BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00004991
         (1002 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004499342.1| PREDICTED: subtilisin-like protease SBT5.4 [...   158   1e-63
gb|AIC80771.1| subtilase [Cicer arietinum]                            158   1e-63
ref|XP_013458683.1| subtilisin-like serine protease [Medicago tr...   142   2e-59
gb|PNY02043.1| subtilisin-like protease-like protein, partial [T...   143   2e-59
ref|XP_003625338.1| subtilisin-like serine protease [Medicago tr...   138   3e-57
ref|XP_020230724.1| subtilisin-like protease SBT5.4 [Cajanus cajan]   142   8e-57
gb|KYP52120.1| Subtilisin-like protease [Cajanus cajan]               142   8e-57
ref|XP_003528716.1| PREDICTED: subtilisin-like protease SBT5.4 [...   136   2e-55
gb|KHN40225.1| Subtilisin-like protease [Glycine soja]                136   2e-55
gb|KRH51226.1| hypothetical protein GLYMA_07G269700 [Glycine max...   136   3e-55
ref|XP_015947728.1| subtilisin-like protease SBT5.4 [Arachis dur...   130   7e-52
ref|XP_018827964.1| PREDICTED: subtilisin-like protease SBT5.4 [...   124   5e-51
gb|PON88007.1| Subtilase [Trema orientalis]                           130   1e-50
ref|XP_023926000.1| subtilisin-like protease SBT5.4 [Quercus suber]   124   1e-50
gb|POE93750.1| subtilisin-like protease sbt5.4 [Quercus suber]        124   1e-50
ref|XP_018837366.1| PREDICTED: subtilisin-like protease SBT5.4 [...   124   2e-50
ref|XP_008384293.1| PREDICTED: LOW QUALITY PROTEIN: subtilisin-l...   118   5e-50
ref|XP_002269456.3| PREDICTED: subtilisin-like protease SBT5.4, ...   114   5e-50
ref|XP_016174125.1| subtilisin-like protease SBT5.4 [Arachis ipa...   124   6e-50
ref|XP_020418597.1| subtilisin-like protease SBT5.4 [Prunus pers...   126   8e-50

>ref|XP_004499342.1| PREDICTED: subtilisin-like protease SBT5.4 [Cicer arietinum]
          Length = 768

 Score =  158 bits (399), Expect(2) = 1e-63
 Identities = 89/172 (51%), Positives = 107/172 (62%), Gaps = 8/172 (4%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNY--- 681
            TRDNT KP+LD NN KATPFAYGSGH+R NRAMD GLVY+L INDYLNFLC LGYN    
Sbjct: 594  TRDNTAKPILDNNNMKATPFAYGSGHIRPNRAMDPGLVYELNINDYLNFLCFLGYNKTQI 653

Query: 682  -----NHINCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFV 846
                 NH +C  + ++     +  +        L+   + V P      V   R P+   
Sbjct: 654  KMFSGNHYHCHDINILDFNYPSITVPKLYGSVTLTRKLKNVGPPG--TYVASLRVPKGL- 710

Query: 847  YR*DFPYLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGKHY 1002
                  +++V+PNILKFD+IGEEKSFKV IE  +S VATVFG L WSDGKHY
Sbjct: 711  ------FIFVEPNILKFDNIGEEKSFKVIIEVARSGVATVFGGLTWSDGKHY 756



 Score =  115 bits (287), Expect(2) = 1e-63
 Identities = 55/75 (73%), Positives = 64/75 (85%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPGV+IIAA+S AVS +  +FD+ RVPF+TMSGTSMSCPHV G+VGLLKT HP 
Sbjct: 519 LKPDVTAPGVNIIAAYSGAVSPTEMEFDKRRVPFITMSGTSMSCPHVAGIVGLLKTLHPD 578

Query: 470 WSPSTIKSAIMKTGR 514
           WSPS IKSAIM T R
Sbjct: 579 WSPSAIKSAIMTTAR 593


>gb|AIC80771.1| subtilase [Cicer arietinum]
          Length = 757

 Score =  158 bits (399), Expect(2) = 1e-63
 Identities = 89/172 (51%), Positives = 107/172 (62%), Gaps = 8/172 (4%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNY--- 681
            TRDNT KP+LD NN KATPFAYGSGH+R NRAMD GLVY+L INDYLNFLC LGYN    
Sbjct: 583  TRDNTAKPILDNNNMKATPFAYGSGHIRPNRAMDPGLVYELNINDYLNFLCFLGYNKTQI 642

Query: 682  -----NHINCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFV 846
                 NH +C  + ++     +  +        L+   + V P      V   R P+   
Sbjct: 643  KMFSGNHYHCHDINILDFNYPSITVPKLYGSVTLTRKLKNVGPPG--TYVASLRVPKGL- 699

Query: 847  YR*DFPYLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGKHY 1002
                  +++V+PNILKFD+IGEEKSFKV IE  +S VATVFG L WSDGKHY
Sbjct: 700  ------FIFVEPNILKFDNIGEEKSFKVIIEVARSGVATVFGGLTWSDGKHY 745



