BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00004988
         (900 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium prate...    62   1e-09
ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arieti...    65   8e-08
sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName:...    63   4e-07
dbj|GAU12625.1| hypothetical protein TSUD_121160 [Trifolium subt...    61   1e-06
dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subt...    61   1e-06
ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago tru...    61   1e-06
dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subt...    59   6e-06

>gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium pratense]
          Length = 1492

 Score = 48.5 bits (114), Expect(2) = 1e-09
 Identities = 25/34 (73%), Positives = 28/34 (82%)
 Frame = +1

Query: 523  RNVGTKRTLGEKLLTDMLQPAEGTGTSPEKKVRK 624
            RNVGTK+  G+K+LTDMLQPAE    SPEKKVRK
Sbjct: 1371 RNVGTKQLSGQKILTDMLQPAE----SPEKKVRK 1400



 Score = 43.1 bits (100), Expect(2) = 1e-09
 Identities = 21/26 (80%), Positives = 22/26 (84%)
 Frame = +2

Query: 611  RK*GKMMESPFNKKSGSILGRAANKD 688
            +K  KM ESPFNKKSGSILGR ANKD
Sbjct: 1396 KKVRKMRESPFNKKSGSILGRDANKD 1421



 Score = 61.6 bits (148), Expect = 9e-07
 Identities = 31/37 (83%), Positives = 31/37 (83%)
 Frame = +2

Query: 788  KKVRKMRESPFNKKSGSILGRAANKDKRPSEDLSSGS 898
            KKVRKMRESPFNKKSGSILGR ANKD  P  DLS GS
Sbjct: 1396 KKVRKMRESPFNKKSGSILGRDANKDISPLADLSEGS 1432


>ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
 ref|XP_004517303.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
          Length = 1472

 Score = 64.7 bits (156), Expect = 8e-08
 Identities = 30/34 (88%), Positives = 33/34 (97%)
 Frame = +1

Query: 523  RNVGTKRTLGEKLLTDMLQPAEGTGTSPEKKVRK 624
            RNVG K+T+G+KLLTDMLQPAEGTGTSPEKKVRK
Sbjct: 1350 RNVGGKQTMGQKLLTDMLQPAEGTGTSPEKKVRK 1383



 Score = 64.3 bits (155), Expect = 1e-07
 Identities = 32/37 (86%), Positives = 33/37 (89%)
 Frame = +2

Query: 788  KKVRKMRESPFNKKSGSILGRAANKDKRPSEDLSSGS 898
            KKVRKMRESPFNKKSGSILGRAANKD  P  DLS+GS
Sbjct: 1379 KKVRKMRESPFNKKSGSILGRAANKDISPIADLSAGS 1415


>sp|O24308.1|TOP2_PEA RecName: Full=DNA topoisomerase 2; AltName: Full=DNA topoisomerase
            II; AltName: Full=PsTopII
 emb|CAA74891.1| topoisomerase II [Pisum sativum]
          Length = 1462

 Score = 62.8 bits (151), Expect = 4e-07
 Identities = 29/34 (85%), Positives = 33/34 (97%)
 Frame = +1

Query: 523  RNVGTKRTLGEKLLTDMLQPAEGTGTSPEKKVRK 624
            R+VGTK++ G+KLLTDMLQPAEGTGTSPEKKVRK
Sbjct: 1338 RSVGTKQSAGQKLLTDMLQPAEGTGTSPEKKVRK 1371


>dbj|GAU12625.1| hypothetical protein TSUD_121160 [Trifolium subterraneum]
          Length = 1315

 Score = 61.2 bits (147), Expect = 1e-06
 Identities = 29/34 (85%), Positives = 31/34 (91%)
 Frame = +1

Query: 523  RNVGTKRTLGEKLLTDMLQPAEGTGTSPEKKVRK 624
            RNVGTK+  G+KLLTDMLQPAE TGTSPEKKVRK
Sbjct: 1190 RNVGTKQLSGQKLLTDMLQPAESTGTSPEKKVRK 1223



 Score = 59.7 bits (143), Expect = 4e-06
 Identities = 29/37 (78%), Positives = 30/37 (81%)
 Frame = +2

Query: 788  KKVRKMRESPFNKKSGSILGRAANKDKRPSEDLSSGS 898
            KKVRKMRESPFNKKSGS+LGR ANKD  P  D S GS
Sbjct: 1219 KKVRKMRESPFNKKSGSVLGRDANKDISPLADFSEGS 1255


>dbj|GAU12623.1| hypothetical protein TSUD_121150 [Trifolium subterraneum]
          Length = 1359

 Score = 61.2 bits (147), Expect = 1e-06
 Identities = 29/34 (85%), Positives = 31/34 (91%)
 Frame = +1

Query: 523  RNVGTKRTLGEKLLTDMLQPAEGTGTSPEKKVRK 624
            RNVGTK+  G+KLLTDMLQPAE TGTSPEKKVRK
Sbjct: 1234 RNVGTKQLSGQKLLTDMLQPAESTGTSPEKKVRK 1267



 Score = 59.7 bits (143), Expect = 4e-06
 Identities = 29/37 (78%), Positives = 30/37 (81%)
 Frame = +2

Query: 788  KKVRKMRESPFNKKSGSILGRAANKDKRPSEDLSSGS 898
            KKVRKMRESPFNKKSGS+LGR ANKD  P  D S GS
Sbjct: 1263 KKVRKMRESPFNKKSGSVLGRDANKDISPLADFSEGS 1299


>ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago truncatula]
 gb|AES69522.2| DNA topoisomerase IV subunit A [Medicago truncatula]
          Length = 1504

 Score = 61.2 bits (147), Expect = 1e-06
 Identities = 29/34 (85%), Positives = 31/34 (91%)
 Frame = +1

Query: 523  RNVGTKRTLGEKLLTDMLQPAEGTGTSPEKKVRK 624
            RNVG+K TLG+KLLTDMLQPAE  GTSPEKKVRK
Sbjct: 1380 RNVGSKSTLGQKLLTDMLQPAESIGTSPEKKVRK 1413



 Score = 58.9 bits (141), Expect = 7e-06
 Identities = 29/37 (78%), Positives = 30/37 (81%)
 Frame = +2

Query: 788  KKVRKMRESPFNKKSGSILGRAANKDKRPSEDLSSGS 898
            KKVRKMRESPFNKKSGSILG+ AN D  P  DLS GS
Sbjct: 1409 KKVRKMRESPFNKKSGSILGKTANMDISPIADLSEGS 1445


>dbj|GAU18535.1| hypothetical protein TSUD_325240 [Trifolium subterraneum]
          Length = 1336

 Score = 58.9 bits (141), Expect = 6e-06
 Identities = 28/34 (82%), Positives = 30/34 (88%)
 Frame = +1

Query: 523  RNVGTKRTLGEKLLTDMLQPAEGTGTSPEKKVRK 624
            RNVGTK+TLG+KLLT MLQPAE T TSPEKK RK
Sbjct: 1232 RNVGTKQTLGKKLLTGMLQPAESTATSPEKKARK 1265