BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Alisma22_contig00013112
         (1461 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

KMZ75435.1 Regulator of Vps4 activity in the MVB pathway protein...   323   e-104
XP_010921566.1 PREDICTED: IST1 homolog isoform X2 [Elaeis guinee...   316   e-101
XP_002282696.1 PREDICTED: IST1-like protein [Vitis vinifera] CBI...   316   e-101
XP_006842294.2 PREDICTED: vacuolar protein sorting-associated pr...   316   e-101
XP_010265126.1 PREDICTED: IST1 homolog isoform X1 [Nelumbo nucif...   316   e-101
ONK81528.1 uncharacterized protein A4U43_C01F30190 [Asparagus of...   313   e-100
XP_007044322.1 PREDICTED: IST1 homolog [Theobroma cacao] EOY0015...   312   e-100
XP_019428962.1 PREDICTED: IST1 homolog [Lupinus angustifolius]        310   2e-99
XP_011622578.1 PREDICTED: IST1 homolog isoform X1 [Amborella tri...   311   2e-99
XP_016666171.1 PREDICTED: IST1 homolog [Gossypium hirsutum] XP_0...   309   6e-99
XP_016692639.1 PREDICTED: IST1 homolog [Gossypium hirsutum]           308   2e-98
XP_011022214.1 PREDICTED: IST1 homolog [Populus euphratica]           308   2e-98
XP_017415580.1 PREDICTED: IST1 homolog [Vigna angularis] BAT9376...   307   5e-98
XP_012479516.1 PREDICTED: IST1 homolog [Gossypium raimondii] KJB...   307   5e-98
XP_008339668.1 PREDICTED: IST1 homolog isoform X2 [Malus domestica]   306   7e-98
XP_015071681.1 PREDICTED: IST1 homolog [Solanum pennellii]            306   1e-97
XP_006379742.1 hypothetical protein POPTR_0008s12030g [Populus t...   305   1e-97
XP_010551261.1 PREDICTED: IST1 homolog [Tarenaya hassleriana]         305   1e-97
XP_009394434.1 PREDICTED: IST1 homolog [Musa acuminata subsp. ma...   306   1e-97
XP_014510946.1 PREDICTED: IST1 homolog [Vigna radiata var. radiata]   305   2e-97

>KMZ75435.1 Regulator of Vps4 activity in the MVB pathway protein [Zostera
            marina]
          Length = 324

 Score =  323 bits (829), Expect = e-104
 Identities = 160/202 (79%), Positives = 183/202 (90%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS+L+SLFNRGLLG+KCKTCLNL+ISRIK LRNKRE QL  MRKEI+QFLQ+GQE IARI
Sbjct: 1    MSALNSLFNRGLLGSKCKTCLNLSISRIKLLRNKREVQLTQMRKEIFQFLQSGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHVIREQNIL+AYEIIELFCEFILARVP+LD Q++CP ELQE+IA+++FAAPRCSDV 
Sbjct: 61   RVEHVIREQNILAAYEIIELFCEFILARVPILDTQRTCPIELQESIASVIFAAPRCSDVQ 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            EL QI+NLF++KYGKEFVS+ SELRPDSGVNRVIIEKLS+K PSAEI+LA +  IAQEY 
Sbjct: 121  ELTQIKNLFTSKYGKEFVSAVSELRPDSGVNRVIIEKLSVKTPSAEIRLAVLNDIAQEYN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNG 720
             DWD S TEAEF K+HEDLL+G
Sbjct: 181  LDWDSSNTEAEFNKKHEDLLDG 202


>XP_010921566.1 PREDICTED: IST1 homolog isoform X2 [Elaeis guineensis]
          Length = 328

 Score =  316 bits (810), Expect = e-101
 Identities = 160/247 (64%), Positives = 199/247 (80%), Gaps = 2/247 (0%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+SLFNR  LG +CKTCLNLAISRIK L NKRE QL  MR+EI+Q+LQTGQE IARI
Sbjct: 1    MSVLNSLFNRATLGTRCKTCLNLAISRIKLLHNKRELQLKNMRREIFQYLQTGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHVIREQNIL+AYEIIELFCEF+LARVP+L+ Q++CP+ELQEA+A+I+FA+PRCSD+P
Sbjct: 61   RVEHVIREQNILAAYEIIELFCEFVLARVPILETQRNCPSELQEAVASIIFASPRCSDLP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            EL+Q+RNLF+AKYGKEFVS+ASELRPDS VNR IIEKLS++AP A++KL  +KAIAQEY 
Sbjct: 121  ELMQVRNLFAAKYGKEFVSAASELRPDSSVNRTIIEKLSVRAPPADLKLKVLKAIAQEYN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGID--SAISDVAEEKICLPTLASQNPNVLPQAMTKSGIK 612
             +WD S TEAEF+K+HEDLL+G +  +    V E  + +    +  P    +   K  + 
Sbjct: 181  LEWDSSNTEAEFSKKHEDLLDGSNPSNLRGPVGESSVDMLLPKNGLPGSAIKENQKDQML 240

