BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus22_contig00003152
         (1211 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceu...   719   0.0  
ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [C...   602   0.0  
sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase;...   598   0.0  
ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago ...   598   0.0  
ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [C...   571   0.0  
ref|XP_015959793.1| isoflavone 3'-hydroxylase [Arachis duranensis]    561   0.0  
gb|AAO31712.1| cytochrome P450 [Pisum sativum]                        556   0.0  
ref|XP_016197998.1| isoflavone 3'-hydroxylase [Arachis ipaensis]      556   0.0  
ref|XP_020224833.1| LOW QUALITY PROTEIN: isoflavone 3'-hydroxyla...   540   0.0  
gb|ABD17289.1| isoflavone 3'-hydroxylase, partial [Astragalus mo...   508   e-179
gb|AFC88269.1| isoflavone 3'-hydroxylase 1, partial [Astragalus ...   496   e-174
ref|XP_007158858.1| hypothetical protein PHAVU_002G187800g [Phas...   505   e-174
gb|KYP60203.1| Isoflavone 2'-hydroxylase [Cajanus cajan]              505   e-174
ref|XP_017405622.1| PREDICTED: isoflavone 3'-hydroxylase-like [V...   499   e-172
ref|XP_014506448.1| isoflavone 3'-hydroxylase [Vigna radiata var...   497   e-171
ref|XP_020223955.1| isoflavone 3'-hydroxylase-like [Cajanus cajan]    498   e-171
ref|XP_003532671.1| PREDICTED: isoflavone 3'-hydroxylase-like [G...   492   e-169
dbj|BAT21113.1| cytochrome P450 [synthetic construct]                 486   e-167
dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]                487   e-167
sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase;...   486   e-167

>gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]
 gb|ATY39977.1| Isoflavone 3'-hydroxylase [Astragalus membranaceus]
          Length = 505

 Score =  719 bits (1857), Expect = 0.0
 Identities = 364/403 (90%), Positives = 364/403 (90%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            QSRRLKNL           NLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI
Sbjct: 25   QSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 84

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
            AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF
Sbjct: 85   AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 144

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
            HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV
Sbjct: 145  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 204

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
            TDVKEAKQFRDIISEILSLLGANNKG              EKRCKRISKRADAFLQGLIE
Sbjct: 205  TDVKEAKQFRDIISEILSLLGANNKGDFLPLLRLFDLDHLEKRCKRISKRADAFLQGLIE 264

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE
Sbjct: 265  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 324

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALR              E
Sbjct: 325  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRLHPPAPLLLPHSASE 384

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE
Sbjct: 385  DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 427


>ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
          Length = 500

 Score =  602 bits (1551), Expect = 0.0
 Identities = 301/403 (74%), Positives = 335/403 (83%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            QSR+LKNL           NLHHLKHP+HRTFTTLSQKYGD++SLWFGSRLVVVV+SPS+
Sbjct: 25   QSRKLKNLPPGPPTIPIIGNLHHLKHPIHRTFTTLSQKYGDVLSLWFGSRLVVVVSSPSL 84

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
              ECFTKND++LANRP+FLTGKYIF NYTTLGSASYGDHWRNLRRITTIDVLSN+RLNSF
Sbjct: 85   VNECFTKNDIILANRPQFLTGKYIFNNYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSF 144

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
             GVR DET RLV+KL  + + EG   FT VELRHRLTEMTFN MMRMISGKRYYGDDGDV
Sbjct: 145  LGVRTDETNRLVQKLLKNTISEG---FTKVELRHRLTEMTFNGMMRMISGKRYYGDDGDV 201

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
            TDV+EAKQFRDIISEI+SLLGANNKG              EKRCKRI+KRADAFLQGLIE
Sbjct: 202  TDVEEAKQFRDIISEIMSLLGANNKGDFLPLLRLFDLDDLEKRCKRIAKRADAFLQGLIE 261

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            + Q+ N   NDGDTMIDHLLKLRE+QPEYYSDHMIKGLIQAMLLAGTDTSAV IEWVM+E
Sbjct: 262  EIQSGNQ--NDGDTMIDHLLKLRESQPEYYSDHMIKGLIQAMLLAGTDTSAVAIEWVMSE 319

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN+P+VL KAK+EIET IG+++L+EE DLS+LPYLQN+I+E LR              E
Sbjct: 320  LLNHPDVLNKAKEEIETQIGRDKLIEEHDLSKLPYLQNIINETLRLHPPAPLLLPHYSSE 379

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            D T+GGFNVPKDTIIL+N+WAIHRDP LW+DP SFKPERFE E
Sbjct: 380  DFTLGGFNVPKDTIILSNVWAIHRDPNLWSDPLSFKPERFEIE 422


>sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase; AltName: Full=Cytochrome
            P450 81E9
 gb|AAQ20041.1| isoflavone 3'-hydroxylase, partial [Medicago truncatula]
          Length = 501

 Score =  598 bits (1543), Expect = 0.0
 Identities = 302/403 (74%), Positives = 335/403 (83%), Gaps = 1/403 (0%)
 Frame = +1

