BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Angelica23_contig00005311
         (2161 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast prec...   627   0.0  
ref|XP_002304493.1| predicted protein [Populus trichocarpa] gi|2...   630   0.0  
gb|ABK96626.1| unknown [Populus trichocarpa x Populus deltoides]      628   0.0  
sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase,...   617   0.0  
gb|AFP93564.1| GCS [Cestrum nocturnum]                                617   0.0  

>ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast precursor, putative [Ricinus
            communis] gi|223549699|gb|EEF51187.1| Glutamate--cysteine
            ligase, chloroplast precursor, putative [Ricinus
            communis]
          Length = 526

 Score =  627 bits (1618), Expect(3) = 0.0
 Identities = 307/374 (82%), Positives = 329/374 (87%), Gaps = 1/374 (0%)
 Frame = -3

Query: 2135 GSSYCIPSESMQCKAGQN-VYSIANIVEASRIKGSCVSFASLTCDQRNTLQNLHSEGIRS 1959
            G S  +P++  +CK GQN V  + N +E S++K +CV F+SL+C+     Q   SE    
Sbjct: 8    GPSCYVPTDITRCKTGQNAVLGVFNSIETSKLKETCVRFSSLSCNSTKIPQLPSSENSWV 67

Query: 1958 GRKRGKQVIVAASPPTEDAVIATEPLTKEDLVAYLASGCKPKENWRIGTEHEKFGFESET 1779
              +RG Q+IVAASPP EDAVIATEPLTKEDLV YLASGCKPKE WRIGTEHEKFGFE  T
Sbjct: 68   RSRRGNQMIVAASPPIEDAVIATEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFELGT 127

Query: 1778 LRPMKYEQISELLYGISERFDWDKIMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLH 1599
            LRPMKYEQI+ELL GI+ERFDW+K+MEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLH
Sbjct: 128  LRPMKYEQIAELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLH 187

Query: 1598 QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWERKDIPIMPKGRYEIMRNYMPKVGTLG 1419
            QTCAEVNSHLYQVKAV EEMGIGFLGIGFQPKW  KDIPIMPKGRYEIMRNYMPKVGTLG
Sbjct: 188  QTCAEVNSHLYQVKAVTEEMGIGFLGIGFQPKWGVKDIPIMPKGRYEIMRNYMPKVGTLG 247

Query: 1418 LDMMFRTCTVQVNLDFSSEADMVRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQI 1239
            LDMMFRTCTVQVNLDFSSEADM+RKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQI
Sbjct: 248  LDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQI 307

Query: 1238 WTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDFLAGKLS 1059
            WTDTD +R GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDC G++FRDFLAGKL 
Sbjct: 308  WTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLP 367

Query: 1058 PIPGEYPNLNDWEN 1017
             IPGE P LNDWEN
Sbjct: 368  CIPGELPTLNDWEN 381



 Score =  143 bits (361), Expect(3) = 0.0
 Identities = 67/79 (84%), Positives = 75/79 (94%)
 Frame = -1

Query: 676 VPVTGLKTPFRDGLLRHVAQDVMQLAKDGLERRGYKETGFLNEVAEVARTGVTPAEKLLE 497
           VP TGLKTPFRDGLL+HVA+DV++LAKDGLERRGYKE GFLNEV EV RTGVTPAE+LLE
Sbjct: 448 VPKTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEIGFLNEVTEVVRTGVTPAERLLE 507

Query: 496 LYHGKWGKSVDPVFQELLY 440
           LY+GKWG+SVDPVF+ELLY
Sbjct: 508 LYNGKWGQSVDPVFEELLY 526



 Score =  117 bits (292), Expect(3) = 0.0
 Identities = 53/58 (91%), Positives = 55/58 (94%)
 Frame = -2

Query: 912 VRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQKVLDMVADWTMEEREMLRNK 739
           VRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDE+SLQ VLDM ADWT EEREMLRNK
Sbjct: 390 VRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEISLQNVLDMTADWTPEEREMLRNK 447


