BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00000018
         (1088 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_013455790.1| nitrilase/nitrile hydratase NIT4A-like prote...   320   e-137
ref|XP_003538102.1| PREDICTED: bifunctional nitrilase/nitrile hy...   314   e-135
dbj|GAU20007.1| hypothetical protein TSUD_273420 [Trifolium subt...   313   e-135
gb|KHN08817.1| Bifunctional nitrilase/nitrile hydratase NIT4A [G...   310   e-134
ref|XP_004506205.1| PREDICTED: bifunctional nitrilase/nitrile hy...   311   e-134
gb|AFK41272.1| unknown [Lotus japonicus]                              305   e-133
ref|XP_007132470.1| hypothetical protein PHAVU_011G096700g [Phas...   313   e-133
ref|XP_014518652.1| bifunctional nitrilase/nitrile hydratase NIT...   312   e-133
ref|XP_020215452.1| bifunctional nitrilase/nitrile hydratase NIT...   312   e-133
ref|NP_001241587.1| uncharacterized protein LOC100810230 [Glycin...   308   e-133
ref|XP_017432152.1| PREDICTED: bifunctional nitrilase/nitrile hy...   311   e-132
gb|KHN21170.1| Bifunctional nitrilase/nitrile hydratase NIT4B [G...   305   e-132
ref|XP_019448859.1| PREDICTED: bifunctional nitrilase/nitrile hy...   316   e-132
gb|PNY04705.1| bifunctional nitrilase nitrile hydratase nit4b-li...   307   e-131
gb|PON78836.1| Nitrilase/cyanide hydratase [Parasponia andersonii]    316   e-131
ref|XP_024158048.1| bifunctional nitrilase/nitrile hydratase NIT...   314   e-130
ref|XP_015953946.1| bifunctional nitrilase/nitrile hydratase NIT...   310   e-130
ref|XP_015872579.1| PREDICTED: bifunctional nitrilase/nitrile hy...   317   e-130
ref|XP_010102024.1| bifunctional nitrilase/nitrile hydratase NIT...   315   e-130
gb|PON65237.1| Nitrilase/cyanide hydratase [Trema orientalis]         313   e-130

>ref|XP_013455790.1| nitrilase/nitrile hydratase NIT4A-like protein [Medicago truncatula]
 gb|KEH29821.1| nitrilase/nitrile hydratase NIT4A-like protein [Medicago truncatula]
          Length = 350

 Score =  320 bits (820), Expect(2) = e-137
 Identities = 148/180 (82%), Positives = 169/180 (93%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV+ETQIGKIG AICWENKMPLLRT +Y+KGVEIYCAPTAD R++
Sbjct: 162  ALERIIWGFGDGSTIPVFETQIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREL 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRRKD+PP+P+YVFEG+E+ LTPDSVVCAGGSVIISPS
Sbjct: 222  WQASMTHIALEGGCFVLSANQFCRRKDYPPAPDYVFEGSEENLTPDSVVCAGGSVIISPS 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGPNYE EALISADLDLGEIARAKFDFDVVGHY+RPE+LSL+V+D P   VT+AS+
Sbjct: 282  GAVLAGPNYEGEALISADLDLGEIARAKFDFDVVGHYARPEVLSLIVKDHPTNPVTFASA 341



 Score =  197 bits (502), Expect(2) = e-137
 Identities = 100/128 (78%), Positives = 110/128 (85%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA VT TPTIN+G + +EVDMGSD NA TVRATVVQAST+FYDTPATLDKAERLLAEAA 
Sbjct: 1   MALVTTTPTINDGPLISEVDMGSDFNALTVRATVVQASTIFYDTPATLDKAERLLAEAAS 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAF+GGYP GS F   IG    KGRE+FRKYH+AAI+VPGPEVDRLA MAG
Sbjct: 61  YGSQLVVFPEAFIGGYPRGSGFGVSIGNRTAKGREDFRKYHSAAIDVPGPEVDRLAVMAG 120

Query: 505 KYKVHLVV 528
           KYKVHLV+
Sbjct: 121 KYKVHLVM 128


>ref|XP_003538102.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Glycine
            max]
 gb|KRH30383.1| hypothetical protein GLYMA_11G180900 [Glycine max]
          Length = 350

 Score =  314 bits (805), Expect(2) = e-135
 Identities = 145/180 (80%), Positives = 164/180 (91%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE++IWG+GD STIPV+ET +GKIG AICWEN+MPLLRT +Y+KGVEIYCAPTAD RDV
Sbjct: 162  ALERVIWGFGDGSTIPVFETPVGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADSRDV 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRRKD+PP PEYVF G E+ LTPDSVVCAGGSVIISPS
Sbjct: 222  WQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPS 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL+V+D P   VT+ S+
Sbjct: 282  GAVLAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLIVKDHPTNPVTFTST 341



