BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Angelica22_contig00042593
         (447 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003635448.1| PREDICTED: LRR receptor-like serine/threonin...   177   6e-43
emb|CAN78527.1| hypothetical protein VITISV_039533 [Vitis vinifera]   175   4e-42
emb|CAN72496.1| hypothetical protein VITISV_010511 [Vitis vinifera]   173   1e-41
ref|XP_003633000.1| PREDICTED: LRR receptor-like serine/threonin...   173   2e-41
ref|XP_004150225.1| PREDICTED: probable LRR receptor-like serine...   171   4e-41

>ref|XP_003635448.1| PREDICTED: LRR receptor-like serine/threonine-protein kinase
            GSO1-like [Vitis vinifera]
          Length = 1140

 Score =  177 bits (450), Expect = 6e-43
 Identities = 90/147 (61%), Positives = 111/147 (75%)
 Frame = +2

Query: 2    CQLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLS 181
            C LK+LG L+LS NKL+GSIP C GDL +LQ L+LDSN L   IP+SLWSL DL+ LNLS
Sbjct: 594  CHLKDLGYLFLSSNKLSGSIPSCFGDLLALQELFLDSNVLAFNIPTSLWSLRDLLALNLS 653

Query: 182  SNMITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTL 361
            SN +TG++P E+ N+K IT + LS N +SGY+PS +G  Q LI LS+S N LQGP+P   
Sbjct: 654  SNFLTGNLPPEVGNMKSITTLDLSKNLVSGYIPSKMGKLQSLITLSLSQNRLQGPIPIEF 713

Query: 362  GNLINLEYLDLSSNRFSGAIPKSLEAL 442
            G+L++LE LDLS N  SG IPKSLEAL
Sbjct: 714  GDLVSLESLDLSQNNLSGTIPKSLEAL 740



 Score = 97.1 bits (240), Expect = 1e-18
 Identities = 56/137 (40%), Positives = 81/137 (59%), Gaps = 1/137 (0%)
 Frame = +2

Query: 26  LYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSI 205
           L LS NK  GSIP  +G+L  L+ +YL +N L   IP+S  +L  L  LNL  N +TG++
Sbjct: 329 LSLSFNKFRGSIPKEIGNLSKLEEIYLGTNSLIGSIPTSFGNLKALKFLNLGINNLTGTV 388

Query: 206 PMEIQNLKQITEIGLSWNQLSGYLPSTIG-GAQMLIYLSVSHNELQGPLPQTLGNLINLE 382
           P  I N+ ++  + +  N LSG LPS+IG     L  L ++ NE  G +P ++ N+  L 
Sbjct: 389 PEAIFNISKLQSLAMVKNHLSGSLPSSIGTWLPDLEGLFIAGNEFSGIIPMSISNMSKLT 448

Query: 383 YLDLSSNRFSGAIPKSL 433
            L LS+N F+G +PK L
Sbjct: 449 VLGLSANSFTGNVPKDL 465



 Score = 95.9 bits (237), Expect = 3e-18
 Identities = 47/138 (34%), Positives = 87/138 (63%), Gaps = 1/138 (0%)
 Frame = +2

Query: 2   CQLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLS 181
           C L  L +LYL +N+L G IP  +  L++L+ L    N L   IP+++++++ L+ ++LS
Sbjct: 120 CNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNNLTGSIPATIFNISSLLNISLS 179

Query: 182 SNMITGSIPMEIQNLK-QITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQT 358
           +N ++GS+PM++     ++ E+ LS N LSG +P+ +G    L  +S+++N+  G +P  
Sbjct: 180 NNNLSGSLPMDMCYANPKLKELNLSSNHLSGKIPTGLGQCIQLQVISLAYNDFTGSIPSG 239

Query: 359 LGNLINLEYLDLSSNRFS 412
           + NL+ L+ L L +N F+
Sbjct: 240 IDNLVELQRLSLQNNSFT 257



 Score = 95.1 bits (235), Expect = 5e-18
 Identities = 59/145 (40%), Positives = 81/145 (55%), Gaps = 1/145 (0%)
 Frame = +2

Query: 11  KNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNM 190
           K L  L L +NKL G IP  + +L  L+ LYL +NQL   IP  +  L +L  L+   N 
Sbjct: 99  KELQQLNLFNNKLVGGIPEAICNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNN 158

Query: 191 ITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQ-MLIYLSVSHNELQGPLPQTLGN 367
           +TGSIP  I N+  +  I LS N LSG LP  +  A   L  L++S N L G +P  LG 
Sbjct: 159 LTGSIPATIFNISSLLNISLSNNNLSGSLPMDMCYANPKLKELNLSSNHLSGKIPTGLGQ 218

Query: 368 LINLEYLDLSSNRFSGAIPKSLEAL 442
            I L+ + L+ N F+G+IP  ++ L
Sbjct: 219 CIQLQVISLAYNDFTGSIPSGIDNL 243



 Score = 87.4 bits (215), Expect = 1e-15
 Identities = 53/143 (37%), Positives = 82/143 (57%), Gaps = 1/143 (0%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L  L  L LSDN  +GS+P  +G  + LQ+L L +N+L   IP ++ +L+ L  L L +N
Sbjct: 74  LSFLVSLDLSDNYFHGSLPKDIGKCKELQQLNLFNNKLVGGIPEAICNLSKLEELYLGNN 133

Query: 188 MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
            + G IP ++ +L+ +  +    N L+G +P+TI     L+ +S+S+N L G LP  +  
Sbjct: 134 QLIGEIPKKMNHLQNLKVLSFPMNNLTGSIPATIFNISSLLNISLSNNNLSGSLPMDMCY 193

Query: 368 L-INLEYLDLSSNRFSGAIPKSL 433
               L+ L+LSSN  SG IP  L
Sbjct: 194 ANPKLKELNLSSNHLSGKIPTGL 216



 Score = 85.5 bits (210), Expect = 4e-15
 Identities = 46/136 (33%), Positives = 77/136 (56%), Gaps = 1/136 (0%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L  L ++YL  N L GSIP   G+L++L+ L L  N L   +P ++++++ L  L +  N
Sbjct: 347 LSKLEEIYLGTNSLIGSIPTSFGNLKALKFLNLGINNLTGTVPEAIFNISKLQSLAMVKN 406

Query: 188 MITGSIPMEIQN-LKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLG 364
            ++GS+P  I   L  +  + ++ N+ SG +P +I     L  L +S N   G +P+ LG
Sbjct: 407 HLSGSLPSSIGTWLPDLEGLFIAGNEFSGIIPMSISNMSKLTVLGLSANSFTGNVPKDLG 466

Query: 365 NLINLEYLDLSSNRFS 412
           NL  L+ LDL+ N+ +
Sbjct: 467 NLTKLKVLDLAGNQLT 482



 Score = 82.8 bits (203), Expect = 3e-14
 Identities = 48/141 (34%), Positives = 76/141 (53%)
 Frame = +2

Query: 11  KNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNM 190
           +++  + LS+  L G+I P +G+L  L  L L  N  +  +P  +    +L  LNL +N 
Sbjct: 51  QSVSAINLSNMGLEGTIAPQVGNLSFLVSLDLSDNYFHGSLPKDIGKCKELQQLNLFNNK 110

Query: 191 ITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNL 370
           + G IP  I NL ++ E+ L  NQL G +P  +   Q L  LS   N L G +P T+ N+
Sbjct: 111 LVGGIPEAICNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNNLTGSIPATIFNI 170

Query: 371 INLEYLDLSSNRFSGAIPKSL 433
            +L  + LS+N  SG++P  +
Sbjct: 171 SSLLNISLSNNNLSGSLPMDM 191



 Score = 82.8 bits (203), Expect = 3e-14
 Identities = 49/146 (33%), Positives = 87/146 (59%), Gaps = 1/146 (0%)
 Frame = +2