>ref|XP_002304493.1| predicted protein [Populus trichocarpa] gi|222841925|gb|EEE79472.1|
            predicted protein [Populus trichocarpa]
          Length = 526

 Score =  630 bits (1625), Expect(3) = 0.0
 Identities = 305/374 (81%), Positives = 335/374 (89%), Gaps = 1/374 (0%)
 Frame = -3

Query: 2135 GSSYCIPSESMQCKAGQNVY-SIANIVEASRIKGSCVSFASLTCDQRNTLQNLHSEGIRS 1959
            G SYCIP++  +CK  QNV   +A+ ++AS++K +   F+SL+C+   T + L SE +  
Sbjct: 8    GPSYCIPTDISRCKTAQNVVIGMASNIDASKLKETPARFSSLSCNSSKTPKILSSECLSV 67

Query: 1958 GRKRGKQVIVAASPPTEDAVIATEPLTKEDLVAYLASGCKPKENWRIGTEHEKFGFESET 1779
            G KRG Q +VAASPPTEDAV+ATEPLTKEDL+ YLASGCKPKENWRIGTEHEKFGFE  T
Sbjct: 68   GMKRGHQFVVAASPPTEDAVVATEPLTKEDLIRYLASGCKPKENWRIGTEHEKFGFEIGT 127

Query: 1778 LRPMKYEQISELLYGISERFDWDKIMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLH 1599
            LRPMKYEQI+ELL+GI+ERFDWDKIMEGD IIGLKQ KQSISLEPGGQFELSGAP+ETLH
Sbjct: 128  LRPMKYEQIAELLHGIAERFDWDKIMEGDTIIGLKQEKQSISLEPGGQFELSGAPVETLH 187

Query: 1598 QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWERKDIPIMPKGRYEIMRNYMPKVGTLG 1419
            QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKW  KDIPIMPKGRY+IMRNYMPKVG+LG
Sbjct: 188  QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWVLKDIPIMPKGRYDIMRNYMPKVGSLG 247

Query: 1418 LDMMFRTCTVQVNLDFSSEADMVRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQI 1239
            LDMMFRTCTVQVNLDFSSEADM+RKFRAGLALQPIATALFANSPFT+GKPNGYLS RS I
Sbjct: 248  LDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTDGKPNGYLSKRSHI 307

Query: 1238 WTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDFLAGKLS 1059
            WTDTDNNRAGMLPFVFDD+FGFEQYVDYALDVPMYFVYRK+KYIDC G+SFRDFLAG+L 
Sbjct: 308  WTDTDNNRAGMLPFVFDDNFGFEQYVDYALDVPMYFVYRKEKYIDCTGMSFRDFLAGRLP 367

Query: 1058 PIPGEYPNLNDWEN 1017
             IPGE P LNDWEN
Sbjct: 368  CIPGELPTLNDWEN 381



 Score =  143 bits (361), Expect(3) = 0.0
 Identities = 66/79 (83%), Positives = 75/79 (94%)
 Frame = -1

Query: 676 VPVTGLKTPFRDGLLRHVAQDVMQLAKDGLERRGYKETGFLNEVAEVARTGVTPAEKLLE 497
           VP TGLKTPFRDGLLRHVA++V++LAKDGLERRG+KE GFLN VAEVA TG+TPAEKLLE
Sbjct: 448 VPKTGLKTPFRDGLLRHVAEEVLKLAKDGLERRGFKEVGFLNAVAEVASTGITPAEKLLE 507

Query: 496 LYHGKWGKSVDPVFQELLY 440
           LYHGKWG+S+DPVF+ELLY
Sbjct: 508 LYHGKWGQSIDPVFEELLY 526



 Score =  114 bits (284), Expect(3) = 0.0
 Identities = 52/58 (89%), Positives = 54/58 (93%)
 Frame = -2

