BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Alisma22_contig00001693
         (2321 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

JAT46693.1 tRNA wybutosine-synthesizing protein 1 [Anthurium amn...   119   9e-26
XP_018821629.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   120   2e-24
JAT67814.1 tRNA wybutosine-synthesizing protein 1 [Anthurium amn...   119   3e-24
XP_008783493.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   119   3e-24
XP_008783492.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   119   3e-24
XP_008783491.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   119   3e-24
XP_009399072.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   118   5e-24
ONK64920.1 uncharacterized protein A4U43_C07F31480 [Asparagus of...   119   6e-24
ONI32680.1 hypothetical protein PRUPE_1G379500 [Prunus persica] ...   117   1e-23
XP_010263670.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   117   1e-23
XP_008220799.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   116   3e-23
XP_016193063.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   109   6e-23
KRH15213.1 hypothetical protein GLYMA_14G075000 [Glycine max]         114   8e-23
XP_002281468.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   114   9e-23
XP_008377398.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   114   1e-22
XP_009365568.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   114   2e-22
XP_003544413.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   114   2e-22
OAY80972.1 S-adenosyl-L-methionine-dependent tRNA 4-demethylwyos...   114   2e-22
XP_015884574.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   112   2e-22
XP_009336348.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA...   112   4e-22

>JAT46693.1 tRNA wybutosine-synthesizing protein 1 [Anthurium amnicola]
          Length = 341

 Score =  119 bits (299), Expect = 9e-26
 Identities = 76/203 (37%), Positives = 113/203 (55%), Gaps = 3/203 (1%)
 Frame = -3

Query: 2244 QGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIASLRKDE 2071
            +GKL   SETG    L+ RL   LS   +  DLVD   Y P+DL KE+++L++AS  +D 
Sbjct: 54   RGKLFFASETGTSEALARRLLRLLSSEGLAFDLVDPSGYEPEDLQKENVVLMVASTWEDG 113

Query: 2070 RISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSICMRSL 1894
            R     + +A+WLS+ A D     ++ A C+F VFGVG ++  + +N   R FS  MR+L
Sbjct: 114  RPPPNAEFLARWLSESAEDFRVGSILLARCKFAVFGVGSRSYGEGFNAAARGFSRWMRAL 173

Query: 1893 GASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSDVCNEEMPYICVEGLKFDEYN 1714
            GASEILP+ E D ++  +DEVF  WSTK+V  LK ++      +  P + +E    +   
Sbjct: 174  GASEILPVGEGDVDAGDVDEVFGSWSTKVVRLLKGDLHE---KDRRPGLNLENGTLEGSE 230

Query: 1713 EDGDHLYETAASTFRTATTENED 1645
            E+ D  Y+       +A  + ED
Sbjct: 231  EEED--YDEDEEELESAMADMED 251


>XP_018821629.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Juglans regia]
          Length = 648

 Score =  120 bits (300), Expect = 2e-24
 Identities = 69/165 (41%), Positives = 99/165 (60%), Gaps = 3/165 (1%)
 Frame = -3

Query: 2268 TRVSSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILI 2095
            T  + S S+ K+   S+TG    L+ RL   LS + ++ DLVD ++Y P+DLPKESL+LI
Sbjct: 43   TNPNLSHSKAKIFFVSQTGTSEALARRLFDLLSSNSLSFDLVDPKDYEPEDLPKESLVLI 102

Query: 2094 IASLRKDERISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRH 1918
            IAS  +D +     +  + WLS+ A D     L+ + C+F VFGVG  A    +N + R 
Sbjct: 103  IASTWEDGKPPPNAKFFSSWLSESAEDFRVGSLLLSRCKFAVFGVGSGAYGATFNAVARD 162

Query: 1917 FSICMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANI 1783
            +S  MR+LGA+EILP+ E D ++   DEVF  WS K+V  LK  +
Sbjct: 163  YSKQMRALGANEILPVWEGDVDNGDFDEVFEAWSAKVVGLLKVGV 207


>JAT67814.1 tRNA wybutosine-synthesizing protein 1 [Anthurium amnicola]
          Length = 665

