXXXXXXXXXXXNVVKV--TKPKGIAGCMKIQKDSAATDAILSLQEFQGLDAYNFKP 639
                           N  K    +P+     M+I+    A              A   KP
Sbjct: 1162 GFASKPAKKPPQPRKNTKKAKSVEPENDNSSMEIENAVEAAKP-----------AEVAKP 1210

Query: 640  SGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGALETEE-PPLPAGKKQNNR 816
             G    A                 +E L AYN+  SGEK+ A+E+EE     AGKKQNN+
Sbjct: 1211 KGR---AAPKKNIQKEPEDDIQSLQERLAAYNIESSGEKSQAMESEEVQQKAAGKKQNNK 1267

Query: 817  KGGAKK--TTVVLDTDSENEVGXXXXXXX-FELVQQPAPEALKKEGGR------------ 951
            +GGAKK  +T+VL++DS+NEV         FE VQQ A  A  K+GGR            
Sbjct: 1268 RGGAKKKSSTIVLESDSDNEVNDVDDDDDDFEEVQQKA--APVKKGGRKPAAQNAKKAPA 1325

Query: 952  -------------NLGTKRTFGQMLLTDMLQPAEGTGTSSPEKKVRKMRESPFNKGKSPS 1092
                         ++GTK++ GQ LLTDMLQPAEGTGT SPEKKVRKMRESPFNK     
Sbjct: 1326 KAPAKAPAAPKKRSVGTKQSAGQKLLTDMLQPAEGTGT-SPEKKVRKMRESPFNK----- 1379

Query: 1093 EDLSWGSGSAKSGSILGR-AANRDKSQSEDLSLGSGSATRFPLNFEEDVAEIAP 1251
                      KSGSILGR AA +D S   D S GS S T  PL+ E++V EIAP
Sbjct: 1380 ----------KSGSILGRAAAAKDISPIADCSAGSASNT--PLS-EDEVVEIAP 1420


>dbj|GAU12625.1| hypothetical protein TSUD_121160 [Trifolium subterraneum]
          Length = 1315

 Score =  222 bits (565), Expect = 4e-60
 Identities = 185/505 (36%), Positives = 252/505 (49%), Gaps = 79/505 (15%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYYEKR++ IL+NL+RLL +L N V+FIL  +  ++   +  + +LL ELK++GFTPM
Sbjct: 817  RLEYYEKRKKYILANLERLLLILDNKVRFILGVVNGEIIVSNRKKAELLKELKQKGFTPM 876

Query: 178  PREGKSTS---TGANDDNLEALKD------------------DYEYLLYLPYKAFVIEEV 294
            PR+GKS+     GAND++ E  +D                  DYEYLL L      +E V
Sbjct: 877  PRKGKSSEPQVAGANDESSEEREDIEQETGSQPVRVEEATWGDYEYLLSLSIGTLTLESV 936

Query: 295  QELLAEMNEKKKQFDVLKTTTAKSLWM-------KDLNELE-KKLDEIYGXXXXXXXXXX 450
            Q+LL E  EK+K+F++L  T +K+LW+       K L+EL+ K+ +E             
Sbjct: 937  QKLLNEKAEKEKEFEILMGTPSKALWLKDLDEFEKKLDELDSKEAEEGRKRSSQASKKNG 996

Query: 451  XXXXXXXXXXXXXXXXXXXXNVVKVTKPKGIAGCMKIQK--DSAATDAILSLQEFQGLDA 624
                                N+    +P+     M+I+      A     + ++ Q ++ 
Sbjct: 997  FASKPAKKPPQPRKNTKKAQNI----EPENDNSPMEIENAVGKKAPQPRKNTKKTQNVEP 1052

Query: 625  YNFKPSGE----------KTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGALET 774
             N+  S E          K  A                 +E L AYN+  SGEK+ A+E+
Sbjct: 1053 ENYNSSMEIENAVDIAKPKGRAGSKKNTPKEPEEEILSLQERLAAYNIESSGEKSQAMES 1112

Query: 775  EEPPLPAGKKQNNRKGGAKK--TTVVLDTDSENEVGXXXXXXXFELVQQPAPEALKKEGG 948
            EEP +P  KKQ NR+GGAKK   T+VL++DS+NEV        FE+ ++ AP    K+GG
Sbjct: 1113 EEP-VPDMKKQINRRGGAKKKSNTIVLESDSDNEVNDVDDDDDFEVQEKAAPV---KKGG 1168

Query: 949  R---------------------NLGTKRTFGQMLLTDMLQPAEGTGTSSPEKKVRKMRES 1065
            R                     N+GTK+  GQ LLTDMLQPAE TGT SPEKKVRKMRES
Sbjct: 1169 RKPAAQNAKKAPAKAPAAPKKRNVGTKQLSGQKLLTDMLQPAESTGT-SPEKKVRKMRES 1227

Query: 1066 PFNKGKSPSEDLSWGSGSAKSGSILGRAANRDKSQSEDLSLGSGSATRFPLNFEEDVAEI 1245
            PFNK               KSGS+LGR AN+D S   D S   GS +  P+  EE+V EI
Sbjct: 1228 PFNK---------------KSGSVLGRDANKDISPLADFS--EGSTSNSPI-AEEEVVEI 1269

Query: 1246 ------APPE--------YIGSESE 1278
                  A P+        Y+ SESE
Sbjct: 1270 PSQPSRARPQRANRTQKKYVASESE 1294


>dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subterraneum]
          Length = 1359

 Score =  222 bits (565), Expect = 4e-60
 Identities = 185/505 (36%), Positives = 252/505 (49%), Gaps = 79/505 (15%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYYEKR++ IL+NL+RLL +L N V+FIL  +  ++   +  + +LL ELK++GFTPM
Sbjct: 861  RLEYYEKRKKYILANLERLLLILDNKVRFILGVVNGEIIVSNRKKAELLKELKQKGFTPM 920

Query: 178  PREGKSTS---TGANDDNLEALKD------------------DYEYLLYLPYKAFVIEEV 294
            PR+GKS+     GAND++ E  +D                  DYEYLL L      +E V
Sbjct: 921  PRKGKSSEPQVAGANDESSEEREDIEQETGSQPVRVEEATWGDYEYLLSLSIGTLTLESV 980

Query: 295  QELLAEMNEKKKQFDVLKTTTAKSLWM-------KDLNELE-KKLDEIYGXXXXXXXXXX 450
            Q+LL E  EK+K+F++L  T +K+LW+       K L+EL+ K+ +E             
Sbjct: 981  QKLLNEKAEKEKEFEILMGTPSKALWLKDLDEFEKKLDELDSKEAEEGRKRSSQASKKNG 1040

Query: 451  XXXXXXXXXXXXXXXXXXXXNVVKVTKPKGIAGCMKIQK--DSAATDAILSLQEFQGLDA 624
                                N+    +P+     M+I+      A     + ++ Q ++ 
Sbjct: 1041 FASKPAKKPPQPRKNTKKAQNI----EPENDNSPMEIENAVGKKAPQPRKNTKKTQNVEP 1096

Query: 625  YNFKPSGE----------KTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGALET 774
             N+  S E          K  A                 +E L AYN+  SGEK+ A+E+
Sbjct: 1097 ENYNSSMEIENAVDIAKPKGRAGSKKNTPKEPEEEILSLQERLAAYNIESSGEKSQAMES 1156

Query: 775  EEPPLPAGKKQNNRKGGAKK--TTVVLDTDSENEVGXXXXXXXFELVQQPAPEALKKEGG 948
            EEP +P  KKQ NR+GGAKK   T+VL++DS+NEV        FE+ ++ AP    K+GG
Sbjct: 1157 EEP-VPDMKKQINRRGGAKKKSNTIVLESDSDNEVNDVDDDDDFEVQEKAAPV---KKGG 1212

