BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Anemarrhena21_contig00017517
         (2192 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_008783812.1| PREDICTED: folylpolyglutamate synthase, mito...   470   e-179
ref|XP_008783811.1| PREDICTED: folylpolyglutamate synthase, mito...   466   e-177
ref|XP_009421246.1| PREDICTED: probable dihydrofolate synthetase...   480   e-173
ref|XP_010249972.1| PREDICTED: probable dihydrofolate synthetase...   414   e-152
ref|XP_010249889.1| PREDICTED: probable dihydrofolate synthetase...   407   e-150
ref|XP_007038953.1| Folylpolyglutamate synthetase family protein...   412   e-148
ref|XP_004958237.1| PREDICTED: probable dihydrofolate synthetase...   397   e-146
ref|XP_009357329.1| PREDICTED: probable dihydrofolate synthetase...   400   e-146
gb|ABA99426.2| dihydrofolate synthetase/folylpolyglutamate synth...   402   e-145
ref|XP_009368683.1| PREDICTED: probable dihydrofolate synthetase...   397   e-145
ref|XP_003577221.1| PREDICTED: probable dihydrofolate synthetase...   387   e-145
ref|XP_008376690.1| PREDICTED: probable dihydrofolate synthetase...   397   e-145
ref|XP_006664219.1| PREDICTED: putative folylpolyglutamate synth...   397   e-144
ref|XP_002442305.1| hypothetical protein SORBIDRAFT_08g017730 [S...   391   e-143
ref|XP_002267695.2| PREDICTED: probable dihydrofolate synthetase...   399   e-143
ref|XP_012090121.1| PREDICTED: probable folylpolyglutamate synth...   382   e-142
ref|XP_008234781.1| PREDICTED: dihydrofolate synthetase [Prunus ...   389   e-142
gb|KEH40416.1| tetrahydrofolylpolyglutamate synthase [Medicago t...   381   e-141
ref|XP_002513590.1| tetrahydrofolylpolyglutamate synthase, putat...   384   e-141
ref|XP_012440035.1| PREDICTED: dihydrofolate synthetase [Gossypi...   396   e-141

>ref|XP_008783812.1| PREDICTED: folylpolyglutamate synthase, mitochondrial isoform X2
            [Phoenix dactylifera]
          Length = 563

 Score =  470 bits (1210), Expect(2) = e-179
 Identities = 246/375 (65%), Positives = 297/375 (79%), Gaps = 1/375 (0%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            VFTALAF++FSQ KVDIAIIEAGLGGARDATNV+ + EL+ +VITS+GEEHLAALGGSLE
Sbjct: 176  VFTALAFLLFSQAKVDIAIIEAGLGGARDATNVICNTELSVAVITSIGEEHLAALGGSLE 235

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAKSGIIKHG P+VIGGPFE HIE IIRD+AF MNSPV+S+CDPGI+S +K  G E+
Sbjct: 236  SIAMAKSGIIKHGCPVVIGGPFEPHIEHIIRDRAFLMNSPVISSCDPGIQSVLKCSGREN 295

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            G  YQTCD+ I+VQ+DL+L IDLPN+ + MLG+HQ  NAVTATCTALCLR QG+ +SD+S
Sbjct: 296  GNSYQTCDIFIQVQKDLQLSIDLPNVKLCMLGNHQRLNAVTATCTALCLRSQGWAISDES 355

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IRAGLE+T+LLGR Q LTQ EA A+G+S   IL+DGAHTEASA+GL DVI++ +PDG LA
Sbjct: 356  IRAGLERTQLLGRSQFLTQDEASAIGLSGTSILIDGAHTEASAKGLVDVIQMMHPDGALA 415

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            LVVAMASDKDHLAFA+QLL+G RPEIVLLTEV+IAGGKSRV  ASTLKD W+R       
Sbjct: 416  LVVAMASDKDHLAFAKQLLSGPRPEIVLLTEVTIAGGKSRVASASTLKDFWIRAALDLGI 475

Query: 536  XXXXXXTIDEERGLHS-LINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNPR 360
                   ID  + L       +GS D    +L  C   S+ ++IK A Q+L+SR  + P 
Sbjct: 476  DFVDIGVIDHGKNLEGHKSESRGSSDHSPAMLVVCHIGSVSESIKAASQLLQSRIGNKPS 535

Query: 359  LIVVTGSLHIVSSML 315
            ++V TGSL++VSS+L
Sbjct: 536  VVVATGSLYVVSSVL 550



 Score =  187 bits (476), Expect(2) = e-179
 Identities = 99/156 (63%), Positives = 112/156 (71%), Gaps = 1/156 (0%)
 Frame = -3

Query: 1911 MLCFTRFISPLQHLVRRTETIALTSRLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGV 1732
            M C  R I    H+   TE   L  R  +SRL S M ED +L DFLDYME LKNYER GV
Sbjct: 1    MQCLARSIFSFCHMRIGTEVSRLAPRFLRSRLQSAMSEDPELGDFLDYMENLKNYERSGV 60

Query: 1731 PKGAGTESDDGFDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSV 1552
            P+GAGT+SDDGFD           GNPHT F+AVH+AGTKGKGSTAAFLSNIL+E GY V
Sbjct: 61   PRGAGTDSDDGFDLGRMRRLLQRLGNPHTQFKAVHIAGTKGKGSTAAFLSNILREEGYLV 120

Query: 1551 GCYTSPHLLTIRERISFGKS-EHVPAEMLMNIFHQV 1447
            GCYTSPHLLTIRERISFG++   V AE+L N+FH+V
Sbjct: 121  GCYTSPHLLTIRERISFGRNGVPVSAEVLKNVFHRV 156


>ref|XP_008783811.1| PREDICTED: folylpolyglutamate synthase, mitochondrial isoform X1
            [Phoenix dactylifera]
          Length = 564

 Score =  466 bits (1198), Expect(2) = e-177
 Identities = 246/376 (65%), Positives = 297/376 (78%), Gaps = 2/376 (0%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIE-AGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSL 1260
            VFTALAF++FSQ KVDIAIIE AGLGGARDATNV+ + EL+ +VITS+GEEHLAALGGSL
Sbjct: 176  VFTALAFLLFSQAKVDIAIIEQAGLGGARDATNVICNTELSVAVITSIGEEHLAALGGSL 235

Query: 1259 ESIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTE 1080
            ESIAMAKSGIIKHG P+VIGGPFE HIE IIRD+AF MNSPV+S+CDPGI+S +K  G E
Sbjct: 236  ESIAMAKSGIIKHGCPVVIGGPFEPHIEHIIRDRAFLMNSPVISSCDPGIQSVLKCSGRE 295

Query: 1079 DGKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDK 900
            +G  YQTCD+ I+VQ+DL+L IDLPN+ + MLG+HQ  NAVTATCTALCLR QG+ +SD+
Sbjct: 296  NGNSYQTCDIFIQVQKDLQLSIDLPNVKLCMLGNHQRLNAVTATCTALCLRSQGWAISDE 355

Query: 899  SIRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLL 720
            SIRAGLE+T+LLGR Q LTQ EA A+G+S   IL+DGAHTEASA+GL DVI++ +PDG L
Sbjct: 356  SIRAGLERTQLLGRSQFLTQDEASAIGLSGTSILIDGAHTEASAKGLVDVIQMMHPDGAL 415

Query: 719  ALVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXX 540
            ALVVAMASDKDHLAFA+QLL+G RPEIVLLTEV+IAGGKSRV  ASTLKD W+R      
Sbjct: 416  ALVVAMASDKDHLAFAKQLLSGPRPEIVLLTEVTIAGGKSRVASASTLKDFWIRAALDLG 475

Query: 539  XXXXXXXTIDEERGLHS-LINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNP 363
                    ID  + L       +GS D    +L  C   S+ ++IK A Q+L+SR  + P
Sbjct: 476  IDFVDIGVIDHGKNLEGHKSESRGSSDHSPAMLVVCHIGSVSESIKAASQLLQSRIGNKP 535

Query: 362  RLIVVTGSLHIVSSML 315
             ++V TGSL++VSS+L
Sbjct: 536  SVVVATGSLYVVSSVL 551



 Score =  187 bits (476), Expect(2) = e-177
 Identities = 99/156 (63%), Positives = 112/156 (71%), Gaps = 1/156 (0%)
 Frame = -3

Query: 1911 MLCFTRFISPLQHLVRRTETIALTSRLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGV 1732
            M C  R I    H+   TE   L  R  +SRL S M ED +L DFLDYME LKNYER GV
Sbjct: 1    MQCLARSIFSFCHMRIGTEVSRLAPRFLRSRLQSAMSEDPELGDFLDYMENLKNYERSGV 60

Query: 1731 PKGAGTESDDGFDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSV 1552
            P+GAGT+SDDGFD           GNPHT F+AVH+AGTKGKGSTAAFLSNIL+E GY V
Sbjct: 61   PRGAGTDSDDGFDLGRMRRLLQRLGNPHTQFKAVHIAGTKGKGSTAAFLSNILREEGYLV 120