 Score =  115 bits (287), Expect(2) = 1e-63
 Identities = 55/75 (73%), Positives = 64/75 (85%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPGV+IIAA+S AVS +  +FD+ RVPF+TMSGTSMSCPHV G+VGLLKT HP 
Sbjct: 508 LKPDVTAPGVNIIAAYSGAVSPTEMEFDKRRVPFITMSGTSMSCPHVAGIVGLLKTLHPD 567

Query: 470 WSPSTIKSAIMKTGR 514
           WSPS IKSAIM T R
Sbjct: 568 WSPSAIKSAIMTTAR 582


>ref|XP_013458683.1| subtilisin-like serine protease [Medicago truncatula]
 gb|KEH32715.1| subtilisin-like serine protease [Medicago truncatula]
          Length = 770

 Score =  142 bits (357), Expect(2) = 2e-59
 Identities = 86/172 (50%), Positives = 98/172 (56%), Gaps = 8/172 (4%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHI 690
            TRDNT KP+LD  N KATPFAYGSGH+R NRAMD GLVY+L INDYLNFLC LGYN   I
Sbjct: 596  TRDNTHKPILDDTNVKATPFAYGSGHIRPNRAMDPGLVYELNINDYLNFLCFLGYNQAQI 655

Query: 691  --------NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFV 846
                    +C  + ++     T  I        LS   + V P          R P    
Sbjct: 656  TMFSGTPYHCEDINILDFNYPTITIPKLYGSVTLSRKLKNVGPPG--TYTASLRVPVGL- 712

Query: 847  YR*DFPYLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGKHY 1002
                   + VQP  LKFD IGEEKSF +TIE T+S +ATVFG L WSDG HY
Sbjct: 713  ------SISVQPKKLKFDKIGEEKSFSLTIEVTRSGMATVFGGLTWSDGTHY 758



 Score =  117 bits (293), Expect(2) = 2e-59
 Identities = 56/75 (74%), Positives = 65/75 (86%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPGV+IIAA+S A+S +  DFD+ RVPF+TMSGTSMSCPHV GVVGLLKT HP+
Sbjct: 521 LKPDVTAPGVNIIAAYSGAISPTELDFDKRRVPFITMSGTSMSCPHVAGVVGLLKTLHPN 580

Query: 470 WSPSTIKSAIMKTGR 514
           WSPS IKSAIM T R
Sbjct: 581 WSPSAIKSAIMTTAR 595


>gb|PNY02043.1| subtilisin-like protease-like protein, partial [Trifolium pratense]
          Length = 581

 Score =  143 bits (360), Expect(2) = 2e-59
 Identities = 84/172 (48%), Positives = 99/172 (57%), Gaps = 8/172 (4%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNY--- 681
            TR N  KP+LD NN KATPFAYGSGH+R NRAMD GLVY+L INDYLNFLC +GYN    
Sbjct: 407  TRANNAKPILDDNNVKATPFAYGSGHIRPNRAMDPGLVYELNINDYLNFLCFIGYNQTQI 466

Query: 682  -----NHINCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFV 846
                  H +C  + ++     T  I        LS   + V P          R P    
Sbjct: 467  SMFSGTHYHCKDINILDFNYPTITIPQLYGSVTLSRKLKNVGPPG--TYKATLRVPAGV- 523

Query: 847  YR*DFPYLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGKHY 1002
                   + VQPNILKFD IG+EKSF +T+E  +S +ATVFG L WSDGKHY
Sbjct: 524  ------SVSVQPNILKFDKIGDEKSFNLTVEVKRSGLATVFGGLTWSDGKHY 569



 Score =  115 bits (289), Expect(2) = 2e-59
 Identities = 55/75 (73%), Positives = 63/75 (84%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PDITAPGV+I+AA+S  +S S  DFD+ R+PF+TMSGTSMSCPHV GVVGLLKT HP 
Sbjct: 332 LKPDITAPGVNIVAAYSGGISPSEMDFDKRRIPFITMSGTSMSCPHVAGVVGLLKTLHPD 391

Query: 470 WSPSTIKSAIMKTGR 514
           WSPS IKSAIM T R
Sbjct: 392 WSPSAIKSAIMTTAR 406


>ref|XP_003625338.1| subtilisin-like serine protease [Medicago truncatula]
 gb|AES81556.1| subtilisin-like serine protease [Medicago truncatula]
          Length = 766

 Score =  138 bits (348), Expect(2) = 3e-57
 Identities = 84/172 (48%), Positives = 98/172 (56%), Gaps = 8/172 (4%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHI 690
            TRDNT KP++D  N KATPF YGSGH+R NRAMD GLVY+L INDY+NFLC LGYN   I
Sbjct: 592  TRDNTVKPIVDDINVKATPFDYGSGHIRPNRAMDPGLVYELNINDYINFLCFLGYNQTQI 651

Query: 691  --------NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFV 846
                    +C  + ++     T  I I      LS   + V P          R P    
Sbjct: 652  SMFSGTNHHCDGINILDFNYPTITIPILYGSVTLSRKLKNVGPPG--TYTASLRVPAGL- 708