Query: 4    SRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIA 183
            SRRLKNL           NLHHLKHPLHRTFTTLSQ YGDI SLWFGSRLVVVV+SPS+A
Sbjct: 24   SRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLA 83

Query: 184  QECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFH 363
             ECFTKND++LANRP+FLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSN+RLNSF 
Sbjct: 84   HECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSFL 143

Query: 364  GVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVT 543
            GVR+DET RL++KL  DVV EG F FT VELR RLTEMTFNAMMRMISGKRYYGDDGDV+
Sbjct: 144  GVRRDETNRLIQKLLKDVVSEG-FGFTKVELRPRLTEMTFNAMMRMISGKRYYGDDGDVS 202

Query: 544  DVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIEQ 723
            DV+EAKQFR+IISE++SLLGANNKG              EKRCKRI+KR++AFL+GLIE+
Sbjct: 203  DVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIAKRSNAFLEGLIEE 262

Query: 724  HQNAN-HSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            H+  N HS  DG TMIDHLLKL E+QPEYYSDH+IKGLIQ MLLAGTDTSAVTIEWVM+E
Sbjct: 263  HRRGNIHS--DGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSE 320

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN+PEVLKKAK+E++T IGK +LV+EQDLS+LPYLQN+ISE LR              E
Sbjct: 321  LLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSE 380

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            DCTIG FNVPKDTIILTN+W IHRDP+ W D  SFKPERFEKE
Sbjct: 381  DCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKE 423


>ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago truncatula]
 gb|KEH37344.1| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 503

 Score =  598 bits (1543), Expect = 0.0
 Identities = 302/403 (74%), Positives = 335/403 (83%), Gaps = 1/403 (0%)
 Frame = +1

Query: 4    SRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIA 183
            SRRLKNL           NLHHLKHPLHRTFTTLSQ YGDI SLWFGSRLVVVV+SPS+A
Sbjct: 26   SRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFGSRLVVVVSSPSLA 85

Query: 184  QECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFH 363
             ECFTKND++LANRP+FLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSN+RLNSF 
Sbjct: 86   HECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNNRLNSFL 145

Query: 364  GVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVT 543
            GVR+DET RL++KL  DVV EG F FT VELR RLTEMTFNAMMRMISGKRYYGDDGDV+
Sbjct: 146  GVRRDETNRLIQKLLKDVVSEG-FGFTKVELRPRLTEMTFNAMMRMISGKRYYGDDGDVS 204

Query: 544  DVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIEQ 723
            DV+EAKQFR+IISE++SLLGANNKG              EKRCKRI+KR++AFL+GLIE+
Sbjct: 205  DVEEAKQFREIISEMMSLLGANNKGDFLPLLRLFDLDNLEKRCKRIAKRSNAFLEGLIEE 264

Query: 724  HQNAN-HSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            H+  N HS  DG TMIDHLLKL E+QPEYYSDH+IKGLIQ MLLAGTDTSAVTIEWVM+E
Sbjct: 265  HRRGNIHS--DGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSE 322

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN+PEVLKKAK+E++T IGK +LV+EQDLS+LPYLQN+ISE LR              E
Sbjct: 323  LLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSE 382

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            DCTIG FNVPKDTIILTN+W IHRDP+ W D  SFKPERFEKE
Sbjct: 383  DCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKE 425


>ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
          Length = 500

 Score =  571 bits (1471), Expect = 0.0
 Identities = 289/401 (72%), Positives = 325/401 (81%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            QSR+LKNL           NLH LKHP+H TFTTLSQKYGD++SLWFGSRLVVVV+SPS+
Sbjct: 25   QSRKLKNLPPGPPTIPIIGNLHQLKHPMHHTFTTLSQKYGDVLSLWFGSRLVVVVSSPSL 84

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
             QECF KND+VLANRP+ L GKYIFYNYTTLGS+SYGDHWRNLRRIT IDVLSN+RLNSF
Sbjct: 85   VQECFNKNDIVLANRPQLLAGKYIFYNYTTLGSSSYGDHWRNLRRITAIDVLSNNRLNSF 144

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
             GVR DET RL++KL ND V +  F  T VELRHRLTEMTFN MM MISGKRYYGDD DV
Sbjct: 145  LGVRTDETNRLIQKLLNDTVSDQGF--TKVELRHRLTEMTFNGMMMMISGKRYYGDDRDV 202

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
            TDV+EAKQFRDIISEI+SLLGAN K                KR K+I+KRADAFLQGLIE
Sbjct: 203  TDVEEAKQFRDIISEIMSLLGAN-KSEFLPFLRLFDLDDLTKRYKKIAKRADAFLQGLIE 261

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            +HQ+ N S+  GDTMIDHLL LR++QPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVM+E
Sbjct: 262  EHQSGNQSN--GDTMIDHLLNLRKSQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMSE 319

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN+P+VL KAK+EIET IGK++L+EE DLS+LPYLQN+I+E LR              E
Sbjct: 320  LLNHPDVLNKAKEEIETQIGKDKLIEEHDLSKLPYLQNIINETLRLHPPAPLSLPHYSSE 379

