BLASTX nr result
ID: Akebia27_contig00043508
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Akebia27_contig00043508
(376 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN65591.1| hypothetical protein VITISV_042091 [Vitis vinifera] 142 5e-32
emb|CAB46043.1| retrotransposon like protein [Arabidopsis thalia... 117 2e-24
gb|AAK43485.1|AC084807_10 polyprotein, putative [Arabidopsis tha... 115 8e-24
emb|CAN66496.1| hypothetical protein VITISV_036886 [Vitis vinifera] 114 2e-23
ref|XP_007014689.1| Uncharacterized protein TCM_040047 [Theobrom... 112 5e-23
gb|AAT39281.2| Integrase core domain containing protein [Solanum... 112 5e-23
emb|CAN68578.1| hypothetical protein VITISV_019283 [Vitis vinifera] 111 9e-23
emb|CAN70817.1| hypothetical protein VITISV_029336 [Vitis vinifera] 110 3e-22
emb|CAN62447.1| hypothetical protein VITISV_024007 [Vitis vinifera] 109 3e-22
dbj|BAB10876.1| polyprotein [Arabidopsis thaliana] 109 4e-22
gb|AAC02666.1| polyprotein [Arabidopsis thaliana] 108 6e-22
gb|AAC02672.1| polyprotein [Arabidopsis arenosa] 108 6e-22
gb|AAC02664.1| polyprotein [Arabidopsis thaliana] 108 6e-22
gb|AAC02669.1| polyprotein [Arabidopsis thaliana] 108 6e-22
dbj|BAA78426.1| polyprotein [Arabidopsis thaliana] 108 8e-22
dbj|BAA78424.1| polyprotein [Arabidopsis thaliana] gi|404433798|... 108 8e-22
dbj|BAM42650.1| Polyprotein [Arabidopsis thaliana] 108 8e-22
dbj|BAM42649.1| Polyprotein [Arabidopsis thaliana] 108 8e-22
dbj|BAM42647.1| polyprotein [Arabidopsis thaliana] gi|403406144|... 108 8e-22
dbj|BAM42646.1| polyprotein [Arabidopsis thaliana] 108 8e-22
>emb|CAN65591.1| hypothetical protein VITISV_042091 [Vitis vinifera]
Length = 1427
Score = 142 bits (358), Expect = 5e-32
Identities = 73/128 (57%), Positives = 89/128 (69%), Gaps = 4/128 (3%)
Frame = -1
Query: 373 SLVMVQDLTTGTILARGRSKNDVYEWPSLQDSNSVG---KHAYVGVKTSIDCWHDRLGHP 203
S +++DL TG L +GRSK+DVYEWP N++G K A V VKTS+ WH RLGHP
Sbjct: 399 SSFVIKDLKTGARLTQGRSKDDVYEWPWPNKGNTLGTSPKQACVSVKTSLANWHHRLGHP 458
Query: 202 STKILNHIISHFKLPIQLNHSKFSF-CDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWG 26
S++I +I L I S +F C+SC CNKSH+LPFGVSSL+SR LDL+YSDVWG
Sbjct: 459 SSRIFQFLIRKHNLQIYPTESFHNFFCESCLCNKSHKLPFGVSSLRSRGXLDLVYSDVWG 518
Query: 25 PSPIRSID 2
PSPI SID
Sbjct: 519 PSPIESID 526
>emb|CAB46043.1| retrotransposon like protein [Arabidopsis thaliana]
gi|7268438|emb|CAB80958.1| retrotransposon like protein
[Arabidopsis thaliana]
Length = 1474
Score = 117 bits (293), Expect = 2e-24
Identities = 56/120 (46%), Positives = 80/120 (66%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL TGT+L +GR+K+++YEWP + + + KT++ WH RLGHPS+ ILN
