BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Angelica22_contig00010514
         (1805 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]    624   0.0  
gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]        620   0.0  
sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desatur...   619   0.0  
ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vi...   555   e-177
ref|XP_002302613.1| predicted protein [Populus trichocarpa] gi|2...   557   e-175

>gb|ADK91077.1| delta-8 sphingolipid desaturase [Helianthus annuus]
          Length = 466

 Score =  624 bits (1608), Expect(2) = 0.0
 Identities = 286/349 (81%), Positives = 313/349 (89%)
 Frame = +2

Query: 236  KKYITSEELGKHNKREDLWISIQGKVYNVTEWAKEHPGGDIPLLNMAGQDVTDAFIAFHP 415
            KKYITS+EL KHNK  DLWISIQGKVYNVTEWAK HPGG+IPL+N+AGQDVTDAFIAFHP
Sbjct: 24   KKYITSDELKKHNKPNDLWISIQGKVYNVTEWAKIHPGGEIPLMNLAGQDVTDAFIAFHP 83

Query: 416  GSAWQHLDKFFTGYHLIDYKVSDVSRDYRKLAAEFAKAGMFEKKGHIVIYSICFVALLLS 595
            GSAW+HL K FTGYHL DY+VS VS+DYRKLA+EFAKAGMFEKKGH VIYS+CFV+LLLS
Sbjct: 84   GSAWEHLSKLFTGYHLKDYQVSAVSKDYRKLASEFAKAGMFEKKGHGVIYSLCFVSLLLS 143

Query: 596  ACIYGVLSSDSFAVHMLSGVLLGFTWIQVAYMGHDAGHYQAMSSRGWNKVAGILIGNCIT 775
             C+YGVL S SF VHML+G LLG  WIQ+AY+GHDAGHYQ MS+RGWNK AGI IGNCIT
Sbjct: 144  GCVYGVLFSSSFWVHMLAGALLGLAWIQIAYLGHDAGHYQMMSTRGWNKFAGIFIGNCIT 203

Query: 776  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSDSFFQSLTSKFYNRKLTFDPVAKF 955
            GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS   F SLTS FY R+LTFD +A+F
Sbjct: 204  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSRLFNSLTSVFYGRQLTFDRLARF 263

Query: 956  FVSYQHLSYYPVMCVARVNLYLQTLLLLCSKRKIPDRAFNILGTIIFWTWFPLLVSCLPN 1135
            FVSYQH SYYP+MCVARVNLYLQTLLLL SKRK+PD+  NI GT++FWTWFPLLVSCLPN
Sbjct: 264  FVSYQHYSYYPIMCVARVNLYLQTLLLLLSKRKVPDKGLNIFGTLVFWTWFPLLVSCLPN 323

Query: 1136 WTERVLFVLISFCVCAIQHVQFTLNHFAADVYVGPPTGNNWFEKQSGGT 1282
            W ERV FVL+SFCV  IQHVQFTLNHFAA+VYVGPPTGN+WFEKQ+ GT
Sbjct: 324  WPERVAFVLVSFCVTGIQHVQFTLNHFAANVYVGPPTGNDWFEKQTNGT 372



 Score = 91.7 bits (226), Expect(2) = 0.0
 Identities = 45/68 (66%), Positives = 53/68 (77%), Gaps = 8/68 (11%)
 Frame = +1

Query: 1381 RLPRTHLRSISPIVQDLCKKHNLTYRSLSFYEANVATLKTLKTAAYQAR--------GLL 1536
            RLPR HLRSI+PIV++LCKKHNL Y S+SF++AN+ TLKTL+TAA QAR         LL
Sbjct: 399  RLPRCHLRSIAPIVRELCKKHNLPYTSVSFFDANIMTLKTLRTAALQARDLTNPAPQNLL 458

Query: 1537 WEAVNTHG 1560
            WEA NTHG
Sbjct: 459  WEAFNTHG 466


>gb|ABA55805.1| delta-8 sphingolipid desaturase [Crepis alpina]
          Length = 469

 Score =  620 bits (1598), Expect(2) = 0.0
 Identities = 279/349 (79%), Positives = 311/349 (89%)
 Frame = +2

Query: 236  KKYITSEELGKHNKREDLWISIQGKVYNVTEWAKEHPGGDIPLLNMAGQDVTDAFIAFHP 415
            KKYITSEEL KHNK  DLWISIQGKVYNVTEW+K HPGGDIPLLN+AGQDVTDAFIAFHP
Sbjct: 27   KKYITSEELEKHNKPTDLWISIQGKVYNVTEWSKIHPGGDIPLLNLAGQDVTDAFIAFHP 86