Query: 11  KNLGDLYLSDNKLNGSIPPCLGDLE-SLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           K L +L++ +    G++P  LG+L  +L+     + Q    IP+ + +LT+LI L+L +N
Sbjct: 500 KFLKNLWIGNIPFKGTLPNSLGNLPIALESFIASACQFRGTIPTGIGNLTNLIRLDLGAN 559

Query: 188 MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
            +TGSIP  +  L+++  + ++ N++ G +P+ +   + L YL +S N+L G +P   G+
Sbjct: 560 DLTGSIPTTLGQLQKLQWLYIAGNRIRGSIPNDLCHLKDLGYLFLSSNKLSGSIPSCFGD 619

Query: 368 LINLEYLDLSSNRFSGAIPKSLEALR 445
           L+ L+ L L SN  +  IP SL +LR
Sbjct: 620 LLALQELFLDSNVLAFNIPTSLWSLR 645



 Score = 82.4 bits (202), Expect = 3e-14
 Identities = 48/146 (32%), Positives = 85/146 (58%), Gaps = 8/146 (5%)
 Frame = +2

Query: 32  LSDNKLNGSIPPCLGDLESLQRLYLDSNQLNS-------IIPSSLWSLTDLIGLNLSSNM 190
           L+ N   GSIP  + +L  LQRL L +N   +       ++ + +++++ L  +  + N 
Sbjct: 227 LAYNDFTGSIPSGIDNLVELQRLSLQNNSFTAFKDISKALLFAEIFNVSSLQVIAFTDNS 286

Query: 191 ITGSIPMEI-QNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
           ++GS+P +I ++L  +  + LS N LSG LP+T+     L++LS+S N+ +G +P+ +GN
Sbjct: 287 LSGSLPKDICKHLPNLQGLSLSQNHLSGQLPTTLSLCGELLFLSLSFNKFRGSIPKEIGN 346

Query: 368 LINLEYLDLSSNRFSGAIPKSLEALR 445
           L  LE + L +N   G+IP S   L+
Sbjct: 347 LSKLEEIYLGTNSLIGSIPTSFGNLK 372



 Score = 68.6 bits (166), Expect = 5e-10
 Identities = 58/176 (32%), Positives = 81/176 (46%), Gaps = 34/176 (19%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPP--CLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLS 181
           + +L ++ LS+N L+GS+P   C  +   L+ L L SN L+  IP+ L     L  ++L+
Sbjct: 170 ISSLLNISLSNNNLSGSLPMDMCYAN-PKLKELNLSSNHLSGKIPTGLGQCIQLQVISLA 228

Query: 182 SNMITGSIPMEIQNLKQITEIGL-------------------------------SWNQLS 268
            N  TGSIP  I NL ++  + L                               + N LS
Sbjct: 229 YNDFTGSIPSGIDNLVELQRLSLQNNSFTAFKDISKALLFAEIFNVSSLQVIAFTDNSLS 288

Query: 269 GYLPSTI-GGAQMLIYLSVSHNELQGPLPQTLGNLINLEYLDLSSNRFSGAIPKSL 433
           G LP  I      L  LS+S N L G LP TL     L +L LS N+F G+IPK +
Sbjct: 289 GSLPKDICKHLPNLQGLSLSQNHLSGQLPTTLSLCGELLFLSLSFNKFRGSIPKEI 344



 Score = 66.2 bits (160), Expect = 3e-09
 Identities = 36/122 (29%), Positives = 65/122 (53%)
 Frame = +2

Query: 68  CLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSIPMEIQNLKQITEIG 247
           C    +S+  + L +  L   I   + +L+ L+ L+LS N   GS+P +I   K++ ++ 
Sbjct: 46  CNAPQQSVSAINLSNMGLEGTIAPQVGNLSFLVSLDLSDNYFHGSLPKDIGKCKELQQLN 105

Query: 248 LSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNLINLEYLDLSSNRFSGAIPK 427
           L  N+L G +P  I     L  L + +N+L G +P+ + +L NL+ L    N  +G+IP 
Sbjct: 106 LFNNKLVGGIPEAICNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNNLTGSIPA 165

Query: 428 SL 433
           ++
Sbjct: 166 TI 167


>emb|CAN78527.1| hypothetical protein VITISV_039533 [Vitis vinifera]
          Length = 1229

 Score =  175 bits (443), Expect = 4e-42
 Identities = 89/147 (60%), Positives = 109/147 (74%)
 Frame = +2

Query: 2    CQLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLS 181
            C LKNLG L+LS NKL+GSIP C GDL +LQ L+LDSN L   IP+SLWSL DL+ LNLS
Sbjct: 683  CHLKNLGYLHLSSNKLSGSIPSCFGDLPALQELFLDSNVLAFNIPTSLWSLRDLLVLNLS 742

Query: 182  SNMITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTL 361
            SN +TG++P E+ N+K IT + LS N +SGY+P  +G  Q L  LS+S N LQGP+P   
Sbjct: 743  SNFLTGNLPPEVGNMKSITTLDLSKNLVSGYIPRRMGEQQNLAKLSLSQNRLQGPIPVEF 802

Query: 362  GNLINLEYLDLSSNRFSGAIPKSLEAL 442
            G+L++LE LDLS N  SG IPKSLEAL
Sbjct: 803  GDLVSLESLDLSQNNLSGTIPKSLEAL 829



 Score =  114 bits (285), Expect = 8e-24
 Identities = 59/142 (41%), Positives = 96/142 (67%), Gaps = 1/142 (0%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L +L +LYL  NKL G IP  +G+L +L  L L SN ++  IP+ +++++ L G+  S+N
Sbjct: 315 LSDLEELYLGYNKLTGGIPREIGNLSNLNILQLGSNGISGPIPAEIFNISSLQGIGFSNN 374

Query: 188 MITGSIPMEI-QNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLG 364
            ++GS+PM+I ++L  +  + L+ N LSG LP+T+   + L+ LS+S N+ +G +P+ +G
Sbjct: 375 SLSGSLPMDICKHLPNLQWLDLALNHLSGQLPTTLSLCRELLVLSLSFNKFRGSIPREIG 434

Query: 365 NLINLEYLDLSSNRFSGAIPKS 430
           NL  LE++DLSSN   G+IP S
Sbjct: 435 NLSKLEWIDLSSNSLVGSIPTS 456



 Score = 97.1 bits (240), Expect = 1e-18
 Identities = 54/143 (37%), Positives = 81/143 (56%)
 Frame = +2

Query: 5   QLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSS 184
           Q   L  + L+ N   GSIP  +G+L  LQRL L +N L   IP  L++++ L  LNL+ 
Sbjct: 218 QCLKLQVISLAYNDFTGSIPSGIGNLVELQRLSLQNNSLTGEIPQLLFNISSLRLLNLAV 277

Query: 185 NMITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLG 364
           N + G IP  + + +++  + LS N+ +G +P  IG    L  L + +N+L G +P+ +G
Sbjct: 278 NNLEGEIPSNLSHCRELRVLSLSINRFTGGIPQAIGSLSDLEELYLGYNKLTGGIPREIG 337

Query: 365 NLINLEYLDLSSNRFSGAIPKSL 433
           NL NL  L L SN  SG IP  +
Sbjct: 338 NLSNLNILQLGSNGISGPIPAEI 360



 Score = 93.2 bits (230), Expect = 2e-17
 Identities = 53/166 (31%), Positives = 92/166 (55%), Gaps = 25/166 (15%)
 Frame = +2

