BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00013379
         (1509 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_001296643.1| isoflavone 2'-hydroxylase-like [Cicer arieti...   631   0.0  
dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]                630   0.0  
sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase;...   629   0.0  
dbj|BAT21113.1| cytochrome P450 [synthetic construct]                 625   0.0  
ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum] ...   625   0.0  
ref|XP_004504637.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   625   0.0  
ref|XP_004504638.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   624   0.0  
ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago ...   617   0.0  
gb|AHG97656.1| isoflavone-2'-hydroxylase [Astragalus mongholicus]     610   0.0  
dbj|BAT21114.1| cytochrome P450 [synthetic construct]                 590   0.0  
dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]                     591   0.0  
ref|XP_015957959.1| isoflavone 2'-hydroxylase [Arachis duranensis]    566   0.0  
ref|XP_016191270.1| isoflavone 2'-hydroxylase [Arachis ipaensis]      564   0.0  
ref|XP_017405614.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   562   0.0  
gb|KOM25514.1| hypothetical protein LR48_Vigan107s003300 [Vigna ...   562   0.0  
ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   559   0.0  
ref|XP_014506446.2| isoflavone 2'-hydroxylase [Vigna radiata var...   558   0.0  
ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phas...   558   0.0  
gb|KHN26592.1| Isoflavone 2'-hydroxylase [Glycine soja]               502   e-171
ref|XP_003533544.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   502   e-171

>ref|NP_001296643.1| isoflavone 2'-hydroxylase-like [Cicer arietinum]
 emb|CAB41490.1| cytochrome P450 monooxygenase [Cicer arietinum]
          Length = 498

 Score =  631 bits (1627), Expect = 0.0
 Identities = 312/400 (78%), Positives = 348/400 (87%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+FQSRKFK               HHLKRPL  TFKGLS+ YGDI+SLWFGSRLVV+VSS
Sbjct: 22   LLFQSRKFKNLPPGPPSLPIIGNFHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSS 81

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS SY E WRNLRRIT+LDVLSN RI
Sbjct: 82   LSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRI 141

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            N+FSG+R+DETQRLITKLAEDSST F EVELS RFYDMTFNNIMRMISGKRYYGED D+ 
Sbjct: 142  NNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMTFNNIMRMISGKRYYGEDCDMS 201

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +LQEAS+FRDM+ E+LQL+G NNKTDF+PLL F DFENLE ++K I++K DAFLRGLLQE
Sbjct: 202  DLQEASQFRDMVTELLQLSGANNKTDFMPLLKFFDFENLEKRVKNIADKTDAFLRGLLQE 261

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
            QR+KKERTNTMIDHLL+LQESQPEYYTDQIIKGLALAMLLAGTDSSAVTL+WS+S+LLN+
Sbjct: 262  QRNKKERTNTMIDHLLNLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNH 321

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PEVLKKVRDELDTHVG DRLV+ESDLPKLTYLKNVI ET            HSTSD+CII
Sbjct: 322  PEVLKKVRDELDTHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCII 381

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GGYKVPRDTIV++NAWAI RDP+ WSEATTFKPERF+K+G
Sbjct: 382  GGYKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKG 421


>dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  630 bits (1624), Expect = 0.0
 Identities = 310/401 (77%), Positives = 350/401 (87%)
 Frame = +3

Query: 306  NLIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVS 485
            N++ ++RKFK              LHHLKRPL  TFKGLSEKYG ++SLWFGSRLVV+VS
Sbjct: 21   NIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVISLWFGSRLVVVVS 80

Query: 486  SQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQR 665
            S + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS SYGE WRNLRRITALDVLSN R
Sbjct: 81   SASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHR 140

Query: 666  INSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDV 845
            INSFSGIR+DETQRLIT+LA+DSST+F E+ELS RFYDMTFNNIMRMISGKRYYGED D+
Sbjct: 141  INSFSGIRRDETQRLITRLADDSSTNFAEIELSYRFYDMTFNNIMRMISGKRYYGEDCDM 200

Query: 846  LELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQ 1025
             +LQEAS+FRDM++E+LQL+G NNKTDF+PLL FLDFENLE +LK IS K DAFLRGL+Q
Sbjct: 201  SDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIQ 260

Query: 1026 EQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLN 1205
            E R KKER NTMIDHLL+LQ+SQPEYYTDQIIKGLALAMLLAGTDSSAVTL+WS+S+LLN
Sbjct: 261  EHRAKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLN 320

Query: 1206 NPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECI 1385
            +PEVLKKV+DELDTHVG DRLV+ESDLPKL+YLKNVINET            HSTSDEC 
Sbjct: 321  HPEVLKKVKDELDTHVGQDRLVDESDLPKLSYLKNVINETLRLYTPAPLLLPHSTSDECN 380

Query: 1386 IGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            IGGYKVP+DTIV++NAWAI RDPELW+EATTFKPERFEK+G
Sbjct: 381  IGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKG 421


>sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase; AltName: Full=CYP GE-3;
            AltName: Full=Cytochrome P450 81E1; AltName:
            Full=Cytochrome P450 91A4
 dbj|BAA22422.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  629 bits (1622), Expect = 0.0
 Identities = 310/401 (77%), Positives = 348/401 (86%)
 Frame = +3

