BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00005308
         (780 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PNX95764.1| putative carboxylesterase 6-like protein [Trifoli...   447   e-156
dbj|GAU48392.1| hypothetical protein TSUD_118180 [Trifolium subt...   441   e-154
ref|XP_013458944.1| CXE carboxylesterase [Medicago truncatula] >...   438   e-152
ref|XP_004499770.1| PREDICTED: probable carboxylesterase 15 [Cic...   437   e-152
dbj|BAA85654.1| hsr203J homolog [Pisum sativum]                       434   e-151
dbj|GAU48393.1| hypothetical protein TSUD_118190 [Trifolium subt...   427   e-148
ref|XP_020229340.1| probable carboxylesterase 15 [Cajanus cajan]...   421   e-146
dbj|GAU48394.1| hypothetical protein TSUD_118200 [Trifolium subt...   419   e-145
ref|XP_003527517.1| PREDICTED: probable carboxylesterase 15 [Gly...   411   e-142
gb|PNX83873.1| putative carboxylesterase 6-like protein [Trifoli...   408   e-141
ref|XP_014498796.1| probable carboxylesterase 15 [Vigna radiata ...   405   e-140
ref|XP_007148812.1| hypothetical protein PHAVU_005G016400g [Phas...   399   e-137
ref|XP_017424257.1| PREDICTED: probable carboxylesterase 15 [Vig...   397   e-136
ref|XP_016171325.1| probable carboxylesterase 15 [Arachis ipaensis]   396   e-136
ref|XP_015937066.1| probable carboxylesterase 15 [Arachis durane...   395   e-136
ref|XP_019413132.1| PREDICTED: probable carboxylesterase 15 [Lup...   395   e-136
gb|ADF18551.1| HSR203J protein [Arachis hypogaea]                     393   e-135
ref|XP_012068726.1| probable carboxylesterase 17 [Jatropha curca...   391   e-134
gb|OMO49782.1| Alpha/beta hydrolase-3 [Corchorus olitorius]           390   e-134
ref|XP_002525545.1| PREDICTED: probable carboxylesterase 15 [Ric...   389   e-133

>gb|PNX95764.1| putative carboxylesterase 6-like protein [Trifolium pratense]
 gb|PNY00049.1| putative carboxylesterase 6-like protein [Trifolium pratense]
          Length = 340

 Score =  447 bits (1149), Expect = e-156
 Identities = 209/261 (80%), Positives = 233/261 (89%), Gaps = 5/261 (1%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTT- 187
           MVQEKK+V+EVSGWLRIYDDGSVDRTWTGPPEV FMI+ V PH+QFI+GVA RDVTTTT 
Sbjct: 1   MVQEKKVVDEVSGWLRIYDDGSVDRTWTGPPEVEFMIEPVAPHEQFIEGVATRDVTTTTT 60

Query: 188 ----GSNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTI 355
               GS  R RLYLP+KTPK++ KLPIL H HGGGFCISEP+WFMYY +Y QF +ST++I
Sbjct: 61  TKNDGSIHRARLYLPEKTPKDNKKLPILIHFHGGGFCISEPNWFMYYHVYTQFARSTRSI 120

Query: 356 CVSPFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGN 535
           CVSPFLRRAPEHRLPA  +D FA LQWLQSVA+GDA DPWLEEHGDF+++FLIGDSSGGN
Sbjct: 121 CVSPFLRRAPEHRLPAAFEDGFATLQWLQSVAKGDARDPWLEEHGDFNKIFLIGDSSGGN 180

Query: 536 LVHELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFAL 715
           LVHE+A+RAGSVDLSP+RLAG IPIHPGFVRS+RSRSE+EMPQSPFLTLDMLDKFLS AL
Sbjct: 181 LVHEVAARAGSVDLSPIRLAGAIPIHPGFVRSDRSRSEIEMPQSPFLTLDMLDKFLSLAL 240

Query: 716 PIGSNKDHPITCPMGSAAPPL 778
           P GSNKDHP TCPMG+ APPL
Sbjct: 241 PTGSNKDHPFTCPMGAGAPPL 261


>dbj|GAU48392.1| hypothetical protein TSUD_118180 [Trifolium subterraneum]
          Length = 338

 Score =  441 bits (1135), Expect = e-154
 Identities = 207/259 (79%), Positives = 230/259 (88%), Gaps = 3/259 (1%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTT- 187
           MVQEKKLV+EVSGWLRIYDDGSVDRTWTGPPEV FMI+ V PH+QFI+GVAIRDVTT T 
Sbjct: 1   MVQEKKLVDEVSGWLRIYDDGSVDRTWTGPPEVEFMIEPVAPHEQFIEGVAIRDVTTATI 60

Query: 188 --GSNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICV 361
             GS  R RLYLP+KTPK++ KLPIL HLHGGGFCI+EP+ FMYY +Y QFV+ T++ICV
Sbjct: 61  NDGSIHRARLYLPEKTPKDNEKLPILIHLHGGGFCITEPNLFMYYHVYTQFVRCTRSICV 120