Query: 847  YR*DFPYLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGKHY 1002
                   + VQP  LKFD IGEEKSF +TIE T+S  ATVFG L WSDGKH+
Sbjct: 709  ------SISVQPKKLKFDKIGEEKSFNLTIEVTRSGGATVFGGLTWSDGKHH 754



 Score =  113 bits (283), Expect(2) = 3e-57
 Identities = 56/75 (74%), Positives = 63/75 (84%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPGV+IIAA+S AVS +  D D+ RVPF+TMSGTSMSCPHV GVVGLLKT HP 
Sbjct: 517 LKPDVTAPGVNIIAAYSGAVSPTKLDSDKRRVPFMTMSGTSMSCPHVAGVVGLLKTLHPT 576

Query: 470 WSPSTIKSAIMKTGR 514
           WSPS IKSAIM T R
Sbjct: 577 WSPSAIKSAIMTTAR 591


>ref|XP_020230724.1| subtilisin-like protease SBT5.4 [Cajanus cajan]
          Length = 771

 Score =  142 bits (358), Expect(2) = 8e-57
 Identities = 83/174 (47%), Positives = 104/174 (59%), Gaps = 11/174 (6%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHI 690
            TRDNTGKPMLD N+ KATPFAYGSGH+R NRAMD GLVYDL  NDYLNFLC  GYN+  I
Sbjct: 596  TRDNTGKPMLDGNSEKATPFAYGSGHIRPNRAMDPGLVYDLNNNDYLNFLCVTGYNHTQI 655

Query: 691  --------NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFV 846
                    +C  ++ I+     S I+I +    +S   R          V    +P  ++
Sbjct: 656  KAFSGAPYHCPDVIRILDFNYPS-ITIPVLYGSVSLTRR----------VKNVGSPGTYI 704

Query: 847  YR*DFP---YLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGKH 999
             R   P    + V+P  LKFD IGEEKS+K+T+E  +  V T FGR+ WSDG+H
Sbjct: 705  ARLKAPEGVSVIVEPGKLKFDKIGEEKSYKLTVEVERPGVGTSFGRITWSDGRH 758



 Score =  108 bits (269), Expect(2) = 8e-57
 Identities = 51/75 (68%), Positives = 61/75 (81%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPGV+IIAA+S  +S +   FD+ R+PF+TMSGTSMSCPHV GVV LLKT HP 
Sbjct: 521 LKPDVTAPGVNIIAAYSEGISPTEMSFDKRRIPFITMSGTSMSCPHVAGVVSLLKTLHPD 580

Query: 470 WSPSTIKSAIMKTGR 514
           WSP+ IKSAIM T R
Sbjct: 581 WSPAAIKSAIMTTTR 595


>gb|KYP52120.1| Subtilisin-like protease [Cajanus cajan]
          Length = 737

 Score =  142 bits (358), Expect(2) = 8e-57
 Identities = 83/174 (47%), Positives = 104/174 (59%), Gaps = 11/174 (6%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHI 690
            TRDNTGKPMLD N+ KATPFAYGSGH+R NRAMD GLVYDL  NDYLNFLC  GYN+  I
Sbjct: 562  TRDNTGKPMLDGNSEKATPFAYGSGHIRPNRAMDPGLVYDLNNNDYLNFLCVTGYNHTQI 621

Query: 691  --------NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFV 846
                    +C  ++ I+     S I+I +    +S   R          V    +P  ++
Sbjct: 622  KAFSGAPYHCPDVIRILDFNYPS-ITIPVLYGSVSLTRR----------VKNVGSPGTYI 670

Query: 847  YR*DFP---YLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGKH 999
             R   P    + V+P  LKFD IGEEKS+K+T+E  +  V T FGR+ WSDG+H
Sbjct: 671  ARLKAPEGVSVIVEPGKLKFDKIGEEKSYKLTVEVERPGVGTSFGRITWSDGRH 724



 Score =  108 bits (269), Expect(2) = 8e-57
 Identities = 51/75 (68%), Positives = 61/75 (81%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPGV+IIAA+S  +S +   FD+ R+PF+TMSGTSMSCPHV GVV LLKT HP 
Sbjct: 487 LKPDVTAPGVNIIAAYSEGISPTEMSFDKRRIPFITMSGTSMSCPHVAGVVSLLKTLHPD 546

Query: 470 WSPSTIKSAIMKTGR 514
           WSP+ IKSAIM T R
Sbjct: 547 WSPAAIKSAIMTTTR 561


>ref|XP_003528716.1| PREDICTED: subtilisin-like protease SBT5.4 [Glycine max]
 gb|KRH51225.1| hypothetical protein GLYMA_07G269700 [Glycine max]
          Length = 773

 Score =  136 bits (342), Expect(2) = 2e-55
 Identities = 84/174 (48%), Positives = 104/174 (59%), Gaps = 12/174 (6%)
 Frame = +1