 Score =  119 bits (299), Expect = 3e-24
 Identities = 76/203 (37%), Positives = 113/203 (55%), Gaps = 3/203 (1%)
 Frame = -3

Query: 2244 QGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIASLRKDE 2071
            +GKL   SETG    L+ RL   LS   +  DLVD   Y P+DL KE+++L++AS  +D 
Sbjct: 54   RGKLFFASETGTSEALARRLLRLLSSEGLAFDLVDPSGYEPEDLQKENVVLMVASTWEDG 113

Query: 2070 RISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSICMRSL 1894
            R     + +A+WLS+ A D     ++ A C+F VFGVG ++  + +N   R FS  MR+L
Sbjct: 114  RPPPNAEFLARWLSESAEDFRVGSILLARCKFAVFGVGSRSYGEGFNAAARGFSRWMRAL 173

Query: 1893 GASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSDVCNEEMPYICVEGLKFDEYN 1714
            GASEILP+ E D ++  +DEVF  WSTK+V  LK ++      +  P + +E    +   
Sbjct: 174  GASEILPVGEGDVDAGDVDEVFGSWSTKVVRLLKGDLHE---KDRRPGLNLENGTLEGSE 230

Query: 1713 EDGDHLYETAASTFRTATTENED 1645
            E+ D  Y+       +A  + ED
Sbjct: 231  EEED--YDEDEEELESAMADMED 251


>XP_008783493.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like isoform X3 [Phoenix dactylifera]
          Length = 615

 Score =  119 bits (298), Expect = 3e-24
 Identities = 68/177 (38%), Positives = 104/177 (58%), Gaps = 3/177 (1%)
 Frame = -3

Query: 2295 RLGMETPHITRVSSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDD 2122
            RL  + P+      S  +GKL   S+TG  R L+ RL   L  + +  DLVD   Y P+D
Sbjct: 38   RLSSQNPN----RDSSPRGKLFFASQTGTSRALAGRLADRLRAAALPFDLVDPRGYEPED 93

Query: 2121 LPKESLILIIASLRKDERISRPTQVVAQWLSQRAADSNQQKLM-KACRFCVFGVGPQARR 1945
            LPKE+L+LI+AS  +D +     + +A+WL++ A D     L+   CRF VFGVG ++  
Sbjct: 94   LPKETLVLIVASTWEDGKPPPNAEFLARWLAESAEDFRVGSLLLSGCRFAVFGVGSRSYG 153

Query: 1944 KWYNNLGRHFSICMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSD 1774
            + +N   + FS  MR++GA+E+LP+ E D ++  +DEVF  WS K++  LK ++  D
Sbjct: 154  ESFNATAKDFSRWMRAVGAAEMLPVWEGDVDNGDMDEVFDSWSQKVIGLLKGDVCED 210


>XP_008783492.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like isoform X1 [Phoenix dactylifera]
          Length = 648

 Score =  119 bits (298), Expect = 3e-24
 Identities = 68/177 (38%), Positives = 104/177 (58%), Gaps = 3/177 (1%)
 Frame = -3

Query: 2295 RLGMETPHITRVSSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDD 2122
            RL  + P+      S  +GKL   S+TG  R L+ RL   L  + +  DLVD   Y P+D
Sbjct: 38   RLSSQNPN----RDSSPRGKLFFASQTGTSRALAGRLADRLRAAALPFDLVDPRGYEPED 93

Query: 2121 LPKESLILIIASLRKDERISRPTQVVAQWLSQRAADSNQQKLM-KACRFCVFGVGPQARR 1945
            LPKE+L+LI+AS  +D +     + +A+WL++ A D     L+   CRF VFGVG ++  
Sbjct: 94   LPKETLVLIVASTWEDGKPPPNAEFLARWLAESAEDFRVGSLLLSGCRFAVFGVGSRSYG 153

Query: 1944 KWYNNLGRHFSICMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSD 1774
            + +N   + FS  MR++GA+E+LP+ E D ++  +DEVF  WS K++  LK ++  D
Sbjct: 154  ESFNATAKDFSRWMRAVGAAEMLPVWEGDVDNGDMDEVFDSWSQKVIGLLKGDVCED 210


