BLASTX nr result
ID: Anemarrhena21_contig00058397
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Anemarrhena21_contig00058397
(333 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_008095924.1| quinone oxidoreductase [Colletotrichum grami... 143 4e-32
ref|XP_001794673.1| hypothetical protein SNOG_04253 [Phaeosphaer... 142 9e-32
sp|P42058.1|ALTA7_ALTAL RecName: Full=Minor allergen Alt a 7; Al... 140 3e-31
ref|XP_007716655.1| benzoquinone reductase [Bipolaris zeicola 26... 140 4e-31
ref|XP_657901.1| hypothetical protein AN0297.2 [Aspergillus nidu... 139 6e-31
ref|XP_009217644.1| minor allergen Alt a 7 [Gaeumannomyces grami... 139 6e-31
gb|KLU87818.1| minor allergen Alt a 7 [Magnaporthiopsis poae ATC... 139 1e-30
ref|XP_003049699.1| predicted protein [Nectria haematococca mpVI... 139 1e-30
ref|XP_001938161.1| minor allergen Alt a 7 [Pyrenophora tritici-... 139 1e-30
ref|XP_003298406.1| hypothetical protein PTT_09127 [Pyrenophora ... 138 2e-30
ref|XP_007682524.1| benzoquinone reductase [Bipolaris oryzae ATC... 137 2e-30
gb|EMD94134.1| hypothetical protein COCHEDRAFT_1130430 [Bipolari... 137 2e-30
ref|XP_007701342.1| hypothetical protein COCSADRAFT_145223 [Bipo... 137 2e-30
gb|KDN60371.1| putative quinone oxidoreductase [Colletotrichum s... 137 4e-30
ref|XP_007597494.1| minor allergen Alt a 7 [Colletotrichum fiori... 137 4e-30
gb|KKY18404.1| putative nadh-quinone oxidoreductase [Phaeomoniel... 136 6e-30
ref|XP_007916415.1| putative minor allergen alt a 7 protein [Tog... 135 8e-30
emb|CEF73529.1| unnamed protein product [Fusarium graminearum] 135 1e-29
ref|XP_009259947.1| hypothetical protein FPSE_08554 [Fusarium ps... 135 1e-29
ref|XP_001220568.1| hypothetical protein CHGG_01347 [Chaetomium ... 134 2e-29
>ref|XP_008095924.1| quinone oxidoreductase [Colletotrichum graminicola M1.001]
gi|310796443|gb|EFQ31904.1| quinone oxidoreductase
[Colletotrichum graminicola M1.001]
Length = 204
Score = 143 bits (361), Expect = 4e-32
Identities = 71/87 (81%), Positives = 77/87 (88%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSD- 84
MAPKIAIVYYSMYGH++ LA+AE KGI++AGGSADLFQIAETLPEEVL KMHAPPK SD
Sbjct: 1 MAPKIAIVYYSMYGHIRQLAEAEKKGIEKAGGSADLFQIAETLPEEVLAKMHAPPKPSDI 60
Query: 83 TVLSDPAQLEPYDAVLFGIPTRYGNFP 3
VL+DPA LE YDA L GIPTRYGNFP
Sbjct: 61 PVLNDPATLEAYDAFLIGIPTRYGNFP 87
>ref|XP_001794673.1| hypothetical protein SNOG_04253 [Phaeosphaeria nodorum SN15]
gi|111066893|gb|EAT88013.1| hypothetical protein
SNOG_04253 [Phaeosphaeria nodorum SN15]
Length = 204
Score = 142 bits (358), Expect = 9e-32
Identities = 69/87 (79%), Positives = 77/87 (88%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH+K +ADAELKGI+EAGG A LFQI ETL +EVLGKMHAPPK +D