Query: 611  SASLPAV 591
             AS+P+V
Sbjct: 241  QASIPSV 247


>XP_002282696.1 PREDICTED: IST1-like protein [Vitis vinifera] CBI27012.3 unnamed
            protein product, partial [Vitis vinifera]
          Length = 329

 Score =  316 bits (809), Expect = e-101
 Identities = 168/293 (57%), Positives = 210/293 (71%), Gaps = 10/293 (3%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+LGAKCKTCLNLAISRIK L+NKR   L  MRKEI QFLQTGQE IARI
Sbjct: 1    MSLLNQLFNRGILGAKCKTCLNLAISRIKLLQNKRSLHLKQMRKEIAQFLQTGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHVIREQNI +AYEI+ELFCEF+LARVP+L+ Q+ CPTEL+EA+A+I+FAAPRCSDVP
Sbjct: 61   RVEHVIREQNIWAAYEILELFCEFVLARVPILETQRECPTELREAVASIIFAAPRCSDVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+QI+NLF+AKYGKEF+ +ASELRPD+ VNR IIEKLS+  P+ E KL  +K IAQEY 
Sbjct: 121  DLLQIKNLFAAKYGKEFILAASELRPDTSVNRAIIEKLSVSTPTGEAKLKQLKEIAQEYN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNG-----IDSAISDVAEEKICLPTLASQNPNVLPQAMTKS 621
             +WD S TEAEF+K+HEDLL+G        AIS     +  L    +  PNV+     + 
Sbjct: 181  LEWDSSNTEAEFSKKHEDLLDGSKQVCSGGAISQAPSIQSSLQCPPTSEPNVISSRTIQQ 240

Query: 620  GIKSASLPAVTTKE-----DEYKSELKIGHLKQLSTNDDAPSLKHHLHDDVLE 477
            GI++   P   +K+     DE +  +K  +  Q+S + +  S       D+LE
Sbjct: 241  GIQALHSPNTASKKSLSSPDEVEHSIKSYNAGQVSDSKETRSQS----SDILE 289


>XP_006842294.2 PREDICTED: vacuolar protein sorting-associated protein IST1 isoform
            X2 [Amborella trichopoda]
          Length = 344

 Score =  316 bits (810), Expect = e-101
 Identities = 162/240 (67%), Positives = 196/240 (81%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+SLFNRG+LGAKCKTCL LAISRIK LRNKRE QL  MRKEI QFLQTGQEPIARI
Sbjct: 1    MSLLNSLFNRGVLGAKCKTCLKLAISRIKLLRNKREVQLKQMRKEIAQFLQTGQEPIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEH+IREQNI++AYEIIELFCEF+L RVP++D Q+ CP ELQEAIA+I+FAAPRCSD+P
Sbjct: 61   RVEHIIREQNIVAAYEIIELFCEFVLVRVPIIDTQRECPKELQEAIASIIFAAPRCSDLP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            EL+ I+NLF+AKYGKEF S+ASELRPDSGVNR IIEKLS+ AP+AE+KL  +K IA+E  
Sbjct: 121  ELMHIQNLFTAKYGKEFTSAASELRPDSGVNRGIIEKLSVTAPAAELKLKILKEIAEENN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTKSGIKSA 606
             +WD S TEAEF+K+HEDLL+G  + + + A++ I   T +SQ     P ++ +   K A
Sbjct: 181  LEWDSSSTEAEFSKKHEDLLDG-SNHVRNKAQDTIL--TFSSQKEGESPHSIVEKVQKVA 237


>XP_010265126.1 PREDICTED: IST1 homolog isoform X1 [Nelumbo nucifera]
          Length = 334