 Score =  198 bits (503), Expect(2) = e-135
 Identities = 102/128 (79%), Positives = 109/128 (85%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA VT  PTIN G +  EVDMGSD NA TVRATVVQAST+FYDTPATLDKAERLLAEAA 
Sbjct: 1   MALVTTNPTINEGPLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAAS 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAFVGGYP GS+F   IG    KGREEFRKYH+AAI+VPGPEVDRLAAMAG
Sbjct: 61  YGSQLVVFPEAFVGGYPRGSAFGLSIGNRTAKGREEFRKYHSAAIDVPGPEVDRLAAMAG 120

Query: 505 KYKVHLVV 528
           KYKVHLV+
Sbjct: 121 KYKVHLVM 128


>dbj|GAU20007.1| hypothetical protein TSUD_273420 [Trifolium subterraneum]
          Length = 350

 Score =  313 bits (803), Expect(2) = e-135
 Identities = 145/180 (80%), Positives = 167/180 (92%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE++IWG+GD STIPV+ETQIGKIG AICWENKMPLLRT +Y+KGVEIYCAPTAD R++
Sbjct: 162  ALERVIWGFGDGSTIPVFETQIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREL 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++ HIA EGGCFVLS+NQFCRRKD+PP+PEYVFEG+E+ LTPDSVVCAGGSVIISPS
Sbjct: 222  WQASMAHIAHEGGCFVLSSNQFCRRKDYPPAPEYVFEGSEEDLTPDSVVCAGGSVIISPS 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            GTVLAGPNYE EALI+ADLDLGEIARAKFDFDVVGHY+RPE+LSL+V+D P   VT+ S+
Sbjct: 282  GTVLAGPNYEGEALITADLDLGEIARAKFDFDVVGHYARPEVLSLIVKDHPTIPVTFTSA 341



 Score =  197 bits (501), Expect(2) = e-135
 Identities = 99/128 (77%), Positives = 110/128 (85%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           M+ VT TPTIN+G +  EVDMGSD NA TVRATV+QAST+FYDTPATLDKAERLLAEAA 
Sbjct: 1   MSLVTTTPTINDGPIIAEVDMGSDFNALTVRATVIQASTIFYDTPATLDKAERLLAEAAS 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAF+GGYP GS F   IG    KGRE+FRKYH+AAI+VPGPEVDRLAAMAG
Sbjct: 61  YGSQLVVFPEAFIGGYPRGSGFGVSIGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAG 120

Query: 505 KYKVHLVV 528
           KYKVHLV+
Sbjct: 121 KYKVHLVM 128


>gb|KHN08817.1| Bifunctional nitrilase/nitrile hydratase NIT4A [Glycine soja]
          Length = 350

 Score =  310 bits (794), Expect(2) = e-134
 Identities = 144/180 (80%), Positives = 162/180 (90%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE++IWG+GD STIPV+ET +GKIG AICWEN+MPLLRT +Y+KGVEIYCAPTAD RDV
Sbjct: 162  ALERVIWGFGDGSTIPVFETPVGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADSRDV 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRRKD+PP PEYVF G E+ LTPDSVVCAGGSVIISPS
Sbjct: 222  WQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPS 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL V+D     VT+ S+
Sbjct: 282  GAVLAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLTVKDHQTNPVTFTST 341



 Score =  198 bits (503), Expect(2) = e-134
 Identities = 102/128 (79%), Positives = 109/128 (85%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA VT  PTIN G +  EVDMGSD NA TVRATVVQAST+FYDTPATLDKAERLLAEAA 
Sbjct: 1   MALVTTNPTINEGPLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAAS 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAFVGGYP GS+F   IG    KGREEFRKYH+AAI+VPGPEVDRLAAMAG
Sbjct: 61  YGSQLVVFPEAFVGGYPRGSAFGLSIGNRTAKGREEFRKYHSAAIDVPGPEVDRLAAMAG 120

Query: 505 KYKVHLVV 528
           KYKVHLV+
Sbjct: 121 KYKVHLVM 128


>ref|XP_004506205.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Cicer
            arietinum]
          Length = 377

 Score =  311 bits (797), Expect(2) = e-134
 Identities = 142/180 (78%), Positives = 164/180 (91%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE++IWG+GD STIPV+ETQIGKIG  ICWENKMPLLRT +Y+KGVEIYCAPTAD R++
Sbjct: 189  ALERVIWGFGDGSTIPVFETQIGKIGAVICWENKMPLLRTAMYAKGVEIYCAPTADSREL 248

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQ ++ HIA+EGGCFVLSANQFCRRKD+PP+P+YVFEG E+ LTPDSVVCAGGSVI+SPS
Sbjct: 249  WQPSMIHIALEGGCFVLSANQFCRRKDYPPAPDYVFEGTEEDLTPDSVVCAGGSVIVSPS 308

