BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia26_contig00030515
         (268 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_007030504.1| Intracellular protein transport protein USO1...   143   2e-32
ref|XP_007208094.1| hypothetical protein PRUPE_ppa001296mg [Prun...   141   1e-31
ref|XP_006577299.1| PREDICTED: uncharacterized protein LOC100800...   140   2e-31
gb|EXB93210.1| hypothetical protein L484_024549 [Morus notabilis]     140   2e-31
ref|XP_006347726.1| PREDICTED: intracellular protein transport p...   139   3e-31
ref|XP_006347724.1| PREDICTED: intracellular protein transport p...   139   3e-31
ref|XP_004230077.1| PREDICTED: uncharacterized protein LOC101255...   139   3e-31
ref|XP_006370993.1| hypothetical protein POPTR_0019s02440g [Popu...   139   5e-31
ref|XP_002263011.1| PREDICTED: uncharacterized protein LOC100258...   138   7e-31
ref|XP_002319088.1| hypothetical protein POPTR_0013s04060g [Popu...   138   9e-31
gb|EYU26862.1| hypothetical protein MIMGU_mgv1a001409mg [Mimulus...   137   2e-30
ref|XP_007147191.1| hypothetical protein PHAVU_006G103500g [Phas...   137   2e-30
ref|XP_004302068.1| PREDICTED: uncharacterized protein LOC101309...   135   8e-30
ref|XP_002521947.1| hypothetical protein RCOM_0607680 [Ricinus c...   132   7e-29
ref|XP_004139387.1| PREDICTED: uncharacterized protein LOC101220...   131   9e-29
ref|XP_006487767.1| PREDICTED: putative leucine-rich repeat-cont...   130   2e-28
emb|CBI17960.3| unnamed protein product [Vitis vinifera]              129   6e-28
emb|CAN75727.1| hypothetical protein VITISV_031407 [Vitis vinifera]   129   6e-28
ref|XP_006423971.1| hypothetical protein CICLE_v10027718mg [Citr...   128   9e-28
ref|XP_006443890.1| hypothetical protein CICLE_v10018827mg [Citr...   125   6e-27

>ref|XP_007030504.1| Intracellular protein transport protein USO1, putative [Theobroma
           cacao] gi|508719109|gb|EOY11006.1| Intracellular protein
           transport protein USO1, putative [Theobroma cacao]
          Length = 846

 Score =  143 bits (361), Expect = 2e-32
 Identities = 64/88 (72%), Positives = 75/88 (85%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA  C+LQ PCLLCSRLD+VLG EK  FYWDL+CG HK EISSL +CH H+KLV+VHG+C
Sbjct: 86  FAHKCKLQTPCLLCSRLDHVLGKEKQKFYWDLVCGDHKLEISSLVYCHSHNKLVDVHGMC 145

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           E+CLFSF+  +KSN+ETYRLLVGKLG D
Sbjct: 146 ESCLFSFATINKSNAETYRLLVGKLGED 173


>ref|XP_007208094.1| hypothetical protein PRUPE_ppa001296mg [Prunus persica]
           gi|462403736|gb|EMJ09293.1| hypothetical protein
           PRUPE_ppa001296mg [Prunus persica]
          Length = 861

 Score =  141 bits (355), Expect = 1e-31
 Identities = 63/88 (71%), Positives = 74/88 (84%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA +C LQ PCLLCSRLD+VLG EK G+YWDL CG HKSEISSL  C+ H KLV+VHG+C
Sbjct: 48  FAYYCGLQTPCLLCSRLDHVLGKEKLGYYWDLFCGNHKSEISSLVLCYAHHKLVDVHGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           E+CLFSF+  ++SN+ETYRLLVGKLG D
Sbjct: 108 ESCLFSFATINRSNAETYRLLVGKLGDD 135


