BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00010987
         (673 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arieti...   288   2e-86
dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subt...   287   4e-86
gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium prate...   287   6e-86
dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subt...   286   8e-86
sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName:...   285   3e-85
ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago tru...   284   8e-85
ref|XP_023521325.1| DNA topoisomerase 2-like [Cucurbita pepo sub...   259   1e-79
ref|XP_017696425.1| PREDICTED: DNA topoisomerase 2-like [Phoenix...   242   3e-77
ref|XP_022982563.1| DNA topoisomerase 2 isoform X2 [Cucurbita ma...   261   1e-76
ref|XP_022982562.1| DNA topoisomerase 2 isoform X1 [Cucurbita ma...   261   1e-76
dbj|GAU30192.1| hypothetical protein TSUD_311430 [Trifolium subt...   260   2e-76
gb|PON74229.1| DNA topoisomerase II-type [Parasponia andersonii]      259   4e-76
ref|XP_023527826.1| DNA topoisomerase 2 isoform X6 [Cucurbita pe...   259   4e-76
ref|XP_023527825.1| DNA topoisomerase 2 isoform X5 [Cucurbita pe...   259   5e-76
ref|XP_007148908.1| hypothetical protein PHAVU_005G024200g [Phas...   259   5e-76
ref|XP_022935016.1| DNA topoisomerase 2 isoform X2 [Cucurbita mo...   259   5e-76
ref|XP_022935015.1| DNA topoisomerase 2 isoform X1 [Cucurbita mo...   259   5e-76
gb|PON46642.1| DNA topoisomerase II-type [Trema orientalis]           259   6e-76
ref|XP_023527823.1| DNA topoisomerase 2 isoform X3 [Cucurbita pe...   259   6e-76
ref|XP_023527820.1| DNA topoisomerase 2 isoform X1 [Cucurbita pe...   259   6e-76

>ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
 ref|XP_004517303.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
          Length = 1472

 Score =  288 bits (738), Expect = 2e-86
 Identities = 149/224 (66%), Positives = 175/224 (78%), Gaps = 19/224 (8%)
 Frame = -2

Query: 615 MEKPPLRTSNAANIPSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKKDEIVY 442
           MEK PL+TSNA NIPS+T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LW+++ DE+V+
Sbjct: 1   MEKRPLQTSNATNIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYENDEMVH 60

Query: 441 RSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPEEVDQE 262
           RSISYVPGLYKIFD ILV+A DNK RDPSMDSLKVTIDP ANTVS+Y+N +GVP E+ QE
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQE 120

Query: 261 EKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCHLKKYK 133
           EK YVPELI                   GRNGY GAKLT IFSTE +IETADG  LKKYK
Sbjct: 121 EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGY-GAKLTNIFSTEFVIETADGRRLKKYK 179

Query: 132 QAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           Q F+NNM  +S+P IT CK +ENWTKVTFKPDLEKF+MT+LEED
Sbjct: 180 QVFSNNMGNKSEPVITKCKSSENWTKVTFKPDLEKFKMTYLEED 223


>dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subterraneum]
          Length = 1336

 Score =  287 bits (735), Expect = 4e-86
 Identities = 149/224 (66%), Positives = 173/224 (77%), Gaps = 19/224 (8%)
 Frame = -2

Query: 615 MEKPPLRTSNAANIPSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKKDEIVY 442
           MEK PL+TSNA NIPS+T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LWV++ DE+V 
Sbjct: 1   MEKRPLQTSNATNIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVQ 60

Query: 441 RSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPEEVDQE 262
           RSISYVPGLYKIFD ILV+A DNK RDPSMDSLKVTIDP  NT+S+Y+N +GVP E+ QE
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPERNTISVYNNGDGVPVEIHQE 120

Query: 261 EKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCHLKKYK 133
           EK YVPELI                   GRNGY GAKLT IFSTE +IETADG  LKKYK
Sbjct: 121 EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGY-GAKLTNIFSTEFVIETADGRRLKKYK 179