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFE 1203
            D T+GGFNVPKDTIILTN+WAIHRDP+ W+D SSFKPERFE
Sbjct: 380  DFTLGGFNVPKDTIILTNVWAIHRDPKYWSDASSFKPERFE 420


>ref|XP_015959793.1| isoflavone 3'-hydroxylase [Arachis duranensis]
          Length = 504

 Score =  561 bits (1446), Expect = 0.0
 Identities = 283/403 (70%), Positives = 321/403 (79%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            QSRR KNL           NLH+LK PLHR F  L+  YGDI+SLWFG+RLVVVV+SPS+
Sbjct: 26   QSRRFKNLPPGPPTLPFLGNLHYLKPPLHRIFAGLAASYGDIMSLWFGNRLVVVVSSPSL 85

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
            A ECFTKNDVVLANRP+FLTGKYIFYNYTTLGSASYGDHWRNLRRITT+DVLS+HRLNSF
Sbjct: 86   AHECFTKNDVVLANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTVDVLSSHRLNSF 145

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
              VRKDET RL+EKL  D     N  F  VELR RLTEMTFN MMRMISGKRYYGDD ++
Sbjct: 146  LDVRKDETKRLIEKLWKDT---RNGEFVKVELRSRLTEMTFNGMMRMISGKRYYGDDVEM 202

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
            +DV+EAKQFR+II+EILSLLGANNKG              EKR K I+KRAD+FLQGLI+
Sbjct: 203  SDVEEAKQFREIITEILSLLGANNKGDFMPLAKFFDFDNLEKRLKNIAKRADSFLQGLIQ 262

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            +H+ A  S+ D DTMI HLLKL+ETQPEYYSD MIKGLIQAMLLAGTDTSAV +EWVMAE
Sbjct: 263  EHRVAAGSNADSDTMIFHLLKLQETQPEYYSDLMIKGLIQAMLLAGTDTSAVAMEWVMAE 322

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN+PE++KK K E++T IGK+RLVEEQDLS+LPYLQNVISE+LR              E
Sbjct: 323  LLNHPEIMKKVKDELDTQIGKDRLVEEQDLSKLPYLQNVISESLRLHPPAPLLLPHSASE 382

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            DCTIGG+N+PKDTI+LTN+W IHRDP +W D  SF+PERFEKE
Sbjct: 383  DCTIGGYNIPKDTIVLTNVWLIHRDPNIWADAESFRPERFEKE 425


>gb|AAO31712.1| cytochrome P450 [Pisum sativum]
          Length = 502

 Score =  556 bits (1433), Expect = 0.0
 Identities = 281/404 (69%), Positives = 321/404 (79%), Gaps = 1/404 (0%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            QSRRLKNL           NLHHLKHPLHRTF TLSQKYGDI+SLWFGSRLVVV++S S+
Sbjct: 25   QSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFATLSQKYGDIVSLWFGSRLVVVISSASL 84

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
             +ECF KNDVVLANRP+FLTGKYIFY+YTTLGS SYGDHWRNLRRITTIDVLSN+RL SF
Sbjct: 85   VEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNLRRITTIDVLSNNRLTSF 144

Query: 361  HGVRKDETLRLVEKLRNDVVKEG-NFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGD 537
             GVR DE  RL++    +++K G +  F  VELR  LTEMTFN MMRMISGKRYYGDDGD
Sbjct: 145  LGVRTDEANRLIQ----NIIKHGASRDFIKVELRSILTEMTFNGMMRMISGKRYYGDDGD 200

Query: 538  VTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLI 717
            VT+V+EAKQFR+IISEILSLLGANNKG              EKRCK+I+ RADAFL+GLI
Sbjct: 201  VTEVEEAKQFREIISEILSLLGANNKGDFLPLLRWFDLDDLEKRCKKIANRADAFLEGLI 260

Query: 718  EQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMA 897
            ++H++ANH +  GDTMIDHLLKL E Q      H+IKGLIQAMLLAGTDTSA+ IEWVM+
Sbjct: 261  QEHRSANHGN--GDTMIDHLLKLSEVQLNIIPSHIIKGLIQAMLLAGTDTSALAIEWVMS 318

Query: 898  ELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXX 1077
            ELLN+PEVL KAK+EIET +GK ++++EQDL +LPYLQN+ISEAL+              
Sbjct: 319  ELLNHPEVLNKAKEEIETQVGKNKIIDEQDLPKLPYLQNIISEALKLHPPAPLLLPHYSS 378

Query: 1078 EDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            EDCTIGGFNVPKDTIILTN+WAIHRDP  W+D  SFKPERFEKE
Sbjct: 379  EDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKE 422


>ref|XP_016197998.1| isoflavone 3'-hydroxylase [Arachis ipaensis]
          Length = 529

