BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00013127
         (1819 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003552078.1| PREDICTED: mitochondrial ubiquitin ligase ac...   545   0.0  
ref|XP_007135436.1| hypothetical protein PHAVU_010G129300g [Phas...   544   0.0  
ref|XP_003520919.1| PREDICTED: mitochondrial ubiquitin ligase ac...   538   0.0  
ref|XP_017442424.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 ...   538   0.0  
ref|XP_020232197.1| E3 ubiquitin-protein ligase SPL2 [Cajanus ca...   534   0.0  
ref|XP_006576561.1| PREDICTED: mitochondrial ubiquitin ligase ac...   531   0.0  
ref|XP_003627295.1| E3 ubiquitin ligase [Medicago truncatula] >g...   521   e-179
ref|XP_019425633.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 ...   519   e-178
ref|XP_014515114.1| E3 ubiquitin-protein ligase SPL2 [Vigna radi...   517   e-177
ref|XP_012574098.1| PREDICTED: mitochondrial ubiquitin ligase ac...   514   e-176
ref|XP_016176316.1| E3 ubiquitin-protein ligase SPL2 [Arachis ip...   514   e-176
ref|XP_015939227.1| E3 ubiquitin-protein ligase SPL2 [Arachis du...   511   e-175
ref|XP_003547746.1| PREDICTED: mitochondrial ubiquitin ligase ac...   509   e-174
ref|XP_002274016.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 ...   476   e-161
gb|KHN39625.1| Mitochondrial ubiquitin ligase activator of nfkb ...   469   e-160
ref|XP_021628506.1| E3 ubiquitin-protein ligase SPL2 isoform X2 ...   470   e-159
ref|XP_021628505.1| E3 ubiquitin-protein ligase SPL2 isoform X1 ...   470   e-159
ref|XP_012068880.1| E3 ubiquitin-protein ligase SPL2 [Jatropha c...   466   e-157
ref|XP_010086805.1| E3 ubiquitin-protein ligase SPL2 [Morus nota...   466   e-157
gb|PON88937.1| Mitochondrial ubiquitin ligase activator of NFKB ...   462   e-156

>ref|XP_003552078.1| PREDICTED: mitochondrial ubiquitin ligase activator of NFKB 1-like
            [Glycine max]
 gb|KRG99500.1| hypothetical protein GLYMA_18G149800 [Glycine max]
          Length = 387

 Score =  545 bits (1403), Expect = 0.0
 Identities = 272/357 (76%), Positives = 300/357 (84%), Gaps = 1/357 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            VR++ KF+A ++A+RKL  APSLSVSDLRSLLA+  SD D NS  GIIVIVRGTVDAKSA
Sbjct: 33   VRTLFKFTATSSALRKLRRAPSLSVSDLRSLLAEIPSDGDGNSKGGIIVIVRGTVDAKSA 92

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+G+WKTLRP VLVS ESGDKGVILQRTQTCIYNEW GLFGW+SDLR IF R+WRQQEST
Sbjct: 93   VDGTWKTLRPGVLVSRESGDKGVILQRTQTCIYNEWKGLFGWTSDLRAIFARSWRQQEST 152

Query: 548  SLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPIT-TPYTFLQAFFWRQY 724
            SLRKVPF+LTD GRR N EYVVVN+DGSRHPLPLTTVYHKL+PIT +PYTFLQA F  +Y
Sbjct: 153  SLRKVPFLLTDVGRRPNAEYVVVNMDGSRHPLPLTTVYHKLQPITASPYTFLQALFGHEY 212

Query: 725  PIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTKT 904
            P+GLLDEEKILPLGKDI+AVGLC L++GIAEIKSCK LPYFLS+LSKDQMI+DLS KTK 
Sbjct: 213  PVGLLDEEKILPLGKDITAVGLCSLKNGIAEIKSCKYLPYFLSDLSKDQMIMDLSIKTKI 272

Query: 905  LFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDVP 1084
            LFW GI LGS+SVG+LGYA+V                         DV+PQ DDEIED P
Sbjct: 273  LFWGGIALGSMSVGVLGYAVVRNWNKWKQWKQQRQLQQQRQAV--SDVEPQMDDEIEDAP 330

Query: 1085 DGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DGQLCVICLMRRRRSVFIPCGHLVCC GCAISVE EVAPKCPVCRQEIR+SVRI+ES
Sbjct: 331  DGQLCVICLMRRRRSVFIPCGHLVCCQGCAISVEREVAPKCPVCRQEIRDSVRIYES 387


>ref|XP_007135436.1| hypothetical protein PHAVU_010G129300g [Phaseolus vulgaris]
 gb|ESW07430.1| hypothetical protein PHAVU_010G129300g [Phaseolus vulgaris]
          Length = 387

 Score =  544 bits (1402), Expect = 0.0
 Identities = 272/357 (76%), Positives = 299/357 (83%), Gaps = 1/357 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            +R+++KFSA + A+RKL  AP LSVSDLRSLLADT SDA+ NSD G IVIVRG VDAKSA
Sbjct: 33   IRTLVKFSATSAALRKLRRAPYLSVSDLRSLLADTPSDANSNSDGGAIVIVRGNVDAKSA 92

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+GSWKTLRP VLVS ESGD+GVILQRTQTCIYNEW GLFGW+SDLR IF R+WRQQEST
Sbjct: 93   VDGSWKTLRPGVLVSRESGDRGVILQRTQTCIYNEWKGLFGWTSDLRAIFARSWRQQEST 152

Query: 548  SLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQAFFWRQY 724
            SLRKVPFVL D G R +TEYVVVN+DGSRHPLPLTTVYHKL+PI  +PYTFLQA F  +Y
Sbjct: 153  SLRKVPFVLIDVGWRPSTEYVVVNMDGSRHPLPLTTVYHKLQPINASPYTFLQALFGHEY 212

Query: 725  PIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTKT 904
            P+GLLDEEKILP+GKD++AVGL  L +G+AEIKSCKDLPYFLS+LSKDQMIVDLS KTK 
Sbjct: 213  PVGLLDEEKILPVGKDVTAVGLVSLNNGVAEIKSCKDLPYFLSDLSKDQMIVDLSIKTKI 272

Query: 905  LFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDVP 1084
            LFW GI LGS+SVGILGYA+V                         DV+PQ DDEIEDVP
Sbjct: 273  LFWGGIALGSMSVGILGYAVVRNWNKWKQWKLQRQLQQQRQAV--SDVEPQVDDEIEDVP 330