Query: 362 SPFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLV 541
           SPFLRRAPEHRLPA I+D FA LQWLQSVA+GD  DPWLEEHGDFS++FLIGDSSG NLV
Sbjct: 121 SPFLRRAPEHRLPAAIEDGFATLQWLQSVAKGDTRDPWLEEHGDFSKIFLIGDSSGANLV 180

Query: 542 HELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPI 721
           HE+A RAGS+DLSP+RLAGGIPIHPGF RSNRS+SE+EMPQ+PFLTLDMLD FLS ALPI
Sbjct: 181 HEVAGRAGSIDLSPIRLAGGIPIHPGFARSNRSQSEIEMPQTPFLTLDMLDNFLSLALPI 240

Query: 722 GSNKDHPITCPMGSAAPPL 778
           GSNKDHP TCPMG  APPL
Sbjct: 241 GSNKDHPFTCPMGEGAPPL 259


>ref|XP_013458944.1| CXE carboxylesterase [Medicago truncatula]
 gb|KEH32986.1| CXE carboxylesterase [Medicago truncatula]
          Length = 343

 Score =  438 bits (1126), Expect = e-152
 Identities = 206/264 (78%), Positives = 231/264 (87%), Gaps = 8/264 (3%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTT- 187
           MVQ+KKLV+EVSGWLRIY+DGSVDRTWTGPPEV FMI+ V PH+QFI+GVAIRDVTTTT 
Sbjct: 1   MVQQKKLVDEVSGWLRIYNDGSVDRTWTGPPEVTFMIEPVAPHEQFIEGVAIRDVTTTTS 60

Query: 188 -------GSNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQST 346
                  GS  R RLYLP+KTP+++ KLPIL H  GGGFCISEP+WFMYY +Y + V+ST
Sbjct: 61  TTTNTQDGSIHRARLYLPEKTPEDNKKLPILLHFQGGGFCISEPNWFMYYHVYTELVRST 120

Query: 347 QTICVSPFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSS 526
           ++ICVSP+LRRAPEHRLPA IDD FA LQWLQSVA+GD  DPWLEEHGDF ++FLIGDSS
Sbjct: 121 RSICVSPYLRRAPEHRLPAAIDDGFATLQWLQSVAKGDVRDPWLEEHGDFDKIFLIGDSS 180

Query: 527 GGNLVHELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLS 706
           GGN+VHE+A+RAGSVDL+PVRLAG IPIHPGFVRS RSRSE+EMPQSPFLTLDMLDKFL 
Sbjct: 181 GGNIVHEVAARAGSVDLTPVRLAGAIPIHPGFVRSYRSRSEIEMPQSPFLTLDMLDKFLG 240

Query: 707 FALPIGSNKDHPITCPMGSAAPPL 778
            ALPIGSNKDHP TCPMGS APPL
Sbjct: 241 LALPIGSNKDHPFTCPMGSGAPPL 264


>ref|XP_004499770.1| PREDICTED: probable carboxylesterase 15 [Cicer arietinum]
          Length = 338

 Score =  437 bits (1125), Expect = e-152
 Identities = 204/259 (78%), Positives = 230/259 (88%), Gaps = 3/259 (1%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTT- 187
           MVQEKK+V+EVSGW RIY+DGSV+R+WTGPPEVNFM++ V PH QFI+ VA RD+TTTT 
Sbjct: 1   MVQEKKVVDEVSGWFRIYNDGSVERSWTGPPEVNFMLEPVSPHTQFIEAVATRDITTTTT 60

Query: 188 --GSNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICV 361
             G   R R+YLP+KTPK+++KLPIL H HGGGFCISEPDWFMYY +Y +FV+ST++ICV
Sbjct: 61  HDGLIHRARIYLPEKTPKDNHKLPILIHFHGGGFCISEPDWFMYYHVYTRFVRSTRSICV 120

Query: 362 SPFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLV 541
           SPFLRRAPEHRLPA IDD F  LQWLQSVARGD +DPWLEEHGDFSRVFLIGDSSGGNLV
Sbjct: 121 SPFLRRAPEHRLPAAIDDGFTTLQWLQSVARGDVSDPWLEEHGDFSRVFLIGDSSGGNLV 180

Query: 542 HELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPI 721
           HE+A+R GS+DLSP+ LAG IPIHPGFVRS RS+SELEMPQSPFLTLDM+DKFL  ALPI
Sbjct: 181 HEVAARVGSIDLSPIWLAGAIPIHPGFVRSYRSKSELEMPQSPFLTLDMMDKFLRMALPI 240

Query: 722 GSNKDHPITCPMGSAAPPL 778
           GSNKDHPITCPMG AAPPL
Sbjct: 241 GSNKDHPITCPMGEAAPPL 259


>dbj|BAA85654.1| hsr203J homolog [Pisum sativum]
          Length = 339

 Score =  434 bits (1115), Expect = e-151
 Identities = 205/260 (78%), Positives = 230/260 (88%), Gaps = 4/260 (1%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MVQEKKLV+EVSGWLRIYDDGSVDRTWTGPPEVNFMI+ V PH++FIDGVA RDVT +T 
Sbjct: 1   MVQEKKLVDEVSGWLRIYDDGSVDRTWTGPPEVNFMIEPVAPHEEFIDGVATRDVTMSTT 60

