BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00005265
         (1308 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arieti...   309   2e-90
gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium prate...   305   7e-89
dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subt...   303   1e-88
sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName:...   301   1e-87
dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subt...   299   6e-87
ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago tru...   295   3e-85
ref|XP_007148908.1| hypothetical protein PHAVU_005G024200g [Phas...   288   4e-83
gb|KRH32967.1| hypothetical protein GLYMA_10G090000 [Glycine max]     284   1e-81
ref|XP_014618555.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisom...   284   1e-81
ref|XP_003545986.1| PREDICTED: DNA topoisomerase 2-like [Glycine...   284   2e-81
ref|XP_014501355.1| DNA topoisomerase 2 [Vigna radiata var. radi...   283   2e-81
ref|XP_023521325.1| DNA topoisomerase 2-like [Cucurbita pepo sub...   271   6e-81
ref|XP_017425524.1| PREDICTED: DNA topoisomerase 2 [Vigna angula...   280   4e-80
dbj|GAU30192.1| hypothetical protein TSUD_311430 [Trifolium subt...   278   9e-80
gb|PHU30238.1| DNA topoisomerase 2 [Capsicum chinense]                258   1e-79
gb|KYP71391.1| DNA topoisomerase 2 [Cajanus cajan]                    278   2e-79
ref|XP_020212372.1| DNA topoisomerase 2 [Cajanus cajan]               278   2e-79
ref|XP_022982563.1| DNA topoisomerase 2 isoform X2 [Cucurbita ma...   273   1e-77
ref|XP_022982562.1| DNA topoisomerase 2 isoform X1 [Cucurbita ma...   273   1e-77
ref|XP_011621441.1| DNA topoisomerase 2 isoform X2 [Amborella tr...   273   1e-77

>ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
 ref|XP_004517303.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
          Length = 1472

 Score =  309 bits (792), Expect = 2e-90
 Identities = 170/274 (62%), Positives = 203/274 (74%), Gaps = 16/274 (5%)
 Frame = -1

Query: 1182 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 1012
            MEK PL+TSNA NI S+ +E   Q+      IL     ++G +++ TQNLW+YEND+MV+
Sbjct: 1    MEKRPLQTSNATNIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYENDEMVH 60

Query: 1011 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEINPE 832
            RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+IDP  NT+SVY NG G+ VEI+ E
Sbjct: 61   RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQE 120

Query: 831  TNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NKYKQ 685
              VY+PEL +      SN++  +K   G R G+GAKLTNI+STEF+IETADGR   KYKQ
Sbjct: 121  EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFVIETADGRRLKKYKQ 180

Query: 684  VFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAGFL 505
            VF+NNM +KS P +TKCK  E WTKVTFKPDL+KF M  L EED+V LMKKRVLDMAG L
Sbjct: 181  VFSNNMGNKSEPVITKCKSSENWTKVTFKPDLEKFKMTYL-EEDVVALMKKRVLDMAGCL 239

Query: 504  GKTVKVELNGQLFRI--IKSCDDLNMKSDEKSKP 409
            GKTVKVELNG L RI   +   DL MKS EKSKP
Sbjct: 240  GKTVKVELNGTLLRIKSFRDYADLFMKSAEKSKP 273



 Score =  185 bits (469), Expect = 3e-47
 Identities = 102/142 (71%), Positives = 107/142 (75%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE     IELAF KKKA+DRKTWIR  +  TF  D + KLIN+K F NKE  LFSRADLQ
Sbjct: 630  DELDGNAIELAFSKKKAEDRKTWIRNFEPGTFR-DHDAKLINYKDFVNKELILFSRADLQ 688

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K I VGQFIGYVS HS Y HHG QSLASTIIGM
Sbjct: 689  RSIPSMVDGLKPGQRKILFCSFKKKLFKEIKVGQFIGYVSEHSCY-HHGEQSLASTIIGM 747

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLLKPNGQFGTR
Sbjct: 748  AQDFVGSNNINLLKPNGQFGTR 769


>gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium pratense]
          Length = 1492

 Score =  305 bits (781), Expect = 7e-89
 Identities = 170/275 (61%), Positives = 203/275 (73%), Gaps = 16/275 (5%)
 Frame = -1

Query: 1182 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 1012
            MEK PL+TSNAANI S+ +E   Q+      IL     ++G +++ TQNLWVYEND+MV 
Sbjct: 1    MEKRPLQTSNAANIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVN 60

Query: 1011 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEINPE 832
            RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+IDP  NT+SVY NG G+ VEI+ E
Sbjct: 61   RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQE 120

Query: 831  TNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NKYKQ 685
              VY+PEL +      SN++  +K   G R G+GAKLTNI+STEFIIETADGR   KYKQ
Sbjct: 121  EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETADGRRLKKYKQ 180

Query: 684  VFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAGFL 505
            VF++NM  KS P +TKCK  E WTKVTFKPDL+KF M  L EED+V LMKKRVLDMAG L
Sbjct: 181  VFSDNMGKKSEPVITKCKASENWTKVTFKPDLEKFKMTYL-EEDVVALMKKRVLDMAGCL 239

Query: 504  GKTVKVELNGQL--FRIIKSCDDLNMKSDEKSKPV 406
            GKTVKVELNG L  F+  +   DL +KS EKSKP+
Sbjct: 240  GKTVKVELNGTLIRFKSFRDYADLFLKSAEKSKPM 274



 Score =  182 bits (462), Expect = 2e-46
 Identities = 102/142 (71%), Positives = 106/142 (74%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE     IELAF KKKA+DRKTWIR  +  T   D E KLIN+K F NKE  LFS ADLQ
Sbjct: 629  DELDGNAIELAFSKKKAEDRKTWIRNFEPGTCR-DHEAKLINYKDFVNKELILFSMADLQ 687

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K I VGQFIGYVS HSAY HHG QSLASTIIGM
Sbjct: 688  RSIPSMVDGLKPGQRKILFCSFKKKLVKEIKVGQFIGYVSEHSAY-HHGEQSLASTIIGM 746

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLLKPNGQFGTR
Sbjct: 747  AQDFVGSNNINLLKPNGQFGTR 768


>dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subterraneum]
          Length = 1336