Query: 511  TRDNTGKPMLDF-NNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNH 687
            TRDNTGKPMLD  N+AKATPFAYGSGH+R NRAMD GLVYDLT NDYLNFLC   YN + 
Sbjct: 597  TRDNTGKPMLDGGNDAKATPFAYGSGHIRPNRAMDPGLVYDLTNNDYLNFLCFSIYNQSQ 656

Query: 688  I--------NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAF 843
            I         C  ++ I+     ++ +IT+         +L    S    V     P  +
Sbjct: 657  IEMFNGARYRCPDIINILD---FNYPTITI--------PKLYGSVSVTRRVKNVGPPGTY 705

Query: 844  VYR*DFP---YLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGK 996
              R   P    + V+PN+LKFD+IGEEKSFK+T+E T+    T FG + WSDGK
Sbjct: 706  TARLKVPARLSISVEPNVLKFDNIGEEKSFKLTVEVTRPGETTAFGGITWSDGK 759



 Score =  109 bits (272), Expect(2) = 2e-55
 Identities = 52/75 (69%), Positives = 61/75 (81%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+ APGV+IIAA+S  VS +   FD+ RVPF+TMSGTSMSCPHV GVVGLLKT HP 
Sbjct: 522 LKPDVIAPGVNIIAAYSEGVSPTNLGFDKRRVPFITMSGTSMSCPHVAGVVGLLKTLHPD 581

Query: 470 WSPSTIKSAIMKTGR 514
           WSP+ IKSA+M T R
Sbjct: 582 WSPAVIKSALMTTAR 596


>gb|KHN40225.1| Subtilisin-like protease [Glycine soja]
          Length = 739

 Score =  136 bits (342), Expect(2) = 2e-55
 Identities = 84/174 (48%), Positives = 104/174 (59%), Gaps = 12/174 (6%)
 Frame = +1

Query: 511  TRDNTGKPMLDF-NNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNH 687
            TRDNTGKPMLD  N+AKATPFAYGSGH+R NRAMD GLVYDLT NDYLNFLC   YN + 
Sbjct: 563  TRDNTGKPMLDGGNDAKATPFAYGSGHIRPNRAMDPGLVYDLTNNDYLNFLCFSIYNQSQ 622

Query: 688  I--------NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAF 843
            I         C  ++ I+     ++ +IT+         +L    S    V     P  +
Sbjct: 623  IEMFNGARYRCPDIINILD---FNYPTITI--------PKLYGSVSVTRRVKNVGPPGTY 671

Query: 844  VYR*DFP---YLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGK 996
              R   P    + V+PN+LKFD+IGEEKSFK+T+E T+    T FG + WSDGK
Sbjct: 672  TARLKVPARLSISVEPNVLKFDNIGEEKSFKLTVEVTRPGETTAFGGITWSDGK 725



 Score =  109 bits (272), Expect(2) = 2e-55
 Identities = 52/75 (69%), Positives = 61/75 (81%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+ APGV+IIAA+S  VS +   FD+ RVPF+TMSGTSMSCPHV GVVGLLKT HP 
Sbjct: 488 LKPDVIAPGVNIIAAYSEGVSPTNLGFDKRRVPFITMSGTSMSCPHVAGVVGLLKTLHPD 547

Query: 470 WSPSTIKSAIMKTGR 514
           WSP+ IKSA+M T R
Sbjct: 548 WSPAVIKSALMTTAR 562


>gb|KRH51226.1| hypothetical protein GLYMA_07G269700 [Glycine max]
 gb|KRH51227.1| hypothetical protein GLYMA_07G269700 [Glycine max]
          Length = 606

 Score =  136 bits (342), Expect(2) = 3e-55
 Identities = 84/174 (48%), Positives = 104/174 (59%), Gaps = 12/174 (6%)
 Frame = +1

Query: 511 TRDNTGKPMLDF-NNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNH 687
           TRDNTGKPMLD  N+AKATPFAYGSGH+R NRAMD GLVYDLT NDYLNFLC   YN + 
Sbjct: 430 TRDNTGKPMLDGGNDAKATPFAYGSGHIRPNRAMDPGLVYDLTNNDYLNFLCFSIYNQSQ 489

Query: 688 I--------NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAF 843
           I         C  ++ I+     ++ +IT+         +L    S    V     P  +
Sbjct: 490 IEMFNGARYRCPDIINILD---FNYPTITI--------PKLYGSVSVTRRVKNVGPPGTY 538

Query: 844 VYR*DFP---YLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGK 996
             R   P    + V+PN+LKFD+IGEEKSFK+T+E T+    T FG + WSDGK
Sbjct: 539 TARLKVPARLSISVEPNVLKFDNIGEEKSFKLTVEVTRPGETTAFGGITWSDGK 592



 Score =  109 bits (272), Expect(2) = 3e-55
 Identities = 52/75 (69%), Positives = 61/75 (81%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+ APGV+IIAA+S  VS +   FD+ RVPF+TMSGTSMSCPHV GVVGLLKT HP 
Sbjct: 355 LKPDVIAPGVNIIAAYSEGVSPTNLGFDKRRVPFITMSGTSMSCPHVAGVVGLLKTLHPD 414