 Score =  316 bits (809), Expect = e-101
 Identities = 156/211 (73%), Positives = 184/211 (87%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRGLLGAKCKTCLNLAISRIK L+NKR+ QL  MRKEI QFLQTGQE IARI
Sbjct: 1    MSLLNQLFNRGLLGAKCKTCLNLAISRIKLLQNKRDVQLKQMRKEIAQFLQTGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHVIREQNI +AYEI+ELFCEF+LARVP+L++Q+ CP+ELQEAIA+I+FAAPRCSD+P
Sbjct: 61   RVEHVIREQNIWAAYEILELFCEFVLARVPILESQRECPSELQEAIASIIFAAPRCSDLP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            EL+QIRNLF+ KYGKEFVS+ASELRPDS VNR IIEKLSI+APSAE+KL  +K IA+EY 
Sbjct: 121  ELLQIRNLFTTKYGKEFVSAASELRPDSNVNREIIEKLSIRAPSAEVKLILLKEIAEEYD 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVA 693
             +WD S T+ EF+K+HEDLL+G +   +  A
Sbjct: 181  LEWDSSSTQIEFSKKHEDLLDGTNQLCARAA 211


>ONK81528.1 uncharacterized protein A4U43_C01F30190 [Asparagus officinalis]
          Length = 316

 Score =  313 bits (802), Expect = e-100
 Identities = 163/285 (57%), Positives = 208/285 (72%), Gaps = 2/285 (0%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+S+FNR   G +CKTC NLAISRIK LRNKREAQL  MRKEI+ +L+TGQE +ARI
Sbjct: 1    MSVLNSIFNRTTFGTRCKTCCNLAISRIKLLRNKREAQLKHMRKEIFDYLRTGQEAVARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEH+IRE+NI++AYEI+ELFCEF+LARVP+L+ QK CP ELQEAIA+I+FA+PRCSD+P
Sbjct: 61   RVEHIIREENIMAAYEILELFCEFVLARVPILETQKECPIELQEAIASIIFASPRCSDLP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            EL+QIRNLF+ KYGKEFVS+ASELRPDS VNR+IIEKL+ +APSAE+KL  +K +AQE+ 
Sbjct: 121  ELLQIRNLFTVKYGKEFVSAASELRPDSNVNRLIIEKLTARAPSAEVKLKTLKGVAQEFN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNG-IDSAISDVAEEKICLPTLASQNPNVLPQAMTKSGIKS 609
             DWD S TEAEF K+HEDLL+G + +A  +   +KI   ++   NP   P     S  + 
Sbjct: 181  LDWDSSSTEAEFTKKHEDLLDGSLPTAPQEPVIKKISDISVPKNNPAASPPVTNSSSFRG 240

Query: 608  A-SLPAVTTKEDEYKSELKIGHLKQLSTNDDAPSLKHHLHDDVLE 477
              SL      E  +K      H ++ +T     + K+    DV+E
Sbjct: 241  EDSLMNADNLEPSFKQMEITSHDERATT-----TTKNQSSSDVME 280


>XP_007044322.1 PREDICTED: IST1 homolog [Theobroma cacao] EOY00154.1 Regulator of
            Vps4 activity in the MVB pathway protein [Theobroma
            cacao]
          Length = 327

 Score =  312 bits (799), Expect = e-100
 Identities = 162/271 (59%), Positives = 202/271 (74%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+ GAKCKTCL+LAISRIK L+NKR+ QL  MRKEI QFLQ GQEPIARI
Sbjct: 1    MSLLNQLFNRGVFGAKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQAGQEPIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEH+IREQNI +AYEI+ELFCEF+LARVP+L++QK CP+EL+EA+A+I+FAAPRCSDVP
Sbjct: 61   RVEHIIREQNIWAAYEILELFCEFVLARVPILESQKECPSELREAVASIIFAAPRCSDVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+QI+NLFS KYGKEFV++ASELRPDS VNR IIEKLS+ APS EI+L  +K IAQE+ 
Sbjct: 121  DLLQIKNLFSTKYGKEFVTAASELRPDSSVNRAIIEKLSVSAPSGEIRLKVLKEIAQEHS 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTKSGIKSA 606
             +WD S TEAEF+K+HEDLL G          +++C     SQ  +++ QA  K    + 
Sbjct: 181  VEWDSSSTEAEFSKKHEDLLAG---------SKQVCAEAAVSQ--SIIRQASIKPSPSNG 229

Query: 605  SLPAVTTKEDEYKSELKIGHLKQLSTNDDAP 513
            + P + T            + KQ S +  AP
Sbjct: 230  AKPVLLT------------NAKQASQHQQAP 248


>XP_019428962.1 PREDICTED: IST1 homolog [Lupinus angustifolius]
          Length = 315

