BLASTX nr result
ID: Astragalus22_contig00016461
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Astragalus22_contig00016461
(486 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_020985607.1| DEAD-box ATP-dependent RNA helicase 31 isofo... 94 4e-19
ref|XP_015937674.1| DEAD-box ATP-dependent RNA helicase 31 isofo... 94 4e-19
gb|KYP56065.1| DEAD-box ATP-dependent RNA helicase 31 [Cajanus c... 92 1e-18
ref|XP_020226374.1| DEAD-box ATP-dependent RNA helicase 31-like ... 92 1e-18
ref|XP_007135243.1| hypothetical protein PHAVU_010G112800g [Phas... 89 2e-17
ref|XP_011659146.1| PREDICTED: DEAD-box ATP-dependent RNA helica... 88 3e-17
ref|XP_004516413.1| PREDICTED: DEAD-box ATP-dependent RNA helica... 87 8e-17
ref|XP_008461517.1| PREDICTED: DEAD-box ATP-dependent RNA helica... 87 8e-17
ref|XP_022018017.1| DEAD-box ATP-dependent RNA helicase 31-like ... 87 1e-16
gb|KHN06301.1| DEAD-box ATP-dependent RNA helicase 31 [Glycine s... 86 1e-16
ref|XP_003529893.1| PREDICTED: DEAD-box ATP-dependent RNA helica... 86 1e-16
ref|XP_014624517.1| PREDICTED: DEAD-box ATP-dependent RNA helica... 86 1e-16
ref|XP_003548422.1| PREDICTED: DEAD-box ATP-dependent RNA helica... 86 1e-16
ref|XP_007218917.2| DEAD-box ATP-dependent RNA helicase 31 [Prun... 86 1e-16
ref|XP_008233195.1| PREDICTED: DEAD-box ATP-dependent RNA helica... 86 1e-16
gb|KCW71514.1| hypothetical protein EUGRSUZ_E000642, partial [Eu... 85 2e-16
ref|XP_013444648.1| DEAD-box ATP-dependent RNA helicase family p... 86 2e-16
ref|XP_014516672.1| DEAD-box ATP-dependent RNA helicase 31 [Vign... 86 2e-16
gb|PNY11375.1| DEAD-box ATP-dependent RNA helicase 31-like prote... 86 2e-16
ref|XP_015888691.1| PREDICTED: DEAD-box ATP-dependent RNA helica... 86 2e-16
>ref|XP_020985607.1| DEAD-box ATP-dependent RNA helicase 31 isoform X2 [Arachis
duranensis]
Length = 718
Score = 93.6 bits (231), Expect = 4e-19
Identities = 45/48 (93%), Positives = 47/48 (97%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP G+DSYLSDTRFDQCSISPLSLKGVKDAGYEKMT+VQAATLPVIL
Sbjct: 245 SSPSGTDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTIVQAATLPVIL 292
>ref|XP_015937674.1| DEAD-box ATP-dependent RNA helicase 31 isoform X1 [Arachis
duranensis]
Length = 725
Score = 93.6 bits (231), Expect = 4e-19
Identities = 45/48 (93%), Positives = 47/48 (97%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP G+DSYLSDTRFDQCSISPLSLKGVKDAGYEKMT+VQAATLPVIL
Sbjct: 245 SSPSGTDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTIVQAATLPVIL 292
Score = 60.8 bits (146), Expect = 1e-07
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 356 LLKYHPTIGVQVVIGGTRLALEQKRMQANPCQI 388
>gb|KYP56065.1| DEAD-box ATP-dependent RNA helicase 31 [Cajanus cajan]
Length = 668
Score = 92.4 bits (228), Expect = 1e-18
Identities = 46/48 (95%), Positives = 46/48 (95%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP GSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQ ATLPVIL
Sbjct: 188 SSPGGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQEATLPVIL 235
Score = 60.8 bits (146), Expect = 1e-07
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 299 LLKYHPTIGVQVVIGGTRLALEQKRMQANPCQI 331
>ref|XP_020226374.1| DEAD-box ATP-dependent RNA helicase 31-like [Cajanus cajan]
Length = 685
Score = 92.4 bits (228), Expect = 1e-18
