BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Angelica22_contig00016447
         (2199 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI16805.3| unnamed protein product [Vitis vinifera]              390   e-105
ref|NP_172762.2| coilin [Arabidopsis thaliana] gi|20260258|gb|AA...   338   5e-90
ref|XP_002892725.1| hypothetical protein ARALYDRAFT_471456 [Arab...   337   6e-90
ref|XP_002530050.1| conserved hypothetical protein [Ricinus comm...   313   1e-82
gb|AAD31056.1|AC007357_5 F3F19.5 [Arabidopsis thaliana]               274   6e-71

>emb|CBI16805.3| unnamed protein product [Vitis vinifera]
          Length = 652

 Score =  390 bits (1001), Expect = e-105
 Identities = 262/672 (38%), Positives = 359/672 (53%), Gaps = 66/672 (9%)
 Frame = +3

Query: 132  LRVRLVFEDEHILSKSQRSQGLQQSWLLLKPNQHPNFFQLSHHVLHLFGLNQSCPNGLVL 311
            +RVR+V ED  +L+K+Q S+GL++SWLLLKP QH     LS ++L +F L+  CPNGL+L
Sbjct: 6    VRVRVVLEDPDLLNKTQNSEGLRRSWLLLKP-QHKTISDLSSYLLRIFNLHDFCPNGLLL 64

Query: 312  SMDGFVLPPFESTCFLKDKEIIXXXXXXXXXXXXXXXTDKNGPAEEQGIV------KGVQ 473
            SMDGFVLP FESTC LKDKEII                D+   +E++ IV      +GV+
Sbjct: 65   SMDGFVLPSFESTCILKDKEIISVKRKGGAVIDLLEVGDETNCSEDEAIVENQHIHRGVK 124

Query: 474  LLANEEFNKESGGYQXXXXXXXXXXX-----IENTSGGNALSKKRKASERLAGSKKKKHK 638
            LLANEEF+KE+GGY+                +E  S GNA SKKRKAS +L   K+KK+K
Sbjct: 125  LLANEEFDKETGGYESESEEDEPDQPEETVQVETASAGNAGSKKRKASRKLKSPKRKKNK 184

Query: 639  ST-------VPDGAXXXXXXXXXXXXHGDGVLVGKSRNRKENSSNTKTKPXXXXXXXXXX 797
             T       V +                   L  K   +K  SSN   KP          
Sbjct: 185  YTRLEKCPVVLEDVENGVCEEQTKSCDDCTALPKKGSLKKHKSSNVNGKPDKARTLNIDE 244

Query: 798  XXXXXXXXXXXXXX--QRKENDEEKEETSNAP--TKKLPXXXXXXXXXXXQWLRAMAKIG 965
                            + +EN  +  E +N P  T+K P           +WLR +AK+ 
Sbjct: 245  RSNDVDESSPNAKRCGELQENGSQGVEVANPPDGTQKYPSRSARRKKAKRKWLRELAKVE 304

Query: 966  KEEVCDTKRPLKQKERRPRAEKKEVICQSKGLLHWKQSREGYHKYKKEDSA--PVSTRPG 1139
            K+E+   + P           +KEV  Q   L H +  +        +D+   P+  RPG
Sbjct: 305  KKEMHQRQSP-----------EKEV--QKNSLEHQQPDQNS----DTDDAVIVPIVIRPG 347

Query: 1140 HIRFEPRDEDEAVRESQVSVETFQWNGITSKKQGQKWGTEKCSSNQRNDFVNMNGDHSDT 1319
            HIRFEP  +D+ ++++ VSVETFQWNG TSKK+GQKWG EK S  +RND+ + N  HS+T
Sbjct: 348  HIRFEPLGKDQTIQQNPVSVETFQWNGTTSKKKGQKWGKEKMSC-RRNDYKDFNQQHSET 406

Query: 1320 HNIGKDIVISSAIEFNELPPLPGNMPKEGYVIAYRLLELSSSWTPEPSEFRVGRVLWYKP 1499
              + +       ++F++LP L  + PKEG +IAYRL+ELSS+WTPE S FRVG++  Y P
Sbjct: 407  FAVEEGTPPKDPMDFDKLPSLTSS-PKEGDMIAYRLIELSSTWTPELSTFRVGKISSYDP 465