 Score =  556 bits (1432), Expect = 0.0
 Identities = 280/403 (69%), Positives = 318/403 (78%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            QSRR KNL           NLH+LK P+HR F  L+  YGDI+SLWFG+RLVVVV+SPS+
Sbjct: 51   QSRRFKNLPPGPPTLPFLGNLHYLKPPVHRIFAGLAASYGDIMSLWFGNRLVVVVSSPSL 110

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
            A ECFTKNDVVLANRP+FLTGKYIFYNYTTLGSASYGDHWRNLRRITT+DVLS+HRLNSF
Sbjct: 111  AHECFTKNDVVLANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITTVDVLSSHRLNSF 170

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
              VRKDET RL+EKL  D     N  F  VELR RLTEMTFN MMRMISGKRYYGDD ++
Sbjct: 171  LDVRKDETKRLIEKLWKDT---RNGEFVKVELRSRLTEMTFNGMMRMISGKRYYGDDVEM 227

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
             DV+EAKQFR+II+EILSLLGA+NKG              EKR K I+KRAD+FLQGLI+
Sbjct: 228  NDVEEAKQFREIITEILSLLGASNKGDFMPLAKFFDFDHLEKRLKNIAKRADSFLQGLIQ 287

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            +H+ A  S+ D DTMI HLLKL+ETQPEYYSD MIKGLIQAMLLAGTDTSAV +EW MAE
Sbjct: 288  EHRVAAGSNADSDTMIFHLLKLQETQPEYYSDLMIKGLIQAMLLAGTDTSAVAMEWAMAE 347

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN+PE++KK K E++T IGK+RLVEEQDLS+LPYLQNVISE LR              E
Sbjct: 348  LLNHPEIMKKVKDELDTQIGKDRLVEEQDLSKLPYLQNVISETLRLHPPAPLLLPHSASE 407

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            DCTIGG+N+PKDTI+LTN+W IHRDP +W D  SF+PERFEKE
Sbjct: 408  DCTIGGYNIPKDTIVLTNVWLIHRDPNIWADAESFRPERFEKE 450


>ref|XP_020224833.1| LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cajanus cajan]
          Length = 500

 Score =  540 bits (1390), Expect = 0.0
 Identities = 276/403 (68%), Positives = 311/403 (77%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            QSRR KNL           NLH LK P+HR+ + L + YGDI+SLWFGSRLVVVV+SPS+
Sbjct: 25   QSRRPKNLPPGPPPFPLIGNLHRLKPPIHRSISALCRTYGDIVSLWFGSRLVVVVSSPSL 84

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
             QECF +NDVVLANRP+FL GKYIFYN     SASYGDHWRNLRRITTIDVLS HRLNSF
Sbjct: 85   VQECFGRNDVVLANRPRFLAGKYIFYNXR---SASYGDHWRNLRRITTIDVLSTHRLNSF 141

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
             G+R+DE  RLV +L  D       +   V LR RLTEMTFNAMMRMISGKRYYGDD DV
Sbjct: 142  VGIRRDEARRLVARLGQDATCREGEALKKVALRPRLTEMTFNAMMRMISGKRYYGDDCDV 201

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
            TD +EAKQFR+IISE+LSLLGANNKG              EKR KRI+KRADAFLQ LIE
Sbjct: 202  TDAEEAKQFREIISEMLSLLGANNKGDFLPLLRLFDFDDLEKRLKRIAKRADAFLQSLIE 261

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            +H++  H +   DTMIDHLLKL E+QPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVM+E
Sbjct: 262  EHRSGKHGN--ADTMIDHLLKLGESQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMSE 319

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLNNPEVLKKAK EI+T IGK+RLVEEQDLS+LPYLQN+ISE LR              E
Sbjct: 320  LLNNPEVLKKAKDEIDTQIGKDRLVEEQDLSKLPYLQNIISETLRLHPPAPLLLPHESSE 379

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            DCTIGG+++P++TI+LTN W IH DP+LW+DP SF PERFEKE
Sbjct: 380  DCTIGGYHIPRNTIVLTNAWLIHSDPKLWSDPMSFMPERFEKE 422


>gb|ABD17289.1| isoflavone 3'-hydroxylase, partial [Astragalus mongholicus]
          Length = 268

 Score =  508 bits (1308), Expect = e-179
 Identities = 253/267 (94%), Positives = 253/267 (94%)
 Frame = +1

Query: 58  NLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFL 237
           NLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFL
Sbjct: 2   NLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFL 61

Query: 238 TGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDV 417
           TGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDV
Sbjct: 62  TGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDV 121

Query: 418 VKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSL 597
           VKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSL
Sbjct: 122 VKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSL 181

Query: 598 LGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHL 777
           LGANNKG              EKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHL
Sbjct: 182 LGANNKGDFLPLLRLFDLDHLEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHL 241

Query: 778 LKLRETQPEYYSDHMIKGLIQAMLLAG 858
           LKLRETQPEYYSDHMIKGLIQAMLLAG
Sbjct: 242 LKLRETQPEYYSDHMIKGLIQAMLLAG 268


>gb|AFC88269.1| isoflavone 3'-hydroxylase 1, partial [Astragalus membranaceus]
          Length = 262