Query: 1085 DGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DGQLCVICLMRRRRSVFIPCGHLVCC GCAISVE+EVAPKCPVCRQEIRNSVRIFES
Sbjct: 331  DGQLCVICLMRRRRSVFIPCGHLVCCQGCAISVEHEVAPKCPVCRQEIRNSVRIFES 387


>ref|XP_003520919.1| PREDICTED: mitochondrial ubiquitin ligase activator of NFKB 1-like
            isoform X2 [Glycine max]
 gb|KRH66057.1| hypothetical protein GLYMA_03G080300 [Glycine max]
          Length = 388

 Score =  538 bits (1386), Expect = 0.0
 Identities = 272/358 (75%), Positives = 299/358 (83%), Gaps = 2/358 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            VR++ KF+A + A+RKLH APS+SVSDLRSLLA+  SDAD NSD G IVIVRGTVDAKS 
Sbjct: 33   VRTLFKFTATSAALRKLHRAPSVSVSDLRSLLAEIPSDADGNSDGGNIVIVRGTVDAKST 92

Query: 368  VEG-SWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQES 544
            VEG +WKTLRP VLVS ESGDK VILQRTQTCIYNEW GLFGW+SDLR IF R+WRQQES
Sbjct: 93   VEGGTWKTLRPGVLVSRESGDKSVILQRTQTCIYNEWKGLFGWTSDLRAIFARSWRQQES 152

Query: 545  TSLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPIT-TPYTFLQAFFWRQ 721
            TSLRKVPFVL D GR  N EYVVVN+DGSRHPLPL+TVYHKL+PIT +PYTFLQA F  +
Sbjct: 153  TSLRKVPFVLIDVGRWPNAEYVVVNMDGSRHPLPLSTVYHKLQPITASPYTFLQALFGHE 212

Query: 722  YPIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTK 901
            YP+GLLDEEKILPLGK+I+AVGLC L++GIAEIKSCKDLPYFLS+LSKDQMIVDLS KTK
Sbjct: 213  YPVGLLDEEKILPLGKNITAVGLCSLKNGIAEIKSCKDLPYFLSDLSKDQMIVDLSSKTK 272

Query: 902  TLFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDV 1081
             LFW GI LGS+SVG+LGYA+V                         DV+PQ DDEIEDV
Sbjct: 273  ILFWGGIALGSMSVGVLGYAVVRNWNKWKQWKQQRQLQQQRQAV--SDVEPQMDDEIEDV 330

Query: 1082 PDGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            PDGQLCVICLMRRRRSVFIPCGHLVCC GCAISVE EVAPKCPVCRQEIR+SVRI+ES
Sbjct: 331  PDGQLCVICLMRRRRSVFIPCGHLVCCQGCAISVEREVAPKCPVCRQEIRDSVRIYES 388


>ref|XP_017442424.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 [Vigna angularis]
 gb|KOM57062.1| hypothetical protein LR48_Vigan11g009400 [Vigna angularis]
 dbj|BAT98292.1| hypothetical protein VIGAN_09193700 [Vigna angularis var. angularis]
          Length = 388

 Score =  538 bits (1385), Expect = 0.0
 Identities = 271/358 (75%), Positives = 298/358 (83%), Gaps = 2/358 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            +R++ KFSA + A+RKL  APSLSVSDLRSLLADT SDAD  SD G IVIVRGTV+AKSA
Sbjct: 33   IRTLFKFSATSAALRKLRRAPSLSVSDLRSLLADTPSDADSTSDGGTIVIVRGTVNAKSA 92

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+GSWKTLRP VLVS ESGDKGVILQRTQTCIYNEW GLFGW+SDLR +F R+WRQQEST
Sbjct: 93   VDGSWKTLRPGVLVSRESGDKGVILQRTQTCIYNEWKGLFGWTSDLRALFARSWRQQEST 152

Query: 548  SLRKVPFVLTDDGRRSNT-EYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQAFFWRQ 721
            SLRKVPFVL D GR+ +T EYVVVN+DGSRHPLPLTTVYHKL+PI  +PYTFLQA F  +
Sbjct: 153  SLRKVPFVLIDVGRQPSTPEYVVVNMDGSRHPLPLTTVYHKLQPINASPYTFLQALFGHE 212

Query: 722  YPIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTK 901
            YP+GLLDEEKILPLGKDI+AVGL  L +G+AEIKSCKDLPYFLS+LSKDQMIVDLS KTK
Sbjct: 213  YPVGLLDEEKILPLGKDITAVGLGSLNNGVAEIKSCKDLPYFLSDLSKDQMIVDLSIKTK 272

Query: 902  TLFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDV 1081
             LFW GI LGS+S+GILGYA+V                         +V+PQ DDEIEDV
Sbjct: 273  ILFWGGITLGSMSIGILGYAVVRNWNKWKQWKLQRQLQQQRQAV--NNVEPQVDDEIEDV 330

Query: 1082 PDGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            PDGQLCVICLMRRRRS FIPCGHLVCC GCAISVE EVAPKCPVCRQEIRNSVRIFE+
Sbjct: 331  PDGQLCVICLMRRRRSAFIPCGHLVCCQGCAISVEREVAPKCPVCRQEIRNSVRIFET 388


>ref|XP_020232197.1| E3 ubiquitin-protein ligase SPL2 [Cajanus cajan]
 gb|KYP50495.1| hypothetical protein KK1_027747 [Cajanus cajan]
          Length = 381

 Score =  534 bits (1376), Expect = 0.0
 Identities = 270/357 (75%), Positives = 297/357 (83%), Gaps = 1/357 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            VR++ KF+A +TA+RKL  APSLSVSDLRSLLAD+      NS DG IVI RGTVDAKSA
Sbjct: 33   VRTLFKFTATSTALRKLRHAPSLSVSDLRSLLADS------NSHDGQIVIARGTVDAKSA 86

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+GSWKTLRP VLVS ESGDKGVILQRTQTCIYNEW GLFGW+SDLR IF R+WRQQEST
Sbjct: 87   VDGSWKTLRPGVLVSRESGDKGVILQRTQTCIYNEWKGLFGWTSDLRAIFARSWRQQEST 146