Query: 1551 GCYTSPHLLTIRERISFGKS-EHVPAEMLMNIFHQV 1447
            GCYTSPHLLTIRERISFG++   V AE+L N+FH+V
Sbjct: 121  GCYTSPHLLTIRERISFGRNGVPVSAEVLKNVFHRV 156


>ref|XP_009421246.1| PREDICTED: probable dihydrofolate synthetase [Musa acuminata subsp.
            malaccensis]
          Length = 555

 Score =  480 bits (1236), Expect(2) = e-173
 Identities = 246/380 (64%), Positives = 306/380 (80%), Gaps = 2/380 (0%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            VFTALA+++FS+++VDIA+IEAGLGGARDATNV+ S ELA SVITS+GEEHLAALGGSLE
Sbjct: 177  VFTALAYLLFSEERVDIAVIEAGLGGARDATNVICSTELATSVITSIGEEHLAALGGSLE 236

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAKSGIIKHG P+VIGGPFE HIE IIR KA  M S V+SACDPGI+  +K+   ED
Sbjct: 237  SIAMAKSGIIKHGCPVVIGGPFESHIECIIRHKASLMKSMVISACDPGIQKTLKWLRMED 296

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            GKP QTCD++I++ EDL++FI +PN+N+ +LG+HQLQNA TATCTALCL  QG+++S+KS
Sbjct: 297  GKPSQTCDIVIQL-EDLQMFITVPNVNLHLLGAHQLQNAATATCTALCLHDQGWVISEKS 355

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IRAGLE+TRLLGR Q LTQ E  ++G+S   IL+DGAHTEASA+GL++VIR+  PDG+LA
Sbjct: 356  IRAGLEKTRLLGRSQFLTQMEVSSIGLSGTTILIDGAHTEASAKGLAEVIRMVQPDGVLA 415

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
             +VAMASDKDHLAFARQLL+G RPE++LLTEVSIAGG+SR+  ASTLK+ W +       
Sbjct: 416  FLVAMASDKDHLAFARQLLSGRRPELILLTEVSIAGGRSRIALASTLKEAWTKALLESGA 475

Query: 536  XXXXXXTIDEERGL--HSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNP 363
                   +D+E+ +  H   +   S   E M LA+C N SI D++K+ADQ+LKSR RD P
Sbjct: 476  DLADVGIMDDEKAIEDHDSCSLITSDPNEVM-LASCENASIVDSVKLADQLLKSRSRDQP 534

Query: 362  RLIVVTGSLHIVSSMLADIH 303
             LIVVTGSLH+VSS+LA +H
Sbjct: 535  ALIVVTGSLHVVSSLLAALH 554



 Score =  159 bits (402), Expect(2) = e-173
 Identities = 81/139 (58%), Positives = 104/139 (74%), Gaps = 1/139 (0%)
 Frame = -3

Query: 1860 TETIALTSRLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGVPKGAGTESDDGFDXXXX 1681
            + + +++SR   S   + +PE+ +L +FLDYMEKLKNYER+GVP GAGT++DDGFD    
Sbjct: 22   SRSASISSRFKSS---AAVPEEPELGEFLDYMEKLKNYERVGVPLGAGTDTDDGFDLGRM 78

Query: 1680 XXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVGCYTSPHLLTIRERISF 1501
                   GNPH  F+AVH+AGTKGKGSTAAF+SNIL+E GYSVGCYTSPHLL+IRERIS 
Sbjct: 79   RRLLQRLGNPHKQFKAVHIAGTKGKGSTAAFISNILREEGYSVGCYTSPHLLSIRERISV 138

Query: 1500 GK-SEHVPAEMLMNIFHQV 1447
            G+  + V A +L  +F +V
Sbjct: 139  GRGGDPVSAGLLKKVFCEV 157


>ref|XP_010249972.1| PREDICTED: probable dihydrofolate synthetase isoform X2 [Nelumbo
            nucifera]
          Length = 555

 Score =  414 bits (1064), Expect(2) = e-152
 Identities = 219/378 (57%), Positives = 282/378 (74%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            VFTALAF +F+Q+KVDIA++EAGLGGARDATNV  S+ LAA++IT++GEEHLAALGGSLE
Sbjct: 179  VFTALAFTLFAQEKVDIAVVEAGLGGARDATNVFCSSGLAAAIITTIGEEHLAALGGSLE 238

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAKSGIIKHG PLV+GGPF  HIE I+RDKA SM+SPVVSA DPG  S +K F    
Sbjct: 239  SIAMAKSGIIKHGCPLVLGGPFNPHIECILRDKASSMDSPVVSAFDPGNLSRIKGFEINK 298

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
             KP Q CD+++++++D++LFI+L ++ + MLG+HQLQNAVTATC ALCLR+QG+ +SD+S
Sbjct: 299  EKPCQLCDIVVQIEKDMQLFIELYDVRLHMLGTHQLQNAVTATCAALCLRNQGWRISDES 358

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            I AGL+ T L GR Q L  KE+E+LG+  A IL+DGAHT+ SA+ L+D I++TYPD    
Sbjct: 359  IHAGLQHTFLPGRSQFLASKESESLGIYGATILLDGAHTKESAKSLADTIQMTYPDAPAV 418

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            LVVAMASDKDHLAFAR+ LTG +PE V+LT+V IAG KSR+T AS LK+ W++       
Sbjct: 419  LVVAMASDKDHLAFAREFLTGMQPEAVILTQVKIAGAKSRMTSASLLKETWIQAFMELGI 478

Query: 536  XXXXXXTIDEERGLHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNPRL 357
                       + +    +      K+  ILA     SI D++K A+ +LK R+ + P +
Sbjct: 479  GFSDIGIGSYSKFVEDEPDCSAGAWKQKTILAI--GDSIEDSLKTANGILKGRKGNRPGI 536

Query: 356  IVVTGSLHIVSSMLADIH 303
            IVVTGSLHIVSS+LA +H
Sbjct: 537  IVVTGSLHIVSSVLAHLH 554



 Score =  154 bits (388), Expect(2) = e-152
 Identities = 83/143 (58%), Positives = 102/143 (71%), Gaps = 5/143 (3%)
 Frame = -3

Query: 1866 RRTETIALTS----RLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGVPKGAGTESDDG 1699
            RR   I+ T+    +L   R  S   ED +L +FLDY+E LKNYE+ GVPKGAGT+S+DG
Sbjct: 15   RRKALISATTGAWKKLGFRRGVSTYSEDLELKEFLDYLENLKNYEKSGVPKGAGTDSEDG 74

Query: 1698 FDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVGCYTSPHLLTI 1519
            FD           GNP + F+AVH+AGTKGKGSTAAFLSN+L+E GYSVGCYTSPHL +I
Sbjct: 75   FDLGRMNRLMERLGNPQSKFKAVHIAGTKGKGSTAAFLSNMLREEGYSVGCYTSPHLWSI 134

Query: 1518 RERISFG-KSEHVPAEMLMNIFH 1453
            RERIS G KS+ V A+ L ++FH
Sbjct: 135  RERISVGRKSDPVSAKALNSVFH 157


>ref|XP_010249889.1| PREDICTED: probable dihydrofolate synthetase isoform X1 [Nelumbo
            nucifera]
          Length = 558

 Score =  407 bits (1046), Expect(2) = e-150
 Identities = 218/381 (57%), Positives = 281/381 (73%), Gaps = 3/381 (0%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            VFTALAF +F+Q+KVDIA++EAGLGGARDATNV  S+ LAA++IT++GEEHLAALGGSLE
Sbjct: 179  VFTALAFTLFAQEKVDIAVVEAGLGGARDATNVFCSSGLAAAIITTIGEEHLAALGGSLE 238

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAKSGIIKHG PLV+GGPF  HIE I+RDKA SM+SPVVSA DPG  S +K F    
Sbjct: 239  SIAMAKSGIIKHGCPLVLGGPFNPHIECILRDKASSMDSPVVSAFDPGNLSRIKGFEINK 298

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
             KP Q CD+++++++D++LFI+L ++ + MLG+HQLQNAVTATC ALCLR+QG+ +SD+S
Sbjct: 299  EKPCQLCDIVVQIEKDMQLFIELYDVRLHMLGTHQLQNAVTATCAALCLRNQGWRISDES 358

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDG---AHTEASARGLSDVIRLTYPDG 726
            I AGL+ T L GR Q L  KE+E+LG+  A IL+DG    HT+ SA+ L+D I++TYPD 
Sbjct: 359  IHAGLQHTFLPGRSQFLASKESESLGIYGATILLDGGLLTHTKESAKSLADTIQMTYPDA 418

Query: 725  LLALVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXX 546
               LVVAMASDKDHLAFAR+ LTG +PE V+LT+V IAG KSR+T AS LK+ W++    
Sbjct: 419  PAVLVVAMASDKDHLAFAREFLTGMQPEAVILTQVKIAGAKSRMTSASLLKETWIQAFME 478

Query: 545  XXXXXXXXXTIDEERGLHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDN 366
                          + +    +      K+  ILA     SI D++K A+ +LK R+ + 
Sbjct: 479  LGIGFSDIGIGSYSKFVEDEPDCSAGAWKQKTILAI--GDSIEDSLKTANGILKGRKGNR 536