Sbjct: 424 VKDLNTGTLLLQGRTKDELYEWP-VTNPKATALFTTPSPKTTLSSWHSRLGHPSSSILNT 482
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS F LP+ ++ S C C NKSH+LPF +SS++S PL+ ++SDVW SPI S D
Sbjct: 483 LISKFSLPVSVSASNKLACSDCFINKSHKLPFSISSIKSTSPLEYIFSDVW-MSPILSPD 541
>gb|AAK43485.1|AC084807_10 polyprotein, putative [Arabidopsis thaliana]
gi|225898008|dbj|BAH30336.1| hypothetical protein
[Arabidopsis thaliana]
Length = 1459
Score = 115 bits (287), Expect = 8e-24
Identities = 55/120 (45%), Positives = 77/120 (64%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL TGT+L +GR+K+D+YEWP + + + KT++ WH RLGHPS ILN
Sbjct: 429 VKDLNTGTLLLQGRTKDDLYEWP-VTNPPATALFTSPSPKTTLSSWHSRLGHPSASILNT 487
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
++S F LP+ + S + C C NKSH+LPF SS+ S PL+ +++DVW SPI S D
Sbjct: 488 LLSKFSLPVSVASSNKTSCSDCLINKSHKLPFATSSIHSSSPLEYIFTDVW-TSPIISHD 546
>emb|CAN66496.1| hypothetical protein VITISV_036886 [Vitis vinifera]
Length = 1092
Score = 114 bits (284), Expect = 2e-23
Identities = 55/121 (45%), Positives = 73/121 (60%)
Frame = -1
Query: 364 MVQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILN 185
+V+D TG L RGR +N VY S + G + + DCWH RLGH + KIL+
Sbjct: 291 LVKDRMTGAXLMRGRCENGVYPVALQSSSPQXNQIHLAGTRVTFDCWHHRLGHXTPKILS 350
Query: 184 HIISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSI 5
++ LP+ + S S C SCQCNKSH+LPF +SL++ L+ LY DVWGPSP+ SI
Sbjct: 351 SLLQSHDLPVASSKSTLS-CISCQCNKSHKLPFSSTSLKNXVXLEFLYDDVWGPSPVSSI 409
Query: 4 D 2
D
Sbjct: 410 D 410
>ref|XP_007014689.1| Uncharacterized protein TCM_040047 [Theobroma cacao]
gi|508785052|gb|EOY32308.1| Uncharacterized protein
TCM_040047 [Theobroma cacao]
Length = 678
Score = 112 bits (280), Expect = 5e-23
Identities = 54/121 (44%), Positives = 78/121 (64%)
Frame = -1
Query: 364 MVQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILN 185
+V+D + G L R ++N +YE P S+ ++ VKTS D WH RLGHPS++IL
Sbjct: 132 LVKDSSMGAPLVRSLNRNGLYELPRCMSHLSMITTTFLSVKTSFDVWHRRLGHPSSEILT 191
Query: 184 HIISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSI 5
++ F LP ++ FS C+SC CN+SH+LPFG S+L S + L LLY+DVWGP+ I +
Sbjct: 192 FMLKFFGLPSIASNKAFS-CNSCACNESHKLPFGQSTLSSTKHLQLLYTDVWGPAQISFV 250
Query: 4 D 2
+
Sbjct: 251 E 251
>gb|AAT39281.2| Integrase core domain containing protein [Solanum demissum]
Length = 1760
Score = 112 bits (280), Expect = 5e-23
Identities = 56/121 (46%), Positives = 77/121 (63%), Gaps = 1/121 (0%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL+TG L RG++++ +YEWP S V + WH RLGHP+ + LN
Sbjct: 401 VKDLSTGAPLFRGQNRDGLYEWPL----GSAHHTPQCNVVVPLHLWHRRLGHPNHRTLNM 456