Query: 132 QAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           Q F+NNM  +S+P IT CK NENWTKVTFKPDLEKF+MT+LEED
Sbjct: 180 QVFSNNMGTKSEPVITKCKANENWTKVTFKPDLEKFKMTYLEED 223


>gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium pratense]
          Length = 1492

 Score =  287 bits (735), Expect = 6e-86
 Identities = 151/224 (67%), Positives = 175/224 (78%), Gaps = 19/224 (8%)
 Frame = -2

Query: 615 MEKPPLRTSNAANIPSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKKDEIVY 442
           MEK PL+TSNAANIPS+T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LWV++ DE+V 
Sbjct: 1   MEKRPLQTSNAANIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVN 60

Query: 441 RSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPEEVDQE 262
           RSISYVPGLYKIFD ILV+A DNK RDPSMDSLKVTIDP ANTVS+Y+N +GVP E+ QE
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQE 120

Query: 261 EKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCHLKKYK 133
           EK YVPELI                   GRNGY GAKLT IFSTE IIETADG  LKKYK
Sbjct: 121 EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGY-GAKLTNIFSTEFIIETADGRRLKKYK 179

Query: 132 QAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           Q F++NM  +S+P IT CK +ENWTKVTFKPDLEKF+MT+LEED
Sbjct: 180 QVFSDNMGKKSEPVITKCKASENWTKVTFKPDLEKFKMTYLEED 223


>dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subterraneum]
          Length = 1359

 Score =  286 bits (733), Expect = 8e-86
 Identities = 150/224 (66%), Positives = 174/224 (77%), Gaps = 19/224 (8%)
 Frame = -2

Query: 615 MEKPPLRTSNAANIPSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKKDEIVY 442
           MEK PL+TSNA NIPS+T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LWV++ DE+V 
Sbjct: 1   MEKRPLQTSNATNIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVN 60

Query: 441 RSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPEEVDQE 262
           RSISYVPGLYKIFD ILV+A DNK RDPSMDSLKVTIDP ANTVS+Y+N +GVP E+ QE
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQE 120

Query: 261 EKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCHLKKYK 133
           EK YVPELI                   GRNGY GAKLT IFSTE IIETADG  LKKYK
Sbjct: 121 EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGY-GAKLTNIFSTEFIIETADGRRLKKYK 179

Query: 132 QAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           Q F+NNM  +++P IT CK +ENWTKVTFKPDLEKF+MT+LEED
Sbjct: 180 QVFSNNMGKKAEPVITKCKASENWTKVTFKPDLEKFKMTYLEED 223


>sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase
           II; AltName: Full=PsTopII
 emb|CAA74891.1| topoisomerase II [Pisum sativum]
          Length = 1462

 Score =  285 bits (730), Expect = 3e-85
 Identities = 148/224 (66%), Positives = 174/224 (77%), Gaps = 19/224 (8%)
 Frame = -2

Query: 615 MEKPPLRTSNAANIPSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKKDEIVY 442
           MEK PL+TSNAANIPS+T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LWV++ DE+V+
Sbjct: 1   MEKRPLQTSNAANIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVH 60

Query: 441 RSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPEEVDQE 262
           R++SYVPGLYKIFD ILV+A DNK RDPSMDSLKVTIDP ANTVS+Y+N +GVP E+ QE
Sbjct: 61  RAVSYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQE 120

Query: 261 EKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCHLKKYK 133
           EK YVPELI                   GRNGY GAKLT IFSTE IIETADG  LKKYK
Sbjct: 121 EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGY-GAKLTNIFSTEFIIETADGRRLKKYK 179

Query: 132 QAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           Q F+NNM  + +P IT CK +ENWTKVTFKPDLEKF+M +LEED
Sbjct: 180 QVFSNNMGTKCEPVITKCKASENWTKVTFKPDLEKFKMAYLEED 223


>ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago truncatula]
 gb|AES69522.2| DNA topoisomerase IV subunit A [Medicago truncatula]
          Length = 1504

