BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00029706
         (1104 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_015939341.1| E3 ubiquitin-protein ligase ATL4, partial [A...   254   3e-79
ref|XP_016174278.1| E3 ubiquitin-protein ligase ATL4-like [Arach...   253   4e-78
ref|XP_004510135.1| PREDICTED: E3 ubiquitin-protein ligase ATL4-...   249   8e-77
ref|XP_019412674.1| PREDICTED: E3 ubiquitin-protein ligase ATL4-...   244   1e-76
ref|XP_003530107.1| PREDICTED: E3 ubiquitin-protein ligase ATL4-...   248   3e-76
ref|XP_020208961.1| E3 ubiquitin-protein ligase ATL4-like [Cajan...   243   1e-75
ref|XP_003521039.1| PREDICTED: E3 ubiquitin-protein ligase ATL4-...   246   2e-75
ref|XP_007134368.1| hypothetical protein PHAVU_010G041800g [Phas...   233   5e-71
dbj|BAT96886.1| hypothetical protein VIGAN_09020100 [Vigna angul...   228   9e-70
ref|XP_014516066.1| E3 ubiquitin-protein ligase ATL4 [Vigna radi...   228   9e-70
ref|XP_017441861.1| PREDICTED: E3 ubiquitin-protein ligase ATL4 ...   228   9e-70
dbj|GAU49733.1| hypothetical protein TSUD_43200 [Trifolium subte...   230   1e-69
ref|XP_003626798.1| RING zinc finger protein, putative [Medicago...   226   6e-68
gb|PNX86784.1| E3 ubiquitin-protein ligase atl4-like protein, pa...   224   2e-67
gb|PNX77735.1| E3 ubiquitin-protein ligase atl4-like protein [Tr...   224   2e-67
ref|XP_020203196.1| E3 ubiquitin-protein ligase ATL4-like [Cajan...   205   6e-60
ref|XP_004488154.1| PREDICTED: E3 ubiquitin-protein ligase ATL4-...   201   3e-58
ref|XP_007052410.2| PREDICTED: E3 ubiquitin-protein ligase ATL4 ...   188   5e-58
gb|EOX96567.1| Toxicos en levadura 4, putative [Theobroma cacao]      188   5e-58
gb|PNY00796.1| E3 ubiquitin-protein ligase atl4-like protein [Tr...   199   9e-58

>ref|XP_015939341.1| E3 ubiquitin-protein ligase ATL4, partial [Arachis duranensis]
          Length = 286

 Score =  254 bits (648), Expect = 3e-79
 Identities = 135/221 (61%), Positives = 161/221 (72%), Gaps = 24/221 (10%)
 Frame = -2

Query: 857 ANPSAATTPIITSGRRVSPEIP-------SSVIDSLPLFXXXXXXXXXXXXXADCAVCLS 699
           A PS++ TPI TS RR+SPE+P       S +IDSLPLF              DCAVCLS
Sbjct: 9   ATPSSSATPIFTSSRRISPEVPQTSSTSYSYIIDSLPLFTFSSVTRRSSAA--DCAVCLS 66

Query: 698 KFHNNDLLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPS----V 531
           KF N+DLLRLLP+CCHAFHA+CIDTWL+SNLSCPLCRS+I A++SDL KVLRS S     
Sbjct: 67  KFRNDDLLRLLPICCHAFHAECIDTWLQSNLSCPLCRSAIVATESDLGKVLRSSSSTAGS 126

Query: 530 NSFRIEIGNVSSRGTNPDAN---------GESRSRSYSIGSFEYFVEEESEIPFSEAHRR 378
           +SFR+EIGNVS RGT PD +         G +RSRSYSIGSFEYFV+E+SE+PFS AHRR
Sbjct: 127 DSFRLEIGNVSRRGTAPDGSAAVSGESRAGGARSRSYSIGSFEYFVDEDSEVPFSHAHRR 186

Query: 377 SISDRKEVSASRESPAMSNEAALA----GTRNWLKEYVDRV 267
           S SD+K++ AS E+PA  +EA LA    G R+WLKEY+DRV
Sbjct: 187 STSDQKDIPASAEAPASQHEANLAGDVGGVRSWLKEYMDRV 227



 Score = 65.1 bits (157), Expect = 4e-08
 Identities = 31/37 (83%), Positives = 34/37 (91%), Gaps = 1/37 (2%)
 Frame = -1

Query: 210 GSSRRSDVVPI-PVEYDVEGNRIGEEISEMFRWLSGV 103
           GSSRRSDVVP+   EYD+EGNRIGEEI+EMFRWLSGV
Sbjct: 250 GSSRRSDVVPVVAAEYDIEGNRIGEEITEMFRWLSGV 286


>ref|XP_016174278.1| E3 ubiquitin-protein ligase ATL4-like [Arachis ipaensis]
          Length = 356

 Score =  253 bits (646), Expect = 4e-78
 Identities = 145/269 (53%), Positives = 173/269 (64%), Gaps = 27/269 (10%)
 Frame = -2

Query: 992 RSSPLQNLSPXXXXXXXXXXXXXXXXXAXXXXXXXXXXXXXXXXN---ANPSAATTPIIT 822
           RSSPLQNLSP                 A                +   A PS++ TPI T
Sbjct: 31  RSSPLQNLSPSILIIVTVLAVTVIVSLALCFLLRHINRRCLRRLSRFSATPSSSATPIFT 90

Query: 821 SGRRVSPEIP-------SSVIDSLPLFXXXXXXXXXXXXXADCAVCLSKFHNNDLLRLLP 663
           S RR+SPE+P       S +IDSLPLF              DCAVCLSKF N+DLLRLLP
Sbjct: 91  SSRRISPEVPQNSSTSYSYIIDSLPLFTFSSVTRRSSAA--DCAVCLSKFRNDDLLRLLP 148