 Score =  496 bits (1277), Expect = e-174
 Identities = 247/262 (94%), Positives = 247/262 (94%)
 Frame = +1

Query: 64  HHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTG 243
           HHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTG
Sbjct: 1   HHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTG 60

Query: 244 KYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVK 423
           KYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVK
Sbjct: 61  KYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVK 120

Query: 424 EGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLG 603
           EGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFR IISEILSLLG
Sbjct: 121 EGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRGIISEILSLLG 180

Query: 604 ANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLK 783
           ANNKG              EKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLK
Sbjct: 181 ANNKGDFLPLLRLFDLDHLEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLK 240

Query: 784 LRETQPEYYSDHMIKGLIQAML 849
           LRETQPEYYSDHMIKGLIQAML
Sbjct: 241 LRETQPEYYSDHMIKGLIQAML 262


>ref|XP_007158858.1| hypothetical protein PHAVU_002G187800g [Phaseolus vulgaris]
 gb|ESW30852.1| hypothetical protein PHAVU_002G187800g [Phaseolus vulgaris]
          Length = 500

 Score =  505 bits (1300), Expect = e-174
 Identities = 249/403 (61%), Positives = 301/403 (74%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            Q RRLKNL           NLH +K PLHR+F +LSQKYG I SLWFGSR VVV+ SP++
Sbjct: 23   QRRRLKNLPPHPPTLPIIGNLHLIKPPLHRSFLSLSQKYGPIFSLWFGSRYVVVITSPTL 82

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
             QECFTK D VLANRP+ LTGKY+FYNYT++GS+ YG+HWRNLRRI TIDVLS  RLNSF
Sbjct: 83   VQECFTKYDTVLANRPRLLTGKYLFYNYTSMGSSPYGEHWRNLRRIITIDVLSTQRLNSF 142

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
              +R++ET+R+++KL  +  K     FT V++R RLTEMTFN MMRMISGKRYYGDDGDV
Sbjct: 143  FEIRREETMRVIQKLVRETCK----GFTHVQIRPRLTEMTFNNMMRMISGKRYYGDDGDV 198

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
            TD +EAKQFRDIISE++SLLGANNKG              EKR K IS+RADAFLQGL+E
Sbjct: 199  TDAEEAKQFRDIISEVMSLLGANNKGDFLPLLRWFDFDGLEKRLKSISRRADAFLQGLLE 258

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            +H++  H  N   TMI+HLL ++E+QPEYYSDH+IKGLIQ MLLAGTDT+AVT+EW +A 
Sbjct: 259  EHRSGKHKAN---TMIEHLLTMQESQPEYYSDHIIKGLIQGMLLAGTDTTAVTMEWAVAS 315

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN PE+LKKA+ EI+  +G++RLVEE D+  LPYLQN+I E LR              +
Sbjct: 316  LLNEPEILKKAQDEIKKCVGEDRLVEESDIPNLPYLQNIIYETLRLFSPAPLLLPHYSSD 375

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
             CTIGGF +P  TI+L N WAI RDPE W+D + FKPERFE+E
Sbjct: 376  HCTIGGFTIPPHTIVLINAWAIQRDPETWSDATCFKPERFEQE 418


>gb|KYP60203.1| Isoflavone 2'-hydroxylase [Cajanus cajan]
          Length = 506

 Score =  505 bits (1300), Expect = e-174
 Identities = 267/421 (63%), Positives = 302/421 (71%), Gaps = 18/421 (4%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            QSRR KNL           NLH LK P+HR+ + L + YGDI+SLWFGSRLVVVV+SPS+
Sbjct: 25   QSRRPKNLPPGPPPFPLIGNLHRLKPPIHRSISALCRTYGDIVSLWFGSRLVVVVSSPSL 84

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
             QECF +NDVVLANRP+               SASYGDHWRNLRRITTIDVLS HRLNSF
Sbjct: 85   VQECFGRNDVVLANRPR---------------SASYGDHWRNLRRITTIDVLSTHRLNSF 129

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
             G+R+DE  RLV +L  D       +   V LR RLTEMTFNAMMRMISGKRYYGDD DV
Sbjct: 130  VGIRRDEARRLVARLGQDATCREGEALKKVALRPRLTEMTFNAMMRMISGKRYYGDDCDV 189

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
            TD +EAKQFR+IISE+LSLLGANNKG              EKR KRI+KRADAFLQ LIE
Sbjct: 190  TDAEEAKQFREIISEMLSLLGANNKGDFLPLLRLFDFDDLEKRLKRIAKRADAFLQSLIE 249

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLI------------------QAM 846
            +H++  H   + DTMIDHLLKL E+QPEYYSDHMIKGLI                  QAM
Sbjct: 250  EHRSGKH--GNADTMIDHLLKLGESQPEYYSDHMIKGLIQSVFPELSFGLHYECAFRQAM 307

