BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Angelica22_contig00022792
         (1799 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudi...   628   e-177
gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]      626   e-177
gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P...   608   e-171
ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P...   608   e-171
ref|XP_003524231.1| PREDICTED: cytochrome P450 716B2-like [Glyci...   569   e-159

>gb|ABD60225.1| ent-kaurenoic acid 13-hydroxylase [Stevia rebaudiana]
            gi|189418962|gb|ACD93722.1| ent-kaurenoic acid
            13-hydroxylase [Stevia rebaudiana]
          Length = 476

 Score =  628 bits (1619), Expect = e-177
 Identities = 301/467 (64%), Positives = 364/467 (77%), Gaps = 4/467 (0%)
 Frame = +1

Query: 64   LVTILFVTTKIAK--KGNKNLPPGKYGWPIMGETLSLLQAGWNGTPEKFIKERVEKHGD- 234
            L  I FV  K+ K  K   NLPPG +GWP +GETL+LL+AGW+  PE+F++ER++KHG  
Sbjct: 10   LFLIFFVFWKVYKHQKTKINLPPGSFGWPFLGETLALLRAGWDSEPERFVRERIKKHGSP 69

Query: 235  -VFKTSLLGDSIVVFSGVAGNKFLFGNEGKTVALWWPKSVNKLFGSCLITSSGEEAKWMR 411
             VFKTSL GD   V  G AGNKFLF NE K VA WWP  V KLFG  L+T  G+EAKWMR
Sbjct: 70   LVFKTSLFGDRFAVLCGPAGNKFLFCNENKLVASWWPVPVRKLFGKSLLTIRGDEAKWMR 129

Query: 412  KMLHSFLSPDAFSRLYISIMDLVTQKHIETHWQGKQEVKVYNTIKLYTFELACLLFMSLE 591
            KML S+L PDAF+  Y   MD+VT++HI+ HW+GK+EV V+ T+KLY FELAC LFM+L+
Sbjct: 130  KMLLSYLGPDAFATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFELACRLFMNLD 189

Query: 592  DPNHIKKLASHFNIFLKGIIQIPLNVPGTRFYNAMRSANAIRKDLHAITRQRRLDLELKT 771
            DPNHI KL S FNIFLKGII++P++VPGTRFY++ ++A AIR +L  + + R+L+L+   
Sbjct: 190  DPNHIAKLGSLFNIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKARKLELKEGK 249

Query: 772  ASPSQDLLSHLLVSSDEHGRFLTEAEIVNNILTLLFAGHDTSSVSITLVMKTLAEKPQVY 951
            AS SQDLLSHLL S DE+G FLTE EIV+NIL LLFAGHDTS++SITL+MKTL E   VY
Sbjct: 250  ASSSQDLLSHLLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKTLGEHSDVY 309

Query: 952  QKVYDEHMDIAAAKGHEELLQWDDIQKMKYTWNVVSEVMRLTPPVTGAFREALVDFTYAG 1131
             KV  E ++I+  K   E L+W+DIQKMKY+W+V+ EVMRL PPV G +REALVD  YAG
Sbjct: 310  DKVLKEQLEISKTKEAWESLKWEDIQKMKYSWSVICEVMRLNPPVIGTYREALVDIDYAG 369

Query: 1132 YTIPRGWKLYWSTVSTNKDEKYFPDPTEFDASRFEGAGPTPYSYVPFGGGPRMCIGKEFA 1311
            YTIP+GWKL+WS VST +DE  F D T FD SRFEGAGPTP+++VPFGGGPRMC+GKEFA
Sbjct: 370  YTIPKGWKLHWSAVSTQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGPRMCLGKEFA 429

Query: 1312 RMEILVFLHNLVKRFKWDLLIADEKIEYDPMPTPVKGLPISLHPHNV 1452
            R+E+L FLHN+V  FKWDLLI DEKIEYDPM TP KGLPI LHPH V
Sbjct: 430  RLEVLAFLHNIVTNFKWDLLIPDEKIEYDPMATPAKGLPIRLHPHQV 476