Query: 365  PRLIVVTGSLHIVSSMLADIH 303
            P +IVVTGSLHIVSS+LA +H
Sbjct: 537  PGIIVVTGSLHIVSSVLAHLH 557



 Score =  154 bits (388), Expect(2) = e-150
 Identities = 83/143 (58%), Positives = 102/143 (71%), Gaps = 5/143 (3%)
 Frame = -3

Query: 1866 RRTETIALTS----RLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGVPKGAGTESDDG 1699
            RR   I+ T+    +L   R  S   ED +L +FLDY+E LKNYE+ GVPKGAGT+S+DG
Sbjct: 15   RRKALISATTGAWKKLGFRRGVSTYSEDLELKEFLDYLENLKNYEKSGVPKGAGTDSEDG 74

Query: 1698 FDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVGCYTSPHLLTI 1519
            FD           GNP + F+AVH+AGTKGKGSTAAFLSN+L+E GYSVGCYTSPHL +I
Sbjct: 75   FDLGRMNRLMERLGNPQSKFKAVHIAGTKGKGSTAAFLSNMLREEGYSVGCYTSPHLWSI 134

Query: 1518 RERISFG-KSEHVPAEMLMNIFH 1453
            RERIS G KS+ V A+ L ++FH
Sbjct: 135  RERISVGRKSDPVSAKALNSVFH 157


>ref|XP_007038953.1| Folylpolyglutamate synthetase family protein isoform 2 [Theobroma
            cacao] gi|508776198|gb|EOY23454.1| Folylpolyglutamate
            synthetase family protein isoform 2 [Theobroma cacao]
          Length = 549

 Score =  412 bits (1060), Expect(2) = e-148
 Identities = 220/379 (58%), Positives = 283/379 (74%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            V TA+AF +F+Q+ VDIAIIEAGLGGARDATN++ S+ELAAS+IT++GEEHLAALGGSLE
Sbjct: 173  VLTAVAFTLFAQENVDIAIIEAGLGGARDATNIISSSELAASIITTIGEEHLAALGGSLE 232

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAK+GIIKHGRPL++GGPF  HI+ I+RDKA SM+SP+VSA D GIR+A+K   T  
Sbjct: 233  SIAMAKAGIIKHGRPLILGGPFLPHIDCILRDKALSMSSPIVSASDSGIRTAIKGVSTFK 292

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            G+P Q+CD++I++  D +L I+L ++N+ MLG+HQLQNAVTA CTALCL +QG+ +SD S
Sbjct: 293  GRPSQSCDLMIQLDCDFQLSIELCDLNLSMLGTHQLQNAVTAACTALCLCNQGWKISDGS 352

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IRAGLE T L GR Q LT KEAE LG+  A +L+DGAHT+ SA+ L D I++T+PD  LA
Sbjct: 353  IRAGLENTCLQGRSQFLTSKEAETLGLPGATVLIDGAHTKESAKALLDTIQMTFPDSRLA 412

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            +VVAMA DKDHLAFA++LL+G + E V LTE +IAGG SR T AS L+D WM+       
Sbjct: 413  IVVAMACDKDHLAFAKELLSGRQVEAVFLTESNIAGGTSRTTSASVLRDCWMQ-ASRELG 471

Query: 536  XXXXXXTIDEERGLHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNPRL 357
                   I E R L        +RD    IL A  N S+ D+++ A+Q+L+ R  +   +
Sbjct: 472  IKVLHDRIAEYRELFEDKYICSTRDLNHEILVATEN-SLSDSLRFANQILRERTWNRSGI 530

Query: 356  IVVTGSLHIVSSMLADIHR 300
            IVVTGSLHIVS +LA ++R
Sbjct: 531  IVVTGSLHIVSLVLASLNR 549



 Score =  142 bits (359), Expect(2) = e-148
 Identities = 75/148 (50%), Positives = 100/148 (67%), Gaps = 6/148 (4%)
 Frame = -3

Query: 1872 LVRRTETIALTSRLSKSRL-----CSIMPEDNKLSDFLDYMEKLKNYERLGVPKGAGTES 1708
            LV +  T+     L K RL      S   E+ +L DF+ Y++ LKNYE+ GVPK AGT+S
Sbjct: 6    LVSQLSTLTCRKPLKKFRLESKQWFSTYTEEPELKDFIQYIDSLKNYEKSGVPKDAGTDS 65

Query: 1707 DDGFDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVGCYTSPHL 1528
            DDGFD           GNP ++F++VH+AGTKGKGSTAA+LSNIL+  GYSVGCYTSPH+
Sbjct: 66   DDGFDLGRMRRLMDRLGNPQSNFKSVHIAGTKGKGSTAAYLSNILRTEGYSVGCYTSPHI 125

Query: 1527 LTIRERISFGK-SEHVPAEMLMNIFHQV 1447
            L+IRER+S G+  + V +  L  +FH++
Sbjct: 126  LSIRERMSVGRLGKPVSSNTLNCLFHRI 153


>ref|XP_004958237.1| PREDICTED: probable dihydrofolate synthetase [Setaria italica]
          Length = 559

 Score =  397 bits (1020), Expect(2) = e-146
 Identities = 214/388 (55%), Positives = 282/388 (72%), Gaps = 9/388 (2%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            VFTAL+F++FSQ+ VDIAI+EAGLGGARDATNV++S ELAASVIT+VG EHLAALGGSL+
Sbjct: 171  VFTALSFLLFSQENVDIAIVEAGLGGARDATNVIQSTELAASVITTVGREHLAALGGSLQ 230

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            +IA+AKSGIIK GRP+VIGGPF   IE+IIRD+AF   SPV+SACDPGI+S  K    ++
Sbjct: 231  NIAIAKSGIIKQGRPVVIGGPFSTDIEQIIRDRAFLTQSPVISACDPGIKSISKCIYWDN 290

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            GKPYQ CD+ I++  D    I+L ++N+Q+LG HQ QNAVTA+CTALCLR+ G+ +SD S
Sbjct: 291  GKPYQRCDISIKISNDTLFSIELHDVNLQLLGDHQRQNAVTASCTALCLRNLGWDISDAS 350

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGV-SEAVILVDGAHTEASARGLSDVIRLTYPDGLL 720
            I++GLE+T+L GR Q LT++EA  LG+   + IL+DGAHTEASA+ LS+VI    P+G L
Sbjct: 351  IQSGLEETQLPGRSQFLTREEALVLGLDGPSTILIDGAHTEASAKALSNVIETVRPEGPL 410

Query: 719  ALVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXX 540
            ALVV MA+DK+HLAFA QLL G+RP +VLLTE SIAGG SR  PA +LK++WM       
Sbjct: 411  ALVVGMANDKEHLAFAEQLLAGSRPNVVLLTEASIAGGASRAMPAPSLKEIWMAAARDLG 470

Query: 539  XXXXXXXTIDEERGLHSLINFKGSRDK---EFMILAACRNLSI---CDTIKVADQ-MLKS 381
                   TI   +    + N   S      +  ++  C++ ++   C+ I+ A Q +L+S
Sbjct: 471  INCVDIGTISGAKAPEHIANLAASPSSLAGKPTVMIGCQDAAVPFSCNLIRAASQIILES 530

Query: 380  RERD-NPRLIVVTGSLHIVSSMLADIHR 300
            R    +P L+ VTGSLH+V+S+L  + R
Sbjct: 531  RGGGASPGLVCVTGSLHVVASVLQHLER 558



 Score =  152 bits (384), Expect(2) = e-146
 Identities = 74/117 (63%), Positives = 90/117 (76%), Gaps = 1/117 (0%)
 Frame = -3

Query: 1797 DNKLSDFLDYMEKLKNYERLGVPKGAGTESDDGFDXXXXXXXXXXXGNPHTHFQAVHVAG 1618
            + +L DF +YME+L+NYER GVP+GAGT+SDDGFD           G+PHTHF AVH+AG
Sbjct: 34   EGQLGDFFEYMERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRRLGDPHTHFPAVHIAG 93

Query: 1617 TKGKGSTAAFLSNILQEGGYSVGCYTSPHLLTIRERISFGK-SEHVPAEMLMNIFHQ 1450
            TKGKGSTAAFLSNI++E GY+VGCY+SPHLLTIRERIS G     VP  +L ++F Q
Sbjct: 94   TKGKGSTAAFLSNIMREQGYNVGCYSSPHLLTIRERISVGNDGGPVPVGLLSDLFDQ 150


>ref|XP_009357329.1| PREDICTED: probable dihydrofolate synthetase [Pyrus x bretschneideri]
          Length = 555

 Score =  400 bits (1029), Expect(2) = e-146
 Identities = 210/379 (55%), Positives = 279/379 (73%), Gaps = 1/379 (0%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            V TALAF +F+Q+ VDIA+IEAGLGGARDATNV+ S+ELAAS+IT++GEEHLAALGGSLE
Sbjct: 180  VLTALAFTLFAQENVDIAVIEAGLGGARDATNVICSSELAASIITTIGEEHLAALGGSLE 239