Query: 1500 ESRTIMLAPVPEYPVVLE-KLDGEESASQ--QDISLYKEDGSLEIDFRSLIDVRL----- 1655
            ES  ++L  VPE P+V E ++D + SA +   D SLY+EDGSLEIDF SLIDVR+     
Sbjct: 466  ESNKLILISVPESPIVAETRIDEDASALEPDPDTSLYREDGSLEIDFSSLIDVRIIKSGN 525

Query: 1656 -----------------------VKLNDTNSGKDATVGPVGNENATSIL------SDDKQ 1748
                                   VK N+ NSG   ++ P G  N T +       + +++
Sbjct: 526  SHLEKAVTARVEAPVDTQDAVSGVKPNNKNSGMSTSL-PGGELNITQVSVAGVEHNINRE 584

Query: 1749 KQTPDTGNGEVNLWDHFTKDLNTKKAELSEDNSWSTWSEGASKKSAP-----SYRAWRGS 1913
               P   NG+VN WD   K L+ KKA+LS++       +G+SKK +P     SY+A RGS
Sbjct: 585  MTAPPPENGKVNAWDEIDKVLSAKKAQLSQE-------DGSSKKESPGRSPWSYKALRGS 637

Query: 1914 ALGPTMARLRSK 1949
            ALGPTM+ LR++
Sbjct: 638  ALGPTMSFLRAQ 649


>ref|NP_172762.2| coilin [Arabidopsis thaliana] gi|20260258|gb|AAM13027.1| unknown
            protein [Arabidopsis thaliana] gi|22136510|gb|AAM91333.1|
            unknown protein [Arabidopsis thaliana]
            gi|332190840|gb|AEE28961.1| coilin [Arabidopsis thaliana]
          Length = 608

 Score =  338 bits (866), Expect = 5e-90
 Identities = 225/638 (35%), Positives = 333/638 (52%), Gaps = 28/638 (4%)
 Frame = +3

Query: 123  DSNLRVRLVFEDEHILSKSQRSQGLQQSWLLLKPNQHPNFFQLSHHVLHLFGLNQSCPNG 302
            +  +RVRLVFED  ILSK Q+ QGL +SW++L    H    + S H+ H F L ++CP+G
Sbjct: 3    EEKVRVRLVFEDRRILSKYQKKQGLTRSWVVLNRKCHRTISEFSDHIFHTFSLCEACPHG 62

Query: 303  LVLSMDGFVLPPFESTCFLKDKEIIXXXXXXXXXXXXXXX-TDKN-----GPAEEQGIVK 464
            L LSM+GFVLPPFES+C LKDK+I+                +D+N        E   I  
Sbjct: 63   LSLSMEGFVLPPFESSCVLKDKDIVCVKKKKESLLEIVGEDSDENVYNAIEVEERPQIRP 122

Query: 465  GVQLLANEEFNKESGGYQXXXXXXXXXXXIENTSGGNALSKKRKASERLAGSKKKKHKST 644
            G  LLANEEF KE+GGY+            E        SKKRK S +   +K+KK K  
Sbjct: 123  GEMLLANEEFQKETGGYESESEEDELEEEAEEFVPEKKASKKRKTSSKNQSTKRKKCKLD 182

Query: 645  VPDGAXXXXXXXXXXXXHGDGVLVGKSRNRK---------ENSSNTKTKPXXXXXXXXXX 797
              + +                 +V K + +K         +  +N  TKP          
Sbjct: 183  TTEESPDERENTAVVSN-----VVKKKKKKKSLDVQSANNDEQNNDSTKPMTKS------ 231

Query: 798  XXXXXXXXXXXXXXQRKENDEEKEETSN-----APTKKLPXXXXXXXXXXXQWLRAMAKI 962
                          +R    EE +E ++     A TKK P           QWLR   K+
Sbjct: 232  --------------KRSSQQEESKEHNDLCQLSAETKKTPSRSARRKKAKRQWLREKTKL 277

