BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00007018
         (1022 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AHG97656.1| isoflavone-2'-hydroxylase [Astragalus mongholicus]     683   0.0  
ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phas...   579   0.0  
ref|XP_014506446.2| isoflavone 2'-hydroxylase [Vigna radiata var...   579   0.0  
ref|XP_017405614.1| PREDICTED: isoflavone 2'-hydroxylase-like [V...   578   0.0  
gb|KOM25514.1| hypothetical protein LR48_Vigan107s003300 [Vigna ...   578   0.0  
ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   573   0.0  
dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]                553   0.0  
dbj|BAT21113.1| cytochrome P450 [synthetic construct]                 551   0.0  
sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase;...   551   0.0  
ref|NP_001296643.1| isoflavone 2'-hydroxylase-like [Cicer arieti...   550   0.0  
ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago ...   548   0.0  
ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum] ...   545   0.0  
ref|XP_004504637.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   542   0.0  
ref|XP_004504638.1| PREDICTED: isoflavone 2'-hydroxylase-like [C...   540   0.0  
dbj|BAT21114.1| cytochrome P450 [synthetic construct]                 535   0.0  
dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]                     535   0.0  
ref|XP_016191270.1| isoflavone 2'-hydroxylase [Arachis ipaensis]      528   0.0  
ref|XP_015957959.1| isoflavone 2'-hydroxylase [Arachis duranensis]    528   0.0  
gb|OIW14266.1| hypothetical protein TanjilG_21406 [Lupinus angus...   481   e-166
ref|XP_019439320.1| PREDICTED: isoflavone 2'-hydroxylase-like [L...   481   e-165

>gb|AHG97656.1| isoflavone-2'-hydroxylase [Astragalus mongholicus]
          Length = 498

 Score =  683 bits (1763), Expect = 0.0
 Identities = 339/340 (99%), Positives = 339/340 (99%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ
Sbjct: 98   NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA DSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL
Sbjct: 158  KLAEDSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL
Sbjct: 218  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG
Sbjct: 278  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW
Sbjct: 338  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP
Sbjct: 398  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 437


>ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
 gb|ESW30851.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
          Length = 500

 Score =  579 bits (1492), Expect = 0.0
 Identities = 276/340 (81%), Positives = 309/340 (90%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGSS YG HWRNLRRITA ++LST RIN+FA IR DE HRL++
Sbjct: 100  NRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTQRINTFAAIRRDETHRLIR 159

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA  +S DF EVELTSKFYDMTFNNIMRMISGKRYYGDDCDM DVEEA QFRAMVS+LL
Sbjct: 160  KLAEGASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMADVEEARQFRAMVSELL 219

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            KLSGANNKNDF+P+LRL D+ENLEKRL KI  K D FL GL+EEHRSKK+  NTMVDHLL
Sbjct: 220  KLSGANNKNDFMPLLRLFDFENLEKRLKKISSKTDTFLNGLLEEHRSKKQRANTMVDHLL 279

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            SLQESQPEYYTDQIIKGL  G+L+AGTDSSAVTLEWALSCV+N+PEV+K+ RDEL+TH+G
Sbjct: 280  SLQESQPEYYTDQIIKGLALGMLIAGTDSSAVTLEWALSCVVNHPEVMKRARDELETHVG 339

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            ++RLL+ESDL K+PYLKNI++ETLRLYTPAPL LPHSSSEEC I G+KVPRD+I+L NAW
Sbjct: 340  EERLLDESDLSKLPYLKNIIFETLRLYTPAPLLLPHSSSEECNIGGFKVPRDSIVLINAW 399

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            +IHRDP+VWSEATSFKPERF+ EGELEKLIAFGLGRRACP
Sbjct: 400  SIHRDPEVWSEATSFKPERFENEGELEKLIAFGLGRRACP 439


>ref|XP_014506446.2| isoflavone 2'-hydroxylase [Vigna radiata var. radiata]
          Length = 504

 Score =  579 bits (1492), Expect = 0.0
 Identities = 277/340 (81%), Positives = 310/340 (91%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGSS YG HWRNLRRITA ++LST+RIN+FA IR DE HRL++
Sbjct: 104  NRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTHRINTFADIRRDETHRLIR 163

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA D+S DF EVELTSKFYDMTFNNIMRMISGKRYYGDDCDM DVEEA QFRAMVS+LL
Sbjct: 164  KLAEDASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMADVEEARQFRAMVSELL 223

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            KLSGANNKNDF+P+LRL DYENLEKRL KI  K D FL GL+EE RSKK+  NTMVDHLL
Sbjct: 224  KLSGANNKNDFMPLLRLFDYENLEKRLKKISSKTDTFLNGLLEEQRSKKQRANTMVDHLL 283

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            SLQESQP+YYTD+IIKGL  G+L+AGTDSSAVTLEWALSCVL +PEVLK+TRDEL+T +G
Sbjct: 284  SLQESQPDYYTDEIIKGLALGMLVAGTDSSAVTLEWALSCVLKHPEVLKRTRDELETQVG 343

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            Q+RLLEESD+ K+PYLKNI++ETLRLYTPAPL LPHSSSEECII G+KVPRD+I+L NAW
Sbjct: 344  QERLLEESDISKLPYLKNIIFETLRLYTPAPLLLPHSSSEECIIGGFKVPRDSIVLINAW 403

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            +IHRDP+VWSEATSFKPER++ EGELEKLIAFGLGRRACP
Sbjct: 404  SIHRDPQVWSEATSFKPERYESEGELEKLIAFGLGRRACP 443


>ref|XP_017405614.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vigna angularis]
          Length = 500

 Score =  578 bits (1491), Expect = 0.0
 Identities = 276/340 (81%), Positives = 311/340 (91%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGSS YG HWRNLRRITA ++LST+RIN+FA IR DE HRL++
Sbjct: 100  NRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTHRINTFADIRRDETHRLIR 159

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA D+S DF EVELTSKFYDMTFNNIMRMISGKRYYGDDCDM DVEEA QFRAMVS+LL
Sbjct: 160  KLAEDASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMADVEEARQFRAMVSELL 219

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            KLSGANNKNDF+P+LRL D+ENLEKRL KI  K D FL GL+EE RS+K+  NTMVDHLL
Sbjct: 220  KLSGANNKNDFMPLLRLFDFENLEKRLKKISSKTDTFLNGLLEEQRSRKQGANTMVDHLL 279

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            SLQESQP+YYTDQIIKGL  G+L+AGTDSSAVTLEWALSC+LN+PEVLK+TRDEL+  +G
Sbjct: 280  SLQESQPDYYTDQIIKGLALGMLVAGTDSSAVTLEWALSCMLNHPEVLKRTRDELEKQVG 339

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            Q+RLLEESDLPK+PYLKNI++ETLRLYTPAPL LPHSSSEEC+I G+KVPRD+I+L NAW
Sbjct: 340  QERLLEESDLPKLPYLKNIIFETLRLYTPAPLLLPHSSSEECMIGGFKVPRDSIVLINAW 399

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            +IHRDP+VWSEATSFKPERF+ EGELEKLIAFG+GRRACP
Sbjct: 400  SIHRDPEVWSEATSFKPERFESEGELEKLIAFGMGRRACP 439


>gb|KOM25514.1| hypothetical protein LR48_Vigan107s003300 [Vigna angularis]
 dbj|BAT74198.1| hypothetical protein VIGAN_01181700 [Vigna angularis var. angularis]
          Length = 504

 Score =  578 bits (1491), Expect = 0.0
 Identities = 276/340 (81%), Positives = 311/340 (91%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGSS YG HWRNLRRITA ++LST+RIN+FA IR DE HRL++
Sbjct: 104  NRPRFLSGKYIFYNYTTLGSSPYGHHWRNLRRITALELLSTHRINTFADIRRDETHRLIR 163

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA D+S DF EVELTSKFYDMTFNNIMRMISGKRYYGDDCDM DVEEA QFRAMVS+LL
Sbjct: 164  KLAEDASSDFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMADVEEARQFRAMVSELL 223

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            KLSGANNKNDF+P+LRL D+ENLEKRL KI  K D FL GL+EE RS+K+  NTMVDHLL
Sbjct: 224  KLSGANNKNDFMPLLRLFDFENLEKRLKKISSKTDTFLNGLLEEQRSRKQGANTMVDHLL 283

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            SLQESQP+YYTDQIIKGL  G+L+AGTDSSAVTLEWALSC+LN+PEVLK+TRDEL+  +G
Sbjct: 284  SLQESQPDYYTDQIIKGLALGMLVAGTDSSAVTLEWALSCMLNHPEVLKRTRDELEKQVG 343

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            Q+RLLEESDLPK+PYLKNI++ETLRLYTPAPL LPHSSSEEC+I G+KVPRD+I+L NAW
Sbjct: 344  QERLLEESDLPKLPYLKNIIFETLRLYTPAPLLLPHSSSEECMIGGFKVPRDSIVLINAW 403

