BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00019051
         (2121 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|GAU21788.1| hypothetical protein TSUD_329120, partial [Trifo...   296   e-134
gb|PNY15110.1| cysteine desulfurase mitochondrial-like [Trifoliu...   362   e-115
gb|PNX92709.1| cysteine desulfurase mitochondrial-like [Trifoliu...   299   7e-91
dbj|GAU41525.1| hypothetical protein TSUD_140560 [Trifolium subt...   315   7e-89
gb|PNY16581.1| cysteine desulfurase mitochondrial-like [Trifoliu...   291   5e-88
gb|PNY15174.1| ribonuclease H, partial [Trifolium pratense]           293   9e-82
dbj|GAU30026.1| hypothetical protein TSUD_161120 [Trifolium subt...   277   7e-76
dbj|GAU39028.1| hypothetical protein TSUD_59840 [Trifolium subte...   276   2e-75
gb|PNX69407.1| cysteine desulfurase mitochondrial-like, partial ...   201   9e-71
gb|PNX92710.1| ribonuclease H [Trifolium pratense]                    253   2e-68
gb|PNY15111.1| ribonuclease H [Trifolium pratense]                    252   1e-67
gb|PNY16582.1| hypothetical protein L195_g013306 [Trifolium prat...   227   4e-66
dbj|GAU43133.1| hypothetical protein TSUD_348540 [Trifolium subt...   229   1e-62
dbj|GAU49944.1| hypothetical protein TSUD_408390 [Trifolium subt...   216   4e-58
gb|PNX71169.1| cysteine desulfurase mitochondrial-like [Trifoliu...   211   9e-57
ref|XP_021716609.1| uncharacterized protein LOC110684459 [Chenop...   195   4e-53
emb|CCA66036.1| hypothetical protein [Beta vulgaris subsp. vulga...   209   5e-53
dbj|GAU33808.1| hypothetical protein TSUD_221460 [Trifolium subt...   206   5e-53
ref|XP_018857910.1| PREDICTED: uncharacterized protein LOC109019...   188   1e-50
ref|XP_021726600.1| uncharacterized protein LOC110693734 [Chenop...   188   1e-50

>dbj|GAU21788.1| hypothetical protein TSUD_329120, partial [Trifolium subterraneum]
          Length = 1086

 Score =  296 bits (759), Expect(2) = e-134
 Identities = 169/415 (40%), Positives = 255/415 (61%), Gaps = 15/415 (3%)
 Frame = -2

Query: 1934 EEQGLTVEDDDIA-EEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRLKNSVEVQDLSKN 1758
            E++ +TVE++++  ++E F RTL GK+WT+SPYN RAFKQT+TQAWR +N +E+QDL+KN
Sbjct: 13   EDKAITVEEEEVCNDDESFSRTLVGKIWTESPYNIRAFKQTMTQAWRSRNPIEIQDLNKN 72

Query: 1757 LFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSFWVRVYELPLKM 1578
            LFLF+F+++R+ + V KNGPW+F+RN+LIL R+SG+EQP+ + M  VSFWVRVY+LPLK+
Sbjct: 73   LFLFKFATRREADLVCKNGPWSFDRNLLILNRISGNEQPSELAMDSVSFWVRVYDLPLKL 132

Query: 1577 RSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNLCSEPR*FDTKGEAFESTS 1398
            RSE +A KLGN +G FEE D +E +R+G     KFLR+   +  +          F+   
Sbjct: 133  RSEGIAKKLGNTVGTFEEMDMKESNRMG-----KFLRMRVYVDLKKPLKRGSKLNFQGKE 187

Query: 1397 STKDFLLSAL-----LVGVLGIK*RTVRRLKIMMQKGTIEDIDEKEMTFGSWFRASPLPK 1233
               D+    L       G +G + R    ++    +    +++EK+  FG W RASPLPK
Sbjct: 188  IWVDYKYERLPNFCFACGRIGHQMRDCEDIE-DHDEVQYSELEEKQQAFGPWLRASPLPK 246

Query: 1232 ITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQ------SSEPPQVSKVPV 1071
            +++E +K+S+S TCSK+LF   S ++   S + K+ +  VEQ           + SK  +
Sbjct: 247  MSYEVKKESSSSTCSKSLFPSNSNNRGQNSGTAKEMNEEVEQQRASDDQHSHQKTSKATM 306

Query: 1070 KEPC---LRELFTSSKDIERVVESLGTVALSPTPKVGISNQSTEAKKGKKWTRNKVPRKP 900
             +       +     K++E VVESLG V +S   K+G   Q  +  KG+KW R +V  KP
Sbjct: 307  SKDADGDTPKQQNVQKEVEVVVESLGAVVISTQTKIGERIQHVKEGKGRKWVRQRV-AKP 365

Query: 899  RVSKGKEKVVELGKRLVLDVGTSDGDPEDKRGGDKKSKQDVVMADISENSDRVVL 735
            +  K  + + + GK  ++DV  +DG  E  RGG+KK + DVVM D + ++ R +L
Sbjct: 366  KPQKNAKTMGKTGKCSLVDVVVTDGTLEVLRGGEKKVRGDVVMKDCAASTVRALL 420



 Score =  212 bits (539), Expect(2) = e-134
 Identities = 108/223 (48%), Positives = 138/223 (61%)
 Frame = -1

Query: 669 SAVRALLRLTRSENPQVVFLIETKLKSFEMEVIRRKSGFDACLSMDYVGNGRERAGGIAL 490
           S VRALLRL R ENPQ+VFL+ET+LK+ E E+IR K GF  CL +D  G GRER GG+AL
Sbjct: 414 STVRALLRLNRVENPQIVFLMETRLKATEFEIIRSKLGFKNCLVVDCNGFGRERVGGLAL 473

Query: 489 LWKESMNISVISYSLNHIQCSIVDDEDGGNWFFIGVYGFPCEQDKWKTWDLIHSIGGSVS 310
           +W E +++++ SYSLNHI     D+E GG+W   G+YG+P E +K KTW LI S+     
Sbjct: 474 IWMEQLSVNISSYSLNHIHGRCDDEESGGSWGLTGIYGYPEEHNKRKTWQLIRSLASQNQ 533

Query: 309 SAWICVGDLNDTIAASEKQGGNPRSQHQLGIGRQSIDELNLLDLGFEGYPFTWPNGREER 130
             W+C GD ND + + EKQGGN RSQ QL  GRQ++ + + +DL F              
Sbjct: 534 GWWLCCGDFNDILDSHEKQGGNIRSQSQLSFGRQAVADCSSIDLEF-------------- 579

Query: 129 DNIQARLDRALGNASAINRFSPIKVSHLPHFKSDHAVVMVQLE 1
                           INRFSPIKV+HLP F SDHA V++ LE
Sbjct: 580 ----------------INRFSPIKVNHLPRFGSDHAAVVISLE 606


>gb|PNY15110.1| cysteine desulfurase mitochondrial-like [Trifolium pratense]
          Length = 425

 Score =  362 bits (928), Expect = e-115
 Identities = 206/431 (47%), Positives = 276/431 (64%), Gaps = 15/431 (3%)
 Frame = -2

Query: 1970 MEPWKDVPLSGAEEQGLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRLK 1791
            M+ WK+V LS  EE+G+  E++D   E++F RTLAGKLWTDSP+N RAFKQT+ QAWRLK
Sbjct: 1    MDSWKNVNLSKEEEEGVVWEEEDTYGEDLFRRTLAGKLWTDSPFNVRAFKQTMIQAWRLK 60

Query: 1790 NSVEVQDLSKNLFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSF 1611
            N VEV+DL KNLFLFRF++K+D + VLKNGPW+F+RN+LIL RVSG+EQPA++ M++V+F
Sbjct: 61   NQVEVEDLKKNLFLFRFATKKDADLVLKNGPWSFDRNLLILNRVSGEEQPADLEMNKVAF 120

Query: 1610 WVRVYELPLKMRSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNLCSEPR*F 1431
            WVRVYELPLK+RS+SMA KLGN++G FEE DP E +R G     +FLR+   +  + R  
Sbjct: 121  WVRVYELPLKVRSDSMAKKLGNIIGQFEETDPLERNRTG-----RFLRL--KVLIDLRKP 173