Query: 847  LLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISE 1026
            LLAGTDTSAVTIEWVM+ELLNNPEVLKKAK EI+T IGK+RLVEEQDLS+LPYLQN+ISE
Sbjct: 308  LLAGTDTSAVTIEWVMSELLNNPEVLKKAKDEIDTQIGKDRLVEEQDLSKLPYLQNIISE 367

Query: 1027 ALRXXXXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEK 1206
             LR              EDCTIGG+++P++TI+LTN W IH DP+LW+DP SF PERFEK
Sbjct: 368  TLRLHPPAPLLLPHESSEDCTIGGYHIPRNTIVLTNAWLIHSDPKLWSDPMSFMPERFEK 427

Query: 1207 E 1209
            E
Sbjct: 428  E 428


>ref|XP_017405622.1| PREDICTED: isoflavone 3'-hydroxylase-like [Vigna angularis]
 gb|KOM25512.1| hypothetical protein LR48_Vigan107s003100 [Vigna angularis]
 dbj|BAT74197.1| hypothetical protein VIGAN_01181600 [Vigna angularis var. angularis]
          Length = 500

 Score =  499 bits (1286), Expect = e-172
 Identities = 247/401 (61%), Positives = 301/401 (75%)
 Frame = +1

Query: 7    RRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQ 186
            RRL+NL           NLH +K PLHR+  +LSQ+YG I SLWFGSR VVV+ SP++ Q
Sbjct: 25   RRLENLPPHPPTLPIIGNLHLIKPPLHRSLLSLSQQYGPIFSLWFGSRFVVVITSPTLVQ 84

Query: 187  ECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHG 366
            ECFTK D VLANRP+FLTGKY+FYNYT++GS+ YGDHWRNLRRI TIDVLS  RLNSF  
Sbjct: 85   ECFTKYDTVLANRPRFLTGKYLFYNYTSMGSSPYGDHWRNLRRIITIDVLSTQRLNSFFE 144

Query: 367  VRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTD 546
            VR++ET+R+V+KL    V+E +  F  V++R RLTEMTFN MMRMISGKRYYGDDGDVTD
Sbjct: 145  VRREETMRVVQKL----VRETSKGFALVQIRPRLTEMTFNNMMRMISGKRYYGDDGDVTD 200

Query: 547  VKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIEQH 726
             +EAKQFR II+E++SLLGANNKG              EKR K IS RADAFLQGL+E+H
Sbjct: 201  AEEAKQFRAIITEVMSLLGANNKGDFLPLLRWFDFDGLEKRLKSISTRADAFLQGLLEEH 260

Query: 727  QNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAELL 906
            ++  H  N   TMI+HLL ++ETQPEYYSDH+IKGLIQ MLLAGTDT+AVT+EW MA LL
Sbjct: 261  RSGKHKTN---TMIEHLLTMQETQPEYYSDHIIKGLIQGMLLAGTDTTAVTMEWAMASLL 317

Query: 907  NNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXEDC 1086
            N+PE+LKKA+ EI+  +G++RLV+E D+  LPY+QN+I E LR              E C
Sbjct: 318  NHPEILKKAQDEIDKCVGQDRLVDESDIPNLPYIQNIIYETLRLFSPAPMLLPHYSSEQC 377

Query: 1087 TIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            T+GGF VP+DTI+L N WAI RDP+ W+D + FKPERF +E
Sbjct: 378  TLGGFTVPRDTIVLINAWAIQRDPQTWSDATCFKPERFGQE 418


>ref|XP_014506448.1| isoflavone 3'-hydroxylase [Vigna radiata var. radiata]
          Length = 500

 Score =  497 bits (1279), Expect = e-171
 Identities = 246/401 (61%), Positives = 300/401 (74%)
 Frame = +1

Query: 7    RRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQ 186
            RRL+NL           NLH +K PLHR+  +LSQ+YG I SLWFGSR VVV+ SP++ Q
Sbjct: 25   RRLENLPPHPPTLPIIGNLHLIKPPLHRSLLSLSQQYGPIFSLWFGSRFVVVITSPTLVQ 84

Query: 187  ECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHG 366
            ECFTK D+VLANRP+FLTGKY+FYNYT++GS+SYGDHWRNLRRI TIDVLS  RLNSF  
Sbjct: 85   ECFTKYDIVLANRPRFLTGKYLFYNYTSMGSSSYGDHWRNLRRIITIDVLSTQRLNSFFE 144

Query: 367  VRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTD 546
            VR++ET+R+++KL  +  K     F  V++R RLTEMTFN MMRMISGKRYYGDDGDVTD
Sbjct: 145  VRREETMRVMQKLVRETCK----GFALVQIRPRLTEMTFNNMMRMISGKRYYGDDGDVTD 200

Query: 547  VKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIEQH 726
             +EAKQFR II+E++SLLGANNKG              EKR K IS RADAFLQGL+E+H
Sbjct: 201  AEEAKQFRAIITEVMSLLGANNKGDFLPLLRWFDFDGLEKRLKSISTRADAFLQGLLEEH 260