Query: 662 LCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPS----VNSFRIEIGNVSS 495
           +CCHAFHA+CIDTWL+SNLSCPLCRS+I A++SDL KV+RS S     +SFR+EIGNVS 
Sbjct: 149 ICCHAFHAECIDTWLQSNLSCPLCRSAIVATESDLGKVVRSSSSTSGSDSFRLEIGNVSR 208

Query: 494 RGTNPDAN---------GESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKEVSASR 342
           RGT PD +         G +RSRSYSIGSFEYFV+E+SE+PFS AHRRS SD+K++ AS 
Sbjct: 209 RGTAPDGSAAVSGESRAGGARSRSYSIGSFEYFVDEDSEVPFSHAHRRSTSDQKDIPASA 268

Query: 341 ESPAMSNEAALA----GTRNWLKEYVDRV 267
           E+PA  +EA LA    G R+WLKEY+DRV
Sbjct: 269 EAPASQHEANLAGEVGGVRSWLKEYMDRV 297



 Score = 65.1 bits (157), Expect = 6e-08
 Identities = 31/37 (83%), Positives = 34/37 (91%), Gaps = 1/37 (2%)
 Frame = -1

Query: 210 GSSRRSDVVPI-PVEYDVEGNRIGEEISEMFRWLSGV 103
           GSSRRSDVVP+   EYD+EGNRIGEEI+EMFRWLSGV
Sbjct: 320 GSSRRSDVVPVVAAEYDIEGNRIGEEITEMFRWLSGV 356


>ref|XP_004510135.1| PREDICTED: E3 ubiquitin-protein ligase ATL4-like [Cicer arietinum]
          Length = 348

 Score =  249 bits (637), Expect = 8e-77
 Identities = 138/250 (55%), Positives = 164/250 (65%), Gaps = 7/250 (2%)
 Frame = -2

Query: 995 HRSSPLQNLSPXXXXXXXXXXXXXXXXXAXXXXXXXXXXXXXXXXNANPSAATTPIITSG 816
           H SSPLQN+SP                 A                +++P A   P++ SG
Sbjct: 46  HSSSPLQNISPSILIIVTVLAVTVIVSFALCFLLRHLNRRCLRRLSSDPDAFPPPVVISG 105

Query: 815 RRVSPEI--PSSVIDSLPLFXXXXXXXXXXXXXADCAVCLSKFHNNDLLRLLPLCCHAFH 642
           R  SPEI  PS +IDSLPLF             ADCAVCLSKFHNNDLLRLLP CCHAFH
Sbjct: 106 R-TSPEIIPPSDLIDSLPLFTFSSVTRRSSTAAADCAVCLSKFHNNDLLRLLPRCCHAFH 164

Query: 641 ADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPSVNSFRIEIGNVSSRGTNPDANGES 462
            DCIDTWLRSNLSCPLCRS++F SDSDLAK+LRSPS +SFRIEIGNVS RGT PDA G+ 
Sbjct: 165 VDCIDTWLRSNLSCPLCRSAVFPSDSDLAKILRSPSSDSFRIEIGNVSRRGTIPDAAGD- 223

Query: 461 RSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRK-EVSASRESPAMSNEAALAG----TR 297
             RS+S+GSF+Y+VEE SE+ F+  HRRSISD K ++  S +SPA++    LAG    + 
Sbjct: 224 -GRSFSMGSFDYYVEEPSEVSFTHDHRRSISDHKVDIPTSDDSPAINETVVLAGEVGSSG 282

Query: 296 NWLKEYVDRV 267
            WLK+YVDR+
Sbjct: 283 FWLKDYVDRL 292



 Score = 61.2 bits (147), Expect = 1e-06
 Identities = 30/41 (73%), Positives = 35/41 (85%), Gaps = 1/41 (2%)
 Frame = -1

Query: 222 KVFTGSSRRSDVVPIPVEYD-VEGNRIGEEISEMFRWLSGV 103
           + F GSSRR+DVV + V+YD VE NRIGEEI+EMFRWLSGV
Sbjct: 308 RFFGGSSRRNDVVQVAVDYDDVESNRIGEEITEMFRWLSGV 348


>ref|XP_019412674.1| PREDICTED: E3 ubiquitin-protein ligase ATL4-like [Lupinus
           angustifolius]
 gb|OIW17631.1| hypothetical protein TanjilG_28981 [Lupinus angustifolius]
          Length = 350

 Score =  244 bits (624), Expect(2) = 1e-76
 Identities = 131/216 (60%), Positives = 154/216 (71%), Gaps = 22/216 (10%)
 Frame = -2

Query: 848 SAATTPIITSGRRVSPEI-PSSVIDSLPLFXXXXXXXXXXXXXADCAVCLSKFHNNDLLR 672
           SAA TPI TS RR+SPEI PSS+IDSLPLF             ADCAVCLSKF ++DLLR
Sbjct: 81  SAAATPIFTSSRRISPEITPSSIIDSLPLFTFSSVTRRSATGAADCAVCLSKFQDHDLLR 140

Query: 671 LLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPS---VNSFRIEIGNV 501
           LLPLCCHAFHA+CIDTWL+SNLSCPLCRS+I A+D+DLAK+LRS S    +SFR+EIGNV
Sbjct: 141 LLPLCCHAFHAECIDTWLQSNLSCPLCRSAIVAADNDLAKILRSSSSAGSDSFRVEIGNV 200

Query: 500 SSRGT--------------NPDANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDR 363
           S RGT              +   +G + +RSYSIGSFEY V EESE+PFS AHRRS+SD+
Sbjct: 201 SRRGTAAATAPEDSVSSTADDARSGGAGTRSYSIGSFEYLVNEESEVPFSNAHRRSVSDQ 260

Query: 362 KEVSASRESPAMSNEAAL----AGTRNWLKEYVDRV 267
           K++    E     NEA L    AG RNWLK+YVDR+
Sbjct: 261 KDIPIPAEYSVTQNEAFLAGDVAGVRNWLKDYVDRI 296