 Score =  303 bits (777), Expect = 1e-88
 Identities = 169/274 (61%), Positives = 201/274 (73%), Gaps = 16/274 (5%)
 Frame = -1

Query: 1182 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 1012
            MEK PL+TSNA NI S+ +E   Q+      IL     ++G +++ TQNLWVYEND+MV 
Sbjct: 1    MEKRPLQTSNATNIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVQ 60

Query: 1011 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEINPE 832
            RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+IDP  NTISVY NG G+ VEI+ E
Sbjct: 61   RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPERNTISVYNNGDGVPVEIHQE 120

Query: 831  TNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NKYKQ 685
              VY+PEL +      SN++  +K   G R G+GAKLTNI+STEF+IETADGR   KYKQ
Sbjct: 121  EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFVIETADGRRLKKYKQ 180

Query: 684  VFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAGFL 505
            VF+NNM  KS P +TKCK +E WTKVTFKPDL+KF M  L EED+V LMKKRVLDMAG L
Sbjct: 181  VFSNNMGTKSEPVITKCKANENWTKVTFKPDLEKFKMTYL-EEDVVALMKKRVLDMAGCL 239

Query: 504  GKTVKVELNGQL--FRIIKSCDDLNMKSDEKSKP 409
            GKTVKVELNG L  F+  +   DL +K  EKSKP
Sbjct: 240  GKTVKVELNGTLIRFKSFRDYADLFLKCAEKSKP 273


>sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase
            II; AltName: Full=PsTopII
 emb|CAA74891.1| topoisomerase II [Pisum sativum]
          Length = 1462

 Score =  301 bits (771), Expect = 1e-87
 Identities = 166/275 (60%), Positives = 201/275 (73%), Gaps = 16/275 (5%)
 Frame = -1

Query: 1182 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 1012
            MEK PL+TSNAANI S+ +E   Q+      IL     ++G +++ TQNLWVYEND+MV+
Sbjct: 1    MEKRPLQTSNAANIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVH 60

Query: 1011 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEINPE 832
            R++SYVPGLYKIFDEIL++AA+NK  DP MD LKV+IDP  NT+SVY NG G+ VEI+ E
Sbjct: 61   RAVSYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQE 120

Query: 831  TNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NKYKQ 685
              VY+PEL +      SN++  +K   G R G+GAKLTNI+STEFIIETADGR   KYKQ
Sbjct: 121  EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETADGRRLKKYKQ 180

Query: 684  VFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAGFL 505
            VF+NNM  K  P +TKCK  E WTKVTFKPDL+KF M  L EED+V LMKKRVLDMAG  
Sbjct: 181  VFSNNMGTKCEPVITKCKASENWTKVTFKPDLEKFKMAYL-EEDVVALMKKRVLDMAGCF 239

Query: 504  GKTVKVELNGQL--FRIIKSCDDLNMKSDEKSKPV 406
            GKTVKVELNG L  F+  +   DL +K  EKSKP+
Sbjct: 240  GKTVKVELNGTLIRFKSFRDYADLFLKCAEKSKPM 274



 Score =  135 bits (340), Expect = 2e-30
 Identities = 83/141 (58%), Positives = 90/141 (63%)
 Frame = -1

Query: 426 DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
           DE     IELAF KKKA+ RK W+R  +  T   D E KLIN+K F NKE  LFSRADL 
Sbjct: 629 DELDGNAIELAFSKKKAEGRKIWMRNFEPGTCR-DHEAKLINYKDFVNKELILFSRADLF 687

Query: 246 RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            S           ++K+            I V QFIGYVS HSAYHH G QSLASTIIGM
Sbjct: 688 GSF----------KKKLYKE---------IKVAQFIGYVSEHSAYHH-GEQSLASTIIGM 727

Query: 66  AQDFVGSNNINLLKPNGQFGT 4
           AQDFVGSNNINLLKPNGQFGT
Sbjct: 728 AQDFVGSNNINLLKPNGQFGT 748


>dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subterraneum]
          Length = 1359

 Score =  299 bits (765), Expect = 6e-87
 Identities = 167/273 (61%), Positives = 199/273 (72%), Gaps = 16/273 (5%)
 Frame = -1

Query: 1182 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 1012
            MEK PL+TSNA NI S+ +E   Q+      IL     ++G +++ TQNLWVYEND+MV 
Sbjct: 1    MEKRPLQTSNATNIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENDEMVN 60

Query: 1011 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEINPE 832
            RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+IDP  NT+SVY NG G+ VEI+ E
Sbjct: 61   RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQE 120

Query: 831  TNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NKYKQ 685
              VY+PEL +      SN++  +K   G R G+GAKLTNI+STEFIIETADGR   KYKQ
Sbjct: 121  EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFIIETADGRRLKKYKQ 180

Query: 684  VFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAGFL 505
            VF+NNM  K+ P +TKCK  E WTKVTFKPDL+KF M  L EED+V LMKKRVLDMAG L
Sbjct: 181  VFSNNMGKKAEPVITKCKASENWTKVTFKPDLEKFKMTYL-EEDVVALMKKRVLDMAGCL 239

Query: 504  GKTVKVELNGQL--FRIIKSCDDLNMKSDEKSK 412
            GKTVKVELNG L  F+  +   DL +KS EK K
Sbjct: 240  GKTVKVELNGTLIRFKSFRDYADLFLKSAEKYK 272


>ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago truncatula]
 gb|AES69522.2| DNA topoisomerase IV subunit A [Medicago truncatula]
          Length = 1504

 Score =  295 bits (754), Expect = 3e-85
 Identities = 164/274 (59%), Positives = 199/274 (72%), Gaps = 16/274 (5%)
 Frame = -1

Query: 1182 MEKPPLETSNAANILSEKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKMVY 1012
            MEK PL+ S  ANI S+ +E   Q+      IL     ++G +++ TQNLWVYEN++MV 
Sbjct: 1    MEKRPLQPSTTANIPSKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWVYENEEMVN 60

Query: 1011 RSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEINPE 832
            RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV+IDP  NT+SVY NG G+ VEI+ E
Sbjct: 61   RSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVTIDPEANTVSVYNNGDGVPVEIHQE 120

Query: 831  TNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NKYKQ 685
              VY+PEL +      SN++  +K   G R G+GAKLTNI+STEF+IETADGR   KYKQ
Sbjct: 121  EKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFVIETADGRRLKKYKQ 180

Query: 684  VFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAGFL 505
            VFT+NM  KS P +TKCK +E WTKVTFKPDL+KF M  L EED+V LMKKRVLDMAG L
Sbjct: 181  VFTDNMGKKSDPVITKCKANENWTKVTFKPDLEKFKMTYL-EEDVVALMKKRVLDMAGCL 239

Query: 504  GKTVKVELNGQL--FRIIKSCDDLNMKSDEKSKP 409
            GKTVKVELNG +  F+  K   +L +K  EKSKP
Sbjct: 240  GKTVKVELNGTVIRFKSFKDYAELFLKCAEKSKP 273



 Score =  173 bits (438), Expect = 3e-43
 Identities = 95/135 (70%), Positives = 103/135 (76%)
 Frame = -1

Query: 405  IELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQRSIPSLV 226
            IELAF KKKA+DRK W+R  +  T   D E KLI++K F +KE  LFSRADLQRSIPS+V
Sbjct: 637  IELAFSKKKAEDRKNWMRSFEPGTCR-DHEAKLISYKDFVDKELILFSRADLQRSIPSMV 695

Query: 225  DGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGMAQDFVGS 46
            DGLK GQRKI          K + VGQFIGYVS HSAY HHG QSLASTIIGMAQDFVGS
Sbjct: 696  DGLKPGQRKILFCSFKKNLSKEVKVGQFIGYVSEHSAY-HHGEQSLASTIIGMAQDFVGS 754

Query: 45   NNINLLKPNGQFGTR 1
            NNINLL PNGQFGTR
Sbjct: 755  NNINLLTPNGQFGTR 769


>ref|XP_007148908.1| hypothetical protein PHAVU_005G024200g [Phaseolus vulgaris]
 gb|ESW20902.1| hypothetical protein PHAVU_005G024200g [Phaseolus vulgaris]
          Length = 1474

 Score =  288 bits (738), Expect = 4e-83
 Identities = 160/276 (57%), Positives = 198/276 (71%), Gaps = 19/276 (6%)
 Frame = -1

Query: 1176 KPPLETSNAANILS------EKLEASNQRHLDILCFRTMFLGPMDERTQNLWVYENDKMV 1015
            K PL++S+AAN+ +      E++     +   IL     ++G +++ TQ LWVYEND+MV
Sbjct: 6    KRPLQSSSAANVAAAAGKTIEEVYQKKTQLEHILLRPDTYVGSIEKHTQTLWVYENDEMV 65

Query: 1014 YRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEINP 835
             R ISYVPGLYKIFDEIL++AA+NK  DP MD LKV+ID   NT+SV+ NG G+ +EI+ 
Sbjct: 66   QRPISYVPGLYKIFDEILVNAADNKQRDPSMDALKVTIDAEQNTVSVFNNGDGVPIEIHQ 125

Query: 834  ETNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NKYK 688
            E  VY+PEL +      SN++  +K   G R G+GAKLTNI+STEF+IETADG+   KYK
Sbjct: 126  EEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFVIETADGKRLKKYK 185

Query: 687  QVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAGF 508
            QVF+NNM  KS P +TKCK  E WTKVTFKPDL KF MD L EED+V LMKKRVLDMAG 
Sbjct: 186  QVFSNNMGKKSEPVITKCKAGENWTKVTFKPDLAKFRMDHL-EEDVVALMKKRVLDMAGC 244

Query: 507  LGKTVKVELNGQLFRI--IKSCDDLNMKSDEKSKPV 406
            LGKTVKVELNGQL R+   +   DL +KS EKSKPV
Sbjct: 245  LGKTVKVELNGQLIRMKSFRDYADLYLKSAEKSKPV 280



 Score =  176 bits (445), Expect = 4e-44
 Identities = 95/142 (66%), Positives = 105/142 (73%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE+    IE+AF KKKA+DRKTWIR  +  TF  D   + IN++ F NKE  LFSRADLQ
Sbjct: 636  DEQDGSAIEMAFSKKKAEDRKTWIRNFEPGTFR-DHTARYINYRDFVNKELILFSRADLQ 694

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K I V QFIGYVS HSAY HHG QSL+STII M
Sbjct: 695  RSIPSMVDGLKPGQRKILFCSFKKKLFKEIKVAQFIGYVSEHSAY-HHGEQSLSSTIINM 753

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNN+NLLKPNGQFGTR
Sbjct: 754  AQDFVGSNNVNLLKPNGQFGTR 775


>gb|KRH32967.1| hypothetical protein GLYMA_10G090000 [Glycine max]
          Length = 1459

 Score =  284 bits (727), Expect = 1e-81
 Identities = 157/278 (56%), Positives = 199/278 (71%), Gaps = 21/278 (7%)
 Frame = -1

Query: 1176 KPPLETSNAANILS--------EKLEASNQRHLDILCFRTMFLGPMDERTQNLWVYENDK 1021
            K PL++S+AAN+ +        E++     +   IL     ++G +++ TQ LWVYEND+
Sbjct: 6    KYPLQSSSAANVAATGGGKKTIEEMYQKKTQLEHILLRPDTYVGSVEKHTQTLWVYENDE 65

Query: 1020 MVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEI 841
            MV R++SYVPGLYKIFDEIL++AA+NK  DP MD LKVSID   NT+S++ NG G+ VEI
Sbjct: 66   MVQRAVSYVPGLYKIFDEILVNAADNKQRDPSMDALKVSIDAEANTVSIFNNGDGVPVEI 125

Query: 840  NPETNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NK 694
            + E  VY+PEL +      SN+N   K   G R G+GAKLTNI+STEF+IETADG+   K
Sbjct: 126  HQEEKVYVPELIFGHLLTSSNYNDNEKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQKK 185

Query: 693  YKQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMA 514
            YKQVF++NM  KS P +TKCK  E WTKVTFKPDL+KF MD L EED+V LMKKRV+DMA
Sbjct: 186  YKQVFSDNMGKKSEPVITKCKVGENWTKVTFKPDLEKFKMDYL-EEDVVALMKKRVVDMA 244