>XP_008783491.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like isoform X2 [Phoenix dactylifera]
          Length = 648

 Score =  119 bits (298), Expect = 3e-24
 Identities = 68/177 (38%), Positives = 104/177 (58%), Gaps = 3/177 (1%)
 Frame = -3

Query: 2295 RLGMETPHITRVSSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDD 2122
            RL  + P+      S  +GKL   S+TG  R L+ RL   L  + +  DLVD   Y P+D
Sbjct: 38   RLSSQNPN----RDSSPRGKLFFASQTGTSRALAGRLADRLRAAALPFDLVDPRGYEPED 93

Query: 2121 LPKESLILIIASLRKDERISRPTQVVAQWLSQRAADSNQQKLM-KACRFCVFGVGPQARR 1945
            LPKE+L+LI+AS  +D +     + +A+WL++ A D     L+   CRF VFGVG ++  
Sbjct: 94   LPKETLVLIVASTWEDGKPPPNAEFLARWLAESAEDFRVGSLLLSGCRFAVFGVGSRSYG 153

Query: 1944 KWYNNLGRHFSICMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSD 1774
            + +N   + FS  MR++GA+E+LP+ E D ++  +DEVF  WS K++  LK ++  D
Sbjct: 154  ESFNATAKDFSRWMRAVGAAEMLPVWEGDVDNGDMDEVFDSWSQKVIGLLKGDVCED 210


>XP_009399072.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Musa acuminata subsp. malaccensis]
          Length = 647

 Score =  118 bits (296), Expect = 5e-24
 Identities = 77/185 (41%), Positives = 108/185 (58%), Gaps = 6/185 (3%)
 Frame = -3

Query: 2244 QGKLLITSETGRVLSHRLQGTLSDSL----INVDLVDTEEYCPDDLPKESLILIIASLRK 2077
            +GK++  S TG   S  L G LSD L    +  DLVD   Y P+DLPKE+L+LI+AS  +
Sbjct: 54   RGKIIYASATGT--SRSLAGRLSDCLRSHGLAFDLVDPRHYEPEDLPKEALVLIVASTWE 111

Query: 2076 DERISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSICMR 1900
            + R     Q +A+WL++ AAD     L+ A CRF VFGVG ++    +N   + FS  MR
Sbjct: 112  EGRPPADAQFLARWLAESAADFRVGSLLLAKCRFAVFGVGSKSYGDSFNAAAKDFSKWMR 171

Query: 1899 SLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSDVCNEE-MPYICVEGLKFD 1723
            +LGASE+ P+ E D +S  +DEVF  WS ++V+ LK     D+  E       VE + FD
Sbjct: 172  ALGASEMAPVWEGDVDSGDVDEVFEVWSRRIVALLKG---EDLKKESGHSGSMVESVGFD 228

Query: 1722 EYNED 1708
            E +E+
Sbjct: 229  ESDEE 233


>ONK64920.1 uncharacterized protein A4U43_C07F31480 [Asparagus officinalis]
          Length = 749

 Score =  119 bits (297), Expect = 6e-24
 Identities = 77/229 (33%), Positives = 121/229 (52%), Gaps = 5/229 (2%)
 Frame = -3

Query: 2244 QGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIASLRKDE 2071
            +GKL   S+TG  + L+ RL   L  + I  DLVD + Y P+DLPKE+L+L +AS  +D 
Sbjct: 53   RGKLFFASQTGTSKTLAARLLDLLQSNSIPFDLVDPKTYEPEDLPKETLVLFVASTWEDG 112

Query: 2070 RISRPTQVVAQWLSQRAADSNQQKL-MKACRFCVFGVGPQARRKWYNNLGRHFSICMRSL 1894
            +     Q +AQWL++ A D     L +  C+F VFGVG +A  + +N +G+  S+ MR+L
Sbjct: 113  KPPSNAQFLAQWLAESAEDFRVGSLILSRCKFAVFGVGSRAYDERFNKVGKDLSLWMRAL 172