 Score =  284 bits (727), Expect = 8e-85
 Identities = 149/224 (66%), Positives = 172/224 (76%), Gaps = 19/224 (8%)
 Frame = -2

Query: 615 MEKPPLRTSNAANIPSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKKDEIVY 442
           MEK PL+ S  ANIPS+T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LWV++ +E+V 
Sbjct: 1   MEKRPLQPSTTANIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENEEMVN 60

Query: 441 RSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPEEVDQE 262
           RSISYVPGLYKIFD ILV+A DNK RDPSMDSLKVTIDP ANTVS+Y+N +GVP E+ QE
Sbjct: 61  RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQE 120

Query: 261 EKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCHLKKYK 133
           EK YVPELI                   GRNGY GAKLT IFSTE +IETADG  LKKYK
Sbjct: 121 EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGY-GAKLTNIFSTEFVIETADGRRLKKYK 179

Query: 132 QAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           Q FT+NM  +SDP IT CK NENWTKVTFKPDLEKF+MT+LEED
Sbjct: 180 QVFTDNMGKKSDPVITKCKANENWTKVTFKPDLEKFKMTYLEED 223


>ref|XP_023521325.1| DNA topoisomerase 2-like [Cucurbita pepo subsp. pepo]
          Length = 615

 Score =  259 bits (661), Expect = 1e-79
 Identities = 135/229 (58%), Positives = 167/229 (72%), Gaps = 25/229 (10%)
 Frame = -2

Query: 612 EKPPLRTSNAANI------PSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKK 457
           +K PL+ SNAAN+      P +T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LW+++ 
Sbjct: 5   KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 456 DEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPE 277
           D++V+RS++YVPGLYKIFD ILV+A DNK RDPSMDSLKV ID   N +S+++N +GVP 
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGDGVPV 124

Query: 276 EVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCH 148
           EV QEEK YVPELI                   GRNGY GAKLT IFSTE +IETADG  
Sbjct: 125 EVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGY-GAKLTNIFSTEFVIETADGRR 183

Query: 147 LKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           LKKYKQ F++NM  +S+P IT CKE +NWTKVTFKPDL KF MTHLE+D
Sbjct: 184 LKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDD 232


>ref|XP_017696425.1| PREDICTED: DNA topoisomerase 2-like [Phoenix dactylifera]
          Length = 263

 Score =  242 bits (618), Expect = 3e-77
 Identities = 125/233 (53%), Positives = 159/233 (68%), Gaps = 29/233 (12%)
 Frame = -2

Query: 612 EKPPLRTSNAAN----------IPSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLW 469
           +K PL++S A N          +P +T   ++++ TQ EHIL  P+ Y+GS+EKHTQ LW
Sbjct: 5   KKQPLQSSTAYNSTPGGGGAAPVPKKTIEEVYQKKTQLEHILLRPDTYIGSVEKHTQTLW 64

Query: 468 VFKKDEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRN 289
           V++  E+V+R ++YVPGLYKIFD ILV+A DNK RDPSMDS+KV ID   N +S+Y+N +
Sbjct: 65  VYENGEMVHRPVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVEIDVAGNCISVYNNGD 124

Query: 288 GVPEEVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETA 160
           G+P E+ QEE  YVPE+I                   GRNGY GAKL+ IFSTE +I+TA
Sbjct: 125 GIPVEIHQEEGVYVPEMIFGHLLTSSNYDDNVKKTTGGRNGY-GAKLSNIFSTEFVIQTA 183

Query: 159 DGCHLKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           DG   KKYKQ F+ NM  +S+P I  CKE ENWTKVTFKPDL KF MTHLE+D
Sbjct: 184 DGRRQKKYKQVFSENMGKKSEPSIVKCKEGENWTKVTFKPDLAKFNMTHLEDD 236


>ref|XP_022982563.1| DNA topoisomerase 2 isoform X2 [Cucurbita maxima]
          Length = 1490

 Score =  261 bits (667), Expect = 1e-76
 Identities = 135/230 (58%), Positives = 167/230 (72%), Gaps = 25/230 (10%)
 Frame = -2