Query: 191 SNQ----RVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTIC 358
           +N     R RLYLP+KTP  + KLPIL H HGGGFCI+EPD FMYY +Y +FV+ST++IC
Sbjct: 61  TNDNFIHRARLYLPEKTPTENEKLPILIHFHGGGFCITEPDCFMYYKVYTRFVKSTRSIC 120

Query: 359 VSPFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNL 538
           VSPFLRRAPEHRLPA I+D FA L+WLQSVA+GDA+DPWLE+HGDF+RVFLIGDSSGGNL
Sbjct: 121 VSPFLRRAPEHRLPAAIEDGFATLRWLQSVAKGDAHDPWLEKHGDFNRVFLIGDSSGGNL 180

Query: 539 VHELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALP 718
           VHE+++RA S DL PVRLAG IPIHPG+VRS RSRSE EMPQSPFLTLDMLDKFLS +LP
Sbjct: 181 VHEVSARASSTDLRPVRLAGAIPIHPGYVRSERSRSENEMPQSPFLTLDMLDKFLSLSLP 240

Query: 719 IGSNKDHPITCPMGSAAPPL 778
           IGSNKDHPITCPMG AAPPL
Sbjct: 241 IGSNKDHPITCPMGEAAPPL 260


>dbj|GAU48393.1| hypothetical protein TSUD_118190 [Trifolium subterraneum]
          Length = 340

 Score =  427 bits (1097), Expect = e-148
 Identities = 203/261 (77%), Positives = 227/261 (86%), Gaps = 5/261 (1%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTT- 187
           MVQEKKLV+EVSGWLRIYDDGSVDRTWTGPPEV FMI+ V PH+ FI GVAIRDVTT+  
Sbjct: 1   MVQEKKLVDEVSGWLRIYDDGSVDRTWTGPPEVKFMIEPVAPHEHFIKGVAIRDVTTSMT 60

Query: 188 ----GSNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTI 355
               GS  R RLYLP+KTP ++ K+PIL H HGGGFCI+EP+ FMYY +Y QFV+ T++I
Sbjct: 61  TTNDGSIHRARLYLPEKTPNDNIKMPILMHFHGGGFCITEPNSFMYYHVYTQFVRCTRSI 120

Query: 356 CVSPFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGN 535
           CVSPFLR+APEHRLPA IDD FA L+WLQSVARGDA DPWLEE+GDF +VFLIGDS+GGN
Sbjct: 121 CVSPFLRQAPEHRLPAAIDDGFATLKWLQSVARGDARDPWLEEYGDFDKVFLIGDSAGGN 180

Query: 536 LVHELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFAL 715
           LVHE+A+RAGS DLSPVRLAG IPIHPGFVRS RS SE+EMPQSPFLTLDMLDKFLS AL
Sbjct: 181 LVHEIAARAGSTDLSPVRLAGAIPIHPGFVRSIRSNSEIEMPQSPFLTLDMLDKFLSLAL 240

Query: 716 PIGSNKDHPITCPMGSAAPPL 778
           PIGSNKDHP TCPMG+ APP+
Sbjct: 241 PIGSNKDHPFTCPMGTTAPPI 261


>ref|XP_020229340.1| probable carboxylesterase 15 [Cajanus cajan]
 gb|KYP53492.1| putative gibberellin receptor GID1L3 [Cajanus cajan]
          Length = 335

 Score =  421 bits (1082), Expect = e-146
 Identities = 191/256 (74%), Positives = 225/256 (87%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MVQEKKLV+EVSGWL+IYDDGSV+RTW+GP +  FM + VPPH+QF+DGVA RDV    G
Sbjct: 1   MVQEKKLVDEVSGWLKIYDDGSVNRTWSGPDQFKFMAEPVPPHEQFVDGVATRDVIAHDG 60

Query: 191 SNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVSPF 370
           S  RVRLYLP+K PK++ KLPIL H HGGGFCISEPDWFMYY++Y +F +ST++I VSPF
Sbjct: 61  SGHRVRLYLPEKAPKDNQKLPILLHFHGGGFCISEPDWFMYYNVYTRFARSTRSIVVSPF 120

Query: 371 LRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVHEL 550
           LRR+PEHRLPA IDDAF  L WLQSVAR  + +PWLE+H DF RVFLIGDSSGGN+VHE+
Sbjct: 121 LRRSPEHRLPAAIDDAFETLLWLQSVARSGSREPWLEQHADFDRVFLIGDSSGGNVVHEV 180

Query: 551 ASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIGSN 730
           A+RAGSVD+SPVR+AG IP+HPG+VRSNRSRSE+EMPQ+PFLTLDMLDKFL+ ALP+G+ 
Sbjct: 181 AARAGSVDMSPVRVAGAIPVHPGYVRSNRSRSEMEMPQTPFLTLDMLDKFLTLALPVGAT 240