 Score = 72.0 bits (175), Expect(2) = 1e-76
 Identities = 32/38 (84%), Positives = 36/38 (94%)
 Frame = -1

Query: 216 FTGSSRRSDVVPIPVEYDVEGNRIGEEISEMFRWLSGV 103
           F GSSRRSDVVP+ V+YD+EGNRIGEEISEMFRW+SGV
Sbjct: 313 FGGSSRRSDVVPVSVDYDIEGNRIGEEISEMFRWISGV 350


>ref|XP_003530107.1| PREDICTED: E3 ubiquitin-protein ligase ATL4-like [Glycine max]
 gb|KRH48901.1| hypothetical protein GLYMA_07G120300 [Glycine max]
          Length = 359

 Score =  248 bits (634), Expect = 3e-76
 Identities = 141/264 (53%), Positives = 167/264 (63%), Gaps = 22/264 (8%)
 Frame = -2

Query: 992 RSSPLQNLSPXXXXXXXXXXXXXXXXXAXXXXXXXXXXXXXXXXNAN---PSAATTPIIT 822
           RSSPLQNLSP                 A                +++   PSAA TPI  
Sbjct: 37  RSSPLQNLSPSILIIVTVLAVTVIVSLALCFLLRHLNRRCLRRFSSSSAAPSAAATPIFA 96

Query: 821 SGRRVSPEI-------PSSVIDSLPLFXXXXXXXXXXXXXADCAVCLSKFHNNDLLRLLP 663
           S RR+SPEI        +SVID+LPLF              DCAVCLSKFH++DLLRLLP
Sbjct: 97  SSRRISPEILHSSASASASVIDTLPLFTFSSVTRRSAAVSGDCAVCLSKFHHHDLLRLLP 156

Query: 662 LCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSP----SVNSFRIEIGNVSS 495
           LCCHAFHA+CIDTWL+SNLSCPLCRS+I A DSDLAK+LR P    S +SFR+E+GN+S 
Sbjct: 157 LCCHAFHAECIDTWLQSNLSCPLCRSTIVADDSDLAKILRPPSSAGSSDSFRLELGNISR 216

Query: 494 RGTNPDANGES----RSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKEVSASRESPAM 327
           RGT+  A G S     SRSYSIGSFEY +++ESE+PFS A RRS+ D KE  A  E+P  
Sbjct: 217 RGTDGAAEGGSVARGGSRSYSIGSFEYLIDDESEVPFSHARRRSVDDAKEFPAPAEAPVS 276

Query: 326 SNEAALA----GTRNWLKEYVDRV 267
            NEA+LA    G R+WLKEY+DRV
Sbjct: 277 HNEASLAGEVGGVRSWLKEYMDRV 300



 Score = 60.8 bits (146), Expect = 1e-06
 Identities = 29/36 (80%), Positives = 31/36 (86%), Gaps = 1/36 (2%)
 Frame = -1

Query: 207 SSRRSDVVPIPV-EYDVEGNRIGEEISEMFRWLSGV 103
           S RRSD+VP+   EYD EGNRIGEEISEMFRWLSGV
Sbjct: 324 SDRRSDIVPVAAPEYDAEGNRIGEEISEMFRWLSGV 359


>ref|XP_020208961.1| E3 ubiquitin-protein ligase ATL4-like [Cajanus cajan]
          Length = 344

 Score =  243 bits (621), Expect(2) = 1e-75
 Identities = 140/259 (54%), Positives = 167/259 (64%), Gaps = 18/259 (6%)
 Frame = -2

Query: 992 RSSPLQNLSPXXXXXXXXXXXXXXXXXAXXXXXXXXXXXXXXXXNAN---PSAATTPIIT 822
           RSSPLQNLSP                 +                +++   PSAA TP+  
Sbjct: 31  RSSPLQNLSPSILIIVTVLAVTVIVSLSLCFLLRYLSRRCLQRFSSSSAAPSAAATPVFA 90

Query: 821 SGRRVSPEIP---SSVIDSLPLFXXXXXXXXXXXXXA-DCAVCLSKFHNNDLLRLLPLCC 654
           + RR+SPE+P   +SVIDSLPLF             A DCAVCLSKFHN+DLLRLLPLCC
Sbjct: 91  ASRRISPEVPHSSASVIDSLPLFTFSSITRRSSAAVAADCAVCLSKFHNHDLLRLLPLCC 150

Query: 653 HAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSP-----SVNSFRIEIGNVSSRG 489
           HAFHA CIDTWL++NL+CPLCRS+I A+DSDLAK+LR P     S +SFR+EIGN+S RG
Sbjct: 151 HAFHAQCIDTWLQNNLTCPLCRSAIVAADSDLAKILRQPSSTAGSSDSFRLEIGNISRRG 210

Query: 488 TNPDA--NGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKEVSASRESPAMSNEA 315
           T   A   G SRSRSYSIGSFEY V++ESE+PFS A RRS+ D KE  A+ ES    NEA
Sbjct: 211 TEGAAATRGSSRSRSYSIGSFEYLVDDESEVPFSHARRRSVDDAKEFPAAAES---QNEA 267

Query: 314 AL----AGTRNWLKEYVDR 270
            L    AG R+WLK+Y+DR
Sbjct: 268 TLAGEVAGVRSWLKDYMDR 286



 Score = 70.1 bits (170), Expect(2) = 1e-75
 Identities = 32/36 (88%), Positives = 34/36 (94%)
 Frame = -1

Query: 210 GSSRRSDVVPIPVEYDVEGNRIGEEISEMFRWLSGV 103
           GSSRRSDVVP+  EYD+EGNRIGEEISEMFRWLSGV
Sbjct: 309 GSSRRSDVVPVAAEYDLEGNRIGEEISEMFRWLSGV 344


>ref|XP_003521039.1| PREDICTED: E3 ubiquitin-protein ligase ATL4-like [Glycine max]
 gb|KRH66395.1| hypothetical protein GLYMA_03G103800 [Glycine max]
          Length = 359

