BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Angelica22_contig00009537
         (2156 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283154.1| PREDICTED: glutamyl-tRNA synthetase, chlorop...   889   0.0  
emb|CAN72214.1| hypothetical protein VITISV_039082 [Vitis vinifera]   865   0.0  
ref|XP_004144903.1| PREDICTED: glutamate--tRNA ligase, chloropla...   862   0.0  
gb|AAY18610.1| glutamyl-tRNA synthetase [Nicotiana benthamiana]       862   0.0  
sp|Q43794.1|SYE_TOBAC RecName: Full=Glutamate--tRNA ligase, chlo...   860   0.0  

>ref|XP_002283154.1| PREDICTED: glutamyl-tRNA synthetase, chloroplastic/mitochondrial
            [Vitis vinifera] gi|297744457|emb|CBI37719.3| unnamed
            protein product [Vitis vinifera]
          Length = 566

 Score =  889 bits (2298), Expect = 0.0
 Identities = 444/576 (77%), Positives = 488/576 (84%)
 Frame = +3

Query: 138  PWARFRGGFAEVAPPCLRQSSPLSPAFVHCRKNINFIGRFRASSNRCLSIVSASRSSVLQ 317
            PW + R    EVAPP LR+SS L                FR    R  S+  ++ +   +
Sbjct: 8    PWMKIRV-IPEVAPPILRRSSSL----------------FR----RSFSVSCSTEAPPKK 46

Query: 318  EENGVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTKESEEAVL 497
             E  VRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTK+SEEA+L
Sbjct: 47   LEGEVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTKQSEEALL 106

Query: 498  QDLSWLGLHWDEGPDVGGDYGPYRQSERNALYKQYAEELVNSGHAYRCFCSNEELEKMKE 677
            QDLSWLGLHWDEGP VGGDYGPYRQSERN+LYKQYAE+L+ SGH Y+CFCSNEELEKMKE
Sbjct: 107  QDLSWLGLHWDEGPGVGGDYGPYRQSERNSLYKQYAEKLLESGHVYQCFCSNEELEKMKE 166

Query: 678  IAKSKQMPPVYTGKWASATDEEVQEEMSKGTPFTYRFRVPKEGSLKINDLIRGEVIWNFD 857
            IAK KQ+PPVYTGKWASAT EEVQEE+ +GTP+TYRFRVPKEGSLKI+DLIRGEV WN D
Sbjct: 167  IAKLKQLPPVYTGKWASATAEEVQEELERGTPYTYRFRVPKEGSLKIDDLIRGEVRWNLD 226

Query: 858  TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGFRMPYFA 1037
            TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQAL+YKALGF MP FA
Sbjct: 227  TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALLYKALGFAMPSFA 286

Query: 1038 HVSLILAPDKSKLSKRHGATSVGQFKEMGYLPQAMINYLALLGWGDGTENEFFTLEQLVE 1217
            HVSLILAPD+SKLSKRHGATSVGQFKEMG+LPQAM+NYLALLGWGDGTENEFFTL+ LVE
Sbjct: 287  HVSLILAPDRSKLSKRHGATSVGQFKEMGFLPQAMVNYLALLGWGDGTENEFFTLKHLVE 346

Query: 1218 KFSIERVNKSGAVFDSTKFRWMNGQHLRLLPPEELTKLLGHHWKRTGILKESEGIFVEEA 1397
            KFSI+RVNK GA+FDSTK RWMNGQHLR LP EELTKL+G  WK TGIL +S+G F+EEA
Sbjct: 347  KFSIDRVNKGGAIFDSTKLRWMNGQHLRSLPSEELTKLIGERWKNTGILTQSDGPFIEEA 406

Query: 1398 VQLLKDAVDLITDSEKALSNFLSYPLYETLSSSEAKPILEDGISEFADSLLAAYDSGELF 1577
            V LLKD +DLITDS+KALSN LSYPLY TL+S E KP+LED +SE + SLLAAYDSGEL 
Sbjct: 407  VALLKDGIDLITDSDKALSNLLSYPLYATLTSPEGKPVLEDKLSEVSSSLLAAYDSGELL 466

Query: 1578 DALDQGKPGWQKWVKSFGKSAKRKGKSLFMPLRVLLTGKLHGPDIGLSVVLLRTVGTGDI 1757
             AL++G  GWQKWVKSFGKS KRKGKSLFMPLRVLLTGKLHGPD+G SV+LL   GT  +
Sbjct: 467  QALEEGPAGWQKWVKSFGKSLKRKGKSLFMPLRVLLTGKLHGPDMGASVLLLHRAGTCGV 526