Query: 731 KDHPITCPMGSAAPPL 778
           KDHPITCPMG+AAPPL
Sbjct: 241 KDHPITCPMGAAAPPL 256


>dbj|GAU48394.1| hypothetical protein TSUD_118200 [Trifolium subterraneum]
          Length = 340

 Score =  419 bits (1078), Expect = e-145
 Identities = 200/261 (76%), Positives = 223/261 (85%), Gaps = 5/261 (1%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTT- 187
           M+QEKKLV+EVSGWLRIYDDGSVDRTWTGPPE  FMI  V PH+QFI+GVA RDVTT T 
Sbjct: 1   MIQEKKLVDEVSGWLRIYDDGSVDRTWTGPPEAKFMIDPVAPHEQFIEGVATRDVTTATI 60

Query: 188 ----GSNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTI 355
               GS  R RLYLP+KT K++ KLPI+ H HGGGFCI+E + +MYY +Y +FV+ T++I
Sbjct: 61  TANDGSIHRARLYLPEKTTKDNKKLPIVIHFHGGGFCITETNSYMYYHVYTKFVRCTRSI 120

Query: 356 CVSPFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGN 535
           CVSPFLR+APEHRLPA IDD FA LQWLQSVARGD  D WLEEHGDF++VFLIGDSSGGN
Sbjct: 121 CVSPFLRQAPEHRLPAAIDDGFATLQWLQSVARGDVCDSWLEEHGDFNKVFLIGDSSGGN 180

Query: 536 LVHELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFAL 715
           LVHE+A+RAG  DLSPVRLAG IPIHPGFVRS RS SE+EMPQSPFLTLDMLD FLS AL
Sbjct: 181 LVHEVAARAGLADLSPVRLAGAIPIHPGFVRSTRSNSEIEMPQSPFLTLDMLDNFLSLAL 240

Query: 716 PIGSNKDHPITCPMGSAAPPL 778
           PIGSNKDHP TCPMG+AAPPL
Sbjct: 241 PIGSNKDHPFTCPMGAAAPPL 261


>ref|XP_003527517.1| PREDICTED: probable carboxylesterase 15 [Glycine max]
 gb|KRH56206.1| hypothetical protein GLYMA_06G310100 [Glycine max]
          Length = 338

 Score =  411 bits (1056), Expect = e-142
 Identities = 191/259 (73%), Positives = 220/259 (84%), Gaps = 3/259 (1%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MVQEKKLV+EVSGWL+IYDDGSVDRTW+GP +  FM +  PPH+QFIDGVAIRDV  T G
Sbjct: 1   MVQEKKLVDEVSGWLKIYDDGSVDRTWSGPDQFKFMAEPAPPHEQFIDGVAIRDVAVTHG 60

Query: 191 SNQ---RVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICV 361
             Q    VRLYLP+  P++S KLPI+ H HGGGFCISEPDWFMYY +Y +F +ST++I V
Sbjct: 61  GGQSGHHVRLYLPEIKPEDSQKLPIVLHFHGGGFCISEPDWFMYYQVYTRFARSTRSIVV 120

Query: 362 SPFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLV 541
           SPFLRRAPEHRLPA IDD F  L WLQ+VAR  + +PWLE+HGDF+RVFLIGDSSGGN V
Sbjct: 121 SPFLRRAPEHRLPAAIDDGFDTLLWLQTVARSGSLEPWLEQHGDFNRVFLIGDSSGGNSV 180

Query: 542 HELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPI 721
           HE+A+RAGS DLSPVR+AG IP+HPGFVRSNRSRSE+EMPQ+PFLTLDMLDKFL+ ALP+
Sbjct: 181 HEVAARAGSADLSPVRVAGAIPVHPGFVRSNRSRSEMEMPQTPFLTLDMLDKFLALALPV 240

Query: 722 GSNKDHPITCPMGSAAPPL 778
           G+ KDHP TCPMG AAPPL
Sbjct: 241 GATKDHPFTCPMGEAAPPL 259


>gb|PNX83873.1| putative carboxylesterase 6-like protein [Trifolium pratense]
          Length = 340

 Score =  408 bits (1048), Expect = e-141
 Identities = 194/261 (74%), Positives = 220/261 (84%), Gaps = 5/261 (1%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTT- 187
           MVQEKKLVE+V GWLRIYDDGSVDRTW GPPE  FMI  V PH+QFI+GVA RDVTT   
Sbjct: 1   MVQEKKLVEDVYGWLRIYDDGSVDRTWRGPPEAKFMIDPVAPHEQFIEGVATRDVTTAMI 60

Query: 188 ----GSNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTI 355
               GS  R RLYLP+KT K++ KLPI+ H HGGGFCI+E + +MYY +Y +FV+ T++I
Sbjct: 61  TTNEGSIHRARLYLPEKTTKDNKKLPIVIHFHGGGFCITEANSYMYYHVYTKFVRCTRSI 120