 Score =  246 bits (629), Expect = 2e-75
 Identities = 141/263 (53%), Positives = 166/263 (63%), Gaps = 21/263 (7%)
 Frame = -2

Query: 992 RSSPLQNLSPXXXXXXXXXXXXXXXXXAXXXXXXXXXXXXXXXXNAN--PSAATTPIITS 819
           RSSPLQNLSP                 A                +++  PSAA TPI  S
Sbjct: 37  RSSPLQNLSPSILIIVTVLAVTVIVSLALCFLLRHLNRRCLRRFSSSAPPSAAATPIFAS 96

Query: 818 GRRVSPEI-------PSSVIDSLPLFXXXXXXXXXXXXXADCAVCLSKFHNNDLLRLLPL 660
            RR+SPEI        +SVID+LP+F              DCAVCLSKFH++DLLRLLPL
Sbjct: 97  SRRISPEILHSSASASASVIDTLPVFTFSSVTRRSSSVAGDCAVCLSKFHHHDLLRLLPL 156

Query: 659 CCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSP----SVNSFRIEIGNVSSR 492
           CCHAFHA+CIDTWL+SNLSCPLCRS+I A DSDLAK+LR P    S +SFR+E+GN+S R
Sbjct: 157 CCHAFHAECIDTWLQSNLSCPLCRSAIVADDSDLAKILRPPSSGGSSDSFRLELGNISRR 216

Query: 491 GTNPDANGES----RSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKEVSASRESPAMS 324
           GT+  A G S     SRSYSIGSFEY V++ESE+PFS A RRS+ D KE  A  E+P   
Sbjct: 217 GTDGAAEGGSVARGGSRSYSIGSFEYLVDDESEVPFSHASRRSVDDAKEFPAPAEAPVRH 276

Query: 323 NEAALA----GTRNWLKEYVDRV 267
           NEA LA    G R+WLK+YVDRV
Sbjct: 277 NEANLAGEVGGVRSWLKDYVDRV 299



 Score = 62.8 bits (151), Expect = 3e-07
 Identities = 31/36 (86%), Positives = 32/36 (88%), Gaps = 1/36 (2%)
 Frame = -1

Query: 207 SSRRSDVVPIPV-EYDVEGNRIGEEISEMFRWLSGV 103
           S RRSDVVP+   EYDVEGNRIGEEISEMFRWLSGV
Sbjct: 324 SGRRSDVVPVAASEYDVEGNRIGEEISEMFRWLSGV 359


>ref|XP_007134368.1| hypothetical protein PHAVU_010G041800g [Phaseolus vulgaris]
 gb|ESW06362.1| hypothetical protein PHAVU_010G041800g [Phaseolus vulgaris]
          Length = 358

 Score =  233 bits (593), Expect(2) = 5e-71
 Identities = 125/212 (58%), Positives = 153/212 (72%), Gaps = 19/212 (8%)
 Frame = -2

Query: 848 SAATTPIITSGRRVSPEI-------PSSVIDSLPLFXXXXXXXXXXXXXADCAVCLSKFH 690
           SA+ TPI +S RR+SPEI        +SVID+LPLF             ADCAVCLSKFH
Sbjct: 88  SASATPIFSSSRRISPEIVHSSSAAAASVIDTLPLFTFSSVTRRSASAAADCAVCLSKFH 147

Query: 689 NNDLLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSP-----SVNS 525
           ++DLLRLLPLCCHAFHA+CIDTWL+SNLSCPLCRS I A++SDLAK+LR P     S +S
Sbjct: 148 HHDLLRLLPLCCHAFHAECIDTWLQSNLSCPLCRSPIAAAESDLAKILRPPSSAAGSSDS 207

Query: 524 FRIEIGNVSSRGTN--PDANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKEV- 354
           FR+E+GN+S RG    P A  +SRSRSYSIGSFEY V+++SE+ FS A RRS  D KE  
Sbjct: 208 FRLELGNISRRGAEGAPAARSDSRSRSYSIGSFEYLVDDDSEVAFSHARRRSEDDAKEFP 267

Query: 353 SASRESPAMSNEAAL----AGTRNWLKEYVDR 270
           +A  ++PA  NEA+L    AG R+WLK+Y+DR
Sbjct: 268 AAEAQTPANHNEASLAGEVAGVRSWLKDYMDR 299



 Score = 65.5 bits (158), Expect(2) = 5e-71
 Identities = 32/37 (86%), Positives = 34/37 (91%), Gaps = 1/37 (2%)
 Frame = -1

Query: 210 GSSRRSDVVPI-PVEYDVEGNRIGEEISEMFRWLSGV 103
           GSSRRSDVVP+   EYD+EGNRIGEEISEMFRWLSGV
Sbjct: 322 GSSRRSDVVPVVAAEYDLEGNRIGEEISEMFRWLSGV 358


>dbj|BAT96886.1| hypothetical protein VIGAN_09020100 [Vigna angularis var.
           angularis]
          Length = 362

 Score =  228 bits (582), Expect(2) = 9e-70
 Identities = 123/216 (56%), Positives = 153/216 (70%), Gaps = 23/216 (10%)
 Frame = -2

Query: 848 SAATTPIITSGRRVSPEI---------PSSVIDSLPLFXXXXXXXXXXXXXADCAVCLSK 696
           SA+ TPI +S RR+SPEI          +SVID+LPLF             ADCAVCLSK
Sbjct: 88  SASATPIFSSSRRISPEIVLSSSSAAVAASVIDTLPLFTFSSVTRRSSSAAADCAVCLSK 147

Query: 695 FHNNDLLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSP-----SV 531
           FH++DLLRLLPLCCHAFHA+CIDTWL+SNLSCPLCRS I A++SD+AK+LR P     S 
Sbjct: 148 FHHHDLLRLLPLCCHAFHAECIDTWLQSNLSCPLCRSPIAAAESDMAKILRPPSSTAGSS 207