Query: 11  KNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQ------------------------ 118
           K L  L L +NKL G IP  + +L  L+ LYL +NQ                        
Sbjct: 99  KELQQLNLFNNKLVGGIPEAICNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNN 158

Query: 119 LNSIIPSSLWSLTDLIGLNLSSNMITGSIPMEIQNLK-QITEIGLSWNQLSGYLPSTIGG 295
           L   IP+++++++ L+ ++LS+N ++GS+PM++     ++ E+ LS N LSG +P+ +G 
Sbjct: 159 LTGFIPATIFNISSLLNISLSNNNLSGSLPMDMCYANPKLKELNLSSNHLSGKIPTGLGQ 218

Query: 296 AQMLIYLSVSHNELQGPLPQTLGNLINLEYLDLSSNRFSGAIPKSL 433
              L  +S+++N+  G +P  +GNL+ L+ L L +N  +G IP+ L
Sbjct: 219 CLKLQVISLAYNDFTGSIPSGIGNLVELQRLSLQNNSLTGEIPQLL 264



 Score = 93.2 bits (230), Expect = 2e-17
 Identities = 52/139 (37%), Positives = 78/139 (56%)
 Frame = +2

Query: 17  LGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMIT 196
           L +L LS N L+G IP  LG    LQ + L  N     IPS + +L +L  L+L +N +T
Sbjct: 198 LKELNLSSNHLSGKIPTGLGQCLKLQVISLAYNDFTGSIPSGIGNLVELQRLSLQNNSLT 257

Query: 197 GSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNLIN 376
           G IP  + N+  +  + L+ N L G +PS +   + L  LS+S N   G +PQ +G+L +
Sbjct: 258 GEIPQLLFNISSLRLLNLAVNNLEGEIPSNLSHCRELRVLSLSINRFTGGIPQAIGSLSD 317

Query: 377 LEYLDLSSNRFSGAIPKSL 433
           LE L L  N+ +G IP+ +
Sbjct: 318 LEELYLGYNKLTGGIPREI 336



 Score = 92.8 bits (229), Expect = 3e-17
 Identities = 56/137 (40%), Positives = 79/137 (57%), Gaps = 1/137 (0%)
 Frame = +2

Query: 26  LYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSI 205
           L LS NK  GSIP  +G+L  L+ + L SN L   IP+S  +L  L  LNL  N +TG++
Sbjct: 418 LSLSFNKFRGSIPREIGNLSKLEWIDLSSNSLVGSIPTSFGNLMALKFLNLGINNLTGTV 477

Query: 206 PMEIQNLKQITEIGLSWNQLSGYLPSTIG-GAQMLIYLSVSHNELQGPLPQTLGNLINLE 382
           P  I N+ ++  + ++ N LSG LPS+IG     L  L +  NE  G +P ++ N+  L 
Sbjct: 478 PEAIFNISKLQSLAMAINHLSGSLPSSIGTWLPDLEGLFIGGNEFSGIIPVSISNMSKLT 537

Query: 383 YLDLSSNRFSGAIPKSL 433
            LD+S N F G +PK L
Sbjct: 538 QLDVSRNSFIGNVPKDL 554



 Score = 91.7 bits (226), Expect = 6e-17
 Identities = 55/173 (31%), Positives = 87/173 (50%), Gaps = 25/173 (14%)
 Frame = +2

Query: 2   CQLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIG---- 169
           C L  L +LYL +N+L G IP  +  L++L+ L    N L   IP+++++++ L+     
Sbjct: 120 CNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNNLTGFIPATIFNISSLLNISLS 179

Query: 170 ---------------------LNLSSNMITGSIPMEIQNLKQITEIGLSWNQLSGYLPST 286
                                LNLSSN ++G IP  +    ++  I L++N  +G +PS 
Sbjct: 180 NNNLSGSLPMDMCYANPKLKELNLSSNHLSGKIPTGLGQCLKLQVISLAYNDFTGSIPSG 239

Query: 287 IGGAQMLIYLSVSHNELQGPLPQTLGNLINLEYLDLSSNRFSGAIPKSLEALR 445
           IG    L  LS+ +N L G +PQ L N+ +L  L+L+ N   G IP +L   R
Sbjct: 240 IGNLVELQRLSLQNNSLTGEIPQLLFNISSLRLLNLAVNNLEGEIPSNLSHCR 292



 Score = 88.2 bits (217), Expect = 6e-16
 Identities = 52/146 (35%), Positives = 87/146 (59%), Gaps = 1/146 (0%)
 Frame = +2

Query: 11   KNLGDLYLSDNKLNGSIPPCLGDLE-SLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
            K L +L++ +N   G++P  LG+L  +L+     + Q    IP+ + +LT+LI L+L +N
Sbjct: 589  KFLKNLWIGNNPFKGTLPNSLGNLPIALESFIASACQFRGTIPTGIGNLTNLIWLDLGAN 648

Query: 188  MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
             +TGSIP  +  LK++  + ++ N+L G +P+ +   + L YL +S N+L G +P   G+
Sbjct: 649  DLTGSIPTILGRLKKLQRLHIAGNRLRGSIPNDLCHLKNLGYLHLSSNKLSGSIPSCFGD 708

Query: 368  LINLEYLDLSSNRFSGAIPKSLEALR 445
            L  L+ L L SN  +  IP SL +LR
Sbjct: 709  LPALQELFLDSNVLAFNIPTSLWSLR 734



 Score = 86.3 bits (212), Expect = 2e-15
 Identities = 55/147 (37%), Positives = 78/147 (53%), Gaps = 1/147 (0%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           + +L  L L+ N L G IP  L     L+ L L  N+    IP ++ SL+DL  L L  N
Sbjct: 267 ISSLRLLNLAVNNLEGEIPSNLSHCRELRVLSLSINRFTGGIPQAIGSLSDLEELYLGYN 326

Query: 188 MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTL-G 364
            +TG IP EI NL  +  + L  N +SG +P+ I     L  +  S+N L G LP  +  
Sbjct: 327 KLTGGIPREIGNLSNLNILQLGSNGISGPIPAEIFNISSLQGIGFSNNSLSGSLPMDICK 386

Query: 365 NLINLEYLDLSSNRFSGAIPKSLEALR 445
           +L NL++LDL+ N  SG +P +L   R
Sbjct: 387 HLPNLQWLDLALNHLSGQLPTTLSLCR 413



 Score = 81.3 bits (199), Expect = 8e-14
 Identities = 48/134 (35%), Positives = 73/134 (54%)
 Frame = +2

Query: 32  LSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSIPM 211
           LS+  L G+I P +G+L  L  L L +N  +  +P  +    +L  LNL +N + G IP 
Sbjct: 58  LSNMGLEGTIAPQVGNLSFLISLDLSNNYFHDSLPKDIGKCKELQQLNLFNNKLVGGIPE 117

Query: 212 EIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNLINLEYLD 391
            I NL ++ E+ L  NQL G +P  +   Q L  LS   N L G +P T+ N+ +L  + 
Sbjct: 118 AICNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNNLTGFIPATIFNISSLLNIS 177

Query: 392 LSSNRFSGAIPKSL 433
           LS+N  SG++P  +
Sbjct: 178 LSNNNLSGSLPMDM 191



 Score = 80.5 bits (197), Expect = 1e-13
 Identities = 53/143 (37%), Positives = 75/143 (52%), Gaps = 1/143 (0%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L NL  L L+ N L+G +P  L     L  L L  N+    IP  + +L+ L  ++LSSN
Sbjct: 388 LPNLQWLDLALNHLSGQLPTTLSLCRELLVLSLSFNKFRGSIPREIGNLSKLEWIDLSSN 447

