BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Angelica27_contig00013732
         (1362 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_017216773.1 PREDICTED: E4 SUMO-protein ligase PIAL2-like isof...   631   0.0  
XP_017216772.1 PREDICTED: E4 SUMO-protein ligase PIAL2-like isof...   626   0.0  
KZM88141.1 hypothetical protein DCAR_025216 [Daucus carota subsp...   499   e-168
XP_019081226.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X...   201   7e-54
CBI40413.3 unnamed protein product, partial [Vitis vinifera]          201   1e-53
XP_019081225.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X...   201   2e-53
XP_019081224.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X...   201   2e-53
XP_010661811.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X...   201   2e-53
XP_018841222.1 PREDICTED: E4 SUMO-protein ligase PIAL1-like isof...   182   2e-46
XP_018841221.1 PREDICTED: E4 SUMO-protein ligase PIAL1-like isof...   182   2e-46
XP_018841220.1 PREDICTED: E4 SUMO-protein ligase PIAL1-like isof...   182   2e-46
KDP25375.1 hypothetical protein JCGZ_20531 [Jatropha curcas]          180   3e-46
XP_012086815.1 PREDICTED: uncharacterized protein LOC105645744 i...   180   8e-46
XP_012086814.1 PREDICTED: uncharacterized protein LOC105645744 i...   180   8e-46
XP_010661825.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X...   176   2e-44
XP_010661824.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X...   176   2e-44
XP_019052072.1 PREDICTED: E4 SUMO-protein ligase PIAL1-like isof...   176   2e-44
XP_010247436.1 PREDICTED: E4 SUMO-protein ligase PIAL1-like isof...   176   3e-44
CDP12081.1 unnamed protein product [Coffea canephora]                 174   1e-43
CDP08753.1 unnamed protein product [Coffea canephora]                 171   3e-43

>XP_017216773.1 PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X2 [Daucus
            carota subsp. sativus]
          Length = 691

 Score =  631 bits (1627), Expect = 0.0
 Identities = 319/446 (71%), Positives = 362/446 (81%), Gaps = 6/446 (1%)
 Frame = -3

Query: 1360 AIKISNGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPGIEMALQTSLNCPLSKSRMR 1181
            AI   NGPYII I VMCVIPTSGTPQLQDYVQPVVASG   +EMALQTSLNCP+SK+RMR
Sbjct: 247  AIGNFNGPYIIAIAVMCVIPTSGTPQLQDYVQPVVASGDTDMEMALQTSLNCPISKNRMR 306

Query: 1180 TPVKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENT 1001
            TP+KGHLCKH QCFDYDSYLSINSR+P WRCPLCDEPVSC DIRIDQN+ KVLREV E+T
Sbjct: 307  TPIKGHLCKHMQCFDYDSYLSINSRRPCWRCPLCDEPVSCIDIRIDQNIAKVLREVEEDT 366

Query: 1000 VDVLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSDPSRRNQSEADNCSQQISPTK 821
              VLVSKDGSWKVVSE IDP   SASKTP F+EEKQN+S+P  +N S  +N SQQISPT 
Sbjct: 367  DHVLVSKDGSWKVVSEIIDPSFGSASKTPEFREEKQNQSNPIGKNLSRDENYSQQISPTT 426

Query: 820  NIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVKPNGLSAAMSTINDCKGCQL 641
            N++PSRV SMHQNT +S+SLAA+CTNLNDQG  N R      NG+S AMS I DC+GC  
Sbjct: 427  NVEPSRV-SMHQNTSNSSSLAANCTNLNDQGKKNSRVTGTNSNGMSIAMSPIIDCRGCHS 485

Query: 640  IQKDADLRNQTSGACINTSSSAN------SLSPLQNLVSQNSNAYILPTVEKNGCYQNSQ 479
            +QKD +LR Q+S +CINTSSSAN      S+S + +L  QNSNAYILPTVE+NG  QNS+
Sbjct: 486  LQKDTELRKQSSWSCINTSSSANPLSVQGSVSQVNSLPVQNSNAYILPTVEQNGYCQNSR 545

Query: 478  GSYTYQNLNKSSNNICEAVRVQQVQLQRDGKVQASLAAQDRQPSVFVPCTRQPRPEGERK 299
             ++TYQN   SSNN+CEAV VQQV  QRD ++QASL   +RQPSV   C++Q RPE E+K
Sbjct: 546  DTHTYQNSKNSSNNVCEAVHVQQVHSQRDDEIQASLNTHNRQPSVLDTCSKQSRPESEQK 605

Query: 298  QNMHEKKNQISHNDGLVELPSEGWRPSGRMRGSLQGQAYSEAYRQFIKQPTQHVRASELP 119
            QNMH +KNQ+SH  GLVELPSEGWRPSGRMRGSLQGQAY+EAYR+FIKQPTQ V ASELP
Sbjct: 606  QNMHREKNQVSHEGGLVELPSEGWRPSGRMRGSLQGQAYAEAYRRFIKQPTQPVGASELP 665

Query: 118  PRLVATLSVAKSQSPNIKASSQTKTQ 41
             RL+ATLSVAKSQ PNIKAS+Q   Q
Sbjct: 666  SRLMATLSVAKSQRPNIKASAQATNQ 691


>XP_017216772.1 PREDICTED: E4 SUMO-protein ligase PIAL2-like isoform X1 [Daucus
            carota subsp. sativus]
          Length = 694

 Score =  626 bits (1614), Expect = 0.0
 Identities = 319/449 (71%), Positives = 362/449 (80%), Gaps = 9/449 (2%)
 Frame = -3

Query: 1360 AIKISNGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPG---IEMALQTSLNCPLSKS 1190
            AI   NGPYII I VMCVIPTSGTPQLQDYVQPVVASG      +EMALQTSLNCP+SK+
Sbjct: 247  AIGNFNGPYIIAIAVMCVIPTSGTPQLQDYVQPVVASGDTASTDMEMALQTSLNCPISKN 306

Query: 1189 RMRTPVKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVG 1010
            RMRTP+KGHLCKH QCFDYDSYLSINSR+P WRCPLCDEPVSC DIRIDQN+ KVLREV 
Sbjct: 307  RMRTPIKGHLCKHMQCFDYDSYLSINSRRPCWRCPLCDEPVSCIDIRIDQNIAKVLREVE 366

Query: 1009 ENTVDVLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSDPSRRNQSEADNCSQQIS 830
            E+T  VLVSKDGSWKVVSE IDP   SASKTP F+EEKQN+S+P  +N S  +N SQQIS
Sbjct: 367  EDTDHVLVSKDGSWKVVSEIIDPSFGSASKTPEFREEKQNQSNPIGKNLSRDENYSQQIS 426

Query: 829  PTKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVKPNGLSAAMSTINDCKG 650
            PT N++PSRV SMHQNT +S+SLAA+CTNLNDQG  N R      NG+S AMS I DC+G
Sbjct: 427  PTTNVEPSRV-SMHQNTSNSSSLAANCTNLNDQGKKNSRVTGTNSNGMSIAMSPIIDCRG 485

Query: 649  CQLIQKDADLRNQTSGACINTSSSAN------SLSPLQNLVSQNSNAYILPTVEKNGCYQ 488
            C  +QKD +LR Q+S +CINTSSSAN      S+S + +L  QNSNAYILPTVE+NG  Q
Sbjct: 486  CHSLQKDTELRKQSSWSCINTSSSANPLSVQGSVSQVNSLPVQNSNAYILPTVEQNGYCQ 545

Query: 487  NSQGSYTYQNLNKSSNNICEAVRVQQVQLQRDGKVQASLAAQDRQPSVFVPCTRQPRPEG 308
            NS+ ++TYQN   SSNN+CEAV VQQV  QRD ++QASL   +RQPSV   C++Q RPE 
Sbjct: 546  NSRDTHTYQNSKNSSNNVCEAVHVQQVHSQRDDEIQASLNTHNRQPSVLDTCSKQSRPES 605

