BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00013798
         (765 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003621600.1| PPR containing plant protein [Medicago trunc...   142   8e-35
ref|XP_004491961.1| PREDICTED: pentatricopeptide repeat-containi...   139   1e-33
ref|XP_003621596.1| PPR containing plant protein [Medicago trunc...   137   4e-33
gb|PNX96426.1| pentatricopeptide repeat-containing protein chlor...   136   1e-32
ref|XP_019452137.1| PREDICTED: pentatricopeptide repeat-containi...   118   2e-26
ref|XP_003551784.1| PREDICTED: pentatricopeptide repeat-containi...   112   3e-24
ref|XP_014497993.1| pentatricopeptide repeat-containing protein ...   110   9e-24
ref|XP_003531893.1| PREDICTED: pentatricopeptide repeat-containi...   109   2e-23
ref|XP_020212397.1| pentatricopeptide repeat-containing protein ...   108   3e-23
ref|XP_017417958.1| PREDICTED: pentatricopeptide repeat-containi...   108   3e-23
ref|XP_017417959.1| PREDICTED: pentatricopeptide repeat-containi...   108   3e-23
dbj|BAT83189.1| hypothetical protein VIGAN_04030300 [Vigna angul...   108   3e-23
ref|XP_007139379.1| hypothetical protein PHAVU_008G024400g [Phas...   107   8e-23
dbj|GAU14054.1| hypothetical protein TSUD_168790 [Trifolium subt...   100   2e-20
ref|XP_015962585.2| LOW QUALITY PROTEIN: pentatricopeptide repea...   100   3e-20
ref|XP_016194466.1| pentatricopeptide repeat-containing protein ...   100   3e-20
dbj|GAV83059.1| PPR domain-containing protein/PPR_2 domain-conta...    99   1e-19
gb|PON61744.1| Smr domain containing protein [Parasponia anderso...    96   7e-19
gb|PON45009.1| Smr domain containing protein [Trema orientalis]        95   2e-18
ref|XP_010087216.1| pentatricopeptide repeat-containing protein ...    94   3e-18

>ref|XP_003621600.1| PPR containing plant protein [Medicago truncatula]
 gb|AES77818.1| PPR containing plant protein [Medicago truncatula]
          Length = 890

 Score =  142 bits (358), Expect = 8e-35
 Identities = 67/109 (61%), Positives = 85/109 (77%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           R+S+LAPEFSG++STRFAAKMHSG PR+ PNKH H+ AA+EAL CL KAG++  AIDN+ 
Sbjct: 107 RQSRLAPEFSGRKSTRFAAKMHSGMPRVTPNKHAHSAAADEALSCLFKAGNNIAAIDNVF 166

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFNDDERVPKG 1
            SYEHKLW  +DYIY+LKEF N+  LL A+KC++F +     + RV KG
Sbjct: 167 ISYEHKLWEVEDYIYMLKEFGNTRSLLHAKKCFDFIMS--KQNGRVDKG 213


>ref|XP_004491961.1| PREDICTED: pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic [Cicer arietinum]
          Length = 909

 Score =  139 bits (349), Expect = 1e-33
 Identities = 66/109 (60%), Positives = 85/109 (77%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           R+S+LAPEFSG+RSTR+AAKMHSG PRI PN+HPH+EAA+E L+CL      S +IDNIL
Sbjct: 133 RESRLAPEFSGRRSTRYAAKMHSGMPRITPNRHPHSEAADEVLNCLFNCCSTSASIDNIL 192

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFNDDERVPKG 1
           F+YEHK+   +DYIY+LKEF N+GH L A KC++F   ++  D RV +G
Sbjct: 193 FTYEHKM-DIEDYIYILKEFGNTGHYLLASKCFDF--AMWKHDGRVARG 238


>ref|XP_003621596.1| PPR containing plant protein [Medicago truncatula]
 gb|AES77814.1| PPR containing plant protein [Medicago truncatula]
          Length = 849