Query: 548  SLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQAFFWRQY 724
            SLRKVPFVL D G+R N+EYVVVN+DGSRHPLPLTTVYHKL+PI  +PYTFLQA F  +Y
Sbjct: 147  SLRKVPFVLIDIGQRPNSEYVVVNMDGSRHPLPLTTVYHKLQPINASPYTFLQALFGHEY 206

Query: 725  PIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTKT 904
            P+GLLDEEKILPLGKDI+AVG+C L++GIAEIKSCKDLPYFLS+LSKDQMIVDLS KTK 
Sbjct: 207  PVGLLDEEKILPLGKDITAVGVCSLKNGIAEIKSCKDLPYFLSDLSKDQMIVDLSIKTKV 266

Query: 905  LFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDVP 1084
            LFW GI LGS+SVGIL YA+V                         DV+PQ D+EIEDVP
Sbjct: 267  LFWGGIALGSMSVGILSYAVVRNWNKWKQWKQHRQLQQQRQAV--RDVEPQVDEEIEDVP 324

Query: 1085 DGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DGQLCVICLMRRRRSVFIPCGHLVCC GCAISVE EVAPKCPVCRQEIR+SVRI+ES
Sbjct: 325  DGQLCVICLMRRRRSVFIPCGHLVCCQGCAISVEREVAPKCPVCRQEIRDSVRIYES 381


>ref|XP_006576561.1| PREDICTED: mitochondrial ubiquitin ligase activator of NFKB 1-like
            isoform X1 [Glycine max]
          Length = 394

 Score =  531 bits (1369), Expect = 0.0
 Identities = 272/364 (74%), Positives = 299/364 (82%), Gaps = 8/364 (2%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            VR++ KF+A + A+RKLH APS+SVSDLRSLLA+  SDAD NSD G IVIVRGTVDAKS 
Sbjct: 33   VRTLFKFTATSAALRKLHRAPSVSVSDLRSLLAEIPSDADGNSDGGNIVIVRGTVDAKST 92

Query: 368  VEG-SWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQES 544
            VEG +WKTLRP VLVS ESGDK VILQRTQTCIYNEW GLFGW+SDLR IF R+WRQQES
Sbjct: 93   VEGGTWKTLRPGVLVSRESGDKSVILQRTQTCIYNEWKGLFGWTSDLRAIFARSWRQQES 152

Query: 545  TSLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPIT-TPYTFLQAFFWRQ 721
            TSLRKVPFVL D GR  N EYVVVN+DGSRHPLPL+TVYHKL+PIT +PYTFLQA F  +
Sbjct: 153  TSLRKVPFVLIDVGRWPNAEYVVVNMDGSRHPLPLSTVYHKLQPITASPYTFLQALFGHE 212

Query: 722  YPIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFL------SELSKDQMIVD 883
            YP+GLLDEEKILPLGK+I+AVGLC L++GIAEIKSCKDLPYFL      S+LSKDQMIVD
Sbjct: 213  YPVGLLDEEKILPLGKNITAVGLCSLKNGIAEIKSCKDLPYFLFLYCCRSDLSKDQMIVD 272

Query: 884  LSFKTKTLFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPD 1063
            LS KTK LFW GI LGS+SVG+LGYA+V                         DV+PQ D
Sbjct: 273  LSSKTKILFWGGIALGSMSVGVLGYAVVRNWNKWKQWKQQRQLQQQRQAV--SDVEPQMD 330

Query: 1064 DEIEDVPDGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVR 1243
            DEIEDVPDGQLCVICLMRRRRSVFIPCGHLVCC GCAISVE EVAPKCPVCRQEIR+SVR
Sbjct: 331  DEIEDVPDGQLCVICLMRRRRSVFIPCGHLVCCQGCAISVEREVAPKCPVCRQEIRDSVR 390

Query: 1244 IFES 1255
            I+ES
Sbjct: 391  IYES 394


>ref|XP_003627295.1| E3 ubiquitin ligase [Medicago truncatula]
 emb|CAJ00009.1| C3HC4 zinc finger containing protein [Medicago truncatula]
 gb|AET01771.1| E3 ubiquitin ligase [Medicago truncatula]
          Length = 383

 Score =  521 bits (1342), Expect = e-179
 Identities = 262/357 (73%), Positives = 294/357 (82%), Gaps = 1/357 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            VRSI KF+  + A+RK+  APS+SVSDLRSLL +T    DENSD+G IVIVRGTVDAK+ 
Sbjct: 33   VRSIRKFTLTSAALRKITAAPSVSVSDLRSLLTET----DENSDEGKIVIVRGTVDAKNV 88

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+GSWK L P VLVS ESGDKGV+LQRTQTCIYNEW GLFGW+SDLR +F R+WRQQEST
Sbjct: 89   VDGSWKALWPGVLVSSESGDKGVVLQRTQTCIYNEWKGLFGWTSDLRALFLRSWRQQEST 148

Query: 548  SLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPIT-TPYTFLQAFFWRQY 724
            SLRK+PFVL D GR SN EYVVVN+DGS HPLPLTTVYH+L+P+   PYTFLQA F  +Y
Sbjct: 149  SLRKIPFVLIDVGRPSNPEYVVVNMDGSSHPLPLTTVYHRLQPVNPPPYTFLQALFGHEY 208

Query: 725  PIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTKT 904
            P+GLLDEEKILPLGKD+SAVGLC LR+GIAEIK+C DLPY+LS+LSKDQMIVDLSFKTK 
Sbjct: 209  PVGLLDEEKILPLGKDVSAVGLCSLRNGIAEIKACNDLPYYLSDLSKDQMIVDLSFKTKL 268

Query: 905  LFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDVP 1084
            LFWSGILLGS+SVGI+GYA+V                      I  +  P  DDEIEDVP
Sbjct: 269  LFWSGILLGSMSVGIIGYAVVRNWNKWKQWKQQRQLQQRRQQPI--EPVPPTDDEIEDVP 326

Query: 1085 DGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DGQLCVICLMRRRRSVFIPCGHLVCC GCAISVE+EVAPKCPVCRQE+R+SVRIFES
Sbjct: 327  DGQLCVICLMRRRRSVFIPCGHLVCCQGCAISVESEVAPKCPVCRQEVRDSVRIFES 383


>ref|XP_019425633.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 [Lupinus angustifolius]
          Length = 387