Query: 615 MEKPPLRTSNAANIPSETF--------IFREMTQREHILRHPNIYVGSMEKHTQDLWVFK 460
           +EK PL+ SNAAN+ S+          ++++ TQ EHIL  P+ YVGS+EKHTQ+LW+++
Sbjct: 3   VEKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYE 62

Query: 459 KDEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVP 280
            D++V+RS++YVPGLYKIFD ILV+A DNK RDPSMDSLKV ID   N +S+++N +GVP
Sbjct: 63  NDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNCISVFNNGDGVP 122

Query: 279 EEVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGC 151
            EV QEEK YVPELI                   GRNGY GAKLT IFSTE +IETADG 
Sbjct: 123 VEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGY-GAKLTNIFSTEFVIETADGR 181

Query: 150 HLKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
            LKKYKQ F++NM  +S+P IT CKE +NWTKVTFKPDL KF MTHLE+D
Sbjct: 182 RLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDD 231


>ref|XP_022982562.1| DNA topoisomerase 2 isoform X1 [Cucurbita maxima]
          Length = 1491

 Score =  261 bits (667), Expect = 1e-76
 Identities = 135/230 (58%), Positives = 167/230 (72%), Gaps = 25/230 (10%)
 Frame = -2

Query: 615 MEKPPLRTSNAANIPSETF--------IFREMTQREHILRHPNIYVGSMEKHTQDLWVFK 460
           +EK PL+ SNAAN+ S+          ++++ TQ EHIL  P+ YVGS+EKHTQ+LW+++
Sbjct: 3   VEKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYE 62

Query: 459 KDEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVP 280
            D++V+RS++YVPGLYKIFD ILV+A DNK RDPSMDSLKV ID   N +S+++N +GVP
Sbjct: 63  NDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNCISVFNNGDGVP 122

Query: 279 EEVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGC 151
            EV QEEK YVPELI                   GRNGY GAKLT IFSTE +IETADG 
Sbjct: 123 VEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGY-GAKLTNIFSTEFVIETADGR 181

Query: 150 HLKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
            LKKYKQ F++NM  +S+P IT CKE +NWTKVTFKPDL KF MTHLE+D
Sbjct: 182 RLKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDD 231


>dbj|GAU30192.1| hypothetical protein TSUD_311430 [Trifolium subterraneum]
          Length = 1252

 Score =  260 bits (664), Expect = 2e-76
 Identities = 132/203 (65%), Positives = 156/203 (76%), Gaps = 17/203 (8%)
 Frame = -2

Query: 558 IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKKDEIVYRSISYVPGLYKIFDAILVDAV 379
           ++++ TQ EHIL  P+ YVGS+EKHTQ+LWV++ +E+V+RSISYVPGLYKIFD ILV+A 
Sbjct: 8   VYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENEEMVHRSISYVPGLYKIFDEILVNAA 67

Query: 378 DNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPEEVDQEEKAYVPELIL----------- 232
           DNK RD SMDSLKVTIDP  NT+SIY+N +G+P E+ QEEK YVPELI            
Sbjct: 68  DNKQRDSSMDSLKVTIDPELNTISIYNNGDGIPVEIHQEEKVYVPELIFGHLLTSSNYDD 127

Query: 231 ------DGRNGYGGAKLTIIFSTECIIETADGCHLKKYKQAFTNNMEMESDPEITICKEN 70
                  GRNGY GAKLT IFSTE +IETADG  LKKYKQ F+NNM  +S P IT CK N
Sbjct: 128 NVKKTTGGRNGY-GAKLTNIFSTEFVIETADGRRLKKYKQVFSNNMGKKSKPVITKCKAN 186

Query: 69  ENWTKVTFKPDLEKFEMTHLEED 1
           +NWTKVTFKPDLEKF+MT+LEED
Sbjct: 187 QNWTKVTFKPDLEKFKMTYLEED 209


>gb|PON74229.1| DNA topoisomerase II-type [Parasponia andersonii]
          Length = 1397