Query: 963  GKEEVCDTKRPLKQKERR----PRAEKKEVICQSKGLLHWKQSREGYHKYKKEDSAPVST 1130
             KEE+  T+  +   ++          KE  C++      ++  +G+     ++  PV  
Sbjct: 278  EKEELLQTQLVVAPSQKPVITIDHQATKEKHCETLENQQAEEVSDGFG----DEVVPVEV 333

Query: 1131 RPGHIRFEP-RDEDEAVRESQVSVETFQWNGITSKKQGQKWGTEKCSSNQRNDFVNMNGD 1307
            RPGHIRF+P    DEA  +S+  VE   WNG  +KK+GQKWGTEK   ++R  +     +
Sbjct: 334  RPGHIRFKPLAGTDEASLDSEPLVENVLWNGNMTKKKGQKWGTEKSGFSKR--YAQDFNE 391

Query: 1308 HSDTHNIGKDIVISSAIEFNELPPLPGNMPKEGYVIAYRLLELSSSWTPEPSEFRVGRVL 1487
             + T     + + +  I++ +L    G++ K+G VIAYRL+EL+SSWTPE S FRVG++ 
Sbjct: 392  DATTQPAEAETLANCPIDYEQLVAYTGSV-KKGDVIAYRLIELTSSWTPEVSSFRVGKIS 450

Query: 1488 WYKPESRTIMLAPVPEYPVVLEKLDGEESASQQDISLYKEDGSLEIDFRSLIDVRLVKLN 1667
            +Y P+S+ + L PV E+P+  +  + ++   Q D SLYKEDGSLEI+F +L+DVR VK +
Sbjct: 451  YYDPDSKMVTLMPVQEFPIEKKTEEDDDFCMQPDTSLYKEDGSLEIEFSALLDVRSVKTS 510

Query: 1668 DTNSGKDA-TVGPVGNENATS-ILSDDKQKQTPDTGNGEVNLWDHFTKDLNTKKAELSE- 1838
             ++S + A +  P  +++A    LS +K+ QTP   NGEV+ W+  ++ L+ KKA LS+ 
Sbjct: 511  SSDSAEVAKSALPEPDQSAKKPKLSANKELQTPAKENGEVSPWEELSEALSAKKAALSQA 570

Query: 1839 DNSWSTWSEGASKKSAPSYRAWRGSALGPTMARLRSKQ 1952
            +N W+   +G+S   + SY+A RGSA+GP M  LRS++
Sbjct: 571  NNGWN--KKGSSSGGSWSYKALRGSAMGPVMNYLRSQK 606


>ref|XP_002892725.1| hypothetical protein ARALYDRAFT_471456 [Arabidopsis lyrata subsp.
            lyrata] gi|297338567|gb|EFH68984.1| hypothetical protein
            ARALYDRAFT_471456 [Arabidopsis lyrata subsp. lyrata]
          Length = 605

 Score =  337 bits (865), Expect = 6e-90
 Identities = 227/634 (35%), Positives = 326/634 (51%), Gaps = 24/634 (3%)
 Frame = +3

Query: 123  DSNLRVRLVFEDEHILSKSQRSQGLQQSWLLLKPNQHPNFFQLSHHVLHLFGLNQSCPNG 302
            +  +R+RLVFED  ILSK Q+ QGL +SW++L   +H    + S H+   F L ++CP G
Sbjct: 3    EEKVRIRLVFEDRRILSKYQKKQGLTRSWVVLNRKRHRTVSEFSDHLFRTFSLCEACPLG 62

Query: 303  LVLSMDGFVLPPFESTCFLKDKEIIXXXXXXXXXXXXXXXTDKNGP------AEEQGIVK 464
            L LSMDGFVLPPFES+C LKDK+I+                 +          E      
Sbjct: 63   LTLSMDGFVLPPFESSCVLKDKDIVRVKKKKESLLEIVGEDSEENVYNAIEVEERPQFRP 122

Query: 465  GVQLLANEEFNKESGGYQXXXXXXXXXXXIENTSGGNALSKKRKASERLAGSKKKKHKST 644
            G  LLANEEF  E+GGY+            E        SKKRKAS +   SK+KK K  
Sbjct: 123  GEMLLANEEFQNETGGYESESEEDEVEEEAEEFVPEKKTSKKRKASSKSLSSKRKKCKLA 182