Query: 306  NLIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVS 485
            N++ ++RKFK              LHHLKRPL  TFKGLSEKYG + SLWFGSRLVV+VS
Sbjct: 21   NIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVS 80

Query: 486  SQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQR 665
            S + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS SYGE WRNLRRITALDVLSN R
Sbjct: 81   SASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHR 140

Query: 666  INSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDV 845
            INSFSGIR+DETQRLIT+LA+DSST+F E+ELSSR YDMTFNNIMRMISGKRYYGED D 
Sbjct: 141  INSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDT 200

Query: 846  LELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQ 1025
             +LQEAS+FRDM++E+LQL+G NNKTDF+PLL FLDFENLE +LK IS K DAFLRGL++
Sbjct: 201  SDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIE 260

Query: 1026 EQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLN 1205
            E R KKER NTMIDHLL+LQ+SQPEYYTDQIIKGLALAMLLAGTDSSAVTL+WS+S+LLN
Sbjct: 261  EHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLN 320

Query: 1206 NPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECI 1385
            +PEVLKKV+DELDTHVG DRLV+ESDLPKLTYLKNVINET            HSTSDEC 
Sbjct: 321  HPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECN 380

Query: 1386 IGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            IGGYKVP+DTIV++NAWAI RDPELW+EATTFKPERFEK+G
Sbjct: 381  IGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKG 421


>dbj|BAT21113.1| cytochrome P450 [synthetic construct]
          Length = 477

 Score =  625 bits (1612), Expect = 0.0
 Identities = 309/399 (77%), Positives = 346/399 (86%)
 Frame = +3

Query: 312  IFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSSQ 491
            + ++RKFK              LHHLKRPL  TFKGLSEKYG + SLWFGSRLVV+VSS 
Sbjct: 1    MIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSA 60

Query: 492  AIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRIN 671
            + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS SYGE WRNLRRITALDVLSN RIN
Sbjct: 61   SEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRIN 120

Query: 672  SFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVLE 851
            SFSGIR+DETQRLIT+LA+DSST+F E+ELSSR YDMTFNNIMRMISGKRYYGED D  +
Sbjct: 121  SFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSD 180

Query: 852  LQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQEQ 1031
            LQEAS+FRDM++E+LQL+G NNKTDF+PLL FLDFENLE +LK IS K DAFLRGL++E 
Sbjct: 181  LQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEH 240

Query: 1032 RDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNNP 1211
            R KKER NTMIDHLL+LQ+SQPEYYTDQIIKGLALAMLLAGTDSSAVTL+WS+S+LLN+P
Sbjct: 241  RTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHP 300

Query: 1212 EVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECIIG 1391
            EVLKKV+DELDTHVG DRLV+ESDLPKLTYLKNVINET            HSTSDEC IG
Sbjct: 301  EVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIG 360

Query: 1392 GYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GYKVP+DTIV++NAWAI RDPELW+EATTFKPERFEK+G
Sbjct: 361  GYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKG 399


>ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum]
 emb|CAA10067.1| cytochrome P450 [Cicer arietinum]
          Length = 498

 Score =  625 bits (1612), Expect = 0.0
 Identities = 308/400 (77%), Positives = 347/400 (86%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+FQSRKFK              LHHLKRPL  TFKGLS+ YGDI+SLWFGSRLVV+VSS
Sbjct: 22   LLFQSRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSS 81

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS SY E WRNLRRIT+LDVLSN RI
Sbjct: 82   LSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRI 141

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            N+FSG+R+DETQRLITKLAEDSST F EVELS RFYDMTFNNIMRMI GKRYYGED D+ 
Sbjct: 142  NNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMTFNNIMRMIPGKRYYGEDCDMS 201

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +LQEAS+FRDM+ E+LQL+G NNKTDF+PLL F DFENLE ++K I++K DAFLRGLLQE
Sbjct: 202  DLQEASQFRDMVTELLQLSGANNKTDFMPLLKFFDFENLEKRVKNIADKTDAFLRGLLQE 261

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
            QR+KKERTNTMIDHLL+LQESQPEYYTDQIIKGLALAMLLAGTDSSAVTL+WS+S+LLN+
Sbjct: 262  QRNKKERTNTMIDHLLNLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNH 321

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PE+LKKV+DELD HVG DRLV+ESDLPKLTYLKNVI ET            HSTSD+CII
Sbjct: 322  PEILKKVKDELDIHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCII 381

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GGYKVPRDTIV++NAWAI RDP+ WSEAT+FKPERF+K+G
Sbjct: 382  GGYKVPRDTIVLINAWAIHRDPKSWSEATSFKPERFDKKG 421


>ref|XP_004504637.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
          Length = 511

 Score =  625 bits (1611), Expect = 0.0
 Identities = 307/400 (76%), Positives = 346/400 (86%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+FQSR+FK              LHHLKRPL  TFK L++KYGD++SLWFGSRLVV+VSS
Sbjct: 22   LLFQSRRFKNLPPGPPSLPIIGNLHHLKRPLHRTFKELTKKYGDVISLWFGSRLVVVVSS 81

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             + FQ+CFTK+DVVLANRPKF+SGKYIFYNYTTLGS SYGE WRNLRRIT+LDVLSN RI
Sbjct: 82   LSEFQQCFTKNDVVLANRPKFISGKYIFYNYTTLGSASYGEHWRNLRRITSLDVLSNHRI 141