Query: 727  QNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAELL 906
            ++  H  N   TMI+HLL ++ETQPEYYSDH+IKGLIQ MLLAGTDT+AVT+EW MA LL
Sbjct: 261  RSGKHKTN---TMIEHLLTMQETQPEYYSDHIIKGLIQGMLLAGTDTTAVTMEWAMASLL 317

Query: 907  NNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXEDC 1086
            N+PE+LKKA+ EI+  +G++RLV+E D+  LPYLQN+I E LR              E  
Sbjct: 318  NHPEILKKAQDEIDNCVGEDRLVDESDIPNLPYLQNIIYETLRLFSPAPMLLPHYSSEQF 377

Query: 1087 TIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            T+GGF VP+DTI+L N WAI RDP+ W+D + FKPERF +E
Sbjct: 378  TLGGFTVPRDTIVLINAWAIQRDPQTWSDATCFKPERFGQE 418


>ref|XP_020223955.1| isoflavone 3'-hydroxylase-like [Cajanus cajan]
          Length = 537

 Score =  498 bits (1282), Expect = e-171
 Identities = 253/403 (62%), Positives = 294/403 (72%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            QSRR +NL           NLHHLK P H  F  LSQ YGD+ISL FG RLVVV++S S+
Sbjct: 55   QSRRFRNLPPGPPTLPIIGNLHHLKPPNHHAFAVLSQTYGDVISLRFGRRLVVVLSSLSV 114

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
             Q+CF  NDVVLANRP+FL GKY+ Y+YTTLGS SYG HWRNLRRI T+DVLS HRLNSF
Sbjct: 115  VQQCFAANDVVLANRPRFLVGKYVSYDYTTLGSTSYGHHWRNLRRIATVDVLSTHRLNSF 174

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
             GVR+DE  RLV KL  D        F  VELR RLTEMTFN MMRMI+G+R YG D DV
Sbjct: 175  VGVRRDEIRRLVMKLGQDATCREGEGFAKVELRWRLTEMTFNNMMRMITGRRCYGQDCDV 234

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
             + + A+QFR++I+E++SL GANNKG              EKR KRI++RADAFL  LIE
Sbjct: 235  KEAETARQFREVITEMVSLSGANNKGDFLPLLRLFDFEDLEKRLKRIAERADAFLHSLIE 294

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            +H++  H     D MIDHLLKL + QPEYYSDH+IKGLIQAMLLAGTDT A+TIEWVM+E
Sbjct: 295  EHRSGKH--GSVDNMIDHLLKLCQLQPEYYSDHIIKGLIQAMLLAGTDTPALTIEWVMSE 352

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLNNPEVLKKAK EI T IGK+RLVEEQDL +L YLQN+ISE  R              E
Sbjct: 353  LLNNPEVLKKAKDEINTHIGKDRLVEEQDLPKLSYLQNIISETFRLHPLAPLLLPHESSE 412

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            DCTIGG+++P+ TI+LTN W IHRDP LW+DP+SFKPERFE+E
Sbjct: 413  DCTIGGYHIPRGTIVLTNAWLIHRDPILWSDPTSFKPERFERE 455


>ref|XP_003532671.1| PREDICTED: isoflavone 3'-hydroxylase-like [Glycine max]
 gb|KHN28918.1| Isoflavone 2'-hydroxylase [Glycine soja]
 gb|KRH42430.1| hypothetical protein GLYMA_08G089400 [Glycine max]
          Length = 500

 Score =  492 bits (1267), Expect = e-169
 Identities = 239/403 (59%), Positives = 301/403 (74%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            Q RR+KNL           NLH++K PLHR+  +LS+KYG I SLWFGSR VVV++SP++
Sbjct: 20   QRRRVKNLPPCPPSLPLIGNLHYIKSPLHRSLLSLSEKYGPIFSLWFGSRFVVVISSPTL 79

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
             QECFTK+D+VLANRP+FLTGKY+FYNY+++GS+ YGDHWRNLRRI TIDVLS  RLNSF
Sbjct: 80   LQECFTKHDIVLANRPRFLTGKYLFYNYSSMGSSPYGDHWRNLRRIITIDVLSTSRLNSF 139

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
              +R++ET+R+++KL  +        F  V LR RLTEMTFN MMRMISGKRYYGDD + 
Sbjct: 140  FEIRREETMRVIQKLARETCN----GFALVHLRPRLTEMTFNNMMRMISGKRYYGDDIEA 195

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
             D +EAKQFRDI++E++SLLGANNKG              EKR K IS RAD+FLQGL+E
Sbjct: 196  ADAEEAKQFRDIMTEVMSLLGANNKGDFLPFLRWFDFDGLEKRLKVISTRADSFLQGLLE 255

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            +H++  H  N   TMI+HLL ++E+QP YYSDH+IKGLIQ MLLAGTDT+AV IEW ++ 
Sbjct: 256  EHRSGKHKAN---TMIEHLLTMQESQPHYYSDHIIKGLIQGMLLAGTDTTAVAIEWAVSS 312