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAKSGIIKHGRP+V+GGPF  HI++I+RDKA  M+SP+V A D G +S +      +
Sbjct: 240  SIAMAKSGIIKHGRPVVLGGPFLPHIDQILRDKALLMSSPMVLASDTGNKSKINGVSMLN 299

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            G+P+Q+CD++IRV+ DL+LFI+L ++ + MLG+HQLQNA TATC ALCLR+ G+ +SD S
Sbjct: 300  GRPFQSCDIVIRVERDLQLFIELLDVKLYMLGTHQLQNASTATCAALCLRNLGWRISDGS 359

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IR GLEQT LLGR Q LT KEA+ALG+S A IL+DGAHT+ SAR L D +++T+P+  L 
Sbjct: 360  IRTGLEQTSLLGRSQFLTSKEAQALGLSGATILLDGAHTKDSARALMDTMKMTFPEAGLT 419

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            LVVAMASDKDHL FA++ L+G   E V+LTE +IAG KSR T AS L+D W++       
Sbjct: 420  LVVAMASDKDHLGFAKECLSGGHVEGVILTEANIAGAKSRTTAASLLRDCWIQASQELET 479

Query: 536  XXXXXXTIDEERG-LHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNPR 360
                    + +   +  L+    S ++  +        S   ++K+A+Q++K R  D  R
Sbjct: 480  DILHDEMPEHQNSTMDQLLCISRSGNRPILSTEG----SFQASLKIANQIIKGRTGDRLR 535

Query: 359  LIVVTGSLHIVSSMLADIH 303
            ++V+TGSLHIVS +LA +H
Sbjct: 536  IVVITGSLHIVSLVLATLH 554



 Score =  147 bits (372), Expect(2) = e-146
 Identities = 74/136 (54%), Positives = 96/136 (70%), Gaps = 1/136 (0%)
 Frame = -3

Query: 1851 IALTSRLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGVPKGAGTESDDGFDXXXXXXX 1672
            + L   +   R      ED++L   L+YM+ LKNYE+LGVPK AGT+S+DGFD       
Sbjct: 25   VGLLRNVGPERCFCTRSEDSELKGLLEYMDSLKNYEKLGVPKDAGTDSEDGFDLGRMRRL 84

Query: 1671 XXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVGCYTSPHLLTIRERISFGK- 1495
                GNPH+ F+AVH+AGTKGKGSTAAFLSN+L+  GYS+GCYTSPH+ TIRERIS G+ 
Sbjct: 85   LELLGNPHSKFKAVHIAGTKGKGSTAAFLSNMLRAEGYSIGCYTSPHIQTIRERISVGRF 144

Query: 1494 SEHVPAEMLMNIFHQV 1447
            +E V A+ L ++F +V
Sbjct: 145  AEPVSAKALSSLFDRV 160


>gb|ABA99426.2| dihydrofolate synthetase/folylpolyglutamate synthetase, putative,
            expressed [Oryza sativa Japonica Group]
            gi|218187281|gb|EEC69708.1| hypothetical protein
            OsI_39178 [Oryza sativa Indica Group]
            gi|222617510|gb|EEE53642.1| hypothetical protein
            OsJ_36925 [Oryza sativa Japonica Group]
          Length = 510

 Score =  402 bits (1034), Expect(2) = e-145
 Identities = 218/387 (56%), Positives = 285/387 (73%), Gaps = 8/387 (2%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            VFTAL++++FSQ+ VDIAI+EAGLGGARDATNV++S ELAASVIT+VG+EHLAALGGSL+
Sbjct: 128  VFTALSYLLFSQENVDIAIVEAGLGGARDATNVIQSTELAASVITTVGKEHLAALGGSLQ 187

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIA+AKSGIIK  RP++IGGPF   IE+I+RD+AF   SPV+SACDPGIRS  +  G + 
Sbjct: 188  SIAVAKSGIIKQERPVIIGGPFSPDIEQILRDRAFLTQSPVISACDPGIRSITRCIGWDC 247

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            GKPYQ+CD++I +  D+ LFI+L ++ +Q+LG HQ QNAVTA CTALCLR  G+ +SD S
Sbjct: 248  GKPYQSCDIVIEISNDIPLFIELQDLKLQLLGDHQRQNAVTACCTALCLRELGWNISDTS 307

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGV-SEAVILVDGAHTEASARGLSDVIRLTYPDGLL 720
            I+AGLE+T+L GR Q LT++E   LG+   + +LVDGAHTEASA+ LSDVI+   P G L
Sbjct: 308  IQAGLEKTQLPGRSQFLTKEETSVLGLDGTSSVLVDGAHTEASAKALSDVIKTVQPRGPL 367

Query: 719  ALVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXX 540
            ALVV MA+DK+HLAFA QLL+G RP++VLLTE SIAGG+SR  PAS+LK++WM       
Sbjct: 368  ALVVGMANDKEHLAFAEQLLSGPRPDVVLLTEASIAGGRSRSMPASSLKEIWM--AAAQS 425

Query: 539  XXXXXXXTIDEERGLHSLI-------NFKGSRDKEFMILAACRNLSICDTIKVADQMLKS 381
                    I    G+ + I       +   S DK  M++  C   S  D I+VA ++L++
Sbjct: 426  QGISCYSDIGTITGVEAPIIASRPTTSRSLSGDKP-MLIGCCTPFS-SDLIRVASRLLQT 483

Query: 380  RERDNPRLIVVTGSLHIVSSMLADIHR 300
            +E  +  LI VTGSLH+VSS+L  + +
Sbjct: 484  QEDGDTGLICVTGSLHMVSSVLGQLRQ 510



 Score =  144 bits (364), Expect(2) = e-145
 Identities = 72/105 (68%), Positives = 85/105 (80%), Gaps = 1/105 (0%)
 Frame = -3

Query: 1767 MEKLKNYERLGVPKGAGTESDDGFDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAF 1588
            ME+L+NYER GVP+GAGT+SDDGFD           G+PHTHF AVH+AGTKGKGSTAAF
Sbjct: 1    MERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRRLGDPHTHFPAVHIAGTKGKGSTAAF 60

Query: 1587 LSNILQEGGYSVGCYTSPHLLTIRERISFGKS-EHVPAEMLMNIF 1456
            LSNI++E GY+VGCYTSPHLLTIRERIS G+S   V AE+L ++F
Sbjct: 61   LSNIMREQGYNVGCYTSPHLLTIRERISVGQSGGPVSAELLRDLF 105


>ref|XP_009368683.1| PREDICTED: probable dihydrofolate synthetase [Pyrus x bretschneideri]
          Length = 555

 Score =  397 bits (1021), Expect(2) = e-145
 Identities = 209/379 (55%), Positives = 277/379 (73%), Gaps = 1/379 (0%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            V TALAF +F+Q+ VDIA+IEAGLGGARDATNV+ S+ELAAS+IT++GEEHLAALGGSLE
Sbjct: 180  VLTALAFTLFAQENVDIAVIEAGLGGARDATNVICSSELAASIITTIGEEHLAALGGSLE 239

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAKSGIIKHGRP+V+GGPF  HI++I+RDKA  M+S +V A D G +S +      +
Sbjct: 240  SIAMAKSGIIKHGRPVVLGGPFLPHIDQILRDKALLMSSLMVLASDTGNKSKINGVSMLN 299

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            G+P+Q+CD++IRV+ DL+LFI+L ++ + MLG+HQLQNA TATC ALCLR+ G+ +SD S
Sbjct: 300  GRPFQSCDIVIRVERDLQLFIELLDVKLYMLGTHQLQNASTATCAALCLRNLGWRISDGS 359

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IR GLEQT LLGR Q LT KEA+ALG+S A IL+DGAHT+ SAR L D I++T+P+  L 
Sbjct: 360  IRTGLEQTSLLGRSQFLTSKEAQALGLSGATILLDGAHTKDSARALMDTIKMTFPEAGLT 419

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            LVVAMASDKDHL FA++ L+G   E V+LTE +IAG KSR T AS L+D W++       
Sbjct: 420  LVVAMASDKDHLGFAKECLSGGHVECVILTEANIAGAKSRTTAASLLRDCWIQASQELET 479

Query: 536  XXXXXXTIDEERG-LHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNPR 360
                    + +   +  L+    S ++  +        S   ++K+ +Q++K R  D  R
Sbjct: 480  DILHDKMPEHQNSTMDQLLCISRSGNRPILSTEG----SFQASLKIVNQIIKGRTGDRLR 535

Query: 359  LIVVTGSLHIVSSMLADIH 303
            ++V+TGSLHIVS +LA +H
Sbjct: 536  IVVITGSLHIVSLVLATLH 554



 Score =  148 bits (374), Expect(2) = e-145
 Identities = 75/136 (55%), Positives = 96/136 (70%), Gaps = 1/136 (0%)
 Frame = -3

Query: 1851 IALTSRLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGVPKGAGTESDDGFDXXXXXXX 1672
            + L   +   R      ED++L   L+YM+ LKNYE+LGVPK AGT+S+DGFD       
Sbjct: 25   VGLLRNVGPERCLCTRSEDSELKGLLEYMDSLKNYEKLGVPKDAGTDSEDGFDLGRMRRL 84