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            N+FSGIRKDETQRLI KLAEDSS++F EVELS RFYDMTFNNIMRMISGKRYYGED D+ 
Sbjct: 142  NNFSGIRKDETQRLIKKLAEDSSSNFAEVELSFRFYDMTFNNIMRMISGKRYYGEDCDMS 201

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +LQEAS+FRDM+ E+LQL+G NNKTDF+PLL FLDFENLE K+K I EK DAFL GLLQE
Sbjct: 202  DLQEASQFRDMVTELLQLSGANNKTDFLPLLRFLDFENLEKKVKNIGEKTDAFLSGLLQE 261

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
            QR+KKERTNTMIDHLLS+QESQPEYYTDQIIKGL LAMLLAGTD++A+TL+WS+S+LLN+
Sbjct: 262  QRNKKERTNTMIDHLLSMQESQPEYYTDQIIKGLVLAMLLAGTDTTALTLEWSMSNLLNH 321

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PEVLKKVRDELDTHVG DRLV+ESDLP LTY+KNVI ET            HSTSD+CII
Sbjct: 322  PEVLKKVRDELDTHVGQDRLVDESDLPNLTYIKNVIYETLRLYGPAPLLIPHSTSDDCII 381

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GGYK+PRDTIV++NAW IQRDPE WSEATTFKPERF K+G
Sbjct: 382  GGYKIPRDTIVLINAWGIQRDPESWSEATTFKPERFNKKG 421


>ref|XP_004504638.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
          Length = 498

 Score =  624 bits (1609), Expect = 0.0
 Identities = 307/400 (76%), Positives = 345/400 (86%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+FQSRKFK              LHHLKRPL  TFKGLS+ YGDI+SLWFGSRLVV+VSS
Sbjct: 22   LLFQSRKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSKTYGDIISLWFGSRLVVVVSS 81

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             + FQ+CFTK+DVVLANRP+FLSGKYIFYNYTTLGS SY E WRNLRRIT+LDVLSN RI
Sbjct: 82   LSEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRI 141

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            N+FSG+R+DETQRLITKLAEDSST F EVELS RFYDMTFNNIMRMISGKRYYGED D+ 
Sbjct: 142  NNFSGVRRDETQRLITKLAEDSSTSFAEVELSFRFYDMTFNNIMRMISGKRYYGEDCDMS 201

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +LQEAS+FRDM+ E+LQL+G NNKTDF+PLL F DFENLE ++K I+ K D FLRGLLQE
Sbjct: 202  DLQEASQFRDMVTELLQLSGANNKTDFLPLLKFFDFENLEKRVKNIANKTDTFLRGLLQE 261

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
            QR+KKERTNTMIDHLL+LQESQPEYYTDQIIKGLALAMLLAGTDSSAVTL+WS+ +L+N+
Sbjct: 262  QRNKKERTNTMIDHLLNLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNLMNH 321

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PE+LKKV+DELD HVG DRLV+ESDLPKLTYLKNVI ET            HSTSD+CII
Sbjct: 322  PEILKKVKDELDIHVGQDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCII 381

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GGYKVPRDTIV++NAWAI RDP+ WSEATTFKPERF+K+G
Sbjct: 382  GGYKVPRDTIVLINAWAIHRDPKSWSEATTFKPERFDKKG 421


>ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago truncatula]
 sp|Q6WNR0.1|C81E7_MEDTR RecName: Full=Isoflavone 2'-hydroxylase; AltName:
            Full=4'-methoxyisoflavone 2'-hydroxylase; AltName:
            Full=Cytochrome P450 81E7
 gb|AAQ20040.1| isoflavone 2'-hydroxylase [Medicago truncatula]
 gb|KEH31433.1| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 498

 Score =  617 bits (1590), Expect = 0.0
 Identities = 302/400 (75%), Positives = 345/400 (86%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+FQSRKFK              LHHLKRPL  TFK L+EKYG+++SLWFGSRLVV+VSS
Sbjct: 22   LLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNVISLWFGSRLVVVVSS 81

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             + FQECFTK+DVVLANRP+FLSGKYIFYNYTTLGS SYGE WRNLRRIT+LDVLSN RI
Sbjct: 82   LSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRI 141

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            N+F+ IR+DETQRLI KLAEDSST F EVEL+ RF+DMTFNNIMRMISGKRYYG+D D+ 
Sbjct: 142  NNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMISGKRYYGDDCDIS 201

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            E+QEAS+FRDM++E+LQL+G NNKTDF+PLL FLDFENLE ++K+I EKND FL GLLQE
Sbjct: 202  EVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKRIGEKNDVFLSGLLQE 261

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
            QR KKERTNTMIDHLL++QESQPEYYTD IIKGL LAMLLAGTDSSAVTL+W++S++LN 
Sbjct: 262  QRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAGTDSSAVTLEWTMSNILNY 321

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PEVLKKVRDE+DTHVG DRLV+ESDLPKLTYL+NVI ET            HST+DECI+
Sbjct: 322  PEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLYTPAPLLLPHSTADECIM 381

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GGYKVPRDTIV++NAWAI RDPE WSEATTFKPERF+K+G
Sbjct: 382  GGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKG 421


>gb|AHG97656.1| isoflavone-2'-hydroxylase [Astragalus mongholicus]
          Length = 498