Sbjct: 1 MAPKIAIVYYSMYGHIKQMADAELKGIKEAGGDATLFQIPETLSDEVLGKMHAPPKPTDV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
L+DPA+LE +DAVLFGIPTRYGNFP
Sbjct: 61 PTLNDPAELEAFDAVLFGIPTRYGNFP 87
>sp|P42058.1|ALTA7_ALTAL RecName: Full=Minor allergen Alt a 7; AltName: Full=Allergen Alt a
VII; AltName: Allergen=Alt a 7 [Alternaria alternata]
gi|467619|emb|CAA55069.1| minor allergen [Alternaria
alternata]
Length = 204
Score = 140 bits (353), Expect = 3e-31
Identities = 69/87 (79%), Positives = 76/87 (87%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPK-SSD 84
MAPKIAIVYYSMYGH+K +ADAELKGIQEAGG A LFQ+AETLP+EVL KM+APPK SS
Sbjct: 1 MAPKIAIVYYSMYGHIKKMADAELKGIQEAGGDAKLFQVAETLPQEVLDKMYAPPKDSSV 60
Query: 83 TVLSDPAQLEPYDAVLFGIPTRYGNFP 3
VL DPA LE +D +LFGIPTRYGNFP
Sbjct: 61 PVLEDPAVLEEFDGILFGIPTRYGNFP 87
>ref|XP_007716655.1| benzoquinone reductase [Bipolaris zeicola 26-R-13]
gi|576914697|gb|EUC29036.1| benzoquinone reductase
[Bipolaris zeicola 26-R-13] gi|578484009|gb|EUN21550.1|
benzoquinone reductase [Bipolaris victoriae FI3]
Length = 204
Score = 140 bits (352), Expect = 4e-31
Identities = 69/87 (79%), Positives = 76/87 (87%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH+K LADAELKGIQEAGG A LFQ+AETLPEEVL KM+A PK++D
Sbjct: 1 MAPKIAIVYYSMYGHIKKLADAELKGIQEAGGDAKLFQVAETLPEEVLQKMYAGPKAADV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
VL DPA LE +D +LFGIPTRYGNFP
Sbjct: 61 PVLEDPAVLEEFDGILFGIPTRYGNFP 87
>ref|XP_657901.1| hypothetical protein AN0297.2 [Aspergillus nidulans FGSC A4]
gi|40746547|gb|EAA65703.1| hypothetical protein AN0297.2
[Aspergillus nidulans FGSC A4]
gi|259489476|tpe|CBF89779.1| TPA: NADH-quinone
oxidoreductase Pst2, putative (AFU_orthologue;
AFUA_1G02820) [Aspergillus nidulans FGSC A4]
Length = 204
Score = 139 bits (351), Expect = 6e-31
Identities = 69/86 (80%), Positives = 73/86 (84%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIV+YSMYGHV+ LA AELKGIQ AGG+ADL+QI ETLPEEVL KMHAPPKSS
Sbjct: 1 MAPKIAIVFYSMYGHVEKLAQAELKGIQAAGGNADLYQIQETLPEEVLAKMHAPPKSSVP 60
Query: 80 VLSDPAQLEPYDAVLFGIPTRYGNFP 3
L P QL YDAVLFGIPTRYGNFP
Sbjct: 61 TLEKPEQLLEYDAVLFGIPTRYGNFP 86
>ref|XP_009217644.1| minor allergen Alt a 7 [Gaeumannomyces graminis var. tritici
R3-111a-1] gi|402086737|gb|EJT81635.1| minor allergen
Alt a 7 [Gaeumannomyces graminis var. tritici R3-111a-1]
Length = 203
Score = 139 bits (351), Expect = 6e-31
Identities = 68/86 (79%), Positives = 74/86 (86%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIV+YSMYGH+K LADAE GI++AGG+ADLFQI ETL E+VLGKMHAP KS T
Sbjct: 1 MAPKIAIVFYSMYGHIKQLADAEKAGIEKAGGTADLFQIEETLSEQVLGKMHAPAKSDVT 60