 Score =  259 bits (662), Expect = 4e-76
 Identities = 137/237 (57%), Positives = 168/237 (70%), Gaps = 33/237 (13%)
 Frame = -2

Query: 612 EKPPLRTSNAANI--------------PSETF--IFREMTQREHILRHPNIYVGSMEKHT 481
           +K PL++SN AN+              P++T   ++++ +Q EHIL  P+ Y+GS+EKHT
Sbjct: 5   KKRPLQSSNNANMAPASTVDAAAAGGGPAKTIEEMYQKKSQLEHILLRPDTYIGSIEKHT 64

Query: 480 QDLWVFKKDEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIY 301
           Q LWV++ DE+V+RS++YVPGLYKIFD ILV+A DNK RDPSMDS+KV ID   N +S+Y
Sbjct: 65  QTLWVYQNDEMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVY 124

Query: 300 HNRNGVPEEVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECI 172
           +N +GVP E+ QEEK YVPELI                   GRNGY GAKLT IFSTE +
Sbjct: 125 NNGDGVPVEIHQEEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGY-GAKLTNIFSTEFV 183

Query: 171 IETADGCHLKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           IETADG  LKKYKQ F+NNM  +SDP IT CKE+ENWTKVTFKPDL KF MTHLEED
Sbjct: 184 IETADGKRLKKYKQVFSNNMGKKSDPVITKCKESENWTKVTFKPDLAKFNMTHLEED 240


>ref|XP_023527826.1| DNA topoisomerase 2 isoform X6 [Cucurbita pepo subsp. pepo]
          Length = 1268

 Score =  259 bits (661), Expect = 4e-76
 Identities = 135/229 (58%), Positives = 167/229 (72%), Gaps = 25/229 (10%)
 Frame = -2

Query: 612 EKPPLRTSNAANI------PSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKK 457
           +K PL+ SNAAN+      P +T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LW+++ 
Sbjct: 5   KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 456 DEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPE 277
           D++V+RS++YVPGLYKIFD ILV+A DNK RDPSMDSLKV ID   N +S+++N +GVP 
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGDGVPV 124

Query: 276 EVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCH 148
           EV QEEK YVPELI                   GRNGY GAKLT IFSTE +IETADG  
Sbjct: 125 EVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGY-GAKLTNIFSTEFVIETADGRR 183

Query: 147 LKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           LKKYKQ F++NM  +S+P IT CKE +NWTKVTFKPDL KF MTHLE+D
Sbjct: 184 LKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDD 232


>ref|XP_023527825.1| DNA topoisomerase 2 isoform X5 [Cucurbita pepo subsp. pepo]
          Length = 1269

 Score =  259 bits (661), Expect = 5e-76
 Identities = 135/229 (58%), Positives = 167/229 (72%), Gaps = 25/229 (10%)
 Frame = -2

Query: 612 EKPPLRTSNAANI------PSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKK 457
           +K PL+ SNAAN+      P +T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LW+++ 
Sbjct: 5   KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 456 DEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPE 277
           D++V+RS++YVPGLYKIFD ILV+A DNK RDPSMDSLKV ID   N +S+++N +GVP 
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGDGVPV 124

Query: 276 EVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCH 148
           EV QEEK YVPELI                   GRNGY GAKLT IFSTE +IETADG  
Sbjct: 125 EVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGY-GAKLTNIFSTEFVIETADGRR 183

Query: 147 LKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           LKKYKQ F++NM  +S+P IT CKE +NWTKVTFKPDL KF MTHLE+D
Sbjct: 184 LKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDD 232


>ref|XP_007148908.1| hypothetical protein PHAVU_005G024200g [Phaseolus vulgaris]
 gb|ESW20902.1| hypothetical protein PHAVU_005G024200g [Phaseolus vulgaris]
          Length = 1474

 Score =  259 bits (662), Expect = 5e-76
 Identities = 137/225 (60%), Positives = 162/225 (72%), Gaps = 22/225 (9%)
 Frame = -2