Query: 307  ERKQNMHEKKNQISHNDGLVELPSEGWRPSGRMRGSLQGQAYSEAYRQFIKQPTQHVRAS 128
            E+KQNMH +KNQ+SH  GLVELPSEGWRPSGRMRGSLQGQAY+EAYR+FIKQPTQ V AS
Sbjct: 606  EQKQNMHREKNQVSHEGGLVELPSEGWRPSGRMRGSLQGQAYAEAYRRFIKQPTQPVGAS 665

Query: 127  ELPPRLVATLSVAKSQSPNIKASSQTKTQ 41
            ELP RL+ATLSVAKSQ PNIKAS+Q   Q
Sbjct: 666  ELPSRLMATLSVAKSQRPNIKASAQATNQ 694


>KZM88141.1 hypothetical protein DCAR_025216 [Daucus carota subsp. sativus]
          Length = 721

 Score =  499 bits (1285), Expect = e-168
 Identities = 272/444 (61%), Positives = 313/444 (70%), Gaps = 12/444 (2%)
 Frame = -3

Query: 1360 AIKISNGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPG---IEMALQTSLNCPLSKS 1190
            AI   NGPYII I VMCVIPTSGTPQLQDYVQPVVASG      +EMALQTSLNCP+SK+
Sbjct: 259  AIGNFNGPYIIAIAVMCVIPTSGTPQLQDYVQPVVASGDTASTDMEMALQTSLNCPISKN 318

Query: 1189 RMRTPVKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVG 1010
            RMRTP+KGHLCKH Q                                       VLREV 
Sbjct: 319  RMRTPIKGHLCKHMQ---------------------------------------VLREVE 339

Query: 1009 ENTVDVLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSDPSRRNQSEADNCSQQIS 830
            E+T  VLVSKDGSWKVVSE IDP   SASKTP F+EEKQN+S+P  +N S  +N SQQIS
Sbjct: 340  EDTDHVLVSKDGSWKVVSEIIDPSFGSASKTPEFREEKQNQSNPIGKNLSRDENYSQQIS 399

Query: 829  PTKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVKPNGLSAAMSTINDCKG 650
            PT N++PSRV SMHQNT +S+SLAA+CTNLNDQG  N R      NG+S AMS I DC+G
Sbjct: 400  PTTNVEPSRV-SMHQNTSNSSSLAANCTNLNDQGKKNSRVTGTNSNGMSIAMSPIIDCRG 458

Query: 649  CQLIQKDADLRNQTSGACINTSSSAN------SLSPLQNLVSQNSNAYILPTVEKNGCYQ 488
            C  +QKD +LR Q+S +CINTSSSAN      S+S + +L  QNSNAYILPTVE+NG  Q
Sbjct: 459  CHSLQKDTELRKQSSWSCINTSSSANPLSVQGSVSQVNSLPVQNSNAYILPTVEQNGYCQ 518

Query: 487  NSQGSYTYQNLNKSSNNICEAVRVQQVQLQRDGKVQASLAAQDRQPSVFVPCTRQPRPEG 308
            NS+ ++TYQN   SSNN+CEAV VQQV  QRD ++QASL   +RQPSV   C++Q RPE 
Sbjct: 519  NSRDTHTYQNSKNSSNNVCEAVHVQQVHSQRDDEIQASLNTHNRQPSVLDTCSKQSRPES 578

Query: 307  ERKQNMHEKKNQISHNDGLVELPSEGWRPSGRMRGSLQGQAYSEAYRQFIKQPTQHVRAS 128
            E+KQNMH +KNQ+SH  GLVELPSEGWRPSGRMRGSLQGQAY+EAYR+FIKQPTQ V AS
Sbjct: 579  EQKQNMHREKNQVSHEGGLVELPSEGWRPSGRMRGSLQGQAYAEAYRRFIKQPTQPVGAS 638

Query: 127  ELPPR---LVATLSVAKSQSPNIK 65
            ELP R   L   +SV +   P  K
Sbjct: 639  ELPSRALGLTQAISVLREFRPQGK 662


>XP_019081226.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X4 [Vitis vinifera]
          Length = 694

 Score =  201 bits (510), Expect = 7e-54
 Identities = 164/499 (32%), Positives = 225/499 (45%), Gaps = 64/499 (12%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPGIEMA---LQTSLNCPLSKSRMRTP 1175
            NG Y+IVI  M +I TS TP+LQDY+QPV  +    +E+     + SLNCP+S  R++ P
Sbjct: 175  NGNYVIVIAFMSMISTSNTPELQDYIQPVAVTPDSDLEVIEGQARISLNCPISFKRIKIP 234

Query: 1174 VKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVD 995
            VKGHLCKH QCFDY +++ INSR+P WRCP C++PV   DIRIDQ +VKVL+EV EN VD
Sbjct: 235  VKGHLCKHHQCFDYGNFMEINSRRPSWRCPHCNQPVCNPDIRIDQKMVKVLKEVEENVVD 294

Query: 994  VLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD-PSR-----RNQSEADNCSQQI 833
            V++S DGSWK V E+ID  + S  +    Q E    S+ P++       + + D C   +
Sbjct: 295  VIISPDGSWKPVVESIDHETQSNQQEHSEQCESVGFSNIPAQVVDLTMGEDDEDECLSSL 354

Query: 832  ---------------SPTKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVK 698
                           S  +N  P  VN M +   S +S   D     +Q   +  +  V 
Sbjct: 355  GTEDVKPLSYNLQGSSAAENFLPPGVNYMVEADQSGSSQTEDHVWSAEQFPPSVSNGFVL 414

Query: 697  PNGLSAAMSTINDCKGCQLIQKDADLRNQTSGACINT----SSSANSLSPLQNLVSQNSN 530
            P   S         +          L +  S +   T      +   +S LQN   +  N
Sbjct: 415  PTMSSNGQRNFGVSRTPSSFMSSHVLTDAISPSLWETLGVHRETQMPISSLQNQYFRAGN 474

Query: 529  AYILPTVEKNGCYQNSQGSYTYQNLN-----KSSNNICEAVRVQ--QVQLQRDGKVQASL 371
              I  T   +    N        NL      KS N +  ++R+Q  QVQ +R        
Sbjct: 475  TEIQQTRFGSLSTSNEYRMVCLPNLGATQVIKSRNIVHPSIRIQPGQVQQRRSSVQVTGA 534

Query: 370  AAQDR------QPSVFVPCTRQPRPEGE-----------RKQNMHEKKNQISHNDGLVE- 245
            A Q+R       PS  VP   QP   G            R     E++N +      VE 
Sbjct: 535  AGQERAQMSSMPPS--VPVQLQPARTGTRFSYAMVAEQLRTAAEEERRNMMGRTGMAVEQ 592

Query: 244  -----------LPSEGWRPSGRMRGSLQGQAYSEAYRQFIKQPTQHVRASELPPRLVATL 98
                         +E W+P G MRGSL G+A S A  QF+ QP   + A+ L   L A  
Sbjct: 593  LGTAWSSPGADALAENWQPPGTMRGSLTGEADSAAISQFMLQPPTSLTAT-LESGLPAFW 651

Query: 97   SVAKSQSPNIKASSQTKTQ 41
                S + +    SQT+TQ
Sbjct: 652  PFPSSSTDHNAMYSQTETQ 670


>CBI40413.3 unnamed protein product, partial [Vitis vinifera]
          Length = 776

 Score =  201 bits (512), Expect = 1e-53
 Identities = 166/497 (33%), Positives = 224/497 (45%), Gaps = 62/497 (12%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPV-VASGGPGIEMALQTSLNCPLSKSRMRTPVK 1169
            NG Y+IVI  M +I TS TP+LQDY+QPV V      IE   + SLNCP+S  R++ PVK
Sbjct: 259  NGNYVIVIAFMSMISTSNTPELQDYIQPVAVTPDSEVIEGQARISLNCPISFKRIKIPVK 318