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            +IHRDP+VWSEATSFKPERF+ EGELEKLIAFG+GRRACP
Sbjct: 404  SIHRDPEVWSEATSFKPERFESEGELEKLIAFGMGRRACP 443


>ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
 gb|KRH42431.1| hypothetical protein GLYMA_08G089500 [Glycine max]
          Length = 502

 Score =  573 bits (1478), Expect = 0.0
 Identities = 279/344 (81%), Positives = 310/344 (90%), Gaps = 4/344 (1%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGK+IFYNYTTLGSS YGEHWRNLRRITA DVLST+R++SFA IR DE HRLV+
Sbjct: 98   NRPRFLSGKHIFYNYTTLGSSPYGEHWRNLRRITALDVLSTHRLHSFAAIRRDETHRLVR 157

Query: 842  KLA----ADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMV 675
            KLA    ++SS  F EVELTSKFYDMTFNNIMRMISGKRYYGDDCDM DVEEA QFRAMV
Sbjct: 158  KLAEAQGSESSLSFAEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMADVEEAKQFRAMV 217

Query: 674  SDLLKLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMV 495
            S+LLKL+GANNKNDF+P+LRL D+ENLEKRL KI +K D FLRGL+EE R+KK+  NTM+
Sbjct: 218  SELLKLAGANNKNDFMPVLRLFDFENLEKRLKKISNKTDTFLRGLLEEIRAKKQRANTML 277

Query: 494  DHLLSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELD 315
            DHLLSLQESQPEYYTDQIIKGL  G+L+A TDS AVTLEWALSCVLN+PEV K+ RDEL+
Sbjct: 278  DHLLSLQESQPEYYTDQIIKGLALGMLIAATDSQAVTLEWALSCVLNHPEVFKRARDELE 337

Query: 314  THIGQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIIL 135
            TH+GQD LLEESDL K+PYLKNI+YETLRLYTPAPL LPHSSSEECII G+KVP DTI+L
Sbjct: 338  THVGQDHLLEESDLSKLPYLKNIIYETLRLYTPAPLLLPHSSSEECIIGGFKVPGDTIVL 397

Query: 134  TNAWAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             NAW+IHRDPKVWSEATSFKPERF+KEGEL+KLIAFGLGRRACP
Sbjct: 398  INAWSIHRDPKVWSEATSFKPERFEKEGELDKLIAFGLGRRACP 441


>dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  553 bits (1424), Expect = 0.0
 Identities = 259/340 (76%), Positives = 309/340 (90%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRITA DVLS +RINSF+GIR DE  RL+ 
Sbjct: 98   NRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLIT 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            +LA DSS +F E+EL+ +FYDMTFNNIMRMISGKRYYG+DCDM+D++EA+QFR MVS+LL
Sbjct: 158  RLADDSSTNFAEIELSYRFYDMTFNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVSELL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            +LSGANNK DF+P+LR +D+ENLEKRL  I  K DAFLRGLI+EHR+KK+  NTM+DHLL
Sbjct: 218  QLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIQEHRAKKERANTMIDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            +LQ+SQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++S +LN+PEVLKK +DELDTH+G
Sbjct: 278  NLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRL++ESDLPK+ YLKN++ ETLRLYTPAPL LPHS+S+EC I GYKVP+DTI+L NAW
Sbjct: 338  QDRLVDESDLPKLSYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            AIHRDP++W+EAT+FKPERF+K+GELEKLIAFG+GRRACP
Sbjct: 398  AIHRDPELWTEATTFKPERFEKKGELEKLIAFGMGRRACP 437


>dbj|BAT21113.1| cytochrome P450 [synthetic construct]
          Length = 477

 Score =  551 bits (1419), Expect = 0.0
 Identities = 259/340 (76%), Positives = 308/340 (90%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRITA DVLS +RINSF+GIR DE  RL+ 
Sbjct: 76   NRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLIT 135

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            +LA DSS +F E+EL+S+ YDMTFNNIMRMISGKRYYG+DCD +D++EA+QFR MVS+LL
Sbjct: 136  RLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELL 195

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            +LSGANNK DF+P+LR +D+ENLEKRL  I  K DAFLRGLIEEHR+KK+  NTM+DHLL
Sbjct: 196  QLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLL 255

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            +LQ+SQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++S +LN+PEVLKK +DELDTH+G
Sbjct: 256  NLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVG 315