 Score =  519 bits (1337), Expect = e-178
 Identities = 263/357 (73%), Positives = 292/357 (81%), Gaps = 1/357 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            VRS++ F+A + A+RKL  APSLSVSDLRS+L ++ SD D NS DG IVIVRGTVDAKSA
Sbjct: 33   VRSLLNFTATSAALRKLRTAPSLSVSDLRSILTES-SDEDGNSGDGKIVIVRGTVDAKSA 91

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+GSWK L P VLVS ESGDK V++QRTQTCIYNEW GLFGW+SDLR IF R+WR+QEST
Sbjct: 92   VDGSWKALMPGVLVSRESGDKAVVIQRTQTCIYNEWKGLFGWTSDLRAIFTRSWRKQEST 151

Query: 548  SLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQAFFWRQY 724
            SLRKVPFVL D GRR N+ YVVVNLDGSRHPLPLTTVYHKL+PI  +PYTFLQA F  +Y
Sbjct: 152  SLRKVPFVLIDVGRRPNSGYVVVNLDGSRHPLPLTTVYHKLQPINASPYTFLQALFGHEY 211

Query: 725  PIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTKT 904
            P+GLLDEEKILPLGKDISAVGLC LR+GIAEIK  KDLPYFLS+LSKDQMIVDLSFKT  
Sbjct: 212  PVGLLDEEKILPLGKDISAVGLCSLRNGIAEIKPSKDLPYFLSDLSKDQMIVDLSFKTTV 271

Query: 905  LFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDVP 1084
            L W GI+LGS+SVGILGYA+V                      +SGD   Q +DE EDVP
Sbjct: 272  LLWGGIVLGSMSVGILGYAVV-RNWNKWKQWKQQRELQQQRQDVSGDFVYQLEDENEDVP 330

Query: 1085 DGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DGQLCV+CLMRRRRS FIPCGHLVCCH CA+SVE EVAPKCPVCRQEIR+SVRIFES
Sbjct: 331  DGQLCVVCLMRRRRSAFIPCGHLVCCHRCAVSVEREVAPKCPVCRQEIRDSVRIFES 387


>ref|XP_014515114.1| E3 ubiquitin-protein ligase SPL2 [Vigna radiata var. radiata]
          Length = 389

 Score =  517 bits (1332), Expect = e-177
 Identities = 263/359 (73%), Positives = 290/359 (80%), Gaps = 3/359 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            +R+  KFSA + A+RKL  AP LSVSDLRSLLAD+  DAD  SD G IVIVRGTVDAKSA
Sbjct: 33   IRTFFKFSATSAALRKLRRAPYLSVSDLRSLLADSPPDADSTSDGGTIVIVRGTVDAKSA 92

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+GSWKTLRP VLVS ESGDKGVILQRTQTCIYNEW GL GW+SDLR    R+WR+QEST
Sbjct: 93   VDGSWKTLRPGVLVSRESGDKGVILQRTQTCIYNEWKGLLGWTSDLRAFIARSWRKQEST 152

Query: 548  SLRKVPFVLTDDGRRSNT-EYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQAFFWRQ 721
            SLRKVPFVL D GRR +T EYVVVN+DGSRHPLPLTTVYHKL+PI  +PYTFLQA F  +
Sbjct: 153  SLRKVPFVLIDVGRRPSTPEYVVVNMDGSRHPLPLTTVYHKLQPINASPYTFLQALFGHE 212

Query: 722  YPIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTK 901
            YP+GLLDEEKILPLGKDI+ VGL  L +G+AEIKSCKDLPYFLS+LSKDQMIVDLS KTK
Sbjct: 213  YPVGLLDEEKILPLGKDITVVGLGSLNNGVAEIKSCKDLPYFLSDLSKDQMIVDLSIKTK 272

Query: 902  TLFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQ-PDDEIED 1078
             LFW GI +GS+SVGILGYA+V                         +V+PQ  DDEIED
Sbjct: 273  ILFWGGIAIGSMSVGILGYAVVRNWNKWKQWKLQRQLQQQRQAV--NNVEPQIDDDEIED 330

Query: 1079 VPDGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            VPDGQLCVIC+MRRRRS FIPCGHLVCC GCAI+VE EV PKCPVCRQEIRNSVRIFE+
Sbjct: 331  VPDGQLCVICVMRRRRSAFIPCGHLVCCQGCAIAVEREVLPKCPVCRQEIRNSVRIFET 389


>ref|XP_012574098.1| PREDICTED: mitochondrial ubiquitin ligase activator of nfkb 1 [Cicer
            arietinum]
          Length = 387

 Score =  514 bits (1324), Expect = e-176
 Identities = 254/357 (71%), Positives = 296/357 (82%), Gaps = 1/357 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            VRSI +F   + AIRK++ APSLSVSDLRS+L +  SD D NSD G +VIVRGTVDAKS+
Sbjct: 33   VRSIRRFFTTSAAIRKINRAPSLSVSDLRSILDENDSDGDGNSDYGKVVIVRGTVDAKSS 92

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+GSWKTL P VLVS +SGDKGVI+QRTQTCIY+EW G+FGW+SD+R +F R+WR++EST
Sbjct: 93   VDGSWKTLWPGVLVSRDSGDKGVIIQRTQTCIYSEWRGIFGWTSDIRALFTRSWRRREST 152

Query: 548  SLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPITTP-YTFLQAFFWRQY 724
            SLRKVPFVL D GRRS+ EYVVV++DGS+HPLPLTTVYHK++PI  P YTFLQA F  +Y
Sbjct: 153  SLRKVPFVLIDVGRRSSAEYVVVSMDGSKHPLPLTTVYHKMQPINPPVYTFLQALFGHEY 212

Query: 725  PIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTKT 904
            P+GLLDEEKILPLGKDISAVGLC LR+GIAEIKSC DLPYFLS+LSK+QMI++LSFK K 
Sbjct: 213  PVGLLDEEKILPLGKDISAVGLCSLRNGIAEIKSCNDLPYFLSDLSKEQMILELSFKMKI 272

Query: 905  LFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDVP 1084
            LFWSGI+LGS+SVGIL YA+V                      +  +V+ Q DDEIEDVP
Sbjct: 273  LFWSGIVLGSMSVGILSYAVVKNWNRWKQWKQRRQLQQQRQTIV--EVNSQQDDEIEDVP 330

Query: 1085 DGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DGQLCVICLMRRRRSVFIPCGHLVCC GCA+SVE+EVAPKCPVCRQE+ +SVRIFES
Sbjct: 331  DGQLCVICLMRRRRSVFIPCGHLVCCQGCAVSVESEVAPKCPVCRQEVHDSVRIFES 387