>gb|ACL10147.1| cytochrome P450 monooxygenase [Stevia rebaudiana]
          Length = 476

 Score =  626 bits (1615), Expect = e-177
 Identities = 301/467 (64%), Positives = 364/467 (77%), Gaps = 4/467 (0%)
 Frame = +1

Query: 64   LVTILFVTTKIAK--KGNKNLPPGKYGWPIMGETLSLLQAGWNGTPEKFIKERVEKHGD- 234
            L  I FV  K+ K  K   NLPPG +GWP +GETL+LL+AGW+  PE+F++ER++KHG  
Sbjct: 10   LFLIFFVFWKVYKHQKTKINLPPGSFGWPFLGETLALLRAGWDSEPERFVRERIKKHGSP 69

Query: 235  -VFKTSLLGDSIVVFSGVAGNKFLFGNEGKTVALWWPKSVNKLFGSCLITSSGEEAKWMR 411
             VFKTSL GD   V  G AGNKFLF NE K VA WWP  V KLFG  L+T  G+EAKWMR
Sbjct: 70   LVFKTSLFGDRFAVLCGPAGNKFLFCNENKLVASWWPVPVRKLFGKSLLTIRGDEAKWMR 129

Query: 412  KMLHSFLSPDAFSRLYISIMDLVTQKHIETHWQGKQEVKVYNTIKLYTFELACLLFMSLE 591
            KML S+L PDAF+  Y   MD+VT++HI+ HW+GK+EV V+ T+KLY FELAC LFM+L+
Sbjct: 130  KMLLSYLGPDAFATHYAVTMDVVTRRHIDVHWRGKEEVNVFQTVKLYAFELACRLFMNLD 189

Query: 592  DPNHIKKLASHFNIFLKGIIQIPLNVPGTRFYNAMRSANAIRKDLHAITRQRRLDLELKT 771
            DPNHI KL S FNIFLKGII++P++VPGTRFY++ ++A AIR +L  + + R+L+L+   
Sbjct: 190  DPNHIAKLGSLFNIFLKGIIELPIDVPGTRFYSSKKAAAAIRIELKKLIKARKLELKEGK 249

Query: 772  ASPSQDLLSHLLVSSDEHGRFLTEAEIVNNILTLLFAGHDTSSVSITLVMKTLAEKPQVY 951
             S SQDLLSHLL S DE+G FLTE EIV+NIL LLFAGHDTS++SITL+MK L E   VY
Sbjct: 250  PSSSQDLLSHLLTSPDENGMFLTEEEIVDNILLLLFAGHDTSALSITLLMKALGEHSDVY 309

Query: 952  QKVYDEHMDIAAAKGHEELLQWDDIQKMKYTWNVVSEVMRLTPPVTGAFREALVDFTYAG 1131
             KV  E ++I+  K   ELL+W+DIQKMKY+W+VV EVMRL PPV GA+REALVD  YAG
Sbjct: 310  DKVLKEQLEISKGKEAGELLKWEDIQKMKYSWSVVCEVMRLNPPVIGAYREALVDIDYAG 369

Query: 1132 YTIPRGWKLYWSTVSTNKDEKYFPDPTEFDASRFEGAGPTPYSYVPFGGGPRMCIGKEFA 1311
            YTIP+GWKL+WS VST +DE  F D T FD SRFEGAGPTP+++VPFGGGPRMC+GKEFA
Sbjct: 370  YTIPKGWKLHWSAVSTQRDEANFEDVTRFDPSRFEGAGPTPFTFVPFGGGPRMCLGKEFA 429

Query: 1312 RMEILVFLHNLVKRFKWDLLIADEKIEYDPMPTPVKGLPISLHPHNV 1452
            R+E+L FLHN+V  FKWDLLI DEKIEYDP+ TP KGLPI LHPH V
Sbjct: 430  RLEVLAFLHNIVINFKWDLLIPDEKIEYDPIATPAKGLPIRLHPHQV 476