Query: 513  GFLGKTVKVELNGQLFRI--IKSCDDLNMKSDEKSKPV 406
            G LGKTVKVELNGQ+ R+   +   DL +KS EKS+PV
Sbjct: 245  GCLGKTVKVELNGQVIRMKSFRDYADLYLKSVEKSRPV 282



 Score =  173 bits (439), Expect = 3e-43
 Identities = 96/142 (67%), Positives = 104/142 (73%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE+    IE+AF KKKA+DRK WIR  +  T   D   K IN++ F NKE  L+SRADLQ
Sbjct: 638  DEQDGGAIEMAFSKKKAEDRKIWIRNFEPGTCR-DHMAKYINYRDFVNKELILYSRADLQ 696

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K I VGQFIGYVS HSAY HHG QSLASTII M
Sbjct: 697  RSIPSMVDGLKPGQRKILFCSFKKKLFKEIKVGQFIGYVSEHSAY-HHGEQSLASTIINM 755

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLLKPNGQFGTR
Sbjct: 756  AQDFVGSNNINLLKPNGQFGTR 777


>ref|XP_014618555.1| PREDICTED: LOW QUALITY PROTEIN: DNA topoisomerase 2-like [Glycine
            max]
          Length = 1481

 Score =  284 bits (727), Expect = 1e-81
 Identities = 157/278 (56%), Positives = 199/278 (71%), Gaps = 21/278 (7%)
 Frame = -1

Query: 1176 KPPLETSNAANILS--------EKLEASNQRHLDILCFRTMFLGPMDERTQNLWVYENDK 1021
            K PL++S+AAN+ +        E++     +   IL     ++G +++ TQ LWVYEND+
Sbjct: 6    KYPLQSSSAANVAATGGGKKTIEEMYQKKTQLEHILLRPDTYVGSVEKHTQTLWVYENDE 65

Query: 1020 MVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEI 841
            MV R++SYVPGLYKIFDEIL++AA+NK  DP MD LKVSID   NT+S++ NG G+ VEI
Sbjct: 66   MVQRAVSYVPGLYKIFDEILVNAADNKQRDPSMDALKVSIDAEANTVSIFNNGDGVPVEI 125

Query: 840  NPETNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NK 694
            + E  VY+PEL +      SN+N   K   G R G+GAKLTNI+STEF+IETADG+   K
Sbjct: 126  HQEEKVYVPELIFGHLLTSSNYNDNEKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQKK 185

Query: 693  YKQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMA 514
            YKQVF++NM  KS P +TKCK  E WTKVTFKPDL+KF MD L EED+V LMKKRV+DMA
Sbjct: 186  YKQVFSDNMGKKSEPVITKCKVGENWTKVTFKPDLEKFKMDYL-EEDVVALMKKRVVDMA 244

Query: 513  GFLGKTVKVELNGQLFRI--IKSCDDLNMKSDEKSKPV 406
            G LGKTVKVELNGQ+ R+   +   DL +KS EKS+PV
Sbjct: 245  GCLGKTVKVELNGQVIRMKSFRDYADLYLKSVEKSRPV 282



 Score =  173 bits (439), Expect = 3e-43
 Identities = 96/142 (67%), Positives = 104/142 (73%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE+    IE+AF KKKA+DRK WIR  +  T   D   K IN++ F NKE  L+SRADLQ
Sbjct: 638  DEQDGGAIEMAFSKKKAEDRKIWIRNFEPGTCR-DHMAKYINYRDFVNKELILYSRADLQ 696

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K I VGQFIGYVS HSAY HHG QSLASTII M
Sbjct: 697  RSIPSMVDGLKPGQRKILFCSFKKKLFKEIKVGQFIGYVSEHSAY-HHGEQSLASTIINM 755

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLLKPNGQFGTR
Sbjct: 756  AQDFVGSNNINLLKPNGQFGTR 777


>ref|XP_003545986.1| PREDICTED: DNA topoisomerase 2-like [Glycine max]
 gb|KRH13973.1| hypothetical protein GLYMA_15G276000 [Glycine max]
          Length = 1484

 Score =  284 bits (726), Expect = 2e-81
 Identities = 156/277 (56%), Positives = 199/277 (71%), Gaps = 22/277 (7%)
 Frame = -1

Query: 1170 PLETSNAANILS---------EKLEASNQRHLDILCFRTMFLGPMDERTQNLWVYENDKM 1018
            PL++S+AAN+ +         E++     +   IL     ++G +++ TQ LWVYEND+M
Sbjct: 8    PLQSSSAANVAAAAATGKKTIEEMYQKKTQLEHILLRPDTYVGSVEKHTQTLWVYENDEM 67

Query: 1017 VYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEIN 838
            V+R++SYVPGLYKIFDEIL++AA+NK  DP MD LKV+I+   NT+SV+ NG G+ VEI+
Sbjct: 68   VHRAVSYVPGLYKIFDEILVNAADNKQRDPSMDALKVTINAEANTVSVFNNGDGVPVEIH 127

Query: 837  PETNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NKY 691
             E  VY+PEL +      SN+N   K   G R G+GAKLTNI+STEF+IETADG+   KY
Sbjct: 128  QEEKVYVPELIFGHLLTSSNYNDNEKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQKKY 187

Query: 690  KQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAG 511
            KQVF+NNM  KS P +TKCK  E WTKVTFKPDL+KF MD L EED+V LMKKRV+DMAG
Sbjct: 188  KQVFSNNMGKKSEPVITKCKVGENWTKVTFKPDLEKFKMDYL-EEDVVALMKKRVVDMAG 246

Query: 510  FLGKTVKVELNGQLFRI--IKSCDDLNMKSDEKSKPV 406
             LGKTVKVELNGQ+ R+   +   DL +KS EKS+PV
Sbjct: 247  CLGKTVKVELNGQVIRMKSFRDYADLYLKSAEKSRPV 283



 Score =  173 bits (439), Expect = 3e-43
 Identities = 96/142 (67%), Positives = 104/142 (73%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE+    IE+AF KKKA+DRK WIR  +  T   D   K IN++ F NKE  L+SRADLQ
Sbjct: 639  DEQDGGAIEMAFSKKKAEDRKIWIRNFEPGTCR-DHMAKYINYRDFVNKELILYSRADLQ 697