>ref|XP_016176316.1| E3 ubiquitin-protein ligase SPL2 [Arachis ipaensis]
          Length = 384

 Score =  514 bits (1323), Expect = e-176
 Identities = 256/357 (71%), Positives = 292/357 (81%), Gaps = 1/357 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            +RSI  F+ N+ ++RKL  APS+SVSDLRSLL    SD+D NSDDG IV+VRGTV+ K+A
Sbjct: 33   IRSIRNFTTNSASLRKLRRAPSVSVSDLRSLL----SDSDGNSDDGGIVVVRGTVEPKAA 88

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+GSWK+LRPS+LVS ESGDK V+LQ TQTCIYNEW GLFGW+SDLR IF R+WRQ ES 
Sbjct: 89   VDGSWKSLRPSLLVSRESGDKAVVLQITQTCIYNEWKGLFGWTSDLRAIFARSWRQPESK 148

Query: 548  SLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQAFFWRQY 724
             LRKVPFVL D GR+ +TEYVVVNLDGSRHPLPLTTVYHKL+PI  +PYTFLQA F  +Y
Sbjct: 149  FLRKVPFVLVDVGRQPSTEYVVVNLDGSRHPLPLTTVYHKLQPINASPYTFLQALFGHEY 208

Query: 725  PIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTKT 904
            P+GLL+EEKILP+GKDI+AVGLC +R+GI EIKSCKDLPYFLSELSKDQMIVDLSFKTK 
Sbjct: 209  PVGLLEEEKILPVGKDITAVGLCSVRNGIPEIKSCKDLPYFLSELSKDQMIVDLSFKTKV 268

Query: 905  LFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDVP 1084
            LFW GI+LGSVS+GILGY++                       I  +VDPQ D++ ED+ 
Sbjct: 269  LFWGGIVLGSVSIGILGYSVARNWNKWMQWKEQRQRQQQRQAVID-EVDPQVDEQNEDIA 327

Query: 1085 DGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DGQLCVICLMRRRRSVF+PCGHLVCC GCAISVE EVAPKCPVCRQEIR SVRIFES
Sbjct: 328  DGQLCVICLMRRRRSVFVPCGHLVCCQGCAISVEREVAPKCPVCRQEIRQSVRIFES 384


>ref|XP_015939227.1| E3 ubiquitin-protein ligase SPL2 [Arachis duranensis]
          Length = 384

 Score =  511 bits (1315), Expect = e-175
 Identities = 255/357 (71%), Positives = 291/357 (81%), Gaps = 1/357 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            +RSI  F+ N+ ++RKL  APS+SVSDLRSL    +SD+D NSDDG IV+VRGTV+ K+A
Sbjct: 33   IRSIRNFTTNSASLRKLRRAPSVSVSDLRSL----ISDSDGNSDDGRIVVVRGTVEPKAA 88

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+GSWK+LRPS+LVS ES DK V+LQ TQTCIYNEW GLFGW+SDLR IF R+WRQ ES 
Sbjct: 89   VDGSWKSLRPSLLVSRESCDKAVVLQITQTCIYNEWKGLFGWTSDLRAIFARSWRQPESK 148

Query: 548  SLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQAFFWRQY 724
             LRKVPFVL D GR+ +TEYVVVNLDGSRHPLPLTTVYHKL+PI  +PYTFLQA F  +Y
Sbjct: 149  FLRKVPFVLVDVGRQPSTEYVVVNLDGSRHPLPLTTVYHKLQPINASPYTFLQALFGHEY 208

Query: 725  PIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTKT 904
            P+GLL+EEKILP+GKDI+AVGLC +R+GI EIKSCKDLPYFLSELSKDQMIVDLSFKTK 
Sbjct: 209  PVGLLEEEKILPVGKDITAVGLCSVRNGIPEIKSCKDLPYFLSELSKDQMIVDLSFKTKV 268

Query: 905  LFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDVP 1084
            LFW GI+LGSVS+GILGY++                       I  +VDPQ D++ ED+ 
Sbjct: 269  LFWGGIVLGSVSIGILGYSVARNWNKWMQWKEQRQRQQQRQAVID-EVDPQVDEQNEDIA 327

Query: 1085 DGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DGQLCVICLMRRRRSVFIPCGHLVCC GCAISVE EVAPKCPVCRQEIR SVRIFES
Sbjct: 328  DGQLCVICLMRRRRSVFIPCGHLVCCQGCAISVEREVAPKCPVCRQEIRESVRIFES 384


>ref|XP_003547746.1| PREDICTED: mitochondrial ubiquitin ligase activator of NFKB 1-like
            [Glycine max]
 gb|KRH06193.1| hypothetical protein GLYMA_16G008300 [Glycine max]
          Length = 383

 Score =  509 bits (1312), Expect = e-174
 Identities = 256/357 (71%), Positives = 289/357 (80%), Gaps = 1/357 (0%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGIIVIVRGTVDAKSA 367
            VR++ KF+A + ++RKL  APS+SVSDLRSLLA+  SDAD     G IVI+RGTVDAKSA
Sbjct: 33   VRTLFKFTATSASLRKLRRAPSISVSDLRSLLAEPPSDADA----GEIVIIRGTVDAKSA 88

Query: 368  VEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQEST 547
            V+G+WKTL+P VLVS ESGDKGVILQRTQTCIYN+W GL GW+SDLR I  R+ RQQ+ST
Sbjct: 89   VDGTWKTLKPGVLVSRESGDKGVILQRTQTCIYNDWKGLLGWTSDLRAICARSLRQQDST 148

Query: 548  SLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQAFFWRQY 724
            S RKVPFVL D GRR N EYVVVN+DGSRHPLPLTTVYHKL+PI  +PYTFLQA F  +Y
Sbjct: 149  SFRKVPFVLIDVGRRPNAEYVVVNMDGSRHPLPLTTVYHKLQPINASPYTFLQALFGHEY 208

Query: 725  PIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKTKT 904
            P+GLLDEEKILPLGKDI+AVG C L++GIAEIKSCKD+PYFLS+LSKDQMIVDLS K K 
Sbjct: 209  PVGLLDEEKILPLGKDITAVGHCSLKNGIAEIKSCKDIPYFLSDLSKDQMIVDLSIKAKI 268