 Score =  610 bits (1574), Expect = 0.0
 Identities = 296/401 (73%), Positives = 340/401 (84%)
 Frame = +3

Query: 306  NLIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVS 485
            NLIFQSRKFK              LHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVS
Sbjct: 21   NLIFQSRKFKNLPPGPPSLPIIGNLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVS 80

Query: 486  SQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQR 665
            SQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGS SYGE WRNLRRITA DVLS  R
Sbjct: 81   SQAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNR 140

Query: 666  INSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDV 845
            INSF+GIR DE  RL+ KLAEDSS DFVEVEL+S+FYDMTFNNIMRMISGKRYYG+D D+
Sbjct: 141  INSFAGIRNDEIHRLVQKLAEDSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDM 200

Query: 846  LELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQ 1025
             +++EA++FR M++++L+L+G NNK DF+P+L  +D+ENLE +L KI +KNDAFLRGL++
Sbjct: 201  TDVEEATQFRAMVSDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIE 260

Query: 1026 EQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLN 1205
            E R KK+  NTM+DHLLSLQESQPEYYTDQIIKGL   +LLAGTDSSAVTL+W+LS +LN
Sbjct: 261  EHRSKKKHDNTMVDHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLN 320

Query: 1206 NPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECI 1385
            NPEVLKK RDELDTH+G DRL+EESDLPK+ YLKN++ ET            HS+S+ECI
Sbjct: 321  NPEVLKKTRDELDTHIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECI 380

Query: 1386 IGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            I GYKVPRDTI++ NAWAI RDP++WSEAT+FKPERF+KEG
Sbjct: 381  IAGYKVPRDTIILTNAWAIHRDPKVWSEATSFKPERFDKEG 421


>dbj|BAT21114.1| cytochrome P450 [synthetic construct]
          Length = 477

 Score =  590 bits (1522), Expect = 0.0
 Identities = 293/397 (73%), Positives = 333/397 (83%), Gaps = 1/397 (0%)
 Frame = +3

Query: 321  SRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSSQAIF 500
            SRKFK              LHHLKRPL  TF+ LSEKYGD+ SLWFG+RLVV+VSS A  
Sbjct: 4    SRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSSFADV 63

Query: 501  QECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRINSFS 680
            QECFTK+DVVLANRP+FLSGKYIFYNYTTLGS SYGE WRNLRRIT+LDVLSN RINSFS
Sbjct: 64   QECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRINSFS 123

Query: 681  GIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVLELQE 860
             IR+DET RLI KLAEDS+ +F EVEL+SRF+DMTFNNIMRMISGKRYYGED D+ ELQE
Sbjct: 124  PIRRDETTRLIRKLAEDSAKNFSEVELTSRFFDMTFNNIMRMISGKRYYGEDCDMTELQE 183

Query: 861  ASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQEQRDK 1040
            AS FRDM+ E+LQL+G NNK DF+P+L  +DFE LE ++K IS K D FLRGLLQE RDK
Sbjct: 184  ASEFRDMVTELLQLSGANNKADFMPILRLVDFEGLEKRVKGISSKTDRFLRGLLQEHRDK 243

Query: 1041 KERT-NTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNNPEV 1217
            K+RT NTMIDHLL+LQESQPEYYTDQIIKGLALAMLLAGTDSSAVTL+WS+ ++LN PEV
Sbjct: 244  KQRTANTMIDHLLTLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNVLNYPEV 303

Query: 1218 LKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECIIGGY 1397
            L+K++ ELDTHVG DRLV+ESD+PKLTYLKNVINET            HS SD+C IGGY
Sbjct: 304  LEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCTIGGY 363

Query: 1398 KVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            KVPRDTIV++NAWA+ RDP+LW+EATTFKPERF+K+G
Sbjct: 364  KVPRDTIVLINAWALHRDPQLWTEATTFKPERFDKKG 400


>dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]
          Length = 499

 Score =  591 bits (1524), Expect = 0.0
 Identities = 294/401 (73%), Positives = 334/401 (83%), Gaps = 1/401 (0%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L   SRKFK              LHHLKRPL  TF+ LSEKYGD+ SLWFG+RLVV+VSS
Sbjct: 22   LFLGSRKFKNLPPGPTSLPIIGNLHHLKRPLHRTFRALSEKYGDVFSLWFGNRLVVVVSS 81

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             A  QECFTK+DVVLANRP+FLSGKYIFYNYTTLGS SYGE WRNLRRIT+LDVLSN RI
Sbjct: 82   FADVQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRI 141

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            NSFS IR+DET RLI KLAEDS+ +F EVEL+SRF+DMTFNNIMRMISGKRYYGED D+ 
Sbjct: 142  NSFSPIRRDETTRLIRKLAEDSAKNFSEVELTSRFFDMTFNNIMRMISGKRYYGEDCDMT 201

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            ELQEAS FRDM+ E+LQL+G NNK DF+P+L  +DFE LE ++K IS K D FLRGLLQE
Sbjct: 202  ELQEASEFRDMVTELLQLSGANNKADFMPILRLVDFEGLEKRVKGISSKTDRFLRGLLQE 261

Query: 1029 QRDKKERT-NTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLN 1205
             RDKK+RT NTMIDHLL+LQESQPEYYTDQIIKGLALAMLLAGTDSSAVTL+WS+ ++LN
Sbjct: 262  HRDKKQRTANTMIDHLLTLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNVLN 321