Query: 188 MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
            + GSIP    NL  +  + L  N L+G +P  I     L  L+++ N L G LP ++G 
Sbjct: 448 SLVGSIPTSFGNLMALKFLNLGINNLTGTVPEAIFNISKLQSLAMAINHLSGSLPSSIGT 507

Query: 368 -LINLEYLDLSSNRFSGAIPKSL 433
            L +LE L +  N FSG IP S+
Sbjct: 508 WLPDLEGLFIGGNEFSGIIPVSI 530


>emb|CAN72496.1| hypothetical protein VITISV_010511 [Vitis vinifera]
          Length = 1241

 Score =  173 bits (439), Expect = 1e-41
 Identities = 88/147 (59%), Positives = 108/147 (73%)
 Frame = +2

Query: 2    CQLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLS 181
            C LKNLG L+L  NKL+GSIP C GDL +LQ L+LDSN L   IP+SLWSL DL+ LNLS
Sbjct: 695  CHLKNLGYLHLXSNKLSGSIPSCFGDLPALQELFLDSNVLAFNIPTSLWSLRDLLVLNLS 754

Query: 182  SNMITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTL 361
            SN +TG++P E+ N+K IT + LS N +SGY+P  +G  Q L  LS+S N LQGP+P   
Sbjct: 755  SNFLTGNLPPEVGNMKSITTLDLSKNLVSGYIPRRMGEQQNLAKLSLSQNRLQGPIPXEF 814

Query: 362  GNLINLEYLDLSSNRFSGAIPKSLEAL 442
            G+L++LE LDLS N  SG IPKSLEAL
Sbjct: 815  GDLVSLESLDLSQNNLSGTIPKSLEAL 841



 Score =  109 bits (272), Expect = 3e-22
 Identities = 56/142 (39%), Positives = 88/142 (61%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L NL +LYL+ NKL G IP  +G+L  L  L L SN ++  IP+ +++++ L  ++ S+N
Sbjct: 231 LCNLEELYLAFNKLTGGIPREIGNLSKLNILQLSSNGISGPIPTEIFNISSLQEIDFSNN 290

Query: 188 MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
            +TG IP  + + +++  + LS+NQ +G +P  IG    L  L +S+N+L G +P+ +GN
Sbjct: 291 SLTGEIPSNLSHCRELRVLSLSFNQFTGGIPQAIGSLSNLEGLYLSYNKLTGGIPREIGN 350

Query: 368 LINLEYLDLSSNRFSGAIPKSL 433
           L NL  L L SN  SG IP  +
Sbjct: 351 LSNLNILQLGSNGISGPIPAEI 372



 Score =  106 bits (264), Expect = 2e-21
 Identities = 60/141 (42%), Positives = 85/141 (60%)
 Frame = +2

Query: 11  KNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNM 190
           + L  L LS N+  G IP  +G L +L+ LYL  N+L   IP  + +L+ L  L LSSN 
Sbjct: 208 RELRGLSLSFNQFTGGIPQAIGSLCNLEELYLAFNKLTGGIPREIGNLSKLNILQLSSNG 267

Query: 191 ITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNL 370
           I+G IP EI N+  + EI  S N L+G +PS +   + L  LS+S N+  G +PQ +G+L
Sbjct: 268 ISGPIPTEIFNISSLQEIDFSNNSLTGEIPSNLSHCRELRVLSLSFNQFTGGIPQAIGSL 327

Query: 371 INLEYLDLSSNRFSGAIPKSL 433
            NLE L LS N+ +G IP+ +
Sbjct: 328 SNLEGLYLSYNKLTGGIPREI 348



 Score =  100 bits (250), Expect = 1e-19
 Identities = 50/149 (33%), Positives = 91/149 (61%), Gaps = 1/149 (0%)
 Frame = +2

Query: 2   CQLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLS 181
           C L  L +LYL +N+L G IP  +  L++L+ L    N L   IP+++++++ L+ ++LS
Sbjct: 60  CNLSKLEELYLGNNELIGEIPKKMNHLQNLKVLSFPMNNLTGSIPATIFNISSLLNISLS 119

Query: 182 SNMITGSIPMEIQNLK-QITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQT 358
           +N ++GS+P ++     ++ E+ LS N LSG +P+ +G    L  +S+++N+  G +P  
Sbjct: 120 NNNLSGSLPKDMCYANPKLKELNLSSNHLSGKIPTGLGQCIQLQVISLAYNDFTGSIPNG 179

Query: 359 LGNLINLEYLDLSSNRFSGAIPKSLEALR 445
           +GNL+ L+ L L +N  +G IP +    R
Sbjct: 180 IGNLVELQRLSLRNNSLTGEIPSNFSHCR 208



 Score =  100 bits (248), Expect = 2e-19
 Identities = 55/138 (39%), Positives = 81/138 (58%)
 Frame = +2

Query: 32  LSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSIPM 211
           L+ N   GSIP  +G+L  LQRL L +N L   IPS+     +L GL+LS N  TG IP 
Sbjct: 167 LAYNDFTGSIPNGIGNLVELQRLSLRNNSLTGEIPSNFSHCRELRGLSLSFNQFTGGIPQ 226

Query: 212 EIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNLINLEYLD 391
            I +L  + E+ L++N+L+G +P  IG    L  L +S N + GP+P  + N+ +L+ +D
Sbjct: 227 AIGSLCNLEELYLAFNKLTGGIPREIGNLSKLNILQLSSNGISGPIPTEIFNISSLQEID 286

Query: 392 LSSNRFSGAIPKSLEALR 445
            S+N  +G IP +L   R
Sbjct: 287 FSNNSLTGEIPSNLSHCR 304



 Score = 95.1 bits (235), Expect = 5e-18
 Identities = 53/139 (38%), Positives = 78/139 (56%)
 Frame = +2

Query: 17  LGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMIT 196
           L +L LS N L+G IP  LG    LQ + L  N     IP+ + +L +L  L+L +N +T
Sbjct: 138 LKELNLSSNHLSGKIPTGLGQCIQLQVISLAYNDFTGSIPNGIGNLVELQRLSLRNNSLT 197

Query: 197 GSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNLIN 376
           G IP    + +++  + LS+NQ +G +P  IG    L  L ++ N+L G +P+ +GNL  
Sbjct: 198 GEIPSNFSHCRELRGLSLSFNQFTGGIPQAIGSLCNLEELYLAFNKLTGGIPREIGNLSK 257

Query: 377 LEYLDLSSNRFSGAIPKSL 433
           L  L LSSN  SG IP  +
Sbjct: 258 LNILQLSSNGISGPIPTEI 276



 Score = 94.0 bits (232), Expect = 1e-17
 Identities = 51/142 (35%), Positives = 80/142 (56%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L  L  L LS N ++G IP  + ++ SLQ +   +N L   IPS+L    +L  L+LS N
Sbjct: 255 LSKLNILQLSSNGISGPIPTEIFNISSLQEIDFSNNSLTGEIPSNLSHCRELRVLSLSFN 314

Query: 188 MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
             TG IP  I +L  +  + LS+N+L+G +P  IG    L  L +  N + GP+P  + N
Sbjct: 315 QFTGGIPQAIGSLSNLEGLYLSYNKLTGGIPREIGNLSNLNILQLGSNGISGPIPAEIFN 374

Query: 368 LINLEYLDLSSNRFSGAIPKSL 433
           + +L+ +D S+N  SG++P  +
Sbjct: 375 ISSLQIIDFSNNSLSGSLPMDI 396



 Score = 89.4 bits (220), Expect = 3e-16
 Identities = 52/143 (36%), Positives = 83/143 (58%), Gaps = 1/143 (0%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCL-GDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSS 184
           + +L  +  S+N L+GS+P  +   L +LQ LYL  N L+  +P++L    +L+ L+L+ 
Sbjct: 375 ISSLQIIDFSNNSLSGSLPMDICKHLPNLQGLYLLQNHLSGQLPTTLSLCGELLYLSLAV 434