Query: 905  LFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIEDVP 1084
            LFW GI LGS+SVG+LGYA++                         D +PQ DDEIEDVP
Sbjct: 269  LFWGGISLGSMSVGVLGYAVLRNWIKWKRWKVQRQLQQQRQAV--SDAEPQVDDEIEDVP 326

Query: 1085 DGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DGQLCVICLMRRRRSVFIPCGHLVCC GCAISVE EVAPKCPVCRQEIR+SVR +ES
Sbjct: 327  DGQLCVICLMRRRRSVFIPCGHLVCCQGCAISVEREVAPKCPVCRQEIRDSVRTYES 383


>ref|XP_002274016.1| PREDICTED: E3 ubiquitin-protein ligase SPL2 [Vitis vinifera]
 emb|CBI35868.3| unnamed protein product, partial [Vitis vinifera]
          Length = 391

 Score =  476 bits (1224), Expect = e-161
 Identities = 224/360 (62%), Positives = 289/360 (80%), Gaps = 4/360 (1%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENS--DDGIIVIVRGTVDAK 361
            VRS I+ S+ ++A+R++  +PS  VSDLRSLL+ +  D  + S   DG++V+VRGTV+AK
Sbjct: 33   VRSFIRLSSTSSALRQIDQSPSFRVSDLRSLLSPSSDDESDQSTSSDGLLVVVRGTVEAK 92

Query: 362  SAVEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQE 541
            SA++G+WK+LRP+VLVSHESG+KGVILQRTQTCIYNEW G FGW+SD+R +F R+W+++E
Sbjct: 93   SAIDGNWKSLRPNVLVSHESGEKGVILQRTQTCIYNEWRGFFGWTSDIRTLFARSWKEKE 152

Query: 542  STSLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQAFFWR 718
            STSLR VPF+L + GR   ++YV+VN+DGSRHPLPLTTVYH+L+P+  +PYTFLQA F  
Sbjct: 153  STSLRTVPFILVEGGRWPQSDYVIVNMDGSRHPLPLTTVYHQLQPVNASPYTFLQALFGH 212

Query: 719  QYPIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKT 898
             YP+GLLDEEK+LPLGK+I+AVG+C L++GI EIKSCKDLPYFLSE+SKDQM+VDL FK+
Sbjct: 213  DYPVGLLDEEKLLPLGKEITAVGICSLKNGIPEIKSCKDLPYFLSEMSKDQMVVDLVFKS 272

Query: 899  KTLFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQ-PDDEIE 1075
            K LFWSG++LGSVS+G+LGYA+V                      ++ D D Q  +D+  
Sbjct: 273  KVLFWSGVILGSVSIGVLGYAVV-RNWNRWKEWRQQRGVRPPSQAVTDDTDTQIAEDDAG 331

Query: 1076 DVPDGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DVPDG+LCVICLMRR+RS F+PCGHLVCC  CA+SVE E++PKCPVCRQ IR+SVRI+ S
Sbjct: 332  DVPDGELCVICLMRRKRSAFVPCGHLVCCQRCALSVERELSPKCPVCRQIIRSSVRIYGS 391


>gb|KHN39625.1| Mitochondrial ubiquitin ligase activator of nfkb 1, partial [Glycine
            soja]
          Length = 320

 Score =  469 bits (1208), Expect = e-160
 Identities = 236/326 (72%), Positives = 262/326 (80%), Gaps = 1/326 (0%)
 Frame = +2

Query: 281  LADTVSDADENSDDGIIVIVRGTVDAKSAVEGSWKTLRPSVLVSHESGDKGVILQRTQTC 460
            LA+  SDAD     G IVI+RGTVDAKSAV+G+WKTL+P VLVS ESGDKGVILQRTQTC
Sbjct: 1    LAEPPSDADA----GEIVIIRGTVDAKSAVDGTWKTLKPGVLVSRESGDKGVILQRTQTC 56

Query: 461  IYNEWNGLFGWSSDLRGIFGRTWRQQESTSLRKVPFVLTDDGRRSNTEYVVVNLDGSRHP 640
            IYN+W GL GW+SDLR I  R+ RQQ+STS RKVPFVL D GRR N EYVVVN+DGSRH 
Sbjct: 57   IYNDWKGLLGWTSDLRAICARSLRQQDSTSFRKVPFVLIDVGRRPNAEYVVVNMDGSRHS 116

Query: 641  LPLTTVYHKLEPI-TTPYTFLQAFFWRQYPIGLLDEEKILPLGKDISAVGLCRLRDGIAE 817
            LPLTTVYHKL+PI  +PYTFLQA F  +YP+GLLDEEKILPLGKDI+AVG C L++GIAE
Sbjct: 117  LPLTTVYHKLQPINASPYTFLQALFGHEYPVGLLDEEKILPLGKDITAVGHCSLKNGIAE 176

Query: 818  IKSCKDLPYFLSELSKDQMIVDLSFKTKTLFWSGILLGSVSVGILGYAIVXXXXXXXXXX 997
            IKSCKD+PYFLS+LSKDQMIVDLS K K LFW GI LGS+SVG+LGYA++          
Sbjct: 177  IKSCKDIPYFLSDLSKDQMIVDLSIKAKILFWGGISLGSMSVGVLGYAVLRNWIKWKRWK 236

Query: 998  XXXXXXXXXXXTISGDVDPQPDDEIEDVPDGQLCVICLMRRRRSVFIPCGHLVCCHGCAI 1177
                           D +PQ DDEIEDVPDGQLCVICLMRRRRSVFIPCGHLVCC GCAI
Sbjct: 237  VQRQLQQQRQAV--SDAEPQVDDEIEDVPDGQLCVICLMRRRRSVFIPCGHLVCCQGCAI 294

Query: 1178 SVENEVAPKCPVCRQEIRNSVRIFES 1255
            SVE EVAPKCPVCRQEIR+SVR +ES
Sbjct: 295  SVEREVAPKCPVCRQEIRDSVRTYES 320


>ref|XP_021628506.1| E3 ubiquitin-protein ligase SPL2 isoform X2 [Manihot esculenta]
          Length = 392

 Score =  470 bits (1209), Expect = e-159
 Identities = 232/365 (63%), Positives = 283/365 (77%), Gaps = 10/365 (2%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGI-------IVIVRG 346
            VRS +KF++N+ A+RK+  AP+L VSDLRS+L D    +DEN +  +       +VIVRG
Sbjct: 33   VRSFLKFTSNSKALRKISKAPTLRVSDLRSVL-DLPQPSDENQNQNVDQGQTQKLVIVRG 91