 Score =  310 bits (794), Expect = 2e-99
 Identities = 151/220 (68%), Positives = 183/220 (83%), Gaps = 5/220 (2%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+ G +CKTCLNLAISRIK L+NKR+ QL  MRKEI+QFLQ GQEPIARI
Sbjct: 1    MSLLNQLFNRGVFGTRCKTCLNLAISRIKLLQNKRDVQLKQMRKEIFQFLQAGQEPIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEH+IREQNI +AYEIIELFCEF+LARVP+++NQ+ CP+EL+EAIA+I+FAAPRCSDVP
Sbjct: 61   RVEHIIREQNIWAAYEIIELFCEFVLARVPIIENQRDCPSELREAIASIIFAAPRCSDVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+ I+NLF+ KYGKEFVS+ SELRPDSGVNR+IIEKLS+ APS E+KL  ++ IA+EY 
Sbjct: 121  DLLHIKNLFTTKYGKEFVSAVSELRPDSGVNRMIIEKLSVNAPSGEVKLKVLREIAEEYN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLN-----GIDSAISDVAEEKI 681
              WD S TEAEF K HEDLL      G+ + IS +  +KI
Sbjct: 181  LAWDSSQTEAEFRKNHEDLLGGTKPVGVGATISHIPNKKI 220


>XP_011622578.1 PREDICTED: IST1 homolog isoform X1 [Amborella trichopoda]
            XP_011622579.1 PREDICTED: IST1 homolog isoform X1
            [Amborella trichopoda]
          Length = 349

 Score =  311 bits (797), Expect = 2e-99
 Identities = 153/200 (76%), Positives = 177/200 (88%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+SLFNRG+LGAKCKTCL LAISRIK LRNKRE QL  MRKEI QFLQTGQEPIARI
Sbjct: 1    MSLLNSLFNRGVLGAKCKTCLKLAISRIKLLRNKREVQLKQMRKEIAQFLQTGQEPIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEH+IREQNI++AYEIIELFCEF+L RVP++D Q+ CP ELQEAIA+I+FAAPRCSD+P
Sbjct: 61   RVEHIIREQNIVAAYEIIELFCEFVLVRVPIIDTQRECPKELQEAIASIIFAAPRCSDLP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            EL+ I+NLF+AKYGKEF S+ASELRPDSGVNR IIEKLS+ AP+AE+KL  +K IA+E  
Sbjct: 121  ELMHIQNLFTAKYGKEFTSAASELRPDSGVNRGIIEKLSVTAPAAELKLKILKEIAEENN 180

Query: 785  FDWDPSLTEAEFAKRHEDLL 726
             +WD S TEAEF+K+HEDLL
Sbjct: 181  LEWDSSSTEAEFSKKHEDLL 200


>XP_016666171.1 PREDICTED: IST1 homolog [Gossypium hirsutum] XP_017631903.1
            PREDICTED: IST1 homolog [Gossypium arboreum] KHG01623.1
            Uncharacterized protein F383_21801 [Gossypium arboreum]
          Length = 324

 Score =  309 bits (792), Expect = 6e-99
 Identities = 165/278 (59%), Positives = 207/278 (74%), Gaps = 3/278 (1%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+ GAKCKTCL+LAISRIK L+NKR+ QL  MRKEI QFLQ GQEPIARI
Sbjct: 1    MSLLNQLFNRGVFGAKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQAGQEPIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHVIREQNI +AYEI+ELFCEF+LARVP+L++QK CP EL+EA+A+I+FAAPRCSDVP
Sbjct: 61   RVEHVIREQNIWAAYEILELFCEFVLARVPILESQKDCPPELREAVASIIFAAPRCSDVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+QI+NLF+ KYGKEFV +A+ELRPDSGVNR IIEKLS+ APS EI+L  +K IAQEY 
Sbjct: 121  DLLQIKNLFATKYGKEFVIAATELRPDSGVNRAIIEKLSVSAPSGEIRLKVLKEIAQEYS 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTKSGIKSA 606
             +WD S TEAEF K++EDLL G          +++C     SQ P+   QA  KS   + 
Sbjct: 181  VEWDSSGTEAEFNKKYEDLLAG---------SKQVCAEAAVSQAPS--KQASGKSSPSNG 229

Query: 605  S---LPAVTTKEDEYKSELKIGHLKQLSTNDDAPSLKH 501
            +   LPA      ++       H+ +L+ ++  PS+K+
Sbjct: 230  AKTILPADARPASQHHQ--VPSHVGKLTRSEIEPSVKN 265


>XP_016692639.1 PREDICTED: IST1 homolog [Gossypium hirsutum]
          Length = 324