Query: 1893 GASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSDVCNEEMPYICVEGL--KFDE 1720
            GA+E++P+ E D +   LD  F  WS ++V  LK     D    ++  +  E +  + D+
Sbjct: 173  GAAELVPVAEGDVDGGDLDVRFGLWSERVVKVLKGEGGDDGVESDVFDVLEEEVEDEDDD 232

Query: 1719 YNEDGDHLYETAASTFRTATTENEDKIMAHGANVYVLLEHTDDNPSEKS 1573
            Y+++ D   E+ A        +   K    G      L + D+   EKS
Sbjct: 233  YDDEEDEEVESEAVDMEDIAGKAPSKKSGSG------LANGDNGAREKS 275


>ONI32680.1 hypothetical protein PRUPE_1G379500 [Prunus persica] ONI32681.1
            hypothetical protein PRUPE_1G379500 [Prunus persica]
          Length = 643

 Score =  117 bits (293), Expect = 1e-23
 Identities = 74/189 (39%), Positives = 107/189 (56%), Gaps = 5/189 (2%)
 Frame = -3

Query: 2259 SSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIAS 2086
            SSS  +GKL   S+TG  + L+HRL   L+ + +  DLVD   Y P+DLPKESL+LIIAS
Sbjct: 42   SSSPRKGKLFFISQTGTSKALAHRLLDLLTSNGLAFDLVDPNHYEPEDLPKESLVLIIAS 101

Query: 2085 LRKDERISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSI 1909
               D +     +  + WL++ A D     L+ + C+F VFGVG +A    +N +G+  + 
Sbjct: 102  TWDDGKPPANAKFFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGATFNAVGKDVAK 161

Query: 1908 CMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSD--VCNEEMPYICVEG 1735
             MR+LGASEI  + E D +   +DEVF  WS K++  LK  +  +  V +  +   C E 
Sbjct: 162  RMRALGASEIFSIWEGDVDGGDIDEVFEAWSGKVLRFLKGGVAENGGVLSNGVGAEC-EA 220

Query: 1734 LKFDEYNED 1708
              FD  +ED
Sbjct: 221  ESFDGSDED 229


>XP_010263670.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Nelumbo nucifera]
          Length = 652

 Score =  117 bits (293), Expect = 1e-23
 Identities = 66/157 (42%), Positives = 98/157 (62%), Gaps = 3/157 (1%)
 Frame = -3

Query: 2244 QGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIASLRKDE 2071
            +GKL   S+TG  + L+HRL   L+ + I  DLVD ++Y P+DL KE+L+LI+AS  +D 
Sbjct: 57   RGKLFFVSQTGTSKTLAHRLFDILNSNSIPFDLVDPKDYEPEDLSKETLVLIVASTWEDG 116

Query: 2070 RISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSICMRSL 1894
            +    +  +A WL++   D     L+ + C+F VFGVG Q+  + +N + + FS  MR+L
Sbjct: 117  KPPPNSNFLANWLAESVDDFRVGSLLFSDCKFAVFGVGSQSYGETFNAVAKDFSKRMRAL 176

Query: 1893 GASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANI 1783
            GASE+LP+ E D ++  LD VF  WS KLV  LK  +
Sbjct: 177  GASEVLPVCEGDVDAGDLDAVFGAWSWKLVRVLKGGL 213


>XP_008220799.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Prunus mume]
          Length = 643

 Score =  116 bits (290), Expect = 3e-23
 Identities = 72/191 (37%), Positives = 108/191 (56%), Gaps = 5/191 (2%)
 Frame = -3

Query: 2259 SSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIAS 2086
            SSS  +GKL   S+TG  + L+HRL   L+ + +  DLVD   Y P+DLPKE+L+LIIAS
Sbjct: 42   SSSPRKGKLFFISQTGTSKALAHRLLDLLTSNGLGFDLVDPNHYEPEDLPKETLVLIIAS 101

Query: 2085 LRKDERISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSI 1909
               D +     +  + WL++ A D     L+ + C+F VFGVG +A    +N +G+  + 
Sbjct: 102  TWDDGKPPANAKFFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGATFNAVGKDVAK 161