Query: 470 WSPSTIKSAIMKTGR 514
           WSP+ IKSA+M T R
Sbjct: 415 WSPAVIKSALMTTAR 429


>ref|XP_015947728.1| subtilisin-like protease SBT5.4 [Arachis duranensis]
          Length = 778

 Score =  130 bits (326), Expect(2) = 7e-52
 Identities = 83/180 (46%), Positives = 97/180 (53%), Gaps = 16/180 (8%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHI 690
            TRDN G+P+LD +   ATPF YG GHVR NRAMD GLVYD TI+DYLNFLCA+GY+   I
Sbjct: 601  TRDNKGEPILDGDFKSATPFEYGFGHVRPNRAMDPGLVYDTTIDDYLNFLCAIGYDQKRI 660

Query: 691  NCSILLLIVVKT*TSWISITLE*QCLSFMARL--V*PES*KMLVHQARTPQAFVYR*DFP 864
                             S  L  +C   M+ L    P     +++   T    V     P
Sbjct: 661  KV--------------FSGALHHECPHGMSVLDFNYPSITVPVLYGRVTVTRRVRNVGSP 706

Query: 865  YLY--------------VQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGKHY 1002
              Y              V PN+LKF+S+GEEKSFKVTIE  K   A VFG LIWSDGKHY
Sbjct: 707  GTYVASFHNIPSGLSLSVNPNVLKFESVGEEKSFKVTIEVAKPGRAIVFGDLIWSDGKHY 766



 Score =  103 bits (258), Expect(2) = 7e-52
 Identities = 50/77 (64%), Positives = 60/77 (77%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPG++IIAAFS A S +   FD+ R PF+ +SGTSMSCPHV G+ GLLKT HP 
Sbjct: 526 LKPDVTAPGINIIAAFSEAASPTQMSFDKRRAPFVALSGTSMSCPHVAGIAGLLKTLHPE 585

Query: 470 WSPSTIKSAIMKTGRGT 520
           WSPS IKSAI+ T  GT
Sbjct: 586 WSPSAIKSAIL-TSAGT 601


>ref|XP_018827964.1| PREDICTED: subtilisin-like protease SBT5.4 [Juglans regia]
          Length = 783

 Score =  124 bits (311), Expect(2) = 5e-51
 Identities = 74/166 (44%), Positives = 96/166 (57%), Gaps = 2/166 (1%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHI 690
            TRDNT  P+L+ +  KATPF+YG+GH+R NRAMD GLVYDLT+NDYL+FLCALGY    +
Sbjct: 603  TRDNTVHPVLNGSFKKATPFSYGAGHIRPNRAMDPGLVYDLTVNDYLDFLCALGYKQTEV 662

Query: 691  NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFVYR*DFPYL 870
                          S +        +  ++  V        V    T  A V +     +
Sbjct: 663  ELFSEAPYECPKSASLLDFNNPSIAVPKLSGSVTVTRKLKNVGSPGTYSARVRKPAGISI 722

Query: 871  YVQPNILKFDSIGEEKSFKVTIEATKSSVAT--VFGRLIWSDGKHY 1002
             V+PN LKF+ +GEEKSFK+T+++ K  VA   VFG L WSDGKHY
Sbjct: 723  LVEPNTLKFEKVGEEKSFKLTLKSDKPGVAKGYVFGGLTWSDGKHY 768



 Score =  107 bits (266), Expect(2) = 5e-51
 Identities = 54/75 (72%), Positives = 58/75 (77%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PDITAPGVDIIA F+ A S S   FD  RV F TMSGTSMSCPHV GVVGLLKT HP 
Sbjct: 528 LKPDITAPGVDIIAGFTGATSPSNLPFDNRRVTFNTMSGTSMSCPHVSGVVGLLKTLHPD 587

Query: 470 WSPSTIKSAIMKTGR 514
           WSP+ I+SAIM T R
Sbjct: 588 WSPAAIRSAIMTTAR 602


>gb|PON88007.1| Subtilase [Trema orientalis]
          Length = 766

 Score =  130 bits (327), Expect(2) = 1e-50
 Identities = 81/177 (45%), Positives = 105/177 (59%), Gaps = 13/177 (7%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHI 690
            TRDNT +P+L+ +N KATPF+YG+GHV+ NRAMD GLVYDL+INDYL+FLC+LGYN   I
Sbjct: 591  TRDNTMEPILNASNFKATPFSYGAGHVQPNRAMDPGLVYDLSINDYLSFLCSLGYNATQI 650

Query: 691  --------NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFV 846
                     CS  + +   T  ++ SIT+     S              +    TP  + 
Sbjct: 651  EMFSEEPYRCSKNIRL---TNLNYPSITVPNLSGSITV--------TRTLKNVGTPGTYR 699