 Score =  308 bits (789), Expect = 2e-98
 Identities = 162/276 (58%), Positives = 205/276 (74%), Gaps = 1/276 (0%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+ GAKCKTCL+LAISRIK L+NKR+ QL  MRKEI QFLQ GQEPIARI
Sbjct: 1    MSLLNQLFNRGVFGAKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQAGQEPIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHVIREQNI +AYEI+ELFCEF+LARVP+L++QK CP EL+EA+A+I+FAAPRCSDVP
Sbjct: 61   RVEHVIREQNIWAAYEILELFCEFVLARVPILESQKDCPPELREAVASIIFAAPRCSDVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+QI+NLF+ KYGKEFV +A+ELRPDSGVNR IIEKLS+ A S EI+L  +K IAQEY 
Sbjct: 121  DLLQIKNLFATKYGKEFVIAATELRPDSGVNRAIIEKLSVSAASGEIRLKVLKEIAQEYS 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTKSGIKSA 606
             +WD S TEAEF K++EDLL G          +++C     SQ PN   QA  KS   + 
Sbjct: 181  VEWDSSGTEAEFNKKYEDLLAG---------SKQVCAEAAVSQAPN--KQASGKSSPSNG 229

Query: 605  SLPAVTTKEDEYKSELKI-GHLKQLSTNDDAPSLKH 501
            +   + T         ++  H+ +L++++   S+K+
Sbjct: 230  AKTILPTDARPASQHHQVPSHMSKLTSSEIESSVKN 265


>XP_011022214.1 PREDICTED: IST1 homolog [Populus euphratica]
          Length = 314

 Score =  308 bits (788), Expect = 2e-98
 Identities = 162/289 (56%), Positives = 210/289 (72%), Gaps = 10/289 (3%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+ G+KCKTCL+LAISRIK L+NKR+ QL  MRKEI QFLQ GQE IARI
Sbjct: 1    MSLLNQLFNRGVFGSKCKTCLSLAISRIKLLQNKRDLQLKHMRKEIAQFLQAGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHVIREQNI +AYEI+ELFCEF+L RVP+L++QK CP EL+EAIA+I+FAAPRCS+VP
Sbjct: 61   RVEHVIREQNIRAAYEILELFCEFVLVRVPILESQKECPAELREAIASIIFAAPRCSEVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+QI+NLF+AKYGKEF  +ASELRPDSGVNR IIE+LS++AP AE +L  +K IAQE+ 
Sbjct: 121  DLLQIKNLFAAKYGKEFNMAASELRPDSGVNRAIIERLSVRAPPAEARLKVLKEIAQEFS 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTKSGIKSA 606
             +WD S TEAE  K+HEDLL G    ++D                 +LPQA TK    S 
Sbjct: 181  LEWDSSNTEAELGKKHEDLLGGSKQIMADA----------------ILPQAPTKQS-SSL 223

Query: 605  SLPA------VTTKEDEYKSELK----IGHLKQLSTNDDAPSLKHHLHD 489
            S P+      +TT   +    ++    +G++ Q++ N+  PS+++++ D
Sbjct: 224  SPPSNGAHSTLTTDNKQGSHHIEAPALVGNMPQVNANEIEPSIRNYMAD 272


>XP_017415580.1 PREDICTED: IST1 homolog [Vigna angularis] BAT93768.1 hypothetical
            protein VIGAN_08029800 [Vigna angularis var. angularis]
          Length = 324

 Score =  307 bits (786), Expect = 5e-98
 Identities = 166/283 (58%), Positives = 203/283 (71%), Gaps = 11/283 (3%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+LG +CKTCLNLAISRIK L+NKR+ QL  M KEI QFLQ GQE IARI
Sbjct: 1    MSLLNQLFNRGVLGTRCKTCLNLAISRIKLLQNKRDVQLKQMCKEISQFLQAGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEH+IREQNI +AYEI+ELFCEF+LARVP+++NQ+ CP+EL+EAIA+I+FAAPRCSDVP
Sbjct: 61   RVEHIIREQNIWAAYEILELFCEFVLARVPIIENQRECPSELREAIASIIFAAPRCSDVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+ I+NLF+ KYGKEFVS+ASELRPDSGVNR IIEKLS+ APS E+KL  ++ IA+EY 
Sbjct: 121  DLLHIKNLFTTKYGKEFVSAASELRPDSGVNRTIIEKLSVTAPSGEVKLKVLREIAEEYN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTKSGIKSA 606
              WD S TEAEF K HEDLL G     + V      L    S+N +  P + T    K  
Sbjct: 181  IAWDSSKTEAEFRKNHEDLLGGAKQVSAGVT-----LSHTPSRNGSNNPPSRT----KEP 231