Query: 645  VPDGAXXXXXXXXXXXXHGDGVLVGKSRNRKENSSNTKTKPXXXXXXXXXXXXXXXXXXX 824
              + +                + V ++ N ++++ NTK                      
Sbjct: 183  TTEESPEERENTAVVKKKKKSLDVQRAENDEQDNGNTKP--------------------- 221

Query: 825  XXXXXQRKENDEEKEETSN-----APTKKLPXXXXXXXXXXXQWLRAMAKIGKEEVCDTK 989
                 +R    EE +E ++       TKK P           QWLR   K+ KEE     
Sbjct: 222  -ITKSKRSSQQEESKEPNDLCQQSTETKKTPSRSARRKKAKRQWLREKTKLEKEE----- 275

Query: 990  RPLKQKERRPRAEKKEVIC---QSKGLLHWK----QSREGYHKYKKEDSAPVSTRPGHIR 1148
              L+QK+      +K VI    Q+    H +    Q  +       ++  PV  RPGHIR
Sbjct: 276  --LQQKQLVVAPSQKPVITIDYQATEENHCEALENQQPDDLSDGVGDEVVPVEVRPGHIR 333

Query: 1149 FEP-RDEDEAVRESQVSVETFQWNGITSKKQGQKWGTEKCSSNQR--NDFVNMNGDHS-D 1316
            F+P    DEA  ES+  VE F WNG  +KK+GQKWGTEK   ++R   DF   N D +  
Sbjct: 334  FKPLTGTDEAPLESEPLVEKFLWNGNMTKKKGQKWGTEKSGFSKRYAQDF---NEDTTYQ 390

Query: 1317 THNIGKDIVISSAIEFNELPPLPGNMPKEGYVIAYRLLELSSSWTPEPSEFRVGRVLWYK 1496
            T     +      I++ +L    G++ K+G VIAYRL+EL+SSWTPE S FRVG++ +Y 
Sbjct: 391  TQPTEAETPAKGPIDYEQLVAYTGSV-KKGDVIAYRLIELTSSWTPEVSSFRVGKISYYD 449

Query: 1497 PESRTIMLAPVPEYPVVLEKLDGEESASQQDISLYKEDGSLEIDFRSLIDVRLVKLNDTN 1676
            P+S+ + L PV E+P+  +  + ++ + + D +LYKEDGSLEI+F +L+DVR VK + ++
Sbjct: 450  PDSKKVTLMPVQEFPIEKKTEEDDDFSMEPDTALYKEDGSLEIEFSALLDVRSVKTSSSD 509

Query: 1677 SGKDA-TVGPVGNENATSI-LSDDKQKQTPDTGNGEVNLWDHFTKDLNTKKAELSEDNSW 1850
            S + A +  P  +++AT + LS +K  QTP   NG+VN W+  ++ L+ KKAELS+ N+ 
Sbjct: 510  SAEVAKSAPPEPDQSATKLKLSANKDLQTPIKENGKVNPWEELSEALSAKKAELSQANNG 569

Query: 1851 STWSEGASKKSAPSYRAWRGSALGPTMARLRSKQ 1952
                  +S   + SY+A RGSA+GP M  LRS++
Sbjct: 570  WNKKGSSSGGGSWSYKALRGSAMGPVMNYLRSQK 603


>ref|XP_002530050.1| conserved hypothetical protein [Ricinus communis]
            gi|223530466|gb|EEF32350.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 607

 Score =  313 bits (802), Expect = 1e-82
 Identities = 226/633 (35%), Positives = 323/633 (51%), Gaps = 27/633 (4%)
 Frame = +3

Query: 132  LRVRLVFEDEHILSKSQRSQGLQQSWLLLKPNQHPNFFQLSHHVLHLFGLNQSCPNGLVL 311
            +R+RLVF+   ILSK Q +QGL++ W+LLKP QH     LS ++L++F L   CP+GL+L
Sbjct: 4    VRLRLVFDQ--ILSKVQNTQGLKRCWILLKP-QHQTISDLSSYLLNVFNLQNHCPHGLLL 60