Query: 847  YR*DFP---YLYVQPNILKFDSIGEEKSFKVTIEATKSSVAT--VFGRLIWSDGKHY 1002
             R   P    + V+PN L+F  +GEEKSFKVT++  K   A   VFG+LIWSDGKHY
Sbjct: 700  ARVQDPAGIVISVKPNYLRFKRVGEEKSFKVTLKVKKPKAARDYVFGKLIWSDGKHY 756



 Score = 99.8 bits (247), Expect(2) = 1e-50
 Identities = 48/75 (64%), Positives = 58/75 (77%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PDITAPGV +IAA+S A   +   FD+ RVPF ++SGTSMSCPHV G+ GLLKT HP 
Sbjct: 516 LKPDITAPGVSVIAAYSEAQGPTNQVFDKRRVPFNSVSGTSMSCPHVSGIAGLLKTLHPD 575

Query: 470 WSPSTIKSAIMKTGR 514
           WSP+ IKSAIM + R
Sbjct: 576 WSPAAIKSAIMTSAR 590


>ref|XP_023926000.1| subtilisin-like protease SBT5.4 [Quercus suber]
          Length = 755

 Score =  124 bits (312), Expect(2) = 1e-50
 Identities = 72/169 (42%), Positives = 100/169 (59%), Gaps = 6/169 (3%)
 Frame = +1

Query: 514  RDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHIN 693
            RDNT  P+LD +  KATPF+YG+GH+R NRAMD GLVYDLT+NDYL+FLCA GYN   + 
Sbjct: 577  RDNTKSPILDGSFIKATPFSYGAGHIRPNRAMDPGLVYDLTVNDYLDFLCASGYNQTLLE 636

Query: 694  CSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFVYR*DFPY-- 867
                         S +        ++ ++R V   +    +    +P  ++ R   P   
Sbjct: 637  LFTEAPYKCPKSASLLDFNYPSITVTNLSRSV---NVTRKLKNVGSPGTYLARVRGPIGI 693

Query: 868  -LYVQPNILKFDSIGEEKSFKVTIEATKSSVAT---VFGRLIWSDGKHY 1002
             ++V+P ILKF+SIGEEKSFK+T+    + + T   VFG ++WSDG HY
Sbjct: 694  SVHVEPKILKFESIGEEKSFKLTVTKKTAGMGTEDYVFGVIVWSDGNHY 742



 Score =  105 bits (261), Expect(2) = 1e-50
 Identities = 50/75 (66%), Positives = 60/75 (80%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPGV+IIAA++   S +  +FD  R+PF+TMSGTSMSCPHV GVVGLLK  HP 
Sbjct: 501 LKPDVTAPGVNIIAAYTGEASPTEENFDTRRIPFITMSGTSMSCPHVSGVVGLLKKLHPD 560

Query: 470 WSPSTIKSAIMKTGR 514
           WSPS IKSA+M T R
Sbjct: 561 WSPSAIKSALMTTAR 575


>gb|POE93750.1| subtilisin-like protease sbt5.4 [Quercus suber]
          Length = 615

 Score =  124 bits (312), Expect(2) = 1e-50
 Identities = 72/169 (42%), Positives = 100/169 (59%), Gaps = 6/169 (3%)
 Frame = +1

Query: 514  RDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHIN 693
            RDNT  P+LD +  KATPF+YG+GH+R NRAMD GLVYDLT+NDYL+FLCA GYN   + 
Sbjct: 437  RDNTKSPILDGSFIKATPFSYGAGHIRPNRAMDPGLVYDLTVNDYLDFLCASGYNQTLLE 496

Query: 694  CSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFVYR*DFPY-- 867
                         S +        ++ ++R V   +    +    +P  ++ R   P   
Sbjct: 497  LFTEAPYKCPKSASLLDFNYPSITVTNLSRSV---NVTRKLKNVGSPGTYLARVRGPIGI 553

Query: 868  -LYVQPNILKFDSIGEEKSFKVTIEATKSSVAT---VFGRLIWSDGKHY 1002
             ++V+P ILKF+SIGEEKSFK+T+    + + T   VFG ++WSDG HY
Sbjct: 554  SVHVEPKILKFESIGEEKSFKLTVTKKTAGMGTEDYVFGVIVWSDGNHY 602



 Score =  105 bits (261), Expect(2) = 1e-50
 Identities = 50/75 (66%), Positives = 60/75 (80%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPGV+IIAA++   S +  +FD  R+PF+TMSGTSMSCPHV GVVGLLK  HP 
Sbjct: 361 LKPDVTAPGVNIIAAYTGEASPTEENFDTRRIPFITMSGTSMSCPHVSGVVGLLKKLHPD 420

Query: 470 WSPSTIKSAIMKTGR 514
           WSPS IKSA+M T R
Sbjct: 421 WSPSAIKSALMTTAR 435


>ref|XP_018837366.1| PREDICTED: subtilisin-like protease SBT5.4 [Juglans regia]
          Length = 775