>ref|XP_006577299.1| PREDICTED: uncharacterized protein LOC100800595 isoform X2 [Glycine
           max] gi|571447153|ref|XP_006577300.1| PREDICTED:
           uncharacterized protein LOC100800595 isoform X3 [Glycine
           max] gi|571447155|ref|XP_003521761.2| PREDICTED:
           uncharacterized protein LOC100800595 isoform X1 [Glycine
           max] gi|571447157|ref|XP_006577301.1| PREDICTED:
           uncharacterized protein LOC100800595 isoform X4 [Glycine
           max]
          Length = 864

 Score =  140 bits (353), Expect = 2e-31
 Identities = 63/86 (73%), Positives = 73/86 (84%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA +C+LQIPCLLCSRLD+VLG EK G+YWDLIC  HK+EIS L  C  HDKLVNV G+C
Sbjct: 49  FAGYCKLQIPCLLCSRLDHVLGKEKGGYYWDLICSGHKTEISYLVLCCAHDKLVNVQGMC 108

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLG 11
           E+CLFSF+  +KSN+ETYRLLVGKLG
Sbjct: 109 ESCLFSFATINKSNAETYRLLVGKLG 134


>gb|EXB93210.1| hypothetical protein L484_024549 [Morus notabilis]
          Length = 938

 Score =  140 bits (352), Expect = 2e-31
 Identities = 63/89 (70%), Positives = 76/89 (85%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FAR C+LQ PCLLCSRLD+VLG EK G+YWDL+C  HKSEISSL  CH H+KLV+VH +C
Sbjct: 49  FARGCKLQTPCLLCSRLDHVLGKEKVGYYWDLMCKNHKSEISSLVLCHAHNKLVDVHKMC 108

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTDL 2
           E+CLFSF+  +KSN+ETYRLLVGKLG ++
Sbjct: 109 ESCLFSFATINKSNAETYRLLVGKLGEEV 137


>ref|XP_006347726.1| PREDICTED: intracellular protein transport protein USO1-like
           isoform X3 [Solanum tuberosum]
          Length = 860

 Score =  139 bits (351), Expect = 3e-31
 Identities = 60/88 (68%), Positives = 76/88 (86%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA++C+LQ+PCLLCSRLD+VLG E++GFYW+LIC  HK  ISSL  CH H+ LV+VHG+C
Sbjct: 48  FAQYCQLQVPCLLCSRLDHVLGKERAGFYWELICPNHKYRISSLVLCHNHNNLVDVHGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           E+CLFSF+  +KSN+ETYRLLVGKLG +
Sbjct: 108 ESCLFSFATVNKSNAETYRLLVGKLGAE 135


>ref|XP_006347724.1| PREDICTED: intracellular protein transport protein USO1-like
           isoform X1 [Solanum tuberosum]
           gi|565361970|ref|XP_006347725.1| PREDICTED:
           intracellular protein transport protein USO1-like
           isoform X2 [Solanum tuberosum]
          Length = 861

 Score =  139 bits (351), Expect = 3e-31
 Identities = 60/88 (68%), Positives = 76/88 (86%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA++C+LQ+PCLLCSRLD+VLG E++GFYW+LIC  HK  ISSL  CH H+ LV+VHG+C
Sbjct: 48  FAQYCQLQVPCLLCSRLDHVLGKERAGFYWELICPNHKYRISSLVLCHNHNNLVDVHGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           E+CLFSF+  +KSN+ETYRLLVGKLG +
Sbjct: 108 ESCLFSFATVNKSNAETYRLLVGKLGAE 135


>ref|XP_004230077.1| PREDICTED: uncharacterized protein LOC101255631 [Solanum
           lycopersicum]
          Length = 910

 Score =  139 bits (351), Expect = 3e-31
 Identities = 60/88 (68%), Positives = 76/88 (86%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA++C+LQ+PCLLCSRLD+VLG E++GFYW+LIC  HK  ISSL  CH H+ LV+VHG+C
Sbjct: 48  FAQYCQLQVPCLLCSRLDHVLGKERAGFYWELICPNHKYRISSLVLCHNHNNLVDVHGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           E+CLFSF+  +KSN+ETYRLLVGKLG +
Sbjct: 108 ESCLFSFATVNKSNAETYRLLVGKLGAE 135