Query: 347  TVDAKSAVEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRT 526
             V+ KSAV+G+WK+LR + LVSHE GDK VI+QRTQTCIYNEW G FGW+SD+R IFGR+
Sbjct: 92   QVEPKSAVDGNWKSLRSNALVSHECGDKAVIIQRTQTCIYNEWKGFFGWTSDIRAIFGRS 151

Query: 527  WRQQESTSLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQ 703
            WR+QESTSLR VPFVL + GR   ++YV+VN+DGSRH LPLTTVYH+L+PI  +PYTFLQ
Sbjct: 152  WREQESTSLRMVPFVLVEGGRWPQSDYVIVNIDGSRHALPLTTVYHQLQPIDASPYTFLQ 211

Query: 704  AFFWRQYPIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVD 883
            A F  +YP+GLLDEEKILPLGK+I+AVG+C  R+G+ EIKSCKDLPYFLS+++KDQM+VD
Sbjct: 212  ALFGHEYPVGLLDEEKILPLGKEINAVGICSSRNGVLEIKSCKDLPYFLSDMTKDQMVVD 271

Query: 884  LSFKTKTLFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQ-- 1057
            L+FKTK L WSG++LGS+S+GILGYA V                       S D DPQ  
Sbjct: 272  LAFKTKVLLWSGVVLGSLSIGILGYAAV-----RWKEWRQHRQSQQQNHAASTDDDPQID 326

Query: 1058 PDDEIEDVPDGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNS 1237
             D+E  DVPDGQLCVICLMRRRRS FIPCGHLVCC  CAISVE EV+PKCP+CRQ +RNS
Sbjct: 327  ADEEAGDVPDGQLCVICLMRRRRSAFIPCGHLVCCQLCAISVEREVSPKCPLCRQAVRNS 386

Query: 1238 VRIFE 1252
            +RIFE
Sbjct: 387  MRIFE 391


>ref|XP_021628505.1| E3 ubiquitin-protein ligase SPL2 isoform X1 [Manihot esculenta]
 gb|OAY36412.1| hypothetical protein MANES_11G019800 [Manihot esculenta]
          Length = 396

 Score =  470 bits (1209), Expect = e-159
 Identities = 232/365 (63%), Positives = 283/365 (77%), Gaps = 10/365 (2%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGI-------IVIVRG 346
            VRS +KF++N+ A+RK+  AP+L VSDLRS+L D    +DEN +  +       +VIVRG
Sbjct: 33   VRSFLKFTSNSKALRKISKAPTLRVSDLRSVL-DLPQPSDENQNQNVDQGQTQKLVIVRG 91

Query: 347  TVDAKSAVEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRT 526
             V+ KSAV+G+WK+LR + LVSHE GDK VI+QRTQTCIYNEW G FGW+SD+R IFGR+
Sbjct: 92   QVEPKSAVDGNWKSLRSNALVSHECGDKAVIIQRTQTCIYNEWKGFFGWTSDIRAIFGRS 151

Query: 527  WRQQESTSLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQ 703
            WR+QESTSLR VPFVL + GR   ++YV+VN+DGSRH LPLTTVYH+L+PI  +PYTFLQ
Sbjct: 152  WREQESTSLRMVPFVLVEGGRWPQSDYVIVNIDGSRHALPLTTVYHQLQPIDASPYTFLQ 211

Query: 704  AFFWRQYPIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVD 883
            A F  +YP+GLLDEEKILPLGK+I+AVG+C  R+G+ EIKSCKDLPYFLS+++KDQM+VD
Sbjct: 212  ALFGHEYPVGLLDEEKILPLGKEINAVGICSSRNGVLEIKSCKDLPYFLSDMTKDQMVVD 271

Query: 884  LSFKTKTLFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQ-- 1057
            L+FKTK L WSG++LGS+S+GILGYA V                       S D DPQ  
Sbjct: 272  LAFKTKVLLWSGVVLGSLSIGILGYAAV-RNWNRWKEWRQHRQSQQQNHAASTDDDPQID 330

Query: 1058 PDDEIEDVPDGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNS 1237
             D+E  DVPDGQLCVICLMRRRRS FIPCGHLVCC  CAISVE EV+PKCP+CRQ +RNS
Sbjct: 331  ADEEAGDVPDGQLCVICLMRRRRSAFIPCGHLVCCQLCAISVEREVSPKCPLCRQAVRNS 390

Query: 1238 VRIFE 1252
            +RIFE
Sbjct: 391  MRIFE 395


>ref|XP_012068880.1| E3 ubiquitin-protein ligase SPL2 [Jatropha curcas]
 gb|KDP40699.1| hypothetical protein JCGZ_24698 [Jatropha curcas]
          Length = 391

 Score =  466 bits (1199), Expect = e-157
 Identities = 226/360 (62%), Positives = 284/360 (78%), Gaps = 5/360 (1%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSDDGI--IVIVRGTVDAK 361
            VRS +KF++N+ A+RK+  AP++ VSDLRS+L D    ADEN +     +VIV+G V+AK
Sbjct: 33   VRSFLKFTSNSKALRKIRSAPTVKVSDLRSIL-DLSQRADENQNQNNQKLVIVQGQVEAK 91

Query: 362  SAVEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQE 541
            S V+ +WK+ RP+VL++HESGDK VI+QRTQTC+YNEW G FGW+SDLR IFGR+WR+QE
Sbjct: 92   SNVDSNWKSYRPNVLIAHESGDKAVIVQRTQTCLYNEWKGFFGWTSDLRAIFGRSWREQE 151

Query: 542  STSLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPI-TTPYTFLQAFFWR 718
            STSLR VPFVL + GR  ++ YV+VN+DGSRHPLPLTTVYH+L+PI  +PYTFLQA F  
Sbjct: 152  STSLRTVPFVLVESGRWQHSNYVIVNMDGSRHPLPLTTVYHQLQPIDASPYTFLQALFGH 211

Query: 719  QYPIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFKT 898
            +YP+GLLDEEKILPLGK+I+ VG+   ++GI+EIKSCKDLPYFLS+++KDQM+VDL+FKT
Sbjct: 212  EYPVGLLDEEKILPLGKEINIVGIVSFKNGISEIKSCKDLPYFLSDMTKDQMVVDLAFKT 271