Query: 1206 NPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECI 1385
             PEVL+K++ ELDTHVG DRLV+ESD+PKLTYLKNVINET            HS SD+C 
Sbjct: 322  YPEVLEKIKAELDTHVGQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCT 381

Query: 1386 IGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            IGGYKVPRDTIV++NAWA+ RDP+LW+EATTFKPERF+K+G
Sbjct: 382  IGGYKVPRDTIVLINAWALHRDPQLWTEATTFKPERFDKKG 422


>ref|XP_015957959.1| isoflavone 2'-hydroxylase [Arachis duranensis]
          Length = 499

 Score =  566 bits (1458), Expect = 0.0
 Identities = 273/400 (68%), Positives = 325/400 (81%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+ QSR FK              LHHLK+PL   F+GLSEKYGD+ SLWFGSRLVV+VSS
Sbjct: 22   LLIQSRSFKNLPPGPPSLPIIGNLHHLKKPLHRHFRGLSEKYGDVFSLWFGSRLVVVVSS 81

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
              + QECFTK+DVVLANRP+FLSGKYIFYNYTT+GS  YGE WRNLRRIT+LDVLSN R+
Sbjct: 82   PTVVQECFTKNDVVLANRPRFLSGKYIFYNYTTVGSSEYGEHWRNLRRITSLDVLSNHRL 141

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            NSFS IR+DET RLI KL+EDS+ +F +VELSS+FYDMTFNNIMRMISGKRYYGED D+ 
Sbjct: 142  NSFSPIRRDETTRLIRKLSEDSTENFAKVELSSKFYDMTFNNIMRMISGKRYYGEDCDMA 201

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +++EA  FR MI+EVLQL+G NNKTDFIPLLG LD +NL+ +L  IS + D FL GLLQE
Sbjct: 202  DVEEAKHFRQMISEVLQLSGANNKTDFIPLLGMLDVDNLKKRLVTISGRTDKFLNGLLQE 261

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
             R K +  NTMIDHLLSLQ+SQP+YYTDQIIKGL LAMLLAGTDSSAVTL+WS+ +LLN+
Sbjct: 262  HRTKNQHQNTMIDHLLSLQDSQPDYYTDQIIKGLGLAMLLAGTDSSAVTLEWSMCNLLNH 321

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PE L +VR+ELDTHVG DR+V+ESD+PK TYL+NVINET            HS++++C I
Sbjct: 322  PEALNRVREELDTHVGQDRIVDESDIPKFTYLRNVINETLRLYTPAPLLLPHSSAEKCTI 381

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GGYK+P+DTIV++N WAI RDP+ W EAT+FKPERFE++G
Sbjct: 382  GGYKIPKDTIVLINGWAIHRDPKNWDEATSFKPERFERKG 421


>ref|XP_016191270.1| isoflavone 2'-hydroxylase [Arachis ipaensis]
          Length = 499

 Score =  564 bits (1454), Expect = 0.0
 Identities = 272/400 (68%), Positives = 325/400 (81%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+ QSR FK              LHHLK+PL   F+GLSEKYGD+ SLWFGSRLVV+VSS
Sbjct: 22   LLIQSRSFKNLPPGPPSLPIIGNLHHLKKPLHRHFRGLSEKYGDVFSLWFGSRLVVVVSS 81

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
              + QECFTK+DVVLANRP+FLSGKYIFYNYTT+GS  YGE WRNLRRIT+LDVLSN R+
Sbjct: 82   PTVVQECFTKNDVVLANRPRFLSGKYIFYNYTTVGSSEYGEHWRNLRRITSLDVLSNHRL 141

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            NSFS IR+DET RLI KL+EDS+ +F +VELSS+FYDMTFNNIMRMISGKRYYGED D+ 
Sbjct: 142  NSFSPIRRDETTRLIRKLSEDSTENFAKVELSSKFYDMTFNNIMRMISGKRYYGEDCDMA 201

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +++EA +FR MI+EVLQL+G NNKTDFIPLLG LD +NL+ +L  IS + D FL GLLQE
Sbjct: 202  DVEEAKQFRQMISEVLQLSGANNKTDFIPLLGMLDVDNLKKRLVTISGRTDKFLNGLLQE 261

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
             R K +  NTMIDHLLSLQ+SQP+YYTDQIIKGL LAMLLAGTDSSAVTL+WS+ +LLN+
Sbjct: 262  HRTKNQHQNTMIDHLLSLQDSQPDYYTDQIIKGLGLAMLLAGTDSSAVTLEWSMCNLLNH 321

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PE L +VR+ELDTHVG DR+V+ESD+PK TYL+NVINET            HS++++C I
Sbjct: 322  PEALNRVREELDTHVGQDRIVDESDIPKFTYLRNVINETLRLYTPAPLLLPHSSAEKCTI 381

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GGY +P+DTIV++N WAI RDP+ W EAT+FKPERFE++G
Sbjct: 382  GGYNIPKDTIVLINGWAIHRDPKNWDEATSFKPERFERKG 421


>ref|XP_017405614.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vigna angularis]
          Length = 500