>ref|XP_006370993.1| hypothetical protein POPTR_0019s02440g [Populus trichocarpa]
           gi|550316576|gb|ERP48790.1| hypothetical protein
           POPTR_0019s02440g [Populus trichocarpa]
          Length = 891

 Score =  139 bits (349), Expect = 5e-31
 Identities = 63/88 (71%), Positives = 73/88 (82%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA   ELQ PCLLCSRLD++LG++   +YWDLICG HK EISSL FCH H+ LVNVHG+C
Sbjct: 48  FACQWELQTPCLLCSRLDHILGSKNLKYYWDLICGNHKLEISSLVFCHAHNNLVNVHGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           ENCLFSF+  +KSN+ETYRLLVGKLG D
Sbjct: 108 ENCLFSFATTNKSNAETYRLLVGKLGED 135


>ref|XP_002263011.1| PREDICTED: uncharacterized protein LOC100258654 [Vitis vinifera]
          Length = 877

 Score =  138 bits (348), Expect = 7e-31
 Identities = 65/88 (73%), Positives = 70/88 (79%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FAR CELQ PCLLCSRLD VLG EK GFYWDLIC  HK EISSL  C+ H KLVN  G+C
Sbjct: 45  FARSCELQAPCLLCSRLDRVLGKEKLGFYWDLICHNHKLEISSLVLCYTHKKLVNGRGMC 104

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           ENCLFSF+  +K N+ETYRLLVGKLG D
Sbjct: 105 ENCLFSFATINKYNAETYRLLVGKLGED 132


>ref|XP_002319088.1| hypothetical protein POPTR_0013s04060g [Populus trichocarpa]
           gi|222857464|gb|EEE95011.1| hypothetical protein
           POPTR_0013s04060g [Populus trichocarpa]
          Length = 954

 Score =  138 bits (347), Expect = 9e-31
 Identities = 62/88 (70%), Positives = 73/88 (82%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA   ELQ PCLLCSRLD++LG+ K  +YWDLICG HK E+SSL FCH H+ LV+VHG+C
Sbjct: 48  FACQWELQTPCLLCSRLDHILGSRKLRYYWDLICGNHKLEVSSLVFCHAHNNLVDVHGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           ENCLFSF+  +KSN+ETYRLLVGKLG D
Sbjct: 108 ENCLFSFATTNKSNAETYRLLVGKLGED 135


>gb|EYU26862.1| hypothetical protein MIMGU_mgv1a001409mg [Mimulus guttatus]
          Length = 825

 Score =  137 bits (345), Expect = 2e-30
 Identities = 61/88 (69%), Positives = 72/88 (81%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FAR+C+LQIPCLLCSRLD+VLGNEK  F+WDL C  HK  ISSL  C  H+KLV+VHG C
Sbjct: 59  FARYCQLQIPCLLCSRLDHVLGNEKDSFHWDLFCHNHKLNISSLVLCQHHNKLVDVHGTC 118

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           E C FSF+  +KSN+ETYRLLVGKLG++
Sbjct: 119 ETCFFSFATTNKSNAETYRLLVGKLGSE 146


>ref|XP_007147191.1| hypothetical protein PHAVU_006G103500g [Phaseolus vulgaris]
           gi|593693342|ref|XP_007147192.1| hypothetical protein
           PHAVU_006G103500g [Phaseolus vulgaris]
           gi|593693344|ref|XP_007147193.1| hypothetical protein
           PHAVU_006G103500g [Phaseolus vulgaris]
           gi|593693346|ref|XP_007147194.1| hypothetical protein
           PHAVU_006G103500g [Phaseolus vulgaris]
           gi|561020414|gb|ESW19185.1| hypothetical protein
           PHAVU_006G103500g [Phaseolus vulgaris]
           gi|561020415|gb|ESW19186.1| hypothetical protein
           PHAVU_006G103500g [Phaseolus vulgaris]
           gi|561020416|gb|ESW19187.1| hypothetical protein
           PHAVU_006G103500g [Phaseolus vulgaris]
           gi|561020417|gb|ESW19188.1| hypothetical protein
           PHAVU_006G103500g [Phaseolus vulgaris]
          Length = 852

