BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Angelica22_contig00020189
         (1817 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277794.1| PREDICTED: EH domain-containing protein 1 [V...   909   0.0  
ref|NP_566657.1| EPS15 homology domain 1 protein [Arabidopsis th...   890   0.0  
ref|XP_002885363.1| calcium-binding EF hand family protein [Arab...   886   0.0  
ref|XP_003617631.1| EH-domain-containing protein [Medicago trunc...   880   0.0  
ref|XP_004149345.1| PREDICTED: EH domain-containing protein 1-li...   879   0.0  

>ref|XP_002277794.1| PREDICTED: EH domain-containing protein 1 [Vitis vinifera]
            gi|297737489|emb|CBI26690.3| unnamed protein product
            [Vitis vinifera]
          Length = 545

 Score =  909 bits (2349), Expect = 0.0
 Identities = 447/544 (82%), Positives = 487/544 (89%)
 Frame = +3

Query: 12   MEINSSSISRCSKEHHKIYQDWFNFADSDSDGRLTGADAIKFFSMSNLPRPDLKQVWAIA 191
            MEI+S  IS CSK++  IYQ+WFNFADSDSDGR+TG DAIKFF+MSNLPRPDLKQVWAIA
Sbjct: 1    MEIDSGPISSCSKQNQMIYQEWFNFADSDSDGRITGNDAIKFFAMSNLPRPDLKQVWAIA 60

Query: 192  DSKRQGFLGFNEFVTAMQLVSLAQSGQPITSDLLNSEVDFENLNLPTMDGLDSLLAKQKR 371
            D+KRQGFLG  EF+TAMQLVSLAQ+G  IT DLL+SEVD  NLN P M+GL +LLAK+KR
Sbjct: 61   DTKRQGFLGLKEFITAMQLVSLAQAGHAITQDLLHSEVDLGNLNPPQMEGLAALLAKKKR 120

Query: 372  ATKTSELELNSSPVQPXXXXXXXXXXXXXXXXXXXXXXXIIDGLKKLYIQKVKPLEVTYR 551
            A KTS+ ++N S                           IIDGLK+LYIQK+KPLEVTYR
Sbjct: 121  AHKTSDPDINGSSQPQPSPTASWFTSKSSKKVPLSSVTSIIDGLKRLYIQKLKPLEVTYR 180

Query: 552  FNDFVSPLLTSSDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPGAHIGPEPTTDRFVVVM 731
            FNDF SPLLT+SDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPGAHIGPEPTTDRFVVVM
Sbjct: 181  FNDFASPLLTNSDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPGAHIGPEPTTDRFVVVM 240

Query: 732  NGPDERSIPGNTVAVQADMPYSGLTTFGTAFLSKFECSQMPHPLLEHITFVDTPGVLSGE 911
            +GPDERSIPGNT+AVQADMPYSGLTTFGTAFLSKFECSQMPH LLEHITFVDTPGVLSGE
Sbjct: 241  SGPDERSIPGNTIAVQADMPYSGLTTFGTAFLSKFECSQMPHSLLEHITFVDTPGVLSGE 300

Query: 912  KQRTQRSYDFTGVTAWFAAKCDLILLLFDPHKLDISDEFKRVISSLRGHDDKIRVVLNKA 1091
            KQRTQR+YDFTGVT+WFAAKCDLILLLFDPHKLD+SDEFKRVISSL GHDDKIRVVLNKA
Sbjct: 301  KQRTQRAYDFTGVTSWFAAKCDLILLLFDPHKLDVSDEFKRVISSLHGHDDKIRVVLNKA 360

Query: 1092 DQVDTQQLMRVYGALMWSLGKVLNTPEVMRVYIGSFNDKPINIATAGPLGQELFEREQED 1271
            DQVDTQQLMRVYGALMWSLGKVLNTPEVMRVYIGSFNDKP+N A  GP+G+ELFE+EQE+
Sbjct: 361  DQVDTQQLMRVYGALMWSLGKVLNTPEVMRVYIGSFNDKPVNEAAVGPIGKELFEKEQEN 420