Query: 949  R---------------------NLGTKRTFGQMLLTDMLQPAEGTGTSSPEKKVRKMRES 1065
            R                     N+GTK+  GQ LLTDMLQPAE TGT SPEKKVRKMRES
Sbjct: 1213 RKPAAQNAKKAPAKAPAAPKKRNVGTKQLSGQKLLTDMLQPAESTGT-SPEKKVRKMRES 1271

Query: 1066 PFNKGKSPSEDLSWGSGSAKSGSILGRAANRDKSQSEDLSLGSGSATRFPLNFEEDVAEI 1245
            PFNK               KSGS+LGR AN+D S   D S   GS +  P+  EE+V EI
Sbjct: 1272 PFNK---------------KSGSVLGRDANKDISPLADFS--EGSTSNSPI-AEEEVVEI 1313

Query: 1246 ------APPE--------YIGSESE 1278
                  A P+        Y+ SESE
Sbjct: 1314 PSQPSRARPQRANRTQKKYVASESE 1338


>dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subterraneum]
          Length = 1336

 Score =  220 bits (561), Expect = 1e-59
 Identities = 175/450 (38%), Positives = 226/450 (50%), Gaps = 77/450 (17%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            R+EYYE+R++ IL+NL+RLL +L N V+FIL  +  ++   +  + +LL+ELK++GFTPM
Sbjct: 851  RMEYYERRKKYILANLERLLLILDNKVRFILGVVNGEIIVSNRKKAELLIELKQKGFTPM 910

Query: 178  PREGKSTS---TGANDDNLEALKD------------------DYEYLLYLPYKAFVIEEV 294
             R+GKS      GANDD+ E  +D                  DYEYLL LP     +E V
Sbjct: 911  -RKGKSAEPQVAGANDDSSEGQEDGEQETVSQPVNVEGATWGDYEYLLSLPIGTLTLESV 969

Query: 295  QELLAEMNEKKKQFDVLKTTTAKSLWMKDL-------NELEKKLDE-------------- 411
            Q+LL E  EK+K+ ++L  T  KSLW+KDL       +EL+ K  E              
Sbjct: 970  QKLLDEKAEKEKEHEILMGTPTKSLWLKDLDDFEKKLDELDSKEAEDDRKRSSQASKKTA 1029

Query: 412  ---IYGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNVVKVTKPKGIAGCMKIQKDSAAT 582
                                                NVV+V KPKG AG    QK +   
Sbjct: 1030 NGFAAKPAKKPPQPRKNTKKNKNVEPENDNSSTEVENVVEVVKPKGRAGSKNTQKKAGDE 1089

Query: 583  DAILSLQEFQGLDAYNFKPSGEKTG-------------AXXXXXXXXXXXXXXXXYRELL 723
              ILSLQE   L AYNF+ SGEK+G                               +E L
Sbjct: 1090 SDILSLQE--RLAAYNFESSGEKSGENVVEVVKPKGRAGSKNTQKKAGDESDILSLQERL 1147

Query: 724  GAYNLIPSGEKTGALETEEPPLPAGKKQNNRKGGAKK--TTVVLDTDSENEVGXXXXXXX 897
             AYN   SGEK+GA+E+EEP +PAGKKQ N++ GAKK  +T+VL++DS+NE         
Sbjct: 1148 AAYNFESSGEKSGAMESEEP-VPAGKKQINKRAGAKKMLSTIVLESDSDNE-----DDGN 1201

Query: 898  FELVQQPAPEALKKEGGR----------------NLGTKRTFGQMLLTDMLQPAEGTGTS 1029
            FE+ Q+ AP    K+GGR                N+GTK+T G+ LLT MLQPAE T T 
Sbjct: 1202 FEVQQKAAPT---KKGGRKPAAQNTKAPALPKKRNVGTKQTLGKKLLTGMLQPAESTAT- 1257

Query: 1030 SPEKKVRKMRESPFNKGKSPSEDLSWGSGS 1119
            SPEKK RKMRES         EDLS GS S
Sbjct: 1258 SPEKKARKMRESSI-------EDLSAGSAS 1280


>ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago truncatula]
 gb|AES69522.2| DNA topoisomerase IV subunit A [Medicago truncatula]
          Length = 1504

 Score =  218 bits (555), Expect = 9e-59
 Identities = 186/516 (36%), Positives = 236/516 (45%), Gaps = 99/516 (19%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYYEKR++ IL+NL+RLL +L N V+FIL  +  ++   +   T+LL+ELK++GFTP+
Sbjct: 1003 RLEYYEKRKKYILANLERLLLILDNKVRFILGVVSGEIIVSNRKRTELLIELKQKGFTPI 1062

Query: 178  PREGKSTS---TGANDDNLEALKD------------------DYEYLLYLPYKAFVIEEV 294
            P++GKS      GAND+N E  +D                  DYEYLL L      +E V
Sbjct: 1063 PKKGKSAEPQVAGANDENSEDQEDSERETASKSVNVEGATWGDYEYLLSLSIGTLTLESV 1122

Query: 295  QELLAEMNEKKKQFDVLKTTTAKSLWMKD-------LNELEKKLDEIYGXXXXXXXXXXX 453
            Q+LL E  +K+ +F++L  T +KSLW+KD       L+EL++   E              
Sbjct: 1123 QKLLDEKADKENEFEILMGTPSKSLWLKDLDELEKKLDELDRIEAEEGQKRSTMSNKKNR 1182

Query: 454  XXXXXXXXXXXXXXXXXXXNV--------------------VKVTKPKGIAGCMKIQKDS 573
                               NV                    V+V KPKG AG  K    +
Sbjct: 1183 LATKPAKKPPQPRKNTKKANVEPENDNSSMEIENAVEVVKPVEVAKPKGRAGSKK-NAQT 1241

Query: 574  AATDAILSLQEFQGLDAYNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGE 753
             A D ILSLQE                                      L  YNL  SGE
Sbjct: 1242 EADDEILSLQE-------------------------------------RLAVYNLESSGE 1264

Query: 754  KTGALETEEPPLPAGKKQNNRKGGAKK--TTVVLDTDSENEVGXXXXXXX-FELVQQPAP 924
            K+ A+E E P  PAGKKQ N++GGAKK   T+VL++DS+NEV         FE+ +  AP
Sbjct: 1265 KSQAMEEEPPVPPAGKKQINKRGGAKKKSNTIVLESDSDNEVNDVEDDDDDFEVQENAAP 1324

Query: 925  E----------------------------ALKKEGGR-------------------NLGT 963
                                         A  K+GGR                   N+G+
Sbjct: 1325 AKKGGRKPAAQNAKKAAAEDDEFEVQEKAAPAKKGGRKAVQNAKPPAKAAAAPKKRNVGS 1384

Query: 964  KRTFGQMLLTDMLQPAEGTGTSSPEKKVRKMRESPFNKGKSPSEDLSWGSGSAKSGSILG 1143
            K T GQ LLTDMLQPAE  GT SPEKKVRKMRESPFNK               KSGSILG
Sbjct: 1385 KSTLGQKLLTDMLQPAESIGT-SPEKKVRKMRESPFNK---------------KSGSILG 1428

Query: 1144 RAANRDKSQSEDLSLGSGSATRFPLNFEEDVAEIAP 1251
            + AN D S   DLS GS S +      E++V EIAP
Sbjct: 1429 KTANMDISPIADLSEGSTSNSP---TAEDEVVEIAP 1461