Query: 609 KPPLRTSNAANIPSETF-----IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKKDEIV 445
           K PL++S+AAN+ +        ++++ TQ EHIL  P+ YVGS+EKHTQ LWV++ DE+V
Sbjct: 6   KRPLQSSSAANVAAAAGKTIEEVYQKKTQLEHILLRPDTYVGSIEKHTQTLWVYENDEMV 65

Query: 444 YRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPEEVDQ 265
            R ISYVPGLYKIFD ILV+A DNK RDPSMD+LKVTID   NTVS+++N +GVP E+ Q
Sbjct: 66  QRPISYVPGLYKIFDEILVNAADNKQRDPSMDALKVTIDAEQNTVSVFNNGDGVPIEIHQ 125

Query: 264 EEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCHLKKY 136
           EEK YVPELI                   GRNGY GAKLT IFSTE +IETADG  LKKY
Sbjct: 126 EEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGY-GAKLTNIFSTEFVIETADGKRLKKY 184

Query: 135 KQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           KQ F+NNM  +S+P IT CK  ENWTKVTFKPDL KF M HLEED
Sbjct: 185 KQVFSNNMGKKSEPVITKCKAGENWTKVTFKPDLAKFRMDHLEED 229


>ref|XP_022935016.1| DNA topoisomerase 2 isoform X2 [Cucurbita moschata]
          Length = 1491

 Score =  259 bits (662), Expect = 5e-76
 Identities = 135/229 (58%), Positives = 166/229 (72%), Gaps = 25/229 (10%)
 Frame = -2

Query: 612 EKPPLRTSNAANIPSETF--------IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKK 457
           +K PL+ SNAAN+ S+          ++++ TQ EHIL  P+ YVGS+EKHTQ+LWV++ 
Sbjct: 5   KKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN 64

Query: 456 DEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPE 277
           D++V+RS++YVPGLYKIFD ILV+A DNK RDPSMDSLKV ID   N +S+++N +GVP 
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGDGVPV 124

Query: 276 EVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCH 148
           EV QEEK YVPELI                   GRNGY GAKLT IFSTE +IETADG  
Sbjct: 125 EVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGY-GAKLTNIFSTEFVIETADGRR 183

Query: 147 LKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           LKKYKQ F++NM  +S+P IT CKE +NWTKVTFKPDL KF MTHLE+D
Sbjct: 184 LKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDD 232


>ref|XP_022935015.1| DNA topoisomerase 2 isoform X1 [Cucurbita moschata]
          Length = 1492

 Score =  259 bits (662), Expect = 5e-76
 Identities = 135/229 (58%), Positives = 166/229 (72%), Gaps = 25/229 (10%)
 Frame = -2

Query: 612 EKPPLRTSNAANIPSETF--------IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKK 457
           +K PL+ SNAAN+ S+          ++++ TQ EHIL  P+ YVGS+EKHTQ+LWV++ 
Sbjct: 5   KKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYEN 64

Query: 456 DEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPE 277
           D++V+RS++YVPGLYKIFD ILV+A DNK RDPSMDSLKV ID   N +S+++N +GVP 
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGDGVPV 124

Query: 276 EVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCH 148
           EV QEEK YVPELI                   GRNGY GAKLT IFSTE +IETADG  
Sbjct: 125 EVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGY-GAKLTNIFSTEFVIETADGRR 183

Query: 147 LKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           LKKYKQ F++NM  +S+P IT CKE +NWTKVTFKPDL KF MTHLE+D
Sbjct: 184 LKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDD 232


>gb|PON46642.1| DNA topoisomerase II-type [Trema orientalis]
          Length = 1397

 Score =  259 bits (661), Expect = 6e-76
 Identities = 137/237 (57%), Positives = 167/237 (70%), Gaps = 33/237 (13%)
 Frame = -2