Query: 1168 GHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVDVL 989
            GHLCKH QCFDY +++ INSR+P WRCP C++PV   DIRIDQ +VKVL+EV EN VDV+
Sbjct: 319  GHLCKHHQCFDYGNFMEINSRRPSWRCPHCNQPVCNPDIRIDQKMVKVLKEVEENVVDVI 378

Query: 988  VSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD-PSR-----RNQSEADNCSQQI-- 833
            +S DGSWK V E+ID  + S  +    Q E    S+ P++       + + D C   +  
Sbjct: 379  ISPDGSWKPVVESIDHETQSNQQEHSEQCESVGFSNIPAQVVDLTMGEDDEDECLSSLGT 438

Query: 832  -------------SPTKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVKPN 692
                         S  +N  P  VN M +   S +S   D     +Q   +  +  V P 
Sbjct: 439  EDVKPLSYNLQGSSAAENFLPPGVNYMVEADQSGSSQTEDHVWSAEQFPPSVSNGFVLPT 498

Query: 691  GLSAAMSTINDCKGCQLIQKDADLRNQTSGACINT----SSSANSLSPLQNLVSQNSNAY 524
              S         +          L +  S +   T      +   +S LQN   +  N  
Sbjct: 499  MSSNGQRNFGVSRTPSSFMSSHVLTDAISPSLWETLGVHRETQMPISSLQNQYFRAGNTE 558

Query: 523  ILPTVEKNGCYQNSQGSYTYQNLN-----KSSNNICEAVRVQ--QVQLQRDGKVQASLAA 365
            I  T   +    N        NL      KS N +  ++R+Q  QVQ +R        A 
Sbjct: 559  IQQTRFGSLSTSNEYRMVCLPNLGATQVIKSRNIVHPSIRIQPGQVQQRRSSVQVTGAAG 618

Query: 364  QDR------QPSVFVPCTRQPRPEGE-----------RKQNMHEKKNQISHNDGLVE--- 245
            Q+R       PS  VP   QP   G            R     E++N +      VE   
Sbjct: 619  QERAQMSSMPPS--VPVQLQPARTGTRFSYAMVAEQLRTAAEEERRNMMGRTGMAVEQLG 676

Query: 244  ---------LPSEGWRPSGRMRGSLQGQAYSEAYRQFIKQPTQHVRASELPPRLVATLSV 92
                       +E W+P G MRGSL G+A S A  QF+ QP   + A+ L   L A    
Sbjct: 677  TAWSSPGADALAENWQPPGTMRGSLTGEADSAAISQFMLQPPTSLTAT-LESGLPAFWPF 735

Query: 91   AKSQSPNIKASSQTKTQ 41
              S + +    SQT+TQ
Sbjct: 736  PSSSTDHNAMYSQTETQ 752


>XP_019081225.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X3 [Vitis vinifera]
          Length = 756

 Score =  201 bits (510), Expect = 2e-53
 Identities = 164/499 (32%), Positives = 225/499 (45%), Gaps = 64/499 (12%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPGIEMA---LQTSLNCPLSKSRMRTP 1175
            NG Y+IVI  M +I TS TP+LQDY+QPV  +    +E+     + SLNCP+S  R++ P
Sbjct: 237  NGNYVIVIAFMSMISTSNTPELQDYIQPVAVTPDSDLEVIEGQARISLNCPISFKRIKIP 296

Query: 1174 VKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVD 995
            VKGHLCKH QCFDY +++ INSR+P WRCP C++PV   DIRIDQ +VKVL+EV EN VD
Sbjct: 297  VKGHLCKHHQCFDYGNFMEINSRRPSWRCPHCNQPVCNPDIRIDQKMVKVLKEVEENVVD 356

Query: 994  VLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD-PSR-----RNQSEADNCSQQI 833
            V++S DGSWK V E+ID  + S  +    Q E    S+ P++       + + D C   +
Sbjct: 357  VIISPDGSWKPVVESIDHETQSNQQEHSEQCESVGFSNIPAQVVDLTMGEDDEDECLSSL 416

Query: 832  ---------------SPTKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVK 698
                           S  +N  P  VN M +   S +S   D     +Q   +  +  V 
Sbjct: 417  GTEDVKPLSYNLQGSSAAENFLPPGVNYMVEADQSGSSQTEDHVWSAEQFPPSVSNGFVL 476

Query: 697  PNGLSAAMSTINDCKGCQLIQKDADLRNQTSGACINT----SSSANSLSPLQNLVSQNSN 530
            P   S         +          L +  S +   T      +   +S LQN   +  N
Sbjct: 477  PTMSSNGQRNFGVSRTPSSFMSSHVLTDAISPSLWETLGVHRETQMPISSLQNQYFRAGN 536

Query: 529  AYILPTVEKNGCYQNSQGSYTYQNLN-----KSSNNICEAVRVQ--QVQLQRDGKVQASL 371
              I  T   +    N        NL      KS N +  ++R+Q  QVQ +R        
Sbjct: 537  TEIQQTRFGSLSTSNEYRMVCLPNLGATQVIKSRNIVHPSIRIQPGQVQQRRSSVQVTGA 596

Query: 370  AAQDR------QPSVFVPCTRQPRPEGE-----------RKQNMHEKKNQISHNDGLVE- 245
            A Q+R       PS  VP   QP   G            R     E++N +      VE 
Sbjct: 597  AGQERAQMSSMPPS--VPVQLQPARTGTRFSYAMVAEQLRTAAEEERRNMMGRTGMAVEQ 654

Query: 244  -----------LPSEGWRPSGRMRGSLQGQAYSEAYRQFIKQPTQHVRASELPPRLVATL 98
                         +E W+P G MRGSL G+A S A  QF+ QP   + A+ L   L A  
Sbjct: 655  LGTAWSSPGADALAENWQPPGTMRGSLTGEADSAAISQFMLQPPTSLTAT-LESGLPAFW 713

Query: 97   SVAKSQSPNIKASSQTKTQ 41
                S + +    SQT+TQ
Sbjct: 714  PFPSSSTDHNAMYSQTETQ 732


>XP_019081224.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X2 [Vitis vinifera]
          Length = 756

 Score =  201 bits (510), Expect = 2e-53
 Identities = 164/499 (32%), Positives = 225/499 (45%), Gaps = 64/499 (12%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPGIEMA---LQTSLNCPLSKSRMRTP 1175
            NG Y+IVI  M +I TS TP+LQDY+QPV  +    +E+     + SLNCP+S  R++ P
Sbjct: 237  NGNYVIVIAFMSMISTSNTPELQDYIQPVAVTPDSDLEVIEGQARISLNCPISFKRIKIP 296

Query: 1174 VKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVD 995
            VKGHLCKH QCFDY +++ INSR+P WRCP C++PV   DIRIDQ +VKVL+EV EN VD
Sbjct: 297  VKGHLCKHHQCFDYGNFMEINSRRPSWRCPHCNQPVCNPDIRIDQKMVKVLKEVEENVVD 356

Query: 994  VLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD-PSR-----RNQSEADNCSQQI 833
            V++S DGSWK V E+ID  + S  +    Q E    S+ P++       + + D C   +
Sbjct: 357  VIISPDGSWKPVVESIDHETQSNQQEHSEQCESVGFSNIPAQVVDLTMGEDDEDECLSSL 416

Query: 832  ---------------SPTKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVK 698
                           S  +N  P  VN M +   S +S   D     +Q   +  +  V 
Sbjct: 417  GTEDVKPLSYNLQGSSAAENFLPPGVNYMVEADQSGSSQTEDHVWSAEQFPPSVSNGFVL 476

Query: 697  PNGLSAAMSTINDCKGCQLIQKDADLRNQTSGACINT----SSSANSLSPLQNLVSQNSN 530
            P   S         +          L +  S +   T      +   +S LQN   +  N
Sbjct: 477  PTMSSNGQRNFGVSRTPSSFMSSHVLTDAISPSLWETLGVHRETQMPISSLQNQYFRAGN 536