Query: 356 CVSPFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGN 535
           CVSPFLR+APEHRLPA IDD  A LQWLQ VARGDA DPWLEEHGDF++VFLIGDS+GGN
Sbjct: 121 CVSPFLRQAPEHRLPAAIDDGLATLQWLQLVARGDACDPWLEEHGDFNKVFLIGDSTGGN 180

Query: 536 LVHELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFAL 715
           LVHE+A+R GSVDLSPVRL G IPIHPGFVRS RSRSE+E+PQSPF TLDMLDK L+ AL
Sbjct: 181 LVHEVAARVGSVDLSPVRLVGAIPIHPGFVRSVRSRSEIELPQSPFQTLDMLDKLLNLAL 240

Query: 716 PIGSNKDHPITCPMGSAAPPL 778
           P+GSNKDHP TCPMG+AAPPL
Sbjct: 241 PVGSNKDHPFTCPMGAAAPPL 261


>ref|XP_014498796.1| probable carboxylesterase 15 [Vigna radiata var. radiata]
          Length = 336

 Score =  405 bits (1041), Expect = e-140
 Identities = 190/257 (73%), Positives = 217/257 (84%), Gaps = 1/257 (0%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRD-VTTTT 187
           MVQ+KKLV+EVSGWLRIYDDGSVDRTWTGP +  F+   VP H++FIDG+A+RD + +  
Sbjct: 1   MVQQKKLVDEVSGWLRIYDDGSVDRTWTGPAQFKFLADPVPSHEKFIDGIAVRDTIVSHE 60

Query: 188 GSNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVSP 367
           G + RVRLYLP+K  +N  KLPI+ H  GGGFCISEPDWFMYY IY +   STQ I VSP
Sbjct: 61  GHDLRVRLYLPEKALENKKKLPIILHFQGGGFCISEPDWFMYYQIYVRIALSTQAIVVSP 120

Query: 368 FLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVHE 547
           FLRRAPEHRLP  IDDAF  L WL+SVAR  + +PWLE+HGDF+RVFLIGDSSGGN+VHE
Sbjct: 121 FLRRAPEHRLPTAIDDAFDTLLWLRSVARSGSREPWLEQHGDFNRVFLIGDSSGGNVVHE 180

Query: 548 LASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIGS 727
           +ASRAGSVDLSPVR+AG IP+HPGFVRS RSRSELEMPQSPFLTLDMLDKFL  ALP+G+
Sbjct: 181 VASRAGSVDLSPVRVAGAIPVHPGFVRSERSRSELEMPQSPFLTLDMLDKFLELALPVGA 240

Query: 728 NKDHPITCPMGSAAPPL 778
            KDHPITCPMG+AAPPL
Sbjct: 241 TKDHPITCPMGAAAPPL 257


>ref|XP_007148812.1| hypothetical protein PHAVU_005G016400g [Phaseolus vulgaris]
 gb|ESW20806.1| hypothetical protein PHAVU_005G016400g [Phaseolus vulgaris]
          Length = 336

 Score =  399 bits (1024), Expect = e-137
 Identities = 187/257 (72%), Positives = 213/257 (82%), Gaps = 1/257 (0%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MV EKKLV+EVSGWL+IYDDGSVDRTWTGPP+  F+   V PH QFIDGVA+RD T + G
Sbjct: 1   MVHEKKLVDEVSGWLKIYDDGSVDRTWTGPPQFKFLSDPVLPHQQFIDGVAVRDTTISLG 60

Query: 191 S-NQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVSP 367
             N RVRLYLP+   ++  KLPI+ H HGGGFCISEPDWFMYY  Y +   + Q I VSP
Sbjct: 61  DHNLRVRLYLPEVKSESKKKLPIILHFHGGGFCISEPDWFMYYHFYGRVALAAQAIVVSP 120

Query: 368 FLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVHE 547
           FLRRAPEHRLPA IDDAF  L WLQ+VA+  + +PWLE+HGDF+RVFLIGDSSGGN+VHE
Sbjct: 121 FLRRAPEHRLPAAIDDAFDTLLWLQAVAQSGSQEPWLEQHGDFNRVFLIGDSSGGNVVHE 180

Query: 548 LASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIGS 727
           +ASRAGSV+L PVR+AG IP+HPG+VRS RSRSELEMPQSPFLTLDMLDKFL  ALP+G+
Sbjct: 181 VASRAGSVELRPVRVAGAIPVHPGYVRSERSRSELEMPQSPFLTLDMLDKFLELALPVGA 240

Query: 728 NKDHPITCPMGSAAPPL 778
            KDHPITCPMG AAPPL
Sbjct: 241 TKDHPITCPMGEAAPPL 257


>ref|XP_017424257.1| PREDICTED: probable carboxylesterase 15 [Vigna angularis]
 gb|KOM43149.1| hypothetical protein LR48_Vigan05g075300 [Vigna angularis]
 dbj|BAT92787.1| hypothetical protein VIGAN_07162400 [Vigna angularis var.
           angularis]
          Length = 336