Query: 185 NMITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLG 364
           N   GSIP EI NL ++ +I L  N L G +P++ G    L YL +  N L G +P+ + 
Sbjct: 435 NKFRGSIPREIGNLSKLEDISLRSNSLVGSIPTSFGNLMALKYLDLGMNFLTGTVPEAIF 494

Query: 365 NLINLEYLDLSSNRFSGAIPKSL 433
           N+  L+ L L  N  SG++P S+
Sbjct: 495 NISELQILVLVQNHLSGSLPPSI 517



 Score = 88.6 bits (218), Expect = 5e-16
 Identities = 54/145 (37%), Positives = 77/145 (53%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L  L  L L +N L G IP        L+ L L  NQ    IP ++ SL +L  L L+ N
Sbjct: 183 LVELQRLSLRNNSLTGEIPSNFSHCRELRGLSLSFNQFTGGIPQAIGSLCNLEELYLAFN 242

Query: 188 MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
            +TG IP EI NL ++  + LS N +SG +P+ I     L  +  S+N L G +P  L +
Sbjct: 243 KLTGGIPREIGNLSKLNILQLSSNGISGPIPTEIFNISSLQEIDFSNNSLTGEIPSNLSH 302

Query: 368 LINLEYLDLSSNRFSGAIPKSLEAL 442
              L  L LS N+F+G IP+++ +L
Sbjct: 303 CRELRVLSLSFNQFTGGIPQAIGSL 327



 Score = 83.6 bits (205), Expect = 2e-14
 Identities = 53/151 (35%), Positives = 85/151 (56%), Gaps = 9/151 (5%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGD-LESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSS 184
           +  L  L L  N L+GS+PP +G  L  L+ LY+ SN+ +  IP S+ +++ LI L +  
Sbjct: 496 ISELQILVLVQNHLSGSLPPSIGTWLPDLEGLYIGSNKFSGTIPMSISNMSKLIQLQVWD 555

Query: 185 NMITGSIPMEIQNLKQITEIGLSWNQLSG-YLPSTIG------GAQMLIYLSVSHNELQG 343
           N  TG++P ++ NL ++  + L+ NQL+  +L S +G        + L +L +  N  +G
Sbjct: 556 NSFTGNVPKDLGNLTKLEVLNLAANQLTNEHLASGVGFLTSLTNCKFLRHLWIDDNPFKG 615

Query: 344 PLPQTLGNL-INLEYLDLSSNRFSGAIPKSL 433
            LP +LGNL I LE    S+ +F G IP  +
Sbjct: 616 TLPNSLGNLPIALESFTASACQFRGTIPTGI 646



 Score = 83.2 bits (204), Expect = 2e-14
 Identities = 51/137 (37%), Positives = 76/137 (55%), Gaps = 1/137 (0%)
 Frame = +2

Query: 26  LYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSI 205
           L L+ NK  GSIP  +G+L  L+ + L SN L   IP+S  +L  L  L+L  N +TG++
Sbjct: 430 LSLAVNKFRGSIPREIGNLSKLEDISLRSNSLVGSIPTSFGNLMALKYLDLGMNFLTGTV 489

Query: 206 PMEIQNLKQITEIGLSWNQLSGYLPSTIG-GAQMLIYLSVSHNELQGPLPQTLGNLINLE 382
           P  I N+ ++  + L  N LSG LP +IG     L  L +  N+  G +P ++ N+  L 
Sbjct: 490 PEAIFNISELQILVLVQNHLSGSLPPSIGTWLPDLEGLYIGSNKFSGTIPMSISNMSKLI 549

Query: 383 YLDLSSNRFSGAIPKSL 433
            L +  N F+G +PK L
Sbjct: 550 QLQVWDNSFTGNVPKDL 566



 Score = 82.4 bits (202), Expect = 3e-14
 Identities = 51/143 (35%), Positives = 82/143 (57%), Gaps = 1/143 (0%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L  L  L LS+N  + S+P  +G  + LQ+L L +N+L   IP ++ +L+ L  L L +N
Sbjct: 14  LSFLVSLDLSNNYFHDSLPKDIGKCKELQQLNLFNNKLVGGIPEAICNLSKLEELYLGNN 73

Query: 188 MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
            + G IP ++ +L+ +  +    N L+G +P+TI     L+ +S+S+N L G LP+ +  
Sbjct: 74  ELIGEIPKKMNHLQNLKVLSFPMNNLTGSIPATIFNISSLLNISLSNNNLSGSLPKDMCY 133

Query: 368 L-INLEYLDLSSNRFSGAIPKSL 433
               L+ L+LSSN  SG IP  L
Sbjct: 134 ANPKLKELNLSSNHLSGKIPTGL 156



 Score = 82.4 bits (202), Expect = 3e-14
 Identities = 49/146 (33%), Positives = 84/146 (57%), Gaps = 1/146 (0%)
 Frame = +2

Query: 11   KNLGDLYLSDNKLNGSIPPCLGDLE-SLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
            K L  L++ DN   G++P  LG+L  +L+     + Q    IP+ + +LT+LI L+L +N
Sbjct: 601  KFLRHLWIDDNPFKGTLPNSLGNLPIALESFTASACQFRGTIPTGIGNLTNLIELDLGAN 660

Query: 188  MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
             +T SIP  +  L+++  + ++ N++ G +P+ +   + L YL +  N+L G +P   G+
Sbjct: 661  DLTRSIPTTLGRLQKLQRLHIAGNRIRGSIPNDLCHLKNLGYLHLXSNKLSGSIPSCFGD 720

Query: 368  LINLEYLDLSSNRFSGAIPKSLEALR 445
            L  L+ L L SN  +  IP SL +LR
Sbjct: 721  LPALQELFLDSNVLAFNIPTSLWSLR 746



 Score = 82.0 bits (201), Expect = 5e-14
 Identities = 46/129 (35%), Positives = 71/129 (55%)
 Frame = +2

Query: 47  LNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSIPMEIQNL 226
           L G+I P +G+L  L  L L +N  +  +P  +    +L  LNL +N + G IP  I NL
Sbjct: 3   LEGTIAPQVGNLSFLVSLDLSNNYFHDSLPKDIGKCKELQQLNLFNNKLVGGIPEAICNL 62

Query: 227 KQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNLINLEYLDLSSNR 406
            ++ E+ L  N+L G +P  +   Q L  LS   N L G +P T+ N+ +L  + LS+N 
Sbjct: 63  SKLEELYLGNNELIGEIPKKMNHLQNLKVLSFPMNNLTGSIPATIFNISSLLNISLSNNN 122

Query: 407 FSGAIPKSL 433
            SG++PK +
Sbjct: 123 LSGSLPKDM 131


>ref|XP_003633000.1| PREDICTED: LRR receptor-like serine/threonine-protein kinase
            GSO2-like [Vitis vinifera]
          Length = 1229

 Score =  173 bits (438), Expect = 2e-41
 Identities = 88/147 (59%), Positives = 110/147 (74%)
 Frame = +2

Query: 2    CQLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLS 181
            C LKNLG L+LS NKL+GSIP C GDL +LQ L+LDSN L   IP+SLWSL DL+ LNLS
Sbjct: 683  CHLKNLGYLHLSSNKLSGSIPSCFGDLPALQELFLDSNVLAFNIPTSLWSLRDLLVLNLS 742