 Score =  562 bits (1449), Expect = 0.0
 Identities = 270/400 (67%), Positives = 328/400 (82%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+ Q+RKF+              LHHLKRPL  TFK LS+ YG ++SLWFGSRLVV+VSS
Sbjct: 24   LLLQARKFQNLPPGPPSLPIFGNLHHLKRPLHRTFKALSDNYGHVISLWFGSRLVVVVSS 83

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             ++FQ CFTK+DV LANRP+FLSGKYIFYNYTTLGS  YG  WRNLRRITAL++LS  RI
Sbjct: 84   HSLFQHCFTKNDVALANRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTHRI 143

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            N+F+ IR+DET RLI KLAED+S+DF EVEL+S+FYDMTFNNIMRMISGKRYYG+D D+ 
Sbjct: 144  NTFADIRRDETHRLIRKLAEDASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMA 203

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +++EA +FR M++E+L+L+G NNK DF+PLL   DFENLE +LKKIS K D FL GLL+E
Sbjct: 204  DVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDFENLEKRLKKISSKTDTFLNGLLEE 263

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
            QR +K+  NTM+DHLLSLQESQP+YYTDQIIKGLAL ML+AGTDSSAVTL+W+LS +LN+
Sbjct: 264  QRSRKQGANTMVDHLLSLQESQPDYYTDQIIKGLALGMLVAGTDSSAVTLEWALSCMLNH 323

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PEVLK+ RDEL+  VG +RL+EESDLPKL YLKN+I ET            HS+S+EC+I
Sbjct: 324  PEVLKRTRDELEKQVGQERLLEESDLPKLPYLKNIIFETLRLYTPAPLLLPHSSSEECMI 383

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GG+KVPRD+IV++NAW+I RDPE+WSEAT+FKPERFE EG
Sbjct: 384  GGFKVPRDSIVLINAWSIHRDPEVWSEATSFKPERFESEG 423


>gb|KOM25514.1| hypothetical protein LR48_Vigan107s003300 [Vigna angularis]
 dbj|BAT74198.1| hypothetical protein VIGAN_01181700 [Vigna angularis var. angularis]
          Length = 504

 Score =  562 bits (1449), Expect = 0.0
 Identities = 270/400 (67%), Positives = 328/400 (82%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+ Q+RKF+              LHHLKRPL  TFK LS+ YG ++SLWFGSRLVV+VSS
Sbjct: 28   LLLQARKFQNLPPGPPSLPIFGNLHHLKRPLHRTFKALSDNYGHVISLWFGSRLVVVVSS 87

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             ++FQ CFTK+DV LANRP+FLSGKYIFYNYTTLGS  YG  WRNLRRITAL++LS  RI
Sbjct: 88   HSLFQHCFTKNDVALANRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTHRI 147

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            N+F+ IR+DET RLI KLAED+S+DF EVEL+S+FYDMTFNNIMRMISGKRYYG+D D+ 
Sbjct: 148  NTFADIRRDETHRLIRKLAEDASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMA 207

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +++EA +FR M++E+L+L+G NNK DF+PLL   DFENLE +LKKIS K D FL GLL+E
Sbjct: 208  DVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDFENLEKRLKKISSKTDTFLNGLLEE 267

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
            QR +K+  NTM+DHLLSLQESQP+YYTDQIIKGLAL ML+AGTDSSAVTL+W+LS +LN+
Sbjct: 268  QRSRKQGANTMVDHLLSLQESQPDYYTDQIIKGLALGMLVAGTDSSAVTLEWALSCMLNH 327

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PEVLK+ RDEL+  VG +RL+EESDLPKL YLKN+I ET            HS+S+EC+I
Sbjct: 328  PEVLKRTRDELEKQVGQERLLEESDLPKLPYLKNIIFETLRLYTPAPLLLPHSSSEECMI 387

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GG+KVPRD+IV++NAW+I RDPE+WSEAT+FKPERFE EG
Sbjct: 388  GGFKVPRDSIVLINAWSIHRDPEVWSEATSFKPERFESEG 427


>ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
 gb|KRH42431.1| hypothetical protein GLYMA_08G089500 [Glycine max]
          Length = 502

 Score =  559 bits (1440), Expect = 0.0
 Identities = 273/404 (67%), Positives = 327/404 (80%), Gaps = 4/404 (0%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+ Q+RKF+              LHHLKRPL  TF+ LS+KYG ++SLWFGSRLVV+VSS
Sbjct: 22   LLLQARKFQNLPPGPPSLPIIGNLHHLKRPLHRTFRALSDKYGHVISLWFGSRLVVVVSS 81

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
            Q +FQECFTK+DVVLANRP+FLSGK+IFYNYTTLGS  YGE WRNLRRITALDVLS  R+
Sbjct: 82   QTLFQECFTKNDVVLANRPRFLSGKHIFYNYTTLGSSPYGEHWRNLRRITALDVLSTHRL 141

Query: 669  NSFSGIRKDETQRLITKLAE----DSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGED 836
            +SF+ IR+DET RL+ KLAE    +SS  F EVEL+S+FYDMTFNNIMRMISGKRYYG+D
Sbjct: 142  HSFAAIRRDETHRLVRKLAEAQGSESSLSFAEVELTSKFYDMTFNNIMRMISGKRYYGDD 201