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRL++ESDLPK+ YLKN++ ETLRLYTPAPL LPHS+S+EC I GYKVP+DTI+L NAW
Sbjct: 316  QDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAW 375

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            AIHRDP++W+EAT+FKPERF+K+GELEKLIAFG+GRRACP
Sbjct: 376  AIHRDPELWTEATTFKPERFEKKGELEKLIAFGMGRRACP 415


>sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase; AltName: Full=CYP GE-3;
            AltName: Full=Cytochrome P450 81E1; AltName:
            Full=Cytochrome P450 91A4
 dbj|BAA22422.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  551 bits (1419), Expect = 0.0
 Identities = 259/340 (76%), Positives = 308/340 (90%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRITA DVLS +RINSF+GIR DE  RL+ 
Sbjct: 98   NRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLIT 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            +LA DSS +F E+EL+S+ YDMTFNNIMRMISGKRYYG+DCD +D++EA+QFR MVS+LL
Sbjct: 158  RLADDSSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            +LSGANNK DF+P+LR +D+ENLEKRL  I  K DAFLRGLIEEHR+KK+  NTM+DHLL
Sbjct: 218  QLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            +LQ+SQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++S +LN+PEVLKK +DELDTH+G
Sbjct: 278  NLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRL++ESDLPK+ YLKN++ ETLRLYTPAPL LPHS+S+EC I GYKVP+DTI+L NAW
Sbjct: 338  QDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            AIHRDP++W+EAT+FKPERF+K+GELEKLIAFG+GRRACP
Sbjct: 398  AIHRDPELWTEATTFKPERFEKKGELEKLIAFGMGRRACP 437


>ref|NP_001296643.1| isoflavone 2'-hydroxylase-like [Cicer arietinum]
 emb|CAB41490.1| cytochrome P450 monooxygenase [Cicer arietinum]
          Length = 498

 Score =  550 bits (1416), Expect = 0.0
 Identities = 256/340 (75%), Positives = 305/340 (89%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGS+SY EHWRNLRRIT+ DVLS +RIN+F+G+R DE  RL+ 
Sbjct: 98   NRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRINNFSGVRRDETQRLIT 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA DSS  F EVEL+ +FYDMTFNNIMRMISGKRYYG+DCDM+D++EA+QFR MV++LL
Sbjct: 158  KLAEDSSTSFAEVELSFRFYDMTFNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVTELL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            +LSGANNK DF+P+L+  D+ENLEKR+  I DK DAFLRGL++E R+KK+  NTM+DHLL
Sbjct: 218  QLSGANNKTDFMPLLKFFDFENLEKRVKNIADKTDAFLRGLLQEQRNKKERTNTMIDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            +LQESQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++S +LN+PEVLKK RDELDTH+G
Sbjct: 278  NLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVRDELDTHVG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRL++ESDLPK+ YLKN++YETLRL TPAPL LPHS+S++CII GYKVPRDTI+L NAW
Sbjct: 338  QDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCIIGGYKVPRDTIVLINAW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            AIHRDPK WSEAT+FKPERFDK+GE+EK+IAFG+GRR CP
Sbjct: 398  AIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCP 437


>ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago truncatula]
 sp|Q6WNR0.1|C81E7_MEDTR RecName: Full=Isoflavone 2'-hydroxylase; AltName:
            Full=4'-methoxyisoflavone 2'-hydroxylase; AltName:
            Full=Cytochrome P450 81E7
 gb|AAQ20040.1| isoflavone 2'-hydroxylase [Medicago truncatula]
 gb|KEH31433.1| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 498

 Score =  548 bits (1413), Expect = 0.0
 Identities = 255/340 (75%), Positives = 306/340 (90%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRIT+ DVLS +RIN+FA IR DE  RL++
Sbjct: 98   NRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRINNFAPIRRDETQRLIK 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA DSS  F EVELT +F+DMTFNNIMRMISGKRYYGDDCD+++V+EA+QFR MVS+LL
Sbjct: 158  KLAEDSSTKFAEVELTFRFFDMTFNNIMRMISGKRYYGDDCDISEVQEASQFRDMVSELL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            +LSGANNK DF+P+L+ +D+ENLEKR+ +I +KND FL GL++E RSKK+  NTM+DHLL
Sbjct: 218  QLSGANNKTDFMPLLKFLDFENLEKRVKRIGEKNDVFLSGLLQEQRSKKERTNTMIDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            ++QESQPEYYTD IIKGL   +LLAGTDSSAVTLEW +S +LN PEVLKK RDE+DTH+G
Sbjct: 278  NMQESQPEYYTDTIIKGLCLAMLLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRL++ESDLPK+ YL+N++YETLRLYTPAPL LPHS+++ECI+ GYKVPRDTI+L NAW
Sbjct: 338  QDRLVDESDLPKLTYLRNVIYETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            AIHRDP+ WSEAT+FKPERFDK+GELEK+IAFG+GRRACP
Sbjct: 398  AIHRDPETWSEATTFKPERFDKKGELEKMIAFGMGRRACP 437


>ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum]
 emb|CAA10067.1| cytochrome P450 [Cicer arietinum]
          Length = 498

 Score =  545 bits (1404), Expect = 0.0
 Identities = 253/340 (74%), Positives = 303/340 (89%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGS+SY EHWRNLRRIT+ DVLS +RIN+F+G+R DE  RL+ 
Sbjct: 98   NRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRINNFSGVRRDETQRLIT 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA DSS  F EVEL+ +FYDMTFNNIMRMI GKRYYG+DCDM+D++EA+QFR MV++LL
Sbjct: 158  KLAEDSSTSFAEVELSFRFYDMTFNNIMRMIPGKRYYGEDCDMSDLQEASQFRDMVTELL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            +LSGANNK DF+P+L+  D+ENLEKR+  I DK DAFLRGL++E R+KK+  NTM+DHLL
Sbjct: 218  QLSGANNKTDFMPLLKFFDFENLEKRVKNIADKTDAFLRGLLQEQRNKKERTNTMIDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            +LQESQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++S +LN+PE+LKK +DELD H+G
Sbjct: 278  NLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSNLLNHPEILKKVKDELDIHVG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRL++ESDLPK+ YLKN++YETLRL TPAPL LPHS+S++CII GYKVPRDTI+L NAW
Sbjct: 338  QDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCIIGGYKVPRDTIVLINAW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            AIHRDPK WSEATSFKPERFDK+GE+EK+IAFG+GRR CP
Sbjct: 398  AIHRDPKSWSEATSFKPERFDKKGEIEKVIAFGMGRRVCP 437


>ref|XP_004504637.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
          Length = 511

 Score =  542 bits (1397), Expect = 0.0
 Identities = 250/340 (73%), Positives = 307/340 (90%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRPKF+SGKYIFYNYTTLGS+SYGEHWRNLRRIT+ DVLS +RIN+F+GIR DE  RL++
Sbjct: 98   NRPKFISGKYIFYNYTTLGSASYGEHWRNLRRITSLDVLSNHRINNFSGIRKDETQRLIK 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA DSS +F EVEL+ +FYDMTFNNIMRMISGKRYYG+DCDM+D++EA+QFR MV++LL
Sbjct: 158  KLAEDSSSNFAEVELSFRFYDMTFNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVTELL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            +LSGANNK DFLP+LR +D+ENLEK++  I +K DAFL GL++E R+KK+  NTM+DHLL
Sbjct: 218  QLSGANNKTDFLPLLRFLDFENLEKKVKNIGEKTDAFLSGLLQEQRNKKERTNTMIDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            S+QESQPEYYTDQIIKGLV  +LLAGTD++A+TLEW++S +LN+PEVLKK RDELDTH+G
Sbjct: 278  SMQESQPEYYTDQIIKGLVLAMLLAGTDTTALTLEWSMSNLLNHPEVLKKVRDELDTHVG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRL++ESDLP + Y+KN++YETLRLY PAPL +PHS+S++CII GYK+PRDTI+L NAW
Sbjct: 338  QDRLVDESDLPNLTYIKNVIYETLRLYGPAPLLIPHSTSDDCIIGGYKIPRDTIVLINAW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             I RDP+ WSEAT+FKPERF+K+GELEKLIAFG+GRRACP
Sbjct: 398  GIQRDPESWSEATTFKPERFNKKGELEKLIAFGMGRRACP 437


>ref|XP_004504638.1| PREDICTED: isoflavone 2'-hydroxylase-like [Cicer arietinum]
          Length = 498