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K I VGQFIGYVS HSAY HHG QSLASTII M
Sbjct: 698  RSIPSMVDGLKPGQRKILFCSFKKKLSKEIKVGQFIGYVSEHSAY-HHGEQSLASTIINM 756

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLLKPNGQFGTR
Sbjct: 757  AQDFVGSNNINLLKPNGQFGTR 778


>ref|XP_014501355.1| DNA topoisomerase 2 [Vigna radiata var. radiata]
          Length = 1492

 Score =  283 bits (725), Expect = 2e-81
 Identities = 157/276 (56%), Positives = 197/276 (71%), Gaps = 19/276 (6%)
 Frame = -1

Query: 1176 KPPLETSNAANILS------EKLEASNQRHLDILCFRTMFLGPMDERTQNLWVYENDKMV 1015
            K PL++S+AAN+ +      E++     +   IL     ++G +++ TQ LWVY+N +MV
Sbjct: 6    KRPLQSSSAANVAAAAGKTIEEVYQKKTQLEHILLRPDTYVGSIEKHTQTLWVYQNGEMV 65

Query: 1014 YRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEINP 835
             R +SYVPGLYKIFDEIL++AA+NK  DP MD LKV+ID   NT+SVY NG G+ VEI+ 
Sbjct: 66   QRPVSYVPGLYKIFDEILVNAADNKQRDPSMDALKVTIDAEQNTVSVYNNGDGVPVEIHQ 125

Query: 834  ETNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NKYK 688
            E  VY+PEL +      SN++  +K   G R G+GAKLTNI+STEF+IETADG+   KYK
Sbjct: 126  EEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQKKYK 185

Query: 687  QVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAGF 508
            QVF+NNM  KS P +TKCK  E WTKVTFKPDL KF MD L EED+V LMKKRV+DMAG 
Sbjct: 186  QVFSNNMGKKSEPVITKCKGGENWTKVTFKPDLAKFKMDHL-EEDVVALMKKRVVDMAGC 244

Query: 507  LGKTVKVELNGQLFRI--IKSCDDLNMKSDEKSKPV 406
            LGKTVKVELNGQ+ R+   +   DL +KS EKSKPV
Sbjct: 245  LGKTVKVELNGQVIRMKSFRDYADLYLKSAEKSKPV 280



 Score =  175 bits (444), Expect = 6e-44
 Identities = 95/142 (66%), Positives = 105/142 (73%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE+    IE+AF KKKA+DRKTWIR  +  T+  D   + IN++ F NKE  LFSRADLQ
Sbjct: 635  DEQDGSAIEMAFSKKKAEDRKTWIRNFEPGTYR-DHSARYINYRDFVNKELILFSRADLQ 693

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K I V QFIGYVS HSAY HHG QSL+STII M
Sbjct: 694  RSIPSMVDGLKPGQRKILFCSFKKKLFKEIKVAQFIGYVSEHSAY-HHGEQSLSSTIINM 752

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLLKPNGQFGTR
Sbjct: 753  AQDFVGSNNINLLKPNGQFGTR 774


>ref|XP_023521325.1| DNA topoisomerase 2-like [Cucurbita pepo subsp. pepo]
          Length = 615

 Score =  271 bits (692), Expect = 6e-81
 Identities = 147/256 (57%), Positives = 181/256 (70%), Gaps = 20/256 (7%)
 Frame = -1

Query: 1179 EKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYEN 1027
            +K PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLW+YEN
Sbjct: 5    KKRPLQNSNAANVDSDPGGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYEN 64

Query: 1026 DKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISV 847
            DKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV ID   N ISV+ NG G+ V
Sbjct: 65   DKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNFISVFNNGDGVPV 124

Query: 846  EINPETNVYLPEL---------NYSNFNYGLKGTRTGHGAKLTNIYSTEFIIETADGR-- 700
            E++ E  VY+PEL         NY +      G R G+GAKLTNI+STEF+IETADGR  
Sbjct: 125  EVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETADGRRL 184

Query: 699  NKYKQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLD 520
             KYKQVF++NM  KS P +TKCKE + WTKVTFKPDL KF M  L E+D+V LMKKRVLD
Sbjct: 185  KKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHL-EDDVVALMKKRVLD 243

Query: 519  MAGFLGKTVKVELNGQ 472
            +AG LGKTVKVELNGQ
Sbjct: 244  LAGCLGKTVKVELNGQ 259


>ref|XP_017425524.1| PREDICTED: DNA topoisomerase 2 [Vigna angularis]
 gb|KOM42453.1| hypothetical protein LR48_Vigan05g005700 [Vigna angularis]
 dbj|BAT93356.1| hypothetical protein VIGAN_07230300 [Vigna angularis var. angularis]
          Length = 1480

 Score =  280 bits (716), Expect = 4e-80
 Identities = 156/276 (56%), Positives = 196/276 (71%), Gaps = 19/276 (6%)
 Frame = -1

Query: 1176 KPPLETSNAANILS------EKLEASNQRHLDILCFRTMFLGPMDERTQNLWVYENDKMV 1015
            K PL++S+AAN+ +      E++     +   IL     ++G +++ TQ LWVYE+  MV
Sbjct: 6    KRPLQSSSAANVAAAAGKTIEEVYQKKTQLEHILLRPDTYVGSIEKHTQTLWVYEDGGMV 65

Query: 1014 YRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEINP 835
             R +SYVPGLYKIFDEIL++AA+NK  DP MD LKV+ID   NT+SV+ NG G+ VEI+ 
Sbjct: 66   QRPVSYVPGLYKIFDEILVNAADNKQRDPSMDALKVTIDAEQNTVSVFNNGDGVPVEIHQ 125

Query: 834  ETNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NKYK 688
            E  VY+PEL +      SN++  +K   G R G+GAKLTNI+STEF+IETADG+   KYK
Sbjct: 126  EEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQKKYK 185

Query: 687  QVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAGF 508
            QVF+NNM  KS P +TKCK  E WTKVTFKPDL KF MD L EED+V LMKKRV+DMAG 
Sbjct: 186  QVFSNNMGKKSEPVITKCKGGENWTKVTFKPDLAKFKMDHL-EEDVVALMKKRVVDMAGC 244