Query: 1430 DTKGEAFE-STSSTKDFLLSALLVGVLGIK*RTVRRLKIMMQKGTIEDI------DEKEM 1272
              +G   +    + K F     L     +  R   +LK   + G +E+       +EKE 
Sbjct: 174  LKRGTVIKYQDRNLKVFFKYERLPTFCFVCGRIGHQLKECEEAGELEEAGYEDLEEEKEH 233

Query: 1271 TFGSWFRASPLPKITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQSSEPP 1092
             FG W RASPLPK +FE RK+S+SG CSK+LF  TSTSK + S   K  ++ VEQ  +  
Sbjct: 234  GFGPWLRASPLPKPSFEPRKESSSGACSKSLFDSTSTSKNEGSGKEKSTEIEVEQQKKLA 293

Query: 1091 Q--VSKVPVKEPCLRELFTSSKDIERVVESLGTVALSPTPKVGISNQSTEAKKGKKWTRN 918
            Q   SK  V    L     +SK++E V ESLG V LS    +G  + S   K G+KWTR 
Sbjct: 294  QDEPSKTVV---ILNAKEITSKEVESVAESLGIVDLSKNVDIGQQDTSKGQKSGRKWTRK 350

Query: 917  KVPRKPRVSKGKEKVVELGKRLVLDVGTSDGDPEDKRGGDKKSKQDVVMAD------ISE 756
            K     +  K  +K +ELGKR +++V  S+G+PED  GG KK +QD+VM D      ++ 
Sbjct: 351  KAGPSKKGKKTTKKEIELGKRQLVEVMVSEGNPEDLVGGGKKRRQDIVMEDVNATVTVTA 410

Query: 755  NSDRVVLDDQH 723
                VVL++QH
Sbjct: 411  TEPEVVLENQH 421


>gb|PNX92709.1| cysteine desulfurase mitochondrial-like [Trifolium pratense]
          Length = 426

 Score =  299 bits (766), Expect = 7e-91
 Identities = 176/421 (41%), Positives = 260/421 (61%), Gaps = 17/421 (4%)
 Frame = -2

Query: 1934 EEQGLTVEDDDIA-EEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRLKNSVEVQDLSKN 1758
            E++ + VE D++  ++E F RTL GK+WT++PYN RAFKQT+TQAWR +N V++QDL+KN
Sbjct: 12   EDEAILVEADEMCNDDESFSRTLVGKIWTENPYNIRAFKQTMTQAWRCRNPVDIQDLNKN 71

Query: 1757 LFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSFWVRVYELPLKM 1578
            LFLF+F++K++ E V K GPW+F+RN+LIL R+SG+EQP+ + M RVSFWVRVY+LPLK+
Sbjct: 72   LFLFKFATKKEAELVWKTGPWSFDRNLLILNRISGNEQPSELEMDRVSFWVRVYDLPLKL 131

Query: 1577 RSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNLCSEPR*FDTKGEAFESTS 1398
            RSE MA KLGN+LG FEE D ++ +R+G     KFLRI  ++            +F+   
Sbjct: 132  RSEGMAKKLGNILGTFEEMDMKDCNRMG-----KFLRIRVSMDLRKPLKRGSKLSFQGKD 186

Query: 1397 STKDFLLSAL-----LVGVLGIK*RTVRRLKIMMQKGTIEDIDEKEMTFGSWFRASPLPK 1233
               D+    L       G +G + R    +          +++EK+  FG W RASPLPK
Sbjct: 187  IWVDYKYERLPNFCFACGRIGHQMRDCEEVD-DHDTDAYSELEEKDQAFGPWLRASPLPK 245

Query: 1232 ITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQSSE------PPQVSKV-- 1077
            + +E +K+++SGTCSK+LF+ TS SK   S + K ++  V+Q +       PP+ ++   
Sbjct: 246  VFYEVKKETSSGTCSKSLFTSTSNSKGQSSGTAKANEEEVDQQNATSGTEIPPKTNEKNH 305

Query: 1076 -PVKEPCLRELFTSSKDIERVVESLGTVALSPTPKVGISNQSTEAKKGKKWTRNKV--PR 906
                E  + ++ +  KD+E V ESLG V +S  P      QS++AK  KKW R+KV  PR
Sbjct: 306  NEAMEGVVEKVGSVHKDVEGVAESLGAVTISIVPISVEDKQSSKAK--KKWVRHKVSKPR 363

Query: 905  KPRVSKGKEKVVELGKRLVLDVGTSDGDPEDKRGGDKKSKQDVVMADISENSDRVVLDDQ 726
              + +K  EK  + GKR +++   ++    D    +KK K DV + D + +S  VVL+DQ
Sbjct: 364  NKQPAKLAEK--KGGKRSLVEARATEDTLADMLRREKKKKGDVEVEDYTVSSRLVVLEDQ 421

Query: 725  H 723
            H
Sbjct: 422  H 422


>dbj|GAU41525.1| hypothetical protein TSUD_140560 [Trifolium subterraneum]
          Length = 1610

 Score =  315 bits (807), Expect = 7e-89
 Identities = 174/376 (46%), Positives = 246/376 (65%), Gaps = 7/376 (1%)
 Frame = -2

Query: 1934 EEQGLTVEDDDIAEEE-VFHRTLAGKLWTDSPYNTRAFKQTITQAWRLKNSVEVQDLSKN 1758
            EE  +TVE ++I EEE  F RTL GKLWT++PYN RAFKQTI QAWRLKNS+EVQDL +N
Sbjct: 14   EEDCITVEAEEICEEEETFKRTLVGKLWTENPYNVRAFKQTIAQAWRLKNSIEVQDLEEN 73

Query: 1757 LFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSFWVRVYELPLKM 1578
            LFLFRF++K+D ++VL+NGPW+F+RN+LIL RVSG+EQP++++MH V+FWVRVY+LP K+
Sbjct: 74   LFLFRFTTKKDADTVLRNGPWSFDRNLLILHRVSGEEQPSDLDMHNVNFWVRVYDLPFKL 133

Query: 1577 RSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNL-CSEPR*FDTKGEAFEST 1401
            RSE+MA KLGN++GNFEE DP++ +R G     +FLR+  +L   +P    TK + F+  
Sbjct: 134  RSEAMAKKLGNIMGNFEEVDPKDANRTG-----RFLRLKASLDLRKPLKRGTKIK-FQDK 187

Query: 1400 SSTKDFLLSAL-----LVGVLGIK*RTVRRLKIMMQKGTIEDIDEKEMTFGSWFRASPLP 1236
            +   DF    L       G +G + +    ++  + +    DI EK   +G W RASPLP
Sbjct: 188  NMWVDFKYERLPNFCFACGKIGHQMKECEDVE-DVDENNYSDIAEKSQAYGPWLRASPLP 246

Query: 1235 KITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQSSEPPQVSKVPVKEPCL 1056
            +I  E RKD++SG+CSK LF  +S SK   S  +KD +  V+Q         V +++  +
Sbjct: 247  RIFEEPRKDASSGSCSKNLFPSSSQSKGGQSEGKKDKEQEVDQQ------PVVGIEKQLV 300

Query: 1055 RELFTSSKDIERVVESLGTVALSPTPKVGISNQSTEAKKGKKWTRNKVPRKPRVSKGKEK 876
                T++ D+E V ESLG VA+S +  VG + +S    KG+KW RNK  +  +    ++ 
Sbjct: 301  PTKATNNLDVEDVAESLGAVAISTSFVVGKTTKSQGGSKGRKWVRNKSSKPVKAQTARKL 360

Query: 875  VVELGKRLVLDVGTSD 828
              ELGKR ++DV  S+
Sbjct: 361  AKELGKRNLVDVAISE 376



 Score =  185 bits (469), Expect = 6e-45
 Identities = 106/223 (47%), Positives = 130/223 (58%)
 Frame = -1

Query: 669 SAVRALLRLTRSENPQVVFLIETKLKSFEMEVIRRKSGFDACLSMDYVGNGRERAGGIAL 490
           S VRAL RL R ENPQVVFL+ET+LK  E++ ++ K GF + L++D  G GRERAGG+AL
Sbjct: 375 SEVRALSRLIRLENPQVVFLMETRLKVPEIDRLKFKLGFSSGLAIDCKGVGRERAGGLAL 434