Query: 1908 CMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSD--VCNEEMPYICVEG 1735
             MR+LGA+EI  + E D +   +DEVF  WS K++  LK  +  +  V +  +   C E 
Sbjct: 162  RMRALGAAEIFSIWEGDVDGGDIDEVFEAWSGKVLRFLKGGVAENGGVLSNGVGAEC-EA 220

Query: 1734 LKFDEYNEDGD 1702
              FD  +ED +
Sbjct: 221  ESFDGSDEDDE 231


>XP_016193063.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Arachis ipaensis]
          Length = 263

 Score =  109 bits (272), Expect = 6e-23
 Identities = 64/172 (37%), Positives = 97/172 (56%), Gaps = 3/172 (1%)
 Frame = -3

Query: 2271 ITRVSSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLIL 2098
            +T  S   ++GK+L  S+TG  + L+ RL   L+ + +  DLVD + Y P+DLPKE+L+L
Sbjct: 38   LTLSSKHTTRGKILFVSQTGTSKNLAQRLHNLLASNGVVFDLVDAQSYEPEDLPKETLVL 97

Query: 2097 IIASLRKDERISRPTQVVAQWLSQRAADSNQQKLM-KACRFCVFGVGPQARRKWYNNLGR 1921
            I+AS  +D       +  A WL++   D     L+ K CRF VFGVG +A  + +N + +
Sbjct: 98   IVASTWEDGNPPSGARFFATWLAESVKDFRVGALLLKGCRFGVFGVGSRAYGERFNAVAK 157

Query: 1920 HFSICMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSDVCN 1765
             F   +R LG SE++PL E D +  +LD  F  W  K+V  LK  +  +  N
Sbjct: 158  GFVKQIRDLGGSEVVPLGEGDVDGGELDLDFEKWCEKVVGFLKGKVLENGAN 209


>KRH15213.1 hypothetical protein GLYMA_14G075000 [Glycine max]
          Length = 522

 Score =  114 bits (284), Expect = 8e-23
 Identities = 63/163 (38%), Positives = 98/163 (60%), Gaps = 3/163 (1%)
 Frame = -3

Query: 2238 KLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIASLRKDERI 2065
            K++  SETG  + L+ RL G L+ + +  DLVD + Y P+DLPKE+LI+++AS   D   
Sbjct: 50   KIIFVSETGTSKTLARRLHGLLASNDVAFDLVDAQHYEPEDLPKETLIILVASTWLDGAP 109

Query: 2064 SRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSICMRSLGA 1888
            S  ++  A WL+  AAD     L+ + CRF VFGVG +A    +N + R  +  +R+LGA
Sbjct: 110  SAASRFFATWLADAAADFRAGSLLLSRCRFAVFGVGSRAYGDSFNAVARGLAKHLRALGA 169

Query: 1887 SEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSDVCNEE 1759
            +E++PL E D +   +D VF  W  K+V+ LK+    + C+ +
Sbjct: 170  AEVVPLFEGDVDGGDVDAVFDRWCEKVVAVLKSGSVVESCDND 212


>XP_002281468.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase [Vitis vinifera]
          Length = 654

 Score =  114 bits (286), Expect = 9e-23
 Identities = 70/189 (37%), Positives = 106/189 (56%), Gaps = 4/189 (2%)
 Frame = -3

Query: 2256 SSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIASL 2083
            +S S+GKL   S+TG   +L+ RL   L+   ++ DLV  ++Y P+DL KE+L+LI+AS 
Sbjct: 47   NSSSKGKLFFVSQTGTSEILARRLLRLLTSCDLSFDLVHPKDYEPEDLSKETLVLIVAST 106

Query: 2082 RKDERISRPTQVVAQWLSQRAADSNQ-QKLMKACRFCVFGVGPQARRKWYNNLGRHFSIC 1906
             +D          + WL++ A D      L+  C+F VFGVG ++    +N + R FS  
Sbjct: 107  WEDGNPPPDAGFFSNWLAESADDFRVGSMLLSRCKFAVFGVGSRSYGAAFNAVARGFSKR 166