>ref|XP_017425524.1| PREDICTED: DNA topoisomerase 2 [Vigna angularis]
 gb|KOM42453.1| hypothetical protein LR48_Vigan05g005700 [Vigna angularis]
 dbj|BAT93356.1| hypothetical protein VIGAN_07230300 [Vigna angularis var. angularis]
          Length = 1480

 Score =  216 bits (550), Expect = 4e-58
 Identities = 173/474 (36%), Positives = 236/474 (49%), Gaps = 56/474 (11%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RL+YYE+R+ +IL+NL+RLL +L N V+FIL  +  ++   +    DL++EL+++GFTPM
Sbjct: 1008 RLDYYERRKNHILNNLERLLLVLDNKVRFILGVVSGEIIVSNRKNADLVIELQQKGFTPM 1067

Query: 178  PREGKSTS---TGANDD--------------NLEALK-DDYEYLLYLPYKAFVIEEVQEL 303
            PR+GKS      GAND+              ++EA K  DYEYLL +  +    E VQ+L
Sbjct: 1068 PRKGKSAEPVVAGANDEEEQEDDNSTEQETGSVEAAKRGDYEYLLAMSIRTLTFESVQKL 1127

Query: 304  LAEMNEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEIYGXXXXXXXXXXXXXXXXXXXXX 483
            LAE  EK+K+F+VLK T +KS+W+KDL+ELEKKLDEI                       
Sbjct: 1128 LAEKAEKEKEFEVLKATPSKSMWLKDLDELEKKLDEI-------------------DSKD 1168

Query: 484  XXXXXXXXXNVVKVTKPKGIAGCMKIQK------------DSAATDAILSLQEFQGLDAY 627
                        +    +G++   K  K             +   D   S  E + + A 
Sbjct: 1169 EEEERKRSSQATRKASNRGVSAATKAAKKPPQPRKYTKKAKNVEPDNDDSSMEVENV-AE 1227

Query: 628  NFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGALETEEPPLPAGKKQ 807
              KP G   G+                 +E L AYN  PS + +  +ETEE P P GK  
Sbjct: 1228 VTKPKGRAAGS---ENTQTVGDDEIASLQERLAAYNFAPSSDLSTGMETEERPAPTGKTA 1284

Query: 808  NNRKGGA--KKTTVVL-DTDSENEVGXXXXXXXFELVQQPAPEALKKEGG---------- 948
              R+G A  K +TVVL DTDS+N+         F + Q     + KK+GG          
Sbjct: 1285 PKRRGPAAKKSSTVVLDDTDSDND-NDDSNDNDFGITQPSTAGSGKKKGGRKPAAQNAKK 1343

Query: 949  -------RNLGTKRT--FGQMLLTDMLQPAEGTGTSSPEKKVRKMRESPFNKGKSPSEDL 1101
                   RN+G K++   GQ L+TDM+   E TG  SPEKKVRKMR SPFNK        
Sbjct: 1344 APAATRKRNVGGKQSQVLGQKLITDMM---EKTGI-SPEKKVRKMRASPFNK-------- 1391

Query: 1102 SWGSGSAKSGSILGR---AANRDKSQSEDLSLGSGSATRFPLNFEEDVAEIAPP 1254
                   KS S+LGR   AA++++S SEDLS GS  +   P   EE+V E+APP
Sbjct: 1392 -------KSSSVLGRVATAADKEESVSEDLSGGSAGSNPSPSIAEEEVVEVAPP 1438


>gb|KHN45085.1| DNA topoisomerase 2, partial [Glycine soja]
          Length = 1379

 Score =  213 bits (542), Expect = 5e-57
 Identities = 173/467 (37%), Positives = 247/467 (52%), Gaps = 49/467 (10%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKMK-RHISETDLLMELKRQGFTPM 177
            RLEYYE+R+++IL+NL++LL +L N V+FIL  +  ++   +  + +LL+EL+++GFTPM
Sbjct: 907  RLEYYERRKKHILNNLEQLLLILDNKVRFILGVVNGEIVVSNRKKAELLIELQQKGFTPM 966

Query: 178  PREGKSTS---TGANDD---------------NLEALK-DDYEYLLYLPYKAFVIEEVQE 300
            PR+GKS      GAND+               ++E +K  DYEYLL +      +E VQ+
Sbjct: 967  PRKGKSAEPQVAGANDEEEQEDDNNTREQETGSVEGVKRGDYEYLLSMSIGTLTLEAVQK 1026

Query: 301  LLAEMNEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEIYGXXXXXXXXXXXXXXXXXXXX 480
            LLAE  EK+K+F++LK T +KSLW+KDL+ELE KLD+I                      
Sbjct: 1027 LLAEKAEKEKEFEILKATPSKSLWLKDLDELENKLDDIDSIEAEEERKRSSQANRKTSNR 1086

Query: 481  XXXXXXXXXXNVVKVTKPKGIAGCMKIQKDSAATDAILSLQEFQGLDAYNFKPSGEKTGA 660
                         K TK    A  ++ + D+++ + I ++ E         KP G + G+
Sbjct: 1087 GVTKAAKKPPQPRKYTKK---AKNVEPENDNSSME-IENVAEVA-------KPKG-RAGS 1134

Query: 661  XXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGALETEEPPLPAGKKQN-NRKGGAKKT 837
                             +E L AYN   S + +  +ETEEPP PA KK+   R+G AKK 
Sbjct: 1135 KNTQNTQPVAEDEIVSLQERLAAYNFGASSDISEGMETEEPPAPAEKKEGPKRRGAAKKK 1194

Query: 838  T---VVLDTDSENE---VGXXXXXXXFELVQQPAPEALKKEGG----------------- 948
            +   V+ D+DS+NE   V        FE++Q  AP   +K+GG                 
Sbjct: 1195 SSAIVLDDSDSDNEVHNVDDDDDDEDFEIMQPVAPG--RKKGGRKPAAQNAKKAPAAAAR 1252

Query: 949  -RNLGTKRT--FGQMLLTDMLQPAEGTGTSSPEKKVRKMRESPFNKGKSPSEDLSWGSGS 1119
             RN+G K++   GQ L+TDML   E TG  SPEKKVRKMR SPFNK              
Sbjct: 1253 KRNVGGKQSQFLGQKLITDML---ESTGI-SPEKKVRKMRASPFNK-------------- 1294

Query: 1120 AKSGSILGR--AANRDKSQSEDLSLGSGSATRFPLNFEEDVAEIAPP 1254
             KS S+LGR  AA++D+S S+DLS GS +++  P +  E+V EIAPP
Sbjct: 1295 -KSSSVLGRVAAADKDESVSKDLSGGSDASSSSP-SITEEVVEIAPP 1339


>ref|XP_003545986.1| PREDICTED: DNA topoisomerase 2-like [Glycine max]
 gb|KRH13973.1| hypothetical protein GLYMA_15G276000 [Glycine max]
          Length = 1484

 Score =  210 bits (534), Expect = 6e-56
 Identities = 172/467 (36%), Positives = 246/467 (52%), Gaps = 49/467 (10%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKMK-RHISETDLLMELKRQGFTPM 177
            RLEYYE+R+++IL+NL++LL +L N V+FIL  +  ++   +  + +LL+EL+++GFTPM
Sbjct: 1012 RLEYYERRKKHILNNLEQLLLILDNKVRFILGVVNGEIVVSNRKKAELLIELQQKGFTPM 1071

