BLASTX nr result
ID: Anemarrhena21_contig00009771
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Anemarrhena21_contig00009771
(312 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_010913364.1| PREDICTED: threonine--tRNA ligase, mitochond... 197 2e-48
ref|XP_008802022.1| PREDICTED: threonine--tRNA ligase, mitochond... 196 5e-48
ref|XP_009412586.1| PREDICTED: threonine--tRNA ligase, mitochond... 195 1e-47
ref|XP_004973168.1| PREDICTED: threonine--tRNA ligase, mitochond... 195 1e-47
ref|XP_004968434.1| PREDICTED: threonine--tRNA ligase, mitochond... 194 2e-47
dbj|BAI39857.1| putative threonyl-tRNA synthetase [Oryza sativa ... 192 1e-46
gb|EEE68426.1| hypothetical protein OsJ_26793 [Oryza sativa Japo... 192 1e-46
gb|EEC83307.1| hypothetical protein OsI_28676 [Oryza sativa Indi... 192 1e-46
ref|NP_001061475.1| Os08g0295300 [Oryza sativa Japonica Group] g... 192 1e-46
ref|XP_008664856.1| PREDICTED: LOW QUALITY PROTEIN: threonine--t... 190 3e-46
ref|XP_006836673.2| PREDICTED: threonine--tRNA ligase, mitochond... 188 1e-45
ref|XP_006659275.1| PREDICTED: threonine--tRNA ligase, mitochond... 188 1e-45
gb|ERM99526.1| hypothetical protein AMTR_s00088p00069780 [Ambore... 188 1e-45
dbj|BAJ93554.1| predicted protein [Hordeum vulgare subsp. vulgare] 188 1e-45
ref|XP_002464119.1| hypothetical protein SORBIDRAFT_01g012680 [S... 188 1e-45
emb|CDM81189.1| unnamed protein product [Triticum aestivum] 188 1e-45
ref|XP_003573669.1| PREDICTED: threonine--tRNA ligase, mitochond... 187 2e-45
ref|XP_008644625.1| PREDICTED: threonine--tRNA ligase, mitochond... 187 3e-45
gb|KGN49713.1| hypothetical protein Csa_5G080790 [Cucumis sativus] 186 7e-45
ref|XP_004142299.1| PREDICTED: threonine--tRNA ligase, mitochond... 186 7e-45
>ref|XP_010913364.1| PREDICTED: threonine--tRNA ligase, mitochondrial [Elaeis
guineensis]
Length = 727
Score = 197 bits (502), Expect = 2e-48
Identities = 92/103 (89%), Positives = 101/103 (98%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFDSNEGRDTFWHSSAHILGQS+EME+GCKLCIGPCTTRGEGFYYDA+Y+DLTLNE
Sbjct: 149 LKLFKFDSNEGRDTFWHSSAHILGQSVEMEFGCKLCIGPCTTRGEGFYYDAFYDDLTLNE 208
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
E+F QIKS+A+KAVAEKQPFERIEVSR QALE+F+ENKFKVEI
Sbjct: 209 EYFDQIKSRASKAVAEKQPFERIEVSREQALEMFSENKFKVEI 251
>ref|XP_008802022.1| PREDICTED: threonine--tRNA ligase, mitochondrial [Phoenix
dactylifera]
Length = 727
Score = 196 bits (498), Expect = 5e-48
Identities = 91/103 (88%), Positives = 100/103 (97%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFDSNEGRDTFWHSSAHILGQS+EME+GCKLCIGPCTTRGEGFYYDA+Y+DLTLNE
Sbjct: 149 LKLFKFDSNEGRDTFWHSSAHILGQSVEMEFGCKLCIGPCTTRGEGFYYDAFYDDLTLNE 208
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
E+F QIKS+A+KA AEKQPFERIEVSR QALE+F+ENKFKVEI
Sbjct: 209 EYFDQIKSRASKAAAEKQPFERIEVSREQALEMFSENKFKVEI 251
>ref|XP_009412586.1| PREDICTED: threonine--tRNA ligase, mitochondrial [Musa acuminata
subsp. malaccensis]
Length = 731
Score = 195 bits (495), Expect = 1e-47
Identities = 91/103 (88%), Positives = 99/103 (96%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFD EGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDA+Y++LTLNE
Sbjct: 153 LKLFKFDDAEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAFYDELTLNE 212
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