Query: 1671 XXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVGCYTSPHLLTIRERISFGK- 1495
                GNPH+ F+AVH+AGTKGKGSTAAFLSN+L+  GYSVGCYTSPH+ TIRERIS G+ 
Sbjct: 85   LELLGNPHSKFKAVHIAGTKGKGSTAAFLSNMLRAEGYSVGCYTSPHIQTIRERISVGRF 144

Query: 1494 SEHVPAEMLMNIFHQV 1447
            +E V A+ L ++F +V
Sbjct: 145  AEPVSAKALSSLFDRV 160


>ref|XP_003577221.1| PREDICTED: probable dihydrofolate synthetase [Brachypodium
            distachyon]
          Length = 563

 Score =  387 bits (995), Expect(2) = e-145
 Identities = 212/391 (54%), Positives = 277/391 (70%), Gaps = 12/391 (3%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            VFT L+F++FS++ VD AI+EAGLGGARDATNV+R+ ELAASVIT+VG+EHLAALGGSL+
Sbjct: 174  VFTTLSFLLFSRENVDFAIVEAGLGGARDATNVIRNTELAASVITTVGKEHLAALGGSLQ 233

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIA+AKSGIIK G+P++IGGPF   IE+IIRD+AF   S V+SACDPG+RS  +  G + 
Sbjct: 234  SIAVAKSGIIKEGQPVIIGGPFSTDIEQIIRDRAFLTQSTVISACDPGVRSITRCVGWDY 293

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            GKPYQ+CD+ +++  D+ L ++L +I +Q+LG HQ QNAVTA+CTALCLR  G+ +SD S
Sbjct: 294  GKPYQSCDIAVQISNDMPLSMELHDIKLQLLGDHQRQNAVTASCTALCLRELGWNISDAS 353

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVS-EAVILVDGAHTEASARGLSDVIRLTYPDGLL 720
            IRAGLE+T+LLGR Q LT  EA  LG+   + IL+DGAHTEASA+ LSDVIR   P+G L
Sbjct: 354  IRAGLEKTQLLGRSQFLTHDEASVLGLDRSSTILIDGAHTEASAKALSDVIRTVEPEGPL 413

Query: 719  ALVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXX 540
            AL++ MA+DK+HLAFA QLL+G RP+IVLLTE  IAGG +R   AS+LKD+W        
Sbjct: 414  ALIIGMANDKEHLAFAAQLLSGRRPDIVLLTEARIAGGTARSMLASSLKDIWTGVAHDQG 473

Query: 539  XXXXXXXTI--DEERGLHSLI--NFKGSRDKEFMILAACRNLSIC-DTIKVADQMLKSRE 375
                    +  DE   L   +      S +K   ++  C N     D +K A ++L++R 
Sbjct: 474  IDCTDIGVVTSDESPDLIDRLAAAATSSPNKPAPMMIGCGNAPFSRDLLKAASRLLQARS 533

Query: 374  RDN------PRLIVVTGSLHIVSSMLADIHR 300
             DN        LI VTGSLH+VSS+L  + +
Sbjct: 534  -DNGDVGAPAGLICVTGSLHMVSSVLQQLEQ 563



 Score =  157 bits (398), Expect(2) = e-145
 Identities = 88/156 (56%), Positives = 113/156 (72%), Gaps = 6/156 (3%)
 Frame = -3

Query: 1899 TRFISPLQH---LVRRTETIALTSRLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGVP 1729
            +RF SP++    L+R T  ++ +SR   S +     E+ KL +FL+YM++L+NYER GVP
Sbjct: 3    SRFPSPIRWSRGLLRGT--VSSSSRRGVSAMAR-GGEEGKLGEFLEYMDRLRNYERSGVP 59

Query: 1728 KGAGTESDDGFDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVG 1549
            +GAGT+SDDGFD           G+PHTH+ AVH+AGTKGKGSTAAFLSNIL+E GY+VG
Sbjct: 60   RGAGTDSDDGFDLGRMRRLLRRLGDPHTHYPAVHIAGTKGKGSTAAFLSNILREQGYNVG 119

Query: 1548 CYTSPHLLTIRERISFGKSEH---VPAEMLMNIFHQ 1450
            CY+SPHLLTIRERIS G  EH   VPA +L ++F Q
Sbjct: 120  CYSSPHLLTIRERISVG--EHGGPVPAALLSDLFDQ 153


>ref|XP_008376690.1| PREDICTED: probable dihydrofolate synthetase [Malus domestica]
          Length = 555

 Score =  397 bits (1020), Expect(2) = e-145
 Identities = 209/379 (55%), Positives = 275/379 (72%), Gaps = 1/379 (0%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            V TALAF +F+Q+ VDIA+IEAGLGGARDATNV+ S+ELAASVIT++GEEHLAALGGSLE
Sbjct: 180  VLTALAFTLFAQENVDIAVIEAGLGGARDATNVICSSELAASVITTIGEEHLAALGGSLE 239

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAKSGIIKHG P+V+GGPF  HIE+I+RDKA  M+SPVV A D G +S +      +
Sbjct: 240  SIAMAKSGIIKHGCPVVLGGPFLPHIEQILRDKALLMSSPVVLASDTGNKSKINGVSMLN 299

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            G+P+Q+CD++IRV+ DLKLFI+L ++ + MLG+HQLQNA TATC ALCLR+ G+ +SD S
Sbjct: 300  GRPFQSCDIVIRVERDLKLFIELLDVKLYMLGTHQLQNAATATCAALCLRNLGWRISDGS 359

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IR GLEQTRL GR Q LT KEA+ALG+S   IL+DGAHT+ SAR L D +++T+P+  L 
Sbjct: 360  IRTGLEQTRLFGRSQFLTSKEAQALGLSGTTILLDGAHTKESARALMDTVKMTFPEAGLT 419

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            LVVAMASDKDHL FA++ L+G   E V+LTE +IAG  SR T AS+L+D W++       
Sbjct: 420  LVVAMASDKDHLGFAKECLSGGHVEGVVLTEANIAGATSRTTAASSLRDCWIQASQELET 479

Query: 536  XXXXXXTIDEERG-LHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNPR 360
                    + +   +  L+    S +K  +        S   ++K+ +Q++K R  D  +
Sbjct: 480  DILHDEMPEHQNSTMDQLLCISKSGNKPILSTEG----SFQASLKITNQIIKGRTGDRLQ 535

Query: 359  LIVVTGSLHIVSSMLADIH 303
            ++V+ GSLHIVS +LA +H
Sbjct: 536  IVVIAGSLHIVSLVLATLH 554



 Score =  148 bits (373), Expect(2) = e-145
 Identities = 75/136 (55%), Positives = 96/136 (70%), Gaps = 1/136 (0%)
 Frame = -3

Query: 1851 IALTSRLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGVPKGAGTESDDGFDXXXXXXX 1672
            + L   +   R      ED++L   L+YM+ LKNYE+LGVPK AGT+S+DGFD       
Sbjct: 25   VGLLRNVGPERCFCTRSEDSELKGLLEYMDSLKNYEKLGVPKDAGTDSEDGFDLGRMRRL 84

Query: 1671 XXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVGCYTSPHLLTIRERISFGK- 1495
                GNPH+ F+AVH+AGTKGKGSTAAFLSN+L+  GYSVGCYTSPH+ TIRERIS G+ 
Sbjct: 85   LELLGNPHSKFKAVHIAGTKGKGSTAAFLSNMLRAEGYSVGCYTSPHIQTIRERISVGRF 144

Query: 1494 SEHVPAEMLMNIFHQV 1447
            +E V A+ L ++F +V
Sbjct: 145  AEPVSAKALSSLFDRV 160


>ref|XP_006664219.1| PREDICTED: putative folylpolyglutamate synthase-like [Oryza
            brachyantha]
          Length = 511

 Score =  397 bits (1021), Expect(2) = e-144
 Identities = 212/387 (54%), Positives = 278/387 (71%), Gaps = 8/387 (2%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            VFTAL++++FS++ VDIAI+EAGLGGARDATNV++S ELAASVIT++G+EHLAALGGSL+
Sbjct: 128  VFTALSYLLFSRENVDIAIVEAGLGGARDATNVIQSTELAASVITTIGKEHLAALGGSLQ 187

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIA+AKSGIIK  RP++IGGPF   IE+I+RD+AF   SP++SACDPG+RS  +    + 
Sbjct: 188  SIAVAKSGIIKQERPVIIGGPFSSDIEQILRDRAFLTQSPIISACDPGVRSITRCIRWDC 247

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            GKPYQ+CD++I +  ++ LFI+L ++ IQ+LG HQ QNAVTA CTALCLR  G+ +SD S
Sbjct: 248  GKPYQSCDIIIEISNNIPLFIELHDVKIQLLGDHQRQNAVTACCTALCLRELGWDISDSS 307

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEA-VILVDGAHTEASARGLSDVIRLTYPDGLL 720
            IRAGLE+T+L GR Q LT++E   LG+  A  +LVDGAHTEASA+ LSDVI+   P G L
Sbjct: 308  IRAGLEKTQLPGRSQFLTEEETSVLGLDGASSVLVDGAHTEASAKALSDVIKTVKPRGPL 367