Query: 178  PREGKSTS---TGANDD---------------NLEALK-DDYEYLLYLPYKAFVIEEVQE 300
            PR+GKS      GAND+               ++E +K  DYEYLL +      +E VQ+
Sbjct: 1072 PRKGKSAEPQVAGANDEEEQEDDNNTREQETGSVEGVKRGDYEYLLSMSIGTLTLEAVQK 1131

Query: 301  LLAEMNEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEIYGXXXXXXXXXXXXXXXXXXXX 480
            LLAE  EK+K+F++LK T +KSLW+KDL+ELE KLD+I                      
Sbjct: 1132 LLAEKAEKEKEFEILKATPSKSLWLKDLDELENKLDDIDSIEAEEERKRSSQANRKTSNR 1191

Query: 481  XXXXXXXXXXNVVKVTKPKGIAGCMKIQKDSAATDAILSLQEFQGLDAYNFKPSGEKTGA 660
                         K TK    A  ++ + D+++ + I ++ E         KP G + G+
Sbjct: 1192 GVTKAAKKPPQPRKYTKK---AKNVEPENDNSSME-IENVAEVA-------KPKG-RAGS 1239

Query: 661  XXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGALETEEPPLPAGKKQN-NRKGGAKKT 837
                             +E L AYN   S + +  +ETEEP  PA KK+   R+G AKK 
Sbjct: 1240 KNTQNTQPVAEDEIVSLQERLAAYNFGASSDISEGMETEEPSAPAEKKEGPKRRGAAKKK 1299

Query: 838  T---VVLDTDSENE---VGXXXXXXXFELVQQPAPEALKKEGG----------------- 948
            +   V+ D+DS+NE   V        FE++Q  AP   +K+GG                 
Sbjct: 1300 SSAIVLDDSDSDNEVHNVDDDDDDEDFEIMQPVAPG--RKKGGRKPAAQNAKKAPAAAAR 1357

Query: 949  -RNLGTKRT--FGQMLLTDMLQPAEGTGTSSPEKKVRKMRESPFNKGKSPSEDLSWGSGS 1119
             RN+G K++   GQ L+TDML   E TG  SPEKKVRKMR SPFNK              
Sbjct: 1358 KRNVGGKQSQFLGQKLITDML---ESTGI-SPEKKVRKMRASPFNK-------------- 1399

Query: 1120 AKSGSILGR--AANRDKSQSEDLSLGSGSATRFPLNFEEDVAEIAPP 1254
             KS S+LGR  AA++D+S S+DLS GS +++  P +  E+V EIAPP
Sbjct: 1400 -KSSSVLGRVAAADKDESVSKDLSGGSDASSSSP-SITEEVVEIAPP 1444


>ref|XP_016170361.1| DNA topoisomerase 2 isoform X2 [Arachis ipaensis]
          Length = 1486

 Score =  210 bits (534), Expect = 6e-56
 Identities = 179/483 (37%), Positives = 235/483 (48%), Gaps = 66/483 (13%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYY +R+++IL+NL+RLL +L N V+FIL  +  ++   +  + DLLMEL+++GFTPM
Sbjct: 1015 RLEYYGRRKQHILNNLERLLLILDNKVRFILGVVNGEIIVSNRKKADLLMELQQKGFTPM 1074

Query: 178  PREGKSTS---TGANDDNLE----------------ALKDDYEYLLYLPYKAFVIEEVQE 300
            PR+GKS      GANDD  E                A + DYEYLL LP     IE VQ+
Sbjct: 1075 PRKGKSAEPQVAGANDDISEEQDDDTTREETATVEGAKRGDYEYLLSLPIGTLTIESVQK 1134

Query: 301  LLAEMNEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEIYG------XXXXXXXXXXXXXX 462
            LLAE +EK+K+F++LK T   S+WMKDL+ELEKKLDE+                      
Sbjct: 1135 LLAEKDEKEKEFEILKATPPTSMWMKDLDELEKKLDEMDSKAAEEQQKRSSQGNRKTSNR 1194

Query: 463  XXXXXXXXXXXXXXXXNVVKVTK--PKGIAGCMKIQ-----------------KDSAATD 585
                            N  K TK  P+     M+I+                 K     D
Sbjct: 1195 GISAAAAAKKPPQPRKNTKKATKAEPENDNSSMEIENHVEVAKPKGRPKKAPAKKVVDED 1254

Query: 586  AILSLQEFQGLDAYNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGA 765
             ILSLQE   L AYNF  S +++ A                                  A
Sbjct: 1255 EILSLQE--RLAAYNFGASSDQSSA----------------------------------A 1278

Query: 766  LETEEPP-LPAGKKQNNRKGGAKK---TTVVLD-TDSENEVGXXXXXXXFELVQQPAPEA 930
            +E E PP  PA +K   + G AKK   +T++LD +DS+N          FE++ Q A EA
Sbjct: 1279 MEAEAPPEAPAARK--GKTGRAKKKSSSTILLDSSDSDNVEDEDDGDDDFEILDQAASEA 1336

Query: 931  LKKEGG----------------RNLGTKRTFGQMLLTDMLQPAEGTGTSSPEKKVRKMRE 1062
             KK+GG                RNLG+KR+  Q L T+ML+PAE    +SPEKKVR+MR 
Sbjct: 1337 GKKKGGRKPAAHKAAKAPAATKRNLGSKRS--QKLSTEMLKPAE--EETSPEKKVRRMRP 1392

Query: 1063 SPFNKGKSPSEDLSWGSGSAKSGSILGRAANRDKSQSEDLSLGSGSATRFPLNFEEDVAE 1242
            SPFNK               KSGS+LGR A +D++ SE    GS SA   P   EE+V E
Sbjct: 1393 SPFNK---------------KSGSLLGRLAEKDQNASE----GSASANS-PSTTEEEVVE 1432

Query: 1243 IAP 1251
            +AP
Sbjct: 1433 VAP 1435


>ref|XP_016170360.1| DNA topoisomerase 2 isoform X1 [Arachis ipaensis]
          Length = 1517

 Score =  210 bits (534), Expect = 6e-56
 Identities = 179/483 (37%), Positives = 235/483 (48%), Gaps = 66/483 (13%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYY +R+++IL+NL+RLL +L N V+FIL  +  ++   +  + DLLMEL+++GFTPM
Sbjct: 1046 RLEYYGRRKQHILNNLERLLLILDNKVRFILGVVNGEIIVSNRKKADLLMELQQKGFTPM 1105

Query: 178  PREGKSTS---TGANDDNLE----------------ALKDDYEYLLYLPYKAFVIEEVQE 300
            PR+GKS      GANDD  E                A + DYEYLL LP     IE VQ+
Sbjct: 1106 PRKGKSAEPQVAGANDDISEEQDDDTTREETATVEGAKRGDYEYLLSLPIGTLTIESVQK 1165

Query: 301  LLAEMNEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEIYG------XXXXXXXXXXXXXX 462
            LLAE +EK+K+F++LK T   S+WMKDL+ELEKKLDE+                      
Sbjct: 1166 LLAEKDEKEKEFEILKATPPTSMWMKDLDELEKKLDEMDSKAAEEQQKRSSQGNRKTSNR 1225

Query: 463  XXXXXXXXXXXXXXXXNVVKVTK--PKGIAGCMKIQ-----------------KDSAATD 585
                            N  K TK  P+     M+I+                 K     D
Sbjct: 1226 GISAAAAAKKPPQPRKNTKKATKAEPENDNSSMEIENHVEVAKPKGRPKKAPAKKVVDED 1285

Query: 586  AILSLQEFQGLDAYNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGA 765
             ILSLQE   L AYNF  S +++ A                                  A
Sbjct: 1286 EILSLQE--RLAAYNFGASSDQSSA----------------------------------A 1309