Query: 912 VRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQKVLDMVADWTMEEREMLRNK 739
           VRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDE SLQ VLDM ADWT EER+MLRNK
Sbjct: 390 VRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQSVLDMTADWTSEERQMLRNK 447


>gb|ABK96626.1| unknown [Populus trichocarpa x Populus deltoides]
          Length = 526

 Score =  628 bits (1619), Expect(3) = 0.0
 Identities = 304/374 (81%), Positives = 334/374 (89%), Gaps = 1/374 (0%)
 Frame = -3

Query: 2135 GSSYCIPSESMQCKAGQNVY-SIANIVEASRIKGSCVSFASLTCDQRNTLQNLHSEGIRS 1959
            G SYCIP++  +CK  QNV   +A+ ++AS++K +   F+SL+C+   T + L SE +  
Sbjct: 8    GPSYCIPTDISRCKTAQNVVIGMASNIDASKLKETPARFSSLSCNSSKTPKILSSECLSV 67

Query: 1958 GRKRGKQVIVAASPPTEDAVIATEPLTKEDLVAYLASGCKPKENWRIGTEHEKFGFESET 1779
            G KRG Q +VAASPPTEDAV+ATEPLTKEDL+ YLASGCKPKENWRIGTEHEKFGFE  T
Sbjct: 68   GMKRGHQFVVAASPPTEDAVVATEPLTKEDLIRYLASGCKPKENWRIGTEHEKFGFEIGT 127

Query: 1778 LRPMKYEQISELLYGISERFDWDKIMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLH 1599
            LRPMKYEQI+ELL+GI+ERFDWDKIMEGD IIGLKQ KQSISLEPGGQFELSGAP+ETLH
Sbjct: 128  LRPMKYEQIAELLHGIAERFDWDKIMEGDTIIGLKQEKQSISLEPGGQFELSGAPVETLH 187

Query: 1598 QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWERKDIPIMPKGRYEIMRNYMPKVGTLG 1419
            QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKW  KDIPIMPKGRY+IMRNYMPKVG+LG
Sbjct: 188  QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWVLKDIPIMPKGRYDIMRNYMPKVGSLG 247

Query: 1418 LDMMFRTCTVQVNLDFSSEADMVRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQI 1239
            LDMMFRTCTVQVNLDFSSEADM+RKFRAGLALQPIATALFANSPFT+GKPNGYLS RS I
Sbjct: 248  LDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTDGKPNGYLSKRSHI 307

Query: 1238 WTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDFLAGKLS 1059
            WTDTDNNRAG LPFVFDD+FGFEQYVDYALDVPMYFVYRK+KYIDC G+SFRDFLAG+L 
Sbjct: 308  WTDTDNNRAGKLPFVFDDNFGFEQYVDYALDVPMYFVYRKEKYIDCTGMSFRDFLAGRLP 367

Query: 1058 PIPGEYPNLNDWEN 1017
             IPGE P LNDWEN
Sbjct: 368  CIPGELPTLNDWEN 381



 Score =  142 bits (357), Expect(3) = 0.0
 Identities = 65/79 (82%), Positives = 74/79 (93%)
 Frame = -1

Query: 676 VPVTGLKTPFRDGLLRHVAQDVMQLAKDGLERRGYKETGFLNEVAEVARTGVTPAEKLLE 497
           VP TGLKTPFRDGLLRHVA++V++LAKDGLERRG+KE GFLN VAEVA TG+TPAEKLLE
Sbjct: 448 VPKTGLKTPFRDGLLRHVAEEVLKLAKDGLERRGFKEVGFLNAVAEVASTGITPAEKLLE 507

Query: 496 LYHGKWGKSVDPVFQELLY 440
           LYHGKWG+S+DP F+ELLY
Sbjct: 508 LYHGKWGQSIDPAFEELLY 526



 Score =  114 bits (284), Expect(3) = 0.0
 Identities = 52/58 (89%), Positives = 54/58 (93%)
 Frame = -2