Query: 612 EKPPLRTSNAANI--------------PSETF--IFREMTQREHILRHPNIYVGSMEKHT 481
           +K PL++SN AN+              P +T   ++++ +Q EHIL  P+ Y+GS+EKHT
Sbjct: 5   KKRPLQSSNNANMAPASTVDAAAAGGGPGKTIEEMYQKKSQLEHILLRPDTYIGSIEKHT 64

Query: 480 QDLWVFKKDEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIY 301
           Q LWV++ DE+V+RS++YVPGLYKIFD ILV+A DNK RDPSMDS+KV ID   N +S+Y
Sbjct: 65  QTLWVYQNDEMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSVKVVIDVEQNCISVY 124

Query: 300 HNRNGVPEEVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECI 172
           +N +GVP E+ QEEK YVPELI                   GRNGY GAKLT IFSTE +
Sbjct: 125 NNGDGVPVEIHQEEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGY-GAKLTNIFSTEFV 183

Query: 171 IETADGCHLKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           IETADG  LKKYKQ F+NNM  +SDP IT CKE+ENWTKVTFKPDL KF MTHLEED
Sbjct: 184 IETADGKRLKKYKQVFSNNMGKKSDPVITKCKESENWTKVTFKPDLAKFNMTHLEED 240


>ref|XP_023527823.1| DNA topoisomerase 2 isoform X3 [Cucurbita pepo subsp. pepo]
 ref|XP_023527824.1| DNA topoisomerase 2 isoform X4 [Cucurbita pepo subsp. pepo]
          Length = 1490

 Score =  259 bits (661), Expect = 6e-76
 Identities = 135/229 (58%), Positives = 167/229 (72%), Gaps = 25/229 (10%)
 Frame = -2

Query: 612 EKPPLRTSNAANI------PSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKK 457
           +K PL+ SNAAN+      P +T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LW+++ 
Sbjct: 5   KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 456 DEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPE 277
           D++V+RS++YVPGLYKIFD ILV+A DNK RDPSMDSLKV ID   N +S+++N +GVP 
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGDGVPV 124

Query: 276 EVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCH 148
           EV QEEK YVPELI                   GRNGY GAKLT IFSTE +IETADG  
Sbjct: 125 EVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGY-GAKLTNIFSTEFVIETADGRR 183

Query: 147 LKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           LKKYKQ F++NM  +S+P IT CKE +NWTKVTFKPDL KF MTHLE+D
Sbjct: 184 LKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDD 232


>ref|XP_023527820.1| DNA topoisomerase 2 isoform X1 [Cucurbita pepo subsp. pepo]
 ref|XP_023527822.1| DNA topoisomerase 2 isoform X2 [Cucurbita pepo subsp. pepo]
          Length = 1491

 Score =  259 bits (661), Expect = 6e-76
 Identities = 135/229 (58%), Positives = 167/229 (72%), Gaps = 25/229 (10%)
 Frame = -2

Query: 612 EKPPLRTSNAANI------PSETF--IFREMTQREHILRHPNIYVGSMEKHTQDLWVFKK 457
           +K PL+ SNAAN+      P +T   ++++ TQ EHIL  P+ YVGS+EKHTQ+LW+++ 
Sbjct: 5   KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 456 DEIVYRSISYVPGLYKIFDAILVDAVDNKLRDPSMDSLKVTIDPGANTVSIYHNRNGVPE 277
           D++V+RS++YVPGLYKIFD ILV+A DNK RDPSMDSLKV ID   N +S+++N +GVP 
Sbjct: 65  DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGDGVPV 124

Query: 276 EVDQEEKAYVPELIL-----------------DGRNGYGGAKLTIIFSTECIIETADGCH 148
           EV QEEK YVPELI                   GRNGY GAKLT IFSTE +IETADG  
Sbjct: 125 EVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGY-GAKLTNIFSTEFVIETADGRR 183

Query: 147 LKKYKQAFTNNMEMESDPEITICKENENWTKVTFKPDLEKFEMTHLEED 1
           LKKYKQ F++NM  +S+P IT CKE +NWTKVTFKPDL KF MTHLE+D
Sbjct: 184 LKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHLEDD 232