Query: 605  SLPAVTTKEDEYK-----------SELKIGHLKQLSTNDDAPS 510
            S  +V  K+ EYK           S L I  ++Q   N+D P+
Sbjct: 232  SNKSVHDKQ-EYKQLEAPSLSNNNSALNINEIEQSHKNNDVPA 273


>XP_012479516.1 PREDICTED: IST1 homolog [Gossypium raimondii] KJB31432.1 hypothetical
            protein B456_005G191700 [Gossypium raimondii]
          Length = 324

 Score =  307 bits (786), Expect = 5e-98
 Identities = 162/276 (58%), Positives = 205/276 (74%), Gaps = 1/276 (0%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+ GAKCKTCL+LAISRIK L+NKR+ QL  MRKEI QFLQ GQEPIARI
Sbjct: 1    MSLLNQLFNRGVFGAKCKTCLSLAISRIKLLQNKRDLQLKNMRKEIAQFLQAGQEPIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHVIREQNI +AYEI+ELFCEF+LARVP+L++QK CP EL+EA+A+I+FAAPRCSDVP
Sbjct: 61   RVEHVIREQNIWAAYEILELFCEFVLARVPILESQKDCPPELREAVASIIFAAPRCSDVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+QI+NLF+ KYGKEFV +A ELRPDSGVNR IIEKLS+ APS EI+L  +K IAQEY 
Sbjct: 121  DLLQIKNLFATKYGKEFVIAAMELRPDSGVNRAIIEKLSVSAPSGEIRLKVLKEIAQEYS 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTKSGIKSA 606
             +WD S TEAEF K++EDLL G          +++C     SQ P+   QA  KS   + 
Sbjct: 181  VEWDSSGTEAEFNKKYEDLLAG---------SKQVCAEAAVSQAPS--KQASGKSLPSNG 229

Query: 605  SLPAVTTKEDEYKSELKI-GHLKQLSTNDDAPSLKH 501
            +   + T         ++  H+ +L++++   S+K+
Sbjct: 230  AKTILPTDARPASQHHQVPSHMSKLTSSEIESSVKN 265


>XP_008339668.1 PREDICTED: IST1 homolog isoform X2 [Malus domestica]
          Length = 315

 Score =  306 bits (784), Expect = 7e-98
 Identities = 162/274 (59%), Positives = 197/274 (71%), Gaps = 5/274 (1%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+ G KCKTCLNL ISRIK L+NKR+ QL  MRKEI QFLQ GQE IARI
Sbjct: 1    MSLLNQLFNRGVFGTKCKTCLNLGISRIKLLQNKRDVQLKHMRKEIAQFLQAGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHVIREQNI +AYEI+ELFCEF+LARVP+++NQ  CPTEL+EAI++I+FAAPRCSDVP
Sbjct: 61   RVEHVIREQNIWAAYEILELFCEFVLARVPIIENQXECPTELREAISSIIFAAPRCSDVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+QI NLF+AKYGKEFVS+ASELRPDSGVNR IIEKLS+ APS E +L  +K IAQEY 
Sbjct: 121  DLMQITNLFTAKYGKEFVSAASELRPDSGVNRTIIEKLSVSAPSGEARLKVLKEIAQEYK 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGID-----SAISDVAEEKICLPTLASQNPNVLPQAMTKS 621
             +WD S TEAEF+K+H+DLL G       +A S    +++   +  S   + +  A  K 
Sbjct: 181  LNWDSSDTEAEFSKKHDDLLGGXKHVSGAAAPSQAPAKRVTFSSPPSNGAHTIVPADVKQ 240

Query: 620  GIKSASLPAVTTKEDEYKSELKIGHLKQLSTNDD 519
              +    P        + SE+      Q S NDD
Sbjct: 241  EPQYLXAPRSLINTPSFSSEI------QPSGNDD 268


>XP_015071681.1 PREDICTED: IST1 homolog [Solanum pennellii]
          Length = 323

