BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00026245
         (347 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004498560.1| PREDICTED: DNA topoisomerase 3-alpha isoform...    91   1e-18
gb|PNX79216.1| DNA topoisomerase 1-like protein, partial [Trifol...    88   4e-18
gb|PNY10518.1| DNA topoisomerase 1-like protein [Trifolium prate...    82   3e-16
dbj|GAU15218.1| hypothetical protein TSUD_09560 [Trifolium subte...    75   2e-13
dbj|GAU15221.1| hypothetical protein TSUD_09580 [Trifolium subte...    73   2e-12
dbj|GAU15220.1| hypothetical protein TSUD_09590 [Trifolium subte...    73   2e-12
ref|XP_013442265.1| DNA topoisomerase I, bacterial protein [Medi...    72   2e-12
ref|XP_013465712.1| DNA topoisomerase I, bacterial protein [Medi...    72   2e-12
ref|XP_013442263.1| DNA topoisomerase I, bacterial protein [Medi...    72   2e-12
ref|XP_013465711.1| DNA topoisomerase I, bacterial protein [Medi...    72   2e-12
ref|XP_013442264.1| DNA topoisomerase I, bacterial protein [Medi...    72   2e-12
ref|XP_003588648.2| DNA topoisomerase I, bacterial protein [Medi...    68   7e-11

>ref|XP_004498560.1| PREDICTED: DNA topoisomerase 3-alpha isoform X2 [Cicer arietinum]
 ref|XP_004498561.1| PREDICTED: DNA topoisomerase 3-alpha isoform X1 [Cicer arietinum]
          Length = 1047

 Score = 90.5 bits (223), Expect = 1e-18
 Identities = 56/102 (54%), Positives = 68/102 (66%), Gaps = 2/102 (1%)
 Frame = +2

Query: 47  QLRAFQNPLNSLHLQPHTNLSIGIGNSVQRQNRFPFRCSFGGIS--ISHVANAKSVNGFG 220
           QLR+F NPL S    P+ NL+    N+V R++RFPFR SF  I+   + + +  SVNGFG
Sbjct: 24  QLRSFHNPLIS---PPYVNLT---KNTVNRRSRFPFR-SFAVINHEFTTLKSVTSVNGFG 76

Query: 221 FAFHGNGIGRSLLFSSRAVDSKRKKPVKTVAVRGKDDKDVSS 346
           + F  NG+GR L FSS    SKRKK VK VAV GKDDKDVSS
Sbjct: 77  YPFSANGVGRCLPFSSGTEKSKRKKTVKAVAVPGKDDKDVSS 118


>gb|PNX79216.1| DNA topoisomerase 1-like protein, partial [Trifolium pratense]
          Length = 362

 Score = 87.8 bits (216), Expect = 4e-18
 Identities = 56/103 (54%), Positives = 68/103 (66%), Gaps = 3/103 (2%)
 Frame = +2

Query: 47  QLRAFQNPLNSLHLQPHTNLSIGIGNSVQRQNRFPFRCSFG-GISISHVANAKSVNGFGF 223
           QLR+  NPL S HL    NL+    N+VQ +NRF FR SFG   + + + +  SV+GF  
Sbjct: 25  QLRSLHNPLISPHLPSLVNLTT---NAVQHRNRFSFR-SFGVTTNFNTLKSVTSVSGFSL 80

Query: 224 AFHGNGIGRSLLFSSRAVD--SKRKKPVKTVAVRGKDDKDVSS 346
            F  NG+GRSL FSS A +  SKRKK VK VAVRGKD+KDVSS
Sbjct: 81  PFRENGVGRSLRFSSTAPEKKSKRKKTVKAVAVRGKDNKDVSS 123


>gb|PNY10518.1| DNA topoisomerase 1-like protein [Trifolium pratense]
          Length = 332

 Score = 82.4 bits (202), Expect = 3e-16
 Identities = 53/102 (51%), Positives = 63/102 (61%), Gaps = 2/102 (1%)
 Frame = +2

Query: 47  QLRAFQNPLNSLHLQPHTNLSIGIGNSVQRQNRFPFRCSFGGISISHVANAKSVNGFGFA 226
           QLR+  N L S  L    NL+I   N+VQ +NRF F CSFG  + + + +  SV+GFG  
Sbjct: 24  QLRSLHNLLISPQLPSLVNLTI---NAVQHRNRFSF-CSFGVTNFNTLKSVTSVSGFGLP 79

Query: 227 FHGNGIGRS--LLFSSRAVDSKRKKPVKTVAVRGKDDKDVSS 346
           F  NGIGRS   LF+     SKRKK VK VAVRGKD KD SS
Sbjct: 80  FRVNGIGRSSRFLFAVPEKKSKRKKSVKAVAVRGKDKKDASS 121


>dbj|GAU15218.1| hypothetical protein TSUD_09560 [Trifolium subterraneum]
          Length = 1068

 Score = 75.5 bits (184), Expect = 2e-13
 Identities = 53/104 (50%), Positives = 66/104 (63%), Gaps = 4/104 (3%)
 Frame = +2