Query: 1272 LLTDLRDIPKKACDRRINEFVKRARAAKIHSYIISHLKKEMPAMMGKAKAQQRLIDNLED 1451
            LL+DL+DIPKKACDRRINEFVKRARAAKIH+YIISHLKKEMP MMGKAK QQRLIDNLED
Sbjct: 421  LLSDLKDIPKKACDRRINEFVKRARAAKIHAYIISHLKKEMPTMMGKAKTQQRLIDNLED 480

Query: 1452 EFKKVQRDYHLPAGDFPSVEHFREILNGYNIDKFEKLKPKMIQTVDDMLGYDIPNLLKSF 1631
            EF KVQR++HLPAGDFP+V+HFRE+L+GY+IDKFE+LKPKMIQTVDDMLGYDIP LLKSF
Sbjct: 481  EFAKVQREFHLPAGDFPNVDHFREVLSGYSIDKFERLKPKMIQTVDDMLGYDIPELLKSF 540

Query: 1632 SNPY 1643
             NPY
Sbjct: 541  RNPY 544


>ref|NP_566657.1| EPS15 homology domain 1 protein [Arabidopsis thaliana]
            gi|79313303|ref|NP_001030731.1| EPS15 homology domain 1
            protein [Arabidopsis thaliana] gi|14334440|gb|AAK59418.1|
            unknown protein [Arabidopsis thaliana]
            gi|28394001|gb|AAO42408.1| unknown protein [Arabidopsis
            thaliana] gi|222424046|dbj|BAH19984.1| AT3G20290
            [Arabidopsis thaliana] gi|332642838|gb|AEE76359.1| EPS15
            homology domain 1 protein [Arabidopsis thaliana]
            gi|332642839|gb|AEE76360.1| EPS15 homology domain 1
            protein [Arabidopsis thaliana]
          Length = 545

 Score =  890 bits (2300), Expect = 0.0
 Identities = 430/544 (79%), Positives = 485/544 (89%)
 Frame = +3

Query: 12   MEINSSSISRCSKEHHKIYQDWFNFADSDSDGRLTGADAIKFFSMSNLPRPDLKQVWAIA 191
            MEI S +   CSKE+  IY++WF F+DSD DGR+TG DAIKFF+MSNLPRP+LKQ+WAIA
Sbjct: 1    MEIESVAAGSCSKENQMIYKEWFEFSDSDGDGRITGNDAIKFFTMSNLPRPELKQIWAIA 60

Query: 192  DSKRQGFLGFNEFVTAMQLVSLAQSGQPITSDLLNSEVDFENLNLPTMDGLDSLLAKQKR 371
            DSKRQG+LGF EF+ AMQLVSLAQ+G  I+ ++L S+VDF+N+N PTM+GL  L+AK+K 
Sbjct: 61   DSKRQGYLGFKEFIVAMQLVSLAQTGHEISHEVLISDVDFKNINPPTMEGLGVLMAKKKH 120

Query: 372  ATKTSELELNSSPVQPXXXXXXXXXXXXXXXXXXXXXXXIIDGLKKLYIQKVKPLEVTYR 551
            ++K+S+  +N SP                          I+DGLK+LYIQK+KPLEV YR
Sbjct: 121  SSKSSDPNMNGSPAADTSLTAHWFSSKSSKKISLSSVTSIVDGLKRLYIQKLKPLEVAYR 180

Query: 552  FNDFVSPLLTSSDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPGAHIGPEPTTDRFVVVM 731
            FNDFVSPLLT+SDFDAKPMVMLLGQYSTGKTTFIKHLLKS+YPGAHIGPEPTTDRFVVVM
Sbjct: 181  FNDFVSPLLTNSDFDAKPMVMLLGQYSTGKTTFIKHLLKSTYPGAHIGPEPTTDRFVVVM 240

Query: 732  NGPDERSIPGNTVAVQADMPYSGLTTFGTAFLSKFECSQMPHPLLEHITFVDTPGVLSGE 911
            +GPDERSIPGNTVAVQADMP+SGLTTFGTAFLSKFECSQMPHPLLEH+TFVDTPGVLSGE
Sbjct: 241  SGPDERSIPGNTVAVQADMPFSGLTTFGTAFLSKFECSQMPHPLLEHVTFVDTPGVLSGE 300