Query: 837  LDVLELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRG 1016
             D+ +++EA +FR M++E+L+L G NNK DF+P+L   DFENLE +LKKIS K D FLRG
Sbjct: 202  CDMADVEEAKQFRAMVSELLKLAGANNKNDFMPVLRLFDFENLEKRLKKISNKTDTFLRG 261

Query: 1017 LLQEQRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSS 1196
            LL+E R KK+R NTM+DHLLSLQESQPEYYTDQIIKGLAL ML+A TDS AVTL+W+LS 
Sbjct: 262  LLEEIRAKKQRANTMLDHLLSLQESQPEYYTDQIIKGLALGMLIAATDSQAVTLEWALSC 321

Query: 1197 LLNNPEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSD 1376
            +LN+PEV K+ RDEL+THVG D L+EESDL KL YLKN+I ET            HS+S+
Sbjct: 322  VLNHPEVFKRARDELETHVGQDHLLEESDLSKLPYLKNIIYETLRLYTPAPLLLPHSSSE 381

Query: 1377 ECIIGGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            ECIIGG+KVP DTIV++NAW+I RDP++WSEAT+FKPERFEKEG
Sbjct: 382  ECIIGGFKVPGDTIVLINAWSIHRDPKVWSEATSFKPERFEKEG 425


>ref|XP_014506446.2| isoflavone 2'-hydroxylase [Vigna radiata var. radiata]
          Length = 504

 Score =  558 bits (1438), Expect = 0.0
 Identities = 266/400 (66%), Positives = 328/400 (82%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+ Q+RKF+              LHHLKRPL  TFK LS+ YG ++SLWFGSRLVV+VSS
Sbjct: 28   LLLQARKFQNLPPGPPSLPVIGNLHHLKRPLHRTFKALSDNYGHVISLWFGSRLVVVVSS 87

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             ++FQ CFTK+D+ LANRP+FLSGKYIFYNYTTLGS  YG  WRNLRRITAL++LS  RI
Sbjct: 88   HSLFQHCFTKNDIALANRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTHRI 147

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            N+F+ IR+DET RLI KLAED+S+DF EVEL+S+FYDMTFNNIMRMISGKRYYG+D D+ 
Sbjct: 148  NTFADIRRDETHRLIRKLAEDASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMA 207

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +++EA +FR M++E+L+L+G NNK DF+PLL   D+ENLE +LKKIS K D FL GLL+E
Sbjct: 208  DVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDYENLEKRLKKISSKTDTFLNGLLEE 267

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
            QR KK+R NTM+DHLLSLQESQP+YYTD+IIKGLAL ML+AGTDSSAVTL+W+LS +L +
Sbjct: 268  QRSKKQRANTMVDHLLSLQESQPDYYTDEIIKGLALGMLVAGTDSSAVTLEWALSCVLKH 327

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PEVLK+ RDEL+T VG +RL+EESD+ KL YLKN+I ET            HS+S+ECII
Sbjct: 328  PEVLKRTRDELETQVGQERLLEESDISKLPYLKNIIFETLRLYTPAPLLLPHSSSEECII 387

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GG+KVPRD+IV++NAW+I RDP++WSEAT+FKPER+E EG
Sbjct: 388  GGFKVPRDSIVLINAWSIHRDPQVWSEATSFKPERYESEG 427


>ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
 gb|ESW30851.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
          Length = 500

 Score =  558 bits (1437), Expect = 0.0
 Identities = 267/400 (66%), Positives = 326/400 (81%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L+ Q+R F+              LHHLKRPL   FK LS+ YG ++SLWFGSRLVV+VSS
Sbjct: 24   LLLQARNFQNLPPGPPSLPIIGNLHHLKRPLHRHFKALSDNYGHVISLWFGSRLVVVVSS 83

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             ++FQ CFTK+D+ LANRP+FLSGKYIFYNYTTLGS  YG  WRNLRRITAL++LS QRI
Sbjct: 84   HSLFQHCFTKNDIALANRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTQRI 143

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            N+F+ IR+DET RLI KLAE +S+DF EVEL+S+FYDMTFNNIMRMISGKRYYG+D D+ 
Sbjct: 144  NTFAAIRRDETHRLIRKLAEGASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMA 203

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +++EA +FR M++E+L+L+G NNK DF+PLL   DFENLE +LKKIS K D FL GLL+E
Sbjct: 204  DVEEARQFRAMVSELLKLSGANNKNDFMPLLRLFDFENLEKRLKKISSKTDTFLNGLLEE 263

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
             R KK+R NTM+DHLLSLQESQPEYYTDQIIKGLAL ML+AGTDSSAVTL+W+LS ++N+
Sbjct: 264  HRSKKQRANTMVDHLLSLQESQPEYYTDQIIKGLALGMLIAGTDSSAVTLEWALSCVVNH 323

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PEV+K+ RDEL+THVG +RL++ESDL KL YLKN+I ET            HS+S+EC I
Sbjct: 324  PEVMKRARDELETHVGEERLLDESDLSKLPYLKNIIFETLRLYTPAPLLLPHSSSEECNI 383

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            GG+KVPRD+IV++NAW+I RDPE+WSEAT+FKPERFE EG
Sbjct: 384  GGFKVPRDSIVLINAWSIHRDPEVWSEATSFKPERFENEG 423