Query: 489 LWKESMNISVISYSLNHIQCSIVDDEDGGNWFFIGVYGFPCEQDKWKTWDLIHSIGGSVS 310
            WK+ M+I++ SYSLNHI    VD E    W   G+Y                       
Sbjct: 435 FWKDHMDITIKSYSLNHIHGQCVDVETNEPWDLTGIY----------------------- 471

Query: 309 SAWICVGDLNDTIAASEKQGGNPRSQHQLGIGRQSIDELNLLDLGFEGYPFTWPNGREER 130
                         A EK+GG  R+Q QL IGRQ+I E  L DLGFEGYP TW NGR   
Sbjct: 472 --------------AEEKKGGLVRTQGQLEIGRQAILESRLNDLGFEGYPLTWSNGRNSD 517

Query: 129 DNIQARLDRALGNASAINRFSPIKVSHLPHFKSDHAVVMVQLE 1
           DN Q R DRA+ +   INRFSPI V HLP + SDH V+++ LE
Sbjct: 518 DNKQCRQDRAMSSDEFINRFSPIHVLHLPRYGSDHVVLVITLE 560


>gb|PNY16581.1| cysteine desulfurase mitochondrial-like [Trifolium pratense]
          Length = 418

 Score =  291 bits (746), Expect = 5e-88
 Identities = 175/423 (41%), Positives = 251/423 (59%), Gaps = 7/423 (1%)
 Frame = -2

Query: 1970 MEPWKDVPLSGAEEQ-GLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRL 1794
            ME WK+V L+  EE+ G   +  ++ +EE+F  +L GKLWT +P+N R FKQ I QAWRL
Sbjct: 1    MENWKNVALTKEEEEDGFEADGVEVCDEEIFANSLVGKLWTTNPFNGRIFKQVIVQAWRL 60

Query: 1793 KNSVEVQDLSKNLFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVS 1614
            KN VE+QDL+KNLFLFRFSSKRDLE+VL+NGPW+F+RNI+IL+R+SGDEQP++I MH   
Sbjct: 61   KNPVEIQDLNKNLFLFRFSSKRDLETVLRNGPWSFDRNIVILKRISGDEQPSDIEMHSGE 120

Query: 1613 FWVRVYELPLKMRSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNLCSEPR* 1434
            FW R+Y+LPLK+RS+ MA KLG++LG F E D +E +R+G F   K      N       
Sbjct: 121  FWTRIYDLPLKLRSDEMARKLGDVLGKFVEVDNKESNRMGKFLWIKSTIDLRNPLKRGTI 180

Query: 1433 FDTKGEAFESTSSTKDFLLSALLVGVLGIK*RTVRRLKIMMQKGTIEDIDEKEMTFGSWF 1254
               +G++       +       + G +G + +    ++    +   EDI EKE+ FG W 
Sbjct: 181  IKFQGKSLRIFFKYERLPTFCFVCGRIGHQLKDCEDME-GKDETEFEDIVEKELPFGQWL 239

Query: 1253 RASPLPKITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQSSEPPQVSKVP 1074
            RASPLPKIT E +KD+++G+CSK LF+ TS SK    S  K+  + VEQ     + + + 
Sbjct: 240  RASPLPKITGEVKKDTSTGSCSKNLFTETSQSK--EGSVEKEKGLEVEQVMVASE-NILQ 296

Query: 1073 VKEPCLRELFTSSKDIERVVESLGTVALSPT-----PKVGISNQSTEAKKGKKWTRNKVP 909
            + +   +E   +++ +E V ESL  V +S +      K   +      K  KKWTR    
Sbjct: 297  IDKGATQEEGKNTQAVESVAESLSNVVISKSELGKFDKGSNTKPKPVPKPRKKWTRRAGT 356

Query: 908  RKPRVSKGKEKVVELGKRLVLDVGTSDGDPEDKRGGDKKSKQDVVMADIS-ENSDRVVLD 732
            +K + S     V ELGKR + +V   + +  +  GGD K +   +  D++ EN    VL+
Sbjct: 357  KKGKTS--DNVVAELGKRQLAEVTIVEEEAMELCGGDPKRR---LTTDVTCENLLEGVLE 411

Query: 731  DQH 723
            DQH
Sbjct: 412  DQH 414


>gb|PNY15174.1| ribonuclease H, partial [Trifolium pratense]
          Length = 1289

 Score =  293 bits (750), Expect = 9e-82
 Identities = 164/398 (41%), Positives = 244/398 (61%), Gaps = 13/398 (3%)
 Frame = -2

Query: 1970 MEPWKDVPLSGAEEQGLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRLK 1791
            M+ WK+ PL+  EE+ + V+D    E+E F R+L GKLWT+  +N R FKQ ++Q+WRLK
Sbjct: 88   MDKWKENPLTKEEEEEIVVDDTVACEDESFKRSLVGKLWTEDHFNVRIFKQVVSQSWRLK 147

Query: 1790 NSVEVQDLSKNLFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSF 1611
            N VE+QDLSKNLFLFRF+SKRDLE VL+NGPW+F+RN++IL+R+SG+EQP+++ M    F
Sbjct: 148  NPVEIQDLSKNLFLFRFTSKRDLEMVLRNGPWSFDRNLVILKRISGEEQPSDLEMFTAEF 207

Query: 1610 WVRVYELPLKMRSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNL-----CS 1446
            W R+Y+LPLK+RS++MA +LGN +G F EAD ++GHRLG     KFLR+  ++       
Sbjct: 208  WARIYDLPLKLRSDAMALRLGNTIGQFVEADSKDGHRLG-----KFLRVKVSIDLRKPLK 262

Query: 1445 EPR*FDTKGEAFESTSSTKDFLLSALLVGVLGIK*RTVRRLKIMMQKGTIEDIDEKEMTF 1266
                 + +G+        +       + G +G + +    ++   + G  EDI+EKE+ +
Sbjct: 263  RGTVLNYQGKRLHVFFKYERLPNFCYMCGRIGHQMKDCEEVEGNEEDG-YEDIEEKELPY 321

Query: 1265 GSWFRASPLPKITFEQRKDSNSGTCSKTLFSPTSTSKCDISS--SRKDDDVVVEQSSEPP 1092
            GSW +ASPLPKI+ + +K+ +S +CSK+LF  TSTSK        +  +  VV    +  
Sbjct: 322  GSWLKASPLPKISGDIKKEQSSSSCSKSLFGETSTSKVGSKEGLGKGLEIEVVSSLKKSN 381

Query: 1091 QVSKVPVKEPCLRELFTSSKDIERVVESLGTVALSPTPKVGI------SNQSTEAKKGKK 930
             V ++   E   R      K++E V ESLG VA+S T K+        + Q T  K  +K
Sbjct: 382  VVGEIDEIEETNRG--KDQKEVECVAESLGNVAIS-TQKMATFDIGSHTKQKTPVKAPRK 438

Query: 929  WTRNKVPRKPRVSKGKEKVVELGKRLVLDVGTSDGDPE 816
            W+R K  RK +     + + +LGKR +++V  S+G PE
Sbjct: 439  WSRKKGARKAKEPDQTQLIADLGKRQLVEVVISEGHPE 476



 Score =  271 bits (693), Expect = 4e-74
 Identities = 129/229 (56%), Positives = 167/229 (72%), Gaps = 3/229 (1%)
 Frame = -1

Query: 678  GNPSA---VRALLRLTRSENPQVVFLIETKLKSFEMEVIRRKSGFDACLSMDYVGNGRER 508
            G+P A   VRAL RL + ENP +VFL+ET+LK  EME I+ K GF +CLS+   G+GR+R
Sbjct: 473  GHPEAILGVRALSRLIKLENPHLVFLMETRLKVDEMERIKIKCGFSSCLSVACTGSGRDR 532

Query: 507  AGGIALLWKESMNISVISYSLNHIQCSIVDDEDGGNWFFIGVYGFPCEQDKWKTWDLIHS 328
            AGGI+LLW++ +N+SVI++SLNHI CSIVD E G NWF   +YGFP E +K KTWDLI  
Sbjct: 533  AGGISLLWQDQVNLSVINFSLNHILCSIVDGERGDNWFMSCMYGFPDEHNKRKTWDLIQQ 592