 Score =  137 bits (344), Expect = 2e-30
 Identities = 60/85 (70%), Positives = 71/85 (83%)
 Frame = -1

Query: 265 ARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLCE 86
           A +C+LQ PCL CSRLD+VLG E+ G+YWDLIC  HK+EISSL  C  HDKLVNV G+CE
Sbjct: 50  AGYCKLQTPCLFCSRLDHVLGKERDGYYWDLICSGHKTEISSLVLCRAHDKLVNVQGMCE 109

Query: 85  NCLFSFSMDHKSNSETYRLLVGKLG 11
           +CLFSF+  +KSN+ETYRLLVGKLG
Sbjct: 110 SCLFSFATVNKSNAETYRLLVGKLG 134


>ref|XP_004302068.1| PREDICTED: uncharacterized protein LOC101309555 [Fragaria vesca
           subsp. vesca]
          Length = 901

 Score =  135 bits (339), Expect = 8e-30
 Identities = 60/86 (69%), Positives = 74/86 (86%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA +C LQ PCL+CSRLD+V GNEK G+Y DLICG+HKSEISSL  C  H+KLV+V+G+C
Sbjct: 46  FAHYCGLQTPCLMCSRLDHVFGNEKPGYYRDLICGSHKSEISSLVLCRAHNKLVDVNGMC 105

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLG 11
           E+CLFSF+  ++SN+ETYRLLVGKLG
Sbjct: 106 ESCLFSFATINRSNAETYRLLVGKLG 131


>ref|XP_002521947.1| hypothetical protein RCOM_0607680 [Ricinus communis]
           gi|223538751|gb|EEF40351.1| hypothetical protein
           RCOM_0607680 [Ricinus communis]
          Length = 1162

 Score =  132 bits (331), Expect = 7e-29
 Identities = 60/86 (69%), Positives = 73/86 (84%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA + ELQ PCLLCSRLD++LGN++  +Y DLICG HK EISSL  CH H+KLV+VHG+C
Sbjct: 48  FACYYELQTPCLLCSRLDHILGNKRLKYYRDLICGKHKLEISSLVLCHAHNKLVDVHGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLG 11
           E+CLFSF+  +KSN+ETYRLLVGKLG
Sbjct: 108 ESCLFSFATINKSNAETYRLLVGKLG 133


>ref|XP_004139387.1| PREDICTED: uncharacterized protein LOC101220667 [Cucumis sativus]
           gi|449519000|ref|XP_004166523.1| PREDICTED:
           uncharacterized protein LOC101225941 [Cucumis sativus]
          Length = 904

 Score =  131 bits (330), Expect = 9e-29
 Identities = 59/87 (67%), Positives = 69/87 (79%)
 Frame = -1

Query: 265 ARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLCE 86
           A F +L  PCLLCSRLD++ G+EK G+ W+LIC  HK E+SSL  CH H+KLVNVH +CE
Sbjct: 45  AHFWKLHTPCLLCSRLDHIFGSEKRGYVWNLICSKHKVELSSLVLCHAHNKLVNVHEMCE 104

Query: 85  NCLFSFSMDHKSNSETYRLLVGKLGTD 5
           NCLFSF+   KSNSETYRLLVGKLG D
Sbjct: 105 NCLFSFATFKKSNSETYRLLVGKLGED 131


>ref|XP_006487767.1| PREDICTED: putative leucine-rich repeat-containing protein
           DDB_G0290503-like isoform X1 [Citrus sinensis]
           gi|568869077|ref|XP_006487768.1| PREDICTED: putative
           leucine-rich repeat-containing protein DDB_G0290503-like
           isoform X2 [Citrus sinensis]
          Length = 1099