EHF+QI+S+A+KAV EKQPFERIEVSR QALE+FAENKFKVEI
Sbjct: 213 EHFNQIQSRASKAVGEKQPFERIEVSREQALEMFAENKFKVEI 255
>ref|XP_004973168.1| PREDICTED: threonine--tRNA ligase, mitochondrial-like [Setaria
italica]
Length = 739
Score = 195 bits (495), Expect = 1e-47
Identities = 92/103 (89%), Positives = 98/103 (95%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
L+LFKFDSNEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDAYY DLTLNE
Sbjct: 161 LQLFKFDSNEGRDTFWHSSAHILGESLERAYGCKLCIGPCTTRGEGFYYDAYYNDLTLNE 220
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
EHF I+SQA+KAVAEKQPFERIEVSRA+ALE+FAENKFKVEI
Sbjct: 221 EHFGIIESQAHKAVAEKQPFERIEVSRAEALEMFAENKFKVEI 263
>ref|XP_004968434.1| PREDICTED: threonine--tRNA ligase, mitochondrial-like [Setaria
italica]
Length = 741
Score = 194 bits (493), Expect = 2e-47
Identities = 92/103 (89%), Positives = 97/103 (94%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
L+LFKFDSNEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDAYY DLTLNE
Sbjct: 163 LQLFKFDSNEGRDTFWHSSAHILGESLERVYGCKLCIGPCTTRGEGFYYDAYYNDLTLNE 222
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
EHF I+SQA KAVAEKQPFERIEVSRA+ALE+FAENKFKVEI
Sbjct: 223 EHFGIIESQAQKAVAEKQPFERIEVSRAEALEMFAENKFKVEI 265
>dbj|BAI39857.1| putative threonyl-tRNA synthetase [Oryza sativa Indica Group]
Length = 749
Score = 192 bits (487), Expect = 1e-46
Identities = 91/103 (88%), Positives = 95/103 (92%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFDSNEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDAYY DLTLNE
Sbjct: 171 LKLFKFDSNEGRDTFWHSSAHILGESLERAYGCKLCIGPCTTRGEGFYYDAYYNDLTLNE 230
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
HF I +QA KAVAEKQPFERIEVSRA+ALE+FAENKFKVEI
Sbjct: 231 THFGIIDAQAQKAVAEKQPFERIEVSRAEALEMFAENKFKVEI 273
>gb|EEE68426.1| hypothetical protein OsJ_26793 [Oryza sativa Japonica Group]
Length = 701
Score = 192 bits (487), Expect = 1e-46
Identities = 91/103 (88%), Positives = 95/103 (92%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFDSNEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDAYY DLTLNE
Sbjct: 123 LKLFKFDSNEGRDTFWHSSAHILGESLERAYGCKLCIGPCTTRGEGFYYDAYYNDLTLNE 182
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
HF I +QA KAVAEKQPFERIEVSRA+ALE+FAENKFKVEI
Sbjct: 183 THFGIIDAQAQKAVAEKQPFERIEVSRAEALEMFAENKFKVEI 225
>gb|EEC83307.1| hypothetical protein OsI_28676 [Oryza sativa Indica Group]
Length = 716
Score = 192 bits (487), Expect = 1e-46
Identities = 91/103 (88%), Positives = 95/103 (92%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFDSNEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDAYY DLTLNE
Sbjct: 167 LKLFKFDSNEGRDTFWHSSAHILGESLERAYGCKLCIGPCTTRGEGFYYDAYYNDLTLNE 226
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
HF I +QA KAVAEKQPFERIEVSRA+ALE+FAENKFKVEI
Sbjct: 227 THFGIIDAQAQKAVAEKQPFERIEVSRAEALEMFAENKFKVEI 269
>ref|NP_001061475.1| Os08g0295300 [Oryza sativa Japonica Group]
gi|38175524|dbj|BAD01219.1| putative threonyl-tRNA
synthetase [Oryza sativa Japonica Group]
gi|38175707|dbj|BAD01414.1| putative threonyl-tRNA
synthetase [Oryza sativa Japonica Group]
gi|113623444|dbj|BAF23389.1| Os08g0295300 [Oryza sativa
Japonica Group] gi|215687347|dbj|BAG91912.1| unnamed
protein product [Oryza sativa Japonica Group]
Length = 745
Score = 192 bits (487), Expect = 1e-46