Query: 766  LETEEPP-LPAGKKQNNRKGGAKK---TTVVLD-TDSENEVGXXXXXXXFELVQQPAPEA 930
            +E E PP  PA +K   + G AKK   +T++LD +DS+N          FE++ Q A EA
Sbjct: 1310 MEAEAPPEAPAARK--GKTGRAKKKSSSTILLDSSDSDNVEDEDDGDDDFEILDQAASEA 1367

Query: 931  LKKEGG----------------RNLGTKRTFGQMLLTDMLQPAEGTGTSSPEKKVRKMRE 1062
             KK+GG                RNLG+KR+  Q L T+ML+PAE    +SPEKKVR+MR 
Sbjct: 1368 GKKKGGRKPAAHKAAKAPAATKRNLGSKRS--QKLSTEMLKPAE--EETSPEKKVRRMRP 1423

Query: 1063 SPFNKGKSPSEDLSWGSGSAKSGSILGRAANRDKSQSEDLSLGSGSATRFPLNFEEDVAE 1242
            SPFNK               KSGS+LGR A +D++ SE    GS SA   P   EE+V E
Sbjct: 1424 SPFNK---------------KSGSLLGRLAEKDQNASE----GSASANS-PSTTEEEVVE 1463

Query: 1243 IAP 1251
            +AP
Sbjct: 1464 VAP 1466


>ref|XP_014501355.1| DNA topoisomerase 2 [Vigna radiata var. radiata]
          Length = 1492

 Score =  208 bits (529), Expect = 3e-55
 Identities = 173/485 (35%), Positives = 238/485 (49%), Gaps = 67/485 (13%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RL+YYE+R+ +IL+NL+RLL +L N V+FIL  +  ++   +    DL++EL+++GFTPM
Sbjct: 1008 RLDYYERRKNHILNNLERLLLVLDNKVRFILGVVSGEIIVNNRKNADLVIELQQKGFTPM 1067

Query: 178  PREGKSTS---TGANDD--------------NLEALK-DDYEYLLYLPYKAFVIEEVQEL 303
            PR+GKS      GAND+              ++EA K  DYEYLL +  +    E VQ+L
Sbjct: 1068 PRKGKSAEPVVAGANDEEEQEDDNSTEQETGSVEAAKRGDYEYLLAMSIRTLTFESVQKL 1127

Query: 304  LAEMNEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEIYGXXXXXXXXXXXXXXXXXXXXX 483
            LAE  EK+K+F++LK T +KS+W+KDL+ELEKKLDEI                       
Sbjct: 1128 LAEKAEKEKEFEILKATPSKSMWLKDLDELEKKLDEI-------------------DSKE 1168

Query: 484  XXXXXXXXXNVVKVTKPKGIAGCMKIQK-------------DSAATDAILSLQEFQGLDA 624
                        +    +G++   K  K              +   D   S  E + + A
Sbjct: 1169 AEEELKRSSQATRKASNRGVSAATKAAKKPPPQPRKYTKKAKNVEPDNDNSSMEVENV-A 1227

Query: 625  YNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGALETEEPPLPAGKK 804
               KP G   G+                 +E L AYN  PS +++  +ETEE P PAGK 
Sbjct: 1228 EVTKPKGRAAGS---ENTQTVGDDEIASLQERLAAYNFAPSSDQSTGMETEERPAPAGKT 1284

Query: 805  QNNRKGGA--KKTTVVLD-TDSENEVGXXXXXXXFELVQQPAPEALKKEGG--------- 948
               R+G A  K T +VLD TDS+N+         FE + QP+  + KK+GG         
Sbjct: 1285 APKRRGPAAKKSTAIVLDETDSDND-NDDTNDNDFE-ITQPSAGSGKKKGGRKPAAQNAK 1342

Query: 949  -----------------RNLGTKRT--FGQMLLTDMLQPAEGTGTSSPEKKVRKMRESPF 1071
                             RN+G K++   GQ L+TDM+   E TG  SPEKKVRKMR SPF
Sbjct: 1343 KAPAAQNAKKAPAATRKRNVGGKQSQVLGQKLITDMM---EKTGI-SPEKKVRKMRASPF 1398

Query: 1072 NKGKSPSEDLSWGSGSAKSGSILGR---AANRDKSQSEDLS-LGSGSATRFPLNFEEDVA 1239
            NK               KS S+LGR   AAN ++S SEDLS  GS  +   P   E++V 
Sbjct: 1399 NK---------------KSSSVLGRVAAAANIEESVSEDLSGGGSAGSNPSPGTAEDEVV 1443

Query: 1240 EIAPP 1254
            E+A P
Sbjct: 1444 EVAAP 1448


>gb|KRH32967.1| hypothetical protein GLYMA_10G090000 [Glycine max]
          Length = 1459

 Score =  203 bits (517), Expect = 1e-53
 Identities = 179/490 (36%), Positives = 235/490 (47%), Gaps = 72/490 (14%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYYE+R+ +IL+NL+RLL +L N  +FIL  +  ++   +  + DLL+EL+ +GFTPM
Sbjct: 989  RLEYYERRKNHILNNLERLLLILDNKARFILGVVNGEIIVSNRKKADLLIELQHKGFTPM 1048

Query: 178  PREGKSTS---TGANDD------------NLEALKD-DYEYLLYLPYKAFVIEEVQELLA 309
            PR+GKS      GAN+D            ++E  K  DYEYLL +      +E VQ+LLA
Sbjct: 1049 PRKGKSAEPQVAGANEDQEDDNTREQETESVEVAKGGDYEYLLSMSIGTLTLEAVQKLLA 1108

Query: 310  EMNEKKKQFDVLKTTTAKSLWMKDLN----------------------ELEKKLDEIYGX 423
            E  EK+K+F +LK T +KSLW+KDL+                      +  +K  E +  
Sbjct: 1109 EKAEKEKEFKILKETPSKSLWLKDLDELEKKLDDIDSIEAEEERKRSSQANRKTKEAFTK 1168

Query: 424  XXXXXXXXXXXXXXXXXXXXXXXXXXXXX-NVVKVTKPKGIAGCMKIQKDS---AATDAI 591
                                          NV +V KPKG AG    QK++   A  D I
Sbjct: 1169 AAKKPPQPRKYTKKAKNVEPENDNSSMEVENVAEVVKPKGRAGSNNTQKNTLPVAEDDEI 1228

Query: 592  LSLQEFQGLDAYNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGALE 771
            LSLQE                                      L AYN   S + +  +E
Sbjct: 1229 LSLQE-------------------------------------RLAAYNFGASSDISAGME 1251

Query: 772  TEEPPLPAGKKQ-NNRKGGAKKTT---VVLDTDSENEV-GXXXXXXXFELVQQPAPEALK 936
            TEEPP PA KK+   R+G AKK +   V+ D+DS+NE+         FE++Q  A EA K
Sbjct: 1252 TEEPPAPAEKKEAPKRRGAAKKMSSAIVLDDSDSDNEIHDVEDDDDNFEIMQPVALEAGK 1311

Query: 937  KEGG-------------------RNLGTKRT--FGQMLLTDMLQPAEGTGTSSPEKKVRK 1053
            K+GG                   RN+G K++   GQ L+TDML   E TG  SPEKKVRK
Sbjct: 1312 KKGGRKPAAQNAKKAPAAASTKKRNVGGKQSQLLGQKLITDML---ESTGI-SPEKKVRK 1367