 Score =  397 bits (1020), Expect = e-136
 Identities = 187/257 (72%), Positives = 214/257 (83%), Gaps = 1/257 (0%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRD-VTTTT 187
           MVQ+K LV+EVSGWLRIYDDGSVDRTWTGP +  FM   VP H++FIDG+A+RD + +  
Sbjct: 1   MVQQKNLVDEVSGWLRIYDDGSVDRTWTGPAQFKFMSDPVPSHEKFIDGIAVRDTIISRE 60

Query: 188 GSNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVSP 367
           G + RVRLYLP+K  ++  KLPI+ H  GGGFCISEPDWFMYY IY +   STQ I VSP
Sbjct: 61  GHDLRVRLYLPEKALESKKKLPIILHFQGGGFCISEPDWFMYYQIYVRVALSTQAIVVSP 120

Query: 368 FLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVHE 547
           FLRRAPEHRLP  IDDAF  L WLQSVAR  + +PWLE+HGDF+R+FLIGDSSGGN+VHE
Sbjct: 121 FLRRAPEHRLPTAIDDAFDTLLWLQSVARSGSREPWLEQHGDFNRLFLIGDSSGGNVVHE 180

Query: 548 LASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIGS 727
           +ASRAGSVDLSPVR+AG IP+HPGFVRS RSRSEL+MPQSPFLTLDMLDKFL  ALP+G+
Sbjct: 181 VASRAGSVDLSPVRVAGAIPVHPGFVRSERSRSELKMPQSPFLTLDMLDKFLELALPVGA 240

Query: 728 NKDHPITCPMGSAAPPL 778
            KDHPITCPMG AAP L
Sbjct: 241 TKDHPITCPMGEAAPAL 257


>ref|XP_016171325.1| probable carboxylesterase 15 [Arachis ipaensis]
          Length = 334

 Score =  396 bits (1018), Expect = e-136
 Identities = 183/256 (71%), Positives = 214/256 (83%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MVQEKKLVE VSGWLRIYDDGSVDRTWTGPPE  FM + VPPH++FIDGVA RD+T    
Sbjct: 1   MVQEKKLVENVSGWLRIYDDGSVDRTWTGPPEAKFMAEPVPPHEEFIDGVATRDITVAE- 59

Query: 191 SNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVSPF 370
           SN+ VRLYLP+ T  +  KLP++ H  GGGFCISEPDWFMYY++Y +F ++ + ICVSPF
Sbjct: 60  SNRSVRLYLPEITSADKEKLPVVVHFQGGGFCISEPDWFMYYNMYTRFARAARFICVSPF 119

Query: 371 LRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVHEL 550
           LRRAPEHRLPA I+D F+ L WLQSVA+G++ + WLE+H DFSRVFLIGDSSGGN+VHE+
Sbjct: 120 LRRAPEHRLPAAIEDGFSTLLWLQSVAKGESKELWLEKHADFSRVFLIGDSSGGNVVHEV 179

Query: 551 ASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIGSN 730
           A+ AG   L P+RLAG IP+HPGF+RS RS+SELE PQSPFLTLDMLD FL+ ALP+GS 
Sbjct: 180 AALAGKASLKPLRLAGAIPVHPGFLRSTRSKSELEKPQSPFLTLDMLDNFLALALPVGST 239

Query: 731 KDHPITCPMGSAAPPL 778
           KDHPITCPMG AAPPL
Sbjct: 240 KDHPITCPMGEAAPPL 255


>ref|XP_015937066.1| probable carboxylesterase 15 [Arachis duranensis]
          Length = 334

 Score =  395 bits (1015), Expect = e-136
 Identities = 182/256 (71%), Positives = 214/256 (83%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MVQEKKLV+ VSGWLRIYDDGSVDRTWTGPPE  FM + VPPH++FIDGVA RD+T    
Sbjct: 1   MVQEKKLVQNVSGWLRIYDDGSVDRTWTGPPEAKFMAEPVPPHEEFIDGVATRDITVAE- 59

Query: 191 SNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVSPF 370
           SN+ VRLYLP+ T  +  KLP++ H  GGGFCISEPDWFMYY++Y +F ++ + ICVSPF
Sbjct: 60  SNRSVRLYLPEITSADKEKLPVVVHFQGGGFCISEPDWFMYYNMYTRFARAARFICVSPF 119

Query: 371 LRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVHEL 550
           LRRAPEHRLPA I+D F+ L WLQSVA+G++ + WLE+H DFSRVFLIGDSSGGN+VHE+
Sbjct: 120 LRRAPEHRLPAAIEDGFSTLLWLQSVAKGESKELWLEKHADFSRVFLIGDSSGGNVVHEV 179

Query: 551 ASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIGSN 730
           A+ AG   L P+RLAG IP+HPGF+RS RS+SELE PQSPFLTLDMLD FL+ ALP+GS 
Sbjct: 180 AALAGKASLEPLRLAGAIPVHPGFLRSTRSKSELEKPQSPFLTLDMLDNFLALALPVGST 239