>gb|ABC94482.1| putative taxane 13-alpha-hydroxylase cytochrome P450 [Artemisia
            annua]
          Length = 480

 Score =  608 bits (1569), Expect = e-171
 Identities = 286/450 (63%), Positives = 354/450 (78%), Gaps = 3/450 (0%)
 Frame = +1

Query: 112  KNLPPGKYGWPIMGETLSLLQAGWNGTPEKFIKERVEKHGD--VFKTSLLGDSIVVFSGV 285
            KNLPPG +GWP +GETL+ +++   G PE+F KER+EK+G   VFKTS+ G+ + VF G 
Sbjct: 31   KNLPPGSFGWPFIGETLAYIRSKRGGDPERFTKERIEKYGSTLVFKTSVAGERMAVFCGP 90

Query: 286  AGNKFLFGNEGKTVALWWPKSVNKLFGSCLITSSGEEAKWMRKMLHSFLSPDAFSRLYIS 465
             GNKFLFGNE K VA WWP SV  LF  CLIT  G+EAKW+RKM+ ++L PDA S  Y  
Sbjct: 91   EGNKFLFGNENKLVASWWPNSVRILFEKCLITIRGDEAKWLRKMMFAYLGPDALSNRYTG 150

Query: 466  IMDLVTQKHIETHWQGKQEVKVYNTIKLYTFELACLLFMSLEDPNHIKKLASHFNIFLKG 645
             M++VT+ HI+ HWQGK E+KV+ T++ Y FELAC LF+SL+DP H+ +L + FN FLKG
Sbjct: 151  TMEVVTRLHIQNHWQGKSELKVFETVRPYLFELACRLFLSLDDPKHVAELGTLFNTFLKG 210

Query: 646  IIQIPLNVPGTRFYNAMRSANAIRKDLHAITRQRRLDLELKTASPS-QDLLSHLLVSSDE 822
            + ++P+N+PGTRFY A R+ANAI+K L  I +QRR  L+ +  S S +DLLSHLLVSSDE
Sbjct: 211  LTELPINIPGTRFYRAKRAANAIKKQLIVIIKQRRQALKQEDQSSSFEDLLSHLLVSSDE 270

Query: 823  HGRFLTEAEIVNNILTLLFAGHDTSSVSITLVMKTLAEKPQVYQKVYDEHMDIAAAKGHE 1002
            +GRFL+EAEI NN+L LLFAGHDTS+VSITL+MK+LAE PQVY  V  E + I  AK   
Sbjct: 271  NGRFLSEAEIANNVLLLLFAGHDTSAVSITLLMKSLAEHPQVYDNVLKEQLGILEAKAPG 330

Query: 1003 ELLQWDDIQKMKYTWNVVSEVMRLTPPVTGAFREALVDFTYAGYTIPRGWKLYWSTVSTN 1182
            E+L W+DIQKM+Y+W VV EVMRL PPV G+FREALVDF YAGYTIP+GWK+ WS V T+
Sbjct: 331  EMLNWEDIQKMRYSWYVVCEVMRLIPPVVGSFREALVDFEYAGYTIPKGWKIIWSAVMTH 390

Query: 1183 KDEKYFPDPTEFDASRFEGAGPTPYSYVPFGGGPRMCIGKEFARMEILVFLHNLVKRFKW 1362
            K+E  FP+ T+FD SRFEGAGPTP++YVPFGGGPRMC+GKE AR+ ILVFLHN++ +FKW
Sbjct: 391  KEENNFPNATKFDPSRFEGAGPTPFTYVPFGGGPRMCLGKELARVRILVFLHNIMTKFKW 450

Query: 1363 DLLIADEKIEYDPMPTPVKGLPISLHPHNV 1452
            DLLI DEKI YDP+ TPVKGLP+ LHPH V
Sbjct: 451  DLLIPDEKIGYDPLATPVKGLPVRLHPHQV 480