Query: 1758 VGSQVGFVTLEERLKLLREVNWESFNNNQTVLESVA 1865
            V  QVGFVTLE+R K+LREV WE+ N +Q  LES A
Sbjct: 527  VAPQVGFVTLEDRFKMLREVEWEALNKDQPPLESAA 562


>emb|CAN72214.1| hypothetical protein VITISV_039082 [Vitis vinifera]
          Length = 555

 Score =  865 bits (2236), Expect = 0.0
 Identities = 436/576 (75%), Positives = 480/576 (83%)
 Frame = +3

Query: 138  PWARFRGGFAEVAPPCLRQSSPLSPAFVHCRKNINFIGRFRASSNRCLSIVSASRSSVLQ 317
            PW + R    EVAPP LR+SS L                FR    R  S+  +  +   +
Sbjct: 8    PWMKIRV-IPEVAPPILRRSSSL----------------FR----RSFSVSCSXEAPPKK 46

Query: 318  EENGVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTKESEEAVL 497
             E  VRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTK+SEEA+L
Sbjct: 47   LEGEVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTKQSEEALL 106

Query: 498  QDLSWLGLHWDEGPDVGGDYGPYRQSERNALYKQYAEELVNSGHAYRCFCSNEELEKMKE 677
            QDLSWLGLHWDEGP VGGDYGPYRQSERN+LYKQ+AE+L+ SGH Y+CFCSNEELEKMKE
Sbjct: 107  QDLSWLGLHWDEGPGVGGDYGPYRQSERNSLYKQHAEKLLESGHVYQCFCSNEELEKMKE 166

Query: 678  IAKSKQMPPVYTGKWASATDEEVQEEMSKGTPFTYRFRVPKEGSLKINDLIRGEVIWNFD 857
            IAK  Q+PPVYTGKWASAT EEVQEE+ +GTP+TYRFRVPKEGSLKI+DLIRGEV WN D
Sbjct: 167  IAKLXQLPPVYTGKWASATAEEVQEELERGTPYTYRFRVPKEGSLKIDDLIRGEVRWNLD 226

Query: 858  TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGFRMPYFA 1037
            TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQAL+YKALGF MP FA
Sbjct: 227  TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALLYKALGFAMPSFA 286

Query: 1038 HVSLILAPDKSKLSKRHGATSVGQFKEMGYLPQAMINYLALLGWGDGTENEFFTLEQLVE 1217
            HVSLILAPD+SKLSKRHGATSVGQFKEMG+LPQAM+NYLALLGWGDGTENEFFTL+ LVE
Sbjct: 287  HVSLILAPDRSKLSKRHGATSVGQFKEMGFLPQAMVNYLALLGWGDGTENEFFTLKHLVE 346

Query: 1218 KFSIERVNKSGAVFDSTKFRWMNGQHLRLLPPEELTKLLGHHWKRTGILKESEGIFVEEA 1397
            KFSI+RVNK GA+FDSTK RWMNGQHLR LP EELTKL+G  WK TGIL +S+G FVEEA
Sbjct: 347  KFSIDRVNKGGAIFDSTKLRWMNGQHLRSLPSEELTKLIGERWKNTGILTQSDGPFVEEA 406

Query: 1398 VQLLKDAVDLITDSEKALSNFLSYPLYETLSSSEAKPILEDGISEFADSLLAAYDSGELF 1577
            V  LKD +DLITDS+KALSN LSYPLY TL+S E KP+LED +SE + SLLA YDSGEL 
Sbjct: 407  VAXLKDGIDLITDSDKALSNLLSYPLYATLTSPEGKPVLEDKLSEVSSSLLATYDSGELL 466

Query: 1578 DALDQGKPGWQKWVKSFGKSAKRKGKSLFMPLRVLLTGKLHGPDIGLSVVLLRTVGTGDI 1757
             AL++G  GWQKWVKSFGKS KRKGKSLFMPLRVLLTGKLHGPD+G S++LL        
Sbjct: 467  QALEEGPAGWQKWVKSFGKSLKRKGKSLFMPLRVLLTGKLHGPDMGASLLLL-------- 518

Query: 1758 VGSQVGFVTLEERLKLLREVNWESFNNNQTVLESVA 1865
               QVGFVTLE+R K+LREV WE+ N ++  LES A
Sbjct: 519  ---QVGFVTLEDRFKMLREVZWEALNKDRPPLESAA 551