Query: 80 VLSDPAQLEPYDAVLFGIPTRYGNFP 3
VLSDPA LE YD LFGIPTRYGNFP
Sbjct: 61 VLSDPATLEGYDGFLFGIPTRYGNFP 86
>gb|KLU87818.1| minor allergen Alt a 7 [Magnaporthiopsis poae ATCC 64411]
Length = 204
Score = 139 bits (349), Expect = 1e-30
Identities = 66/86 (76%), Positives = 72/86 (83%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIV+YSMYGH+K LA+AE G++ AGG+ADLFQI ETLPEEVLGKMHAPPKS
Sbjct: 1 MAPKIAIVFYSMYGHIKQLAEAEKAGVERAGGTADLFQIEETLPEEVLGKMHAPPKSDVA 60
Query: 80 VLSDPAQLEPYDAVLFGIPTRYGNFP 3
VL DPA L YD LFGIPTRYGNFP
Sbjct: 61 VLKDPATLAGYDGFLFGIPTRYGNFP 86
>ref|XP_003049699.1| predicted protein [Nectria haematococca mpVI 77-13-4]
gi|256730635|gb|EEU43986.1| predicted protein [Nectria
haematococca mpVI 77-13-4]
Length = 204
Score = 139 bits (349), Expect = 1e-30
Identities = 67/87 (77%), Positives = 76/87 (87%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH+KTLA+AE GI++AGGSADL+QI ETLPE+VL KMHAPPK +D
Sbjct: 1 MAPKIAIVYYSMYGHIKTLAEAEKAGIEKAGGSADLYQIPETLPEDVLAKMHAPPKPADV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
VL DP++LE YDA L GIPTRYGNFP
Sbjct: 61 PVLEDPSKLEEYDAFLLGIPTRYGNFP 87
>ref|XP_001938161.1| minor allergen Alt a 7 [Pyrenophora tritici-repentis Pt-1C-BFP]
gi|187985260|gb|EDU50748.1| minor allergen Alt a 7
[Pyrenophora tritici-repentis Pt-1C-BFP]
Length = 204
Score = 139 bits (349), Expect = 1e-30
Identities = 67/87 (77%), Positives = 75/87 (86%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH+K LADAELKGIQEAGG A LFQ+ ETLPEEVL KMHAP K++D
Sbjct: 1 MAPKIAIVYYSMYGHIKQLADAELKGIQEAGGEAKLFQVKETLPEEVLAKMHAPAKAADV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
V+ DP+ LE +D +LFGIPTRYGNFP
Sbjct: 61 PVVEDPSILEEFDGILFGIPTRYGNFP 87
>ref|XP_003298406.1| hypothetical protein PTT_09127 [Pyrenophora teres f. teres 0-1]
gi|311328379|gb|EFQ93483.1| hypothetical protein
PTT_09127 [Pyrenophora teres f. teres 0-1]
Length = 258
Score = 138 bits (347), Expect = 2e-30
Identities = 67/87 (77%), Positives = 75/87 (86%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH+K LADAELKGIQEAGG A LFQ+ ETLPEEVL KMHAP K++D
Sbjct: 1 MAPKIAIVYYSMYGHIKQLADAELKGIQEAGGDAKLFQVKETLPEEVLTKMHAPAKAADV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
V+ DP+ LE +D +LFGIPTRYGNFP
Sbjct: 61 PVVEDPSILEEFDGILFGIPTRYGNFP 87
>ref|XP_007682524.1| benzoquinone reductase [Bipolaris oryzae ATCC 44560]
gi|576937477|gb|EUC50965.1| benzoquinone reductase
[Bipolaris oryzae ATCC 44560]
Length = 204
Score = 137 bits (346), Expect = 2e-30
Identities = 67/87 (77%), Positives = 76/87 (87%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH+K LADAELKGIQEAGG A LFQ+AETLP+EVL KM+A PK++D