Query: 507  LGKTVKVELNGQLFRI--IKSCDDLNMKSDEKSKPV 406
            LGKTVKVELNGQL R+   +   DL +KS EKSKP+
Sbjct: 245  LGKTVKVELNGQLIRMKSFRDYADLYLKSAEKSKPM 280



 Score =  176 bits (447), Expect = 2e-44
 Identities = 96/142 (67%), Positives = 105/142 (73%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE+    IE+AF KKKA+DRKTWIR  +  TF  D   + IN++ F NKE  LFSRADLQ
Sbjct: 635  DEQDGSAIEMAFSKKKAEDRKTWIRNFEPGTFR-DHSARYINYRDFVNKELILFSRADLQ 693

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K I V QFIGYVS HSAY HHG QSL+STII M
Sbjct: 694  RSIPSMVDGLKPGQRKILFCSFKKKLFKEIKVAQFIGYVSEHSAY-HHGEQSLSSTIINM 752

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLLKPNGQFGTR
Sbjct: 753  AQDFVGSNNINLLKPNGQFGTR 774


>dbj|GAU30192.1| hypothetical protein TSUD_311430 [Trifolium subterraneum]
          Length = 1252

 Score =  278 bits (711), Expect = 9e-80
 Identities = 149/236 (63%), Positives = 180/236 (76%), Gaps = 13/236 (5%)
 Frame = -1

Query: 1077 FLGPMDERTQNLWVYENDKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSID 898
            ++G +++ TQNLWVYEN++MV+RSISYVPGLYKIFDEIL++AA+NK  D  MD LKV+ID
Sbjct: 25   YVGSIEKHTQNLWVYENEEMVHRSISYVPGLYKIFDEILVNAADNKQRDSSMDSLKVTID 84

Query: 897  PSTNTISVYYNGSGISVEINPETNVYLPELNY------SNFNYGLK---GTRTGHGAKLT 745
            P  NTIS+Y NG GI VEI+ E  VY+PEL +      SN++  +K   G R G+GAKLT
Sbjct: 85   PELNTISIYNNGDGIPVEIHQEEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLT 144

Query: 744  NIYSTEFIIETADGR--NKYKQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMD 571
            NI+STEF+IETADGR   KYKQVF+NNM  KS P +TKCK ++ WTKVTFKPDL+KF M 
Sbjct: 145  NIFSTEFVIETADGRRLKKYKQVFSNNMGKKSKPVITKCKANQNWTKVTFKPDLEKFKMT 204

Query: 570  SLKEEDIVGLMKKRVLDMAGFLGKTVKVELNGQL--FRIIKSCDDLNMKSDEKSKP 409
             L EED+V LMKKRVLDMAG  GKTVKVELNG L  F+  +   DL +K  EKSKP
Sbjct: 205  YL-EEDVVALMKKRVLDMAGCFGKTVKVELNGTLIRFKSFRDYADLFLKCAEKSKP 259


>gb|PHU30238.1| DNA topoisomerase 2 [Capsicum chinense]
          Length = 318

 Score =  258 bits (659), Expect = 1e-79
 Identities = 141/253 (55%), Positives = 177/253 (69%), Gaps = 17/253 (6%)
 Frame = -1

Query: 1179 EKPPLETSNAANILS---EKLEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYENDKM 1018
            +K PL++SN ANI     + +E + Q+      IL     ++G +++ TQ LWV+END M
Sbjct: 4    KKLPLQSSNNANISKANGKTIEQTYQKKTQLEHILLRPETYIGSVEKHTQTLWVWENDAM 63

Query: 1017 VYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEIN 838
            V+R ++YVPGLYKIFDEIL++AA+NK  DPKMD L+V IDP  N ISVY NG GI VEI+
Sbjct: 64   VHRPVTYVPGLYKIFDEILVNAADNKQRDPKMDALEVVIDPEQNFISVYNNGDGIPVEIH 123

Query: 837  PETNVYLPEL---------NYSNFNYGLKGTRTGHGAKLTNIYSTEFIIETADGR--NKY 691
             E  VY+PEL         NY +      G R G+GAKLTNI+STEF+IETADG+   KY
Sbjct: 124  SEEGVYVPELIFGHLLTSSNYDDDEKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQRKY 183

Query: 690  KQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMAG 511
            KQVF+NNM  KS P +TKCK  E WTKV+FKPDL KF M+ L E D+V LM+KRV+D+ G
Sbjct: 184  KQVFSNNMGKKSEPIITKCKASENWTKVSFKPDLAKFNMEQL-EADVVALMRKRVIDLGG 242

Query: 510  FLGKTVKVELNGQ 472
             LGKTVKV+LN Q
Sbjct: 243  CLGKTVKVKLNEQ 255


>gb|KYP71391.1| DNA topoisomerase 2 [Cajanus cajan]
          Length = 1446

 Score =  278 bits (711), Expect = 2e-79
 Identities = 154/278 (55%), Positives = 199/278 (71%), Gaps = 19/278 (6%)
 Frame = -1

Query: 1182 MEKP-PLETSNAANILS-----EKLEASNQRHLDILCFRTMFLGPMDERTQNLWVYENDK 1021
            M+ P PL +S+AAN+ +     E++     +   IL     ++G +++ TQ LWVYEND+
Sbjct: 1    MDHPRPLHSSSAANVAAAGKTIEEMYQKKTQLEHILLRPDTYVGSVEKHTQTLWVYENDE 60

Query: 1020 MVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEI 841
            MV+R +SYVPGLYKIFDEIL++AA+NK  DP MD LKV +D   NTI+V+ NG G+ VEI
Sbjct: 61   MVHRPVSYVPGLYKIFDEILVNAADNKQRDPSMDALKVLVDAEQNTITVFNNGDGVPVEI 120

Query: 840  NPETNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NK 694
            + E  VY+PEL +      SN++  +K   G R G+GAKLTNI+STEF+IETADG+   K
Sbjct: 121  HQEEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQKK 180

Query: 693  YKQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMA 514
            YKQVF+NNM  KS P +TKCK  E WTKVTFKPDL+KF M  L EED+V LMKKRV+DMA
Sbjct: 181  YKQVFSNNMGKKSEPVITKCKSGENWTKVTFKPDLEKFKMAYL-EEDVVALMKKRVVDMA 239