Query: 529  AYILPTVEKNGCYQNSQGSYTYQNLN-----KSSNNICEAVRVQ--QVQLQRDGKVQASL 371
              I  T   +    N        NL      KS N +  ++R+Q  QVQ +R        
Sbjct: 537  TEIQQTRFGSLSTSNEYRMVCLPNLGATQVIKSRNIVHPSIRIQPGQVQQRRSSVQVTGA 596

Query: 370  AAQDR------QPSVFVPCTRQPRPEGE-----------RKQNMHEKKNQISHNDGLVE- 245
            A Q+R       PS  VP   QP   G            R     E++N +      VE 
Sbjct: 597  AGQERAQMSSMPPS--VPVQLQPARTGTRFSYAMVAEQLRTAAEEERRNMMGRTGMAVEQ 654

Query: 244  -----------LPSEGWRPSGRMRGSLQGQAYSEAYRQFIKQPTQHVRASELPPRLVATL 98
                         +E W+P G MRGSL G+A S A  QF+ QP   + A+ L   L A  
Sbjct: 655  LGTAWSSPGADALAENWQPPGTMRGSLTGEADSAAISQFMLQPPTSLTAT-LESGLPAFW 713

Query: 97   SVAKSQSPNIKASSQTKTQ 41
                S + +    SQT+TQ
Sbjct: 714  PFPSSSTDHNAMYSQTETQ 732


>XP_010661811.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
            XP_010661814.1 PREDICTED: E4 SUMO-protein ligase PIAL2
            isoform X1 [Vitis vinifera] XP_010661817.1 PREDICTED: E4
            SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
            XP_019081218.1 PREDICTED: E4 SUMO-protein ligase PIAL2
            isoform X1 [Vitis vinifera] XP_019081219.1 PREDICTED: E4
            SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
            XP_019081220.1 PREDICTED: E4 SUMO-protein ligase PIAL2
            isoform X1 [Vitis vinifera] XP_019081221.1 PREDICTED: E4
            SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
            XP_019081222.1 PREDICTED: E4 SUMO-protein ligase PIAL2
            isoform X1 [Vitis vinifera] XP_019081223.1 PREDICTED: E4
            SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
          Length = 778

 Score =  201 bits (510), Expect = 2e-53
 Identities = 164/499 (32%), Positives = 225/499 (45%), Gaps = 64/499 (12%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPGIEMA---LQTSLNCPLSKSRMRTP 1175
            NG Y+IVI  M +I TS TP+LQDY+QPV  +    +E+     + SLNCP+S  R++ P
Sbjct: 259  NGNYVIVIAFMSMISTSNTPELQDYIQPVAVTPDSDLEVIEGQARISLNCPISFKRIKIP 318

Query: 1174 VKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVD 995
            VKGHLCKH QCFDY +++ INSR+P WRCP C++PV   DIRIDQ +VKVL+EV EN VD
Sbjct: 319  VKGHLCKHHQCFDYGNFMEINSRRPSWRCPHCNQPVCNPDIRIDQKMVKVLKEVEENVVD 378

Query: 994  VLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD-PSR-----RNQSEADNCSQQI 833
            V++S DGSWK V E+ID  + S  +    Q E    S+ P++       + + D C   +
Sbjct: 379  VIISPDGSWKPVVESIDHETQSNQQEHSEQCESVGFSNIPAQVVDLTMGEDDEDECLSSL 438

Query: 832  ---------------SPTKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVK 698
                           S  +N  P  VN M +   S +S   D     +Q   +  +  V 
Sbjct: 439  GTEDVKPLSYNLQGSSAAENFLPPGVNYMVEADQSGSSQTEDHVWSAEQFPPSVSNGFVL 498

Query: 697  PNGLSAAMSTINDCKGCQLIQKDADLRNQTSGACINT----SSSANSLSPLQNLVSQNSN 530
            P   S         +          L +  S +   T      +   +S LQN   +  N
Sbjct: 499  PTMSSNGQRNFGVSRTPSSFMSSHVLTDAISPSLWETLGVHRETQMPISSLQNQYFRAGN 558

Query: 529  AYILPTVEKNGCYQNSQGSYTYQNLN-----KSSNNICEAVRVQ--QVQLQRDGKVQASL 371
              I  T   +    N        NL      KS N +  ++R+Q  QVQ +R        
Sbjct: 559  TEIQQTRFGSLSTSNEYRMVCLPNLGATQVIKSRNIVHPSIRIQPGQVQQRRSSVQVTGA 618

Query: 370  AAQDR------QPSVFVPCTRQPRPEGE-----------RKQNMHEKKNQISHNDGLVE- 245
            A Q+R       PS  VP   QP   G            R     E++N +      VE 
Sbjct: 619  AGQERAQMSSMPPS--VPVQLQPARTGTRFSYAMVAEQLRTAAEEERRNMMGRTGMAVEQ 676

Query: 244  -----------LPSEGWRPSGRMRGSLQGQAYSEAYRQFIKQPTQHVRASELPPRLVATL 98
                         +E W+P G MRGSL G+A S A  QF+ QP   + A+ L   L A  
Sbjct: 677  LGTAWSSPGADALAENWQPPGTMRGSLTGEADSAAISQFMLQPPTSLTAT-LESGLPAFW 735

Query: 97   SVAKSQSPNIKASSQTKTQ 41
                S + +    SQT+TQ
Sbjct: 736  PFPSSSTDHNAMYSQTETQ 754


>XP_018841222.1 PREDICTED: E4 SUMO-protein ligase PIAL1-like isoform X3 [Juglans
            regia]
          Length = 1083

 Score =  182 bits (462), Expect = 2e-46
 Identities = 146/493 (29%), Positives = 218/493 (44%), Gaps = 108/493 (21%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIP-TSGTPQLQDYVQPVVASGGPG---IEMALQTSLNCPLSKSRMRT 1178
            NG Y+I I  M ++P T  TP LQDYVQPVV   G     IE   + SLNCP+S  R++T
Sbjct: 241  NGNYLITIAFMSMMPLTYETPSLQDYVQPVVGPYGSDSEIIERPSRISLNCPISHKRIKT 300

Query: 1177 PVKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTV 998
            PVKGHLCKH QCFDY++++ +N+R+P W CP C++PV  +DIRID+++ K+L E  EN V
Sbjct: 301  PVKGHLCKHYQCFDYENFMEMNARRPLWCCPYCNQPVCHTDIRIDEDIAKILGETEENVV 360

Query: 997  DVLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD--------------------- 881
            DV++S DGSW  V +N D    +   TP  Q+E+  + D                     
Sbjct: 361  DVMISADGSWMSVLQNKDHADKTGHGTPSCQQERPRQCDSDGFSCLPDEFSDLTKEGANA 420

Query: 880  -------PSRRNQSEADNC------SQQISPTKNIQPSRVNSMHQNTCSSNSLAADCTNL 740
                     RR+  +  +C       +++     +      S  +N  S   ++     L
Sbjct: 421  MHVYGGGTGRRHLLDNPSCIPRNVVQKEVKNCDRLDQHGSCSTEENIYSRIPISISAVPL 480

Query: 739  NDQGNNN-----PRSMNVKPNGLSAAM--STINDCKGCQLIQKDADLRNQTSGACINT-- 587
               G+ +     P S     N + A +  +T++     Q +    D   Q     +N+  
Sbjct: 481  GSVGSTSTWAGTPGSSKSSCNSILAPVLTNTLSPVLNQQSLHGGLDREQQLLRPLVNSLP 540

Query: 586  -----SSSANSLSPLQNLVSQNSN---------AYILPTVEKNGCYQNSQGSYTYQNLNK 449
                 SSS ++ S +QN      N         A I PT    G + +S+ S  +QN  +
Sbjct: 541  VSDMASSSMHNNSSIQNQEVNKDNYVSGQDVQQADIFPTQGLGGHHISSKSSSGHQNSLQ 600

Query: 448  SS-----------NNICEAVRVQQVQLQRDG----------------KVQASLAAQDRQP 350
            SS           N++  +  V+ VQ Q+ G                K  A   A+  + 
Sbjct: 601  SSASHPSAMTPSLNSVPPSTVVKPVQFQQGGGSGQAMHNVNSTQTPLKSTAQPMAEKSRT 660