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGPNYE EALISADLDLG+IARAKFDFDVVGHY RPE+LSL+V+D P   VT+AS+
Sbjct: 309  GAVLAGPNYEGEALISADLDLGDIARAKFDFDVVGHYGRPEVLSLIVKDHPTNPVTFAST 368



 Score =  196 bits (499), Expect(2) = e-134
 Identities = 100/130 (76%), Positives = 111/130 (85%)
 Frame = +1

Query: 139 KHMAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEA 318
           +HMA VT TP IN+G +  EVDMGSD NA TVRATVVQAST+FYDTPATLDKAERLLAEA
Sbjct: 26  QHMAPVTTTPIINDGPLIAEVDMGSDFNAQTVRATVVQASTIFYDTPATLDKAERLLAEA 85

Query: 319 ARNGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAM 498
           A  GSQIVVFPEA++GGYP GS F   +G    KGRE+FRKYH+AAI+VPGPEVDRLAAM
Sbjct: 86  ASYGSQIVVFPEAYIGGYPRGSVFGVSLGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAM 145

Query: 499 AGKYKVHLVV 528
           AGKYKVHLV+
Sbjct: 146 AGKYKVHLVM 155


>gb|AFK41272.1| unknown [Lotus japonicus]
          Length = 346

 Score =  305 bits (781), Expect(2) = e-133
 Identities = 141/179 (78%), Positives = 161/179 (89%)
 Frame = +2

Query: 551  LEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDVW 730
            +E+++WG+GD STIPV+ET +GKIG  ICWEN+MPLLRT +Y+KGVEIYCAPTAD R+VW
Sbjct: 164  MERVMWGFGDGSTIPVFETPLGKIGAVICWENRMPLLRTAMYAKGVEIYCAPTADAREVW 223

Query: 731  QATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPSG 910
            QA++THIA+EGGCFVLSANQFCRRKD+PP PEYVF G E+ LTPDSVVCAGGSVIISP G
Sbjct: 224  QASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPLG 283

Query: 911  TVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
             VLAGPNYE EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL V+D P   VT+AS+
Sbjct: 284  NVLAGPNYEGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLSVKDHPTNPVTFAST 342



 Score =  201 bits (511), Expect(2) = e-133
 Identities = 103/129 (79%), Positives = 114/129 (88%), Gaps = 1/129 (0%)
 Frame = +1

Query: 145 MAFV-TATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAA 321
           MA V T TPTINNG +  EVDMGSD+NA TVRATVVQAST+FYDTPATLDKAERLLAEAA
Sbjct: 1   MALVPTTTPTINNGPLIAEVDMGSDSNAPTVRATVVQASTIFYDTPATLDKAERLLAEAA 60

Query: 322 RNGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMA 501
             GSQ+VVFPEAF+GGYP GS+F  IIGI   KGRE+FRKYH++AI+VPGPEVDRLAAMA
Sbjct: 61  GYGSQLVVFPEAFIGGYPRGSNFGIIIGIRTAKGREDFRKYHSSAIDVPGPEVDRLAAMA 120

Query: 502 GKYKVHLVV 528
           GKYKVHLV+
Sbjct: 121 GKYKVHLVM 129


>ref|XP_007132470.1| hypothetical protein PHAVU_011G096700g [Phaseolus vulgaris]
 gb|ESW04464.1| hypothetical protein PHAVU_011G096700g [Phaseolus vulgaris]
          Length = 350

 Score =  313 bits (801), Expect(2) = e-133
 Identities = 146/180 (81%), Positives = 164/180 (91%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV+ET IGKIG AICWEN+MPLLRT +Y+KGVEIYCAPTAD RDV
Sbjct: 162  ALERIIWGFGDGSTIPVFETPIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADARDV 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRR+D+PP PEYVF G E+ LTPDSVVCAGGSVIISPS
Sbjct: 222  WQASMTHIALEGGCFVLSANQFCRRRDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPS 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL V+D P  +VT+ S+
Sbjct: 282  GAVLAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLTVKDHPTNAVTFTST 341



 Score =  192 bits (487), Expect(2) = e-133
 Identities = 99/128 (77%), Positives = 106/128 (82%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA VTA P IN G +  EVDM SD NA TVRATVVQAST+FYDTPATLDKAERLL EAA 
Sbjct: 1   MALVTAEPIINEGPLFAEVDMASDFNAPTVRATVVQASTIFYDTPATLDKAERLLGEAAG 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
           +GSQ+VVFPEAFVGGYP GS F   IG    KGREEFRKYH+AAI+VPGPEVDRLA MAG
Sbjct: 61  HGSQLVVFPEAFVGGYPRGSDFGVSIGNRTAKGREEFRKYHSAAIDVPGPEVDRLATMAG 120

Query: 505 KYKVHLVV 528
           KYKVHLV+
Sbjct: 121 KYKVHLVM 128


>ref|XP_014518652.1| bifunctional nitrilase/nitrile hydratase NIT4A [Vigna radiata var.
            radiata]
          Length = 350