 Score =  540 bits (1392), Expect = 0.0
 Identities = 250/340 (73%), Positives = 302/340 (88%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGS+SY EHWRNLRRIT+ DVLS +RIN+F+G+R DE  RL+ 
Sbjct: 98   NRPRFLSGKYIFYNYTTLGSTSYSEHWRNLRRITSLDVLSNHRINNFSGVRRDETQRLIT 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA DSS  F EVEL+ +FYDMTFNNIMRMISGKRYYG+DCDM+D++EA+QFR MV++LL
Sbjct: 158  KLAEDSSTSFAEVELSFRFYDMTFNNIMRMISGKRYYGEDCDMSDLQEASQFRDMVTELL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            +LSGANNK DFLP+L+  D+ENLEKR+  I +K D FLRGL++E R+KK+  NTM+DHLL
Sbjct: 218  QLSGANNKTDFLPLLKFFDFENLEKRVKNIANKTDTFLRGLLQEQRNKKERTNTMIDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            +LQESQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++  ++N+PE+LKK +DELD H+G
Sbjct: 278  NLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNLMNHPEILKKVKDELDIHVG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRL++ESDLPK+ YLKN++YETLRL TPAPL LPHS+S++CII GYKVPRDTI+L NAW
Sbjct: 338  QDRLVDESDLPKLTYLKNVIYETLRLCTPAPLLLPHSTSDDCIIGGYKVPRDTIVLINAW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            AIHRDPK WSEAT+FKPERFDK+GE+EK+IAFG+GRR CP
Sbjct: 398  AIHRDPKSWSEATTFKPERFDKKGEIEKVIAFGMGRRVCP 437


>dbj|BAT21114.1| cytochrome P450 [synthetic construct]
          Length = 477

 Score =  535 bits (1379), Expect = 0.0
 Identities = 254/341 (74%), Positives = 305/341 (89%), Gaps = 1/341 (0%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRIT+ DVLS +RINSF+ IR DE  RL++
Sbjct: 76   NRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRINSFSPIRRDETTRLIR 135

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA DS+++F EVELTS+F+DMTFNNIMRMISGKRYYG+DCDMT+++EA++FR MV++LL
Sbjct: 136  KLAEDSAKNFSEVELTSRFFDMTFNNIMRMISGKRYYGEDCDMTELQEASEFRDMVTELL 195

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHD-NTMVDHL 486
            +LSGANNK DF+PILRLVD+E LEKR+  I  K D FLRGL++EHR KK+   NTM+DHL
Sbjct: 196  QLSGANNKADFMPILRLVDFEGLEKRVKGISSKTDRFLRGLLQEHRDKKQRTANTMIDHL 255

Query: 485  LSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHI 306
            L+LQESQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++  VLN PEVL+K + ELDTH+
Sbjct: 256  LTLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNVLNYPEVLEKIKAELDTHV 315

Query: 305  GQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNA 126
            GQDRL++ESD+PK+ YLKN++ ETLRLYTPAPL LPHS+S++C I GYKVPRDTI+L NA
Sbjct: 316  GQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCTIGGYKVPRDTIVLINA 375

Query: 125  WAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            WA+HRDP++W+EAT+FKPERFDK+GELEKLI FGLGRRACP
Sbjct: 376  WALHRDPQLWTEATTFKPERFDKKGELEKLIPFGLGRRACP 416


>dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]
          Length = 499

 Score =  535 bits (1379), Expect = 0.0
 Identities = 254/341 (74%), Positives = 305/341 (89%), Gaps = 1/341 (0%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTTLGS+SYGEHWRNLRRIT+ DVLS +RINSF+ IR DE  RL++
Sbjct: 98   NRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITSLDVLSNHRINSFSPIRRDETTRLIR 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA DS+++F EVELTS+F+DMTFNNIMRMISGKRYYG+DCDMT+++EA++FR MV++LL
Sbjct: 158  KLAEDSAKNFSEVELTSRFFDMTFNNIMRMISGKRYYGEDCDMTELQEASEFRDMVTELL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHD-NTMVDHL 486
            +LSGANNK DF+PILRLVD+E LEKR+  I  K D FLRGL++EHR KK+   NTM+DHL
Sbjct: 218  QLSGANNKADFMPILRLVDFEGLEKRVKGISSKTDRFLRGLLQEHRDKKQRTANTMIDHL 277

Query: 485  LSLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHI 306
            L+LQESQPEYYTDQIIKGL   +LLAGTDSSAVTLEW++  VLN PEVL+K + ELDTH+
Sbjct: 278  LTLQESQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMCNVLNYPEVLEKIKAELDTHV 337

Query: 305  GQDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNA 126
            GQDRL++ESD+PK+ YLKN++ ETLRLYTPAPL LPHS+S++C I GYKVPRDTI+L NA
Sbjct: 338  GQDRLVDESDIPKLTYLKNVINETLRLYTPAPLLLPHSASDDCTIGGYKVPRDTIVLINA 397