Query: 349  SVFVPCTRQP--------------RPEGERKQNMHEKKNQISH-----NDGLVELPS-EG 230
             ++VP   QP              RP G  K+      ++I+H      +G   L S + 
Sbjct: 661  PLYVPVQLQPSGTDLSSSLSTVAERPRGTSKKG--GSVSEITHPQRLQTEGSATLTSAQN 718

Query: 229  WRPSGRMRGSLQG 191
            W+PSGRM GSL G
Sbjct: 719  WQPSGRMEGSLMG 731


>XP_018841221.1 PREDICTED: E4 SUMO-protein ligase PIAL1-like isoform X2 [Juglans
            regia]
          Length = 1105

 Score =  182 bits (462), Expect = 2e-46
 Identities = 146/493 (29%), Positives = 218/493 (44%), Gaps = 108/493 (21%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIP-TSGTPQLQDYVQPVVASGGPG---IEMALQTSLNCPLSKSRMRT 1178
            NG Y+I I  M ++P T  TP LQDYVQPVV   G     IE   + SLNCP+S  R++T
Sbjct: 263  NGNYLITIAFMSMMPLTYETPSLQDYVQPVVGPYGSDSEIIERPSRISLNCPISHKRIKT 322

Query: 1177 PVKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTV 998
            PVKGHLCKH QCFDY++++ +N+R+P W CP C++PV  +DIRID+++ K+L E  EN V
Sbjct: 323  PVKGHLCKHYQCFDYENFMEMNARRPLWCCPYCNQPVCHTDIRIDEDIAKILGETEENVV 382

Query: 997  DVLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD--------------------- 881
            DV++S DGSW  V +N D    +   TP  Q+E+  + D                     
Sbjct: 383  DVMISADGSWMSVLQNKDHADKTGHGTPSCQQERPRQCDSDGFSCLPDEFSDLTKEGANA 442

Query: 880  -------PSRRNQSEADNC------SQQISPTKNIQPSRVNSMHQNTCSSNSLAADCTNL 740
                     RR+  +  +C       +++     +      S  +N  S   ++     L
Sbjct: 443  MHVYGGGTGRRHLLDNPSCIPRNVVQKEVKNCDRLDQHGSCSTEENIYSRIPISISAVPL 502

Query: 739  NDQGNNN-----PRSMNVKPNGLSAAM--STINDCKGCQLIQKDADLRNQTSGACINT-- 587
               G+ +     P S     N + A +  +T++     Q +    D   Q     +N+  
Sbjct: 503  GSVGSTSTWAGTPGSSKSSCNSILAPVLTNTLSPVLNQQSLHGGLDREQQLLRPLVNSLP 562

Query: 586  -----SSSANSLSPLQNLVSQNSN---------AYILPTVEKNGCYQNSQGSYTYQNLNK 449
                 SSS ++ S +QN      N         A I PT    G + +S+ S  +QN  +
Sbjct: 563  VSDMASSSMHNNSSIQNQEVNKDNYVSGQDVQQADIFPTQGLGGHHISSKSSSGHQNSLQ 622

Query: 448  SS-----------NNICEAVRVQQVQLQRDG----------------KVQASLAAQDRQP 350
            SS           N++  +  V+ VQ Q+ G                K  A   A+  + 
Sbjct: 623  SSASHPSAMTPSLNSVPPSTVVKPVQFQQGGGSGQAMHNVNSTQTPLKSTAQPMAEKSRT 682

Query: 349  SVFVPCTRQP--------------RPEGERKQNMHEKKNQISH-----NDGLVELPS-EG 230
             ++VP   QP              RP G  K+      ++I+H      +G   L S + 
Sbjct: 683  PLYVPVQLQPSGTDLSSSLSTVAERPRGTSKKG--GSVSEITHPQRLQTEGSATLTSAQN 740

Query: 229  WRPSGRMRGSLQG 191
            W+PSGRM GSL G
Sbjct: 741  WQPSGRMEGSLMG 753


>XP_018841220.1 PREDICTED: E4 SUMO-protein ligase PIAL1-like isoform X1 [Juglans
            regia]
          Length = 1105

 Score =  182 bits (462), Expect = 2e-46
 Identities = 146/493 (29%), Positives = 218/493 (44%), Gaps = 108/493 (21%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIP-TSGTPQLQDYVQPVVASGGPG---IEMALQTSLNCPLSKSRMRT 1178
            NG Y+I I  M ++P T  TP LQDYVQPVV   G     IE   + SLNCP+S  R++T
Sbjct: 263  NGNYLITIAFMSMMPLTYETPSLQDYVQPVVGPYGSDSEIIERPSRISLNCPISHKRIKT 322

Query: 1177 PVKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTV 998
            PVKGHLCKH QCFDY++++ +N+R+P W CP C++PV  +DIRID+++ K+L E  EN V
Sbjct: 323  PVKGHLCKHYQCFDYENFMEMNARRPLWCCPYCNQPVCHTDIRIDEDIAKILGETEENVV 382

Query: 997  DVLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD--------------------- 881
            DV++S DGSW  V +N D    +   TP  Q+E+  + D                     
Sbjct: 383  DVMISADGSWMSVLQNKDHADKTGHGTPSCQQERPRQCDSDGFSCLPDEFSDLTKEGANA 442

Query: 880  -------PSRRNQSEADNC------SQQISPTKNIQPSRVNSMHQNTCSSNSLAADCTNL 740
                     RR+  +  +C       +++     +      S  +N  S   ++     L
Sbjct: 443  MHVYGGGTGRRHLLDNPSCIPRNVVQKEVKNCDRLDQHGSCSTEENIYSRIPISISAVPL 502

Query: 739  NDQGNNN-----PRSMNVKPNGLSAAM--STINDCKGCQLIQKDADLRNQTSGACINT-- 587
               G+ +     P S     N + A +  +T++     Q +    D   Q     +N+  
Sbjct: 503  GSVGSTSTWAGTPGSSKSSCNSILAPVLTNTLSPVLNQQSLHGGLDREQQLLRPLVNSLP 562

Query: 586  -----SSSANSLSPLQNLVSQNSN---------AYILPTVEKNGCYQNSQGSYTYQNLNK 449
                 SSS ++ S +QN      N         A I PT    G + +S+ S  +QN  +
Sbjct: 563  VSDMASSSMHNNSSIQNQEVNKDNYVSGQDVQQADIFPTQGLGGHHISSKSSSGHQNSLQ 622

Query: 448  SS-----------NNICEAVRVQQVQLQRDG----------------KVQASLAAQDRQP 350
            SS           N++  +  V+ VQ Q+ G                K  A   A+  + 
Sbjct: 623  SSASHPSAMTPSLNSVPPSTVVKPVQFQQGGGSGQAMHNVNSTQTPLKSTAQPMAEKSRT 682

Query: 349  SVFVPCTRQP--------------RPEGERKQNMHEKKNQISH-----NDGLVELPS-EG 230
             ++VP   QP              RP G  K+      ++I+H      +G   L S + 
Sbjct: 683  PLYVPVQLQPSGTDLSSSLSTVAERPRGTSKKG--GSVSEITHPQRLQTEGSATLTSAQN 740

Query: 229  WRPSGRMRGSLQG 191
            W+PSGRM GSL G
Sbjct: 741  WQPSGRMEGSLMG 753


>KDP25375.1 hypothetical protein JCGZ_20531 [Jatropha curcas]
          Length = 741

 Score =  180 bits (457), Expect = 3e-46
 Identities = 138/459 (30%), Positives = 220/459 (47%), Gaps = 33/459 (7%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPG---IEMALQTSLNCPLSKSRMRTP 1175
            NG YII +G M  +P  GT  L DYVQ   A   P    IE   + SLNCP+S SR+RTP
Sbjct: 102  NGNYIIAVGFMSEVPLPGTSVLPDYVQSDAAVADPDSDIIEGPSRVSLNCPISYSRIRTP 161