 Score =  312 bits (799), Expect(2) = e-133
 Identities = 146/180 (81%), Positives = 163/180 (90%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV+ET IGKIG AICWEN+MPLLRT +Y+KGVEIYCAPTAD RDV
Sbjct: 162  ALERIIWGFGDGSTIPVFETPIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADARDV 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRR+D+PP PEYVF G E+ LTPDSVVCAGGSVIISPS
Sbjct: 222  WQASMTHIALEGGCFVLSANQFCRRRDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPS 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL V+D P   VT+ S+
Sbjct: 282  GAVLAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLTVKDHPTNPVTFTSA 341



 Score =  192 bits (488), Expect(2) = e-133
 Identities = 98/128 (76%), Positives = 106/128 (82%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA VT  PTIN G +  EVDM SD NA TVRATV+QAST+FYDTPATLDKAERLL EAA 
Sbjct: 1   MALVTTEPTINEGPLFAEVDMASDFNAPTVRATVIQASTIFYDTPATLDKAERLLGEAAG 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
           +GSQ+VVFPEAFVGGYP GS F   IG    KGREEFRKYH+AAI+VPGPEVDRLA MAG
Sbjct: 61  HGSQLVVFPEAFVGGYPRGSDFGVSIGNRTAKGREEFRKYHSAAIDVPGPEVDRLATMAG 120

Query: 505 KYKVHLVV 528
           KYKVHLV+
Sbjct: 121 KYKVHLVM 128


>ref|XP_020215452.1| bifunctional nitrilase/nitrile hydratase NIT4A [Cajanus cajan]
 gb|KYP68697.1| hypothetical protein KK1_022337 [Cajanus cajan]
          Length = 350

 Score =  312 bits (799), Expect(2) = e-133
 Identities = 144/180 (80%), Positives = 164/180 (91%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE++IWG+GD STIPV+ET IGK+G AICWEN+MPLLRT +Y+KGVEIYCAPTAD R+V
Sbjct: 162  ALERVIWGFGDGSTIPVFETPIGKLGAAICWENRMPLLRTAMYAKGVEIYCAPTADSREV 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRRKD+PP PEYVF G E+ LTPDSVVCAGGS IISPS
Sbjct: 222  WQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYVFAGIEEDLTPDSVVCAGGSAIISPS 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL+V+D P   VT+AS+
Sbjct: 282  GAVLAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLIVKDHPTNPVTFAST 341



 Score =  192 bits (487), Expect(2) = e-133
 Identities = 98/128 (76%), Positives = 108/128 (84%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA VT  PT+N G +  EVDMGSD NA TVRATVVQAST+FYDTPATLDKAER LAEAA 
Sbjct: 1   MALVTTNPTMNEGPLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERFLAEAAG 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAFVGGYP GS+F   IG    KGREEFR+YH+AAI+VPGPEVDRLAAMAG
Sbjct: 61  YGSQLVVFPEAFVGGYPRGSTFGVSIGNRTPKGREEFRRYHSAAIDVPGPEVDRLAAMAG 120

Query: 505 KYKVHLVV 528
           KYKV+LV+
Sbjct: 121 KYKVYLVM 128


>ref|NP_001241587.1| uncharacterized protein LOC100810230 [Glycine max]
 gb|ACU18374.1| unknown [Glycine max]
 gb|KRH25286.1| hypothetical protein GLYMA_12G092300 [Glycine max]
          Length = 350

 Score =  308 bits (788), Expect(2) = e-133
 Identities = 143/180 (79%), Positives = 161/180 (89%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE++IWG+GD STIPV+ET +GKIG AICWEN+MPLLRT +Y+KGVEIYCAPTAD RDV
Sbjct: 162  ALERVIWGFGDGSTIPVFETPVGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADARDV 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRR+D+PP PEYVF G E  LTPDSVVCAGGSVIISP 
Sbjct: 222  WQASMTHIALEGGCFVLSANQFCRRRDYPPPPEYVFAGTEVDLTPDSVVCAGGSVIISPL 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL V+D P   VT+ S+
Sbjct: 282  GAVLAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLTVKDHPTNPVTFTST 341



 Score =  196 bits (498), Expect(2) = e-133
 Identities = 101/128 (78%), Positives = 108/128 (84%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA VT  PTIN G +  EVDMGSD NA TVRATVVQAST+FYDTPATLDKAERLLAEA  
Sbjct: 1   MALVTTNPTINEGPLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEATS 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAFVGGYP GS+F   IG    KGREEFRKYH+AAI+VPGPEVDRLAAMAG
Sbjct: 61  YGSQLVVFPEAFVGGYPRGSAFGLSIGNRTVKGREEFRKYHSAAIDVPGPEVDRLAAMAG 120