Query: 1054 MRESPFNKGKSPSEDLSWGSGSAKSGSILGR--AANRDKSQSEDLSLGS-GSATRFPLNF 1224
            MR SPFNK               KS S+LGR  AA++D S SEDLS  S GS+ R     
Sbjct: 1368 MRASPFNK---------------KSSSVLGRVAAADKDDSVSEDLSGDSAGSSPR----I 1408

Query: 1225 EEDVAEIAPP 1254
             E+V EIAPP
Sbjct: 1409 TEEVVEIAPP 1418


>ref|XP_014618555.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisomerase 2-like [Glycine
            max]
          Length = 1481

 Score =  203 bits (517), Expect = 1e-53
 Identities = 179/490 (36%), Positives = 235/490 (47%), Gaps = 72/490 (14%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYYE+R+ +IL+NL+RLL +L N  +FIL  +  ++   +  + DLL+EL+ +GFTPM
Sbjct: 1011 RLEYYERRKNHILNNLERLLLILDNKARFILGVVNGEIIVSNRKKADLLIELQHKGFTPM 1070

Query: 178  PREGKSTS---TGANDD------------NLEALKD-DYEYLLYLPYKAFVIEEVQELLA 309
            PR+GKS      GAN+D            ++E  K  DYEYLL +      +E VQ+LLA
Sbjct: 1071 PRKGKSAEPQVAGANEDQEDDNTREQETESVEVAKGGDYEYLLSMSIGTLTLEAVQKLLA 1130

Query: 310  EMNEKKKQFDVLKTTTAKSLWMKDLN----------------------ELEKKLDEIYGX 423
            E  EK+K+F +LK T +KSLW+KDL+                      +  +K  E +  
Sbjct: 1131 EKAEKEKEFKILKETPSKSLWLKDLDELEKKLDDIDSIEAEEERKRSSQANRKTKEAFTK 1190

Query: 424  XXXXXXXXXXXXXXXXXXXXXXXXXXXXX-NVVKVTKPKGIAGCMKIQKDS---AATDAI 591
                                          NV +V KPKG AG    QK++   A  D I
Sbjct: 1191 AAKKPPQPRKYTKKAKNVEPENDNSSMEVENVAEVVKPKGRAGSNNTQKNTLPVAEDDEI 1250

Query: 592  LSLQEFQGLDAYNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGALE 771
            LSLQE                                      L AYN   S + +  +E
Sbjct: 1251 LSLQE-------------------------------------RLAAYNFGASSDISAGME 1273

Query: 772  TEEPPLPAGKKQ-NNRKGGAKKTT---VVLDTDSENEV-GXXXXXXXFELVQQPAPEALK 936
            TEEPP PA KK+   R+G AKK +   V+ D+DS+NE+         FE++Q  A EA K
Sbjct: 1274 TEEPPAPAEKKEAPKRRGAAKKMSSAIVLDDSDSDNEIHDVEDDDDNFEIMQPVALEAGK 1333

Query: 937  KEGG-------------------RNLGTKRT--FGQMLLTDMLQPAEGTGTSSPEKKVRK 1053
            K+GG                   RN+G K++   GQ L+TDML   E TG  SPEKKVRK
Sbjct: 1334 KKGGRKPAAQNAKKAPAAASTKKRNVGGKQSQLLGQKLITDML---ESTGI-SPEKKVRK 1389

Query: 1054 MRESPFNKGKSPSEDLSWGSGSAKSGSILGR--AANRDKSQSEDLSLGS-GSATRFPLNF 1224
            MR SPFNK               KS S+LGR  AA++D S SEDLS  S GS+ R     
Sbjct: 1390 MRASPFNK---------------KSSSVLGRVAAADKDDSVSEDLSGDSAGSSPR----I 1430

Query: 1225 EEDVAEIAPP 1254
             E+V EIAPP
Sbjct: 1431 TEEVVEIAPP 1440


>ref|XP_015936924.1| DNA topoisomerase 2 isoform X2 [Arachis duranensis]
          Length = 1490

 Score =  201 bits (512), Expect = 5e-53
 Identities = 173/483 (35%), Positives = 230/483 (47%), Gaps = 66/483 (13%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYY KR+++IL+NL+RLL +L N V+FIL  +  ++   +  + DLLMEL+++GFTPM
Sbjct: 1015 RLEYYGKRKQHILNNLERLLLILDNKVRFILGVVNGEIIVSNRKKADLLMELQQKGFTPM 1074

Query: 178  PREGKSTS---TGANDDNLE----------------ALKDDYEYLLYLPYKAFVIEEVQE 300
            PR+GKS      GANDD  E                A + DYEYLL LP  +  IE VQ+
Sbjct: 1075 PRKGKSAEPQVAGANDDISEEQDDDTTREETATVEGAKRGDYEYLLSLPIGSLTIESVQK 1134

Query: 301  LLAEMNEKKKQFDVLKTTTAKSLWMKDL-------NELEKKLDE---------------- 411
            LLAE +EK+K+F++LK T   S+WMKDL       +E++ K  E                
Sbjct: 1135 LLAEKDEKEKEFEILKATPPTSMWMKDLDELEKKLDEMDSKAAEEQQKRSSQGNRKTSNR 1194

Query: 412  ---IYGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNVVKVTKPKGIAGCMKIQKDSAAT 582
                                                N V+V KPKG       +K     
Sbjct: 1195 GISAAAAAKKPPQPRKNTKKAKNVEPENDNSSMEIENHVEVAKPKGRPKKAPAKK-VVDE 1253

Query: 583  DAILSLQEFQGLDAYNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTG 762
            D ILSLQE   L AYNF  S +++ A                                  
Sbjct: 1254 DEILSLQE--RLAAYNFGASSDQSSA---------------------------------- 1277

Query: 763  ALETEEPPLPAGKKQNNRKGGAKK---TTVVLDT-DSENEVGXXXXXXXFELVQQPAPEA 930
            A+E E PP     ++    G AKK   +T++LD+ DS+N          FE++ Q A EA
Sbjct: 1278 AMEAEVPPEAPAARKGKTTGRAKKKSSSTILLDSSDSDNVEDEDDEDDDFEILDQAASEA 1337

Query: 931  LKKEGG----------------RNLGTKRTFGQMLLTDMLQPAEGTGTSSPEKKVRKMRE 1062
             KK+GG                RNLG+KR+  Q L T+ML+PAE    +SPEKKVR+MR 
Sbjct: 1338 GKKKGGRKPAAQKAAKAPAATKRNLGSKRS--QKLSTEMLKPAE--EETSPEKKVRRMRP 1393

Query: 1063 SPFNKGKSPSEDLSWGSGSAKSGSILGRAANRDKSQSEDLSLGSGSATRFPLNFEEDVAE 1242
            SPFNK               KSGS+LGR A +D++ SEDLS GS S+   P    E+V E
Sbjct: 1394 SPFNK---------------KSGSLLGRLAEKDQNASEDLS-GSASSNS-PSTTGEEVVE 1436

Query: 1243 IAP 1251
            +AP
Sbjct: 1437 VAP 1439


>ref|XP_015936922.1| DNA topoisomerase 2 isoform X1 [Arachis duranensis]
          Length = 1521

 Score =  201 bits (512), Expect = 5e-53
 Identities = 173/483 (35%), Positives = 230/483 (47%), Gaps = 66/483 (13%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYY KR+++IL+NL+RLL +L N V+FIL  +  ++   +  + DLLMEL+++GFTPM
Sbjct: 1046 RLEYYGKRKQHILNNLERLLLILDNKVRFILGVVNGEIIVSNRKKADLLMELQQKGFTPM 1105