Query: 1905 MRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLK-ANITSDVCNEEMPYICVEGLK 1729
            MR LG  ++LP+EE D ++  LDEVF  WS KL+S LK  ++ + V +  M  +  +   
Sbjct: 167  MRKLGGLQVLPVEEGDVDAGDLDEVFDVWSRKLISVLKGGSVENGVFDGSMAVVESDAET 226

Query: 1728 FDEYNEDGD 1702
             D   ED D
Sbjct: 227  IDGSEEDYD 235


>XP_008377398.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Malus domestica]
          Length = 645

 Score =  114 bits (285), Expect = 1e-22
 Identities = 75/199 (37%), Positives = 105/199 (52%), Gaps = 13/199 (6%)
 Frame = -3

Query: 2259 SSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIAS 2086
            SSS  +GKL   S+TG    L+ RL   L+ + +  DLVD   Y P+DLPKE L+LI+AS
Sbjct: 42   SSSPRRGKLFFVSQTGTSEALARRLLDXLTSNGVAFDLVDPXNYEPEDLPKEXLVLIVAS 101

Query: 2085 LRKDERISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSI 1909
               D +     +  + WL++ A D     L+ + C+F VFGVG +A    +N +G+  + 
Sbjct: 102  TWDDGKPPPNARFFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGATFNAVGKDLAR 161

Query: 1908 CMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLK----------ANITSDVCNEE 1759
             MR+LGASE+ P+ E D +   LDEVF  WS K+V  LK           N   D C  E
Sbjct: 162  RMRALGASEMFPIWEXDVDGGDLDEVFDAWSGKVVRFLKGGAAENGGVLGNGVGDECEAE 221

Query: 1758 MPYICVEGLKFDEYNEDGD 1702
                  +G   DE +E+ D
Sbjct: 222  ----SFDGSDEDEEDEEED 236


>XP_009365568.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Pyrus x bretschneideri]
          Length = 641

 Score =  114 bits (284), Expect = 2e-22
 Identities = 66/159 (41%), Positives = 94/159 (59%), Gaps = 3/159 (1%)
 Frame = -3

Query: 2259 SSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIAS 2086
            SSS  +GKL   S+TG    L+ RL   L+ + +  DLVD   Y P+DLPKE+L+LI+AS
Sbjct: 42   SSSPRRGKLFFVSQTGTSEALARRLLDFLASNGVAFDLVDPNNYEPEDLPKETLVLIVAS 101

Query: 2085 LRKDERISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSI 1909
               D +     +  + WL++ A D     L+ + C+F VFGVG +A    +N +G+  + 
Sbjct: 102  TWDDGKPPPNARFFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGATFNAVGKDLAR 161

Query: 1908 CMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLK 1792
             MR+LGASE+ P+ E D +   LDEVF  WS K+V  LK
Sbjct: 162  RMRALGASEMFPIWEGDVDGGDLDEVFDAWSGKVVRFLK 200


>XP_003544413.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Glycine max] KRH15212.1 hypothetical
            protein GLYMA_14G075000 [Glycine max]
          Length = 642

 Score =  114 bits (284), Expect = 2e-22
 Identities = 63/163 (38%), Positives = 98/163 (60%), Gaps = 3/163 (1%)
 Frame = -3

Query: 2238 KLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIASLRKDERI 2065
            K++  SETG  + L+ RL G L+ + +  DLVD + Y P+DLPKE+LI+++AS   D   
Sbjct: 50   KIIFVSETGTSKTLARRLHGLLASNDVAFDLVDAQHYEPEDLPKETLIILVASTWLDGAP 109

Query: 2064 SRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSICMRSLGA 1888
            S  ++  A WL+  AAD     L+ + CRF VFGVG +A    +N + R  +  +R+LGA
Sbjct: 110  SAASRFFATWLADAAADFRAGSLLLSRCRFAVFGVGSRAYGDSFNAVARGLAKHLRALGA 169

Query: 1887 SEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSDVCNEE 1759
            +E++PL E D +   +D VF  W  K+V+ LK+    + C+ +
Sbjct: 170  AEVVPLFEGDVDGGDVDAVFDRWCEKVVAVLKSGSVVESCDND 212


>OAY80972.1 S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
            [Ananas comosus]
          Length = 663