Query: 327  IGGSVSSAWICVGDLNDTIAASEKQGGNPRSQHQLGIGRQSIDELNLLDLGFEGYPFTWP 148
            I   V + WIC+GDLND ++  +K GGN RSQ Q+GIGRQ ++   L D+GF+GYP+TW 
Sbjct: 593  ISNQVGNKWICLGDLNDILSPDDKLGGNTRSQTQMGIGRQVMEACGLQDMGFDGYPYTWT 652

Query: 147  NGREERDNIQARLDRALGNASAINRFSPIKVSHLPHFKSDHAVVMVQLE 1
            NGR++ +NIQ RLDRA+     +NRFSP KV HL    SDHA +++ LE
Sbjct: 653  NGRQDSENIQCRLDRAMATEDFLNRFSPFKVVHLQRHGSDHAAILISLE 701


>dbj|GAU30026.1| hypothetical protein TSUD_161120 [Trifolium subterraneum]
          Length = 1957

 Score =  277 bits (709), Expect = 7e-76
 Identities = 154/378 (40%), Positives = 226/378 (59%)
 Frame = -2

Query: 1970 MEPWKDVPLSGAEEQGLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRLK 1791
            M  WKD+ +   E  G+T ED++  EEEV+ R+L GKLWT+S YN RAFKQTI   WRLK
Sbjct: 1    MARWKDIEIPADEIIGVTEEDEEQVEEEVYGRSLVGKLWTESAYNVRAFKQTIVDFWRLK 60

Query: 1790 NSVEVQDLSKNLFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSF 1611
            N VE Q+L KNL+LFRFSSKRDLE+VLKNGPW+F RNIL+L+R++G EQP+ + ++ +SF
Sbjct: 61   NPVETQELGKNLYLFRFSSKRDLENVLKNGPWSFGRNILVLKRINGTEQPSEMELNSMSF 120

Query: 1610 WVRVYELPLKMRSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNLCSEPR*F 1431
            W R+Y+LPLK+RSESMA KL +++G FEEAD ++ HR+G F  +K               
Sbjct: 121  WARIYDLPLKLRSESMAKKLSDVMGKFEEADTKDCHRMGRFLRAKVTIDLTKPLKRGTIV 180

Query: 1430 DTKGEAFESTSSTKDFLLSALLVGVLGIK*RTVRRLKIMMQKGTIEDIDEKEMTFGSWFR 1251
               G+  +     +       L G +G + R    +     +G  E+I+EKE+ FG W R
Sbjct: 181  RYNGKDLKVFFKYERLPTLCFLCGRIGHQLRDCDEIGGNEGEG-FEEIEEKELPFGPWLR 239

Query: 1250 ASPLPKITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQSSEPPQVSKVPV 1071
            ASPLP+ TF  ++DS S +CSK+LF+ TS+SK ++      ++V VE+ +E   + K  +
Sbjct: 240  ASPLPRNTFVTKRDSGSSSCSKSLFTGTSSSKAELIGDH-TEEVEVEKPNEATAI-KGHI 297

Query: 1070 KEPCLRELFTSSKDIERVVESLGTVALSPTPKVGISNQSTEAKKGKKWTRNKVPRKPRVS 891
                L    T++ +IE +  +L +  L+P     ++          KW+R K  +     
Sbjct: 298  ATKSLPIPTTTTHEIEEMAATLKSCDLTPKNTKPLTKGKANCSSKAKWSRVKGRKLKPTK 357

Query: 890  KGKEKVVELGKRLVLDVG 837
            +     + +GKR ++D G
Sbjct: 358  EPTSDTLIIGKRQLVDRG 375



 Score =  189 bits (479), Expect = 3e-46
 Identities = 88/174 (50%), Positives = 119/174 (68%)
 Frame = -1

Query: 522 NGRERAGGIALLWKESMNISVISYSLNHIQCSIVDDEDGGNWFFIGVYGFPCEQDKWKTW 343
           +GRERAGG+ + W +++N +  S+SLNHIQ  I D  +   W  IG+YGF  +  K  TW
Sbjct: 402 HGRERAGGLVIWWADNVNFNFTSFSLNHIQGDIKDQGEEETWTLIGMYGFSVDNRKRDTW 461

Query: 342 DLIHSIGGSVSSAWICVGDLNDTIAASEKQGGNPRSQHQLGIGRQSIDELNLLDLGFEGY 163
           +LI ++  +    W+CVGD ND ++ASEK+GGNPRS  QL +GR+ +D   L DLGF GY
Sbjct: 462 ELIKNLSFTACDRWMCVGDFNDIVSASEKKGGNPRSFTQLQVGREGMDFCGLNDLGFNGY 521

Query: 162 PFTWPNGREERDNIQARLDRALGNASAINRFSPIKVSHLPHFKSDHAVVMVQLE 1
           PFTW NGR   +NIQ RLDRAL  ++  +RF+PI V+HL  F+SDH+ + V LE
Sbjct: 522 PFTWSNGRIGAENIQCRLDRALATSAFNSRFTPINVNHLGRFRSDHSALAVCLE 575


>dbj|GAU39028.1| hypothetical protein TSUD_59840 [Trifolium subterraneum]
          Length = 1626

 Score =  276 bits (705), Expect = 2e-75
 Identities = 153/339 (45%), Positives = 216/339 (63%), Gaps = 5/339 (1%)
 Frame = -2

Query: 1970 MEPWKDVPLSGAEEQGLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRLK 1791
            MEPWK+ PLS  EE G+  ED +  E+  F ++LAGKLWT S YN RAFKQ+I + W+LK
Sbjct: 1    MEPWKNAPLSTEEEIGVIAEDLETEEDGYFTKSLAGKLWTTSSYNIRAFKQSIVEFWKLK 60

Query: 1790 NSVEVQDLSKNLFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSF 1611
            N VE+QDL KNL+LFRFS+KRDLE VLKNGPW+F+RNIL+L+R+SG EQP+ I+M  +SF
Sbjct: 61   NQVEIQDLGKNLYLFRFSTKRDLEHVLKNGPWSFDRNILVLKRISGIEQPSEIDMSSMSF 120

Query: 1610 WVRVYELPLKMRSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNLCSEPR*F 1431
            W RVY+LPLK+RSES+A KLG+++G FEE D +E HR+G F   K               
Sbjct: 121  WARVYDLPLKLRSESIAKKLGDLMGKFEEIDNKENHRMGCFLKIKVTIDLKKPMKRGTVV 180

Query: 1430 DTKGEAFESTSSTKDFLLSALLVGVLGIK*RTVRRLKIMMQKGTIEDIDEKEMTFGSWFR 1251
              +G+        +   +   + G +G + R     +    +G  E+I+EKE+ FG W R
Sbjct: 181  KFQGKDLRVFFKYERLPIFCFVCGRIGHQIRECEETEAQDGEG-FEEIEEKELPFGPWLR 239

Query: 1250 ASPLPKITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQSSEPPQVSKVPV 1071
            ASPLP+  +  +K+S S +CSK LFS +S+S+ D S   K  +V V+Q+    ++  + +
Sbjct: 240  ASPLPRANYVAKKESGSSSCSKNLFSGSSSSRAD-SVGDKMGEVEVDQAI--TKMKAIEI 296

Query: 1070 KEPCLRELFTSSK----DIERVVESLGTVALS-PTPKVG 969
            + P    +  S+     +IE+V ESLG+V +S  +PK G
Sbjct: 297  QLPNTNAVTNSTAKTFIEIEKVAESLGSVDISLASPKKG 335



 Score =  129 bits (324), Expect(2) = 7e-28
 Identities = 70/174 (40%), Positives = 96/174 (55%)
 Frame = -1

Query: 522 NGRERAGGIALLWKESMNISVISYSLNHIQCSIVDDEDGGNWFFIGVYGFPCEQDKWKTW 343
           +G++RAGGIAL W ++ NI++ SYSLNHI   I DD +G +W    +YGFP E +K KTW
Sbjct: 359 HGKDRAGGIALWWNDTTNITISSYSLNHINGKIDDDLNGESWSITRIYGFPEETNKRKTW 418

Query: 342 DLIHSIGGSVSSAWICVGDLNDTIAASEKQGGNPRSQHQLGIGRQSIDELNLLDLGFEGY 163
            LI+ I  S +  WIC GDLNDT++ASEK G   R              L+++ +G    
Sbjct: 419 ALINEISASTAENWICFGDLNDTLSASEKAGEKARR------------TLSVVLIGL--- 463