Query: 47  QLRAFQNPLNSLHLQPHTNLSIGIGNSVQRQNRFPFRCSFG-GISISHVANAKSVNGFGF 223
           QLR+  NP  S HL    NL+    N+VQ +NRF FR SFG   + + + +  SV+GFG 
Sbjct: 25  QLRSLHNPSISPHLPSLVNLTT---NAVQHRNRFLFR-SFGVTTNFNTLKSVTSVSGFGS 80

Query: 224 AFHGN-GIGRSLLFSSRAVD--SKRKKPVKTVAVRGKDDKDVSS 346
               N G+GRSLLFSS A +  +KRKK VK VAV GKD KD+SS
Sbjct: 81  PSPANGGVGRSLLFSSVAPEKKNKRKKTVKAVAVSGKDKKDISS 124


>dbj|GAU15221.1| hypothetical protein TSUD_09580 [Trifolium subterraneum]
          Length = 681

 Score = 72.8 bits (177), Expect = 2e-12
 Identities = 53/104 (50%), Positives = 64/104 (61%), Gaps = 4/104 (3%)
 Frame = +2

Query: 47  QLRAFQNPLNSLHLQPHTNLSIGIGNSVQRQNRFPFRCSFG-GISISHVANAKSVNGFGF 223
           QLR+  NP  S HL    NL+    N+VQ +NRF FR SFG   + + + +  SV+GFG 
Sbjct: 25  QLRSLHNPPISPHLPSLVNLTT---NAVQHRNRFLFR-SFGVTTNFNSLKSVTSVSGFGL 80

Query: 224 AFHGN-GIGRSLLFSSRAVD--SKRKKPVKTVAVRGKDDKDVSS 346
            F  N G GRSL FSS A +  +KRKK VK VAV G D KDVSS
Sbjct: 81  PFTANGGDGRSLRFSSVAPEKKNKRKKTVKAVAVSGMDKKDVSS 124


>dbj|GAU15220.1| hypothetical protein TSUD_09590 [Trifolium subterraneum]
          Length = 1090

 Score = 72.8 bits (177), Expect = 2e-12
 Identities = 53/104 (50%), Positives = 64/104 (61%), Gaps = 4/104 (3%)
 Frame = +2

Query: 47  QLRAFQNPLNSLHLQPHTNLSIGIGNSVQRQNRFPFRCSFG-GISISHVANAKSVNGFGF 223
           QLR+  NP  S HL    NL+    N+VQ +NRF FR SFG   + + + +  SV+GFG 
Sbjct: 25  QLRSLHNPPISPHLPSLVNLTT---NAVQHRNRFLFR-SFGVTTNFNSLKSVTSVSGFGL 80

Query: 224 AFHGN-GIGRSLLFSSRAVD--SKRKKPVKTVAVRGKDDKDVSS 346
            F  N G GRSL FSS A +  +KRKK VK VAV G D KDVSS
Sbjct: 81  PFTANGGDGRSLRFSSVAPEKKNKRKKTVKAVAVSGMDKKDVSS 124


>ref|XP_013442265.1| DNA topoisomerase I, bacterial protein [Medicago truncatula]
 gb|KEH16290.1| DNA topoisomerase I, bacterial protein [Medicago truncatula]
          Length = 904

 Score = 72.4 bits (176), Expect = 2e-12
 Identities = 56/109 (51%), Positives = 68/109 (62%), Gaps = 9/109 (8%)
 Frame = +2

Query: 47  QLRAFQNPLNSL-HLQPHTNLSIGIGNSVQRQNRFPFRCSFGGIS-ISHVANAKSVN--- 211
           QLR+F NPL S   L PH N++     +V R+NRFP R SF  I+ +++  N KSVN   
Sbjct: 24  QLRSFHNPLISPPQLPPHVNVT---NFAVHRRNRFPIR-SFSVINNVTNFTNLKSVNVTS 79

Query: 212 --GFGFAFHGNGIGRSLLFSSRAVD--SKRKKPVKTVAVRGKDDKDVSS 346
             GFG  F  NG+GR   FSS A +  +KRKK VK VAVRGK DKDV S
Sbjct: 80  VNGFGSPF--NGVGRISPFSSTATEKKNKRKKTVKAVAVRGK-DKDVKS 125


>ref|XP_013465712.1| DNA topoisomerase I, bacterial protein [Medicago truncatula]
 gb|KEH39747.1| DNA topoisomerase I, bacterial protein [Medicago truncatula]
          Length = 1058

 Score = 72.4 bits (176), Expect = 2e-12
 Identities = 56/109 (51%), Positives = 68/109 (62%), Gaps = 9/109 (8%)
 Frame = +2

Query: 47  QLRAFQNPLNSL-HLQPHTNLSIGIGNSVQRQNRFPFRCSFGGIS-ISHVANAKSVN--- 211
           QLR+F NPL S   L PH N++     +V R+NRFP R SF  I+ +++  N KSVN   
Sbjct: 24  QLRSFHNPLISPPQLPPHVNVT---NFAVHRRNRFPIR-SFSVINNVTNFTNLKSVNVTS 79