Identities = 91/103 (88%), Positives = 95/103 (92%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFDSNEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDAYY DLTLNE
Sbjct: 167 LKLFKFDSNEGRDTFWHSSAHILGESLERAYGCKLCIGPCTTRGEGFYYDAYYNDLTLNE 226
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
HF I +QA KAVAEKQPFERIEVSRA+ALE+FAENKFKVEI
Sbjct: 227 THFGIIDAQAQKAVAEKQPFERIEVSRAEALEMFAENKFKVEI 269
>ref|XP_008664856.1| PREDICTED: LOW QUALITY PROTEIN: threonine--tRNA ligase,
mitochondrial-like [Zea mays]
Length = 737
Score = 190 bits (483), Expect = 3e-46
Identities = 88/103 (85%), Positives = 97/103 (94%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
L++FKFD+NEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDAYY +LTLNE
Sbjct: 159 LQMFKFDTNEGRDTFWHSSAHILGESLERAYGCKLCIGPCTTRGEGFYYDAYYNELTLNE 218
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
EHF I++QA KAVAEKQPFERIEVSRA+ALE+FAENKFKVEI
Sbjct: 219 EHFGVIENQAKKAVAEKQPFERIEVSRAEALEMFAENKFKVEI 261
>ref|XP_006836673.2| PREDICTED: threonine--tRNA ligase, mitochondrial [Amborella
trichopoda]
Length = 689
Score = 188 bits (478), Expect = 1e-45
Identities = 88/103 (85%), Positives = 94/103 (91%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKL+ FDS+EGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDA+Y DLTLNE
Sbjct: 111 LKLYTFDSHEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAFYNDLTLNE 170
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
EHF I+SQA KAV EKQPFERIEVSR QALE+F EN FK+EI
Sbjct: 171 EHFGHIQSQAKKAVDEKQPFERIEVSRQQALEMFTENGFKIEI 213
>ref|XP_006659275.1| PREDICTED: threonine--tRNA ligase, mitochondrial-like [Oryza
brachyantha]
Length = 696
Score = 188 bits (478), Expect = 1e-45
Identities = 89/103 (86%), Positives = 94/103 (91%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFDSNEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDA+Y D TLNE
Sbjct: 118 LKLFKFDSNEGRDTFWHSSAHILGESLERAYGCKLCIGPCTTRGEGFYYDAFYNDTTLNE 177
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
HF I +QA KAVAEKQPFERIEVSRA+ALE+FAENKFKVEI
Sbjct: 178 THFGIIDAQAQKAVAEKQPFERIEVSRAEALEMFAENKFKVEI 220
>gb|ERM99526.1| hypothetical protein AMTR_s00088p00069780 [Amborella trichopoda]
Length = 723
Score = 188 bits (478), Expect = 1e-45
Identities = 88/103 (85%), Positives = 94/103 (91%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKL+ FDS+EGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDA+Y DLTLNE
Sbjct: 145 LKLYTFDSHEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAFYNDLTLNE 204
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
EHF I+SQA KAV EKQPFERIEVSR QALE+F EN FK+EI
Sbjct: 205 EHFGHIQSQAKKAVDEKQPFERIEVSRQQALEMFTENGFKIEI 247
>dbj|BAJ93554.1| predicted protein [Hordeum vulgare subsp. vulgare]
Length = 711
Score = 188 bits (478), Expect = 1e-45
Identities = 89/103 (86%), Positives = 95/103 (92%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFDSNEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDA+Y+DLTLN+
Sbjct: 133 LKLFKFDSNEGRDTFWHSSAHILGESLERVYGCKLCIGPCTTRGEGFYYDAHYKDLTLND 192
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
HF I QA KAVAEKQPFERIEVSRA+ALEIFAEN+FKVEI
Sbjct: 193 THFGLIDKQAKKAVAEKQPFERIEVSRAEALEIFAENEFKVEI 235
>ref|XP_002464119.1| hypothetical protein SORBIDRAFT_01g012680 [Sorghum bicolor]
gi|241917973|gb|EER91117.1| hypothetical protein