Query: 182  SNMITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTL 361
            SN +TG++P E+ N+K IT + LS N +SG++P  +G  Q L  LS+S N+LQGP+P   
Sbjct: 743  SNFLTGNLPPEVGNMKSITTLDLSKNLVSGHIPRKMGEQQNLAKLSLSQNKLQGPIPIEF 802

Query: 362  GNLINLEYLDLSSNRFSGAIPKSLEAL 442
            G+L++LE LDLS N  SG IPKSLEAL
Sbjct: 803  GDLVSLESLDLSQNNLSGTIPKSLEAL 829



 Score =  105 bits (263), Expect = 3e-21
 Identities = 57/147 (38%), Positives = 93/147 (63%), Gaps = 1/147 (0%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L NL +LYLS NKL G IP  +G+L +L  L L SN ++  IP+ +++++ L  +  + N
Sbjct: 315 LSNLEELYLSHNKLTGGIPREIGNLSNLNILQLSSNGISGPIPAEIFNVSSLQVIAFTDN 374

Query: 188 MITGSIPMEI-QNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLG 364
            ++GS+P +I ++L  +  + LS N LSG LP+T+     L++LS+S N+ +G +P+ +G
Sbjct: 375 SLSGSLPKDICKHLPNLQGLSLSQNHLSGQLPTTLSLCGELLFLSLSFNKFRGSIPKEIG 434

Query: 365 NLINLEYLDLSSNRFSGAIPKSLEALR 445
           NL  LE + L +N   G+IP S   L+
Sbjct: 435 NLSKLEKIYLGTNSLIGSIPTSFGNLK 461



 Score =  104 bits (259), Expect = 9e-21
 Identities = 55/134 (41%), Positives = 80/134 (59%)
 Frame = +2

Query: 32  LSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSIPM 211
           L+ N   GSIP  +G+L  LQRL L +N     IP  L++++ L  LNL+ N + G IP 
Sbjct: 227 LAYNDFTGSIPSGIGNLVELQRLSLQNNSFTGEIPQLLFNISSLRFLNLAVNNLEGEIPS 286

Query: 212 EIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNLINLEYLD 391
            + + +++  + LS+NQ +G +P  IG    L  L +SHN+L G +P+ +GNL NL  L 
Sbjct: 287 NLSHCRELRVLSLSFNQFTGGIPQAIGSLSNLEELYLSHNKLTGGIPREIGNLSNLNILQ 346

Query: 392 LSSNRFSGAIPKSL 433
           LSSN  SG IP  +
Sbjct: 347 LSSNGISGPIPAEI 360



 Score = 98.2 bits (243), Expect = 6e-19
 Identities = 56/137 (40%), Positives = 82/137 (59%), Gaps = 1/137 (0%)
 Frame = +2

Query: 26  LYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSI 205
           L LS NK  GSIP  +G+L  L+++YL +N L   IP+S  +L  L  LNL  N +TG++
Sbjct: 418 LSLSFNKFRGSIPKEIGNLSKLEKIYLGTNSLIGSIPTSFGNLKALKFLNLGINNLTGTV 477

Query: 206 PMEIQNLKQITEIGLSWNQLSGYLPSTIG-GAQMLIYLSVSHNELQGPLPQTLGNLINLE 382
           P  I N+ ++  + +  N LSG LPS+IG     L  L ++ NE  G +P ++ N+  L 
Sbjct: 478 PEAIFNISKLQSLAMVKNHLSGSLPSSIGTWLSDLEGLFIAGNEFSGIIPMSISNMSKLT 537

Query: 383 YLDLSSNRFSGAIPKSL 433
            L LS+N F+G +PK L
Sbjct: 538 VLGLSANSFTGNVPKDL 554



 Score = 96.7 bits (239), Expect = 2e-18
 Identities = 54/139 (38%), Positives = 78/139 (56%)
 Frame = +2

Query: 17  LGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMIT 196
           L  L LS N L+G IP  LG    LQ + L  N     IPS + +L +L  L+L +N  T
Sbjct: 198 LKKLNLSSNHLSGKIPTGLGQCIQLQVISLAYNDFTGSIPSGIGNLVELQRLSLQNNSFT 257

Query: 197 GSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNLIN 376
           G IP  + N+  +  + L+ N L G +PS +   + L  LS+S N+  G +PQ +G+L N
Sbjct: 258 GEIPQLLFNISSLRFLNLAVNNLEGEIPSNLSHCRELRVLSLSFNQFTGGIPQAIGSLSN 317

Query: 377 LEYLDLSSNRFSGAIPKSL 433
           LE L LS N+ +G IP+ +
Sbjct: 318 LEELYLSHNKLTGGIPREI 336



 Score = 93.6 bits (231), Expect = 2e-17
 Identities = 58/142 (40%), Positives = 79/142 (55%), Gaps = 1/142 (0%)
 Frame = +2

Query: 11  KNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNM 190
           K L  L L +NKL G IP  + +L  L+ LYL +NQL   IP  +  L +L  L+   N 
Sbjct: 99  KELQQLNLFNNKLVGGIPEAICNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNN 158

Query: 191 ITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQ-MLIYLSVSHNELQGPLPQTLGN 367
           +TGSIP  I N+  +  I LS N LSG LP  +  A   L  L++S N L G +P  LG 
Sbjct: 159 LTGSIPATIFNISSLLNISLSNNNLSGSLPMDMCYANPKLKKLNLSSNHLSGKIPTGLGQ 218

Query: 368 LINLEYLDLSSNRFSGAIPKSL 433
            I L+ + L+ N F+G+IP  +
Sbjct: 219 CIQLQVISLAYNDFTGSIPSGI 240



 Score = 92.0 bits (227), Expect = 4e-17
 Identities = 55/173 (31%), Positives = 87/173 (50%), Gaps = 25/173 (14%)
 Frame = +2

Query: 2   CQLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIG---- 169
           C L  L +LYL +N+L G IP  +  L++L+ L    N L   IP+++++++ L+     
Sbjct: 120 CNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNNLTGSIPATIFNISSLLNISLS 179

Query: 170 ---------------------LNLSSNMITGSIPMEIQNLKQITEIGLSWNQLSGYLPST 286
                                LNLSSN ++G IP  +    Q+  I L++N  +G +PS 
Sbjct: 180 NNNLSGSLPMDMCYANPKLKKLNLSSNHLSGKIPTGLGQCIQLQVISLAYNDFTGSIPSG 239

Query: 287 IGGAQMLIYLSVSHNELQGPLPQTLGNLINLEYLDLSSNRFSGAIPKSLEALR 445
           IG    L  LS+ +N   G +PQ L N+ +L +L+L+ N   G IP +L   R
Sbjct: 240 IGNLVELQRLSLQNNSFTGEIPQLLFNISSLRFLNLAVNNLEGEIPSNLSHCR 292



 Score = 91.3 bits (225), Expect = 8e-17
 Identities = 53/142 (37%), Positives = 78/142 (54%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L  L  L L +N   G IP  L ++ SL+ L L  N L   IPS+L    +L  L+LS N
Sbjct: 243 LVELQRLSLQNNSFTGEIPQLLFNISSLRFLNLAVNNLEGEIPSNLSHCRELRVLSLSFN 302

Query: 188 MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
             TG IP  I +L  + E+ LS N+L+G +P  IG    L  L +S N + GP+P  + N
Sbjct: 303 QFTGGIPQAIGSLSNLEELYLSHNKLTGGIPREIGNLSNLNILQLSSNGISGPIPAEIFN 362

Query: 368 LINLEYLDLSSNRFSGAIPKSL 433
           + +L+ +  + N  SG++PK +
Sbjct: 363 VSSLQVIAFTDNSLSGSLPKDI 384



 Score = 87.8 bits (216), Expect = 8e-16
 Identities = 52/146 (35%), Positives = 87/146 (59%), Gaps = 1/146 (0%)
 Frame = +2