Query: 181 IISHFKLPIQLNHSKF-SFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSI 5
I F LP+ +HS+ S C+SC NK HRLPF +SLQS+ PL ++Y+D+WGPSP+ SI
Sbjct: 457 IFHQFSLPV--SHSRTASICNSCYSNKMHRLPFSENSLQSQRPLQIIYTDLWGPSPVLSI 514
Query: 4 D 2
D
Sbjct: 515 D 515
>emb|CAN68578.1| hypothetical protein VITISV_019283 [Vitis vinifera]
Length = 472
Score = 111 bits (278), Expect = 9e-23
Identities = 49/111 (44%), Positives = 73/111 (65%)
Frame = -1
Query: 334 LARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNHIISHFKLPI 155
L RG +K +VYEW + +++ HA++ K+++ WH RLGHPS+++L ++S LP+
Sbjct: 6 LVRGXNKGNVYEWSTSSTTSTTKPHAFLANKSTMKSWHCRLGHPSSQVLTKLVSTQSLPM 65
Query: 154 QLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
S CDS NKSH+LPFG SS+ PL+++Y+DVWGP+PI S D
Sbjct: 66 SAPRIFSSHCDSFLSNKSHKLPFGASSISCTRPLEIIYTDVWGPAPIISYD 116
>emb|CAN70817.1| hypothetical protein VITISV_029336 [Vitis vinifera]
Length = 1044
Score = 110 bits (274), Expect = 3e-22
Identities = 54/110 (49%), Positives = 73/110 (66%), Gaps = 1/110 (0%)
Frame = -1
Query: 349 TTGTILARGRSKNDVYEWPSLQDSNSVGKH-AYVGVKTSIDCWHDRLGHPSTKILNHIIS 173
+TG IL RG +N VY +P+ + S K AYV +TSID WH RLGHPS K++ ++++
Sbjct: 249 STGVILLRGACENGVYIFPNSMVAPSTPKMVAYVHERTSIDGWHKRLGHPSIKVVQNLVN 308
Query: 172 HFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGP 23
F LP+ N S C SC NK+H+ PFG +S QS PL+++YSDVWGP
Sbjct: 309 LFSLPLTTNKLPLS-CPSCSINKAHQQPFGSTSFQSHSPLEIIYSDVWGP 357
>emb|CAN62447.1| hypothetical protein VITISV_024007 [Vitis vinifera]
Length = 1144
Score = 109 bits (273), Expect = 3e-22
Identities = 53/117 (45%), Positives = 77/117 (65%), Gaps = 1/117 (0%)
Frame = -1
Query: 349 TTGTILARGRSKNDVYEWPSLQDSNSVGKH-AYVGVKTSIDCWHDRLGHPSTKILNHIIS 173
+TG IL RG +N VY +P+ + S K AYV +TSI+ WH RLGHPS K++ ++++
Sbjct: 373 STGAILLRGACENGVYIFPNSMMAPSTPKMVAYVHERTSINGWHKRLGHPSIKVVQNLVN 432
Query: 172 HFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
F LP+ N S C SC NK+H+ PFG +S QS PL+++YSDVWGP+ + ++
Sbjct: 433 LFSLPLTTNKLPLS-CPSCSINKAHQQPFGSTSFQSHSPLEIIYSDVWGPAHVTGLN 488
>dbj|BAB10876.1| polyprotein [Arabidopsis thaliana]
Length = 1429
Score = 109 bits (272), Expect = 4e-22
Identities = 54/120 (45%), Positives = 75/120 (62%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL TG L +GR++N++YEWP Q S ++ A KT + WH RLGHP+ IL
Sbjct: 406 VKDLNTGARLLQGRTRNELYEWPVNQKSITI-LTASPSPKTDLSSWHQRLGHPALPILKD 464
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
++SHF LP+ K C C NKSH+LPF +++ S +PL+ LY+DVW SP S+D
Sbjct: 465 VVSHFHLPLSNTIPKQLPCSDCSINKSHKLPFFTNTIVSSQPLEYLYTDVW-TSPCISVD 523
>gb|AAC02666.1| polyprotein [Arabidopsis thaliana]
Length = 1451