Query: 312  SMDGFVLPPFESTCFLKDKEIIXXXXXXXXXXXXXXXTDK-NGPAEEQGIVK-------G 467
             M+GF LP FE T  LKDK+II                D  N   E   IV+       G
Sbjct: 61   LMEGFALPGFECTSILKDKDIIRVEKNGGVSSEIVMVGDDVNNALEVVEIVETQPLVTTG 120

Query: 468  VQLLANEEFNKESGGYQXXXXXXXXXXX------IENTSGGNALSKKRKASERLAGSKKK 629
            + LLANEEF KESGGYQ                 +EN S    +SKKRKA + L   K+K
Sbjct: 121  MNLLANEEFEKESGGYQSDEEEDVPKQEEEAVHVVENASEVKTISKKRKAMKDLKSPKRK 180

Query: 630  KHKSTVPDG-AXXXXXXXXXXXXHGDGVLVGKSRNRKENSSNTKTKPXXXXXXXXXXXXX 806
            K KS   +  A              +G       + +  SS   +K              
Sbjct: 181  KTKSARAEKHAAVLEDMGNNVTGEQNGTSCTPKFDERSESSKALSKAKRIS--------- 231

Query: 807  XXXXXXXXXXXQRKENDEEKEETS--NAPTKKLPXXXXXXXXXXXQWLRAMAKIGKEEVC 980
                       Q +EN +   + S   + TKKLP           +WLR   +  ++E  
Sbjct: 232  -----------QPQENGDASVDASPSTSGTKKLPSRSARRKQAKRRWLREKLRAERKE-- 278

Query: 981  DTKRPLKQKERRPRAEKKEVICQSKGLLHWKQSREGYHKYKKEDS-APVSTRPGHIRFEP 1157
             + + L +K      E+   + +S G    +  ++       ED   P+  RPGHIRFEP
Sbjct: 279  QSSQTLAEKGNNGVFEE---LPESGGEEPQEDDQQPDKDSDLEDDIVPIVIRPGHIRFEP 335

Query: 1158 RDE---DEAVRESQVSVETFQWNGITSKKQGQKWGTEKCSSNQRNDFVNMNGDHSDTHNI 1328
              +   ++A +++ + VE F WNGITSKK+GQKWG EK +S +RND  N++ + S+    
Sbjct: 336  LSKAGANQAEQQTHIPVEIFHWNGITSKKKGQKWGKEKATSYKRNDHRNVSQECSEVRKD 395

Query: 1329 GKDIVISSAIEFNELPPLPGNMPKEGYVIAYRLLELSSSWTPEPSEFRVGRVLWYKPESR 1508
            G   V    ++F +L      +PKEG VIAYRL+ELS SWTPE S +RVG++  Y  +S 
Sbjct: 396  GGRPVYDR-VDFEKLE-FYTTLPKEGDVIAYRLIELSPSWTPEISSYRVGKISRYDMQSN 453

Query: 1509 TIMLAPVPEYPVVLEKLDGEESASQQDISLYKEDGSLEIDFRSLIDVRLVKLNDTNSGKD 1688
             + L PVP YPV  ++   + SA+  +   Y +DGSL I+F SLI+VRLV   ++NS K 
Sbjct: 454  RVRLVPVPGYPVTHKETGDDASAAPSETIPYAKDGSLWIEFASLIEVRLVTRGNSNSVKS 513

Query: 1689 AT-----VGPVGNENATSILSDD-KQKQTPDTGNGEVNLWDHFTKDLNTKKAELSEDNSW 1850
                   V     +N T   S++  +       NG+ N W+  ++ LN KKAEL+++++W
Sbjct: 514  VAGEIDKVPVRDQDNRTGCRSNNGNESHVSAQENGKGNAWEEISEALNAKKAELAQEDNW 573

Query: 1851 STWSEGASKKSAPSYRAWRGSALGPTMARLRSK 1949
            +    G+S +   SY+A RGSALGPTMA LR++
Sbjct: 574  N--KPGSSGRRPWSYKALRGSALGPTMALLRAQ 604


>gb|AAD31056.1|AC007357_5 F3F19.5 [Arabidopsis thaliana]
          Length = 547

 Score =  274 bits (701), Expect = 6e-71
 Identities = 194/574 (33%), Positives = 291/574 (50%), Gaps = 28/574 (4%)
 Frame = +3