 Score =  306 bits (783), Expect = 1e-97
 Identities = 167/297 (56%), Positives = 207/297 (69%), Gaps = 14/297 (4%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRGL G+KCKTCL LAISRIK L+NKR+AQL  MRKEI QFLQTGQE IARI
Sbjct: 1    MSMLNQLFNRGLQGSKCKTCLTLAISRIKLLQNKRDAQLRLMRKEIAQFLQTGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEH+IREQN+ +AYEI+ELFCEF+ ARVP+L++QK CP+EL+EA+A+I+FAAPRCSD+P
Sbjct: 61   RVEHIIREQNVWAAYEILELFCEFVYARVPILESQKECPSELREAVASIIFAAPRCSDLP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+ +RNLF+AKYGKEF+++ASELRPD+ VNR I+EKLS+ APSAE+KL  +K IA+EY 
Sbjct: 121  DLLHVRNLFAAKYGKEFIAAASELRPDTSVNRTIVEKLSVGAPSAELKLNVLKEIAKEYN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTKS----- 621
             +WD S TEAE +K+ EDLLNG          ++I  P  AS  PN      T       
Sbjct: 181  VEWDSSYTEAELSKKPEDLLNG---------PKQIAAPVRASLEPNTRGHPPTSENPVTS 231

Query: 620  -----GIKSASLPAVTTKEDEYKSEL-KIGHLKQLSTNDDAPSLKHHLH---DDVLE 477
                 G KS   P   TK   + + + K  H    ST  DA  +K        DVLE
Sbjct: 232  PNGNRGAKSLDSPTSVTKAPLWSANIDKPSHSD--STLADAVDIKKDTRPETSDVLE 286


>XP_006379742.1 hypothetical protein POPTR_0008s12030g [Populus trichocarpa]
            ABK94695.1 unknown [Populus trichocarpa] ERP57539.1
            hypothetical protein POPTR_0008s12030g [Populus
            trichocarpa]
          Length = 314

 Score =  305 bits (782), Expect = 1e-97
 Identities = 162/288 (56%), Positives = 210/288 (72%), Gaps = 9/288 (3%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+ G+KCKTCLNLAISRIK L+NKR+ QL  MRKEI QFLQ GQE IARI
Sbjct: 1    MSLLNQLFNRGVFGSKCKTCLNLAISRIKLLQNKRDLQLKHMRKEIAQFLQAGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHVIREQNI +AYEI+ELFCEF+L RVP+L++QK CP EL+EAIA+I+FAAPRCS+VP
Sbjct: 61   RVEHVIREQNIRAAYEILELFCEFVLVRVPILESQKECPAELREAIASIIFAAPRCSEVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+QI+NLF+AKYGKEF  +ASELRPDSGVNR IIE+LS++AP AE +L  +K IAQE+ 
Sbjct: 121  DLLQIKNLFAAKYGKEFNMAASELRPDSGVNRAIIERLSVRAPPAEARLKVLKEIAQEFS 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTK--SGIK 612
             +WD S TEAE  K+HEDLL G    ++D                 +LPQA TK  S + 
Sbjct: 181  LEWDSSNTEAELGKKHEDLLGGSKEIMADA----------------ILPQAPTKQNSPLS 224

Query: 611  SASLPAVTT-----KEDEYKSELK--IGHLKQLSTNDDAPSLKHHLHD 489
              S  A +T     K+  ++ E    + ++ +++ N+  PS+++++ D
Sbjct: 225  PPSNGAHSTLNTDNKQGSHRLEAPALVSNMPRVNANEIEPSIRNYMAD 272


>XP_010551261.1 PREDICTED: IST1 homolog [Tarenaya hassleriana]
          Length = 315

 Score =  305 bits (782), Expect = 1e-97
 Identities = 163/272 (59%), Positives = 196/272 (72%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+ GAKCKTCLNLAISRIK L+NKR+ QL  M+KEI QFLQ GQEPIARI
Sbjct: 1    MSLLNQLFNRGIFGAKCKTCLNLAISRIKLLQNKRDMQLKHMKKEIAQFLQAGQEPIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEHV+RE N+ +AYEI+ELFCEF+LARVPVL+ QK CP EL+EAI++I+FAAPRCS+VP
Sbjct: 61   RVEHVVRELNLWAAYEILELFCEFVLARVPVLETQKECPRELREAISSIIFAAPRCSEVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+QIRNLF +KYGKEF+ SASELRPDSGVNR IIEKLS  APS E+KL  +K IAQEY 
Sbjct: 121  DLLQIRNLFGSKYGKEFIMSASELRPDSGVNRTIIEKLSPSAPSGEVKLKLLKEIAQEYN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTKSGIKSA 606
             +WD S TEAEF K HEDLL G          ++I      +++P        K+  KS+
Sbjct: 181  LNWDSSATEAEFRKSHEDLLGG---------SKEISRKAAVTESP-----LSKKNSFKSS 226