Query: 162 PFTWPNGREERDNIQARLDRALGNASAINRFSPIKVSHLPHFKSDHAVVMVQLE 1
                                + ++S  NRF PIKV+HLP F SDH+ + + LE
Sbjct: 464 --------------------CMASSSFTNRFGPIKVTHLPRFGSDHSAIQILLE 497



 Score = 25.8 bits (55), Expect(2) = 7e-28
 Identities = 10/14 (71%), Positives = 12/14 (85%)
 Frame = -2

Query: 665 QFEPC*GSQEVKIP 624
           QFEPC GS+ +KIP
Sbjct: 342 QFEPCQGSKTLKIP 355


>gb|PNX69407.1| cysteine desulfurase mitochondrial-like, partial [Trifolium pratense]
          Length = 234

 Score =  201 bits (512), Expect(2) = 9e-71
 Identities = 88/159 (55%), Positives = 123/159 (77%)
 Frame = -2

Query: 1970 MEPWKDVPLSGAEEQGLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRLK 1791
            ME WK + L   EE+G  +E +++ E++ F+R+L GK+WT + +N R FKQ I QAWRLK
Sbjct: 1    MEDWKKIDLFKDEEEGFAIEGNELVEDDTFNRSLVGKIWTTNSFNARIFKQVIVQAWRLK 60

Query: 1790 NSVEVQDLSKNLFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSF 1611
            N VEVQ+L+KNLFLF+F++KRD E VLKNGPW+F+RN++ILER+SG+EQP+ + M    F
Sbjct: 61   NPVEVQELNKNLFLFKFTTKRDAEGVLKNGPWSFDRNLVILERISGEEQPSKLEMFNGVF 120

Query: 1610 WVRVYELPLKMRSESMATKLGNMLGNFEEADPREGHRLG 1494
            W RVY+LPLK+R++ +A KLG+ +G F E D  EG+R+G
Sbjct: 121  WARVYDLPLKLRTDVVAVKLGDTMGKFVEVDNNEGNRMG 159



 Score = 97.1 bits (240), Expect(2) = 9e-71
 Identities = 42/67 (62%), Positives = 54/67 (80%)
 Frame = -1

Query: 1497 GRFLRLKVSPDLRKPLLRATVI*YQGRSI*IHFKYKRLPTFCFACGRIGHQVKDCEEIED 1318
            G+ LR+K + DLRKPL R TVI YQG+S+ + FKY+RLPTFC+ACG+IGHQ+KDCE +E 
Sbjct: 159  GKCLRIKTTVDLRKPLKRGTVITYQGKSLRVFFKYERLPTFCYACGKIGHQIKDCETLEG 218

Query: 1317 NDAEGYD 1297
            N   G+D
Sbjct: 219  NTDTGFD 225


>gb|PNX92710.1| ribonuclease H [Trifolium pratense]
          Length = 1052

 Score =  253 bits (647), Expect = 2e-68
 Identities = 121/203 (59%), Positives = 151/203 (74%)
 Frame = -1

Query: 609 IETKLKSFEMEVIRRKSGFDACLSMDYVGNGRERAGGIALLWKESMNISVISYSLNHIQC 430
           +ET+LK+FE++ IR K GF  CLS+D  G+GR+RAGGI+L+W E ++I++ SYSLNHI  
Sbjct: 1   METRLKAFEVDNIRNKLGFKNCLSVDCRGSGRDRAGGISLMWMEHLSITINSYSLNHIHG 60

Query: 429 SIVDDEDGGNWFFIGVYGFPCEQDKWKTWDLIHSIGGSVSSAWICVGDLNDTIAASEKQG 250
              D+E G  W   G+YGFP E +K KTW LI S+   V+  W+C GDLND + + EKQG
Sbjct: 61  FCDDEETGEAWSLTGIYGFPEEHNKKKTWQLISSLASQVAGNWLCCGDLNDILDSQEKQG 120

Query: 249 GNPRSQHQLGIGRQSIDELNLLDLGFEGYPFTWPNGREERDNIQARLDRALGNASAINRF 70
           GN RSQ QL +GR ++ E +L DLGFEGYPFTW NGREE +NIQ+RLDRALGN   INRF
Sbjct: 121 GNIRSQGQLALGRHTVAECHLNDLGFEGYPFTWTNGREEDENIQSRLDRALGNEGFINRF 180

Query: 69  SPIKVSHLPHFKSDHAVVMVQLE 1
           SPIKV HLP F SDHA V++ LE
Sbjct: 181 SPIKVCHLPRFGSDHAAVLICLE 203


>gb|PNY15111.1| ribonuclease H [Trifolium pratense]
          Length = 1334

 Score =  252 bits (644), Expect = 1e-67
 Identities = 115/203 (56%), Positives = 148/203 (72%)
 Frame = -1

Query: 609 IETKLKSFEMEVIRRKSGFDACLSMDYVGNGRERAGGIALLWKESMNISVISYSLNHIQC 430
           +ET+LK  EME I+R+ GF   +S+D  G+GRERAGGI+LLW + +++SVISYS NHI C
Sbjct: 1   METRLKEDEMEKIKRRCGFSFGISVDCRGSGRERAGGISLLWSDQVSLSVISYSFNHILC 60

Query: 429 SIVDDEDGGNWFFIGVYGFPCEQDKWKTWDLIHSIGGSVSSAWICVGDLNDTIAASEKQG 250
           S  D +DG NWF  G+YGFP E +KWKTW L++ +   V S W+C GDLND +++ EK G
Sbjct: 61  SCADGDDGANWFLSGIYGFPEEFNKWKTWQLVNQLSTQVGSRWVCFGDLNDVLSSEEKMG 120

Query: 249 GNPRSQHQLGIGRQSIDELNLLDLGFEGYPFTWPNGREERDNIQARLDRALGNASAINRF 70
           G  R+Q+QLG+GRQ + +  LLD GFEGYPFTW NGR+  +NIQ RLDR L      NRF
Sbjct: 121 GVARTQNQLGLGRQCMADCGLLDPGFEGYPFTWSNGRQNEENIQCRLDRTLVTIDFQNRF 180

Query: 69  SPIKVSHLPHFKSDHAVVMVQLE 1
           SPI+V HLP + SDHA +M+ LE
Sbjct: 181 SPIRVVHLPRYGSDHAALMILLE 203


>gb|PNY16582.1| hypothetical protein L195_g013306 [Trifolium pratense]
          Length = 228

 Score =  227 bits (579), Expect = 4e-66
 Identities = 105/195 (53%), Positives = 143/195 (73%)
 Frame = -1

Query: 585 EMEVIRRKSGFDACLSMDYVGNGRERAGGIALLWKESMNISVISYSLNHIQCSIVDDEDG 406
           E+E +R + GF+A +++   G+G++RAGGIAL W ++ NI++ SYSLNHI  ++ DD +G
Sbjct: 5   EIERVRIRCGFNAGIAVGCTGHGKDRAGGIALWWNDATNITISSYSLNHINGTVGDDLNG 64

Query: 405 GNWFFIGVYGFPCEQDKWKTWDLIHSIGGSVSSAWICVGDLNDTIAASEKQGGNPRSQHQ 226
             W   G+YGFP E +K KTW LI+ I  S +  WIC GDLND ++A+EK GGNPRS  Q
Sbjct: 65  EPWSITGIYGFPEEYNKRKTWALINEISTSAAENWICFGDLNDIVSATEKVGGNPRSLTQ 124

Query: 225 LGIGRQSIDELNLLDLGFEGYPFTWPNGREERDNIQARLDRALGNASAINRFSPIKVSHL 46
           L +GR +++E +L DLGF GYPFTW NGRE  +NIQ RLDRA+ ++   NRFSPIKV+HL
Sbjct: 125 LNLGRVTMEECSLTDLGFTGYPFTWTNGREGEENIQCRLDRAMASSGFSNRFSPIKVTHL 184

Query: 45  PHFKSDHAVVMVQLE 1
           P F+SDH+ + + LE
Sbjct: 185 PRFRSDHSALQILLE 199


>dbj|GAU43133.1| hypothetical protein TSUD_348540 [Trifolium subterraneum]
          Length = 608