Query: 530 NSFRIEIGNVSSRGTN----PDANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDR 363
           +SFR+E+GN+S RG        A G+SRSRSYSIGSF+Y V++ESE+ FS A RRS  D 
Sbjct: 208 DSFRLELGNISRRGAEGAAATAARGDSRSRSYSIGSFDYLVDDESEVAFSHARRRSEDDA 267

Query: 362 KEVSASR-ESPAMSNEAAL----AGTRNWLKEYVDR 270
           KE  A+  ++P+  NEA+L    AG R+WLK+Y+DR
Sbjct: 268 KEFLANETQTPSTHNEASLASEVAGVRSWLKDYMDR 303



 Score = 65.5 bits (158), Expect(2) = 9e-70
 Identities = 32/37 (86%), Positives = 34/37 (91%), Gaps = 1/37 (2%)
 Frame = -1

Query: 210 GSSRRSDVVPI-PVEYDVEGNRIGEEISEMFRWLSGV 103
           GSSRRSDVVP+   EYD+EGNRIGEEISEMFRWLSGV
Sbjct: 326 GSSRRSDVVPVVAAEYDLEGNRIGEEISEMFRWLSGV 362


>ref|XP_014516066.1| E3 ubiquitin-protein ligase ATL4 [Vigna radiata var. radiata]
          Length = 362

 Score =  228 bits (582), Expect(2) = 9e-70
 Identities = 123/216 (56%), Positives = 153/216 (70%), Gaps = 23/216 (10%)
 Frame = -2

Query: 848 SAATTPIITSGRRVSPEI---------PSSVIDSLPLFXXXXXXXXXXXXXADCAVCLSK 696
           SA+ TPI +S RR+SPEI          +SVID+LPLF             ADCAVCLSK
Sbjct: 88  SASATPIFSSSRRISPEIVHSSSTAAVAASVIDTLPLFTFSSVTRRSSSAAADCAVCLSK 147

Query: 695 FHNNDLLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSP-----SV 531
           FH++DLLRLLPLCCHAFHA+CIDTWL+SNLSCPLCRS I A++SD+AK+LR P     S 
Sbjct: 148 FHHHDLLRLLPLCCHAFHAECIDTWLQSNLSCPLCRSPISAAESDIAKILRPPSSTAGSS 207

Query: 530 NSFRIEIGNVSSRGTN----PDANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDR 363
           +SFR+E+GN+S RG        A G+SRSRSYSIGSF+Y V++ESE+ FS A RRS  D 
Sbjct: 208 DSFRLELGNISRRGAEGAAATAARGDSRSRSYSIGSFDYLVDDESEVAFSHARRRSEDDA 267

Query: 362 KEVSASR-ESPAMSNEAAL----AGTRNWLKEYVDR 270
           KE  A+  ++P+  NEA+L    AG R+WLK+Y+DR
Sbjct: 268 KEFLATETQTPSTHNEASLASEVAGVRSWLKDYMDR 303



 Score = 65.5 bits (158), Expect(2) = 9e-70
 Identities = 32/37 (86%), Positives = 34/37 (91%), Gaps = 1/37 (2%)
 Frame = -1

Query: 210 GSSRRSDVVPI-PVEYDVEGNRIGEEISEMFRWLSGV 103
           GSSRRSDVVP+   EYD+EGNRIGEEISEMFRWLSGV
Sbjct: 326 GSSRRSDVVPVVAAEYDLEGNRIGEEISEMFRWLSGV 362


>ref|XP_017441861.1| PREDICTED: E3 ubiquitin-protein ligase ATL4 [Vigna angularis]
 gb|KOM58557.1| hypothetical protein LR48_Vigan11g159100 [Vigna angularis]
          Length = 362

 Score =  228 bits (582), Expect(2) = 9e-70
 Identities = 123/216 (56%), Positives = 153/216 (70%), Gaps = 23/216 (10%)
 Frame = -2

Query: 848 SAATTPIITSGRRVSPEI---------PSSVIDSLPLFXXXXXXXXXXXXXADCAVCLSK 696
           SA+ TPI +S RR+SPEI          +SVID+LPLF             ADCAVCLSK
Sbjct: 88  SASATPIFSSSRRISPEIVLSSSSAAVAASVIDTLPLFTFSSVTRRPSSAAADCAVCLSK 147

Query: 695 FHNNDLLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSP-----SV 531
           FH++DLLRLLPLCCHAFHA+CIDTWL+SNLSCPLCRS I A++SD+AK+LR P     S 
Sbjct: 148 FHHHDLLRLLPLCCHAFHAECIDTWLQSNLSCPLCRSPIAAAESDMAKILRPPSSTAGSS 207

Query: 530 NSFRIEIGNVSSRGTN----PDANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDR 363
           +SFR+E+GN+S RG        A G+SRSRSYSIGSF+Y V++ESE+ FS A RRS  D 
Sbjct: 208 DSFRLELGNISRRGAEGAAATAARGDSRSRSYSIGSFDYLVDDESEVAFSHARRRSEDDA 267

Query: 362 KEVSASR-ESPAMSNEAAL----AGTRNWLKEYVDR 270
           KE  A+  ++P+  NEA+L    AG R+WLK+Y+DR
Sbjct: 268 KEFLANETQTPSTHNEASLASEVAGVRSWLKDYMDR 303



 Score = 65.5 bits (158), Expect(2) = 9e-70
 Identities = 32/37 (86%), Positives = 34/37 (91%), Gaps = 1/37 (2%)
 Frame = -1

Query: 210 GSSRRSDVVPI-PVEYDVEGNRIGEEISEMFRWLSGV 103
           GSSRRSDVVP+   EYD+EGNRIGEEISEMFRWLSGV
Sbjct: 326 GSSRRSDVVPVVAAEYDLEGNRIGEEISEMFRWLSGV 362