 Score =  124 bits (310), Expect(2) = 2e-50
 Identities = 73/169 (43%), Positives = 102/169 (60%), Gaps = 5/169 (2%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHI 690
            TRDNT KPML+ +  KATPF  G+GH+R NRAMD GLVYDLT +DYLNFLC+LGYN   +
Sbjct: 592  TRDNTVKPMLNASYVKATPFEQGAGHIRPNRAMDPGLVYDLTNDDYLNFLCSLGYNQTIV 651

Query: 691  NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAFVYR*DFPY- 867
                 +        S +        +  ++  V   +    +    +P  +  R   P  
Sbjct: 652  ELFSDVTYECPKSASLLDFNYPSIAVPKLSGKV---TLTRKLKNVGSPGIYAARVLNPVG 708

Query: 868  --LYVQPNILKFDSIGEEKSFKVTIEATKSSVAT--VFGRLIWSDGKHY 1002
              ++V+P+IL+F+++GEEKSF +T+EA +S +A   VFG LIWSDG+HY
Sbjct: 709  VSVHVEPHILQFENVGEEKSFTLTLEAEESGLARDYVFGELIWSDGRHY 757



 Score =  105 bits (261), Expect(2) = 2e-50
 Identities = 51/75 (68%), Positives = 58/75 (77%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPGVDIIA F+ A+S +   FD  RVPF  MSGTSMSCPHV GV GLLKT HP 
Sbjct: 517 LKPDVTAPGVDIIAGFTQAISPTELPFDNRRVPFNIMSGTSMSCPHVSGVAGLLKTLHPS 576

Query: 470 WSPSTIKSAIMKTGR 514
           WSP+ I+SAIM T R
Sbjct: 577 WSPAAIRSAIMTTAR 591


>ref|XP_008384293.1| PREDICTED: LOW QUALITY PROTEIN: subtilisin-like protease SBT5.4
            [Malus domestica]
          Length = 775

 Score =  118 bits (295), Expect(2) = 5e-50
 Identities = 77/178 (43%), Positives = 101/178 (56%), Gaps = 14/178 (7%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHI 690
            TRDNT  PM+  +  +ATPF+YGSGH+R NRAMD GL+YDLT NDYL+FLCA+GYN   I
Sbjct: 593  TRDNTXNPMMSASFVEATPFSYGSGHIRPNRAMDPGLIYDLTANDYLDFLCAIGYNKTTI 652

Query: 691  ---------NCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAF 843
                      C     ++     S IS+      ++   RL         V    T    
Sbjct: 653  QLFSEGPNYKCPESASLLDFNYPS-ISVPRLSGSVTVTRRL-------KNVGSPGTYAVR 704

Query: 844  VYR*DFPYLYVQPNILKFDSIGEEKSFKVTIEA--TKSSVAT---VFGRLIWSDGKHY 1002
            + R +   + V+PN+L+F S+GEEKSF+VT++A   +  VA    VFG L WSDGKHY
Sbjct: 705  IKRANGISVSVEPNVLRFKSVGEEKSFRVTLKAKPKRGGVAKGDYVFGGLTWSDGKHY 762



 Score =  109 bits (273), Expect(2) = 5e-50
 Identities = 54/75 (72%), Positives = 62/75 (82%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PDITAPGV+IIAA++ A S +  DFD+ RVPF T SGTSMSCPHV GVVGLLKT +PH
Sbjct: 518 LKPDITAPGVNIIAAYTQATSPTDQDFDKRRVPFNTESGTSMSCPHVSGVVGLLKTLYPH 577

Query: 470 WSPSTIKSAIMKTGR 514
           WSPS I+SAIM T R
Sbjct: 578 WSPSAIRSAIMTTAR 592


>ref|XP_002269456.3| PREDICTED: subtilisin-like protease SBT5.4, partial [Vitis vinifera]
          Length = 578

 Score =  114 bits (285), Expect(2) = 5e-50
 Identities = 75/182 (41%), Positives = 101/182 (55%), Gaps = 18/182 (9%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNY--- 681
            T DNT  PM D ++ KATP AYG+GH++ N+A D GLVYDLT+NDYL+FLCALGYN    
Sbjct: 402  TSDNTKSPMKDSSSDKATPLAYGAGHMQPNQAADPGLVYDLTVNDYLDFLCALGYNQTML 461

Query: 682  -----NHINC--SILLL------IVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQ 822
                 N   C  S+ LL      I V   +  +++T   + + F             ++ 
Sbjct: 462  KAFSDNPYKCPASVSLLDFNYPSITVPNLSGSVTLTRRVKNVGFPG-----------IYA 510

Query: 823  ARTPQAFVYR*DFPYLYVQPNILKFDSIGEEKSFKVTIEATKSSVAT--VFGRLIWSDGK 996
            A   Q          + V+P+ILKF  IGEEK FKVT++A  +  A   VFG+LIW+D K
Sbjct: 511  AHISQPTGVS-----VTVEPSILKFSRIGEEKKFKVTLKANTNGEAKDYVFGQLIWTDDK 565