 Score =  229 bits (585), Expect = 1e-62
 Identities = 128/321 (39%), Positives = 202/321 (62%), Gaps = 6/321 (1%)
 Frame = -2

Query: 1934 EEQGLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRLKNSVEVQDLSKNL 1755
            + +GL V++ ++ ++ +F RTLA KLWT++ YN +AFKQT  Q+W+L+N VEVQDL+KNL
Sbjct: 288  QNKGLIVDEVEVYKDGLFCRTLAVKLWTNTSYNAKAFKQTTIQSWKLRNPVEVQDLNKNL 347

Query: 1754 FLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSFWVRVYELPLKMR 1575
            +LFRFS+K+D E+VL+NGPW+F++N++IL++++G+EQPA  +MH+VSFWVR+Y+LPL++R
Sbjct: 348  YLFRFSTKKDAENVLQNGPWSFDKNLIILQKITGEEQPAEFDMHKVSFWVRIYDLPLQLR 407

Query: 1574 SESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNLCSEPR*FDTKGEAFESTSS 1395
            S+S+A K+G+ +G  EE   RE +R G+F   + L               +G +      
Sbjct: 408  SDSIAKKIGDFIGVHEETYQREIYRTGSFLRIRVLMDLKKPLMRGTVVCYQGRSLRVFFK 467

Query: 1394 TKDFLLSALLVGVLGIK*RTVRRLKIMMQKGTIEDIDEKEMTFGSWFRASPLPKITFEQR 1215
             +         G +G + +T    +   ++    +++EKE +FG W +ASP PK  +E+ 
Sbjct: 468  YERLPTFCFNCGRIGHQKKTCEYNE--EEEEGFGEVEEKEYSFGPWLKASPPPKTNYEE- 524

Query: 1214 KDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQSSEPPQVSKVPVKEPCL------R 1053
            KDSNS +C K+LF+ TS+S  + S   K  +V VEQ+           KE CL      +
Sbjct: 525  KDSNSSSCRKSLFACTSSSIVEESEPIKGKEVKVEQNK-----GNAKSKEQCLDFEESGK 579

Query: 1052 ELFTSSKDIERVVESLGTVAL 990
            +L  ++K++E V ES   V L
Sbjct: 580  KL--NNKEVENVAESFERVTL 598



 Score =  226 bits (576), Expect = 2e-61
 Identities = 120/295 (40%), Positives = 192/295 (65%), Gaps = 6/295 (2%)
 Frame = -2

Query: 1970 MEPWKDVPLSGAE-EQGLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRL 1794
            ME WK+   S  E ++ L +++ +  ++E+FHRTLA KLWT++ YN +AFKQT  Q+W+L
Sbjct: 1    MEQWKNFKFSNQELQKSLNIDEFESYKDELFHRTLAVKLWTNTTYNAKAFKQTTIQSWKL 60

Query: 1793 KNSVEVQDLSKNLFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVS 1614
            KN V+VQDL+KNL+LFRFS+K D ++V  NGPW F+++ ++L +++G+EQ  N +M ++ 
Sbjct: 61   KNPVQVQDLNKNLYLFRFSTKNDADNVFNNGPWCFDKSHIVLRKITGEEQLENFDMDKIL 120

Query: 1613 FWVRVYELPLKMRSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNL-CSEPR 1437
            FWVRVY+LPLK+RS+S+A K G+++G +E  D R+ +R+G     +FLRI   L  ++P 
Sbjct: 121  FWVRVYDLPLKLRSDSIAKKFGDVIGVYEGIDQRDVYRMG-----RFLRIRVLLDLNKPL 175

Query: 1436 *FDT----KGEAFESTSSTKDFLLSALLVGVLGIK*RTVRRLKIMMQKGTIEDIDEKEMT 1269
               T    +G         +       + G +G + +      +  +    E++ EKE +
Sbjct: 176  VRGTLVCYQGRNIRVFFKYEKLQRFCFICGRIGHQMKVCDE-NVDEENEGCEEVVEKEYS 234

Query: 1268 FGSWFRASPLPKITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQS 1104
            FG W RASP PKI  E++KD +S +C K+LF+ TS+S+ +   +RK ++VV+EQ+
Sbjct: 235  FGQWLRASPPPKINNEEKKDRSSSSCRKSLFAYTSSSEVEDLETRKGEEVVMEQN 289


>dbj|GAU49944.1| hypothetical protein TSUD_408390 [Trifolium subterraneum]
          Length = 560

 Score =  216 bits (550), Expect = 4e-58
 Identities = 116/280 (41%), Positives = 171/280 (61%), Gaps = 2/280 (0%)
 Frame = -2

Query: 1970 MEPWKDVPLSGAEEQGLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRLK 1791
            M+ WK V LS  EE+ +   +  +  +E F R+L GKLWT+  +N R FKQ I+Q W LK
Sbjct: 1    MQDWKKVSLSKEEEE-IVAGESVVCSDESFKRSLVGKLWTEDHFNVRIFKQVISQDWSLK 59

Query: 1790 NSVEVQDLSKNLFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSF 1611
            N +E+QDLSKNLFLFRF++KRDLE+VLKNG W+F+RN+++L+R+SG+EQP+++ +    F
Sbjct: 60   NQIEIQDLSKNLFLFRFATKRDLETVLKNGLWSFDRNLVVLKRISGEEQPSDLEIFSADF 119

Query: 1610 WVRVYELPLKMRSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNLCSEPR*F 1431
            W R+Y+LPLK+RS++MA KLGN +G F E D +EGHRLG     KFLR+           
Sbjct: 120  WARIYDLPLKLRSDAMAEKLGNTIGKFVEVDIKEGHRLG-----KFLRL----------- 163

Query: 1430 DTKGEAFESTSSTKDFLLSALLVGVLGIK*RTVRRLKIMMQKGTIEDIDEKEMTFGSWFR 1251
                   ++T   +  L    ++   G      ++L++  +K T             W  
Sbjct: 164  -------KTTVDLRKPLKRGTVINFQG------KKLQVFFKKRT-----------PIWLM 199

Query: 1250 AS--PLPKITFEQRKDSNSGTCSKTLFSPTSTSKCDISSS 1137
            A   PLPK++ + +K+ +  +C+K+LFS TSTSK +   S
Sbjct: 200  AESFPLPKVSSDIKKEQSPSSCNKSLFSETSTSKGESKES 239



 Score =  185 bits (469), Expect = 7e-47
 Identities = 89/195 (45%), Positives = 119/195 (61%)
 Frame = -1

Query: 585 EMEVIRRKSGFDACLSMDYVGNGRERAGGIALLWKESMNISVISYSLNHIQCSIVDDEDG 406
           EME IR + GF +C+ +   G+GR+RAGG++                    C    D  G
Sbjct: 278 EMERIRNRCGFSSCILVACSGSGRDRAGGLS--------------------CGRTRDR-G 316

Query: 405 GNWFFIGVYGFPCEQDKWKTWDLIHSIGGSVSSAWICVGDLNDTIAASEKQGGNPRSQHQ 226
            +WF   +YG P E +KW+TW+LI      V   W+C+GDLND +   +K+GG  RSQ Q
Sbjct: 317 DDWFLSCIYGHPEENNKWRTWELIKQTHDQVGHKWLCIGDLNDMLQVDDKKGGLVRSQSQ 376

Query: 225 LGIGRQSIDELNLLDLGFEGYPFTWPNGREERDNIQARLDRALGNASAINRFSPIKVSHL 46
           LGIG Q+++   L D+GFEGYPFTW NGR+   N+Q RLD+ALG    +NRFSP KVSHL
Sbjct: 377 LGIGTQTVEACGLNDMGFEGYPFTWTNGRQRSKNVQCRLDKALGTKDFLNRFSPWKVSHL 436

Query: 45  PHFKSDHAVVMVQLE 1
             + SDHA +++QLE
Sbjct: 437 QRYGSDHAAILIQLE 451


>gb|PNX71169.1| cysteine desulfurase mitochondrial-like [Trifolium pratense]
          Length = 521

 Score =  211 bits (538), Expect = 9e-57
 Identities = 121/289 (41%), Positives = 185/289 (64%), Gaps = 12/289 (4%)
 Frame = -2