SORBIDRAFT_01g012680 [Sorghum bicolor]
Length = 739
Score = 188 bits (478), Expect = 1e-45
Identities = 87/103 (84%), Positives = 97/103 (94%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
L++FKFD+NEGRDTFWHSSAHILG+S+E YGCKLCIGPCTTRGEGFYYDAYY+D TLNE
Sbjct: 161 LQMFKFDTNEGRDTFWHSSAHILGESIERAYGCKLCIGPCTTRGEGFYYDAYYKDQTLNE 220
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
EHF I+SQA KAVAEKQPFERIEVSRA+ALE+FAEN+FKVEI
Sbjct: 221 EHFGIIESQAKKAVAEKQPFERIEVSRAEALEMFAENEFKVEI 263
>emb|CDM81189.1| unnamed protein product [Triticum aestivum]
Length = 706
Score = 188 bits (477), Expect = 1e-45
Identities = 88/103 (85%), Positives = 95/103 (92%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFDSNEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDA+Y+DLTLN+
Sbjct: 128 LKLFKFDSNEGRDTFWHSSAHILGESLERVYGCKLCIGPCTTRGEGFYYDAHYKDLTLND 187
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
HF I QA KAVAEKQPFERIEVSRA+ALE+FAEN+FKVEI
Sbjct: 188 THFGLIDKQAKKAVAEKQPFERIEVSRAEALEVFAENEFKVEI 230
>ref|XP_003573669.1| PREDICTED: threonine--tRNA ligase, mitochondrial [Brachypodium
distachyon]
Length = 736
Score = 187 bits (476), Expect = 2e-45
Identities = 87/103 (84%), Positives = 95/103 (92%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLFKFDSNEGRDTFWHSSAHILG+SLE YGCKLCIGPCTTRGEGFYYDA+Y D+TLN+
Sbjct: 158 LKLFKFDSNEGRDTFWHSSAHILGESLERAYGCKLCIGPCTTRGEGFYYDAHYNDVTLND 217
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
HF ++ QA KAVAEKQPFERIEVSRA+ALEIFAEN+FKVEI
Sbjct: 218 THFGLVEKQAQKAVAEKQPFERIEVSRAEALEIFAENQFKVEI 260
>ref|XP_008644625.1| PREDICTED: threonine--tRNA ligase, mitochondrial-like [Zea mays]
gi|413933500|gb|AFW68051.1| hypothetical protein
ZEAMMB73_499676 [Zea mays]
Length = 739
Score = 187 bits (474), Expect = 3e-45
Identities = 86/103 (83%), Positives = 96/103 (93%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
L++FKFD+NEGRDTFWHSSAHILG+S+E YGCKLCIGPCTTRGEGFYYDAYY D TLNE
Sbjct: 161 LQMFKFDTNEGRDTFWHSSAHILGESIERAYGCKLCIGPCTTRGEGFYYDAYYNDQTLNE 220
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
EHF I++QA KAVAEKQPFERIEVSRA+ALE+FAEN+FKVEI
Sbjct: 221 EHFGIIENQAKKAVAEKQPFERIEVSRAEALEMFAENEFKVEI 263
>gb|KGN49713.1| hypothetical protein Csa_5G080790 [Cucumis sativus]
Length = 412
Score = 186 bits (471), Expect = 7e-45
Identities = 86/103 (83%), Positives = 97/103 (94%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLF FDS+EGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDA+Y+DL LN+
Sbjct: 146 LKLFTFDSDEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAFYDDLGLND 205
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
+HF QI+S A KAV+EKQPFERIEV+R QALE+F++NKFKVEI
Sbjct: 206 DHFKQIESGALKAVSEKQPFERIEVTRQQALEMFSDNKFKVEI 248
>ref|XP_004142299.1| PREDICTED: threonine--tRNA ligase, mitochondrial [Cucumis sativus]
Length = 724
Score = 186 bits (471), Expect = 7e-45
Identities = 86/103 (83%), Positives = 97/103 (94%)
Frame = -2
Query: 311 LKLFKFDSNEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAYYEDLTLNE 132
LKLF FDS+EGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDA+Y+DL LN+
Sbjct: 146 LKLFTFDSDEGRDTFWHSSAHILGQSLEMEYGCKLCIGPCTTRGEGFYYDAFYDDLGLND 205
Query: 131 EHFSQIKSQANKAVAEKQPFERIEVSRAQALEIFAENKFKVEI 3
+HF QI+S A KAV+EKQPFERIEV+R QALE+F++NKFKVEI
Sbjct: 206 DHFKQIESGALKAVSEKQPFERIEVTRQQALEMFSDNKFKVEI 248