Query: 912  KQRTQRSYDFTGVTAWFAAKCDLILLLFDPHKLDISDEFKRVISSLRGHDDKIRVVLNKA 1091
            KQRTQR+YDFTGVT+WFA+KCDLILLLFDPHKLD+SDEFKRVISSLRGHDDKIRVVLNKA
Sbjct: 301  KQRTQRAYDFTGVTSWFASKCDLILLLFDPHKLDVSDEFKRVISSLRGHDDKIRVVLNKA 360

Query: 1092 DQVDTQQLMRVYGALMWSLGKVLNTPEVMRVYIGSFNDKPINIATAGPLGQELFEREQED 1271
            DQVDTQQLMRVYGALMWSLGKVLNTPEV RVYIGSF+DKPIN A  GP+G+ELFE+EQ+D
Sbjct: 361  DQVDTQQLMRVYGALMWSLGKVLNTPEVSRVYIGSFSDKPINEAATGPIGRELFEKEQDD 420

Query: 1272 LLTDLRDIPKKACDRRINEFVKRARAAKIHSYIISHLKKEMPAMMGKAKAQQRLIDNLED 1451
            LL DL+DIPKKACDRRINEFVKRARAAKIH+YIISHLKKEMPA+MGKAKAQQ+LIDNLED
Sbjct: 421  LLADLKDIPKKACDRRINEFVKRARAAKIHAYIISHLKKEMPAIMGKAKAQQKLIDNLED 480

Query: 1452 EFKKVQRDYHLPAGDFPSVEHFREILNGYNIDKFEKLKPKMIQTVDDMLGYDIPNLLKSF 1631
            EF KVQR++HLP GDFP+V+HFRE+L+GYNIDKFEKLKPKM+QTVDDMLGYDIP LLK+F
Sbjct: 481  EFGKVQREHHLPKGDFPNVDHFREVLSGYNIDKFEKLKPKMLQTVDDMLGYDIPELLKNF 540

Query: 1632 SNPY 1643
             NPY
Sbjct: 541  KNPY 544


>ref|XP_002885363.1| calcium-binding EF hand family protein [Arabidopsis lyrata subsp.
            lyrata] gi|297331203|gb|EFH61622.1| calcium-binding EF
            hand family protein [Arabidopsis lyrata subsp. lyrata]
          Length = 550

 Score =  886 bits (2289), Expect = 0.0
 Identities = 431/549 (78%), Positives = 485/549 (88%), Gaps = 5/549 (0%)
 Frame = +3

Query: 12   MEINSSSISRCSKEHHKIYQDWFNFADSDSDGRLTGADAIKFFSMSNLPRPDLKQVWAIA 191
            MEI S +   CSKE+  IY++WF FADSD DGR+TG DAIKFF+MSNLPRP+LKQ+WAIA
Sbjct: 1    MEIESVAAGSCSKENQLIYKEWFEFADSDGDGRITGNDAIKFFTMSNLPRPELKQIWAIA 60

Query: 192  DSKRQGFLGFNEFVTAMQLVSLAQSGQPITSDLLNSEVDFENLNLPTMDGLDSLLAKQKR 371
            DSKRQG+L F +F+ AMQLVSLAQ+G  I+ ++LNS+VDF+N+N PTM+GL  L+AK+K 
Sbjct: 61   DSKRQGYLAFKDFIVAMQLVSLAQTGHEISHEILNSDVDFKNINPPTMEGLGVLMAKKKH 120

Query: 372  ATKTSELELNSSPVQPXXXXXXXXXXXXXXXXXXXXXXXIIDGLKKLYIQKVKPLEVTYR 551
            ++K+S+  +N SP                          I+DGLK+LYIQK+KPLEV YR
Sbjct: 121  SSKSSDPNMNGSPAADTSLTAHWFSSKSSKKISLSSVTSIVDGLKRLYIQKLKPLEVAYR 180

Query: 552  FNDFVSPLLTSSDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPG-----AHIGPEPTTDR 716
            FNDFVSPLLT+SDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPG     AHIGPEPTTDR
Sbjct: 181  FNDFVSPLLTNSDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPGNMQTGAHIGPEPTTDR 240

Query: 717  FVVVMNGPDERSIPGNTVAVQADMPYSGLTTFGTAFLSKFECSQMPHPLLEHITFVDTPG 896
            FVVVM+GPDERSIPGNTVAVQADMP+SGLTTFGTAFLSKFECSQMPHPLLEH+TFVDTPG
Sbjct: 241  FVVVMSGPDERSIPGNTVAVQADMPFSGLTTFGTAFLSKFECSQMPHPLLEHVTFVDTPG 300

Query: 897  VLSGEKQRTQRSYDFTGVTAWFAAKCDLILLLFDPHKLDISDEFKRVISSLRGHDDKIRV 1076
            VLSGEKQRTQR+YDFTGVT+WFA+KCDLILLLFDPHKLD+SDEFKRVISSLRGHDDKIRV
Sbjct: 301  VLSGEKQRTQRAYDFTGVTSWFASKCDLILLLFDPHKLDVSDEFKRVISSLRGHDDKIRV 360

Query: 1077 VLNKADQVDTQQLMRVYGALMWSLGKVLNTPEVMRVYIGSFNDKPINIATAGPLGQELFE 1256
            VLNKADQVDTQQLMRVYGALMWSLGKVLNTPEV RVYIGSF+DKPIN A  GP+G+ELFE
Sbjct: 361  VLNKADQVDTQQLMRVYGALMWSLGKVLNTPEVSRVYIGSFSDKPINEAATGPIGRELFE 420

Query: 1257 REQEDLLTDLRDIPKKACDRRINEFVKRARAAKIHSYIISHLKKEMPAMMGKAKAQQRLI 1436
            +EQ+DLL DL+DIPKKACDRRINEFVKRARAAKIH+YIISHLKKEMPA+MGKAKAQQ+LI
Sbjct: 421  KEQDDLLADLKDIPKKACDRRINEFVKRARAAKIHAYIISHLKKEMPAIMGKAKAQQKLI 480

Query: 1437 DNLEDEFKKVQRDYHLPAGDFPSVEHFREILNGYNIDKFEKLKPKMIQTVDDMLGYDIPN 1616
            DNLEDEF KVQR++HLP GDFP+V+HFRE+L+GYNIDKFEKLKPKM+QTVDDMLGYDIP 
Sbjct: 481  DNLEDEFGKVQREHHLPKGDFPNVDHFREVLSGYNIDKFEKLKPKMLQTVDDMLGYDIPE 540

Query: 1617 LLKSFSNPY 1643
            LLK+F NPY
Sbjct: 541  LLKNFRNPY 549


>ref|XP_003617631.1| EH-domain-containing protein [Medicago truncatula]
            gi|355518966|gb|AET00590.1| EH-domain-containing protein
            [Medicago truncatula]
          Length = 543

 Score =  880 bits (2275), Expect = 0.0
 Identities = 437/544 (80%), Positives = 474/544 (87%)
 Frame = +3

Query: 12   MEINSSSISRCSKEHHKIYQDWFNFADSDSDGRLTGADAIKFFSMSNLPRPDLKQVWAIA 191
            ME +   I  CSK+H  IYQ WFN ADSD DGR+TG DA KFF+MSNL R  LKQVWAIA
Sbjct: 1    MEFDPIPIGSCSKDHQNIYQQWFNHADSDGDGRITGNDATKFFAMSNLSRQHLKQVWAIA 60

Query: 192  DSKRQGFLGFNEFVTAMQLVSLAQSGQPITSDLLNSEVDFENLNLPTMDGLDSLLAKQKR 371
            DSKRQG+LGF EF+ AMQLVSLAQSG PIT DLLNS+VD +NL  P ++GLD+LLAK+K 
Sbjct: 61   DSKRQGYLGFQEFIIAMQLVSLAQSGHPITHDLLNSDVDLKNLKPPILEGLDALLAKKKH 120