Query: 912 VRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQKVLDMVADWTMEEREMLRNK 739
           VRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDE SLQ VLDM ADWT EER+MLRNK
Sbjct: 390 VRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEDSLQSVLDMTADWTSEERQMLRNK 447


>sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase, chloroplastic; AltName:
            Full=Gamma-ECS; Short=GCS; AltName:
            Full=Gamma-glutamylcysteine synthetase; Flags: Precursor
            gi|111380512|gb|ABD98695.2| chloroplast
            gamma-glutamylcysteine synthetase [Nicotiana tabacum]
          Length = 522

 Score =  617 bits (1592), Expect(3) = 0.0
 Identities = 305/380 (80%), Positives = 334/380 (87%), Gaps = 1/380 (0%)
 Frame = -3

Query: 2153 MATLSM-GSSYCIPSESMQCKAGQNVYSIANIVEASRIKGSCVSFASLTCDQRNTLQNLH 1977
            MA +S  GSS+CI SE M+C +G +  SI + +E  ++K  C    S + +    +Q ++
Sbjct: 1    MALMSQAGSSHCIYSEKMKCISGHS--SITSNMEMLKMKDICFGNIS-SRNSSKPMQGIY 57

Query: 1976 SEGIRSGRKRGKQVIVAASPPTEDAVIATEPLTKEDLVAYLASGCKPKENWRIGTEHEKF 1797
             + +   R+RG+  IVAASPPTEDAV+A EPLTKEDLVAYLASGCK KE WRIGTEHEKF
Sbjct: 58   LDRVGVERRRGRLAIVAASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKF 117

Query: 1796 GFESETLRPMKYEQISELLYGISERFDWDKIMEGDYIIGLKQGKQSISLEPGGQFELSGA 1617
            GFE  TLRPMKYEQI+ELL GI+ERFDW+K+MEGD IIGLKQGKQSISLEPGGQFELSGA
Sbjct: 118  GFEFGTLRPMKYEQIAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGA 177

Query: 1616 PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWERKDIPIMPKGRYEIMRNYMP 1437
            PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLG GFQPKW  KDIP+MPKGRYEIMRNYMP
Sbjct: 178  PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPVMPKGRYEIMRNYMP 237

Query: 1436 KVGTLGLDMMFRTCTVQVNLDFSSEADMVRKFRAGLALQPIATALFANSPFTEGKPNGYL 1257
            KVG+LGLDMMFRTCTVQVNLDFSSEADM+RKFRAGLALQPIATALFANSPFTEGKPNGYL
Sbjct: 238  KVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYL 297

Query: 1256 SMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDF 1077
            SMRS IWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAG+SFRDF
Sbjct: 298  SMRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDF 357

Query: 1076 LAGKLSPIPGEYPNLNDWEN 1017
            + GKLSPIPG+YP LNDWEN
Sbjct: 358  MNGKLSPIPGDYPTLNDWEN 377



 Score =  148 bits (374), Expect(3) = 0.0
 Identities = 69/79 (87%), Positives = 76/79 (96%)
 Frame = -1

Query: 676 VPVTGLKTPFRDGLLRHVAQDVMQLAKDGLERRGYKETGFLNEVAEVARTGVTPAEKLLE 497
           VP +GLKTPFRDGLL+HVAQDV++LAK+GLERRGYKETGFLNEV EV RTGVTPAEKLLE
Sbjct: 444 VPTSGLKTPFRDGLLKHVAQDVVKLAKEGLERRGYKETGFLNEVTEVVRTGVTPAEKLLE 503

Query: 496 LYHGKWGKSVDPVFQELLY 440
           LYHGKWG+SVDPVF+ELLY
Sbjct: 504 LYHGKWGRSVDPVFEELLY 522



 Score =  117 bits (292), Expect(3) = 0.0
 Identities = 54/58 (93%), Positives = 55/58 (94%)
 Frame = -2