 Score =  137 bits (345), Expect = 4e-33
 Identities = 65/109 (59%), Positives = 84/109 (77%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           R+S+LAPEFSG+RSTRFAAKMHSG PR+ PNKH H+ AA+EAL  L  AG++  AIDN+L
Sbjct: 72  RQSRLAPEFSGRRSTRFAAKMHSGMPRVTPNKHAHSAAADEALSYLFNAGNNIAAIDNVL 131

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFNDDERVPKG 1
            +YE +LW  +DYIY+LKEF N+GH L A KC++F   I+  + R+ KG
Sbjct: 132 IAYESELWEVEDYIYMLKEFGNTGHFLLATKCFDF--IIWKQNGRIAKG 178


>gb|PNX96426.1| pentatricopeptide repeat-containing protein chloroplastic-like
           [Trifolium pratense]
          Length = 879

 Score =  136 bits (342), Expect = 1e-32
 Identities = 64/109 (58%), Positives = 84/109 (77%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           R+S+LAPEFSG+R+TRF+AKMHSG PR+ PNKH H++ A+EAL CL KAG++  AIDN+L
Sbjct: 102 RQSRLAPEFSGRRTTRFSAKMHSGMPRVTPNKHAHSDVADEALRCLFKAGNNIAAIDNVL 161

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFNDDERVPKG 1
             YE KL   +DYIY++KEF N+GH L A KC++F   I+  + RV KG
Sbjct: 162 IEYEPKLRKVEDYIYMIKEFGNTGHFLLATKCFDF--IIWKQNGRVAKG 208


>ref|XP_019452137.1| PREDICTED: pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic [Lupinus angustifolius]
 gb|OIW07275.1| hypothetical protein TanjilG_08390 [Lupinus angustifolius]
          Length = 910

 Score =  118 bits (295), Expect = 2e-26
 Identities = 57/102 (55%), Positives = 76/102 (74%)
 Frame = -1

Query: 330 ARKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNI 151
           ARK+ L  EFSG+RSTRF +KM+S + R   NK+ H++ A+EAL CL+KAG+D  AIDN+
Sbjct: 119 ARKTGLGHEFSGRRSTRFVSKMYSVQTRASSNKNYHSDVADEALRCLVKAGNDCSAIDNV 178

Query: 150 LFSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFN 25
           L S+E++L   +DYIYLLKEF N G  L A KCY+F + I+N
Sbjct: 179 LLSFENRLVRVEDYIYLLKEFVNEGTYLLANKCYDFAMAIWN 220


>ref|XP_003551784.1| PREDICTED: pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic-like [Glycine max]
 gb|KRH01436.1| hypothetical protein GLYMA_18G276500 [Glycine max]
          Length = 875

 Score =  112 bits (279), Expect = 3e-24
 Identities = 55/104 (52%), Positives = 74/104 (71%)
 Frame = -1

Query: 330 ARKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNI 151
           A+K++LAPEFSG+ S R   KM+SG PR +PN   H++AAEE LH L  AG+D  AIDN+
Sbjct: 84  AQKTRLAPEFSGRPSNRNPGKMNSGGPRAVPNNQQHSKAAEEVLHSLTNAGNDVAAIDNV 143

Query: 150 LFSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFNDD 19
           L +Y  +L+  +DY+YLLKEFAN+G LL A + YNF +    D+
Sbjct: 144 LLNY--RLYVAEDYVYLLKEFANTGDLLLATRTYNFAMSRATDN 185


>ref|XP_014497993.1| pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic [Vigna radiata var. radiata]
          Length = 876

 Score =  110 bits (275), Expect = 9e-24
 Identities = 54/102 (52%), Positives = 73/102 (71%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           +K+QLAPEFSG+RS R   KM+SG PR +PN   H++AAEE LH L  AG+D  AID++L
Sbjct: 87  QKTQLAPEFSGRRSNRNPGKMNSGGPRAVPNNQQHSKAAEEVLHSLTNAGNDVAAIDSVL 146