>ref|XP_003632173.1| PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 716B2-like [Vitis
            vinifera]
          Length = 473

 Score =  608 bits (1567), Expect = e-171
 Identities = 292/467 (62%), Positives = 370/467 (79%), Gaps = 2/467 (0%)
 Frame = +1

Query: 52   IISTLVTILFVTTKIAKKGN-KNLPPGKYGWPIMGETLSLLQAGWNGTPEKFIKERVEKH 228
            +I  L ++ F+  KI K G   NLPPG  GWP +GETL  L++G +G P++FI+ER++K+
Sbjct: 9    VILALFSLHFIA-KILKAGKFSNLPPGSLGWPRIGETLVFLRSGEDGEPQRFIRERMDKY 67

Query: 229  GD-VFKTSLLGDSIVVFSGVAGNKFLFGNEGKTVALWWPKSVNKLFGSCLITSSGEEAKW 405
               VFKTSLLG+ ++VF G AGNKFLFGNE K V++WWP SV KLF SCL+T++G+EAK 
Sbjct: 68   DSGVFKTSLLGEPMMVFCGPAGNKFLFGNENKLVSVWWPSSVRKLFRSCLVTATGDEAKR 127

Query: 406  MRKMLHSFLSPDAFSRLYISIMDLVTQKHIETHWQGKQEVKVYNTIKLYTFELACLLFMS 585
            MR+ML +FL+PDA  R Y+  MDLVTQ HI T W+G +E++VY  IKLYTFELAC LF S
Sbjct: 128  MRRMLLTFLNPDALKR-YVETMDLVTQHHIRTQWEGNEEMQVYPIIKLYTFELACRLFAS 186

Query: 586  LEDPNHIKKLASHFNIFLKGIIQIPLNVPGTRFYNAMRSANAIRKDLHAITRQRRLDLEL 765
            ++DP HI KLA+HFN+FLKGII  P+N PGT+F  A ++ANAIR++L  +  +RR+ LE 
Sbjct: 187  MDDPGHISKLAAHFNVFLKGIIDFPINFPGTKFXYATKAANAIREELRVMPMERRVALEE 246

Query: 766  KTASPSQDLLSHLLVSSDEHGRFLTEAEIVNNILTLLFAGHDTSSVSITLVMKTLAEKPQ 945
            KTAS +QDLLSHLLV++D  GRFLTE EI++NIL LLFAGHDT++ +ITL++K L E P+
Sbjct: 247  KTASATQDLLSHLLVTADASGRFLTEMEIIDNILLLLFAGHDTTASAITLLIKYLGELPE 306

Query: 946  VYQKVYDEHMDIAAAKGHEELLQWDDIQKMKYTWNVVSEVMRLTPPVTGAFREALVDFTY 1125
            VY KV  E +DI  +K   ELLQW D QKM+Y+W+V SEVMRL+PP +G+FREALVDF+Y
Sbjct: 307  VYAKVLREKIDITESKEPGELLQWKDXQKMRYSWHVASEVMRLSPPASGSFREALVDFSY 366

Query: 1126 AGYTIPRGWKLYWSTVSTNKDEKYFPDPTEFDASRFEGAGPTPYSYVPFGGGPRMCIGKE 1305
            AGY IP+GWKLYW T ST +D  +F +P  FDASRFEGAGP P+SYVPFGGGPRMC+G+E
Sbjct: 367  AGYNIPKGWKLYWGTGSTQRDPAFFRNPDNFDASRFEGAGPAPFSYVPFGGGPRMCLGQE 426

Query: 1306 FARMEILVFLHNLVKRFKWDLLIADEKIEYDPMPTPVKGLPISLHPH 1446
            FAR++ILVF+HN+VKRF WDLL  DEKIE+ PM  P++GLPI LHPH
Sbjct: 427  FARLQILVFMHNIVKRFTWDLLNPDEKIEHSPMLAPLEGLPIRLHPH 473