Identities = 46/48 (95%), Positives = 46/48 (95%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP GSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQ ATLPVIL
Sbjct: 205 SSPGGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQEATLPVIL 252
Score = 60.8 bits (146), Expect = 1e-07
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 316 LLKYHPTIGVQVVIGGTRLALEQKRMQANPCQI 348
>ref|XP_007135243.1| hypothetical protein PHAVU_010G112800g [Phaseolus vulgaris]
gb|ESW07237.1| hypothetical protein PHAVU_010G112800g [Phaseolus vulgaris]
Length = 691
Score = 88.6 bits (218), Expect = 2e-17
Identities = 42/48 (87%), Positives = 46/48 (95%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP GSDSYLS++RFDQCS+SPLSLKG+KDAGYEKMTVVQ ATLPVIL
Sbjct: 210 SSPGGSDSYLSESRFDQCSVSPLSLKGIKDAGYEKMTVVQEATLPVIL 257
Score = 62.4 bits (150), Expect = 3e-08
Identities = 29/33 (87%), Positives = 31/33 (93%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH A+ VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 321 LLKYHPAIGVQVVIGGTRLALEQKRMQANPCQI 353
>ref|XP_011659146.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Cucumis
sativus]
ref|XP_011659147.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Cucumis
sativus]
ref|XP_011659148.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Cucumis
sativus]
gb|KGN44528.1| hypothetical protein Csa_7G325200 [Cucumis sativus]
Length = 787
Score = 88.2 bits (217), Expect = 3e-17
Identities = 42/48 (87%), Positives = 46/48 (95%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
+SP GSDSYLS+TRFDQCSISPLSLKG+KDAGYEKMTVVQ ATLP+IL
Sbjct: 308 TSPGGSDSYLSETRFDQCSISPLSLKGIKDAGYEKMTVVQEATLPLIL 355
Score = 61.6 bits (148), Expect = 5e-08
Identities = 28/34 (82%), Positives = 31/34 (91%)
Frame = +3
Query: 252 SLLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
+LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 417 TLLKYHSTIGVQVVIGGTRLALEQKRMQANPCQI 450
>ref|XP_004516413.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Cicer
arietinum]
Length = 687
Score = 87.0 bits (214), Expect = 8e-17
Identities = 42/48 (87%), Positives = 45/48 (93%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP G+ SYLSD+RFDQCS+SPLSLKGVKDAGYEKMTVVQ ATLPVIL
Sbjct: 207 SSPSGTASYLSDSRFDQCSVSPLSLKGVKDAGYEKMTVVQEATLPVIL 254
Score = 59.3 bits (142), Expect = 3e-07
Identities = 27/33 (81%), Positives = 29/33 (87%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH + VQVVIGGTRL LEQKRMQANPCQ+
Sbjct: 318 LLKYHPTIGVQVVIGGTRLTLEQKRMQANPCQI 350
>ref|XP_008461517.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Cucumis
melo]
ref|XP_008461520.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Cucumis
melo]
Length = 791
Score = 87.0 bits (214), Expect = 8e-17
Identities = 41/47 (87%), Positives = 45/47 (95%)
Frame = +2
Query: 113 SPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SP GSDSYLS+TRFDQCSISP+SLKG+KDAGYEKMTVVQ ATLP+IL
Sbjct: 313 SPGGSDSYLSETRFDQCSISPISLKGIKDAGYEKMTVVQEATLPLIL 359
Score = 61.6 bits (148), Expect = 5e-08
Identities = 28/34 (82%), Positives = 31/34 (91%)
Frame = +3
Query: 252 SLLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
+LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 421 TLLKYHSTIGVQVVIGGTRLALEQKRMQANPCQI 454
>ref|XP_022018017.1| DEAD-box ATP-dependent RNA helicase 31-like [Helianthus annuus]
gb|OTF92637.1| putative transcription-repair coupling factor [Helianthus annuus]
Length = 751
Score = 86.7 bits (213), Expect = 1e-16