Query: 315  MDGFVLPPFESTCFLKDKEIIXXXXXXXXXXXXXXX-TDKN-----GPAEEQGIVKGVQL 476
            M+GFVLPPFES+C LKDK+I+                +D+N        E   I  G  L
Sbjct: 6    MEGFVLPPFESSCVLKDKDIVCVKKKKESLLEIVGEDSDENVYNAIEVEERPQIRPGEML 65

Query: 477  LANEEFNKESGGYQXXXXXXXXXXXIENTSGGNALSKKRKASERLAGSKKKKHKSTVPDG 656
            LANEEF KE+GGY+            E        SKKRK S +   +K+KK K    + 
Sbjct: 66   LANEEFQKETGGYESESEEDELEEEAEEFVPEKKASKKRKTSSKNQSTKRKKCKLDTTEE 125

Query: 657  AXXXXXXXXXXXXHGDGVLVGKSRNRK---------ENSSNTKTKPXXXXXXXXXXXXXX 809
            +                 +V K + +K         +  +N  TKP              
Sbjct: 126  SPDERENTAVVSN-----VVKKKKKKKSLDVQSANNDEQNNDSTKPMTKS---------- 170

Query: 810  XXXXXXXXXXQRKENDEEKEETSN-----APTKKLPXXXXXXXXXXXQWLRAMAKIGKEE 974
                      +R    EE +E ++     A TKK P           QWLR   K+ KEE
Sbjct: 171  ----------KRSSQQEESKEHNDLCQLSAETKKTPSRSARRKKAKRQWLREKTKLEKEE 220

Query: 975  VCDTKRPLKQKERR----PRAEKKEVICQSKGLLHWKQSREGYHKYKKEDSAPVSTRPGH 1142
            +  T+  +   ++          KE  C++      ++  +G+     ++  PV  RPGH
Sbjct: 221  LLQTQLVVAPSQKPVITIDHQATKEKHCETLENQQAEEVSDGFG----DEVVPVEVRPGH 276

Query: 1143 IRFEP-RDEDEAVRESQVSVETFQWNGITSKKQGQKWGTEKCSSNQRNDFVNMNGDHSDT 1319
            IRF+P    DEA  +S+  VE   WNG  +KK+GQKWGTEK   ++R  +     + + T
Sbjct: 277  IRFKPLAGTDEASLDSEPLVENVLWNGNMTKKKGQKWGTEKSGFSKR--YAQDFNEDATT 334

Query: 1320 HNIGKDIVISSAIEFNELPPLPGNMPKEGYVIAYRLLELSSSWTPEPSEFRVGRVLWYKP 1499
                 + + +  I++ +L    G++ K+G VIAYRL+EL+SSWTPE S FRVG++ +Y P
Sbjct: 335  QPAEAETLANCPIDYEQLVAYTGSV-KKGDVIAYRLIELTSSWTPEVSSFRVGKISYYDP 393

Query: 1500 ESRTIMLAPVPEYPVVLEKLDGEESASQQDISLYKEDGSLEIDFRSLIDVRLVKLNDTNS 1679
            +S+ + L PV E+P+  +  + ++   Q D SLYKEDGSLEI+F +L+DVR VK + ++S
Sbjct: 394  DSKMVTLMPVQEFPIEKKTEEDDDFCMQPDTSLYKEDGSLEIEFSALLDVRSVKTSSSDS 453

Query: 1680 GKDA-TVGPVGNENATS-ILSDDKQKQTPDTGNGEVNLWDHFTKDLNTKKAELSE-DNSW 1850
             + A +  P  +++A    LS +K+ QTP   NGEV+ W+  ++ L+ KKA LS+ +N W
Sbjct: 454  AEVAKSALPEPDQSAKKPKLSANKELQTPAKENGEVSPWEELSEALSAKKAALSQANNGW 513

Query: 1851 STWSEGASKKSAPSYRAWRGSALGPTMARLRSKQ 1952
            +   +G+S   + SY+A RGSA+GP M  LRS++
Sbjct: 514  N--KKGSSSGGSWSYKALRGSAMGPVMNYLRSQK 545