Query: 1174 VKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVD 995
            +KGH CKH QCFD+ +++ INSR+P WRCP C++ V  SDIR+DQN+VKVL+EVGEN V+
Sbjct: 162  IKGHSCKHLQCFDFSNFIEINSRRPSWRCPHCNQHVCYSDIRVDQNMVKVLKEVGENVVN 221

Query: 994  VLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD------PSRRNQSEADN--CSQ 839
            V++S DGSWK V E+ +    S  +    Q+E   + +      PS  + +E D+   + 
Sbjct: 222  VIISVDGSWKAVMESDENMDHSCKEPVNCQKETPEQQEPATSLIPSVLDLTEDDDRMDAM 281

Query: 838  QISPTKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVKPNGLSAAMSTIND 659
              S  ++ +P + +   Q+  ++ ++ +  TN+ DQ N + + M+   +G       +ND
Sbjct: 282  STSDVEDRKPFQSSFQSQSVATNLTMPSHDTNVFDQ-NASDQLMDELLSGY------LND 334

Query: 658  CKGCQL----IQKDADLRNQTSGACINTSSSANSLSPLQNLVSQNSNAYILPTVEK---- 503
              G  +     Q    +   + G  + +S S++++SP+ N          L ++ +    
Sbjct: 335  GSGASIAGLCTQTVNGIPESSPGNAMTSSVSSDAISPVLNCNFGGHGNNSLTSLTQTQFS 394

Query: 502  ---------NGCYQNSQGSYTYQNLNKSSNNICEAVRVQQVQLQRDGKVQASLAAQDRQP 350
                     N    N  G +T  N+ +  N    AV+   V  Q   + Q S    + + 
Sbjct: 395  ASGDLHQLVNAAVNNEYGRFT--NIPRHVNRTSIAVQALAVASQISAQQQRSRTNLNGEN 452

Query: 349  SVFVPCTRQPRPEGERKQNMHEKKNQISHNDGLVELPSE-GWRPSGRMRGS---LQGQAY 182
                P T QP        N     +  +H  GL  + S     P+G+ R     + GQ++
Sbjct: 453  PNGAPQTSQPTLPAANGFNTASISHTNAH-QGLNRVASPIPHYPTGQNRQDRPYVSGQSF 511

Query: 181  SEAY-RQFIKQPTQHVRASELPPRLVATLSVAKSQSPNI 68
             +    Q ++ P     +  L       +  ++SQSP +
Sbjct: 512  QQVNPLQLLRAPRFRNESQNLHQLQALQMPQSRSQSPTV 550


>XP_012086815.1 PREDICTED: uncharacterized protein LOC105645744 isoform X2 [Jatropha
            curcas]
          Length = 906

 Score =  180 bits (457), Expect = 8e-46
 Identities = 138/459 (30%), Positives = 220/459 (47%), Gaps = 33/459 (7%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPG---IEMALQTSLNCPLSKSRMRTP 1175
            NG YII +G M  +P  GT  L DYVQ   A   P    IE   + SLNCP+S SR+RTP
Sbjct: 267  NGNYIIAVGFMSEVPLPGTSVLPDYVQSDAAVADPDSDIIEGPSRVSLNCPISYSRIRTP 326

Query: 1174 VKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVD 995
            +KGH CKH QCFD+ +++ INSR+P WRCP C++ V  SDIR+DQN+VKVL+EVGEN V+
Sbjct: 327  IKGHSCKHLQCFDFSNFIEINSRRPSWRCPHCNQHVCYSDIRVDQNMVKVLKEVGENVVN 386

Query: 994  VLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD------PSRRNQSEADN--CSQ 839
            V++S DGSWK V E+ +    S  +    Q+E   + +      PS  + +E D+   + 
Sbjct: 387  VIISVDGSWKAVMESDENMDHSCKEPVNCQKETPEQQEPATSLIPSVLDLTEDDDRMDAM 446

Query: 838  QISPTKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVKPNGLSAAMSTIND 659
              S  ++ +P + +   Q+  ++ ++ +  TN+ DQ N + + M+   +G       +ND
Sbjct: 447  STSDVEDRKPFQSSFQSQSVATNLTMPSHDTNVFDQ-NASDQLMDELLSGY------LND 499

Query: 658  CKGCQL----IQKDADLRNQTSGACINTSSSANSLSPLQNLVSQNSNAYILPTVEK---- 503
              G  +     Q    +   + G  + +S S++++SP+ N          L ++ +    
Sbjct: 500  GSGASIAGLCTQTVNGIPESSPGNAMTSSVSSDAISPVLNCNFGGHGNNSLTSLTQTQFS 559

Query: 502  ---------NGCYQNSQGSYTYQNLNKSSNNICEAVRVQQVQLQRDGKVQASLAAQDRQP 350
                     N    N  G +T  N+ +  N    AV+   V  Q   + Q S    + + 
Sbjct: 560  ASGDLHQLVNAAVNNEYGRFT--NIPRHVNRTSIAVQALAVASQISAQQQRSRTNLNGEN 617

Query: 349  SVFVPCTRQPRPEGERKQNMHEKKNQISHNDGLVELPSE-GWRPSGRMRGS---LQGQAY 182
                P T QP        N     +  +H  GL  + S     P+G+ R     + GQ++
Sbjct: 618  PNGAPQTSQPTLPAANGFNTASISHTNAH-QGLNRVASPIPHYPTGQNRQDRPYVSGQSF 676

Query: 181  SEAY-RQFIKQPTQHVRASELPPRLVATLSVAKSQSPNI 68
             +    Q ++ P     +  L       +  ++SQSP +
Sbjct: 677  QQVNPLQLLRAPRFRNESQNLHQLQALQMPQSRSQSPTV 715


>XP_012086814.1 PREDICTED: uncharacterized protein LOC105645744 isoform X1 [Jatropha
            curcas]
          Length = 908

 Score =  180 bits (457), Expect = 8e-46
 Identities = 138/459 (30%), Positives = 220/459 (47%), Gaps = 33/459 (7%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPG---IEMALQTSLNCPLSKSRMRTP 1175
            NG YII +G M  +P  GT  L DYVQ   A   P    IE   + SLNCP+S SR+RTP
Sbjct: 269  NGNYIIAVGFMSEVPLPGTSVLPDYVQSDAAVADPDSDIIEGPSRVSLNCPISYSRIRTP 328

Query: 1174 VKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVD 995
            +KGH CKH QCFD+ +++ INSR+P WRCP C++ V  SDIR+DQN+VKVL+EVGEN V+
Sbjct: 329  IKGHSCKHLQCFDFSNFIEINSRRPSWRCPHCNQHVCYSDIRVDQNMVKVLKEVGENVVN 388

Query: 994  VLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD------PSRRNQSEADN--CSQ 839
            V++S DGSWK V E+ +    S  +    Q+E   + +      PS  + +E D+   + 
Sbjct: 389  VIISVDGSWKAVMESDENMDHSCKEPVNCQKETPEQQEPATSLIPSVLDLTEDDDRMDAM 448

Query: 838  QISPTKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVKPNGLSAAMSTIND 659
              S  ++ +P + +   Q+  ++ ++ +  TN+ DQ N + + M+   +G       +ND
Sbjct: 449  STSDVEDRKPFQSSFQSQSVATNLTMPSHDTNVFDQ-NASDQLMDELLSGY------LND 501

Query: 658  CKGCQL----IQKDADLRNQTSGACINTSSSANSLSPLQNLVSQNSNAYILPTVEK---- 503
              G  +     Q    +   + G  + +S S++++SP+ N          L ++ +    
Sbjct: 502  GSGASIAGLCTQTVNGIPESSPGNAMTSSVSSDAISPVLNCNFGGHGNNSLTSLTQTQFS 561