Query: 1934 EEQGLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRLKNSVEVQDLSKNL 1755
            + +GL +++ +  ++ +F RTL  KLWT++ YN +AFKQT  Q+W+L+N VEVQDL+KNL
Sbjct: 241  QNKGLVIDEVEAYKDRLFCRTLTVKLWTNTSYNAKAFKQTTIQSWKLRNPVEVQDLNKNL 300

Query: 1754 FLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVSFWVRVYELPLKMR 1575
            +LFRFS+K+D E+VLKNGPW+F++N++IL++++G+EQ A  +MH+VSF VR+Y+LPL++R
Sbjct: 301  YLFRFSTKKDAENVLKNGPWSFDKNLIILQKITGEEQLAEFDMHKVSFLVRIYDLPLQLR 360

Query: 1574 SESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNLCSEPR*FDTKGE--AFEST 1401
            S+S+A  +G+ +G  EE D  + +R G+     FLRI   +  + +   T+G    ++  
Sbjct: 361  SDSIAKTIGDFIGVHEETDQLKINRTGS-----FLRI--RVLIDLKKPLTRGTVVCYQGR 413

Query: 1400 SSTKDFLLSALLVGVLGIK*RTVRRLKIMMQKGTIED----------IDEKEMTFGSWFR 1251
            S    F    L               +I  QK T ED          ++EKE +FG W +
Sbjct: 414  SLRVFFKYERLPTFCFNCG-------RIGHQKKTCEDNEEEEEGFGEVEEKEYSFGPWLK 466

Query: 1250 ASPLPKITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQS 1104
            AS  PKI +E+ KDSNS +CSK+LF+ TS+S  + S   K  +V VEQ+
Sbjct: 467  ASLPPKINYEE-KDSNSSSCSKSLFACTSSSIVEESEPIKGKEVKVEQN 514



 Score =  172 bits (435), Expect = 2e-42
 Identities = 74/150 (49%), Positives = 114/150 (76%), Gaps = 1/150 (0%)
 Frame = -2

Query: 1970 MEPWKDVPLSGAE-EQGLTVEDDDIAEEEVFHRTLAGKLWTDSPYNTRAFKQTITQAWRL 1794
            ME WK+   S  E ++ L +++ +  ++E+FH TLA KLWT++ YN +AFKQT  Q+WRL
Sbjct: 1    MEQWKNFTFSNQELQKSLNIDESESYKDELFHHTLAVKLWTNTNYNAKAFKQTTIQSWRL 60

Query: 1793 KNSVEVQDLSKNLFLFRFSSKRDLESVLKNGPWNFNRNILILERVSGDEQPANINMHRVS 1614
            KN  EVQDL+KNL+LFRFS+K D ++V  NGPW F++N ++L +++G+E P   +M ++ 
Sbjct: 61   KNPAEVQDLNKNLYLFRFSTKEDADNVYNNGPWCFDKNHVVLRKITGEEHPTEFDMDKIL 120

Query: 1613 FWVRVYELPLKMRSESMATKLGNMLGNFEE 1524
            FWVR+Y+LPLK+RS+S+A K+G+++G  +E
Sbjct: 121  FWVRIYDLPLKLRSDSIAKKIGDVIGVMKE 150



 Score = 65.1 bits (157), Expect(2) = 3e-08
 Identities = 28/63 (44%), Positives = 45/63 (71%)
 Frame = -2

Query: 1292 DIDEKEMTFGSWFRASPLPKITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVV 1113
            +++EKE +FG W RASP PK  +E++KD +S +C K LF+ TS+S+ +   +RK + V +
Sbjct: 180  EVEEKEYSFGEWLRASPPPKFNYEEKKDRSSSSCRKFLFAYTSSSEVEDLETRKGEGVEM 239

Query: 1112 EQS 1104
            EQ+
Sbjct: 240  EQN 242



 Score = 23.9 bits (50), Expect(2) = 3e-08
 Identities = 9/17 (52%), Positives = 12/17 (70%)
 Frame = -1

Query: 1353 GHQVKDCEEIEDNDAEG 1303
            GHQ+K C+E  D + EG
Sbjct: 161  GHQMKVCDENVDEENEG 177


>ref|XP_021716609.1| uncharacterized protein LOC110684459 [Chenopodium quinoa]
          Length = 295

 Score =  195 bits (495), Expect = 4e-53
 Identities = 104/234 (44%), Positives = 141/234 (60%), Gaps = 3/234 (1%)
 Frame = -1

Query: 702 LSWNCRGLGNPSAVRALLRLTRSENPQVVFLIETKLKSFEMEVIRRKSGFDACLSMDYVG 523
           LSWNCRGLG+  A+ AL R+   E P +VFL+ETKLK+FEME I+ K  F +C  +D  G
Sbjct: 5   LSWNCRGLGSTDAINALKRIVFLEIPHMVFLMETKLKAFEMEKIKYKIKFSSCFIVDCEG 64

Query: 522 NGRERAGGIALLWKESMNISVISYSLNHIQC---SIVDDEDGGNWFFIGVYGFPCEQDKW 352
            GR R GG+ALLW  S+++++ S+SLNHI     SI  DE    W F G+YG P E++K+
Sbjct: 65  EGRRRRGGLALLWNNSIDVNIRSFSLNHIDARVRSITQDE----WRFSGIYGHPDEENKY 120

Query: 351 KTWDLIHSIGGSVSSAWICVGDLNDTIAASEKQGGNPRSQHQLGIGRQSIDELNLLDLGF 172
           K   L+ S+ G   + W+C GDL   + + EKQGG      +  I R  +    L DLGF
Sbjct: 121 KIGQLLISLKGGTDTPWLCGGDLILMLHSGEKQGGRVFDMEEAEILRDVVGVCELEDLGF 180

Query: 171 EGYPFTWPNGREERDNIQARLDRALGNASAINRFSPIKVSHLPHFKSDHAVVMV 10
            G+ FTW N R   ++IQ RLDR L N +  + F    V+HL   KSDH  +++
Sbjct: 181 IGHDFTWSNNRGGEESIQERLDRFLANRTWRDAFQGSFVTHLTKRKSDHLPILL 234


>emb|CCA66036.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
          Length = 1369

 Score =  209 bits (532), Expect = 5e-53
 Identities = 105/234 (44%), Positives = 151/234 (64%)
 Frame = -1

Query: 702 LSWNCRGLGNPSAVRALLRLTRSENPQVVFLIETKLKSFEMEVIRRKSGFDACLSMDYVG 523
           LSWNCRG+G+PSA+ AL RL  SENPQ+VFL ETKLKS+EME +++K  ++  +++D  G
Sbjct: 5   LSWNCRGMGSPSALSALRRLLASENPQIVFLSETKLKSYEMESVKKKLKWEHMVAVDCEG 64

Query: 522 NGRERAGGIALLWKESMNISVISYSLNHIQCSIVDDEDGGNWFFIGVYGFPCEQDKWKTW 343
             R+R GG+A+LW+  + + V+S S NHI   +V +E  G W F G+YG+P E+ K KT 
Sbjct: 65  ECRKRRGGLAMLWRSEIKVQVMSMSSNHIDI-VVGEEAQGEWRFTGIYGYPEEEHKDKTG 123

Query: 342 DLIHSIGGSVSSAWICVGDLNDTIAASEKQGGNPRSQHQLGIGRQSIDELNLLDLGFEGY 163
            L+ ++  +    W+C GD N  + ASEK+GG+  +  +  I R +++E + +DLGF GY
Sbjct: 124 ALLSALARASRRPWLCGGDFNLMLVASEKKGGDGFNSREADIFRNAMEECHFMDLGFVGY 183

Query: 162 PFTWPNGREERDNIQARLDRALGNASAINRFSPIKVSHLPHFKSDHAVVMVQLE 1
            FTW N R    NIQ RLDR + N     +F    VSHLP  KSDH  ++  ++
Sbjct: 184 EFTWTNNRGGDANIQERLDRFVANDLWKIKFPGSFVSHLPKRKSDHVPIVASVK 237


>dbj|GAU33808.1| hypothetical protein TSUD_221460 [Trifolium subterraneum]
          Length = 809

 Score =  206 bits (525), Expect = 5e-53
 Identities = 109/222 (49%), Positives = 137/222 (61%)
 Frame = -1