Query: 997  HY 1002
            H+
Sbjct: 566  HH 567



 Score =  113 bits (283), Expect(2) = 5e-50
 Identities = 55/73 (75%), Positives = 61/73 (83%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PDITAPGVDIIAAF+ A S +  DFDE R+PF ++SGTSMSCPHV GV GLLKT HPH
Sbjct: 327 LKPDITAPGVDIIAAFTEAQSPTEQDFDERRLPFYSLSGTSMSCPHVAGVAGLLKTIHPH 386

Query: 470 WSPSTIKSAIMKT 508
           WSPS IKSAIM T
Sbjct: 387 WSPSAIKSAIMTT 399


>ref|XP_016174125.1| subtilisin-like protease SBT5.4 [Arachis ipaensis]
          Length = 775

 Score =  124 bits (312), Expect(2) = 6e-50
 Identities = 80/177 (45%), Positives = 99/177 (55%), Gaps = 13/177 (7%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYN-- 684
            TRDN G+PMLD +   ATPF YG GHVR NRAMD GLVYD +I+DYLNFLC +GY+    
Sbjct: 598  TRDNKGEPMLDGDFKAATPFEYGFGHVRPNRAMDPGLVYDTSIDDYLNFLCTIGYDQKKI 657

Query: 685  -------HINCSILLLIVVKT*TSWISITLE*QCLSFMARLV*PES*KMLVHQARTPQAF 843
                   H  C   + ++     ++ SIT+         R+         V    +P  +
Sbjct: 658  KRFSGTPHHECPDGMGVLD---VNYPSITVP----VLYGRVTVTRR----VRNVGSPGTY 706

Query: 844  VYR-*DFP---YLYVQPNILKFDSIGEEKSFKVTIEATKSSVATVFGRLIWSDGKHY 1002
            V    D P    L V PN+LKF S+GEEKSFKVT+E  K   + VFG L WSDGKHY
Sbjct: 707  VASFNDIPSGLSLSVNPNVLKFQSVGEEKSFKVTMEVAKPGRSIVFGDLTWSDGKHY 763



 Score =  102 bits (255), Expect(2) = 6e-50
 Identities = 48/75 (64%), Positives = 57/75 (76%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PD+TAPGV+IIAAFS A S +   FD  + PF  +SGTSMSCPHV G+ GLLKT HP 
Sbjct: 523 LKPDVTAPGVNIIAAFSEAASPTEMSFDNRKAPFAALSGTSMSCPHVAGIAGLLKTLHPE 582

Query: 470 WSPSTIKSAIMKTGR 514
           WSPS IKSAI+ + R
Sbjct: 583 WSPSAIKSAILTSAR 597


>ref|XP_020418597.1| subtilisin-like protease SBT5.4 [Prunus persica]
 gb|ONI09648.1| hypothetical protein PRUPE_4G001800 [Prunus persica]
          Length = 774

 Score =  126 bits (317), Expect(2) = 8e-50
 Identities = 82/181 (45%), Positives = 103/181 (56%), Gaps = 17/181 (9%)
 Frame = +1

Query: 511  TRDNTGKPMLDFNNAKATPFAYGSGHVRLNRAMDHGLVYDLTINDYLNFLCALGYNYNHI 690
            TRDNT  PM + +  +ATPF+YG+GH+R NRAMD GL+YDLT+NDYL+FLCA+GYN    
Sbjct: 593  TRDNTANPMKNASFIEATPFSYGAGHIRPNRAMDPGLIYDLTVNDYLDFLCAIGYN---- 648

Query: 691  NCSILLLIVVKT*TSWISITLE*QC------LSF-MARLV*PE-----S*KMLVHQARTP 834
                      KT     S +   +C      L F    +V PE     +    V    +P
Sbjct: 649  ----------KTMMQLFSESPNYKCPKSASLLDFNYPSIVVPELSGSVTVTRRVKNVGSP 698

Query: 835  QAFVYR*DFPY---LYVQPNILKFDSIGEEKSFKVTIEATKSSVAT--VFGRLIWSDGKH 999
              +  R   P    + V+PNILKF +IGEEKSFKVT++A    V    VFG LIWSDGKH
Sbjct: 699  GTYAVRAHKPLGVSVTVEPNILKFKNIGEEKSFKVTLKAKGLGVTKDYVFGGLIWSDGKH 758

Query: 1000 Y 1002
            Y
Sbjct: 759  Y 759



 Score =  100 bits (249), Expect(2) = 8e-50
 Identities = 50/75 (66%), Positives = 59/75 (78%)
 Frame = +2

Query: 290 LQPDITAPGVDIIAAFS*AVSFSGYDFDECRVPFLTMSGTSMSCPHVVGVVGLLKTFHPH 469
           L+PDITAPGV+IIAA++ A S +   FD+ R+ F T SGTSMSCPHV GVVGLLKT +P 
Sbjct: 518 LKPDITAPGVNIIAAYTQATSPTNESFDKRRIAFNTESGTSMSCPHVSGVVGLLKTLYPD 577

Query: 470 WSPSTIKSAIMKTGR 514
           WSPS I+SAIM T R
Sbjct: 578 WSPSAIRSAIMTTAR 592