>ref|XP_004144903.1| PREDICTED: glutamate--tRNA ligase, chloroplastic/mitochondrial-like
            [Cucumis sativus] gi|449473003|ref|XP_004153756.1|
            PREDICTED: glutamate--tRNA ligase,
            chloroplastic/mitochondrial-like [Cucumis sativus]
            gi|449517148|ref|XP_004165608.1| PREDICTED:
            glutamate--tRNA ligase, chloroplastic/mitochondrial-like
            [Cucumis sativus]
          Length = 573

 Score =  862 bits (2228), Expect = 0.0
 Identities = 426/576 (73%), Positives = 489/576 (84%)
 Frame = +3

Query: 138  PWARFRGGFAEVAPPCLRQSSPLSPAFVHCRKNINFIGRFRASSNRCLSIVSASRSSVLQ 317
            PW R +  F  + PP L +S+            +  I +FR S +     VSAS   V  
Sbjct: 8    PWVRIKA-FPILNPPILHRSNFYF--------GVRCIPKFRRSFS-----VSASVDKV-- 51

Query: 318  EENGVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTKESEEAVL 497
            +   VRVRFAPSPTGNLHVGGARTALFNYLFAR+ GGKFVLRIEDTDLERST++SEEAVL
Sbjct: 52   DGGDVRVRFAPSPTGNLHVGGARTALFNYLFARANGGKFVLRIEDTDLERSTRQSEEAVL 111

Query: 498  QDLSWLGLHWDEGPDVGGDYGPYRQSERNALYKQYAEELVNSGHAYRCFCSNEELEKMKE 677
            +DLSWLGL WDEGP+VGG+YGPYRQSERNALYKQYAE+L+ SG  YRCFCSNEELEKMKE
Sbjct: 112  RDLSWLGLDWDEGPNVGGEYGPYRQSERNALYKQYAEKLLESGQVYRCFCSNEELEKMKE 171

Query: 678  IAKSKQMPPVYTGKWASATDEEVQEEMSKGTPFTYRFRVPKEGSLKINDLIRGEVIWNFD 857
            +AK KQ+PPVY GKWASAT+EEV+EE+ KGTP+T+RFRVP+EGSLKINDLIRGEV WN D
Sbjct: 172  VAKLKQLPPVYMGKWASATNEEVREELEKGTPYTFRFRVPQEGSLKINDLIRGEVSWNLD 231

Query: 858  TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGFRMPYFA 1037
            TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGF MPYFA
Sbjct: 232  TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGFPMPYFA 291

Query: 1038 HVSLILAPDKSKLSKRHGATSVGQFKEMGYLPQAMINYLALLGWGDGTENEFFTLEQLVE 1217
            HVSLILAPD+SKLSKRHGATSVGQF+EMGYLP+AM+NYLALLGWGDGTENEFFTLEQLVE
Sbjct: 292  HVSLILAPDRSKLSKRHGATSVGQFREMGYLPEAMVNYLALLGWGDGTENEFFTLEQLVE 351

Query: 1218 KFSIERVNKSGAVFDSTKFRWMNGQHLRLLPPEELTKLLGHHWKRTGILKESEGIFVEEA 1397
            KFSI RVNKSGAVFDSTK RWMNGQHLR LP EELTKL+G  WK TGIL ESEG F+EE+
Sbjct: 352  KFSISRVNKSGAVFDSTKLRWMNGQHLRALPSEELTKLIGERWKSTGILTESEGPFIEES 411

Query: 1398 VQLLKDAVDLITDSEKALSNFLSYPLYETLSSSEAKPILEDGISEFADSLLAAYDSGELF 1577
            VQLLKDA+DL+TD++KALSN LSYPL+ TL+SSEAKP++ED +SEF+ SL+ AYDSGE+ 
Sbjct: 412  VQLLKDAIDLVTDADKALSNLLSYPLHTTLTSSEAKPLVEDKLSEFSASLITAYDSGEIL 471

Query: 1578 DALDQGKPGWQKWVKSFGKSAKRKGKSLFMPLRVLLTGKLHGPDIGLSVVLLRTVGTGDI 1757
            +AL++G  GWQKWVKSFGKS KRKGKSLFMPLR+LLTGK+HGPD+G S+VLL    +  +
Sbjct: 472  NALEEGPSGWQKWVKSFGKSLKRKGKSLFMPLRLLLTGKIHGPDMGASIVLLHKARSSGV 531

Query: 1758 VGSQVGFVTLEERLKLLREVNWESFNNNQTVLESVA 1865
            V  Q GFV L +R ++LR+++W++   +  ++ES A
Sbjct: 532  VAPQAGFVPLNDRFEILRQIDWDAVTKDSPLVESPA 567