Query: 731 KDHPITCPMGSAAPPL 778
           KDHPITCPMG AAPPL
Sbjct: 240 KDHPITCPMGEAAPPL 255


>ref|XP_019413132.1| PREDICTED: probable carboxylesterase 15 [Lupinus angustifolius]
 gb|OIV99073.1| hypothetical protein TanjilG_32332 [Lupinus angustifolius]
          Length = 337

 Score =  395 bits (1015), Expect = e-136
 Identities = 184/258 (71%), Positives = 211/258 (81%), Gaps = 2/258 (0%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MVQEKKLVE+VSGWLR+YDDGSVDRTWTGPP+V FM++ VPPH  F DGVA RDV   + 
Sbjct: 1   MVQEKKLVEDVSGWLRLYDDGSVDRTWTGPPQVKFMVQPVPPHQHFKDGVATRDVIIGSQ 60

Query: 191 SN--QRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVS 364
            N   R+RLYLP+        LPI+ H HGGGFCISE DWFMYY  Y +F +S + ICVS
Sbjct: 61  DNTDHRLRLYLPEIKSDEKEALPIVLHFHGGGFCISEADWFMYYQFYTRFTRSARVICVS 120

Query: 365 PFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVH 544
           PFLR APE+RLPA IDD F+AL WLQ VA GD  +PWL+EHGDF+RVFLIGDSSGGN+VH
Sbjct: 121 PFLRHAPENRLPAGIDDGFSALLWLQKVATGDVEEPWLKEHGDFNRVFLIGDSSGGNIVH 180

Query: 545 ELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIG 724
           E+A+RAG++ L P+RLAGGIP+HPGF+RS RSRSELE PQSPFLTLDM+DKFL  ALP+G
Sbjct: 181 EVAARAGNIKLDPLRLAGGIPVHPGFLRSTRSRSELEKPQSPFLTLDMVDKFLGLALPLG 240

Query: 725 SNKDHPITCPMGSAAPPL 778
           SNKDHPITCPMG  APPL
Sbjct: 241 SNKDHPITCPMGDNAPPL 258


>gb|ADF18551.1| HSR203J protein [Arachis hypogaea]
          Length = 335

 Score =  393 bits (1009), Expect = e-135
 Identities = 181/256 (70%), Positives = 211/256 (82%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MVQEKKLV+ VSGWLRIYDDGSVDRTWTGPPE  FM + VPPH++FIDGVA RD+ T   
Sbjct: 1   MVQEKKLVQNVSGWLRIYDDGSVDRTWTGPPEAKFMAEPVPPHEEFIDGVATRDIITVAE 60

Query: 191 SNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVSPF 370
           SN+ VRLYLP        KLP++ H  GGGFCISEPDWFMYY++Y +F ++ + ICVSPF
Sbjct: 61  SNRSVRLYLPGDYICCKEKLPVVVHFQGGGFCISEPDWFMYYNMYTRFARAARFICVSPF 120

Query: 371 LRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVHEL 550
           LRRAPEHRLPA I+D F+ L WLQSVA+G++ + WLE+H DFSRVFLIGDSSGGN+VHE+
Sbjct: 121 LRRAPEHRLPAAIEDGFSTLLWLQSVAKGESKELWLEKHADFSRVFLIGDSSGGNVVHEV 180

Query: 551 ASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIGSN 730
           A+ AG   L P+RLAG IP+HPGF+RS RS+SELE PQSPFLTLDMLD FL+ ALP+GS 
Sbjct: 181 AALAGKASLKPLRLAGAIPVHPGFLRSTRSKSELEKPQSPFLTLDMLDNFLALALPVGST 240

Query: 731 KDHPITCPMGSAAPPL 778
           KDHPITCPMG AAPPL
Sbjct: 241 KDHPITCPMGEAAPPL 256


>ref|XP_012068726.1| probable carboxylesterase 17 [Jatropha curcas]
 gb|KDP40570.1| hypothetical protein JCGZ_24569 [Jatropha curcas]
          Length = 336

 Score =  391 bits (1004), Expect = e-134
 Identities = 181/257 (70%), Positives = 212/257 (82%), Gaps = 1/257 (0%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MV E+K+VEEVSGWLR+YDDGSVDRTWTGPP+V FM + VP H+QFIDGVA+RDVT  + 
Sbjct: 1   MVHERKIVEEVSGWLRVYDDGSVDRTWTGPPQVKFMAEPVPAHEQFIDGVAVRDVTVNSN 60

Query: 191 SNQRVRLYLPKKT-PKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVSP 367
           S  RVR+YLP+     N NKLPI+ H HGGGFCIS+ DW+MYY IY +  +S + ICVS 
Sbjct: 61  SGLRVRIYLPETNLESNDNKLPIILHFHGGGFCISQADWYMYYHIYTRLARSAKAICVSV 120