Query: 605  SLPAVTTKEDEYKSELKIGHLKQLSTNDDAPS 510
            +  A T+ E E         L+QL     A S
Sbjct: 227  T--ATTSSESEILPAETEQRLRQLQAQTPAAS 256


>XP_009394434.1 PREDICTED: IST1 homolog [Musa acuminata subsp. malaccensis]
          Length = 326

 Score =  306 bits (783), Expect = 1e-97
 Identities = 168/293 (57%), Positives = 208/293 (70%), Gaps = 10/293 (3%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS+L+SLFNR   G +CKTCLNLAISRIK LRNKRE QL  MRKEI Q++QTGQE IARI
Sbjct: 1    MSALNSLFNRAPFGTRCKTCLNLAISRIKLLRNKRELQLKQMRKEISQYIQTGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVE++IREQNIL+AYEIIELFCEF+LARVPVL++Q+ CP ELQEAI++I+FA+PRCSD+P
Sbjct: 61   RVEYIIREQNILAAYEIIELFCEFVLARVPVLESQRDCPLELQEAISSIIFASPRCSDLP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            EL+ IRNLFS KYGKEFV++ASELRP+S VNR+IIEKL+ +AP AE+K+  +KAIAQEY 
Sbjct: 121  ELLHIRNLFSTKYGKEFVAAASELRPESNVNRMIIEKLTARAPPAELKIKVLKAIAQEYN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDS--------AISDVAEEKICLPT--LASQNPNVLPQ 636
              WD S TEAE +K HEDLL+G++         A S    +K  LP   +       LPQ
Sbjct: 181  LVWDSSSTEAELSKNHEDLLDGLNPTNSQATVIASSTGTSQKDGLPVSPVRENRTEQLPQ 240

Query: 635  AMTKSGIKSASLPAVTTKEDEYKSELKIGHLKQLSTNDDAPSLKHHLHDDVLE 477
                S    +SL +  +  D+ K++      + LS   D  S       DVLE
Sbjct: 241  TSIPSVNNLSSLASKISVSDDGKADAVRSETEVLSI--DKTSCSSQSSSDVLE 291


>XP_014510946.1 PREDICTED: IST1 homolog [Vigna radiata var. radiata]
          Length = 324

 Score =  305 bits (782), Expect = 2e-97
 Identities = 158/276 (57%), Positives = 198/276 (71%), Gaps = 5/276 (1%)
 Frame = -2

Query: 1325 MSSLSSLFNRGLLGAKCKTCLNLAISRIKHLRNKREAQLACMRKEIYQFLQTGQEPIARI 1146
            MS L+ LFNRG+LG +CKTCLNLAISRIK L+NKR+ QL  M KEI QFLQ GQE IARI
Sbjct: 1    MSLLNQLFNRGVLGTRCKTCLNLAISRIKLLQNKRDVQLKQMCKEISQFLQAGQEAIARI 60

Query: 1145 RVEHVIREQNILSAYEIIELFCEFILARVPVLDNQKSCPTELQEAIATIVFAAPRCSDVP 966
            RVEH+IREQNI +AYEI+ELFCEF+LARVP+++NQ+ CP+EL+EAIA+I+FAAPRCSDVP
Sbjct: 61   RVEHIIREQNIWAAYEILELFCEFVLARVPIIENQRECPSELREAIASIIFAAPRCSDVP 120

Query: 965  ELIQIRNLFSAKYGKEFVSSASELRPDSGVNRVIIEKLSIKAPSAEIKLAFMKAIAQEYG 786
            +L+ ++NLF+ KYGKEFVS+ASELRPDSGVNR IIEKLS+ APS E+KL  ++ IA+EY 
Sbjct: 121  DLLHVKNLFTTKYGKEFVSAASELRPDSGVNRTIIEKLSVTAPSGEVKLKVLREIAEEYN 180

Query: 785  FDWDPSLTEAEFAKRHEDLLNGIDSAISDVAEEKICLPTLASQNPNVLPQAMTKS----- 621
              WD S TEAEF K HEDLL G     + V          ++ +P+   +   KS     
Sbjct: 181  IAWDSSKTEAEFRKNHEDLLGGAKQLSAGVTLSHTPSRNGSNNSPSRTTEPSNKSVHDKQ 240

Query: 620  GIKSASLPAVTTKEDEYKSELKIGHLKQLSTNDDAP 513
              K    P+++       S L I  ++Q   N+D P
Sbjct: 241  EYKQLEAPSLSNN----NSALNINEIEQSHKNNDVP 272