>dbj|GAU49733.1| hypothetical protein TSUD_43200 [Trifolium subterraneum]
          Length = 317

 Score =  230 bits (587), Expect = 1e-69
 Identities = 123/204 (60%), Positives = 147/204 (72%), Gaps = 9/204 (4%)
 Frame = -2

Query: 851 PSAATTPIITSGRRVSPEI-PSSVIDSLPLFXXXXXXXXXXXXXA-DCAVCLSKFHNNDL 678
           P+  T    +   R+SPEI P SVIDSLPLF             A DCAVCLSKF N+DL
Sbjct: 61  PTTTTIESTSHSNRISPEIVPPSVIDSLPLFTYSSISRRSSAVTAADCAVCLSKFRNSDL 120

Query: 677 LRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPSVNSFRIEIGNVS 498
           LR LPLCCHAFHA+CIDTWLRSNLSCPLCRSSIF SDSDLAK+LRS S +SFR+EIGNVS
Sbjct: 121 LRSLPLCCHAFHAECIDTWLRSNLSCPLCRSSIFPSDSDLAKILRSNSSDSFRVEIGNVS 180

Query: 497 SRGTNPD-----ANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKEVSA--SRE 339
            RGTN +       G   SRSYSIGSF+Y+V E SE+PFS+ HRRSISD+KE+    S +
Sbjct: 181 RRGTNSNNPELAGGGGGDSRSYSIGSFDYYVVEPSEVPFSQDHRRSISDQKEIHIPFSDD 240

Query: 338 SPAMSNEAALAGTRNWLKEYVDRV 267
           S  ++    ++G+ +WLK+YVDR+
Sbjct: 241 SSVVTVAGEVSGSGHWLKDYVDRL 264


>ref|XP_003626798.1| RING zinc finger protein, putative [Medicago truncatula]
 gb|AET01274.1| RING zinc finger protein, putative [Medicago truncatula]
          Length = 316

 Score =  226 bits (575), Expect = 6e-68
 Identities = 134/256 (52%), Positives = 160/256 (62%), Gaps = 13/256 (5%)
 Frame = -2

Query: 995 HRSSPLQNLSPXXXXXXXXXXXXXXXXXAXXXXXXXXXXXXXXXXNANPSAATT--PIIT 822
           HRSSPLQN+SP                                    NPS  TT  P  T
Sbjct: 11  HRSSPLQNISPSILIIITILAVTVIVSLLICFLLRHLNCHRLRR---NPSPTTTEPPPHT 67

Query: 821 SGRRVSPEI-PSSVIDSLPLFXXXXXXXXXXXXXA-DCAVCLSKFHNNDLLRLLPLCCHA 648
             RR+SPE  P SVIDSLPLF             A DCAVCLSKF N+DLLR LPLCCHA
Sbjct: 68  HSRRISPETTPPSVIDSLPLFTFSSISRRSSAVTAADCAVCLSKFRNSDLLRSLPLCCHA 127

Query: 647 FHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPSVNSFRIEIGNVSSRGT---NPD 477
           FHA+CIDTWLRSNLSCPLCR+SI  SDSDLAK+LRS S +SFR++IGN+S RG    NPD
Sbjct: 128 FHAECIDTWLRSNLSCPLCRASILPSDSDLAKILRSTSSDSFRVDIGNISRRGNNLPNPD 187

Query: 476 A-NGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKEVSAS-RESPAMSNE----A 315
           A      SRSYSIGSFEY+V E SE+ F++ HRRS+SD+K++     E  +M++E     
Sbjct: 188 AVTTGGDSRSYSIGSFEYYVVEPSEVSFTQDHRRSVSDQKDIHIPVSEDSSMTHENTIAG 247

Query: 314 ALAGTRNWLKEYVDRV 267
            +AG+ +WLK+YVDR+
Sbjct: 248 EVAGSGHWLKDYVDRL 263



 Score = 58.2 bits (139), Expect = 9e-06
 Identities = 29/36 (80%), Positives = 31/36 (86%)
 Frame = -1

Query: 210 GSSRRSDVVPIPVEYDVEGNRIGEEISEMFRWLSGV 103
           GSSRR +    PV+YDVEGNRIGEEISEMFRWLSGV
Sbjct: 285 GSSRRIE----PVDYDVEGNRIGEEISEMFRWLSGV 316


>gb|PNX86784.1| E3 ubiquitin-protein ligase atl4-like protein, partial [Trifolium
           pratense]
          Length = 317

 Score =  224 bits (572), Expect = 2e-67
 Identities = 122/206 (59%), Positives = 150/206 (72%), Gaps = 9/206 (4%)
 Frame = -2

Query: 857 ANPSAATTPIITS--GRRVSPEIPS-SVIDSLPLFXXXXXXXXXXXXXA-DCAVCLSKFH 690
           A+P++ TT   +S    ++SPEI S SVIDSLPLF             A DCAVCLSKF 
Sbjct: 60  ADPTSTTTTTESSHHNNQISPEIISPSVIDSLPLFTYSSISRRSSAVTAADCAVCLSKFR 119

Query: 689 NNDLLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPSVNSFRIEI 510
           N+DLLR LPLCCHAFH+DCIDTWLRSNLSCPLCRSS+F SDSDLAK+LRS S  SFR+EI
Sbjct: 120 NSDLLRSLPLCCHAFHSDCIDTWLRSNLSCPLCRSSVFPSDSDLAKILRSNSSESFRVEI 179

Query: 509 GNVSSRG---TNPDANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKE--VSAS 345
           GNVS RG   TNP+  G   SRSYSIGSF+Y V E SE+ F++ HRRS+SD+KE  +  S
Sbjct: 180 GNVSRRGTNSTNPELAGGGDSRSYSIGSFDYHVVEPSEVSFTQDHRRSVSDQKEIHIPIS 239