Query: 513  GFLGKTVKVELNGQLFRI--IKSCDDLNMKSDEKSKPV 406
            G LGKTVKVELNG++ ++   +   DL +KS EKS+PV
Sbjct: 240  GCLGKTVKVELNGRVIKMKSFRDYADLYLKSAEKSRPV 277



 Score =  173 bits (439), Expect = 3e-43
 Identities = 95/142 (66%), Positives = 103/142 (72%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE+    IE+AF KKK +DRK WIR  +      D  D+ IN++ F NKE  LFSRADLQ
Sbjct: 633  DEQDGNAIEMAFSKKKTEDRKIWIRNFEPGNHR-DHMDRYINYRDFVNKELILFSRADLQ 691

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K I VGQFIGYVS HSAY HHG QSLASTII M
Sbjct: 692  RSIPSMVDGLKPGQRKILFCSFKKKLFKEIKVGQFIGYVSEHSAY-HHGEQSLASTIINM 750

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLLKPNGQFGTR
Sbjct: 751  AQDFVGSNNINLLKPNGQFGTR 772


>ref|XP_020212372.1| DNA topoisomerase 2 [Cajanus cajan]
          Length = 1480

 Score =  278 bits (711), Expect = 2e-79
 Identities = 154/278 (55%), Positives = 199/278 (71%), Gaps = 19/278 (6%)
 Frame = -1

Query: 1182 MEKP-PLETSNAANILS-----EKLEASNQRHLDILCFRTMFLGPMDERTQNLWVYENDK 1021
            M+ P PL +S+AAN+ +     E++     +   IL     ++G +++ TQ LWVYEND+
Sbjct: 1    MDHPRPLHSSSAANVAAAGKTIEEMYQKKTQLEHILLRPDTYVGSVEKHTQTLWVYENDE 60

Query: 1020 MVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGISVEI 841
            MV+R +SYVPGLYKIFDEIL++AA+NK  DP MD LKV +D   NTI+V+ NG G+ VEI
Sbjct: 61   MVHRPVSYVPGLYKIFDEILVNAADNKQRDPSMDALKVLVDAEQNTITVFNNGDGVPVEI 120

Query: 840  NPETNVYLPELNY------SNFNYGLK---GTRTGHGAKLTNIYSTEFIIETADGR--NK 694
            + E  VY+PEL +      SN++  +K   G R G+GAKLTNI+STEF+IETADG+   K
Sbjct: 121  HQEEKVYVPELIFGHLLTSSNYDDNVKKTTGGRNGYGAKLTNIFSTEFVIETADGKRQKK 180

Query: 693  YKQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVLDMA 514
            YKQVF+NNM  KS P +TKCK  E WTKVTFKPDL+KF M  L EED+V LMKKRV+DMA
Sbjct: 181  YKQVFSNNMGKKSEPVITKCKSGENWTKVTFKPDLEKFKMAYL-EEDVVALMKKRVVDMA 239

Query: 513  GFLGKTVKVELNGQLFRI--IKSCDDLNMKSDEKSKPV 406
            G LGKTVKVELNG++ ++   +   DL +KS EKS+PV
Sbjct: 240  GCLGKTVKVELNGRVIKMKSFRDYADLYLKSAEKSRPV 277



 Score =  173 bits (439), Expect = 3e-43
 Identities = 95/142 (66%), Positives = 103/142 (72%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE+    IE+AF KKK +DRK WIR  +      D  D+ IN++ F NKE  LFSRADLQ
Sbjct: 633  DEQDGNAIEMAFSKKKTEDRKIWIRNFEPGNHR-DHMDRYINYRDFVNKELILFSRADLQ 691

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K I VGQFIGYVS HSAY HHG QSLASTII M
Sbjct: 692  RSIPSMVDGLKPGQRKILFCSFKKKLFKEIKVGQFIGYVSEHSAY-HHGEQSLASTIINM 750

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLLKPNGQFGTR
Sbjct: 751  AQDFVGSNNINLLKPNGQFGTR 772


>ref|XP_022982563.1| DNA topoisomerase 2 isoform X2 [Cucurbita maxima]
          Length = 1490

 Score =  273 bits (698), Expect = 1e-77
 Identities = 148/257 (57%), Positives = 182/257 (70%), Gaps = 20/257 (7%)
 Frame = -1

Query: 1182 MEKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYE 1030
            +EK PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLW+YE
Sbjct: 3    VEKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYE 62

Query: 1029 NDKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGIS 850
            NDKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV ID   N ISV+ NG G+ 
Sbjct: 63   NDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNCISVFNNGDGVP 122

Query: 849  VEINPETNVYLPEL---------NYSNFNYGLKGTRTGHGAKLTNIYSTEFIIETADGR- 700
            VE++ E  VY+PEL         NY +      G R G+GAKLTNI+STEF+IETADGR 
Sbjct: 123  VEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETADGRR 182

Query: 699  -NKYKQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVL 523
              KYKQVF++NM  KS P +TKCKE + WTKVTFKPDL KF M  L E+D+V LMKKRVL
Sbjct: 183  LKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHL-EDDVVALMKKRVL 241

Query: 522  DMAGFLGKTVKVELNGQ 472
            D+AG LGKTVKVELNGQ
Sbjct: 242  DLAGCLGKTVKVELNGQ 258



 Score =  166 bits (419), Expect = 1e-40
 Identities = 91/142 (64%), Positives = 101/142 (71%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE     IELAF KKK ++RK W+R+ +  T  +D+ +KLI +  F NKE  LFS ADLQ
Sbjct: 639  DEHDGEAIELAFSKKKIEERKNWLRQFEAGTH-LDQSEKLIKYSDFVNKELILFSMADLQ 697

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K   V QF GYVS HSAY HHG QSLASTIIGM
Sbjct: 698  RSIPSMVDGLKPGQRKILFCSFKRNFIKEAKVSQFSGYVSEHSAY-HHGEQSLASTIIGM 756

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLL PNGQFGTR
Sbjct: 757  AQDFVGSNNINLLLPNGQFGTR 778


>ref|XP_022982562.1| DNA topoisomerase 2 isoform X1 [Cucurbita maxima]
          Length = 1491

 Score =  273 bits (698), Expect = 1e-77
 Identities = 148/257 (57%), Positives = 182/257 (70%), Gaps = 20/257 (7%)
 Frame = -1