Query: 416  GSAWQHLDKFFTGYHLIDYKVSDVSRDYRKLAAEFAKAGMFEKKGHIVIYSICFVALLLS 595
            GSAW HL   FTGYHL DY+VSD+S+DYRKLA+EF+KAGMF+KKGH VIYS+CFV++LLS
Sbjct: 87   GSAWHHLQSLFTGYHLQDYQVSDISKDYRKLASEFSKAGMFDKKGHGVIYSLCFVSILLS 146

Query: 596  ACIYGVLSSDSFAVHMLSGVLLGFTWIQVAYMGHDAGHYQAMSSRGWNKVAGILIGNCIT 775
             C+YGVL S S  VHM +G LLG  W+Q+AY+GHDAGHYQ M++RGWNK AGI IGNCIT
Sbjct: 147  LCVYGVLCSASVTVHMFAGALLGLAWMQIAYLGHDAGHYQMMATRGWNKFAGIFIGNCIT 206

Query: 776  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSDSFFQSLTSKFYNRKLTFDPVAKF 955
            GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS   F+S+TS FY R+LTFD VA+F
Sbjct: 207  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFESITSVFYGRRLTFDSVARF 266

Query: 956  FVSYQHLSYYPVMCVARVNLYLQTLLLLCSKRKIPDRAFNILGTIIFWTWFPLLVSCLPN 1135
            F+SYQH +YYP+MCV RVNLYLQT+LLLCSKR+IPDR  NI+GT+IFWTWFPLLVSCLPN
Sbjct: 267  FISYQHYTYYPIMCVTRVNLYLQTILLLCSKRRIPDRTLNIIGTLIFWTWFPLLVSCLPN 326

Query: 1136 WTERVLFVLISFCVCAIQHVQFTLNHFAADVYVGPPTGNNWFEKQSGGT 1282
            W ERV FVL+SFCV  IQHVQFTLNHFAADVYVGPP GN+WFEKQ+ GT
Sbjct: 327  WPERVAFVLVSFCVTGIQHVQFTLNHFAADVYVGPPRGNDWFEKQTAGT 375



 Score = 94.7 bits (234), Expect(2) = 0.0
 Identities = 48/68 (70%), Positives = 52/68 (76%), Gaps = 8/68 (11%)
 Frame = +1

Query: 1381 RLPRTHLRSISPIVQDLCKKHNLTYRSLSFYEANVATLKTLKTAAYQAR--------GLL 1536
            RLPR HLRSISPIV DLCKKHNL Y SLSF++ANV TLKTL+TAA QAR         L+
Sbjct: 402  RLPRCHLRSISPIVMDLCKKHNLPYTSLSFFDANVTTLKTLRTAALQARDLANGPPQNLV 461

Query: 1537 WEAVNTHG 1560
            WEA NTHG
Sbjct: 462  WEAFNTHG 469


>sp|Q43469.1|SLD1_HELAN RecName: Full=Delta(8)-fatty-acid desaturase; AltName:
            Full=Delta(8)-sphingolipid desaturase
            gi|1040729|emb|CAA60621.1| delta-8 sphingolipid
            desaturase [Helianthus annuus]
          Length = 458

 Score =  619 bits (1597), Expect(2) = 0.0
 Identities = 282/349 (80%), Positives = 312/349 (89%)
 Frame = +2

Query: 236  KKYITSEELGKHNKREDLWISIQGKVYNVTEWAKEHPGGDIPLLNMAGQDVTDAFIAFHP 415
            KKYITS+EL KHN   DLWISI GKVYNVTEWAKEHPGGD PL+N+AGQDVTDAFIAFHP
Sbjct: 16   KKYITSKELKKHNNPNDLWISILGKVYNVTEWAKEHPGGDAPLINLAGQDVTDAFIAFHP 75

Query: 416  GSAWQHLDKFFTGYHLIDYKVSDVSRDYRKLAAEFAKAGMFEKKGHIVIYSICFVALLLS 595
            G+AW+HLDK FTGYHL DY+VSD+SRDYRKLA+EFAKAGMFEKKGH VIYS+CFV+LLLS
Sbjct: 76   GTAWKHLDKLFTGYHLKDYQVSDISRDYRKLASEFAKAGMFEKKGHGVIYSLCFVSLLLS 135

Query: 596  ACIYGVLSSDSFAVHMLSGVLLGFTWIQVAYMGHDAGHYQAMSSRGWNKVAGILIGNCIT 775
            AC+YGVL S SF +HMLSG +LG  W+Q+AY+GHDAGHYQ M++RGWNK AGI IGNCIT
Sbjct: 136  ACVYGVLYSGSFWIHMLSGAILGLAWMQIAYLGHDAGHYQMMATRGWNKFAGIFIGNCIT 195