Query: 505 KYKVHLVV 528
           KYKVHLV+
Sbjct: 121 KYKVHLVM 128


>ref|XP_017432152.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Vigna
            angularis]
 gb|KOM50515.1| hypothetical protein LR48_Vigan08g134200 [Vigna angularis]
 dbj|BAT90374.1| hypothetical protein VIGAN_06160900 [Vigna angularis var. angularis]
          Length = 350

 Score =  311 bits (797), Expect(2) = e-132
 Identities = 144/180 (80%), Positives = 163/180 (90%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV+ET IGKIG AICWEN+MPLLRT +Y+KG+EIYCAPTAD RDV
Sbjct: 162  ALERIIWGFGDGSTIPVFETPIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADARDV 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRR+D+PP PEYVF G E+ LTPDSVVCAGGSVIISPS
Sbjct: 222  WQASMTHIALEGGCFVLSANQFCRRRDYPPPPEYVFAGTEEDLTPDSVVCAGGSVIISPS 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G +LAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL V+D P   VT+ S+
Sbjct: 282  GAILAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLTVKDHPTNPVTFTST 341



 Score =  192 bits (488), Expect(2) = e-132
 Identities = 98/128 (76%), Positives = 106/128 (82%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA VT  PTIN G +  EVDM SD NA TVRATV+QAST+FYDTPATLDKAERLL EAA 
Sbjct: 1   MALVTTEPTINEGPLFAEVDMASDFNAPTVRATVIQASTIFYDTPATLDKAERLLGEAAG 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
           +GSQ+VVFPEAFVGGYP GS F   IG    KGREEFRKYH+AAI+VPGPEVDRLA MAG
Sbjct: 61  HGSQLVVFPEAFVGGYPRGSDFGVSIGNRTAKGREEFRKYHSAAIDVPGPEVDRLATMAG 120

Query: 505 KYKVHLVV 528
           KYKVHLV+
Sbjct: 121 KYKVHLVM 128


>gb|KHN21170.1| Bifunctional nitrilase/nitrile hydratase NIT4B [Glycine soja]
          Length = 350

 Score =  305 bits (780), Expect(2) = e-132
 Identities = 142/180 (78%), Positives = 160/180 (88%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE++IWG+GD STIPV+ET +GKIG AICWEN+MPLLRT +Y+KGVEIYCAPTAD RDV
Sbjct: 162  ALERVIWGFGDGSTIPVFETPVGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADARDV 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRR+D+PP PEYVF G E  LTPDSVVCAGGSVIISP 
Sbjct: 222  WQASMTHIALEGGCFVLSANQFCRRRDYPPPPEYVFAGTEVDLTPDSVVCAGGSVIISPL 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL V+D     VT+ S+
Sbjct: 282  GAVLAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLTVKDHQTNPVTFTST 341



 Score =  196 bits (499), Expect(2) = e-132
 Identities = 101/128 (78%), Positives = 108/128 (84%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA VT  PTIN G +  EVDMGSD NA TVRATVVQAST+FYDTPATLDKAERLLAEA  
Sbjct: 1   MALVTTNPTINEGPLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEATS 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAFVGGYP GS+F   IG    KGREEFRKYH+AAI+VPGPEVDRLAAMAG
Sbjct: 61  YGSQLVVFPEAFVGGYPRGSAFGLSIGNRTAKGREEFRKYHSAAIDVPGPEVDRLAAMAG 120

Query: 505 KYKVHLVV 528
           KYKVHLV+
Sbjct: 121 KYKVHLVM 128


>ref|XP_019448859.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A isoform X1
            [Lupinus angustifolius]
 sp|Q5QGZ8.1|NRL4A_LUPAN RecName: Full=Bifunctional nitrilase/nitrile hydratase NIT4A;
            Short=LaNIT4A; AltName: Full=3-cyanoalanine hydratase;
            AltName: Full=Cyanoalanine nitrilase A
 gb|AAT36331.1| nitrilase 4A [Lupinus angustifolius]
 gb|ABB51979.1| nitrilase 4A [Lupinus angustifolius]
 gb|OIW08474.1| hypothetical protein TanjilG_03150 [Lupinus angustifolius]
          Length = 349

 Score =  316 bits (809), Expect(2) = e-132
 Identities = 148/180 (82%), Positives = 165/180 (91%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV+ET IGKIG AICWENKMPLLRT +Y+KGVEIYCAPTAD R+V
Sbjct: 161  ALERIIWGFGDGSTIPVFETPIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREV 220

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRR+D+PP PEYVFEG E+ LTPDSVVCAGGSVIISPS
Sbjct: 221  WQASMTHIALEGGCFVLSANQFCRRRDYPPPPEYVFEGTEENLTPDSVVCAGGSVIISPS 280

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGP+YE EALISADLDLGEIARAKFDFDVVGHYSRPE+LSLVV+D P   VT+ S+
Sbjct: 281  GAVLAGPSYEGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLVVKDHPTNPVTFTSA 340