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN+PE+LKKAK EI+  +G++RLV+E D+ +LPYLQN+I E LR              E
Sbjct: 313  LLNHPEILKKAKDEIDNMVGQDRLVDESDIPKLPYLQNIIYETLRLFAPAPLLLPHYSSE 372

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            +CTIGGF +P+DTI+L N WAI RDPE W+D + FKPERFE+E
Sbjct: 373  ECTIGGFTIPRDTIVLINAWAIQRDPEHWSDATCFKPERFEQE 415


>dbj|BAT21113.1| cytochrome P450 [synthetic construct]
          Length = 477

 Score =  486 bits (1252), Expect = e-167
 Identities = 240/403 (59%), Positives = 301/403 (74%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            ++R+ KNL           NLHHLK PLHRTF  LS+KYG + SLWFGSRLVVVV+S S 
Sbjct: 3    RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASE 62

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
             Q+CFTKNDVVLANRP+FL+GKYIFYNYTTLGS SYG+HWRNLRRIT +DVLSNHR+NSF
Sbjct: 63   FQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSF 122

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
             G+R+DET RL+ +L +D     + +F ++EL  RL +MTFN +MRMISGKRYYG+D D 
Sbjct: 123  SGIRRDETQRLITRLADD----SSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDT 178

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
            +D++EA QFRD++SE+L L GANNK               EKR K IS + DAFL+GLIE
Sbjct: 179  SDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIE 238

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            +H+      N   TMIDHLL L+++QPEYY+D +IKGL  AMLLAGTD+SAVT+EW M+ 
Sbjct: 239  EHRTKKERAN---TMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSN 295

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN+PEVLKK K E++T +G++RLV+E DL +L YL+NVI+E LR              +
Sbjct: 296  LLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSD 355

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            +C IGG+ VP+DTI+L N WAIHRDPELWT+ ++FKPERFEK+
Sbjct: 356  ECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKK 398


>dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  487 bits (1253), Expect = e-167
 Identities = 239/403 (59%), Positives = 303/403 (75%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            ++R+ KNL           NLHHLK PLHRTF  LS+KYG +ISLWFGSRLVVVV+S S 
Sbjct: 25   RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVISLWFGSRLVVVVSSASE 84

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
             Q+CFTKNDVVLANRP+FL+GKYIFYNYTTLGS SYG+HWRNLRRIT +DVLSNHR+NSF
Sbjct: 85   FQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSF 144

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
             G+R+DET RL+ +L +D     + +F ++EL +R  +MTFN +MRMISGKRYYG+D D+
Sbjct: 145  SGIRRDETQRLITRLADD----SSTNFAEIELSYRFYDMTFNNIMRMISGKRYYGEDCDM 200

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
            +D++EA QFRD++SE+L L GANNK               EKR K IS + DAFL+GLI+
Sbjct: 201  SDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIQ 260

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            +H+      N   TMIDHLL L+++QPEYY+D +IKGL  AMLLAGTD+SAVT+EW M+ 
Sbjct: 261  EHRAKKERAN---TMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSN 317

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN+PEVLKK K E++T +G++RLV+E DL +L YL+NVI+E LR              +
Sbjct: 318  LLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLSYLKNVINETLRLYTPAPLLLPHSTSD 377

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            +C IGG+ VP+DTI+L N WAIHRDPELWT+ ++FKPERFEK+
Sbjct: 378  ECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKK 420


>sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase; AltName: Full=CYP GE-3;
            AltName: Full=Cytochrome P450 81E1; AltName:
            Full=Cytochrome P450 91A4
 dbj|BAA22422.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  486 bits (1252), Expect = e-167
 Identities = 240/403 (59%), Positives = 301/403 (74%)
 Frame = +1

Query: 1    QSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSI 180
            ++R+ KNL           NLHHLK PLHRTF  LS+KYG + SLWFGSRLVVVV+S S 
Sbjct: 25   RARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSASE 84

Query: 181  AQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSF 360
             Q+CFTKNDVVLANRP+FL+GKYIFYNYTTLGS SYG+HWRNLRRIT +DVLSNHR+NSF
Sbjct: 85   FQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINSF 144

Query: 361  HGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDV 540
             G+R+DET RL+ +L +D     + +F ++EL  RL +MTFN +MRMISGKRYYG+D D 
Sbjct: 145  SGIRRDETQRLITRLADD----SSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCDT 200

Query: 541  TDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIE 720
            +D++EA QFRD++SE+L L GANNK               EKR K IS + DAFL+GLIE
Sbjct: 201  SDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLIE 260

Query: 721  QHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAE 900
            +H+      N   TMIDHLL L+++QPEYY+D +IKGL  AMLLAGTD+SAVT+EW M+ 
Sbjct: 261  EHRTKKERAN---TMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMSN 317

Query: 901  LLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXE 1080
            LLN+PEVLKK K E++T +G++RLV+E DL +L YL+NVI+E LR              +
Sbjct: 318  LLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTSD 377

Query: 1081 DCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 1209
            +C IGG+ VP+DTI+L N WAIHRDPELWT+ ++FKPERFEK+
Sbjct: 378  ECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKK 420