Score = 108 bits (271), Expect = 6e-22
Identities = 56/120 (46%), Positives = 74/120 (61%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL+TG L +G++KN++YEWP + S + A KT + WH RLGHPS IL
Sbjct: 425 VKDLSTGARLLQGKTKNELYEWP-VNSSIATSMFASPTPKTDLPSWHARLGHPSLPILKA 483
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS F LPI + C C NKSH+LPF +++ S PL+ LY+DVW SPI SID
Sbjct: 484 LISKFSLPISHSLQNQLLCSDCSINKSHKLPFYSNTIASSHPLEYLYTDVW-TSPITSID 542
>gb|AAC02672.1| polyprotein [Arabidopsis arenosa]
Length = 1390
Score = 108 bits (271), Expect = 6e-22
Identities = 56/120 (46%), Positives = 74/120 (61%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL+TG L +G++KN++YEWP + S + A KT + WH RLGHPS IL
Sbjct: 425 VKDLSTGARLLQGKTKNELYEWP-VNSSIATSMFASPTPKTDLPSWHARLGHPSLPILKT 483
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS F LPI + C C NKSH+LPF +++ S PL+ LY+DVW SPI SID
Sbjct: 484 LISKFSLPISHSLQNQLLCSDCSINKSHKLPFYSNTIASSHPLEYLYTDVW-TSPITSID 542
>gb|AAC02664.1| polyprotein [Arabidopsis thaliana]
Length = 1451
Score = 108 bits (271), Expect = 6e-22
Identities = 56/120 (46%), Positives = 74/120 (61%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL+TG L +G++KN++YEWP + S + A KT + WH RLGHPS IL
Sbjct: 425 VKDLSTGARLLQGKTKNELYEWP-VNSSIATSMFASPTPKTDLPSWHARLGHPSLPILKA 483
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS F LPI + C C NKSH+LPF +++ S PL+ LY+DVW SPI SID
Sbjct: 484 LISKFSLPISHSLQNQLLCSDCSINKSHKLPFYSNTIASSHPLEYLYTDVW-TSPITSID 542
>gb|AAC02669.1| polyprotein [Arabidopsis thaliana]
Length = 1451
Score = 108 bits (271), Expect = 6e-22
Identities = 56/120 (46%), Positives = 74/120 (61%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL+TG L +G++KN++YEWP + S + A KT + WH RLGHPS IL
Sbjct: 425 VKDLSTGARLLQGKTKNELYEWP-VNSSIATSMFASPTPKTDLPSWHARLGHPSLPILKA 483
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS F LPI + C C NKSH+LPF +++ S PL+ LY+DVW SPI SID
Sbjct: 484 LISKFSLPISHSLQNQLLCSDCSINKSHKLPFYSNTIASSHPLEYLYTDVW-TSPITSID 542
>dbj|BAA78426.1| polyprotein [Arabidopsis thaliana]
Length = 1475
Score = 108 bits (270), Expect = 8e-22
Identities = 55/120 (45%), Positives = 75/120 (62%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL TG L +G++K+++YEWP + S +V A K + WH RLGHPS ILN
Sbjct: 421 VKDLNTGVPLLQGKTKDELYEWP-IASSQAVSMFASPCSKATHSSWHSRLGHPSLAILNS 479
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS+ LP+ K C C NKSH++PF S++ S +PL+ +YSDVW SPI SID
Sbjct: 480 VISNHSLPVLNPSHKLLSCSDCFINKSHKVPFSNSTITSSKPLEYIYSDVWS-SPILSID 538
>dbj|BAA78424.1| polyprotein [Arabidopsis thaliana] gi|404433798|dbj|BAM44533.1|
polyprotein [Arabidopsis thaliana]
Length = 1330