Query: 669 SAVRALLRLTRSENPQVVFLIETKLKSFEMEVIRRKSGFDACLSMDYVGNGRERAGGIAL 490
           S VRALLRL R ENP  VFL+ET+LKSFEME ++++ GF++CL++D  G G+ERAG    
Sbjct: 259 SEVRALLRLIRLENPMCVFLMETRLKSFEMEGLKQRCGFNSCLTVDCRGLGKERAG---- 314

Query: 489 LWKESMNISVISYSLNHIQCSIVDDEDGGNWFFIGVYGFPCEQDKWKTWDLIHSIGGSVS 310
                                  D+ED  N  F  +YG+P E +K KTW LI  +  +V 
Sbjct: 315 -----------------------DEEDNQNCHFTCMYGYPEECNKKKTWQLITELAATVE 351

Query: 309 SAWICVGDLNDTIAASEKQGGNPRSQHQLGIGRQSIDELNLLDLGFEGYPFTWPNGREER 130
           S WIC GDLND   + EK GGN RS +QL +GR +ID   L+DLG+EG+PFTW NGRE  
Sbjct: 352 SKWICFGDLNDIFTSDEKLGGNTRSPNQLMLGRNAIDTCRLIDLGYEGHPFTWTNGRELE 411

Query: 129 DNIQARLDRALGNASAINRFSPIKVSHLPHFKSDHAVVMVQL 4
           +NIQ RLDRAL     INRFSPI+V HLP       ++ V L
Sbjct: 412 ENIQCRLDRALATEEFINRFSPIRVLHLPIVSEAEKILQVLL 453



 Score = 95.5 bits (236), Expect = 2e-16
 Identities = 41/67 (61%), Positives = 54/67 (80%)
 Frame = -1

Query: 1497 GRFLRLKVSPDLRKPLLRATVI*YQGRSI*IHFKYKRLPTFCFACGRIGHQVKDCEEIED 1318
            G+FLR+KV+ DLRKPL R  V+ YQG+++ + FKY+RLPTFCFACG+IGHQ+KDCEE+E 
Sbjct: 79   GKFLRVKVTVDLRKPLKRGIVVKYQGKNLRVFFKYERLPTFCFACGKIGHQIKDCEEMEG 138

Query: 1317 NDAEGYD 1297
                 +D
Sbjct: 139  KGETEFD 145



 Score = 82.0 bits (201), Expect = 3e-12
 Identities = 72/267 (26%), Positives = 112/267 (41%), Gaps = 17/267 (6%)
 Frame = -2

Query: 1577 RSESMATKLGNMLGNFEEADPREGHRLGAF*GSKFLRI*GNLCSEPR*FDTKG------- 1419
            RS++MA KLG+++G F E D ++G+RLG     KFLR+   +  + R    +G       
Sbjct: 52   RSDAMAKKLGDVIGEFVEVDLKDGNRLG-----KFLRV--KVTVDLRKPLKRGIVVKYQG 104

Query: 1418 ---EAFESTSSTKDFLLSALLVGVLGIK*RTVRRLKIMMQKGTIE--DIDEKEMTFGSWF 1254
                 F        F  +   +G        ++  + M  KG  E  +I+EKE  FG W 
Sbjct: 105  KNLRVFFKYERLPTFCFACGKIG------HQIKDCEEMEGKGETEFDEIEEKEFPFGQWM 158

Query: 1253 RASPLPKITFEQRKDSNSGTCSKTLFSPTSTSKCDISSSRKDDDVVVEQSSEPPQVSKVP 1074
            +ASPLPK   + +KD++S +C K+LF                                  
Sbjct: 159  KASPLPKALLDIKKDNSSSSCGKSLF---------------------------------- 184

Query: 1073 VKEPCLRELFTSSKDIERVVESLG-----TVALSPTPKVGISNQSTEAKKGKKWTRNKVP 909
                       +  ++E V ESLG     T  L    K   +    + K+ +KW R K  
Sbjct: 185  -----------AESEVESVAESLGNAEITTQVLPQVDKGSHTKPKPQKKQARKWARKKGA 233

Query: 908  RKPRVSKGKEKVVELGKRLVLDVGTSD 828
            RK    + ++ V ELGKR +++V  S+
Sbjct: 234  RKEASQEQQQLVTELGKRQLVEVTISE 260


>ref|XP_018857910.1| PREDICTED: uncharacterized protein LOC109019980 [Juglans regia]
          Length = 305

 Score =  188 bits (478), Expect = 1e-50
 Identities = 102/238 (42%), Positives = 138/238 (57%), Gaps = 1/238 (0%)
 Frame = -1

Query: 711 MKTLSWNCRGLGNPSAVRALLRLTRSENPQVVFLIETKLKSFEMEVIRRKSGFDACLSMD 532
           M  LSWN RGLGNP  VRAL  L R E+P V+FL ETKL    ME ++ + G+  CL++ 
Sbjct: 1   MSLLSWNTRGLGNPRGVRALSDLVREEDPMVLFLQETKLSEKGMEKLKYRLGYKNCLAVS 60

Query: 531 YVGNGRERAGGIALLWKESMNISVISYSLNHIQCSIVDDEDGGN-WFFIGVYGFPCEQDK 355
             G    R+GGIALLWK  +NI + +YS +HI  ++ D+    + WFF GVYG P    +
Sbjct: 61  SEG----RSGGIALLWKNDVNIVIKNYSRSHIHATLQDNTTADDSWFFTGVYGQPDPSRR 116

Query: 354 WKTWDLIHSIGGSVSSAWICVGDLNDTIAASEKQGGNPRSQHQLGIGRQSIDELNLLDLG 175
            +TW+L+ S+       W+ +GD N+ ++ +EK GG  R+  Q+    + ID+  LLDLG
Sbjct: 117 HETWNLLRSLKVPSDKGWLLMGDFNEVLSPNEKCGGRIRTDKQIQAFSEVIDDCELLDLG 176

Query: 174 FEGYPFTWPNGREERDNIQARLDRALGNASAINRFSPIKVSHLPHFKSDHAVVMVQLE 1
           F+G PFTW N RE    I  RLDR L N      F    VSH     SDH  + +QL+
Sbjct: 177 FKGNPFTWCNKREPAQCISERLDRCLANLKWKALFKMASVSHGSVAYSDHVPIKLQLQ 234


>ref|XP_021726600.1| uncharacterized protein LOC110693734 [Chenopodium quinoa]
          Length = 308

 Score =  188 bits (478), Expect = 1e-50
 Identities = 98/234 (41%), Positives = 146/234 (62%)
 Frame = -1

Query: 702 LSWNCRGLGNPSAVRALLRLTRSENPQVVFLIETKLKSFEMEVIRRKSGFDACLSMDYVG 523
           LSWNCRGLG  + V AL R+   E PQV+FL ET+LK+FEME I++K  F +C  ++  G
Sbjct: 5   LSWNCRGLGRTATVNALKRIVFLERPQVMFLSETRLKAFEMEKIKQKIKFHSCFVVECDG 64

Query: 522 NGRERAGGIALLWKESMNISVISYSLNHIQCSIVDDEDGGNWFFIGVYGFPCEQDKWKTW 343
            GR+R+GG+ALLW+ ++++ V SYSLNH+  ++V   +   W F+ +YG P E++K+KT 
Sbjct: 65  EGRKRSGGLALLWQSTIDVVVSSYSLNHVD-ALVGANEHEEWRFMVIYGHPEEENKYKTG 123

Query: 342 DLIHSIGGSVSSAWICVGDLNDTIAASEKQGGNPRSQHQLGIGRQSIDELNLLDLGFEGY 163
            L+ S+  +   +W+  GDLN  + + EK+GG         I R+++D   L DLGF G+
Sbjct: 124 QLLESLRDTNEKSWLVEGDLNLMLHSHEKRGGRGFCFEVADILRKAVDRCQLEDLGFIGH 183

Query: 162 PFTWPNGREERDNIQARLDRALGNASAINRFSPIKVSHLPHFKSDHAVVMVQLE 1
            FTW N +    NIQ RLDR L N +    F    V+HL   +SDH  +++ ++
Sbjct: 184 EFTWTNNQGGPKNIQERLDRFLANHAWREVFMGSFVTHLTKRRSDHLPILLCIQ 237