Identities = 40/48 (83%), Positives = 46/48 (95%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP+GS+SYLS+TRFDQC++SPLSLK +KDAGYEKMTVVQ ATLPVIL
Sbjct: 271 SSPDGSESYLSETRFDQCAVSPLSLKAIKDAGYEKMTVVQEATLPVIL 318
Score = 57.0 bits (136), Expect = 2e-06
Identities = 25/33 (75%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
L+KYH ++ VQVVIGGTRL LEQKRMQA+PCQ+
Sbjct: 382 LIKYHPSIGVQVVIGGTRLGLEQKRMQASPCQI 414
>gb|KHN06301.1| DEAD-box ATP-dependent RNA helicase 31 [Glycine soja]
Length = 544
Score = 86.3 bits (212), Expect = 1e-16
Identities = 45/49 (91%), Positives = 46/49 (93%), Gaps = 1/49 (2%)
Frame = +2
Query: 110 SSPEG-SDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP G SDSYLS+TRFDQCSISPLSLKGVKDAGYEKMTVVQ ATLPVIL
Sbjct: 62 SSPGGGSDSYLSETRFDQCSISPLSLKGVKDAGYEKMTVVQEATLPVIL 110
Score = 60.8 bits (146), Expect = 9e-08
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 174 LLKYHPTIGVQVVIGGTRLALEQKRMQANPCQI 206
>ref|XP_003529893.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Glycine
max]
gb|KRH47920.1| hypothetical protein GLYMA_07G056600 [Glycine max]
Length = 703
Score = 86.3 bits (212), Expect = 1e-16
Identities = 45/49 (91%), Positives = 46/49 (93%), Gaps = 1/49 (2%)
Frame = +2
Query: 110 SSPEG-SDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP G SDSYLS+TRFDQCSISPLSLKGVKDAGYEKMTVVQ ATLPVIL
Sbjct: 221 SSPGGGSDSYLSETRFDQCSISPLSLKGVKDAGYEKMTVVQEATLPVIL 269
Score = 60.8 bits (146), Expect = 1e-07
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 333 LLKYHPTIGVQVVIGGTRLALEQKRMQANPCQI 365
>ref|XP_014624517.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like isoform X2
[Glycine max]
Length = 789
Score = 86.3 bits (212), Expect = 1e-16
Identities = 45/49 (91%), Positives = 46/49 (93%), Gaps = 1/49 (2%)
Frame = +2
Query: 110 SSPEG-SDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP G SDSYLS+TRFDQCSISPLSLKGVKDAGYEKMTVVQ ATLPVIL
Sbjct: 307 SSPGGGSDSYLSETRFDQCSISPLSLKGVKDAGYEKMTVVQEATLPVIL 355
Score = 60.8 bits (146), Expect = 1e-07
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 419 LLKYHPTIGVQVVIGGTRLALEQKRMQANPCQI 451
>ref|XP_003548422.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like isoform X1
[Glycine max]
gb|KRH06491.1| hypothetical protein GLYMA_16G025700 [Glycine max]
Length = 806
Score = 86.3 bits (212), Expect = 1e-16
Identities = 45/49 (91%), Positives = 46/49 (93%), Gaps = 1/49 (2%)
Frame = +2
Query: 110 SSPEG-SDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP G SDSYLS+TRFDQCSISPLSLKGVKDAGYEKMTVVQ ATLPVIL
Sbjct: 324 SSPGGGSDSYLSETRFDQCSISPLSLKGVKDAGYEKMTVVQEATLPVIL 372
Score = 60.8 bits (146), Expect = 1e-07
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 436 LLKYHPTIGVQVVIGGTRLALEQKRMQANPCQI 468
>ref|XP_007218917.2| DEAD-box ATP-dependent RNA helicase 31 [Prunus persica]
gb|ONI23392.1| hypothetical protein PRUPE_2G186500 [Prunus persica]
Length = 834
Score = 86.3 bits (212), Expect = 1e-16
Identities = 41/48 (85%), Positives = 45/48 (93%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP SDSYLS++RFDQCS+SPLSLKG+KDAGYEKMTVVQ ATLPVIL
Sbjct: 354 SSPGSSDSYLSESRFDQCSVSPLSLKGIKDAGYEKMTVVQEATLPVIL 401
Score = 59.7 bits (143), Expect = 3e-07
Identities = 27/33 (81%), Positives = 31/33 (93%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH ++ VQVVIGGTRLALEQKR+QANPCQ+
Sbjct: 465 LLKYHPSIGVQVVIGGTRLALEQKRIQANPCQI 497