>gb|KHN26592.1| Isoflavone 2'-hydroxylase [Glycine soja]
          Length = 499

 Score =  502 bits (1292), Expect = e-171
 Identities = 250/400 (62%), Positives = 303/400 (75%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L  Q RK K              L+ L+RPL       S+ YGDI SLWFGSRL V+VSS
Sbjct: 22   LFSQRRKHKNLPPGPPPLPIIGNLNLLERPLHRFLTRTSKTYGDIFSLWFGSRLAVVVSS 81

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             + FQECFT++DV LANRP+ LSGK+IFYNYTT+GS SYGE WRNLRRIT+LDVLS QRI
Sbjct: 82   PSAFQECFTRNDVALANRPRSLSGKHIFYNYTTVGSCSYGEHWRNLRRITSLDVLSTQRI 141

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            +SFSGIRKDET+R I  LA+DS  D+  VELSS F+DMT+NN+MRM+SGKRYYG++  + 
Sbjct: 142  HSFSGIRKDETERFIRMLAKDSCMDYAHVELSSMFHDMTYNNMMRMLSGKRYYGDESQIK 201

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +++EA  FR+ + E+LQ+ G +NK D++P L + DF NLE +LK I+++ D FL  L+ E
Sbjct: 202  DVEEAKEFRETVEELLQVAGVSNKADYLPFLRWFDFHNLEKRLKSINKRFDTFLDKLIHE 261

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
            QR KKER NTMIDHLL LQESQPEYYTDQIIKGL LAML AGTDSSAVTL+WSLS+LLN+
Sbjct: 262  QRSKKERENTMIDHLLHLQESQPEYYTDQIIKGLVLAMLFAGTDSSAVTLEWSLSNLLNH 321

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PEVLKK RDELDT+VG DRLV ESDLP L YLK +I ET            H +S++  I
Sbjct: 322  PEVLKKARDELDTYVGQDRLVNESDLPNLFYLKKIILETLRLHPPAPLAIPHVSSEDITI 381

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            G + VPRDTIVM+N WA+QRDP +W+EAT FKPERF++EG
Sbjct: 382  GEFNVPRDTIVMINIWAMQRDPLVWNEATCFKPERFDEEG 421


>ref|XP_003533544.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
 gb|KRH37168.1| hypothetical protein GLYMA_09G048700 [Glycine max]
          Length = 501

 Score =  502 bits (1292), Expect = e-171
 Identities = 250/400 (62%), Positives = 303/400 (75%)
 Frame = +3

Query: 309  LIFQSRKFKXXXXXXXXXXXXXXLHHLKRPLQHTFKGLSEKYGDIMSLWFGSRLVVIVSS 488
            L  Q RK K              L+ L+RPL       S+ YGDI SLWFGSRL V+VSS
Sbjct: 24   LFSQRRKHKNLPPGPPPLPIIGNLNLLERPLHRFLTRTSKTYGDIFSLWFGSRLAVVVSS 83

Query: 489  QAIFQECFTKHDVVLANRPKFLSGKYIFYNYTTLGSVSYGELWRNLRRITALDVLSNQRI 668
             + FQECFT++DV LANRP+ LSGK+IFYNYTT+GS SYGE WRNLRRIT+LDVLS QRI
Sbjct: 84   PSAFQECFTRNDVALANRPRSLSGKHIFYNYTTVGSCSYGEHWRNLRRITSLDVLSTQRI 143

Query: 669  NSFSGIRKDETQRLITKLAEDSSTDFVEVELSSRFYDMTFNNIMRMISGKRYYGEDLDVL 848
            +SFSGIRKDET+R I  LA+DS  D+  VELSS F+DMT+NN+MRM+SGKRYYG++  + 
Sbjct: 144  HSFSGIRKDETERFIRMLAKDSCMDYAHVELSSMFHDMTYNNMMRMLSGKRYYGDESQIK 203

Query: 849  ELQEASRFRDMIAEVLQLTGPNNKTDFIPLLGFLDFENLETKLKKISEKNDAFLRGLLQE 1028
            +++EA  FR+ + E+LQ+ G +NK D++P L + DF NLE +LK I+++ D FL  L+ E
Sbjct: 204  DVEEAKEFRETVEELLQVAGVSNKADYLPFLRWFDFHNLEKRLKSINKRFDTFLDKLIHE 263

Query: 1029 QRDKKERTNTMIDHLLSLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLDWSLSSLLNN 1208
            QR KKER NTMIDHLL LQESQPEYYTDQIIKGL LAML AGTDSSAVTL+WSLS+LLN+
Sbjct: 264  QRSKKERENTMIDHLLHLQESQPEYYTDQIIKGLVLAMLFAGTDSSAVTLEWSLSNLLNH 323

Query: 1209 PEVLKKVRDELDTHVGPDRLVEESDLPKLTYLKNVINETXXXXXXXXXXXXHSTSDECII 1388
            PEVLKK RDELDT+VG DRLV ESDLP L YLK +I ET            H +S++  I
Sbjct: 324  PEVLKKARDELDTYVGQDRLVNESDLPNLFYLKKIILETLRLHPPAPLAIPHVSSEDITI 383

Query: 1389 GGYKVPRDTIVMVNAWAIQRDPELWSEATTFKPERFEKEG 1508
            G + VPRDTIVM+N WA+QRDP +W+EAT FKPERF++EG
Sbjct: 384  GEFNVPRDTIVMINIWAMQRDPLVWNEATCFKPERFDEEG 423