Query: 11   KNLGDLYLSDNKLNGSIPPCLGDLE-SLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
            K L +L++ +N   G++P  LG+L  +L+     + Q    IP+ + +LT+LI L+L +N
Sbjct: 589  KFLKNLWIGNNPFKGTLPNSLGNLPIALESFIASACQFRGTIPTRIGNLTNLIWLDLGAN 648

Query: 188  MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
             +TGSIP  +  LK++ ++ +  N+L G +P+ +   + L YL +S N+L G +P   G+
Sbjct: 649  DLTGSIPTTLGRLKKLQKLHIVGNRLRGSIPNDLCHLKNLGYLHLSSNKLSGSIPSCFGD 708

Query: 368  LINLEYLDLSSNRFSGAIPKSLEALR 445
            L  L+ L L SN  +  IP SL +LR
Sbjct: 709  LPALQELFLDSNVLAFNIPTSLWSLR 734



 Score = 84.7 bits (208), Expect = 7e-15
 Identities = 46/136 (33%), Positives = 76/136 (55%), Gaps = 1/136 (0%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L  L  +YL  N L GSIP   G+L++L+ L L  N L   +P ++++++ L  L +  N
Sbjct: 436 LSKLEKIYLGTNSLIGSIPTSFGNLKALKFLNLGINNLTGTVPEAIFNISKLQSLAMVKN 495

Query: 188 MITGSIPMEIQN-LKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLG 364
            ++GS+P  I   L  +  + ++ N+ SG +P +I     L  L +S N   G +P+ LG
Sbjct: 496 HLSGSLPSSIGTWLSDLEGLFIAGNEFSGIIPMSISNMSKLTVLGLSANSFTGNVPKDLG 555

Query: 365 NLINLEYLDLSSNRFS 412
           NL  L+ LDL+ N+ +
Sbjct: 556 NLTKLKVLDLAGNQLT 571



 Score = 83.2 bits (204), Expect = 2e-14
 Identities = 48/134 (35%), Positives = 73/134 (54%)
 Frame = +2

Query: 32  LSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSIPM 211
           LS+  L G+I P +G+L  L  L L +N  +  +P  +    +L  LNL +N + G IP 
Sbjct: 58  LSNMGLEGTIAPQVGNLSFLVSLDLSNNHFHGSLPKDIGKCKELQQLNLFNNKLVGGIPE 117

Query: 212 EIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNLINLEYLD 391
            I NL ++ E+ L  NQL G +P  +   Q L  LS   N L G +P T+ N+ +L  + 
Sbjct: 118 AICNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNNLTGSIPATIFNISSLLNIS 177

Query: 392 LSSNRFSGAIPKSL 433
           LS+N  SG++P  +
Sbjct: 178 LSNNNLSGSLPMDM 191



 Score = 64.3 bits (155), Expect = 1e-08
 Identities = 35/116 (30%), Positives = 64/116 (55%)
 Frame = +2

Query: 86  SLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSIPMEIQNLKQITEIGLSWNQL 265
           S+  + L +  L   I   + +L+ L+ L+LS+N   GS+P +I   K++ ++ L  N+L
Sbjct: 52  SVSAINLSNMGLEGTIAPQVGNLSFLVSLDLSNNHFHGSLPKDIGKCKELQQLNLFNNKL 111

Query: 266 SGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNLINLEYLDLSSNRFSGAIPKSL 433
            G +P  I     L  L + +N+L G +P+ + +L NL+ L    N  +G+IP ++
Sbjct: 112 VGGIPEAICNLSKLEELYLGNNQLIGEIPKKMNHLQNLKVLSFPMNNLTGSIPATI 167


>ref|XP_004150225.1| PREDICTED: probable LRR receptor-like serine/threonine-protein
           kinase At3g47570-like [Cucumis sativus]
          Length = 1092

 Score =  171 bits (434), Expect = 4e-41
 Identities = 84/147 (57%), Positives = 114/147 (77%)
 Frame = +2

Query: 2   CQLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLS 181
           CQL+NL +LYL++NKL+G+IP C  +L +L+ L L SN LNS +PSSLWSL+ ++ LNLS
Sbjct: 539 CQLENLDELYLANNKLSGAIPECFDNLSALRTLSLGSNNLNSTMPSSLWSLSYILHLNLS 598

Query: 182 SNMITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTL 361
           SN + GS+P+EI NL+ + +I +S NQLSG +PS+IGG   L+ LS+ HNEL+G +P + 
Sbjct: 599 SNSLRGSLPVEIGNLEVVLDIDVSKNQLSGEIPSSIGGLINLVNLSLLHNELEGSIPDSF 658

Query: 362 GNLINLEYLDLSSNRFSGAIPKSLEAL 442
           GNL+NLE LDLSSN  +G IP+SLE L
Sbjct: 659 GNLVNLEILDLSSNNLTGVIPRSLEKL 685



 Score =  103 bits (257), Expect = 1e-20
 Identities = 58/142 (40%), Positives = 84/142 (59%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L  L  + L +N  +G IP  +G L  ++ LYL  NQ + +IP+SL++LT LI LNL  N
Sbjct: 99  LPRLKMMSLGNNNFSGEIPTWIGRLPRMEELYLYGNQFSGLIPTSLFNLTSLIMLNLQEN 158

Query: 188 MITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGN 367
            ++GSIP EI NL  + ++ L+ NQL+  +P+ IG  Q L  L +  N   GP+P  + N
Sbjct: 159 QLSGSIPREIGNLTLLQDLYLNSNQLT-EIPTEIGTLQSLRTLDIEFNLFSGPIPLFIFN 217

Query: 368 LINLEYLDLSSNRFSGAIPKSL 433
           L +L  L LS N F G +P  +
Sbjct: 218 LSSLVILGLSGNNFIGGLPDDI 239



 Score = 95.1 bits (235), Expect = 5e-18
 Identities = 57/143 (39%), Positives = 79/143 (55%), Gaps = 1/143 (0%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCL-GDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSS 184
           L +L  L LS N   G +P  +  DL SL  LYL  NQL+  +PS+LW   +L  + L+ 
Sbjct: 218 LSSLVILGLSGNNFIGGLPDDICEDLPSLGGLYLSYNQLSGQLPSTLWKCENLEDVALAY 277

Query: 185 NMITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLG 364
           N  TGSIP  + NL ++ +I L  N LSG +P  +G  Q L YL++  N   G +P T+ 
Sbjct: 278 NQFTGSIPRNVGNLTRVKQIFLGVNYLSGEIPYELGYLQNLEYLAMQENFFNGTIPPTIF 337

Query: 365 NLINLEYLDLSSNRFSGAIPKSL 433
           NL  L  + L  N+ SG +P  L
Sbjct: 338 NLSKLNTIALVKNQLSGTLPADL 360



 Score = 93.2 bits (230), Expect = 2e-17
 Identities = 54/139 (38%), Positives = 80/139 (57%), Gaps = 1/139 (0%)
 Frame = +2

Query: 11  KNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNM 190
           +NL D+ L+ N+  GSIP  +G+L  +++++L  N L+  IP  L  L +L  L +  N 
Sbjct: 268 ENLEDVALAYNQFTGSIPRNVGNLTRVKQIFLGVNYLSGEIPYELGYLQNLEYLAMQENF 327

Query: 191 ITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIG-GAQMLIYLSVSHNELQGPLPQTLGN 367
             G+IP  I NL ++  I L  NQLSG LP+ +G G   L+ L +  NEL G +P+++ N
Sbjct: 328 FNGTIPPTIFNLSKLNTIALVKNQLSGTLPADLGVGLPNLVQLMLGRNELTGTIPESITN 387