Query: 344 RESPAMSNEAALAGTRNWLKEYVDRV 267
            +S  ++    ++G+ +WLK+YVDR+
Sbjct: 240 DDSSVVTIAGEVSGSGHWLKDYVDRL 265


>gb|PNX77735.1| E3 ubiquitin-protein ligase atl4-like protein [Trifolium pratense]
 gb|PNX81141.1| E3 ubiquitin-protein ligase atl4-like protein [Trifolium pratense]
          Length = 318

 Score =  224 bits (572), Expect = 2e-67
 Identities = 122/206 (59%), Positives = 150/206 (72%), Gaps = 9/206 (4%)
 Frame = -2

Query: 857 ANPSAATTPIITS--GRRVSPEIPS-SVIDSLPLFXXXXXXXXXXXXXA-DCAVCLSKFH 690
           A+P++ TT   +S    ++SPEI S SVIDSLPLF             A DCAVCLSKF 
Sbjct: 60  ADPTSTTTTTESSHHNNQISPEIISPSVIDSLPLFTYSSISRRSSAVTAADCAVCLSKFR 119

Query: 689 NNDLLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPSVNSFRIEI 510
           N+DLLR LPLCCHAFH+DCIDTWLRSNLSCPLCRSS+F SDSDLAK+LRS S  SFR+EI
Sbjct: 120 NSDLLRSLPLCCHAFHSDCIDTWLRSNLSCPLCRSSVFPSDSDLAKILRSNSSESFRVEI 179

Query: 509 GNVSSRG---TNPDANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKE--VSAS 345
           GNVS RG   TNP+  G   SRSYSIGSF+Y V E SE+ F++ HRRS+SD+KE  +  S
Sbjct: 180 GNVSRRGTNSTNPELAGGGDSRSYSIGSFDYHVVEPSEVSFTQDHRRSVSDQKEIHIPIS 239

Query: 344 RESPAMSNEAALAGTRNWLKEYVDRV 267
            +S  ++    ++G+ +WLK+YVDR+
Sbjct: 240 DDSSVVTIAGEVSGSGHWLKDYVDRL 265


>ref|XP_020203196.1| E3 ubiquitin-protein ligase ATL4-like [Cajanus cajan]
          Length = 321

 Score =  205 bits (522), Expect = 6e-60
 Identities = 113/209 (54%), Positives = 141/209 (67%), Gaps = 15/209 (7%)
 Frame = -2

Query: 848 SAATTPIITSGRRVSPEIPSS-VIDSLPLFXXXXXXXXXXXXXAD--CAVCLSKFHNNDL 678
           S +   + +S  R+SPE  SS V DSLP F              D  CAVCLSKF  +DL
Sbjct: 64  SPSIAEVFSSANRISPETASSSVADSLPTFAFSSVTRHGGGGSGDGDCAVCLSKFEQHDL 123

Query: 677 LRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPSV---NSFRIEIG 507
           LRLLPLCCHAFHA+CIDTWLRSNL+CPLCRS++  S+SDLAKV RS SV   +SFR+EIG
Sbjct: 124 LRLLPLCCHAFHAECIDTWLRSNLTCPLCRSAVVGSESDLAKVFRSSSVAGGDSFRLEIG 183

Query: 506 NVSS--RGTNPDA-NGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKEVSASRES 336
           N+SS  RG    A  GE+R RSYS+G+FEYF++EE+E+ FS AHRRS+S  K+     E 
Sbjct: 184 NISSSRRGAATSAGGGETRGRSYSVGAFEYFIDEEAEVAFSYAHRRSVSGEKDDGDPAEP 243

Query: 335 -PAMSNEAALAGTRN-----WLKEYVDRV 267
            P+++ E    G+ N     WLK+YVDR+
Sbjct: 244 LPSLAGEVERGGSSNNDSNSWLKDYVDRL 272


>ref|XP_004488154.1| PREDICTED: E3 ubiquitin-protein ligase ATL4-like [Cicer arietinum]
          Length = 313

 Score =  201 bits (510), Expect = 3e-58
 Identities = 114/206 (55%), Positives = 146/206 (70%), Gaps = 12/206 (5%)
 Frame = -2

Query: 854 NPSAATTPIITSGRRVSPEIPSS-VIDSLPLFXXXXXXXXXXXXXA-DCAVCLSKFHNND 681
           +PS++TT   TSG RVSPEI SS V+DSLP+F             + DCAVCLSKF  +D
Sbjct: 60  SPSSSTTS--TSGNRVSPEITSSSVVDSLPMFSFSSITRRSSSAASGDCAVCLSKFEQHD 117

Query: 680 LLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPSV---NSFRIEI 510
           LLRLLPLC HAFH +CIDTWLRSNL+CPLCRS++ AS+S+L K+ +  S    NSFR+EI
Sbjct: 118 LLRLLPLCHHAFHVECIDTWLRSNLTCPLCRSAVVASESELMKICQPSSASSGNSFRLEI 177

Query: 509 GNVSS--RGTNPDAN-GESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKE----VS 351
           GNVSS  RGT    N GE+  RSYS+G+FEYFV+EE+EIPF + +RR +S  K+    ++
Sbjct: 178 GNVSSSRRGTAASNNVGETDRRSYSVGAFEYFVDEEAEIPFGQTNRRIVSGEKDDAPAMA 237

Query: 350 ASRESPAMSNEAALAGTRNWLKEYVD 273
            + E P +S +A L G  +WLK+YVD
Sbjct: 238 VAVEVPVVS-QANLIGEGSWLKDYVD 262


>ref|XP_007052410.2| PREDICTED: E3 ubiquitin-protein ligase ATL4 isoform X1 [Theobroma
           cacao]
 ref|XP_017970246.1| PREDICTED: E3 ubiquitin-protein ligase ATL4 isoform X2 [Theobroma
           cacao]
          Length = 350

 Score =  188 bits (478), Expect(2) = 5e-58
 Identities = 104/209 (49%), Positives = 135/209 (64%), Gaps = 15/209 (7%)
 Frame = -2