>ref|XP_003524231.1| PREDICTED: cytochrome P450 716B2-like [Glycine max]
          Length = 486

 Score =  569 bits (1466), Expect = e-159
 Identities = 269/452 (59%), Positives = 350/452 (77%), Gaps = 1/452 (0%)
 Frame = +1

Query: 91   KIAKKGNKNLPPGKYGWPIMGETLSLLQAGWNGTPEKFIKERVEKHGD-VFKTSLLGDSI 267
            ++ K  N NLPPG+ GWP++GETL  L+    G   +FI+ER EK+   VFKTS+ GD +
Sbjct: 33   RLGKHPNLNLPPGRLGWPVVGETLEFLRTMNEGNVLRFIQERKEKYDSRVFKTSMFGDPV 92

Query: 268  VVFSGVAGNKFLFGNEGKTVALWWPKSVNKLFGSCLITSSGEEAKWMRKMLHSFLSPDAF 447
            V+F G AGNKFLF NE K V +WWP SV +L    L+   G+EAK +R++L SFL+ +  
Sbjct: 93   VLFCGPAGNKFLFSNENKNVQVWWPSSVRRLLRLSLVNKVGDEAKMVRRLLMSFLNAETL 152

Query: 448  SRLYISIMDLVTQKHIETHWQGKQEVKVYNTIKLYTFELACLLFMSLEDPNHIKKLASHF 627
             R Y+  MD + Q+HI+T+W+GK++V VY  ++LYTFELAC LF+S+ED +HI KL+  F
Sbjct: 153  -RNYLPKMDSIAQRHIDTYWEGKEQVCVYPIVQLYTFELACCLFLSIEDSDHISKLSLKF 211

Query: 628  NIFLKGIIQIPLNVPGTRFYNAMRSANAIRKDLHAITRQRRLDLELKTASPSQDLLSHLL 807
            + FLKGII  PLNVPGTRFY AM++A+ IRK++  I ++R++DLE K  SP+QDLLSH+L
Sbjct: 212  DEFLKGIIGFPLNVPGTRFYRAMKAADVIRKEIKMILKKRKVDLEEKRVSPTQDLLSHML 271

Query: 808  VSSDEHGRFLTEAEIVNNILTLLFAGHDTSSVSITLVMKTLAEKPQVYQKVYDEHMDIAA 987
            V+SD  GRF+TE EI++NIL LLFAGHDTS   ++LVMK L + PQVY+ V +E ++I+ 
Sbjct: 272  VTSDPSGRFMTEMEILDNILLLLFAGHDTSRSVLSLVMKYLGQLPQVYEHVLEEQLEISQ 331

Query: 988  AKGHEELLQWDDIQKMKYTWNVVSEVMRLTPPVTGAFREALVDFTYAGYTIPRGWKLYWS 1167
             K   +LLQW+D+QKMKY+WNV SEVMRL+PPV+GA+REA+ DFTYA Y IP+GWKL+W+
Sbjct: 332  GKEAGQLLQWEDVQKMKYSWNVASEVMRLSPPVSGAYREAIKDFTYADYNIPKGWKLHWN 391

Query: 1168 TVSTNKDEKYFPDPTEFDASRFEGAGPTPYSYVPFGGGPRMCIGKEFARMEILVFLHNLV 1347
            T S++KD   F +P  FDASRFEGAGPTP+SYVPFGGGPRMC+G EFAR+EILVF+HN+V
Sbjct: 392  TGSSHKDPTLFSNPETFDASRFEGAGPTPFSYVPFGGGPRMCLGLEFARLEILVFMHNIV 451

Query: 1348 KRFKWDLLIADEKIEYDPMPTPVKGLPISLHP 1443
            KRFKWDL+I DE  +YDPM  P+KGL I LHP
Sbjct: 452  KRFKWDLVIPDEMFKYDPMLEPIKGLAIRLHP 483