Query: 212 --GFGFAFHGNGIGRSLLFSSRAVD--SKRKKPVKTVAVRGKDDKDVSS 346
             GFG  F  NG+GR   FSS A +  +KRKK VK VAVRGK DKDV S
Sbjct: 80  VNGFGSPF--NGVGRISPFSSTATEKKNKRKKTVKAVAVRGK-DKDVKS 125


>ref|XP_013442263.1| DNA topoisomerase I, bacterial protein [Medicago truncatula]
 gb|KEH16288.1| DNA topoisomerase I, bacterial protein [Medicago truncatula]
          Length = 1058

 Score = 72.4 bits (176), Expect = 2e-12
 Identities = 56/109 (51%), Positives = 68/109 (62%), Gaps = 9/109 (8%)
 Frame = +2

Query: 47  QLRAFQNPLNSL-HLQPHTNLSIGIGNSVQRQNRFPFRCSFGGIS-ISHVANAKSVN--- 211
           QLR+F NPL S   L PH N++     +V R+NRFP R SF  I+ +++  N KSVN   
Sbjct: 24  QLRSFHNPLISPPQLPPHVNVT---NFAVHRRNRFPIR-SFSVINNVTNFTNLKSVNVTS 79

Query: 212 --GFGFAFHGNGIGRSLLFSSRAVD--SKRKKPVKTVAVRGKDDKDVSS 346
             GFG  F  NG+GR   FSS A +  +KRKK VK VAVRGK DKDV S
Sbjct: 80  VNGFGSPF--NGVGRISPFSSTATEKKNKRKKTVKAVAVRGK-DKDVKS 125


>ref|XP_013465711.1| DNA topoisomerase I, bacterial protein [Medicago truncatula]
 gb|KEH39748.1| DNA topoisomerase I, bacterial protein [Medicago truncatula]
          Length = 1066

 Score = 72.4 bits (176), Expect = 2e-12
 Identities = 56/109 (51%), Positives = 68/109 (62%), Gaps = 9/109 (8%)
 Frame = +2

Query: 47  QLRAFQNPLNSL-HLQPHTNLSIGIGNSVQRQNRFPFRCSFGGIS-ISHVANAKSVN--- 211
           QLR+F NPL S   L PH N++     +V R+NRFP R SF  I+ +++  N KSVN   
Sbjct: 24  QLRSFHNPLISPPQLPPHVNVT---NFAVHRRNRFPIR-SFSVINNVTNFTNLKSVNVTS 79

Query: 212 --GFGFAFHGNGIGRSLLFSSRAVD--SKRKKPVKTVAVRGKDDKDVSS 346
             GFG  F  NG+GR   FSS A +  +KRKK VK VAVRGK DKDV S
Sbjct: 80  VNGFGSPF--NGVGRISPFSSTATEKKNKRKKTVKAVAVRGK-DKDVKS 125


>ref|XP_013442264.1| DNA topoisomerase I, bacterial protein [Medicago truncatula]
 gb|KEH16289.1| DNA topoisomerase I, bacterial protein [Medicago truncatula]
          Length = 1066

 Score = 72.4 bits (176), Expect = 2e-12
 Identities = 56/109 (51%), Positives = 68/109 (62%), Gaps = 9/109 (8%)
 Frame = +2

Query: 47  QLRAFQNPLNSL-HLQPHTNLSIGIGNSVQRQNRFPFRCSFGGIS-ISHVANAKSVN--- 211
           QLR+F NPL S   L PH N++     +V R+NRFP R SF  I+ +++  N KSVN   
Sbjct: 24  QLRSFHNPLISPPQLPPHVNVT---NFAVHRRNRFPIR-SFSVINNVTNFTNLKSVNVTS 79

Query: 212 --GFGFAFHGNGIGRSLLFSSRAVD--SKRKKPVKTVAVRGKDDKDVSS 346
             GFG  F  NG+GR   FSS A +  +KRKK VK VAVRGK DKDV S
Sbjct: 80  VNGFGSPF--NGVGRISPFSSTATEKKNKRKKTVKAVAVRGK-DKDVKS 125


>ref|XP_003588648.2| DNA topoisomerase I, bacterial protein [Medicago truncatula]
 gb|AES58899.2| DNA topoisomerase I, bacterial protein [Medicago truncatula]
          Length = 960

 Score = 68.2 bits (165), Expect = 7e-11
 Identities = 42/87 (48%), Positives = 55/87 (63%), Gaps = 1/87 (1%)
 Frame = +2

Query: 47  QLRAFQNPLNSLHLQPHTNLSIGIGNSVQRQNRFPFRCSFGGIS-ISHVANAKSVNGFGF 223
           QLR+F+NPL    + PH N++     ++QR NRFPFR SF  IS +++    K+VNGFGF
Sbjct: 26  QLRSFRNPL----ISPHVNVT---NFALQRCNRFPFR-SFSFISNVTNFTALKTVNGFGF 77

Query: 224 AFHGNGIGRSLLFSSRAVDSKRKKPVK 304
               NGIGRSL FSS   + K+ K  K
Sbjct: 78  RISANGIGRSLPFSSATPEKKKNKQKK 104