 Score =  184 bits (468), Expect(2) = e-132
 Identities = 95/128 (74%), Positives = 110/128 (85%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA VT TPT+N+G +  EV+M SD NA TVRATVVQAST+FYDTPATLDKAERLLAEAA 
Sbjct: 1   MALVT-TPTVNDGPLFAEVNMSSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAAS 59

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            G+QIVVFPEAF+GGYP GS+F   IG    KG+E+FRKYH+AAI+VPGPEVDRLAA+AG
Sbjct: 60  YGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEDFRKYHSAAIDVPGPEVDRLAALAG 119

Query: 505 KYKVHLVV 528
           KYKV+LV+
Sbjct: 120 KYKVYLVM 127


>gb|PNY04705.1| bifunctional nitrilase nitrile hydratase nit4b-like protein
            [Trifolium pratense]
          Length = 428

 Score =  307 bits (786), Expect(2) = e-131
 Identities = 142/176 (80%), Positives = 163/176 (92%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE++IWG+GD STIPV+ETQIGKIG AICWENKMPLLRT +Y+KGVEIYCAPTAD R++
Sbjct: 164  ALERVIWGFGDGSTIPVFETQIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREL 223

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++ HIA EGGCFVLSANQFCRRKD+PP+PEYVFEG+E+ LTPDSVVCAGGSVI+SPS
Sbjct: 224  WQASMAHIAHEGGCFVLSANQFCRRKDYPPAPEYVFEGSEEDLTPDSVVCAGGSVIMSPS 283

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVT 1075
            G VLAGPNYE EALI+ADLDLGEIARAKFDFDVVGHY+RPE+LSL+V+D P   V+
Sbjct: 284  GAVLAGPNYEGEALITADLDLGEIARAKFDFDVVGHYARPEVLSLIVKDHPTIPVS 339



 Score =  192 bits (487), Expect(2) = e-131
 Identities = 96/124 (77%), Positives = 107/124 (86%)
 Frame = +1

Query: 157 TATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAARNGSQ 336
           T TPTIN+G +  EVDMGSD NA TVRATV+QAST+FYDTPATLDKAERLLAEAA  G+Q
Sbjct: 7   TPTPTINDGPIIAEVDMGSDFNALTVRATVIQASTIFYDTPATLDKAERLLAEAATYGAQ 66

Query: 337 IVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAGKYKV 516
           +VVFPEAF+GGYP GS F   IG    KGRE+FRKYH+AAI+VPGPEVDRLAAMAGKYKV
Sbjct: 67  LVVFPEAFIGGYPRGSGFGVSIGNRTAKGREDFRKYHSAAIDVPGPEVDRLAAMAGKYKV 126

Query: 517 HLVV 528
           HLV+
Sbjct: 127 HLVM 130


>gb|PON78836.1| Nitrilase/cyanide hydratase [Parasponia andersonii]
          Length = 347

 Score =  316 bits (810), Expect(2) = e-131
 Identities = 148/180 (82%), Positives = 165/180 (91%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV+ET IGKIG AICWEN+MPLLRT +Y+KGVEIYCAPTAD R+V
Sbjct: 161  ALERIIWGFGDGSTIPVFETPIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADAREV 220

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRRKD+PP PEYVF G E +LTPDSVVCAGGSVIISPS
Sbjct: 221  WQASMTHIAVEGGCFVLSANQFCRRKDYPPPPEYVFSGTEGELTPDSVVCAGGSVIISPS 280

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            GTVLAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL+V+D P   VT+ SS
Sbjct: 281  GTVLAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLIVRDHPTNPVTFTSS 340



 Score =  181 bits (458), Expect(2) = e-131
 Identities = 94/128 (73%), Positives = 109/128 (85%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA V + P +N+  +  EVDMG+D++ASTVRATVVQASTV+YDTPATLDKAERLLAEAA 
Sbjct: 1   MALVPSAP-LNDAPLFAEVDMGADSSASTVRATVVQASTVYYDTPATLDKAERLLAEAAG 59

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAFVGGYP GS+F   IG    KG+EEFRKYHA+AI+VPGPEVD LAAMAG
Sbjct: 60  YGSQLVVFPEAFVGGYPRGSNFGVTIGNRTPKGKEEFRKYHASAIDVPGPEVDLLAAMAG 119

Query: 505 KYKVHLVV 528
           KYKV+LV+
Sbjct: 120 KYKVYLVM 127


>ref|XP_024158048.1| bifunctional nitrilase/nitrile hydratase NIT4A [Rosa chinensis]
 gb|PRQ32497.1| putative hydrolase, 3-cyanoalanine hydratase [Rosa chinensis]
          Length = 351

 Score =  314 bits (805), Expect(2) = e-130
 Identities = 142/180 (78%), Positives = 167/180 (92%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV++T IGKIG AICWENKMPLLRT +Y+KG+EIYCAPTAD RD+
Sbjct: 162  ALERIIWGFGDGSTIPVFDTPIGKIGAAICWENKMPLLRTAMYAKGIEIYCAPTADSRDL 221