Query: 502  ---------NGCYQNSQGSYTYQNLNKSSNNICEAVRVQQVQLQRDGKVQASLAAQDRQP 350
                     N    N  G +T  N+ +  N    AV+   V  Q   + Q S    + + 
Sbjct: 562  ASGDLHQLVNAAVNNEYGRFT--NIPRHVNRTSIAVQALAVASQISAQQQRSRTNLNGEN 619

Query: 349  SVFVPCTRQPRPEGERKQNMHEKKNQISHNDGLVELPSE-GWRPSGRMRGS---LQGQAY 182
                P T QP        N     +  +H  GL  + S     P+G+ R     + GQ++
Sbjct: 620  PNGAPQTSQPTLPAANGFNTASISHTNAH-QGLNRVASPIPHYPTGQNRQDRPYVSGQSF 678

Query: 181  SEAY-RQFIKQPTQHVRASELPPRLVATLSVAKSQSPNI 68
             +    Q ++ P     +  L       +  ++SQSP +
Sbjct: 679  QQVNPLQLLRAPRFRNESQNLHQLQALQMPQSRSQSPTV 717


>XP_010661825.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X2 [Vitis vinifera]
          Length = 859

 Score =  176 bits (446), Expect = 2e-44
 Identities = 85/168 (50%), Positives = 111/168 (66%), Gaps = 3/168 (1%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPGIEMA---LQTSLNCPLSKSRMRTP 1175
            NG Y+I+I  M VI TSG P+LQ+Y+QPV  +    +E+     + SLNCP+S  R+  P
Sbjct: 233  NGNYVIIIAFMSVISTSGIPELQEYIQPVAVTSDSDLEIIEGQARISLNCPISFRRINIP 292

Query: 1174 VKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVD 995
            VKGHLCKH QCFDY +++ INSR+P WRCP C++ V   DIRIDQN+VK+LREVGEN VD
Sbjct: 293  VKGHLCKHHQCFDYGNFIEINSRRPSWRCPHCNQSVCNPDIRIDQNMVKILREVGENVVD 352

Query: 994  VLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSDPSRRNQSEAD 851
            V++S DGSWK V E+ID        T    +E   + +  R +   AD
Sbjct: 353  VIISPDGSWKPVVESIDHAEQLYDATQSNWQENTKQCESVRLSSIAAD 400



 Score = 69.3 bits (168), Expect = 7e-09
 Identities = 61/184 (33%), Positives = 81/184 (44%), Gaps = 41/184 (22%)
 Frame = -3

Query: 469  TYQNLNKSSNNICEAVRVQQVQLQ-RDGKVQASLAAQDRQ-----------------PSV 344
            T Q +N+  NN+  ++ VQ +Q Q R G  Q + +  +RQ                 PSV
Sbjct: 654  TTQAMNEPRNNVHPSIHVQSMQRQQRSGGSQVTGSVPNRQSPHAAAAQQTVQVSRSPPSV 713

Query: 343  FVPC--------------TRQPRPEGERKQNMHEKKNQISHNDGLVELPS-EGWRPSGRM 209
             V                  Q R  GE+++N+          D    LP+ E WRPSGRM
Sbjct: 714  PVQLRPARTGTAFSVGMVAEQLRTAGEQRRNILGTAWSTPRPDASAALPTDENWRPSGRM 773

Query: 208  RGSLQGQAYSEAYRQFIKQPTQHVRA--------SELPPRLVATLSVAKSQSPNIKASSQ 53
            RGSL G+AYS A  QF+ QPTQ  +A        S LP  L A      S + +  A SQ
Sbjct: 774  RGSLTGEAYSAALNQFMLQPTQPTQAPLPPTSLPSNLPGGLPAFRLFPSSSNEHNAAPSQ 833

Query: 52   TKTQ 41
            T+ Q
Sbjct: 834  TEPQ 837


>XP_010661824.1 PREDICTED: E4 SUMO-protein ligase PIAL2 isoform X1 [Vitis vinifera]
          Length = 883

 Score =  176 bits (446), Expect = 2e-44
 Identities = 85/168 (50%), Positives = 111/168 (66%), Gaps = 3/168 (1%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPGIEMA---LQTSLNCPLSKSRMRTP 1175
            NG Y+I+I  M VI TSG P+LQ+Y+QPV  +    +E+     + SLNCP+S  R+  P
Sbjct: 257  NGNYVIIIAFMSVISTSGIPELQEYIQPVAVTSDSDLEIIEGQARISLNCPISFRRINIP 316

Query: 1174 VKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVD 995
            VKGHLCKH QCFDY +++ INSR+P WRCP C++ V   DIRIDQN+VK+LREVGEN VD
Sbjct: 317  VKGHLCKHHQCFDYGNFIEINSRRPSWRCPHCNQSVCNPDIRIDQNMVKILREVGENVVD 376

Query: 994  VLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSDPSRRNQSEAD 851
            V++S DGSWK V E+ID        T    +E   + +  R +   AD
Sbjct: 377  VIISPDGSWKPVVESIDHAEQLYDATQSNWQENTKQCESVRLSSIAAD 424



 Score = 69.3 bits (168), Expect = 7e-09
 Identities = 61/184 (33%), Positives = 81/184 (44%), Gaps = 41/184 (22%)
 Frame = -3

Query: 469  TYQNLNKSSNNICEAVRVQQVQLQ-RDGKVQASLAAQDRQ-----------------PSV 344
            T Q +N+  NN+  ++ VQ +Q Q R G  Q + +  +RQ                 PSV
Sbjct: 678  TTQAMNEPRNNVHPSIHVQSMQRQQRSGGSQVTGSVPNRQSPHAAAAQQTVQVSRSPPSV 737

Query: 343  FVPC--------------TRQPRPEGERKQNMHEKKNQISHNDGLVELPS-EGWRPSGRM 209
             V                  Q R  GE+++N+          D    LP+ E WRPSGRM
Sbjct: 738  PVQLRPARTGTAFSVGMVAEQLRTAGEQRRNILGTAWSTPRPDASAALPTDENWRPSGRM 797

Query: 208  RGSLQGQAYSEAYRQFIKQPTQHVRA--------SELPPRLVATLSVAKSQSPNIKASSQ 53
            RGSL G+AYS A  QF+ QPTQ  +A        S LP  L A      S + +  A SQ
Sbjct: 798  RGSLTGEAYSAALNQFMLQPTQPTQAPLPPTSLPSNLPGGLPAFRLFPSSSNEHNAAPSQ 857

Query: 52   TKTQ 41
            T+ Q
Sbjct: 858  TEPQ 861


>XP_019052072.1 PREDICTED: E4 SUMO-protein ligase PIAL1-like isoform X2 [Nelumbo
            nucifera]
          Length = 925

 Score =  176 bits (446), Expect = 2e-44
 Identities = 85/163 (52%), Positives = 115/163 (70%), Gaps = 1/163 (0%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVAS-GGPGIEMALQTSLNCPLSKSRMRTPVK 1169
            N  YII +  M  + +S  P+LQDYVQP V+S     IE   + SLNCP+S++R++TPVK
Sbjct: 247  NAKYIIAVAFMSDVASSDNPELQDYVQPAVSSLDSEVIEGPSRISLNCPISRTRIKTPVK 306

Query: 1168 GHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVDVL 989
            GHLCKH QCFDYD+++ INSR+P WRCP C++ V C+DIRIDQN+VKVLREV E   DV+
Sbjct: 307  GHLCKHHQCFDYDNFMEINSRRPSWRCPHCNQTVCCTDIRIDQNMVKVLREVAEGVADVI 366

Query: 988  VSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSDPSRRNQS 860
            ++ DGSWK V EN D    + + T   QE+   +S P++ ++S
Sbjct: 367  IAADGSWKPVFENDDSVHQTQNTT--CQEDGSEQSIPTKFSKS 407


>XP_010247436.1 PREDICTED: E4 SUMO-protein ligase PIAL1-like isoform X1 [Nelumbo
            nucifera]
          Length = 947