Sbjct: 1 MAPKIAIVYYSMYGHIKKLADAELKGIQEAGGDAKLFQVAETLPQEVLEKMYAGPKAADV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
VL DP+ LE +D +LFGIPTRYGNFP
Sbjct: 61 PVLDDPSVLEEFDGILFGIPTRYGNFP 87
>gb|EMD94134.1| hypothetical protein COCHEDRAFT_1130430 [Bipolaris maydis C5]
gi|477590491|gb|ENI07565.1| hypothetical protein
COCC4DRAFT_132252 [Bipolaris maydis ATCC 48331]
Length = 204
Score = 137 bits (346), Expect = 2e-30
Identities = 67/87 (77%), Positives = 76/87 (87%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH+K LADAELKGIQEAGG A LFQ+AETLP+EVL KM+A PK++D
Sbjct: 1 MAPKIAIVYYSMYGHIKKLADAELKGIQEAGGDAKLFQVAETLPQEVLEKMYAGPKAADV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
VL DP+ LE +D +LFGIPTRYGNFP
Sbjct: 61 PVLEDPSVLEEFDGILFGIPTRYGNFP 87
>ref|XP_007701342.1| hypothetical protein COCSADRAFT_145223 [Bipolaris sorokiniana
ND90Pr] gi|451849784|gb|EMD63087.1| hypothetical protein
COCSADRAFT_145223 [Bipolaris sorokiniana ND90Pr]
Length = 204
Score = 137 bits (346), Expect = 2e-30
Identities = 67/87 (77%), Positives = 76/87 (87%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH+K LADAELKGIQEAGG A LFQ+AETLP+EVL KM+A PK++D
Sbjct: 1 MAPKIAIVYYSMYGHIKKLADAELKGIQEAGGDAKLFQVAETLPQEVLEKMYAGPKAADV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
VL DP+ LE +D +LFGIPTRYGNFP
Sbjct: 61 PVLEDPSVLEEFDGILFGIPTRYGNFP 87
>gb|KDN60371.1| putative quinone oxidoreductase [Colletotrichum sublineola]
Length = 204
Score = 137 bits (344), Expect = 4e-30
Identities = 67/87 (77%), Positives = 75/87 (86%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH++ LA+AE KGI++AGG+ADLFQ+AETLPEEVL KM APPK +D
Sbjct: 1 MAPKIAIVYYSMYGHIRQLAEAEKKGIEKAGGTADLFQVAETLPEEVLAKMGAPPKPTDV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
VL DPA LE YDA L GIPTRYGNFP
Sbjct: 61 PVLDDPATLEAYDAFLIGIPTRYGNFP 87
>ref|XP_007597494.1| minor allergen Alt a 7 [Colletotrichum fioriniae PJ7]
gi|588897665|gb|EXF78905.1| minor allergen Alt a 7
[Colletotrichum fioriniae PJ7]
Length = 253
Score = 137 bits (344), Expect = 4e-30
Identities = 67/87 (77%), Positives = 76/87 (87%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH++ LA+AE KGI++AGG+ADLFQ+AETLPEEVL KM APPK++D
Sbjct: 1 MAPKIAIVYYSMYGHIRQLAEAEKKGIEKAGGTADLFQVAETLPEEVLTKMGAPPKATDV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
VL DPA LE YDA L GIPTRYGNFP
Sbjct: 61 PVLDDPATLESYDAFLLGIPTRYGNFP 87
>gb|KKY18404.1| putative nadh-quinone oxidoreductase [Phaeomoniella chlamydospora]
Length = 203
Score = 136 bits (342), Expect = 6e-30
Identities = 64/86 (74%), Positives = 76/86 (88%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPK+AIV+YS+YGH+KTLA+AE KGI+ AGGS D+FQ+AETLPE+VL KMHAPPKS