Query: 776  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSDSFFQSLTSKFYNRKLTFDPVAKF 955
            GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVS   F S+TS FY R+LTFDP+A+F
Sbjct: 196  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNSITSVFYGRQLTFDPLARF 255

Query: 956  FVSYQHLSYYPVMCVARVNLYLQTLLLLCSKRKIPDRAFNILGTIIFWTWFPLLVSCLPN 1135
            FVSYQH  YYP+MCVARVNLYLQT+LLL SKRKIPDR  NILGT+IFWTWFPLLVS LPN
Sbjct: 256  FVSYQHYLYYPIMCVARVNLYLQTILLLISKRKIPDRGLNILGTLIFWTWFPLLVSRLPN 315

Query: 1136 WTERVLFVLISFCVCAIQHVQFTLNHFAADVYVGPPTGNNWFEKQSGGT 1282
            W ERV FVL+SFCV  IQH+QFTLNHF+ DVYVGPP G+NWFEKQ+ GT
Sbjct: 316  WPERVAFVLVSFCVTGIQHIQFTLNHFSGDVYVGPPKGDNWFEKQTRGT 364



 Score = 89.4 bits (220), Expect(2) = 0.0
 Identities = 46/68 (67%), Positives = 51/68 (75%), Gaps = 8/68 (11%)
 Frame = +1

Query: 1381 RLPRTHLRSISPIVQDLCKKHNLTYRSLSFYEANVATLKTLKTAAYQAR--------GLL 1536
            RLPR HLRSISPI ++LCKK+NL Y SLSFY+ANV TLKTL+TAA QAR         L 
Sbjct: 391  RLPRCHLRSISPICRELCKKYNLPYVSLSFYDANVTTLKTLRTAALQARDLTNPAPQNLA 450

Query: 1537 WEAVNTHG 1560
            WEA NTHG
Sbjct: 451  WEAFNTHG 458


>ref|XP_002279227.1| PREDICTED: fatty acid desaturase 3 [Vitis vinifera]
          Length = 447

 Score =  555 bits (1430), Expect(2) = e-177
 Identities = 251/349 (71%), Positives = 298/349 (85%)
 Frame = +2

Query: 236  KKYITSEELGKHNKREDLWISIQGKVYNVTEWAKEHPGGDIPLLNMAGQDVTDAFIAFHP 415
            +KYI++EEL +H+K  DLWISIQGKVYNVT+WAK HPGGDIPLLN+AGQDVTDAFIA+HP
Sbjct: 5    RKYISAEELKQHDKPGDLWISIQGKVYNVTDWAKVHPGGDIPLLNLAGQDVTDAFIAYHP 64

Query: 416  GSAWQHLDKFFTGYHLIDYKVSDVSRDYRKLAAEFAKAGMFEKKGHIVIYSICFVALLLS 595
            G+AWQ+LDKFFTGY+L D+KVS+VS+D+RKLA EF+K G+FEKKGH ++Y+   +  L  
Sbjct: 65   GTAWQYLDKFFTGYYLKDFKVSEVSKDFRKLANEFSKLGLFEKKGHGILYTCSIIGFLFM 124

Query: 596  ACIYGVLSSDSFAVHMLSGVLLGFTWIQVAYMGHDAGHYQAMSSRGWNKVAGILIGNCIT 775
             CIYGVL  DSF VH+    LLGF WIQ +Y+GHD+GHYQ MSS G+NK+A +LIGNCIT
Sbjct: 125  VCIYGVLCVDSFWVHLGCAALLGFLWIQSSYLGHDSGHYQIMSSPGFNKLAQMLIGNCIT 184

Query: 776  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSDSFFQSLTSKFYNRKLTFDPVAKF 955
            GISIAWWKWTHNAHHIACNSLDYDPDLQH+P+ AVS S F S+ S FY R L FD VA+F
Sbjct: 185  GISIAWWKWTHNAHHIACNSLDYDPDLQHIPVFAVSSSLFDSINSVFYGRILKFDSVARF 244

Query: 956  FVSYQHLSYYPVMCVARVNLYLQTLLLLCSKRKIPDRAFNILGTIIFWTWFPLLVSCLPN 1135
            FVSYQH +YYPVMCVAR+NL+LQTLLLL S R IPDRA NI+G  +FWTW+PLLVSCLPN
Sbjct: 245  FVSYQHWTYYPVMCVARINLFLQTLLLLFSGRNIPDRALNIMGICVFWTWYPLLVSCLPN 304