>ref|XP_008233195.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Prunus
mume]
Length = 834
Score = 86.3 bits (212), Expect = 1e-16
Identities = 41/48 (85%), Positives = 45/48 (93%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP SDSYLS++RFDQCS+SPLSLKG+KDAGYEKMTVVQ ATLPVIL
Sbjct: 354 SSPGSSDSYLSESRFDQCSVSPLSLKGIKDAGYEKMTVVQEATLPVIL 401
Score = 59.7 bits (143), Expect = 3e-07
Identities = 27/33 (81%), Positives = 31/33 (93%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH ++ VQVVIGGTRLALEQKR+QANPCQ+
Sbjct: 465 LLKYHPSIGVQVVIGGTRLALEQKRIQANPCQI 497
>gb|KCW71514.1| hypothetical protein EUGRSUZ_E000642, partial [Eucalyptus grandis]
Length = 346
Score = 85.1 bits (209), Expect = 2e-16
Identities = 41/47 (87%), Positives = 44/47 (93%)
Frame = +2
Query: 113 SPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
S + SDSYLS+TRFDQCSISPLSLKG+KDAGYEKMTVVQ ATLPVIL
Sbjct: 299 SQQSSDSYLSETRFDQCSISPLSLKGIKDAGYEKMTVVQEATLPVIL 345
>ref|XP_013444648.1| DEAD-box ATP-dependent RNA helicase family protein [Medicago
truncatula]
gb|KEH18673.1| DEAD-box ATP-dependent RNA helicase family protein [Medicago
truncatula]
Length = 668
Score = 85.9 bits (211), Expect = 2e-16
Identities = 41/48 (85%), Positives = 45/48 (93%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
+S GSDSYLSD+RFDQCS+SPLSLKG+KDAGYEKMTVVQ ATLPVIL
Sbjct: 188 ASSGGSDSYLSDSRFDQCSVSPLSLKGIKDAGYEKMTVVQEATLPVIL 235
Score = 60.8 bits (146), Expect = 1e-07
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 299 LLKYHPTIGVQVVIGGTRLALEQKRMQANPCQI 331
>ref|XP_014516672.1| DEAD-box ATP-dependent RNA helicase 31 [Vigna radiata var. radiata]
Length = 691
Score = 85.9 bits (211), Expect = 2e-16
Identities = 42/48 (87%), Positives = 45/48 (93%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP GSDSYLS++RFDQCS+S LSLKGVKDAGYEKMTVVQ ATLPVIL
Sbjct: 210 SSPGGSDSYLSESRFDQCSVSQLSLKGVKDAGYEKMTVVQEATLPVIL 257
Score = 60.8 bits (146), Expect = 1e-07
Identities = 28/33 (84%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH + VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 321 LLKYHPTIGVQVVIGGTRLALEQKRMQANPCQI 353
>gb|PNY11375.1| DEAD-box ATP-dependent RNA helicase 31-like protein [Trifolium
pratense]
Length = 744
Score = 85.9 bits (211), Expect = 2e-16
Identities = 41/48 (85%), Positives = 45/48 (93%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
+S GSDSYLSD+RFDQCS+SPLSLKG+KDAGYEKMTVVQ ATLPVIL
Sbjct: 264 ASSGGSDSYLSDSRFDQCSVSPLSLKGIKDAGYEKMTVVQEATLPVIL 311
Score = 61.6 bits (148), Expect = 5e-08
Identities = 29/33 (87%), Positives = 30/33 (90%)
Frame = +3
Query: 255 LLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
LLKYH L VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 375 LLKYHPTLGVQVVIGGTRLALEQKRMQANPCQI 407
>ref|XP_015888691.1| PREDICTED: DEAD-box ATP-dependent RNA helicase 31-like [Ziziphus
jujuba]
Length = 790
Score = 85.9 bits (211), Expect = 2e-16
Identities = 42/48 (87%), Positives = 44/48 (91%)
Frame = +2
Query: 110 SSPEGSDSYLSDTRFDQCSISPLSLKGVKDAGYEKMTVVQAATLPVIL 253
SSP SDSYLS TRFDQCS+SPLSLKG+KDAGYEKMTVVQ ATLPVIL
Sbjct: 310 SSPNKSDSYLSYTRFDQCSVSPLSLKGIKDAGYEKMTVVQEATLPVIL 357
Score = 61.6 bits (148), Expect = 5e-08
Identities = 28/34 (82%), Positives = 32/34 (94%)
Frame = +3
Query: 252 SLLKYHHALSVQVVIGGTRLALEQKRMQANPCQL 353
+LLKYH ++ VQVVIGGTRLALEQKRMQANPCQ+
Sbjct: 420 TLLKYHPSIGVQVVIGGTRLALEQKRMQANPCQI 453