>gb|AAY18610.1| glutamyl-tRNA synthetase [Nicotiana benthamiana]
          Length = 569

 Score =  862 bits (2227), Expect = 0.0
 Identities = 433/585 (74%), Positives = 488/585 (83%)
 Frame = +3

Query: 120  TLLDKIPWARFRGGFAEVAPPCLRQSSPLSPAFVHCRKNINFIGRFRASSNRCLSIVSAS 299
            T L   PW R     A + P    + S L     +CR + ++  +   S  R  S+ +++
Sbjct: 2    TTLAAAPWFR-----ARLIPELKNRQSLL-----YCRGDHSY-WQSLCSRRRSFSVYASA 50

Query: 300  RSSVLQEENGVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTKE 479
             +        VRVRFAPSPTGNLHVGGARTALFNYL+AR+KGGKF+LRIEDTDLERSTKE
Sbjct: 51   GNG-----GDVRVRFAPSPTGNLHVGGARTALFNYLYARAKGGKFILRIEDTDLERSTKE 105

Query: 480  SEEAVLQDLSWLGLHWDEGPDVGGDYGPYRQSERNALYKQYAEELVNSGHAYRCFCSNEE 659
            SEEAVL+DLSWLGL WDEGP +GG YGPYRQSERNALYKQ+AE+L+ SGH YRCFCSNEE
Sbjct: 106  SEEAVLRDLSWLGLAWDEGPGIGGGYGPYRQSERNALYKQFAEKLLQSGHVYRCFCSNEE 165

Query: 660  LEKMKEIAKSKQMPPVYTGKWASATDEEVQEEMSKGTPFTYRFRVPKEGSLKINDLIRGE 839
            LEKMKEIAK KQ+PPVYTG+WASAT+EEV EE++KGTPFTYRFRVPKEGSLKI+DLIRGE
Sbjct: 166  LEKMKEIAKLKQLPPVYTGRWASATEEEVVEELAKGTPFTYRFRVPKEGSLKIDDLIRGE 225

Query: 840  VIWNFDTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGF 1019
            V WN DTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGF
Sbjct: 226  VSWNLDTLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGF 285

Query: 1020 RMPYFAHVSLILAPDKSKLSKRHGATSVGQFKEMGYLPQAMINYLALLGWGDGTENEFFT 1199
             MP+FAHVSLILAPD+SKLSKRHGATSVGQF++MGYLPQAM+NYLALLGWGDGTENEFFT
Sbjct: 286  PMPHFAHVSLILAPDRSKLSKRHGATSVGQFRDMGYLPQAMVNYLALLGWGDGTENEFFT 345

Query: 1200 LEQLVEKFSIERVNKSGAVFDSTKFRWMNGQHLRLLPPEELTKLLGHHWKRTGILKESEG 1379
            LEQLVEKF+IERVNKSGA+FDSTK RWMNGQHLR LP EEL +++G  WK  GI  ES+G
Sbjct: 346  LEQLVEKFTIERVNKSGAIFDSTKLRWMNGQHLRSLPSEELNRIIGERWKDAGITAESQG 405

Query: 1380 IFVEEAVQLLKDAVDLITDSEKALSNFLSYPLYETLSSSEAKPILEDGISEFADSLLAAY 1559
            IF+++AV LLKD +DLITDSEKALS+ LSYPLYETL+S+E KPILEDG+SE A SLLAAY
Sbjct: 406  IFIQDAVLLLKDGIDLITDSEKALSSLLSYPLYETLASAEGKPILEDGVSEVAKSLLAAY 465

Query: 1560 DSGELFDALDQGKPGWQKWVKSFGKSAKRKGKSLFMPLRVLLTGKLHGPDIGLSVVLLRT 1739
            DSGE   AL +G+PGWQKWVK+FGK  KRKGKSLFMPLRVLLTGKLHGPDIG + VLL  
Sbjct: 466  DSGEFSGALAEGQPGWQKWVKNFGKLLKRKGKSLFMPLRVLLTGKLHGPDIGATTVLLYK 525

Query: 1740 VGTGDIVGSQVGFVTLEERLKLLREVNWESFNNNQTVLESVAASH 1874
             GT   V  Q GFVT +ER K+LREV WESF+ +   L + A +H
Sbjct: 526  AGTSGSVVPQAGFVTFDERFKILREVQWESFSTD-VPLSAGAVTH 569