 Score =  176 bits (446), Expect = 3e-44
 Identities = 85/163 (52%), Positives = 115/163 (70%), Gaps = 1/163 (0%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVAS-GGPGIEMALQTSLNCPLSKSRMRTPVK 1169
            N  YII +  M  + +S  P+LQDYVQP V+S     IE   + SLNCP+S++R++TPVK
Sbjct: 269  NAKYIIAVAFMSDVASSDNPELQDYVQPAVSSLDSEVIEGPSRISLNCPISRTRIKTPVK 328

Query: 1168 GHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVDVL 989
            GHLCKH QCFDYD+++ INSR+P WRCP C++ V C+DIRIDQN+VKVLREV E   DV+
Sbjct: 329  GHLCKHHQCFDYDNFMEINSRRPSWRCPHCNQTVCCTDIRIDQNMVKVLREVAEGVADVI 388

Query: 988  VSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSDPSRRNQS 860
            ++ DGSWK V EN D    + + T   QE+   +S P++ ++S
Sbjct: 389  IAADGSWKPVFENDDSVHQTQNTT--CQEDGSEQSIPTKFSKS 429


>CDP12081.1 unnamed protein product [Coffea canephora]
          Length = 929

 Score =  174 bits (441), Expect = 1e-43
 Identities = 115/324 (35%), Positives = 166/324 (51%), Gaps = 21/324 (6%)
 Frame = -3

Query: 1345 NGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPG---IEMALQTSLNCPLSKSRMRTP 1175
            NG YI+VI +M  I     P L DYVQP  A   P    IE   + SLNCP+S   +RTP
Sbjct: 274  NGNYIVVIALMAEISKGENPTLPDYVQPAAAIIDPDSEVIEGPSRISLNCPISFRHIRTP 333

Query: 1174 VKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGENTVD 995
            VKGH CKH QCFD+D+Y+ INS++P WRCP C+     +DIRIDQN+VKVL+EVG+N  D
Sbjct: 334  VKGHTCKHLQCFDFDNYVDINSKRPSWRCPHCNHHCCFTDIRIDQNMVKVLKEVGDNVND 393

Query: 994  VLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSD--------PSRRNQSEADNC-- 845
            V++S DGSWK + E+ D       K P  ++E+  + D        P   + +E D+   
Sbjct: 394  VIISSDGSWKAIVESDDHAEKRQDKFPSAEQEQPTQPDSTSLPNAPPDLLDLTEIDDVMD 453

Query: 844  SQQISPTKNIQPSRVNSMHQNTCSSNSLA-ADCTNLNDQGNNNPRSMNVKPNGLSAAMST 668
            +  +S  ++ +   VNS  Q  CS   +     TN+ ++   N  S           +ST
Sbjct: 454  TVDLSEAEDTKVFLVNS--QKDCSIKDMTLRPPTNITNEVPQNSSSQTEDDFWSGVYLST 511

Query: 667  INDCKGCQLIQKDADLRNQTSGACINTSSS-------ANSLSPLQNLVSQNSNAYILPTV 509
                 G   +  DA    Q+ G   +TSSS        ++ SP  N+ ++ SNA++  +V
Sbjct: 512  FG--SGTFSLMSDA----QSGGVPQSTSSSILPSPLLTDASSPASNVEARASNAFLSNSV 565

Query: 508  EKNGCYQNSQGSYTYQNLNKSSNN 437
             +      +     +Q  N S +N
Sbjct: 566  PQTEISPTALQLQRFQFGNASISN 589



 Score = 64.7 bits (156), Expect = 2e-07
 Identities = 51/149 (34%), Positives = 73/149 (48%), Gaps = 15/149 (10%)
 Frame = -3

Query: 481  QGSYTYQNLNKSSNNICEAVRVQQVQLQRDGKVQAS----LAAQDRQPSVFVPCTRQPR- 317
            Q +  +Q+  +SS N   A + QQ   Q D  +QA+    L+AQ    +        PR 
Sbjct: 744  QSAGRFQHSAQSSGNFFRA-QSQQAGSQ-DHSIQAAHAQLLSAQRAAQAARTRAFHTPRA 801

Query: 316  ---------PEGERKQNMHEKKNQISHNDGLVELPSE-GWRPSGRMRGSLQGQAYSEAYR 167
                     P G++   +      +  +D  V  P++  WRPSGRMRGSL G+AYSEA  
Sbjct: 802  ASNSGNATAPVGDQIGAVGSTLQSVPRSDVSVNSPADQDWRPSGRMRGSLSGRAYSEAMN 861

Query: 166  QFIKQPTQHVRASELPPRLVATLSVAKSQ 80
            Q+I QPTQ  +A+  P  + A  S A +Q
Sbjct: 862  QYIIQPTQQAQAARPPSNVTANPSNASAQ 890


>CDP08753.1 unnamed protein product [Coffea canephora]
          Length = 667

 Score =  171 bits (433), Expect = 3e-43
 Identities = 138/453 (30%), Positives = 206/453 (45%), Gaps = 14/453 (3%)
 Frame = -3

Query: 1360 AIKISNGPYIIVIGVMCVIPTSGTPQLQDYVQPVVASGGPG--IEMALQTSLNCPLSKSR 1187
            AI    G Y++VI VM    +    +LQD V+P+  +      ++M+ + SLNCP+SKSR
Sbjct: 267  AIGQFKGNYLVVIAVMTTTQSFNLLELQD-VKPIALTNSETQTMQMSSRISLNCPISKSR 325

Query: 1186 MRTPVKGHLCKHTQCFDYDSYLSINSRKPYWRCPLCDEPVSCSDIRIDQNLVKVLREVGE 1007
            ++ PVKG LCKH QCFDYD++L INS++P W CP C +PV   DI ID N VKVL E  E
Sbjct: 326  IKMPVKGKLCKHFQCFDYDNFLEINSKRPSWECPHCKQPVCYVDICIDPNFVKVLSEAEE 385

Query: 1006 NTVDVLVSKDGSWKVVSENIDPPSPSASKTPGFQEEKQNRSDPSRRNQSEADNCSQQISP 827
               +V++S +G+WK   E+ D PS  +  +   Q+  ++RS   R   +   N ++ +S 
Sbjct: 386  KVGNVIMSANGAWK---EDKDHPSQMSGDSQVGQQNDESRSTTDR--STSLQNYNEIVSA 440

Query: 826  TKNIQPSRVNSMHQNTCSSNSLAADCTNLNDQGNNNPRSMNVKPNGLSAAMSTINDCKGC 647
                 P++             L   C+  N +G  N  S +                   
Sbjct: 441  AGTSVPTQ----------KPPLNVLCSAFNIKGPVNNGSHH----------------NSV 474

Query: 646  QLIQKDADLRNQTSGACINTSSSANSLSPLQNLVSQNSNAYILPTVEKNGCYQNSQGSYT 467
            QL+QKD     Q+SGA I+++  +      Q+    N N                    T
Sbjct: 475  QLLQKDGPPHAQSSGANISSTRLSFKQQIQQHWSLSNLN--------------------T 514

Query: 466  YQNLNKSSNNICEAVRVQQVQLQRDGKVQASLAAQD--RQPSVFVPCTRQPRPEGERKQN 293
             + L+ + + +C +V       +R   +Q S A     R P         PR        
Sbjct: 515  GEALSNTESVVCSSVPAPMEPGKRIATIQTSSAVHQAARLPKDAPFLFTTPRASSRMNSM 574

Query: 292  MHEKKNQIS----HNDGLVEL-----PSEGWRPSGRMRGSLQGQAYSEAYRQFIKQPTQH 140
            M +    +      ND L  L      S  WRP+ RMRG L+G+AYS A +QF  QPT  
Sbjct: 575  MVDSTVVVEARRIRNDELQSLDAADGESAHWRPTNRMRGGLRGEAYSAALQQFGIQPTLR 634

Query: 139  VRASELPPRLVATLS-VAKSQSPNIKASSQTKT 44
             +AS  P   V+    +  + +    ++SQTK+
Sbjct: 635  AQASRPPSSAVSIRGPLTNAVADRSASASQTKS 667