Query: 178  PREGKSTS---TGANDDNLE----------------ALKDDYEYLLYLPYKAFVIEEVQE 300
            PR+GKS      GANDD  E                A + DYEYLL LP  +  IE VQ+
Sbjct: 1106 PRKGKSAEPQVAGANDDISEEQDDDTTREETATVEGAKRGDYEYLLSLPIGSLTIESVQK 1165

Query: 301  LLAEMNEKKKQFDVLKTTTAKSLWMKDL-------NELEKKLDE---------------- 411
            LLAE +EK+K+F++LK T   S+WMKDL       +E++ K  E                
Sbjct: 1166 LLAEKDEKEKEFEILKATPPTSMWMKDLDELEKKLDEMDSKAAEEQQKRSSQGNRKTSNR 1225

Query: 412  ---IYGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNVVKVTKPKGIAGCMKIQKDSAAT 582
                                                N V+V KPKG       +K     
Sbjct: 1226 GISAAAAAKKPPQPRKNTKKAKNVEPENDNSSMEIENHVEVAKPKGRPKKAPAKK-VVDE 1284

Query: 583  DAILSLQEFQGLDAYNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTG 762
            D ILSLQE   L AYNF  S +++ A                                  
Sbjct: 1285 DEILSLQE--RLAAYNFGASSDQSSA---------------------------------- 1308

Query: 763  ALETEEPPLPAGKKQNNRKGGAKK---TTVVLDT-DSENEVGXXXXXXXFELVQQPAPEA 930
            A+E E PP     ++    G AKK   +T++LD+ DS+N          FE++ Q A EA
Sbjct: 1309 AMEAEVPPEAPAARKGKTTGRAKKKSSSTILLDSSDSDNVEDEDDEDDDFEILDQAASEA 1368

Query: 931  LKKEGG----------------RNLGTKRTFGQMLLTDMLQPAEGTGTSSPEKKVRKMRE 1062
             KK+GG                RNLG+KR+  Q L T+ML+PAE    +SPEKKVR+MR 
Sbjct: 1369 GKKKGGRKPAAQKAAKAPAATKRNLGSKRS--QKLSTEMLKPAE--EETSPEKKVRRMRP 1424

Query: 1063 SPFNKGKSPSEDLSWGSGSAKSGSILGRAANRDKSQSEDLSLGSGSATRFPLNFEEDVAE 1242
            SPFNK               KSGS+LGR A +D++ SEDLS GS S+   P    E+V E
Sbjct: 1425 SPFNK---------------KSGSLLGRLAEKDQNASEDLS-GSASSNS-PSTTGEEVVE 1467

Query: 1243 IAP 1251
            +AP
Sbjct: 1468 VAP 1470


>gb|PNY06816.1| DNA topoisomerase 2-like protein [Trifolium pratense]
          Length = 1254

 Score =  195 bits (496), Expect = 6e-51
 Identities = 161/436 (36%), Positives = 211/436 (48%), Gaps = 63/436 (14%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            R+EYYE+R++ IL+NL+RLL +L N V+FIL  +  ++   +  + +LL+ELK++GFTPM
Sbjct: 818  RMEYYERRKKYILANLERLLLILDNKVRFILGVVNGEIIVSNRKKAELLIELKQKGFTPM 877

Query: 178  PREGKSTS---TGANDDNLEALKD-----------------DYEYLLYLPYKAFVIEEVQ 297
             R+GKS      GANDDN E  +D                 DYEYLL L      +E VQ
Sbjct: 878  -RKGKSAEPQVAGANDDNSEGQEDEQETVSRPVSVEGATWGDYEYLLSLSIGTLTLESVQ 936

Query: 298  ELLAEMNEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEIYGXXXXXXXXXXXXXXXXXXX 477
            +LL E  EK+K++++L  T  KSLW+KDL++ EKKLDE+                     
Sbjct: 937  KLLDEKAEKEKEYEILMGTPTKSLWLKDLDDFEKKLDELDSKEAEDDRKRSSQASKKTAN 996

Query: 478  XXXXXXXXXXXNVVKVTK------------------------PKGIAGCMKIQKDSAATD 585
                          K TK                        PKG  G    QK +    
Sbjct: 997  GFAAKPAKKPPQPRKNTKKNKDVEPENDNSSMEVENAVEVVKPKGRVGSKNTQKKAGDES 1056

Query: 586  AILSLQEFQGLDAYNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGA 765
             I SLQE   L AYNF+ SGE                                   K+GA
Sbjct: 1057 DIPSLQE--RLAAYNFESSGE-----------------------------------KSGA 1079

Query: 766  LETEEPPLPAGKKQNNRKGGAKK--TTVVLDTDSENEVGXXXXXXXFELVQQPAPEALKK 939
            +E++E P+PAGKKQ N +G AKK  +T+VL +DS+NE         FE+ Q+ AP    K
Sbjct: 1080 MESDE-PVPAGKKQINERGDAKKMSSTIVLGSDSDNE-----DDDNFEVQQKAAP---AK 1130

Query: 940  EGG----------------RNLGTKRTFGQMLLTDMLQPAEGTGTSSPEKKVRKMRESPF 1071
            +GG                RN+GTK+T G+ LLT MLQPAE T T SP+KKVRKM     
Sbjct: 1131 KGGRKPAAQNAKAPALPKKRNVGTKQTLGKKLLTGMLQPAESTVT-SPKKKVRKM----- 1184

Query: 1072 NKGKSPSEDLSWGSGS 1119
              G+S  EDLS GS S
Sbjct: 1185 --GESSIEDLSAGSAS 1198


>ref|XP_007148908.1| hypothetical protein PHAVU_005G024200g [Phaseolus vulgaris]
 gb|ESW20902.1| hypothetical protein PHAVU_005G024200g [Phaseolus vulgaris]
          Length = 1474

 Score =  195 bits (496), Expect = 6e-51
 Identities = 165/484 (34%), Positives = 227/484 (46%), Gaps = 66/484 (13%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RL+YYE+R+  IL++L+RLL +L N V+FIL  +  ++   +    DL++EL+++GFTPM
Sbjct: 1009 RLDYYERRKNFILNDLERLLLVLDNKVRFILGVVSGEIIVSNRKNADLVLELQQKGFTPM 1068

Query: 178  PREGKSTS---TGANDDNLE----------------ALKDDYEYLLYLPYKAFVIEEVQE 300
            P++GKS      GAND+  +                A + DYEYLL +  +    E VQ+
Sbjct: 1069 PKKGKSAEPVVAGANDEEEQEDDNNSTEQETGSVESAKRGDYEYLLSMSIRTLTFESVQK 1128

Query: 301  LLAEMNEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEIYG--------XXXXXXXXXXXX 456
            LL E  EK+K+F++LK T +KS+W+KDL+ELEKKLDEI                      
Sbjct: 1129 LLGEKAEKEKEFEILKATPSKSMWLKDLDELEKKLDEIDSKDAEEERKRSTQATKKASNR 1188

Query: 457  XXXXXXXXXXXXXXXXXXNVVKVTKPKGIAGCMKIQKDSAAT------------------ 582
                                 K  +P+  +  M+++  +  T                  
Sbjct: 1189 ALGATKAAKKPPQPRKNTKKAKNVEPENDSSSMEVENVAQVTTNPPQGRGAGSEYTQTVG 1248

Query: 583  -DAILSLQEFQGLDAYNFKPSGEKTGAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKT 759
             D I SLQE   L AYNF PS +K+                                  T
Sbjct: 1249 EDEIASLQE--RLAAYNFAPSSDKS----------------------------------T 1272