Query: 899  KTLFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIE- 1075
            K L WSGI+LGS+S+GILGYA+V                        GD  PQ D ++E 
Sbjct: 272  KVLLWSGIVLGSLSIGILGYAVVRNWNKWKEWRQQRQSQQQSHALSDGDA-PQIDADVET 330

Query: 1076 -DVPDGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFE 1252
             DVPDGQLCVICLMRRRRS F+PCGHLVCC  CAISVE E++PKCP+CRQ IR+S+RIF+
Sbjct: 331  GDVPDGQLCVICLMRRRRSAFVPCGHLVCCQLCAISVEREISPKCPLCRQAIRSSMRIFD 390


>ref|XP_010086805.1| E3 ubiquitin-protein ligase SPL2 [Morus notabilis]
 gb|EXB23815.1| Mitochondrial ubiquitin ligase activator of nfkb 1 [Morus notabilis]
          Length = 393

 Score =  466 bits (1199), Expect = e-157
 Identities = 229/362 (63%), Positives = 282/362 (77%), Gaps = 6/362 (1%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLADTVSDADENSD---DGIIVIVRGTVDA 358
            VRS++K+++ ++A+ KL  APS+ VSDLRS+LA   S    N     DG +VIVRGTV+A
Sbjct: 33   VRSVLKYTSTSSALCKLRKAPSVQVSDLRSILAADASSEGSNESRPSDGKLVIVRGTVEA 92

Query: 359  KSAVEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQ 538
            KSA++GSWK+LRP+VLVS ESGDK V++QRTQTCIYNEW G FGW+SDLR IF R+WR+Q
Sbjct: 93   KSAIDGSWKSLRPNVLVSQESGDKAVVIQRTQTCIYNEWKGFFGWTSDLRTIFARSWREQ 152

Query: 539  ESTSLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPIT-TPYTFLQAFFW 715
            ESTSLR V F+L + G+    ++VVVN+DGSRHPLPLTTVYH+L+P+  +PYTFLQA F 
Sbjct: 153  ESTSLRTVSFILVEAGKWPQMDFVVVNMDGSRHPLPLTTVYHQLQPVNPSPYTFLQALFG 212

Query: 716  RQYPIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFK 895
             +YP+GLLDEEKILPLGKDI+AVG+C  ++GIAEIKSCKD+PYFL+E++KDQM+VDL+F 
Sbjct: 213  HEYPVGLLDEEKILPLGKDITAVGICSFKNGIAEIKSCKDIPYFLTEMTKDQMVVDLAFS 272

Query: 896  TKTLFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQ--PDDE 1069
            TK L  SGI+LGS+S+GILGYA+V                      I  D + Q   +++
Sbjct: 273  TKVLLVSGIVLGSLSIGILGYALVRNWNRWKEWRHNRQQQRANPPAID-DSESQIVSEED 331

Query: 1070 IEDVPDGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIF 1249
              DVPDGQLCVICL RRRRS FIPCGHLVCCH CAISVE EV PKCPVCRQEIR SVRI+
Sbjct: 332  TTDVPDGQLCVICLTRRRRSAFIPCGHLVCCHICAISVEREVTPKCPVCRQEIRTSVRIY 391

Query: 1250 ES 1255
            +S
Sbjct: 392  DS 393


>gb|PON88937.1| Mitochondrial ubiquitin ligase activator of NFKB [Trema orientalis]
          Length = 393

 Score =  462 bits (1188), Expect = e-156
 Identities = 226/360 (62%), Positives = 281/360 (78%), Gaps = 4/360 (1%)
 Frame = +2

Query: 188  VRSIIKFSANNTAIRKLHDAPSLSVSDLRSLLA---DTVSDADENSDDGIIVIVRGTVDA 358
            +RSIIKF+++++A+ KL  APS+ VSDLRS+LA      SD  +   DG I+IVRGTV+A
Sbjct: 34   IRSIIKFTSSSSALHKLRRAPSVKVSDLRSILAVDDPQSSDESQPQPDGKILIVRGTVEA 93

Query: 359  KSAVEGSWKTLRPSVLVSHESGDKGVILQRTQTCIYNEWNGLFGWSSDLRGIFGRTWRQQ 538
            KSA++G+WK+LRP+VLVS ESGDK V+LQRTQTCIYNEW G  GW+SDLR IF R+WR+Q
Sbjct: 94   KSAIDGNWKSLRPNVLVSKESGDKAVVLQRTQTCIYNEWKGFLGWTSDLRSIFARSWREQ 153

Query: 539  ESTSLRKVPFVLTDDGRRSNTEYVVVNLDGSRHPLPLTTVYHKLEPIT-TPYTFLQAFFW 715
            ESTSLR VPFVL + G+  ++++VVVNLDGSRHPLPLTTVYH+L+P++ +PYTFLQA F 
Sbjct: 154  ESTSLRTVPFVLVEGGKWPHSDFVVVNLDGSRHPLPLTTVYHQLQPVSASPYTFLQALFG 213

Query: 716  RQYPIGLLDEEKILPLGKDISAVGLCRLRDGIAEIKSCKDLPYFLSELSKDQMIVDLSFK 895
             ++P+GLLDEEKILPLGKDI+AVG+C L+DGI EIKSCKDLPYFL+E++KDQM+V+L+F 
Sbjct: 214  HEFPVGLLDEEKILPLGKDITAVGVCSLKDGIPEIKSCKDLPYFLTEMTKDQMVVELAFG 273

Query: 896  TKTLFWSGILLGSVSVGILGYAIVXXXXXXXXXXXXXXXXXXXXXTISGDVDPQPDDEIE 1075
            TK L  SGI+LGS+SVGILGYA+V                               +++  
Sbjct: 274  TKVLLLSGIVLGSLSVGILGYAVVRNWNKLKEWRQRRRFQQPNPPANDAYEFQIVEEDAT 333

Query: 1076 DVPDGQLCVICLMRRRRSVFIPCGHLVCCHGCAISVENEVAPKCPVCRQEIRNSVRIFES 1255
            DVPDG LCVICL RRRRS F+PCGHLVCC  CAI+VE+  APKCPVCRQEIR SVRI++S
Sbjct: 334  DVPDGMLCVICLTRRRRSAFVPCGHLVCCQSCAITVEHAAAPKCPVCRQEIRASVRIYDS 393