Query: 1136 WTERVLFVLISFCVCAIQHVQFTLNHFAADVYVGPPTGNNWFEKQSGGT 1282
            W ERV+FV++S  V +IQH+QFTLNHF+A+VYVGPP GN+WFEKQ+ GT
Sbjct: 305  WGERVMFVIVSMAVTSIQHIQFTLNHFSANVYVGPPCGNDWFEKQTSGT 353



 Score = 94.4 bits (233), Expect(2) = e-177
 Identities = 46/68 (67%), Positives = 54/68 (79%), Gaps = 8/68 (11%)
 Frame = +1

Query: 1381 RLPRTHLRSISPIVQDLCKKHNLTYRSLSFYEANVATLKTLKTAAYQAR--------GLL 1536
            RLPR HLRSISP+V+DLCKKHNL YRSLSF+EANV+T++TL+TAA QAR         L+
Sbjct: 380  RLPRCHLRSISPVVKDLCKKHNLPYRSLSFWEANVSTIRTLRTAALQARDLTNPVPKNLV 439

Query: 1537 WEAVNTHG 1560
            WEA N HG
Sbjct: 440  WEAANIHG 447


>ref|XP_002302613.1| predicted protein [Populus trichocarpa] gi|222844339|gb|EEE81886.1|
            predicted protein [Populus trichocarpa]
          Length = 447

 Score =  557 bits (1436), Expect(2) = e-175
 Identities = 253/349 (72%), Positives = 295/349 (84%)
 Frame = +2

Query: 236  KKYITSEELGKHNKREDLWISIQGKVYNVTEWAKEHPGGDIPLLNMAGQDVTDAFIAFHP 415
            KKYIT EEL +HNK  DLWISIQGKVYNV++WA EHPGGD+ LLN+AGQDVTDAFIA+HP
Sbjct: 5    KKYITGEELKQHNKEGDLWISIQGKVYNVSDWAYEHPGGDVALLNLAGQDVTDAFIAYHP 64

Query: 416  GSAWQHLDKFFTGYHLIDYKVSDVSRDYRKLAAEFAKAGMFEKKGHIVIYSICFVALLLS 595
            G+AWQ+LDKFFTGY+L D+KVS+ S+DYR+LA+EFAK G+FEKKGHI +Y++  +AL+  
Sbjct: 65   GTAWQYLDKFFTGYYLKDFKVSETSKDYRRLASEFAKLGLFEKKGHITMYALASIALMFC 124

Query: 596  ACIYGVLSSDSFAVHMLSGVLLGFTWIQVAYMGHDAGHYQAMSSRGWNKVAGILIGNCIT 775
              +YGVL   S   H+ S ++LGF WIQ AY+GHD+GHYQ M SRG+NK A  + GN +T
Sbjct: 125  VVLYGVLCCQSVWAHLGSALVLGFLWIQSAYIGHDSGHYQVMKSRGYNKFAQFVAGNSLT 184

Query: 776  GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSDSFFQSLTSKFYNRKLTFDPVAKF 955
            GISIAWWKWTHNAHH+ACNSLDYDPDLQH+P+ AV+ +FF S+ S FY R L FDPVA+F
Sbjct: 185  GISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVNSTFFNSIKSCFYGRYLDFDPVARF 244

Query: 956  FVSYQHLSYYPVMCVARVNLYLQTLLLLCSKRKIPDRAFNILGTIIFWTWFPLLVSCLPN 1135
            FVSYQH ++YPVMCVARVNLYLQT LLL SKRK PDRA NILG +IFWTWFPLLVSCLPN
Sbjct: 245  FVSYQHWTFYPVMCVARVNLYLQTFLLLFSKRKFPDRALNILGILIFWTWFPLLVSCLPN 304

Query: 1136 WTERVLFVLISFCVCAIQHVQFTLNHFAADVYVGPPTGNNWFEKQSGGT 1282
            W ERV+FVL SF V AIQHVQF LNHFAADVY GPP GN+WFEKQ+GGT
Sbjct: 305  WPERVMFVLASFAVTAIQHVQFCLNHFAADVYTGPPKGNDWFEKQTGGT 353



 Score = 87.8 bits (216), Expect(2) = e-175
 Identities = 43/68 (63%), Positives = 51/68 (75%), Gaps = 8/68 (11%)
 Frame = +1

Query: 1381 RLPRTHLRSISPIVQDLCKKHNLTYRSLSFYEANVATLKTLKTAAYQAR--------GLL 1536
            R+PR  LR +SP+VQDLCKKHNL+YRSLSF+EANV T++ L+  A QAR         LL
Sbjct: 380  RMPRCQLRRVSPLVQDLCKKHNLSYRSLSFWEANVWTIRRLRNVAQQARDLSNPVPKNLL 439

Query: 1537 WEAVNTHG 1560
            WEAVNTHG
Sbjct: 440  WEAVNTHG 447