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRRKD+PP PEY+F G E++LTPDSVVCAGGSVIISPS
Sbjct: 222  WQASMTHIALEGGCFVLSANQFCRRKDYPPPPEYIFSGTEEELTPDSVVCAGGSVIISPS 281

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            GT+LAGPNY+ EALISADLD+G+IARAKFDFDVVGHY+RPE+LSL+V+D PA  VT+ S+
Sbjct: 282  GTILAGPNYDGEALISADLDIGDIARAKFDFDVVGHYARPEVLSLIVRDHPANPVTFTSA 341



 Score =  182 bits (462), Expect(2) = e-130
 Identities = 94/128 (73%), Positives = 104/128 (81%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA V ++  +N   V  EVDMG D++  TVRATVVQASTVFYDTPATLDKAERLLAEAA 
Sbjct: 1   MALVPSSAPVNESPVITEVDMGGDDSTPTVRATVVQASTVFYDTPATLDKAERLLAEAAG 60

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAFVGGYP GS+F   IG    KG+EEFRKYHAAAI VPGPEVDRLA+MAG
Sbjct: 61  YGSQLVVFPEAFVGGYPRGSNFGVAIGNRTAKGKEEFRKYHAAAITVPGPEVDRLASMAG 120

Query: 505 KYKVHLVV 528
           KYKV LV+
Sbjct: 121 KYKVFLVM 128


>ref|XP_015953946.1| bifunctional nitrilase/nitrile hydratase NIT4A [Arachis duranensis]
          Length = 352

 Score =  310 bits (795), Expect(2) = e-130
 Identities = 145/180 (80%), Positives = 165/180 (91%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV+ET IGKIG AICWEN+MPLLRT +Y+KGVEIYCAPTAD RDV
Sbjct: 163  ALERIIWGFGDGSTIPVFETPIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADARDV 222

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRRKD+PP+PEY F G E++LTPD+VVCAGGSVIISPS
Sbjct: 223  WQASMTHIALEGGCFVLSANQFCRRKDYPPAPEYEFCGTEEELTPDAVVCAGGSVIISPS 282

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            G VLAGPNY+ EALISADLDLGEIAR+KFDFDVVGHYSRPE+LSLVV+D P   VT+ S+
Sbjct: 283  GAVLAGPNYDGEALISADLDLGEIARSKFDFDVVGHYSRPEVLSLVVKDHPTNPVTFTST 342



 Score =  186 bits (471), Expect(2) = e-130
 Identities = 97/129 (75%), Positives = 110/129 (85%), Gaps = 1/129 (0%)
 Frame = +1

Query: 145 MAFVTATPT-INNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAA 321
           MA VT + + +N+G +  EVDMGSD NA TVRATVVQAST+FYDTPATLDKAERLLAEAA
Sbjct: 1   MALVTTSSSGVNDGPLFAEVDMGSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAA 60

Query: 322 RNGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMA 501
             GSQ+VVFPEAFVGGYP GS+F   IG    KGRE+FRKYH+AAI+VPGPEVDRLAAMA
Sbjct: 61  GYGSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGREDFRKYHSAAIDVPGPEVDRLAAMA 120

Query: 502 GKYKVHLVV 528
           GKYKV+LV+
Sbjct: 121 GKYKVYLVM 129


>ref|XP_015872579.1| PREDICTED: bifunctional nitrilase/nitrile hydratase NIT4A [Ziziphus
            jujuba]
          Length = 351

 Score =  317 bits (811), Expect(2) = e-130
 Identities = 147/180 (81%), Positives = 165/180 (91%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV+ET IGKIG AICWENKMPLLRT +Y+KG+EIYCAPTAD RDV
Sbjct: 161  ALERIIWGFGDGSTIPVFETPIGKIGAAICWENKMPLLRTAMYAKGIEIYCAPTADARDV 220

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRRKD+PP PEYVF G E++L+PDSVVCAGGSVIISPS
Sbjct: 221  WQASMTHIAVEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEELSPDSVVCAGGSVIISPS 280

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            GTVLAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+L L+V+D P   VT+ SS
Sbjct: 281  GTVLAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLGLIVRDHPTSPVTFTSS 340



 Score =  179 bits (455), Expect(2) = e-130
 Identities = 93/128 (72%), Positives = 108/128 (84%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           M+ V + P IN   +  EVDMGSD++A TVRATVVQAST+FYDTPATLDKAERLLAEAA 
Sbjct: 1   MSLVPSVP-INESPLFAEVDMGSDSSAPTVRATVVQASTIFYDTPATLDKAERLLAEAAG 59

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            G+Q+VVFPEAFVGGYP GS+F   IG    KG+EEFRKYHA+AI+VPGPEVDRLAAMAG
Sbjct: 60  YGAQLVVFPEAFVGGYPRGSNFGVTIGNRTVKGKEEFRKYHASAIDVPGPEVDRLAAMAG 119