Query: 719  ALVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXX 540
            A VV MA+DK+H AFA QLL+G RP+IVLLTE SIAGG+SR  PAS+LK+VWM       
Sbjct: 368  AFVVGMANDKEHFAFAEQLLSGPRPDIVLLTEASIAGGRSRSMPASSLKEVWMTAAHSQG 427

Query: 539  XXXXXXXTIDEERGLHSLI------NFKGSRDKEFMILAACRNLSI-CDTIKVADQMLKS 381
                    I    G+ + I         GS   +  +L  C +    CD I+VA ++L+ 
Sbjct: 428  ISYSDIGMI---TGIEAPIIASRRPAATGSSSSDTPMLIGCSSAPFSCDLIRVASRLLQG 484

Query: 380  RERDNPRLIVVTGSLHIVSSMLADIHR 300
            +   +  LI V GSLH+VSS+L  + +
Sbjct: 485  QADGDTGLICVAGSLHMVSSVLGQLRQ 511



 Score =  144 bits (364), Expect(2) = e-144
 Identities = 73/105 (69%), Positives = 84/105 (80%), Gaps = 1/105 (0%)
 Frame = -3

Query: 1767 MEKLKNYERLGVPKGAGTESDDGFDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAF 1588
            ME+L+NYER GVP+GAGT+SDDGFD           G+PHTHF AVH+AGTKGKGSTAAF
Sbjct: 1    MERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRCLGDPHTHFPAVHIAGTKGKGSTAAF 60

Query: 1587 LSNILQEGGYSVGCYTSPHLLTIRERISFGKS-EHVPAEMLMNIF 1456
            LSNI++E GY+VGCYTSPHLLTIRERIS G+S   V A +L NIF
Sbjct: 61   LSNIMREQGYNVGCYTSPHLLTIRERISVGESGGPVSAAVLRNIF 105


>ref|XP_002442305.1| hypothetical protein SORBIDRAFT_08g017730 [Sorghum bicolor]
            gi|241942998|gb|EES16143.1| hypothetical protein
            SORBIDRAFT_08g017730 [Sorghum bicolor]
          Length = 567

 Score =  391 bits (1005), Expect(2) = e-143
 Identities = 213/387 (55%), Positives = 272/387 (70%), Gaps = 8/387 (2%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            VFTAL+F++FSQ+ VDIAIIEAGLGGARDATNV+RS ELAASVIT+VG EHLAALGGSL+
Sbjct: 180  VFTALSFLLFSQENVDIAIIEAGLGGARDATNVIRSTELAASVITTVGREHLAALGGSLQ 239

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIA+AKSGIIK GRP+VIGGPF   IE+IIRD+AF   SPV+SACDPG++S  K    ++
Sbjct: 240  SIAIAKSGIIKQGRPVVIGGPFAADIEQIIRDRAFLTQSPVISACDPGVKSFTKCIDWDN 299

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            GKPYQ C + I++  D+ L I++ +IN+Q+LG HQ QNAVTA C ALCLR +G+ +SD S
Sbjct: 300  GKPYQRCYINIKISNDVPLSIEMQDINLQLLGDHQRQNAVTAACAALCLRSEGWDISDAS 359

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEA-VILVDGAHTEASARGLSDVIRLTYPDGLL 720
            I+AGLE+T+L GR Q LT +EA  LG++ A  +L+DGAHTE SA+ LS VI+   P+G L
Sbjct: 360  IQAGLEETQLPGRSQFLTAEEASVLGLNGASTVLIDGAHTEESAKALSGVIKTVRPEGPL 419

Query: 719  ALVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXX 540
            ALVV MA+DK+HLAFA QLL+G  P++VLLTE SIAGG SR  PAS+LK+VW+       
Sbjct: 420  ALVVGMATDKEHLAFAEQLLSGQTPDVVLLTEASIAGGTSRAMPASSLKEVWIAAARDHG 479

Query: 539  XXXXXXXTIDEERGLHSLINFKGSRD-----KEFMILAACRNLSICDTIKVADQML--KS 381
                    +        + +  GS       K  M++           + +A   L  KS
Sbjct: 480  IECVDIGGVSGAEAPEHIGDLLGSPSSSSGRKPPMVIGCQDEGPFSSNLIIAASRLLEKS 539

Query: 380  RERDNPRLIVVTGSLHIVSSMLADIHR 300
            R R +P LI VTGSLH+V ++L  + R
Sbjct: 540  RGRAHPGLICVTGSLHLVGAVLQQLGR 566



 Score =  149 bits (375), Expect(2) = e-143
 Identities = 73/118 (61%), Positives = 89/118 (75%), Gaps = 1/118 (0%)
 Frame = -3

Query: 1800 EDNKLSDFLDYMEKLKNYERLGVPKGAGTESDDGFDXXXXXXXXXXXGNPHTHFQAVHVA 1621
            E+  L  FL+YME+L+NYER GVP+GAGT+SDDGFD           G+PHTHF AVH+A
Sbjct: 42   EEGPLGGFLEYMERLRNYERSGVPRGAGTDSDDGFDLGRMRRLLRRLGDPHTHFPAVHIA 101

Query: 1620 GTKGKGSTAAFLSNILQEGGYSVGCYTSPHLLTIRERISFGK-SEHVPAEMLMNIFHQ 1450
            GTKGKGSTAAFLSNI++  GY+VGCY+SPHLLTIRERIS G     VP  +L ++F +
Sbjct: 102  GTKGKGSTAAFLSNIMRAQGYNVGCYSSPHLLTIRERISVGNDGGPVPVGLLSDLFDE 159


>ref|XP_002267695.2| PREDICTED: probable dihydrofolate synthetase isoform X2 [Vitis
            vinifera] gi|302143688|emb|CBI22549.3| unnamed protein
            product [Vitis vinifera]
          Length = 536

 Score =  399 bits (1026), Expect(2) = e-143
 Identities = 210/377 (55%), Positives = 278/377 (73%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            + TA+AF +F+Q+ VD+A+IEAGLGGARDATN++ S+ LAA+VIT++GEEHLAALGGSLE
Sbjct: 160  ILTAMAFKLFAQENVDVAVIEAGLGGARDATNIISSSGLAAAVITTIGEEHLAALGGSLE 219

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAKSGIIK G PLV+GGPF  HIE I  DKA SM SPVVSA  PG RSAVK     +
Sbjct: 220  SIAMAKSGIIKQGCPLVLGGPFLPHIEHIFLDKASSMCSPVVSASGPGNRSAVKGVSKSN 279

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            GKP+Q+CD++I V+ D KLFI+L ++ +QMLG HQLQNA TATC ALCLR +G+ +SD+S
Sbjct: 280  GKPFQSCDIVIEVERDFKLFIELFDVKLQMLGIHQLQNAATATCVALCLRDKGWRISDES 339

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            I AGLE   LLGR Q LT  EAE LG+  A I++DGAHT+ SA+ L D I++T+P+  LA
Sbjct: 340  IHAGLEHAYLLGRSQFLTSTEAETLGLPGATIMLDGAHTKESAKALVDTIQMTFPEARLA 399

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            L+VAMASDKDH+AFAR+ L+G + E V LTEV+IAG KSR T AS L+D W++       
Sbjct: 400  LIVAMASDKDHMAFAREFLSGGQLEAVFLTEVNIAGAKSRTTSASMLRDCWIQ-ASKELG 458

Query: 536  XXXXXXTIDEERGLHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNPRL 357
                   ++E + L    +F  + + +   + A  N S+  +++V +Q+L++R RD   +
Sbjct: 459  INTLHDGMEEYQKLFENQSFCSAGESKHKTILAAEN-SLLVSLRVGNQILRARTRDQTSI 517

Query: 356  IVVTGSLHIVSSMLADI 306
            IV+TGSLHIVS++L+ +
Sbjct: 518  IVITGSLHIVSTVLSSL 534



 Score =  139 bits (350), Expect(2) = e-143
 Identities = 70/116 (60%), Positives = 86/116 (74%), Gaps = 1/116 (0%)
 Frame = -3

Query: 1791 KLSDFLDYMEKLKNYERLGVPKGAGTESDDGFDXXXXXXXXXXXGNPHTHFQAVHVAGTK 1612
            +L DFL+Y++ LKNYE+ GVPK AGT+S  GFD           GNP T F+AVH+AGTK
Sbjct: 25   ELKDFLNYLDNLKNYEKCGVPKDAGTDSSHGFDLGRMNRLMDRLGNPETGFKAVHIAGTK 84

Query: 1611 GKGSTAAFLSNILQEGGYSVGCYTSPHLLTIRERISFGK-SEHVPAEMLMNIFHQV 1447
            GKGSTAAFL+NIL+  GY+VGCYTSPH+ TIRERIS GK  E V A+ L  +FH++
Sbjct: 85   GKGSTAAFLANILRTEGYAVGCYTSPHVRTIRERISLGKLGEPVSAKALNCLFHRI 140


>ref|XP_012090121.1| PREDICTED: probable folylpolyglutamate synthase [Jatropha curcas]
          Length = 557