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFND 22
            +Y  +L+  +DY+YLLKEFAN+G LL A + Y+F +    D
Sbjct: 147 LNY--RLYVAEDYVYLLKEFANTGDLLLATRTYDFAMSRATD 186


>ref|XP_003531893.1| PREDICTED: pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic-like [Glycine max]
 gb|KRH45156.1| hypothetical protein GLYMA_08G254000 [Glycine max]
          Length = 878

 Score =  109 bits (272), Expect = 2e-23
 Identities = 54/104 (51%), Positives = 74/104 (71%)
 Frame = -1

Query: 330 ARKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNI 151
           A+K++LAPEFSG+RS R   KM+SG PR +PN   H++AAEE LH L  AG+D  AID++
Sbjct: 85  AQKTRLAPEFSGRRSNRNPGKMNSGGPRAVPNNQQHSKAAEEVLHSLTNAGNDVSAIDSV 144

Query: 150 LFSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFNDD 19
           L  Y  +L+  +DY+YLLKEFAN+G LL A + Y+F +    D+
Sbjct: 145 LLHY--RLYVAEDYVYLLKEFANTGDLLLATRTYDFAMSRATDN 186


>ref|XP_020212397.1| pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic [Cajanus cajan]
          Length = 872

 Score =  108 bits (271), Expect = 3e-23
 Identities = 53/102 (51%), Positives = 73/102 (71%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           +K++LAP+FSG+RS+R   KM+SG PR +PN   H++AAEE LH L  AG+D  AID++L
Sbjct: 88  QKTRLAPDFSGRRSSRNPGKMNSGGPRAVPNNQQHSKAAEEVLHSLTNAGNDVAAIDSVL 147

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFND 22
            SY  +L+  +DY+YLLKEFAN+G LL A + Y F +    D
Sbjct: 148 LSY--RLYVAEDYVYLLKEFANTGDLLLATRTYEFAMSRATD 187


>ref|XP_017417958.1| PREDICTED: pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic isoform X1 [Vigna angularis]
          Length = 875

 Score =  108 bits (271), Expect = 3e-23
 Identities = 53/102 (51%), Positives = 72/102 (70%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           +K+QLAPEFSG+RS R   KM+SG PR +PN   H++ AEE LH L  AG+D  AID++L
Sbjct: 86  QKTQLAPEFSGRRSNRNPGKMNSGGPRAVPNNQQHSKGAEEVLHSLTNAGNDVAAIDSVL 145

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFND 22
            +Y  +L+  +DY+YLLKEFAN+G LL A + Y+F +    D
Sbjct: 146 LNY--RLYVAEDYVYLLKEFANTGDLLLATRTYDFAMSRATD 185


>ref|XP_017417959.1| PREDICTED: pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic isoform X2 [Vigna angularis]
 gb|KOM36654.1| hypothetical protein LR48_Vigan03g003500 [Vigna angularis]
          Length = 875

 Score =  108 bits (271), Expect = 3e-23
 Identities = 53/102 (51%), Positives = 72/102 (70%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           +K+QLAPEFSG+RS R   KM+SG PR +PN   H++ AEE LH L  AG+D  AID++L
Sbjct: 86  QKTQLAPEFSGRRSNRNPGKMNSGGPRAVPNNQQHSKGAEEVLHSLTNAGNDVAAIDSVL 145

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFND 22
            +Y  +L+  +DY+YLLKEFAN+G LL A + Y+F +    D
Sbjct: 146 LNY--RLYVAEDYVYLLKEFANTGDLLLATRTYDFAMSRATD 185


>dbj|BAT83189.1| hypothetical protein VIGAN_04030300 [Vigna angularis var.
           angularis]
          Length = 876