Sbjct: 1 MAPKVAIVFYSLYGHIKTLAEAEKKGIESAGGSVDIFQVAETLPEDVLTKMHAPPKSDYP 60
Query: 80 VLSDPAQLEPYDAVLFGIPTRYGNFP 3
V++ PA+L YDAVLFGIPTRYGNFP
Sbjct: 61 VIT-PAELSAYDAVLFGIPTRYGNFP 85
>ref|XP_007916415.1| putative minor allergen alt a 7 protein [Togninia minima UCRPA7]
gi|500255434|gb|EON98805.1| putative minor allergen alt
a 7 protein [Togninia minima UCRPA7]
Length = 205
Score = 135 bits (341), Expect = 8e-30
Identities = 65/87 (74%), Positives = 75/87 (86%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGHV+TLA+AE KGI++AGG+ADLFQ+AETLP+EVL KM+APPK +D
Sbjct: 1 MAPKIAIVYYSMYGHVRTLAEAEKKGIEKAGGTADLFQVAETLPQEVLDKMYAPPKPTDV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
+SDP LE YD L GIPTRYGNFP
Sbjct: 61 PTISDPTDLEGYDGFLIGIPTRYGNFP 87
>emb|CEF73529.1| unnamed protein product [Fusarium graminearum]
Length = 251
Score = 135 bits (340), Expect = 1e-29
Identities = 65/87 (74%), Positives = 75/87 (86%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH+K LA+AE GI++AGG+ADLFQ+ ETLPEEVL KMHAPPK++D
Sbjct: 1 MAPKIAIVYYSMYGHIKQLAEAEKAGIEKAGGTADLFQVPETLPEEVLAKMHAPPKATDV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
L+DP+ LE YDA L GIPTRYGNFP
Sbjct: 61 PTLNDPSILESYDAFLLGIPTRYGNFP 87
>ref|XP_009259947.1| hypothetical protein FPSE_08554 [Fusarium pseudograminearum CS3096]
gi|758189641|ref|XP_011317200.1| hypothetical protein
FGSG_01403 [Fusarium graminearum PH-1]
gi|408391949|gb|EKJ71315.1| hypothetical protein
FPSE_08554 [Fusarium pseudograminearum CS3096]
gi|558856632|gb|ESU06715.1| hypothetical protein
FGSG_01403 [Fusarium graminearum PH-1]
gi|596541905|gb|EYB22436.1| hypothetical protein
FG05_01403 [Fusarium graminearum]
Length = 204
Score = 135 bits (340), Expect = 1e-29
Identities = 65/87 (74%), Positives = 75/87 (86%), Gaps = 1/87 (1%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIVYYSMYGH+K LA+AE GI++AGG+ADLFQ+ ETLPEEVL KMHAPPK++D
Sbjct: 1 MAPKIAIVYYSMYGHIKQLAEAEKAGIEKAGGTADLFQVPETLPEEVLAKMHAPPKATDV 60
Query: 80 -VLSDPAQLEPYDAVLFGIPTRYGNFP 3
L+DP+ LE YDA L GIPTRYGNFP
Sbjct: 61 PTLNDPSILESYDAFLLGIPTRYGNFP 87
>ref|XP_001220568.1| hypothetical protein CHGG_01347 [Chaetomium globosum CBS 148.51]
gi|88185644|gb|EAQ93112.1| hypothetical protein
CHGG_01347 [Chaetomium globosum CBS 148.51]
Length = 334
Score = 134 bits (338), Expect = 2e-29
Identities = 64/86 (74%), Positives = 74/86 (86%)
Frame = -2
Query: 260 MAPKIAIVYYSMYGHVKTLADAELKGIQEAGGSADLFQIAETLPEEVLGKMHAPPKSSDT 81
MAPKIAIV+YSMYGH++ LA+AE GI++AGG+ADLFQI ETLP+EVL KMHAPPKS
Sbjct: 1 MAPKIAIVFYSMYGHIQQLAEAEKAGIEKAGGTADLFQIPETLPQEVLTKMHAPPKSDIP 60
Query: 80 VLSDPAQLEPYDAVLFGIPTRYGNFP 3
VLSDP+ L+ YDA L GIPTRYGNFP
Sbjct: 61 VLSDPSTLQQYDAFLLGIPTRYGNFP 86