Query: 1182 MEKPPLETSNAANILSEK------LEASNQRHLD---ILCFRTMFLGPMDERTQNLWVYE 1030
            +EK PL+ SNAAN+ S+       +E   Q+      IL     ++G +++ TQNLW+YE
Sbjct: 3    VEKRPLQNSNAANVDSDPAGPKKTIEEMYQKKTQLEHILLRPDTYVGSIEKHTQNLWIYE 62

Query: 1029 NDKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYNGSGIS 850
            NDKMV+RS++YVPGLYKIFDEIL++AA+NK  DP MD LKV ID   N ISV+ NG G+ 
Sbjct: 63   NDKMVHRSVTYVPGLYKIFDEILVNAADNKQRDPSMDSLKVVIDAEQNCISVFNNGDGVP 122

Query: 849  VEINPETNVYLPEL---------NYSNFNYGLKGTRTGHGAKLTNIYSTEFIIETADGR- 700
            VE++ E  VY+PEL         NY +      G R G+GAKLTNI+STEF+IETADGR 
Sbjct: 123  VEVHQEEKVYVPELIFGHLLTSSNYDDTEKKTTGGRNGYGAKLTNIFSTEFVIETADGRR 182

Query: 699  -NKYKQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLMKKRVL 523
              KYKQVF++NM  KS P +TKCKE + WTKVTFKPDL KF M  L E+D+V LMKKRVL
Sbjct: 183  LKKYKQVFSDNMGKKSEPIITKCKEGDNWTKVTFKPDLAKFNMTHL-EDDVVALMKKRVL 241

Query: 522  DMAGFLGKTVKVELNGQ 472
            D+AG LGKTVKVELNGQ
Sbjct: 242  DLAGCLGKTVKVELNGQ 258



 Score =  166 bits (419), Expect = 1e-40
 Identities = 91/142 (64%), Positives = 101/142 (71%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            DE     IELAF KKK ++RK W+R+ +  T  +D+ +KLI +  F NKE  LFS ADLQ
Sbjct: 639  DEHDGEAIELAFSKKKIEERKNWLRQFEAGTH-LDQSEKLIKYSDFVNKELILFSMADLQ 697

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI          K   V QF GYVS HSAY HHG QSLASTIIGM
Sbjct: 698  RSIPSMVDGLKPGQRKILFCSFKRNFIKEAKVSQFSGYVSEHSAY-HHGEQSLASTIIGM 756

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVGSNNINLL PNGQFGTR
Sbjct: 757  AQDFVGSNNINLLLPNGQFGTR 778


>ref|XP_011621441.1| DNA topoisomerase 2 isoform X2 [Amborella trichopoda]
          Length = 1504

 Score =  273 bits (698), Expect = 1e-77
 Identities = 157/285 (55%), Positives = 196/285 (68%), Gaps = 28/285 (9%)
 Frame = -1

Query: 1179 EKPPLETS-----NAANILS-------EKLEASNQRHLD---ILCFRTMFLGPMDERTQN 1045
            +K PLETS     NAAN+ +       + +E + Q+      IL     ++G +++ TQN
Sbjct: 6    KKKPLETSSNFNSNAANLTTINGSKPGKTIEETYQKKTQLEHILLRPDTYIGSVEKHTQN 65

Query: 1044 LWVYENDKMVYRSISYVPGLYKIFDEILLDAAENKHSDPKMDFLKVSIDPSTNTISVYYN 865
            LWVYE +KMV+RSISYVPGLYKIFDEIL++AA+NK  DP MD LKV ID   NT+SVY N
Sbjct: 66   LWVYEENKMVHRSISYVPGLYKIFDEILVNAADNKQRDPSMDSLKVDIDVEQNTVSVYNN 125

Query: 864  GSGISVEINPETNVYLPE------LNYSNFNYGLK---GTRTGHGAKLTNIYSTEFIIET 712
            G G+ VEI+ E  VY+PE      L  SN+N  +K   G R G+GAKLTNI+ST+F+IET
Sbjct: 126  GDGVPVEIHQEEGVYVPEMIFGHLLTSSNYNDEVKKTTGGRNGYGAKLTNIFSTQFVIET 185

Query: 711  ADGR--NKYKQVFTNNMFDKSVPEVTKCKEDEIWTKVTFKPDLKKFGMDSLKEEDIVGLM 538
            ADG+   KYKQVFT+NM  KS P +TKCK  E WTKVTFKPDL KF M  L EED+V LM
Sbjct: 186  ADGKRQKKYKQVFTSNMGSKSEPAITKCKAGENWTKVTFKPDLAKFNMTYL-EEDVVALM 244

Query: 537  KKRVLDMAGFLGKTVKVELNGQLFRIIKSCD--DLNMKSDEKSKP 409
            KKRV+D+AG LGKTVKVELNG+   +    D  DL +KS  +++P
Sbjct: 245  KKRVVDLAGCLGKTVKVELNGKRLPVKSFVDYVDLYVKSASEARP 289



 Score =  158 bits (400), Expect = 3e-38
 Identities = 85/142 (59%), Positives = 101/142 (71%)
 Frame = -1

Query: 426  DEKSKPVIELAFRKKKAKDRKTWIRKLQRYTFTIDREDKLINFKSFFNKEAKLFSRADLQ 247
            D++    IELAF KKK + RK W+R  +  T+ +D+++KLI +  F NKE  LFS ADLQ
Sbjct: 646  DDQDGDAIELAFSKKKIEARKNWLRHFEPGTY-LDQKEKLIKYSDFVNKELILFSMADLQ 704

Query: 246  RSIPSLVDGLKLGQRKIXXXXXXXXXXKAINVGQFIGYVSAHSAYHHHGGQSLASTIIGM 67
            RSIPS+VDGLK GQRKI              V QF GYVS HSAY HHG QSLA+TI+GM
Sbjct: 705  RSIPSMVDGLKPGQRKILFCSFKRNFVSQAKVVQFSGYVSEHSAY-HHGEQSLAATIVGM 763

Query: 66   AQDFVGSNNINLLKPNGQFGTR 1
            AQDFVG+NNINLL+P GQFGTR
Sbjct: 764  AQDFVGTNNINLLQPLGQFGTR 785