 Score =  382 bits (980), Expect(2) = e-142
 Identities = 203/375 (54%), Positives = 262/375 (69%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            V TA+AF +F+Q+ +DIAIIE GLGGARDATN++ S+ LAASVITS+GEEHL ALGGSLE
Sbjct: 181  VLTAIAFSLFAQENIDIAIIETGLGGARDATNIICSSGLAASVITSIGEEHLMALGGSLE 240

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            +IAMAK+GIIKH RP+V+GGPF  HIE I+R+KA  MN+PVVSA D   R+ +K     D
Sbjct: 241  TIAMAKAGIIKHNRPVVLGGPFLPHIEHILREKASQMNAPVVSASDSETRTTIKGLSMCD 300

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            G+  Q CD++IRV  D +LF DLP I + MLGSHQLQNA TA C ALCLR+QG+ +SD+S
Sbjct: 301  GRQCQLCDIIIRVDRDFQLFADLPEIKMCMLGSHQLQNAATAACAALCLRNQGWGISDRS 360

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IR G+E T L GR Q L  KEAEALG+  A +L+DGAHT  SA+ L D I++T+P   LA
Sbjct: 361  IRVGIENTCLPGRSQFLASKEAEALGLPGATVLLDGAHTRESAKALVDTIQMTFPKATLA 420

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            LVVAMASDKDHLAFAR+   G     V LTE  IAGGKSR T AS+L+D W++       
Sbjct: 421  LVVAMASDKDHLAFAREFFLGKNLGAVFLTEADIAGGKSRTTTASSLRDCWVQASEELGI 480

Query: 536  XXXXXXTIDEERGLHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNPRL 357
                    + +      + +     +  ++L A ++L +  +++ A++ LK +  +  R 
Sbjct: 481  NILHDGMTEYQELCTKQLVYSAREMEGRVLLVAEKSLEV--SLRDANEFLKGKTGNESRA 538

Query: 356  IVVTGSLHIVSSMLA 312
            +VVTGSLHIVSS+LA
Sbjct: 539  LVVTGSLHIVSSVLA 553



 Score =  152 bits (384), Expect(2) = e-142
 Identities = 79/158 (50%), Positives = 106/158 (67%), Gaps = 8/158 (5%)
 Frame = -3

Query: 1896 RFISPLQHLVRRTETIALTSR-------LSKSRLCSIMPEDNKLSDFLDYMEKLKNYERL 1738
            +FI  +  L+RR   ++  SR       L  ++L S   E+ +L +F+DY+  LKNYE+ 
Sbjct: 4    KFIRQVPALLRRQRLVSAYSREALSPRGLGFNKLFSTYMEEPELKEFIDYLGSLKNYEKS 63

Query: 1737 GVPKGAGTESDDGFDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGY 1558
            G+PKGAGT+SDDGFD            NP + F+A+H+AGTKGKGSTAAFLSNIL+  GY
Sbjct: 64   GIPKGAGTDSDDGFDLSRMRRLMNHLDNPQSKFKAIHIAGTKGKGSTAAFLSNILRAEGY 123

Query: 1557 SVGCYTSPHLLTIRERISFGK-SEHVPAEMLMNIFHQV 1447
            SVGCYTSPH++T+RER+S GK  E V A+ L   FH++
Sbjct: 124  SVGCYTSPHIVTVRERMSIGKLGEPVSAKALNCHFHRI 161


>ref|XP_008234781.1| PREDICTED: dihydrofolate synthetase [Prunus mume]
          Length = 556

 Score =  389 bits (1000), Expect(2) = e-142
 Identities = 208/379 (54%), Positives = 272/379 (71%), Gaps = 1/379 (0%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            V TA+AF +F+Q+ V IA+IEAGLGGARDATNV+ S+ LA SVIT++GEEHLAALGGSLE
Sbjct: 179  VLTAMAFTLFAQENVGIAVIEAGLGGARDATNVICSSGLATSVITTIGEEHLAALGGSLE 238

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIA AKSGIIKHGRP+V+GGPF   IE I+RDKAF M+SPVV A D G RS +      +
Sbjct: 239  SIATAKSGIIKHGRPVVLGGPFLPRIEHILRDKAFLMSSPVVLASDSGNRSKINGVSMLN 298

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            G+P+Q+CD++IRV+ DLKLFI+L ++ + MLG+HQLQNAVTATC ALCLRH G+ +SD+ 
Sbjct: 299  GRPFQSCDIVIRVERDLKLFIELLDVKLYMLGTHQLQNAVTATCAALCLRHLGWRISDEC 358

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IR GL+QT LLGR Q LT KEA+A+G+S A IL+DGAHT+ SAR L + I++T+P+  L 
Sbjct: 359  IRTGLQQTHLLGRSQFLTSKEAQAIGLSGATILLDGAHTKESARALMETIKMTFPEAGLT 418

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            LVVAMASDKDHL FAR+ L+G     V+L E SIAG KSR T AS L++ W++       
Sbjct: 419  LVVAMASDKDHLGFARECLSGGHVNGVVLMEASIAGSKSRTTAASLLRNCWIQASKELGI 478

Query: 536  XXXXXXTID-EERGLHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNPR 360
                    + ++  +  LI      +K  + + A    S   ++  A+Q+L+ R  D   
Sbjct: 479  DVLHDEMSEHQDLSVDQLICLNKLGNKPILAVEA----SFQASLNTANQILRGRTGDRLG 534

Query: 359  LIVVTGSLHIVSSMLADIH 303
            ++V+TGSLHIVS +LA +H
Sbjct: 535  IVVITGSLHIVSLVLATLH 553



 Score =  144 bits (364), Expect(2) = e-142
 Identities = 76/148 (51%), Positives = 99/148 (66%), Gaps = 3/148 (2%)
 Frame = -3

Query: 1881 LQHLVRRTETIA--LTSRLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGVPKGAGTES 1708
            L H  R T +I   L   +   R      +D +L D ++YM  LKNYE+ GVPK AGT+S
Sbjct: 12   LTHRKRLTSSIGVGLLRNVGSRRGFCASSDDLELKDLMEYMNSLKNYEKSGVPKDAGTDS 71

Query: 1707 DDGFDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVGCYTSPHL 1528
            +DGFD           GNPH+ F+AVH+AGTKGKGSTAAFLS+IL+  G+SVGCYTSPH+
Sbjct: 72   EDGFDLGRMRRLLELLGNPHSKFKAVHIAGTKGKGSTAAFLSSILRAEGHSVGCYTSPHI 131

Query: 1527 LTIRERISFGK-SEHVPAEMLMNIFHQV 1447
             T+RERIS G+  E V A+ L ++FH++
Sbjct: 132  QTVRERISMGRFGEPVSAKALRSLFHRI 159


>gb|KEH40416.1| tetrahydrofolylpolyglutamate synthase [Medicago truncatula]
          Length = 547

 Score =  381 bits (978), Expect(2) = e-141
 Identities = 209/382 (54%), Positives = 276/382 (72%), Gaps = 5/382 (1%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            VFTA+AF++F+ +KVDIA+IEAGLGGARDATN++ S+ LAA+VIT+VGEEHLAALGGSLE
Sbjct: 171  VFTAMAFILFADEKVDIAVIEAGLGGARDATNIISSSGLAAAVITTVGEEHLAALGGSLE 230

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            +IAMAK+GIIK G PLV+GGPF  HIE IIR+KA SM+SPVVSA + G   AVK F   D
Sbjct: 231  TIAMAKAGIIKQGCPLVLGGPFLPHIEHIIREKAVSMDSPVVSASESGNNLAVKSFSILD 290

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            GKP Q CD+ I++ +DLKL   L ++ +QM G+HQLQNA TATC ALCLR+ G+ +SD+S
Sbjct: 291  GKPCQICDIEIQIVKDLKLSCKLHDLKLQMPGAHQLQNAATATCVALCLRNLGWRISDES 350

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IR GLE+T LLGR QLL  +EA+ALG+S A IL+DGAHT+ SA+ L + IR+ +P   LA
Sbjct: 351  IRCGLERTYLLGRSQLLKSEEAKALGLSGATILLDGAHTKESAKALMNTIRMAFPKAQLA 410

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
             VVAMASDKDH  FAR++L+G   + V+LTE ++AG  +R TPAS L+D W++       
Sbjct: 411  FVVAMASDKDHAGFAREILSGAYVKTVILTEAAVAGAVTRTTPASLLRDSWIKASEVLGI 470

Query: 536  XXXXXXTID-----EERGLHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRER 372
                    +     +E+ + S  N    +     ILA     S+ D +++A+++L +R R
Sbjct: 471  DICHDGMTEYRELFKEQPVSSESNLTDGK----TILAT--ESSLKDCLRIANEIL-NRRR 523

Query: 371  DNPRLIVVTGSLHIVSSMLADI 306
            D   +IV+TGSLHIVSS+LA +
Sbjct: 524  DEKGVIVITGSLHIVSSVLASL 545



 Score =  152 bits (384), Expect(2) = e-141
 Identities = 75/142 (52%), Positives = 103/142 (72%), Gaps = 6/142 (4%)
 Frame = -3