 Score =  108 bits (271), Expect = 3e-23
 Identities = 53/102 (51%), Positives = 72/102 (70%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           +K+QLAPEFSG+RS R   KM+SG PR +PN   H++ AEE LH L  AG+D  AID++L
Sbjct: 87  QKTQLAPEFSGRRSNRNPGKMNSGGPRAVPNNQQHSKGAEEVLHSLTNAGNDVAAIDSVL 146

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFND 22
            +Y  +L+  +DY+YLLKEFAN+G LL A + Y+F +    D
Sbjct: 147 LNY--RLYVAEDYVYLLKEFANTGDLLLATRTYDFAMSRATD 186


>ref|XP_007139379.1| hypothetical protein PHAVU_008G024400g [Phaseolus vulgaris]
 gb|ESW11373.1| hypothetical protein PHAVU_008G024400g [Phaseolus vulgaris]
          Length = 874

 Score =  107 bits (268), Expect = 8e-23
 Identities = 52/102 (50%), Positives = 73/102 (71%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           +K++LAPEFSG+RS +   KM+SG PR +PN   H++AAEE LH L  AG+D  AID++L
Sbjct: 85  QKTRLAPEFSGRRSNKNPGKMNSGGPRAVPNNQQHSKAAEEVLHSLTNAGNDVAAIDSVL 144

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIGIFND 22
            +Y  +L+  +DY+YLLKEFAN+G LL A + Y+F +    D
Sbjct: 145 LNY--RLYVAEDYVYLLKEFANTGDLLLATRTYDFAMSRATD 184


>dbj|GAU14054.1| hypothetical protein TSUD_168790 [Trifolium subterraneum]
          Length = 751

 Score =  100 bits (250), Expect = 2e-20
 Identities = 49/90 (54%), Positives = 64/90 (71%), Gaps = 1/90 (1%)
 Frame = -1

Query: 267 MHSGRPRIIPNKHPHTEAAEEALHCLLK-AGDDSVAIDNILFSYEHKLWGCDDYIYLLKE 91
           MHSG PR+ PNKH H++ A+EAL CL K AG++  AIDN+L  YE KL   +DYIY++KE
Sbjct: 1   MHSGMPRVTPNKHAHSDVADEALRCLFKDAGNNIAAIDNVLIEYEPKLRKVEDYIYMIKE 60

Query: 90  FANSGHLLQAEKCYNFGIGIFNDDERVPKG 1
           F N+GH L A KC++F   I+  + RV KG
Sbjct: 61  FGNTGHFLLATKCFDF--VIWKQNGRVAKG 88


>ref|XP_015962585.2| LOW QUALITY PROTEIN: pentatricopeptide repeat-containing protein
           At2g31400, chloroplastic [Arachis duranensis]
          Length = 869

 Score =  100 bits (249), Expect = 3e-20
 Identities = 49/98 (50%), Positives = 66/98 (67%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           R+S L  EFSG+R+TRFAA+M SGR R   NK  H+  AEE   CL K+G+D  ++DN+L
Sbjct: 118 RRSTLGLEFSGRRTTRFAARMRSGRLRYNNNKTKHSLIAEEVQQCLEKSGNDVASVDNVL 177

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIG 34
            SYE K++  +DYIYL++E  N    LQ  K Y+F +G
Sbjct: 178 LSYESKMYDPEDYIYLIRECGNKDLYLQVSKTYDFAMG 215


>ref|XP_016194466.1| pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic [Arachis ipaensis]
 ref|XP_016194467.1| pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic [Arachis ipaensis]
          Length = 902

 Score =  100 bits (249), Expect = 3e-20
 Identities = 49/98 (50%), Positives = 66/98 (67%)
 Frame = -1

Query: 327 RKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNIL 148
           R+S L  EFSG+R+TRFAA+M SGR R   NK  H+  AEE   CL K+G+D  ++DN+L
Sbjct: 118 RRSTLGLEFSGRRTTRFAARMRSGRLRYNNNKTKHSLIAEEVQQCLEKSGNDVASVDNVL 177