Query: 372  ATKTSELELNSSPVQPXXXXXXXXXXXXXXXXXXXXXXXIIDGLKKLYIQKVKPLEVTYR 551
              K SEL++N S                           IIDGLK+LYIQK+KPLEVTYR
Sbjct: 121  --KQSELDVNGSSQLQPSPSSSWFSSKSTKKVPLSSVTSIIDGLKRLYIQKLKPLEVTYR 178

Query: 552  FNDFVSPLLTSSDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPGAHIGPEPTTDRFVVVM 731
            FNDFVSPLLT+SDFDAKPMVMLLGQYSTGKTTFIKHLLK SYPGAHIGPEPTTDRFVVVM
Sbjct: 179  FNDFVSPLLTNSDFDAKPMVMLLGQYSTGKTTFIKHLLKCSYPGAHIGPEPTTDRFVVVM 238

Query: 732  NGPDERSIPGNTVAVQADMPYSGLTTFGTAFLSKFECSQMPHPLLEHITFVDTPGVLSGE 911
            +GPDERSIPGNTVAVQADMP+SGLTTFGTAFLSKFECSQMPHPLLEHITFVD+PGVLSGE
Sbjct: 239  SGPDERSIPGNTVAVQADMPFSGLTTFGTAFLSKFECSQMPHPLLEHITFVDSPGVLSGE 298

Query: 912  KQRTQRSYDFTGVTAWFAAKCDLILLLFDPHKLDISDEFKRVISSLRGHDDKIRVVLNKA 1091
            KQRTQR+YDFTGVT+WFAAKCDLILLLFDPHKLDISDEF RVISSLRGHDDKIRVVLNKA
Sbjct: 299  KQRTQRAYDFTGVTSWFAAKCDLILLLFDPHKLDISDEFNRVISSLRGHDDKIRVVLNKA 358

Query: 1092 DQVDTQQLMRVYGALMWSLGKVLNTPEVMRVYIGSFNDKPINIATAGPLGQELFEREQED 1271
            DQVDTQQLMRVYGALMWSLGKVLNTPEV RVYIGSFNDKP+N A +GP+G+ELFE+EQED
Sbjct: 359  DQVDTQQLMRVYGALMWSLGKVLNTPEVTRVYIGSFNDKPVNDAVSGPIGKELFEKEQED 418

Query: 1272 LLTDLRDIPKKACDRRINEFVKRARAAKIHSYIISHLKKEMPAMMGKAKAQQRLIDNLED 1451
            LL+DL+DIPK ACDRRINEFVKRARAAKIH+YIISHLKKEMPAMMGKAKAQQ+LIDNL  
Sbjct: 419  LLSDLKDIPKAACDRRINEFVKRARAAKIHAYIISHLKKEMPAMMGKAKAQQKLIDNLAG 478

Query: 1452 EFKKVQRDYHLPAGDFPSVEHFREILNGYNIDKFEKLKPKMIQTVDDMLGYDIPNLLKSF 1631
            EF KVQR++HLP GDFP+VEHFRE L+GYNIDKFEKLKPKM+Q VDDML YDIPNLLK+F
Sbjct: 479  EFGKVQREFHLPPGDFPNVEHFRESLSGYNIDKFEKLKPKMLQVVDDMLAYDIPNLLKNF 538

Query: 1632 SNPY 1643
             NPY
Sbjct: 539  KNPY 542


>ref|XP_004149345.1| PREDICTED: EH domain-containing protein 1-like [Cucumis sativus]
            gi|449521467|ref|XP_004167751.1| PREDICTED: EH
            domain-containing protein 1-like [Cucumis sativus]
          Length = 547

 Score =  879 bits (2270), Expect = 0.0
 Identities = 432/546 (79%), Positives = 480/546 (87%), Gaps = 2/546 (0%)
 Frame = +3