Query: 125  WAIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            WA+HRDP++W+EAT+FKPERFDK+GELEKLI FGLGRRACP
Sbjct: 398  WALHRDPQLWTEATTFKPERFDKKGELEKLIPFGLGRRACP 438


>ref|XP_016191270.1| isoflavone 2'-hydroxylase [Arachis ipaensis]
          Length = 499

 Score =  528 bits (1360), Expect = 0.0
 Identities = 243/340 (71%), Positives = 298/340 (87%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTT+GSS YGEHWRNLRRIT+ DVLS +R+NSF+ IR DE  RL++
Sbjct: 98   NRPRFLSGKYIFYNYTTVGSSEYGEHWRNLRRITSLDVLSNHRLNSFSPIRRDETTRLIR 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KL+ DS+E+F +VEL+SKFYDMTFNNIMRMISGKRYYG+DCDM DVEEA QFR M+S++L
Sbjct: 158  KLSEDSTENFAKVELSSKFYDMTFNNIMRMISGKRYYGEDCDMADVEEAKQFRQMISEVL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            +LSGANNK DF+P+L ++D +NL+KRLV I  + D FL GL++EHR+K +H NTM+DHLL
Sbjct: 218  QLSGANNKTDFIPLLGMLDVDNLKKRLVTISGRTDKFLNGLLQEHRTKNQHQNTMIDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            SLQ+SQP+YYTDQIIKGL   +LLAGTDSSAVTLEW++  +LN+PE L + R+ELDTH+G
Sbjct: 278  SLQDSQPDYYTDQIIKGLGLAMLLAGTDSSAVTLEWSMCNLLNHPEALNRVREELDTHVG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDR+++ESD+PK  YL+N++ ETLRLYTPAPL LPHSS+E+C I GY +P+DTI+L N W
Sbjct: 338  QDRIVDESDIPKFTYLRNVINETLRLYTPAPLLLPHSSAEKCTIGGYNIPKDTIVLINGW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            AIHRDPK W EATSFKPERF+++GELEKLIAFGLGRRACP
Sbjct: 398  AIHRDPKNWDEATSFKPERFERKGELEKLIAFGLGRRACP 437


>ref|XP_015957959.1| isoflavone 2'-hydroxylase [Arachis duranensis]
          Length = 499

 Score =  528 bits (1360), Expect = 0.0
 Identities = 243/340 (71%), Positives = 298/340 (87%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRP+FLSGKYIFYNYTT+GSS YGEHWRNLRRIT+ DVLS +R+NSF+ IR DE  RL++
Sbjct: 98   NRPRFLSGKYIFYNYTTVGSSEYGEHWRNLRRITSLDVLSNHRLNSFSPIRRDETTRLIR 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KL+ DS+E+F +VEL+SKFYDMTFNNIMRMISGKRYYG+DCDM DVEEA  FR M+S++L
Sbjct: 158  KLSEDSTENFAKVELSSKFYDMTFNNIMRMISGKRYYGEDCDMADVEEAKHFRQMISEVL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            +LSGANNK DF+P+L ++D +NL+KRLV I  + D FL GL++EHR+K +H NTM+DHLL
Sbjct: 218  QLSGANNKTDFIPLLGMLDVDNLKKRLVTISGRTDKFLNGLLQEHRTKNQHQNTMIDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            SLQ+SQP+YYTDQIIKGL   +LLAGTDSSAVTLEW++  +LN+PE L + R+ELDTH+G
Sbjct: 278  SLQDSQPDYYTDQIIKGLGLAMLLAGTDSSAVTLEWSMCNLLNHPEALNRVREELDTHVG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDR+++ESD+PK  YL+N++ ETLRLYTPAPL LPHSS+E+C I GYK+P+DTI+L N W
Sbjct: 338  QDRIVDESDIPKFTYLRNVINETLRLYTPAPLLLPHSSAEKCTIGGYKIPKDTIVLINGW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
            AIHRDPK W EATSFKPERF+++GELEKLIAFGLGRRACP
Sbjct: 398  AIHRDPKNWDEATSFKPERFERKGELEKLIAFGLGRRACP 437


>gb|OIW14266.1| hypothetical protein TanjilG_21406 [Lupinus angustifolius]
          Length = 498

 Score =  481 bits (1238), Expect = e-166
 Identities = 228/340 (67%), Positives = 277/340 (81%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRPK LSGK+IFYNYTT+GS SYGEHWRNLRRI ATDVLS+ RI SF+GIR DE HRLVQ
Sbjct: 98   NRPKNLSGKHIFYNYTTVGSCSYGEHWRNLRRIVATDVLSSQRILSFSGIRKDETHRLVQ 157