Query: 147 FSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGIG 34
            SYE K++  +DYIYL++E  N    LQ  K Y+F +G
Sbjct: 178 LSYESKMYDPEDYIYLIRECGNKDLYLQVSKTYDFAMG 215


>dbj|GAV83059.1| PPR domain-containing protein/PPR_2 domain-containing protein
           [Cephalotus follicularis]
          Length = 909

 Score = 98.6 bits (244), Expect = 1e-19
 Identities = 49/96 (51%), Positives = 65/96 (67%), Gaps = 1/96 (1%)
 Frame = -1

Query: 321 SQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHP-HTEAAEEALHCLLKAGDDSVAIDNILF 145
           S LA EFSG+RSTRF +K H GRP+  PN    HT AAE+ALH +L+ G D  A+DN+L 
Sbjct: 124 SDLAHEFSGRRSTRFLSKQHLGRPK--PNSSSRHTSAAEDALHEVLRCGRDLRALDNVLL 181

Query: 144 SYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGI 37
           +++  L GCDDY YL +E  + G  L+A KC+ F +
Sbjct: 182 NFQSLLSGCDDYTYLFRELGSRGECLKALKCFEFAV 217


>gb|PON61744.1| Smr domain containing protein [Parasponia andersonii]
          Length = 871

 Score = 96.3 bits (238), Expect = 7e-19
 Identities = 47/98 (47%), Positives = 63/98 (64%)
 Frame = -1

Query: 330 ARKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNI 151
           A KS+LA  FSG+RSTRF +KMH GRP+       HT  AEEAL   ++ G D + +D++
Sbjct: 83  APKSELATVFSGRRSTRFVSKMHWGRPKTTVGSR-HTSVAEEALQQAIQFGKDDMGLDDV 141

Query: 150 LFSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGI 37
           L S+EHKL G DDY +LL+E  N G   +A  C+ F +
Sbjct: 142 LLSFEHKLCGSDDYTFLLRELGNRGECRKAILCFEFAV 179


>gb|PON45009.1| Smr domain containing protein [Trema orientalis]
          Length = 869

 Score = 95.1 bits (235), Expect = 2e-18
 Identities = 47/98 (47%), Positives = 63/98 (64%)
 Frame = -1

Query: 330 ARKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNI 151
           A KS+LA  FSG+RSTRF +KMH GRP+       HT  AEEAL   ++ G D V +D++
Sbjct: 83  APKSELATVFSGRRSTRFVSKMHWGRPKTTVGSR-HTSVAEEALQQAIQFGKDDVGLDDV 141

Query: 150 LFSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGI 37
           L S+E KL G DDY +LL+E  N G   +A +C+ F +
Sbjct: 142 LLSFEPKLCGSDDYTFLLRELGNRGECRKAIRCFEFAV 179


>ref|XP_010087216.1| pentatricopeptide repeat-containing protein At2g31400,
           chloroplastic [Morus notabilis]
 gb|EXB28566.1| hypothetical protein L484_009725 [Morus notabilis]
          Length = 871

 Score = 94.4 bits (233), Expect = 3e-18
 Identities = 47/98 (47%), Positives = 61/98 (62%)
 Frame = -1

Query: 330 ARKSQLAPEFSGKRSTRFAAKMHSGRPRIIPNKHPHTEAAEEALHCLLKAGDDSVAIDNI 151
           A KS LA  FSG+RSTRF +KMH GRP+       HT  AEE L   ++ G D + IDN+
Sbjct: 84  APKSDLAAVFSGRRSTRFVSKMHLGRPKTTVGSR-HTAVAEEVLQQAIQFGKDDLGIDNV 142

Query: 150 LFSYEHKLWGCDDYIYLLKEFANSGHLLQAEKCYNFGI 37
           L S+E KL G DDY +LL+E  N G   +A +C+ F +
Sbjct: 143 LLSFEPKLCGSDDYTFLLRELGNRGECRKAIRCFEFAV 180