Query: 1854 TIALTSRLSKSRLCSI-----MPEDNKLSDFLDYMEKLKNYERLGVPKGAGTESDDGFDX 1690
            T + + R    ++CS+     + ED+++ D +DY++ LKNYE+ GVP GAGT+S+DGFD 
Sbjct: 10   TSSFSHRRISRQMCSVRTLCSLREDSEMKDLVDYIDSLKNYEKSGVPTGAGTDSNDGFDL 69

Query: 1689 XXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVGCYTSPHLLTIRER 1510
                      GNPH+ F+AVH+AGTKGKGSTAAF+SNIL+  GYSVGCYTSPH+ TIRER
Sbjct: 70   GRMRRLLDRFGNPHSKFKAVHIAGTKGKGSTAAFISNILRTEGYSVGCYTSPHIQTIRER 129

Query: 1509 ISFGKS-EHVPAEMLMNIFHQV 1447
            I  G+S + V A++L N+FH++
Sbjct: 130  ILLGRSGDPVSAKLLNNLFHKI 151


>ref|XP_002513590.1| tetrahydrofolylpolyglutamate synthase, putative [Ricinus communis]
            gi|223547498|gb|EEF48993.1| tetrahydrofolylpolyglutamate
            synthase, putative [Ricinus communis]
          Length = 552

 Score =  384 bits (985), Expect(2) = e-141
 Identities = 211/383 (55%), Positives = 268/383 (69%), Gaps = 5/383 (1%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            V TA+AF +F+Q+ VDIAIIEAGLGGARDATN+L S++LA S+IT++GEEHL ALGGSLE
Sbjct: 176  VLTAIAFSLFAQENVDIAIIEAGLGGARDATNILCSSQLATSIITTIGEEHLMALGGSLE 235

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAK+GIIK GRPL++GGPF   IERI+RDKA  M +PVVSA D GIR+ +K     D
Sbjct: 236  SIAMAKAGIIKRGRPLILGGPFLPDIERILRDKASLMLAPVVSASDSGIRTTIKGLSMFD 295

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            G+  Q CD+ IRV++ L LFI+L ++ ++MLGSHQLQNA TA C ALCLR QG+ +SD S
Sbjct: 296  GRHCQLCDIKIRVKKALNLFIELSDLEMRMLGSHQLQNAATAACAALCLRDQGWKISDAS 355

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IR GLE T LLGR Q L  K+AE LGV  A IL+DGAHT+ SA+ L D +RLT+P+  LA
Sbjct: 356  IRLGLENTCLLGRSQFLASKDAEVLGVPGATILLDGAHTKESAKALVDTVRLTFPEARLA 415

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            L+VAMASDKDHLAFA + L   + E V LTE  IAGGKSR T A  L+D W++       
Sbjct: 416  LIVAMASDKDHLAFAGEFLLDKKLEAVFLTEAEIAGGKSRTTAAPLLRDYWVQACEELGI 475

Query: 536  XXXXXXTID-----EERGLHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRER 372
                    +     E   +HS  +  G      +ILA   +L I  ++++A + L+ R  
Sbjct: 476  NTLHDGMAEYRELLENEFIHSERHLGGK-----IILATNNSLEI--SLRMASEFLRGRTG 528

Query: 371  DNPRLIVVTGSLHIVSSMLADIH 303
            +   ++VVTGSLHIVSS+LA +H
Sbjct: 529  NQSSVLVVTGSLHIVSSVLARLH 551



 Score =  148 bits (374), Expect(2) = e-141
 Identities = 80/155 (51%), Positives = 104/155 (67%), Gaps = 5/155 (3%)
 Frame = -3

Query: 1896 RFISPLQHLVRRTETIALTSRLSKSR----LCSIMPEDNKLSDFLDYMEKLKNYERLGVP 1729
            RF+S     ++R   I   S    SR    L S + E  +L+DF++Y++ L N+E+ GVP
Sbjct: 2    RFVSKCPGFLQRIALIEADSGGVVSRWRKQLFSTLSEAAELNDFMNYLDSLNNFEKSGVP 61

Query: 1728 KGAGTESDDGFDXXXXXXXXXXXGNPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVG 1549
            KGAGT+SD+GFD           GNPH+ F+AVH+AGTKGKGSTAAFLSNIL+  GYSVG
Sbjct: 62   KGAGTDSDEGFDLGRMRRLMDRLGNPHSKFKAVHIAGTKGKGSTAAFLSNILRAQGYSVG 121

Query: 1548 CYTSPHLLTIRERISFGKSEHVPAEMLMNI-FHQV 1447
            CYTSPH++TIRER+S GK     + M +N  FHQ+
Sbjct: 122  CYTSPHIVTIRERMSIGKLGKPVSPMALNSHFHQI 156


>ref|XP_012440035.1| PREDICTED: dihydrofolate synthetase [Gossypium raimondii]
            gi|763785556|gb|KJB52627.1| hypothetical protein
            B456_008G270800 [Gossypium raimondii]
          Length = 550

 Score =  396 bits (1017), Expect(2) = e-141
 Identities = 208/379 (54%), Positives = 279/379 (73%)
 Frame = -1

Query: 1436 VFTALAFVIFSQQKVDIAIIEAGLGGARDATNVLRSNELAASVITSVGEEHLAALGGSLE 1257
            V TA+AF +F+Q+ VDIAIIEAGLGGARDATNV+ S+EL AS+IT++GEEHLAALGGSLE
Sbjct: 174  VLTAVAFALFAQENVDIAIIEAGLGGARDATNVISSSELDASIITTIGEEHLAALGGSLE 233

Query: 1256 SIAMAKSGIIKHGRPLVIGGPFEFHIERIIRDKAFSMNSPVVSACDPGIRSAVKFFGTED 1077
            SIAMAK+GIIKHGRP+++GGPF  HI+RI+RD+A SM SP+VSA D G+R+++K  G   
Sbjct: 234  SIAMAKAGIIKHGRPVILGGPFLPHIDRILRDRAASMFSPIVSASDAGVRTSIKGIGMFK 293

Query: 1076 GKPYQTCDMLIRVQEDLKLFIDLPNINIQMLGSHQLQNAVTATCTALCLRHQGFMLSDKS 897
            G+P Q CD++I +    +  I+L ++N+ MLG+HQLQNAVTATC ALCLR+QG+ +S+ S
Sbjct: 294  GRPSQCCDLVIELDHGSQSSIELRDLNLSMLGTHQLQNAVTATCAALCLRNQGWRISNGS 353

Query: 896  IRAGLEQTRLLGRGQLLTQKEAEALGVSEAVILVDGAHTEASARGLSDVIRLTYPDGLLA 717
            IRAGLE T L GR Q L+ KEAE LG+S + +LVDGAHT+ SA+ L + I+ T+PD  LA
Sbjct: 354  IRAGLENTFLPGRSQFLSSKEAEKLGLSGSTVLVDGAHTKDSAKALLETIQTTFPDSRLA 413

Query: 716  LVVAMASDKDHLAFARQLLTGTRPEIVLLTEVSIAGGKSRVTPASTLKDVWMRXXXXXXX 537
            +VVAMASDKDHLAFA++ L+G + E V LTE  IAGG SR T A+ L+D W++       
Sbjct: 414  IVVAMASDKDHLAFAKEFLSGKQLEAVFLTEADIAGGTSRTTSATALRDCWIQ-ASRELG 472

Query: 536  XXXXXXTIDEERGLHSLINFKGSRDKEFMILAACRNLSICDTIKVADQMLKSRERDNPRL 357
                   +   R L        +RD +   + A +N S+ D+++ A+Q+L+ R R+   +
Sbjct: 473  IEVLHDRMTRYRELFEDNFISSTRDSKHETIVAAQN-SLSDSLRFANQILRERTRNELGI 531

Query: 356  IVVTGSLHIVSSMLADIHR 300
            +VVTGSLHIVS +LA ++R
Sbjct: 532  LVVTGSLHIVSLVLASLNR 550



 Score =  135 bits (339), Expect(2) = e-141
 Identities = 68/131 (51%), Positives = 91/131 (69%), Gaps = 1/131 (0%)
 Frame = -3

Query: 1836 RLSKSRLCSIMPEDNKLSDFLDYMEKLKNYERLGVPKGAGTESDDGFDXXXXXXXXXXXG 1657
            +L   +  S   E+ +L  F  +++ LKNYE+ GVPK AGT+SDDGFD           G
Sbjct: 24   QLESKQCLSSYTEEPELEGFSQFIDSLKNYEKSGVPKDAGTDSDDGFDLGRMKRLMTRLG 83

Query: 1656 NPHTHFQAVHVAGTKGKGSTAAFLSNILQEGGYSVGCYTSPHLLTIRERISFGK-SEHVP 1480
            NP ++F++VH+AGTKGKGSTAA+LSNIL+  GYSVGCYTSPH+L+IRER+S GK  + V 
Sbjct: 84   NPLSNFKSVHIAGTKGKGSTAAYLSNILRSEGYSVGCYTSPHMLSIRERMSVGKMGKPVS 143

Query: 1479 AEMLMNIFHQV 1447
            +  L  +FH +
Sbjct: 144  SNALNCLFHSI 154