Query: 760  GALETEEPPLPAGKKQNNRKGGA--KKTTVVLDTDSENEVGXXXXXXXFELVQQPAPEAL 933
            G +E EEPP PAGK    R+G A  KK+T+VLD  + ++          + + QPA    
Sbjct: 1273 G-MENEEPPAPAGKTVPKRRGPAAKKKSTIVLDDSASDD---DVKDDEEDEIMQPATG-- 1326

Query: 934  KKEGG---------------RNLGTKRT--FGQMLLTDMLQPAEGTGTSSPEKKVRKMRE 1062
            KK+GG               RN+G K++   GQ L+TDM+   E TG  SPEKKVRKMR 
Sbjct: 1327 KKKGGRKPAAAKKAPAATRKRNVGGKQSQLLGQKLITDMM---EKTGI-SPEKKVRKMRA 1382

Query: 1063 SPFNKGKSPSEDLSWGSGSAKSGSILGRAANRDKSQSEDLSLGSGSATRFPLNFEEDVAE 1242
            SPFNK               KS S+LGR A  DK +SEDLS GS  +   P    E+V E
Sbjct: 1383 SPFNK---------------KSSSVLGRIAADDKDESEDLSGGSAGSNSSP-GTSEEVVE 1426

Query: 1243 IAPP 1254
            +A P
Sbjct: 1427 VAQP 1430


>dbj|GAU30192.1| hypothetical protein TSUD_311430 [Trifolium subterraneum]
          Length = 1252

 Score =  192 bits (488), Expect = 7e-50
 Identities = 154/409 (37%), Positives = 206/409 (50%), Gaps = 36/409 (8%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            R+EYYE+R++ IL+NL+RLL ++ N V+FIL  +  ++   +  +T+LL+ELK++GFTPM
Sbjct: 818  RMEYYERRKKYILANLERLLLIVDNKVRFILGVVNGEIIVSNRKKTELLIELKQKGFTPM 877

Query: 178  PREGKSTS---TGANDDNLEALKD------------------DYEYLLYLPYKAFVIEEV 294
             R+GKS      GANDDN E  +D                  DYEYLL LP     +E V
Sbjct: 878  -RKGKSAEPQVAGANDDNSEGQEDGEQETVSRPVNVEGATWGDYEYLLSLPIGTLTLENV 936

Query: 295  QELLAEMNEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEIYGXXXXXXXXXXXXXXXXXX 474
            Q+LL E  EK+K++++L  T  KSLW+KDL++ EKKLDE+                    
Sbjct: 937  QKLLDEKAEKEKEYEILMGTPTKSLWLKDLDDFEKKLDELDSKEAEDDRKRSSQASKKTA 996

Query: 475  XXXXXXXXXXXXNVVKVTKPKGIAGCMKIQKDSAATDAILSLQEFQGLDAYNFKPSGEKT 654
                           K TK           KD    +   S++    ++    KP G + 
Sbjct: 997  NGFASKPTKKTPQPRKNTKK---------NKDVEPENDKSSMEVENVVEV--VKPKG-RA 1044

Query: 655  GAXXXXXXXXXXXXXXXXYRELLGAYNLIPSGEKTGALETEEPPLPAGKKQNNRKGGAKK 834
            G+                  E L  YN   SGEK+ A+E+EE P+PAGKK  N++ GAKK
Sbjct: 1045 GSKNTQKKAGDENDIPSSLHERLAVYNFESSGEKSEAMESEE-PVPAGKKPINKRAGAKK 1103

Query: 835  --TTVVLDTDSENEVGXXXXXXXFELVQQPAPEALKKEGGRN----------LGTKRTFG 978
              +T+VL+ DS+NE         FE+ Q+  P    K+GGR           L  KR  G
Sbjct: 1104 MSSTIVLEPDSDNE-----DDDNFEVQQKATP---AKKGGRKPAAQNNKAPALPKKRKVG 1155

Query: 979  --QMLLTDMLQPAEGTGTSSPEKKVRKMRESPFNKGKSPSEDLSWGSGS 1119
              Q+LLTDMLQPAE T T SPEKK RKM E          EDLS GS S
Sbjct: 1156 TKQILLTDMLQPAESTAT-SPEKKARKMSEPSL-------EDLSAGSAS 1196


>ref|XP_015952953.1| DNA topoisomerase 2-like [Arachis duranensis]
          Length = 1483

 Score =  191 bits (485), Expect = 2e-49
 Identities = 159/458 (34%), Positives = 219/458 (47%), Gaps = 41/458 (8%)
 Frame = +1

Query: 1    RLEYYEKRRENILSNLKRLLFLLYNIVKFILRDMKRKM-KRHISETDLLMELKRQGFTPM 177
            RLEYY +R++  L NL+R+L  L N V+FIL  +  ++   +  + DLL+ELK++GFTPM
Sbjct: 1018 RLEYYVRRKKYKLDNLERVLLTLDNKVRFILGVVNGEIIVSNRRKADLLIELKQKGFTPM 1077

Query: 178  PREGKSTS---TGANDDNLE-----------ALKDDYEYLLYLPYKAFVIEEVQELLAEM 315
            PR+GKS      G NDDN E           A   DYEYLL +P     +E VQ+LLAE 
Sbjct: 1078 PRKGKSAEPQVAGDNDDNPEEQETESVNVEGARVGDYEYLLSMPIGTLTLESVQKLLAEK 1137

Query: 316  NEKKKQFDVLKTTTAKSLWMKDLNELEKKLDEIYGXXXXXXXXXXXXXXXXXXXXXXXXX 495
            +EK+K++++LK T  KS+WM+DL++LEKKLDE+                           
Sbjct: 1138 DEKEKEYEILKATPPKSMWMEDLDQLEKKLDEL------ETKEAEEERKRSSQANKKTST 1191

Query: 496  XXXXXNVVKVTKPKGIAGCMKIQKDSAATDAILSLQEFQGLDAYNFKPSGEKTGAXXXXX 675
                    K  + K I     I+++  A D   S  E +       KP G + G+     
Sbjct: 1192 RAVTTKASKKPRQKNIKKANVIEEE--AEDISNSSMERENAPEV-AKPKG-RVGSKKEPI 1247

Query: 676  XXXXXXXXXXXYRELLGAYNLIPSGEKTGALETEEPPLPAGKKQNNRKGGAKK---TTVV 846
                        R  L AYN   S         ++      KK+ N++GGAKK   + +V
Sbjct: 1248 KMVDEEIQSLQER--LAAYNFAASAAMQFEQSADD---NVAKKEYNKRGGAKKKASSNLV 1302

Query: 847  LD----TDSENEVGXXXXXXXFELVQQPAPEALKKEGGRNLGTKRT-------------- 972
            LD     + EN+ G        E  Q  APEA+KK+GGR    +                
Sbjct: 1303 LDISGSDNDENDDG------DLERQQPAAPEAVKKKGGRKPAAQNAKKPPATIRKRGASK 1356

Query: 973  -----FGQMLLTDMLQPAEGTGTSSPEKKVRKMRESPFNKGKSPSEDLSWGSGSAKSGSI 1137
                  GQ  +TDML+PAE +G SSPEKKVRKMRESPFNK               KSGS+
Sbjct: 1357 KESELLGQKFITDMLKPAESSGNSSPEKKVRKMRESPFNK---------------KSGSV 1401

Query: 1138 LGRAANRDKSQSEDLSLGSGSATRFPLNFEEDVAEIAP 1251
            L   A ++ + S ++S GS S+     +  E+V E AP
Sbjct: 1402 LSN-AQKEGTGSGEMSFGSASSA--SASIMEEVIEAAP 1436