Query: 505 KYKVHLVV 528
           KY+V+LV+
Sbjct: 120 KYRVYLVM 127


>ref|XP_010102024.1| bifunctional nitrilase/nitrile hydratase NIT4A [Morus notabilis]
 gb|EXB91244.1| Bifunctional nitrilase/nitrile hydratase NIT4A [Morus notabilis]
          Length = 349

 Score =  315 bits (806), Expect(2) = e-130
 Identities = 146/180 (81%), Positives = 164/180 (91%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV+ET IGKIG AICWEN+MPLLRT +Y+KG+EIYCAPTAD R+V
Sbjct: 161  ALERIIWGFGDGSTIPVFETPIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADAREV 220

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EGGCFVLSANQFCRRKD+PP PEYVF G E+ LTPDSVVCAGGSVIISPS
Sbjct: 221  WQASMTHIAVEGGCFVLSANQFCRRKDYPPPPEYVFSGTEEDLTPDSVVCAGGSVIISPS 280

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            GTVLAGPNY+ EALISADLDLGEI RAKFDFDVVGHYSRPE+LSL+V+D P   VT+ SS
Sbjct: 281  GTVLAGPNYDGEALISADLDLGEIVRAKFDFDVVGHYSRPEILSLIVRDHPTNLVTFTSS 340



 Score =  181 bits (458), Expect(2) = e-130
 Identities = 91/128 (71%), Positives = 110/128 (85%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA V +  T+N G +  EVDMG+D+++ TVRATVVQAST+FYDTPATLDKAERLLAEAA 
Sbjct: 1   MALVPSA-TVNEGPIFAEVDMGADSSSPTVRATVVQASTIFYDTPATLDKAERLLAEAAV 59

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAF+GGYP GS+F + IG    KG+EEFR+YHA+AI+VPGPEV+RLAAMAG
Sbjct: 60  YGSQLVVFPEAFIGGYPRGSNFGATIGNRTAKGKEEFRRYHASAIDVPGPEVERLAAMAG 119

Query: 505 KYKVHLVV 528
           KYKV+LV+
Sbjct: 120 KYKVYLVM 127


>gb|PON65237.1| Nitrilase/cyanide hydratase [Trema orientalis]
          Length = 347

 Score =  313 bits (803), Expect(2) = e-130
 Identities = 147/180 (81%), Positives = 164/180 (91%)
 Frame = +2

Query: 548  ALEKIIWGYGDASTIPVYETQIGKIGGAICWENKMPLLRTILYSKGVEIYCAPTADDRDV 727
            ALE+IIWG+GD STIPV+ET IGKIG AICWEN+MPLLRT +Y+KGVEIYCAPTAD R+V
Sbjct: 161  ALERIIWGFGDGSTIPVFETPIGKIGAAICWENRMPLLRTAMYAKGVEIYCAPTADAREV 220

Query: 728  WQATVTHIAIEGGCFVLSANQFCRRKDFPPSPEYVFEGAEKKLTPDSVVCAGGSVIISPS 907
            WQA++THIA+EG CFVLSANQFCRRKD+PP PEYVF G E +LTPDSVVCAGGSVIISPS
Sbjct: 221  WQASMTHIALEGACFVLSANQFCRRKDYPPPPEYVFSGTEGELTPDSVVCAGGSVIISPS 280

Query: 908  GTVLAGPNYEEEALISADLDLGEIARAKFDFDVVGHYSRPELLSLVVQDKPAKSVTYASS 1087
            GTVLAGPNY+ EALISADLDLGEIARAKFDFDVVGHYSRPE+LSL+V+D P   VT+ SS
Sbjct: 281  GTVLAGPNYDGEALISADLDLGEIARAKFDFDVVGHYSRPEVLSLIVRDHPTNPVTFTSS 340



 Score =  181 bits (459), Expect(2) = e-130
 Identities = 94/128 (73%), Positives = 109/128 (85%)
 Frame = +1

Query: 145 MAFVTATPTINNGGVSNEVDMGSDNNASTVRATVVQASTVFYDTPATLDKAERLLAEAAR 324
           MA V + P +N+  +  EVDMG+D++A +VRATVVQASTVFYDTPATLDKAERLLAEAA 
Sbjct: 1   MALVPSAP-LNDAPLFAEVDMGADSSAPSVRATVVQASTVFYDTPATLDKAERLLAEAAG 59

Query: 325 NGSQIVVFPEAFVGGYPHGSSFDSIIGIPKDKGREEFRKYHAAAINVPGPEVDRLAAMAG 504
            GSQ+VVFPEAFVGGYP GS+F   IG    KG+EEFRKYHA+AI+VPGPEVDRLAAMAG
Sbjct: 60  YGSQLVVFPEAFVGGYPRGSNFGVTIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAG 119

Query: 505 KYKVHLVV 528
           KYKV+LV+
Sbjct: 120 KYKVYLVM 127