>sp|Q43794.1|SYE_TOBAC RecName: Full=Glutamate--tRNA ligase, chloroplastic/mitochondrial;
            AltName: Full=Glutamyl-tRNA synthetase; Short=GluRS;
            Flags: Precursor gi|603867|emb|CAA58506.1|
            glutamate--tRNA ligase [Nicotiana tabacum]
          Length = 569

 Score =  860 bits (2223), Expect = 0.0
 Identities = 428/568 (75%), Positives = 481/568 (84%)
 Frame = +3

Query: 138  PWARFRGGFAEVAPPCLRQSSPLSPAFVHCRKNINFIGRFRASSNRCLSIVSASRSSVLQ 317
            PW R R        P L+ S  L    ++CR N ++  +   S  R  S+ +++      
Sbjct: 8    PWFRVR------LIPELKNSQSL----LYCRGNHSY-RQSLCSRRRSFSVYASAGDG--- 53

Query: 318  EENGVRVRFAPSPTGNLHVGGARTALFNYLFARSKGGKFVLRIEDTDLERSTKESEEAVL 497
                VRVRFAPSPTGNLHVGGARTALFNYL+AR+KGGKF+LRIEDTDLERSTKESEEAVL
Sbjct: 54   --GDVRVRFAPSPTGNLHVGGARTALFNYLYARAKGGKFILRIEDTDLERSTKESEEAVL 111

Query: 498  QDLSWLGLHWDEGPDVGGDYGPYRQSERNALYKQYAEELVNSGHAYRCFCSNEELEKMKE 677
            +DLSWLG  WDEGP +GG+YGPYRQSERNALYKQ+AE+L+ SGH YRCFCSNEELEKMKE
Sbjct: 112  RDLSWLGPAWDEGPGIGGEYGPYRQSERNALYKQFAEKLLQSGHVYRCFCSNEELEKMKE 171

Query: 678  IAKSKQMPPVYTGKWASATDEEVQEEMSKGTPFTYRFRVPKEGSLKINDLIRGEVIWNFD 857
            IAK KQ+PPVYTG+WASAT+EEV EE++KGTP+TYRFRVPKEGSLKI+DLIRGEV WN D
Sbjct: 172  IAKLKQLPPVYTGRWASATEEEVVEELAKGTPYTYRFRVPKEGSLKIDDLIRGEVSWNLD 231

Query: 858  TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGFRMPYFA 1037
            TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGF MP+FA
Sbjct: 232  TLGDFVIMRSNGQPVYNFCVTVDDATMAISHVIRAEEHLPNTLRQALIYKALGFPMPHFA 291

Query: 1038 HVSLILAPDKSKLSKRHGATSVGQFKEMGYLPQAMINYLALLGWGDGTENEFFTLEQLVE 1217
            HVSLILAPD+SKLSKRHGATSVGQF++MGYLPQAM+NYLALLGWGDGTENEFFTLEQLVE
Sbjct: 292  HVSLILAPDRSKLSKRHGATSVGQFRDMGYLPQAMVNYLALLGWGDGTENEFFTLEQLVE 351

Query: 1218 KFSIERVNKSGAVFDSTKFRWMNGQHLRLLPPEELTKLLGHHWKRTGILKESEGIFVEEA 1397
            KF+IERVNKSGA+FDSTK RWMNGQHLR LP EEL +++G  WK  GI  ES+GIF+++A
Sbjct: 352  KFTIERVNKSGAIFDSTKLRWMNGQHLRSLPSEELNRIIGERWKDAGIATESQGIFIQDA 411

Query: 1398 VQLLKDAVDLITDSEKALSNFLSYPLYETLSSSEAKPILEDGISEFADSLLAAYDSGELF 1577
            V LLKD +DLITDSEKALS+ LSYPLYETL+S+E KPILEDG+SE A SLLAAYDSGEL 
Sbjct: 412  VLLLKDGIDLITDSEKALSSLLSYPLYETLASAEGKPILEDGVSEVAKSLLAAYDSGELS 471

Query: 1578 DALDQGKPGWQKWVKSFGKSAKRKGKSLFMPLRVLLTGKLHGPDIGLSVVLLRTVGTGDI 1757
             AL +G+PGWQKW K+FGK  KRKGKSLFMPLRVLLTGKLHGPDIG + VLL   GT   
Sbjct: 472  GALAEGQPGWQKWAKNFGKLLKRKGKSLFMPLRVLLTGKLHGPDIGATTVLLYKAGTSGS 531

Query: 1758 VGSQVGFVTLEERLKLLREVNWESFNNN 1841
            V  Q GFVT +ER K+LREV WESF+ +
Sbjct: 532  VVPQAGFVTFDERFKILREVQWESFSTD 559