Query: 848 SAATTPIITSGRRVSPEIP--SSVIDSLPLFXXXXXXXXXXXXXA---DCAVCLSKFHNN 684
           S  T+   T+  RVSPE    +S++DSLP+F                 DCAVCLSKF ++
Sbjct: 89  STITSAAATASHRVSPEESQTASLLDSLPVFTFSSIIRRSNNDSTVSGDCAVCLSKFESH 148

Query: 683 DLLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPS---------- 534
           D LRLLPLCCHAFHA CIDTWL SN +CPLCRS +FAS+SDL KVL   S          
Sbjct: 149 DQLRLLPLCCHAFHAHCIDTWLASNQTCPLCRSPLFASESDLMKVLLQSSNSAAAIGSGG 208

Query: 533 VNSFRIEIGNVSSRGTNPDANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKEV 354
            +SFR+EIG++S R   P +    + RSYSIGSF+Y VEEESE+  ++ HRR++SD+++V
Sbjct: 209 SDSFRLEIGSISRR--QPGSESGEQRRSYSIGSFDYVVEEESEVTRNQTHRRTVSDKEDV 266

Query: 353 SASRESPAMSNEAALAGTRNWLKEYVDRV 267
           +A   +   S  A +A  R+WLKEYVDR+
Sbjct: 267 AAPVAASEASLAAEVATGRSWLKEYVDRL 295



 Score = 66.2 bits (160), Expect(2) = 5e-58
 Identities = 30/40 (75%), Positives = 34/40 (85%)
 Frame = -1

Query: 222 KVFTGSSRRSDVVPIPVEYDVEGNRIGEEISEMFRWLSGV 103
           + FTGSSRRSD+  + V+YDVE NRIGEEISEMFRW SGV
Sbjct: 311 RFFTGSSRRSDISGVAVDYDVEANRIGEEISEMFRWFSGV 350


>gb|EOX96567.1| Toxicos en levadura 4, putative [Theobroma cacao]
          Length = 350

 Score =  188 bits (478), Expect(2) = 5e-58
 Identities = 104/209 (49%), Positives = 135/209 (64%), Gaps = 15/209 (7%)
 Frame = -2

Query: 848 SAATTPIITSGRRVSPEIP--SSVIDSLPLFXXXXXXXXXXXXXA---DCAVCLSKFHNN 684
           S  T+   T+  RVSPE    +S++DSLP+F                 DCAVCLSKF ++
Sbjct: 89  STITSAAATASHRVSPEESQTASLLDSLPVFTFSSIIRRSNNDSTVSGDCAVCLSKFESH 148

Query: 683 DLLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPS---------- 534
           D LRLLPLCCHAFHA CIDTWL SN +CPLCRS +FAS+SDL KVL   S          
Sbjct: 149 DQLRLLPLCCHAFHAHCIDTWLASNQTCPLCRSPLFASESDLMKVLLQSSNSAAAIGSGG 208

Query: 533 VNSFRIEIGNVSSRGTNPDANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRKEV 354
            +SFR+EIG++S R   P +    + RSYSIGSF+Y VEEESE+  ++ HRR++SD+++V
Sbjct: 209 SDSFRLEIGSISRR--QPGSESGEQRRSYSIGSFDYVVEEESEVTRNQTHRRTVSDKEDV 266

Query: 353 SASRESPAMSNEAALAGTRNWLKEYVDRV 267
           +A   +   S  A +A  R+WLKEYVDR+
Sbjct: 267 AAPVAASEASLAAEVATGRSWLKEYVDRL 295



 Score = 66.2 bits (160), Expect(2) = 5e-58
 Identities = 30/40 (75%), Positives = 34/40 (85%)
 Frame = -1

Query: 222 KVFTGSSRRSDVVPIPVEYDVEGNRIGEEISEMFRWLSGV 103
           + FTGSSRRSD+  + V+YDVE NRIGEEISEMFRW SGV
Sbjct: 311 RFFTGSSRRSDISGVAVDYDVEANRIGEEISEMFRWFSGV 350


>gb|PNY00796.1| E3 ubiquitin-protein ligase atl4-like protein [Trifolium pratense]
          Length = 307

 Score =  199 bits (506), Expect = 9e-58
 Identities = 106/211 (50%), Positives = 143/211 (67%), Gaps = 14/211 (6%)
 Frame = -2

Query: 857 ANPSAAT--TPIITSGRRVSPEIP---SSVIDSLPLFXXXXXXXXXXXXXA-DCAVCLSK 696
           ++PS++T  +   +SG R+SPE     SS++DSLPLF             + DCAVCLSK
Sbjct: 49  SSPSSSTVFSSSSSSGNRISPETETTSSSIVDSLPLFTFSSITRRSSSVVSLDCAVCLSK 108

Query: 695 FHNNDLLRLLPLCCHAFHADCIDTWLRSNLSCPLCRSSIFASDSDLAKVLRSPSV---NS 525
           F   DLLRLLPLCCHAFH +CID WLRSNL+CPLCRS++  S+S+L K+ R  S    NS
Sbjct: 109 FEQTDLLRLLPLCCHAFHTECIDAWLRSNLTCPLCRSAVVVSESELMKIFRPSSASSGNS 168

Query: 524 FRIEIGNVSSR-----GTNPDANGESRSRSYSIGSFEYFVEEESEIPFSEAHRRSISDRK 360
           FR+EIGNVS+R       + + +GE+  R YS+G+FEYFV+EE+EIPF   +RR +S  K
Sbjct: 169 FRLEIGNVSNRRGDGTAASNNVSGETDRRLYSVGAFEYFVDEEAEIPFGHTNRRIVSGEK 228

Query: 359 EVSASRESPAMSNEAALAGTRNWLKEYVDRV 267
           + S + E P +S +A+L G  +WLK+YVD +
Sbjct: 229 DDSPASEVPVVS-QASLVGEGSWLKDYVDNL 258