Query: 368 LINLEYLDLSSNRFSGAIP 424
              L   D+  N FSG IP
Sbjct: 388 SSMLTLFDVGDNSFSGLIP 406



 Score = 91.3 bits (225), Expect = 8e-17
 Identities = 60/171 (35%), Positives = 93/171 (54%), Gaps = 25/171 (14%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSN 187
           L +L  L L +N+L+GSIP  +G+L  LQ LYL+SNQL  I P+ + +L  L  L++  N
Sbjct: 147 LTSLIMLNLQENQLSGSIPREIGNLTLLQDLYLNSNQLTEI-PTEIGTLQSLRTLDIEFN 205

Query: 188 MITGSIPMEIQNLKQITEIGLS-------------------------WNQLSGYLPSTIG 292
           + +G IP+ I NL  +  +GLS                         +NQLSG LPST+ 
Sbjct: 206 LFSGPIPLFIFNLSSLVILGLSGNNFIGGLPDDICEDLPSLGGLYLSYNQLSGQLPSTLW 265

Query: 293 GAQMLIYLSVSHNELQGPLPQTLGNLINLEYLDLSSNRFSGAIPKSLEALR 445
             + L  +++++N+  G +P+ +GNL  ++ + L  N  SG IP  L  L+
Sbjct: 266 KCENLEDVALAYNQFTGSIPRNVGNLTRVKQIFLGVNYLSGEIPYELGYLQ 316



 Score = 82.8 bits (203), Expect = 3e-14
 Identities = 60/167 (35%), Positives = 85/167 (50%), Gaps = 24/167 (14%)
 Frame = +2

Query: 5   QLKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSS 184
           +L  + +LYL  N+ +G IP  L +L SL  L L  NQL+  IP  + +LT L  L L+S
Sbjct: 122 RLPRMEELYLYGNQFSGLIPTSLFNLTSLIMLNLQENQLSGSIPREIGNLTLLQDLYLNS 181

Query: 185 NMIT-----------------------GSIPMEIQNLKQITEIGLSWNQLSGYLPSTI-G 292
           N +T                       G IP+ I NL  +  +GLS N   G LP  I  
Sbjct: 182 NQLTEIPTEIGTLQSLRTLDIEFNLFSGPIPLFIFNLSSLVILGLSGNNFIGGLPDDICE 241

Query: 293 GAQMLIYLSVSHNELQGPLPQTLGNLINLEYLDLSSNRFSGAIPKSL 433
               L  L +S+N+L G LP TL    NLE + L+ N+F+G+IP+++
Sbjct: 242 DLPSLGGLYLSYNQLSGQLPSTLWKCENLEDVALAYNQFTGSIPRNV 288



 Score = 76.3 bits (186), Expect = 3e-12
 Identities = 47/145 (32%), Positives = 79/145 (54%)
 Frame = +2

Query: 11  KNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNM 190
           K +  L  S   L G+ PP +G L  L  + + +N  +  +P  L +L  L  ++L +N 
Sbjct: 52  KRVTSLNFSFMGLTGTFPPEVGTLSFLTYVTIKNNSFHDPLPIELTNLPRLKMMSLGNNN 111

Query: 191 ITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQTLGNL 370
            +G IP  I  L ++ E+ L  NQ SG +P+++     LI L++  N+L G +P+ +GNL
Sbjct: 112 FSGEIPTWIGRLPRMEELYLYGNQFSGLIPTSLFNLTSLIMLNLQENQLSGSIPREIGNL 171

Query: 371 INLEYLDLSSNRFSGAIPKSLEALR 445
             L+ L L+SN+ +  IP  +  L+
Sbjct: 172 TLLQDLYLNSNQLT-EIPTEIGTLQ 195



 Score = 75.5 bits (184), Expect = 4e-12
 Identities = 42/109 (38%), Positives = 64/109 (58%)
 Frame = +2

Query: 26  LYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGSI 205
           L LS N L GS+P  +G+LE +  + +  NQL+  IPSS+  L +L+ L+L  N + GSI
Sbjct: 595 LNLSSNSLRGSLPVEIGNLEVVLDIDVSKNQLSGEIPSSIGGLINLVNLSLLHNELEGSI 654

Query: 206 PMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLP 352
           P    NL  +  + LS N L+G +P ++     L   +VS N+L+G +P
Sbjct: 655 PDSFGNLVNLEILDLSSNNLTGVIPRSLEKLSHLEQFNVSFNQLEGEIP 703



 Score = 72.8 bits (177), Expect = 3e-11
 Identities = 48/149 (32%), Positives = 76/149 (51%), Gaps = 8/149 (5%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLW-SLTDLIGLNLSS 184
           L+NL  L + +N  NG+IPP + +L  L  + L  NQL+  +P+ L   L +L+ L L  
Sbjct: 315 LQNLEYLAMQENFFNGTIPPTIFNLSKLNTIALVKNQLSGTLPADLGVGLPNLVQLMLGR 374

Query: 185 NMITGSIPMEIQNLKQITEIGLSWNQLSGYLPSTIGGAQMLIYLSVSHNELQGPLPQT-- 358
           N +TG+IP  I N   +T   +  N  SG +P+  G  + L ++++  N      P +  
Sbjct: 375 NELTGTIPESITNSSMLTLFDVGDNSFSGLIPNVFGRFENLRWINLELNNFTTESPPSER 434

Query: 359 -----LGNLINLEYLDLSSNRFSGAIPKS 430
                L NL +L  L+LS N  +  +P S
Sbjct: 435 GIFSFLTNLTSLVRLELSHNPLNIFLPSS 463



 Score = 67.0 bits (162), Expect = 2e-09
 Identities = 35/87 (40%), Positives = 54/87 (62%)
 Frame = +2

Query: 23  DLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLSSNMITGS 202
           D+ +S N+L+G IP  +G L +L  L L  N+L   IP S  +L +L  L+LSSN +TG 
Sbjct: 618 DIDVSKNQLSGEIPSSIGGLINLVNLSLLHNELEGSIPDSFGNLVNLEILDLSSNNLTGV 677

Query: 203 IPMEIQNLKQITEIGLSWNQLSGYLPS 283
           IP  ++ L  + +  +S+NQL G +P+
Sbjct: 678 IPRSLEKLSHLEQFNVSFNQLEGEIPN 704



 Score = 64.3 bits (155), Expect = 1e-08
 Identities = 46/155 (29%), Positives = 79/155 (50%), Gaps = 9/155 (5%)
 Frame = +2

Query: 8   LKNLGDLYLSDNKLNGSIPPCLGDLESLQRLYLDSNQLNSIIPSSLWSLTDLIGLNLS-S 184
           L NL  L L  N+L G+IP  + +   L    +  N  + +IP+      +L  +NL  +
Sbjct: 364 LPNLVQLMLGRNELTGTIPESITNSSMLTLFDVGDNSFSGLIPNVFGRFENLRWINLELN 423

Query: 185 NMITGSIPME------IQNLKQITEIGLSWNQLSGYLPST-IGGAQMLIYLSVSHNELQG 343
           N  T S P E      + NL  +  + LS N L+ +LPS+ +  +    YLS+ +  ++G
Sbjct: 424 NFTTESPPSERGIFSFLTNLTSLVRLELSHNPLNIFLPSSFVNFSSSFQYLSMVNTGIKG 483

Query: 344 PLPQTLGNLI-NLEYLDLSSNRFSGAIPKSLEALR 445
            +P+ +GN + +L  L +  N+ +G IP S+  L+
Sbjct: 484 MIPKDIGNFLRSLIVLVMDDNQITGTIPTSIGKLK 518