Query: 12   MEINSSSISRCSKEHHKIYQDWFNFADSDSDGRLTGADAIKFFSMSNLPRPDLKQVWAIA 191
            MEI S  I  CS++H KIYQ+WF++ADSD DGR+TG DAIKFFSMS LPR DLKQVWAIA
Sbjct: 1    MEIGSYPIGSCSRDHQKIYQEWFDYADSDGDGRITGNDAIKFFSMSTLPRQDLKQVWAIA 60

Query: 192  DSKRQGFLGFNEFVTAMQLVSLAQSGQPITSDLL--NSEVDFENLNLPTMDGLDSLLAKQ 365
            DSKRQG+LGF EFVTAMQLVSLAQSG  +T D+L  NSEVD ++LN P M+GLD +LAK+
Sbjct: 61   DSKRQGYLGFKEFVTAMQLVSLAQSGGEVTHDVLTSNSEVDLKSLNPPRMEGLDVILAKK 120

Query: 366  KRATKTSELELNSSPVQPXXXXXXXXXXXXXXXXXXXXXXXIIDGLKKLYIQKVKPLEVT 545
             R  K+++ E N S                           I+DGLK+LYIQK+KPLEV+
Sbjct: 121  ARKQKSNDHETNGSSKGQSPVSASWFSSKSSKKIPLSSVTSIVDGLKRLYIQKLKPLEVS 180

Query: 546  YRFNDFVSPLLTSSDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPGAHIGPEPTTDRFVV 725
            YRFNDFVSPLL +SDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPGAHIGPEPTTDRFVV
Sbjct: 181  YRFNDFVSPLLANSDFDAKPMVMLLGQYSTGKTTFIKHLLKSSYPGAHIGPEPTTDRFVV 240

Query: 726  VMNGPDERSIPGNTVAVQADMPYSGLTTFGTAFLSKFECSQMPHPLLEHITFVDTPGVLS 905
            VM+GPDERSIPGNT+AVQADMP++GLTTFGT+FLSKFECSQMPHPLLEHITFVDTPGVLS
Sbjct: 241  VMSGPDERSIPGNTIAVQADMPFNGLTTFGTSFLSKFECSQMPHPLLEHITFVDTPGVLS 300

Query: 906  GEKQRTQRSYDFTGVTAWFAAKCDLILLLFDPHKLDISDEFKRVISSLRGHDDKIRVVLN 1085
            GEKQRTQR+Y+FT VT+WFAAKCDLILLLFDPHKLDISDEFKRVI+SLRGHDDKIRVVLN
Sbjct: 301  GEKQRTQRAYEFTSVTSWFAAKCDLILLLFDPHKLDISDEFKRVIASLRGHDDKIRVVLN 360

Query: 1086 KADQVDTQQLMRVYGALMWSLGKVLNTPEVMRVYIGSFNDKPINIATAGPLGQELFEREQ 1265
            KADQVDTQQLMRVYGALMWSLGKVLNTPEV+RVYIGSFNDKP+N A  GPLG++LFE+EQ
Sbjct: 361  KADQVDTQQLMRVYGALMWSLGKVLNTPEVVRVYIGSFNDKPVNEAVTGPLGKDLFEKEQ 420

Query: 1266 EDLLTDLRDIPKKACDRRINEFVKRARAAKIHSYIISHLKKEMPAMMGKAKAQQRLIDNL 1445
            EDLL+DL+DIPKKACDRRINEFVKRARAAKIH+YIISHL+KEMP M+GKAK QQRLIDNL
Sbjct: 421  EDLLSDLKDIPKKACDRRINEFVKRARAAKIHAYIISHLRKEMPTMIGKAKTQQRLIDNL 480

Query: 1446 EDEFKKVQRDYHLPAGDFPSVEHFREILNGYNIDKFEKLKPKMIQTVDDMLGYDIPNLLK 1625
             DEF KVQR+YHLPAGDFP+VE FRE L+GYN DKFEKLKPKMIQ+VDDMLGYD+P+LLK
Sbjct: 481  ADEFGKVQREYHLPAGDFPNVEQFRETLSGYNFDKFEKLKPKMIQSVDDMLGYDVPDLLK 540

Query: 1626 SFSNPY 1643
            +F NPY
Sbjct: 541  NFRNPY 546