Score = 108 bits (270), Expect = 8e-22
Identities = 55/120 (45%), Positives = 75/120 (62%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL TG L +G++K+++YEWP + S +V A K + WH RLGHPS ILN
Sbjct: 276 VKDLNTGVPLLQGKTKDELYEWP-IASSQAVSMFASPCSKATHSSWHSRLGHPSLAILNS 334
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS+ LP+ K C C NKSH++PF S++ S +PL+ +YSDVW SPI SID
Sbjct: 335 VISNHSLPVLNPSHKLLSCSDCFINKSHKVPFSNSTITSSKPLEYIYSDVWS-SPILSID 393
>dbj|BAM42650.1| Polyprotein [Arabidopsis thaliana]
Length = 1475
Score = 108 bits (270), Expect = 8e-22
Identities = 55/120 (45%), Positives = 75/120 (62%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL TG L +G++K+++YEWP + S +V A K + WH RLGHPS ILN
Sbjct: 421 VKDLNTGVPLLQGKTKDELYEWP-IASSQAVSMFASPCSKATHSSWHSRLGHPSLAILNS 479
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS+ LP+ K C C NKSH++PF S++ S +PL+ +YSDVW SPI SID
Sbjct: 480 VISNHSLPVLNPSHKLLSCSDCFINKSHKVPFSNSTITSSKPLEYIYSDVWS-SPILSID 538
>dbj|BAM42649.1| Polyprotein [Arabidopsis thaliana]
Length = 1475
Score = 108 bits (270), Expect = 8e-22
Identities = 55/120 (45%), Positives = 75/120 (62%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL TG L +G++K+++YEWP + S +V A K + WH RLGHPS ILN
Sbjct: 421 VKDLNTGVPLLQGKTKDELYEWP-IASSQAVSMFASPCSKATHSSWHSRLGHPSLAILNS 479
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS+ LP+ K C C NKSH++PF S++ S +PL+ +YSDVW SPI SID
Sbjct: 480 VISNHSLPVLNPSHKLLSCSDCFINKSHKVPFSNSTITSSKPLEYIYSDVWS-SPILSID 538
>dbj|BAM42647.1| polyprotein [Arabidopsis thaliana] gi|403406144|dbj|BAM42648.1|
polyprotein [Arabidopsis thaliana]
Length = 1475
Score = 108 bits (270), Expect = 8e-22
Identities = 55/120 (45%), Positives = 75/120 (62%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL TG L +G++K+++YEWP + S +V A K + WH RLGHPS ILN
Sbjct: 421 VKDLNTGVPLLQGKTKDELYEWP-IASSQAVSMFASPCSKATHSSWHSRLGHPSLAILNS 479
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS+ LP+ K C C NKSH++PF S++ S +PL+ +YSDVW SPI SID
Sbjct: 480 VISNHSLPVLNPSHKLLSCSDCFINKSHKVPFSNSTITSSKPLEYIYSDVWS-SPILSID 538
>dbj|BAM42646.1| polyprotein [Arabidopsis thaliana]
Length = 1475
Score = 108 bits (270), Expect = 8e-22
Identities = 55/120 (45%), Positives = 75/120 (62%)
Frame = -1
Query: 361 VQDLTTGTILARGRSKNDVYEWPSLQDSNSVGKHAYVGVKTSIDCWHDRLGHPSTKILNH 182
V+DL TG L +G++K+++YEWP + S +V A K + WH RLGHPS ILN
Sbjct: 421 VKDLNTGVPLLQGKTKDELYEWP-IASSQAVSMFASPCSKATHSSWHSRLGHPSLAILNS 479
Query: 181 IISHFKLPIQLNHSKFSFCDSCQCNKSHRLPFGVSSLQSREPLDLLYSDVWGPSPIRSID 2
+IS+ LP+ K C C NKSH++PF S++ S +PL+ +YSDVW SPI SID
Sbjct: 480 VISNHSLPVLNPSHKLLSCSDCFINKSHKVPFSNSTITSSKPLEYIYSDVWS-SPILSID 538