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA DSS  F +VELTS F DMT+NN+MRMI+GKRYYG+D ++ DV+EA +FR  V++LL
Sbjct: 158  KLAKDSSSKFAQVELTSTFRDMTYNNMMRMIAGKRYYGEDSELKDVDEAKEFRETVTELL 217

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            KL+G +NK D+LP L+  D+E L K+L     + D F+  LI+E RSKK+ +++M+DHLL
Sbjct: 218  KLAGVSNKADYLPFLKWFDFEGLNKKLTTTHKRFDVFVSALIKEQRSKKQREDSMIDHLL 277

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            +LQE+QP+YYTDQ+IKGL   +L AGTDSS  TLEWA   +LNNPEVLKK +DELDTHIG
Sbjct: 278  TLQETQPDYYTDQVIKGLTLAMLFAGTDSSGATLEWAFCNLLNNPEVLKKAKDELDTHIG 337

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRLL ESDLPK+PYLK IV ETLRLY PAPL +PH SSE+  + G+ +P DT+++ N W
Sbjct: 338  QDRLLTESDLPKLPYLKYIVQETLRLYPPAPLLIPHVSSEDITVEGFNIPSDTLVIVNGW 397

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             + RDPK WS+ATSFKPERF+KEGE +KLIAFGLGRRACP
Sbjct: 398  GMQRDPKFWSDATSFKPERFEKEGEEKKLIAFGLGRRACP 437


>ref|XP_019439320.1| PREDICTED: isoflavone 2'-hydroxylase-like [Lupinus angustifolius]
          Length = 516

 Score =  481 bits (1238), Expect = e-165
 Identities = 228/340 (67%), Positives = 277/340 (81%)
 Frame = -1

Query: 1022 NRPKFLSGKYIFYNYTTLGSSSYGEHWRNLRRITATDVLSTNRINSFAGIRNDEIHRLVQ 843
            NRPK LSGK+IFYNYTT+GS SYGEHWRNLRRI ATDVLS+ RI SF+GIR DE HRLVQ
Sbjct: 116  NRPKNLSGKHIFYNYTTVGSCSYGEHWRNLRRIVATDVLSSQRILSFSGIRKDETHRLVQ 175

Query: 842  KLAADSSEDFVEVELTSKFYDMTFNNIMRMISGKRYYGDDCDMTDVEEATQFRAMVSDLL 663
            KLA DSS  F +VELTS F DMT+NN+MRMI+GKRYYG+D ++ DV+EA +FR  V++LL
Sbjct: 176  KLAKDSSSKFAQVELTSTFRDMTYNNMMRMIAGKRYYGEDSELKDVDEAKEFRETVTELL 235

Query: 662  KLSGANNKNDFLPILRLVDYENLEKRLVKIRDKNDAFLRGLIEEHRSKKKHDNTMVDHLL 483
            KL+G +NK D+LP L+  D+E L K+L     + D F+  LI+E RSKK+ +++M+DHLL
Sbjct: 236  KLAGVSNKADYLPFLKWFDFEGLNKKLTTTHKRFDVFVSALIKEQRSKKQREDSMIDHLL 295

Query: 482  SLQESQPEYYTDQIIKGLVQGILLAGTDSSAVTLEWALSCVLNNPEVLKKTRDELDTHIG 303
            +LQE+QP+YYTDQ+IKGL   +L AGTDSS  TLEWA   +LNNPEVLKK +DELDTHIG
Sbjct: 296  TLQETQPDYYTDQVIKGLTLAMLFAGTDSSGATLEWAFCNLLNNPEVLKKAKDELDTHIG 355

Query: 302  QDRLLEESDLPKIPYLKNIVYETLRLYTPAPLSLPHSSSEECIIAGYKVPRDTIILTNAW 123
            QDRLL ESDLPK+PYLK IV ETLRLY PAPL +PH SSE+  + G+ +P DT+++ N W
Sbjct: 356  QDRLLTESDLPKLPYLKYIVQETLRLYPPAPLLIPHVSSEDITVEGFNIPSDTLVIVNGW 415

Query: 122  AIHRDPKVWSEATSFKPERFDKEGELEKLIAFGLGRRACP 3
             + RDPK WS+ATSFKPERF+KEGE +KLIAFGLGRRACP
Sbjct: 416  GMQRDPKFWSDATSFKPERFEKEGEEKKLIAFGLGRRACP 455