Query: 912 VRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQKVLDMVADWTMEEREMLRNK 739
           VRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQ VLDM +DWT EEREMLRNK
Sbjct: 386 VRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQTVLDMTSDWTAEEREMLRNK 443


>gb|AFP93564.1| GCS [Cestrum nocturnum]
          Length = 518

 Score =  617 bits (1592), Expect(3) = 0.0
 Identities = 304/380 (80%), Positives = 327/380 (86%), Gaps = 1/380 (0%)
 Frame = -3

Query: 2153 MATLSM-GSSYCIPSESMQCKAGQNVYSIANIVEASRIKGSCVSFASLTCDQRNTLQNLH 1977
            MA +S  GSS+CI  E ++CK G N+       E  ++K  C        +  N  Q + 
Sbjct: 1    MALMSQAGSSHCIHPEKIRCKGGHNM-------EIFKLKEICFGVHISPRNSSNAKQGIC 53

Query: 1976 SEGIRSGRKRGKQVIVAASPPTEDAVIATEPLTKEDLVAYLASGCKPKENWRIGTEHEKF 1797
               + + R+RG+  IVAASPPT+DAV+A EPLTKEDLVAYLASGCK KE WRIGTEHEKF
Sbjct: 54   LRRVGAERRRGRLSIVAASPPTDDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKF 113

Query: 1796 GFESETLRPMKYEQISELLYGISERFDWDKIMEGDYIIGLKQGKQSISLEPGGQFELSGA 1617
            GFE  TLRPM YEQI+ELLYG+SERFDW+K+MEGD IIGLKQGKQSISLEPGGQFELSGA
Sbjct: 114  GFEFGTLRPMNYEQIAELLYGVSERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGA 173

Query: 1616 PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWERKDIPIMPKGRYEIMRNYMP 1437
            PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKW  KDIP+MPKGRYEIMRNYMP
Sbjct: 174  PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMP 233

Query: 1436 KVGTLGLDMMFRTCTVQVNLDFSSEADMVRKFRAGLALQPIATALFANSPFTEGKPNGYL 1257
            KVGTLGLDMMFRTCTVQVNLDFSSEADM+RKFRAGLALQPIATALFANSPF EGKPNGYL
Sbjct: 234  KVGTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFKEGKPNGYL 293

Query: 1256 SMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDF 1077
            SMRS IWTDTDNNR+GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDF
Sbjct: 294  SMRSHIWTDTDNNRSGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDF 353

Query: 1076 LAGKLSPIPGEYPNLNDWEN 1017
            L GKL+PIPGEYP LNDWEN
Sbjct: 354  LNGKLAPIPGEYPTLNDWEN 373



 Score =  147 bits (372), Expect(3) = 0.0
 Identities = 69/79 (87%), Positives = 76/79 (96%)
 Frame = -1

Query: 676 VPVTGLKTPFRDGLLRHVAQDVMQLAKDGLERRGYKETGFLNEVAEVARTGVTPAEKLLE 497
           VP +GLKTPFRDGLL+HVAQDVM+LAK+GLERRGYKETGFLNEV EV RTGVTPAEKLL+
Sbjct: 440 VPKSGLKTPFRDGLLKHVAQDVMKLAKEGLERRGYKETGFLNEVTEVVRTGVTPAEKLLD 499

Query: 496 LYHGKWGKSVDPVFQELLY 440
           LYHGKWG+SVDPVF+ELLY
Sbjct: 500 LYHGKWGQSVDPVFEELLY 518



 Score =  117 bits (292), Expect(3) = 0.0
 Identities = 54/58 (93%), Positives = 54/58 (93%)
 Frame = -2

Query: 912 VRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQKVLDMVADWTMEEREMLRNK 739
           VRLKRYLEMRGADGGPWRRLCALPAFWVGILYDE SLQ VLDM ADWT EEREMLRNK
Sbjct: 382 VRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEASLQSVLDMTADWTAEEREMLRNK 439