 Score =  114 bits (284), Expect = 2e-22
 Identities = 67/163 (41%), Positives = 94/163 (57%), Gaps = 3/163 (1%)
 Frame = -3

Query: 2271 ITRVSSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLIL 2098
            I+  S S    K+   S TG    L+ RL   L    +  DLVD + Y P+DLPKE+L+L
Sbjct: 38   ISAPSPSNPNPKIFFASHTGTSETLARRLSHRLRSLGLPFDLVDPKHYEPEDLPKETLVL 97

Query: 2097 IIASLRKDERISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGR 1921
            I+AS  ++ R     + +A+WL++ A D     L+ + CRF VFGVG  A  + +N   R
Sbjct: 98   IVASTWEEGRPPPNAEFLARWLAESAEDFRVGSLLLSRCRFAVFGVGSGAYGEAFNAAAR 157

Query: 1920 HFSICMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLK 1792
             FS  MRSLGA+E+LP+ E D +   +DEVF  WS K++  LK
Sbjct: 158  DFSQWMRSLGATEMLPVSEGDVDGGDVDEVFDAWSEKVIGLLK 200


>XP_015884574.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Ziziphus jujuba]
          Length = 474

 Score =  112 bits (279), Expect = 2e-22
 Identities = 65/165 (39%), Positives = 95/165 (57%), Gaps = 3/165 (1%)
 Frame = -3

Query: 2259 SSSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIAS 2086
            SSS  + KL   S+TG  + L+ RL   L+ + +  DLVD   Y P+DLPKE+L+LI+AS
Sbjct: 48   SSSPRKAKLFFISQTGTSKALAVRLFDLLTSNNLAFDLVDPNHYEPEDLPKENLVLIVAS 107

Query: 2085 LRKDERISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSI 1909
              +D +     +  A WLS+ + D     L+ + C+F VFGVG +A    +N + +  S 
Sbjct: 108  TWEDGKAPPTAKFFANWLSESSDDFRVGSLLLSQCKFAVFGVGSRAYGATFNAVAKDVSK 167

Query: 1908 CMRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLKANITSD 1774
             MR+LGA EILP+ E D +   +DEVF  WS K+V   K  +  +
Sbjct: 168  QMRALGAKEILPVWEGDVDGGDIDEVFDAWSGKVVGFCKGGVVEN 212


>XP_009336348.1 PREDICTED: S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine
            synthase-like [Pyrus x bretschneideri]
          Length = 644

 Score =  112 bits (281), Expect = 4e-22
 Identities = 76/197 (38%), Positives = 103/197 (52%), Gaps = 13/197 (6%)
 Frame = -3

Query: 2256 SSKSQGKLLITSETG--RVLSHRLQGTLSDSLINVDLVDTEEYCPDDLPKESLILIIASL 2083
            SS  +GKL   S+TG    L+ RL   L    +  DLVD   Y P+DLPKE+L+LI+AS 
Sbjct: 43   SSPRRGKLFFVSQTGTSEALARRLLDFLKLKGVAFDLVDPNNYEPEDLPKETLVLIVAST 102

Query: 2082 RKDERISRPTQVVAQWLSQRAADSNQQKLMKA-CRFCVFGVGPQARRKWYNNLGRHFSIC 1906
              D +     +  + WL++ A D     L+ + C+F VFGVG +A    +N +G+  +  
Sbjct: 103  WDDGKPPPNAKFFSNWLAESAEDFRVGSLLLSNCKFAVFGVGSRAYGATFNAVGKDLARR 162

Query: 1905 MRSLGASEILPLEEADQESEKLDEVFSCWSTKLVSNLK----------ANITSDVCNEEM 1756
            MR+LGASE+ P+ E D +   LDEVF  WS K+V  LK           N  SD C  E 
Sbjct: 163  MRALGASEMFPIWEGDVDGGDLDEVFDAWSGKVVRFLKGGAAENGGVLGNGVSDECEAES 222

Query: 1755 PYICVEGLKFDEYNEDG 1705
              +     K DE  EDG
Sbjct: 223  FDV---SDKEDEEEEDG 236