 Score =  130 bits (327), Expect = 2e-28
 Identities = 60/88 (68%), Positives = 72/88 (81%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA +CELQ PCLLCSRLD+VLGNEK   Y DLICG H+ EISSL  CH H+KLV+V G+C
Sbjct: 48  FAHYCELQRPCLLCSRLDHVLGNEKLNPYRDLICGKHRLEISSLVLCHAHNKLVDVRGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           +NCLFSF+  ++S +ETYRLLVGK+G D
Sbjct: 108 QNCLFSFATVNRSITETYRLLVGKVGDD 135


>emb|CBI17960.3| unnamed protein product [Vitis vinifera]
          Length = 754

 Score =  129 bits (323), Expect = 6e-28
 Identities = 61/89 (68%), Positives = 73/89 (82%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           F+R+C+LQ PC LCSRLD+VLGNE+  FY +L+CG H+SE+SSL  C IHDKL +VHG+C
Sbjct: 48  FSRYCKLQTPCPLCSRLDHVLGNEEPEFYHNLLCGNHRSEVSSLISCQIHDKLADVHGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTDL 2
           E CLFS S   KSNSET+RLLVGKLG DL
Sbjct: 108 EECLFS-STIKKSNSETHRLLVGKLGLDL 135


>emb|CAN75727.1| hypothetical protein VITISV_031407 [Vitis vinifera]
          Length = 778

 Score =  129 bits (323), Expect = 6e-28
 Identities = 61/89 (68%), Positives = 73/89 (82%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           F+R+C+LQ PC LCSRLD+VLGNE+  FY +L+CG H+SE+SSL  C IHDKL +VHG+C
Sbjct: 48  FSRYCKLQTPCPLCSRLDHVLGNEEPEFYHNLLCGNHRSEVSSLISCQIHDKLADVHGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTDL 2
           E CLFS S   KSNSET+RLLVGKLG DL
Sbjct: 108 EECLFS-STIKKSNSETHRLLVGKLGLDL 135


>ref|XP_006423971.1| hypothetical protein CICLE_v10027718mg [Citrus clementina]
           gi|557525905|gb|ESR37211.1| hypothetical protein
           CICLE_v10027718mg [Citrus clementina]
          Length = 1099

 Score =  128 bits (321), Expect = 9e-28
 Identities = 59/88 (67%), Positives = 71/88 (80%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA +CELQ PCLLCSRLD+VLGNEK   Y DLICG H+ EISSL  CH H+KLV+V G+C
Sbjct: 48  FAHYCELQRPCLLCSRLDHVLGNEKLNPYRDLICGKHRLEISSLVLCHAHNKLVDVRGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           +NCLFSF+  ++S +ETY LLVGK+G D
Sbjct: 108 QNCLFSFAKVNRSITETYSLLVGKVGDD 135


>ref|XP_006443890.1| hypothetical protein CICLE_v10018827mg [Citrus clementina]
           gi|568851791|ref|XP_006479570.1| PREDICTED:
           uncharacterized protein LOC102624412 isoform X1 [Citrus
           sinensis] gi|568851793|ref|XP_006479571.1| PREDICTED:
           uncharacterized protein LOC102624412 isoform X2 [Citrus
           sinensis] gi|557546152|gb|ESR57130.1| hypothetical
           protein CICLE_v10018827mg [Citrus clementina]
          Length = 862

 Score =  125 bits (314), Expect = 6e-27
 Identities = 56/88 (63%), Positives = 68/88 (77%)
 Frame = -1

Query: 268 FARFCELQIPCLLCSRLDNVLGNEKSGFYWDLICGAHKSEISSLAFCHIHDKLVNVHGLC 89
           FA +CELQIPC+LCSRLD+VLGNEK  FY +L+C +H+SEISSL  CHIH KL   HG+C
Sbjct: 48  FAHYCELQIPCILCSRLDHVLGNEKPEFYRNLLCSSHRSEISSLISCHIHGKLAAAHGMC 107

Query: 88  ENCLFSFSMDHKSNSETYRLLVGKLGTD 5
           ++CL SFS    SN + +RL VGKLG D
Sbjct: 108 DDCLLSFSTKSNSNLDVHRLFVGKLGFD 135