Query: 368 FLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVHE 547
           FLR APEHRLPA IDD F+AL WL S+A G + + WL  HG+F+RVFLIGDSSGGN+VHE
Sbjct: 121 FLRLAPEHRLPAAIDDGFSALLWLSSLANGKSQESWLNNHGNFNRVFLIGDSSGGNVVHE 180

Query: 548 LASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIGS 727
           +A+RAG +DLSP+RLAG IPIHPGFVRS RS+SELE P+SPFLTLDM+DKFL  ALPIG 
Sbjct: 181 VAARAGHMDLSPLRLAGAIPIHPGFVRSERSKSELEQPESPFLTLDMVDKFLGLALPIGC 240

Query: 728 NKDHPITCPMGSAAPPL 778
           NKDHPITCPMG+AAP L
Sbjct: 241 NKDHPITCPMGAAAPKL 257


>gb|OMO49782.1| Alpha/beta hydrolase-3 [Corchorus olitorius]
          Length = 335

 Score =  390 bits (1001), Expect = e-134
 Identities = 174/256 (67%), Positives = 214/256 (83%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MVQEKK+V+EVSGWL +YDDGSVDR WTGPPEV FM + VPPH+ F+ GVA RDVT  + 
Sbjct: 1   MVQEKKIVDEVSGWLTVYDDGSVDRAWTGPPEVKFMTEPVPPHENFVQGVATRDVTIDSN 60

Query: 191 SNQRVRLYLPKKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVSPF 370
           S  +VR+YLP++ P  + KLPI+ H HGGGFCIS+ DWFMYYSIY +  +S   ICVS +
Sbjct: 61  SGLKVRIYLPEQIPTTNTKLPIILHFHGGGFCISQADWFMYYSIYTRLARSVPAICVSVY 120

Query: 371 LRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVHEL 550
           LR APE++LPA  DD +AAL WL+S+A+G++++PWL EH DF+RVFLIGDSSGGN+VH++
Sbjct: 121 LRLAPENKLPAACDDGYAALLWLRSLAKGESHEPWLNEHADFNRVFLIGDSSGGNIVHQV 180

Query: 551 ASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIGSN 730
           A+RAG VDL P+RLAGGIP+HPGFVR  RS+SELE P+SPFLTLDM DKFL+ ALP+GS 
Sbjct: 181 AARAGEVDLGPLRLAGGIPVHPGFVRVERSKSELEQPESPFLTLDMADKFLALALPLGST 240

Query: 731 KDHPITCPMGSAAPPL 778
           K+HPITCPMGSAAPP+
Sbjct: 241 KNHPITCPMGSAAPPI 256


>ref|XP_002525545.1| PREDICTED: probable carboxylesterase 15 [Ricinus communis]
 gb|EEF36804.1| catalytic, putative [Ricinus communis]
          Length = 337

 Score =  389 bits (1000), Expect = e-133
 Identities = 178/258 (68%), Positives = 216/258 (83%), Gaps = 2/258 (0%)
 Frame = +2

Query: 11  MVQEKKLVEEVSGWLRIYDDGSVDRTWTGPPEVNFMIKQVPPHDQFIDGVAIRDVTTTTG 190
           MV+E+KLV+EVSGWLR+YDDGSVDRTWTGPP+V FM + VP HD FIDGVA++D+T  + 
Sbjct: 1   MVRERKLVDEVSGWLRLYDDGSVDRTWTGPPQVKFMSEPVPAHDNFIDGVAVKDLTIDSE 60

Query: 191 SNQRVRLYLP--KKTPKNSNKLPILFHLHGGGFCISEPDWFMYYSIYPQFVQSTQTICVS 364
           S  RVR+YLP  K   +N NKLPI+ H HGGGFCIS+ DW+MYY+IY +  +S   ICVS
Sbjct: 61  SGLRVRIYLPENKNQKQNYNKLPIIIHFHGGGFCISQADWYMYYNIYTRLARSANAICVS 120

Query: 365 PFLRRAPEHRLPAIIDDAFAALQWLQSVARGDANDPWLEEHGDFSRVFLIGDSSGGNLVH 544
            +LR APEHRLPA +DD F+ L WL+S+A+G++ +PW+ ++GDF+RVFLIGDSSG NLVH
Sbjct: 121 VYLRLAPEHRLPAAVDDGFSTLLWLRSLAKGESYEPWVNDYGDFTRVFLIGDSSGANLVH 180

Query: 545 ELASRAGSVDLSPVRLAGGIPIHPGFVRSNRSRSELEMPQSPFLTLDMLDKFLSFALPIG 724
           E++SRAG VDL+PV LAGGIPIHPGFVRS RS+SELE P+SPFLTLDM+DKFL FALP+G
Sbjct: 181 EVSSRAGRVDLTPVILAGGIPIHPGFVRSERSKSELEQPESPFLTLDMVDKFLGFALPVG 240

Query: 725 SNKDHPITCPMGSAAPPL 778
             KDHPITCPMGS APPL
Sbjct: 241 CTKDHPITCPMGSGAPPL 258