BLASTX nr result
ID: Akebia25_contig00042615
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Akebia25_contig00042615
(374 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EME41417.1| hypothetical protein DOTSEDRAFT_156254 [Dothistro... 164 1e-38
gb|EPE24092.1| MFS general substrate transporter [Glarea lozoyen... 153 3e-35
gb|EME88661.1| hypothetical protein MYCFIDRAFT_80042 [Pseudocerc... 152 4e-35
ref|XP_001213347.1| conserved hypothetical protein [Aspergillus ... 147 1e-33
ref|XP_001263248.1| MFS sugar transporter, putative [Neosartorya... 146 3e-33
ref|XP_001210292.1| conserved hypothetical protein [Aspergillus ... 146 3e-33
ref|XP_754404.1| MFS sugar transporter [Aspergillus fumigatus Af... 145 4e-33
gb|EHK97473.1| putative High-affinity glucose transporter SNF3 [... 145 4e-33
gb|EDP52535.1| MFS sugar transporter, putative [Aspergillus fumi... 144 1e-32
ref|XP_001271213.1| MFS sugar transporter, putative [Aspergillus... 144 1e-32
ref|XP_662654.1| hypothetical protein AN5050.2 [Aspergillus nidu... 144 1e-32
ref|XP_002556740.1| Pc06g01340 [Penicillium chrysogenum Wisconsi... 143 2e-32
ref|XP_001935478.1| sugar carrier protein A [Pyrenophora tritici... 143 3e-32
ref|XP_003654906.1| hypothetical protein THITE_2118138 [Thielavi... 141 8e-32
ref|XP_007585134.1| putative mfs sugar transporter protein [Neof... 140 2e-31
ref|XP_003302613.1| hypothetical protein PTT_14491 [Pyrenophora ... 140 2e-31
gb|EHA23122.1| hypothetical protein ASPNIDRAFT_197549 [Aspergill... 138 9e-31
ref|XP_001399955.1| MFS sugar transporter [Aspergillus niger CBS... 136 3e-30
ref|XP_003665724.1| MFS sugar transporter-like protein [Myceliop... 135 4e-30
ref|XP_001589077.1| hypothetical protein SS1G_09710 [Sclerotinia... 134 1e-29
>gb|EME41417.1| hypothetical protein DOTSEDRAFT_156254 [Dothistroma septosporum
NZE10]
Length = 504
Score = 164 bits (415), Expect = 1e-38
Identities = 80/107 (74%), Positives = 90/107 (84%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
EIMPMQIRGRG AFATGIGNWLVST+FAQVSPIALG I WKYYFVFVA+NILVTIPTIFF
Sbjct: 389 EIMPMQIRGRGTAFATGIGNWLVSTVFAQVSPIALGSIKWKYYFVFVAWNILVTIPTIFF 448
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESVIRTGSIAGEK 323
F ETN++SLE+IDLLFG RALG L E+ +K +E +R S+A K
Sbjct: 449 FFRETNQLSLEDIDLLFGGRALGTLPEELGQKDVEEAVRRESVASGK 495
>gb|EPE24092.1| MFS general substrate transporter [Glarea lozoyensis ATCC 20868]
Length = 492
Score = 153 bits (386), Expect = 3e-35
Identities = 72/97 (74%), Positives = 85/97 (87%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIR RGNAFATGIGNWLV+TLFAQVSPI LGKIGWK+YFVFVAFNI+VT P IFF
Sbjct: 385 EVMPMQIRARGNAFATGIGNWLVATLFAQVSPIGLGKIGWKFYFVFVAFNIIVTFPVIFF 444
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESV 293
F ET ++SLE+IDLLFGERALG L ++++++ I V
Sbjct: 445 FFKETKQVSLEDIDLLFGERALGTLPDDLTKEGITEV 481
>gb|EME88661.1| hypothetical protein MYCFIDRAFT_80042 [Pseudocercospora fijiensis
CIRAD86]
Length = 520
Score = 152 bits (385), Expect = 4e-35
Identities = 76/121 (62%), Positives = 89/121 (73%), Gaps = 4/121 (3%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
EIMPMQIRGRGNAFATGIGNWL+ST QVSP+ALG IGWK+YF+FVAFN++VTIP I+
Sbjct: 389 EIMPMQIRGRGNAFATGIGNWLMSTFITQVSPVALGSIGWKFYFLFVAFNLVVTIPVIWV 448
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESVIRTGSIAG----EKEAATTRTED 350
F ETN +SLEEID+LFGERALG L EI E +E +R G EKE + +
Sbjct: 449 FFQETNGLSLEEIDILFGERALGTLPREIGEGDVEDAVRRRGSVGVDDKEKEVEVLQRDV 508
Query: 351 V 353
V
Sbjct: 509 V 509
>ref|XP_001213347.1| conserved hypothetical protein [Aspergillus terreus NIH2624]
gi|114194271|gb|EAU35971.1| conserved hypothetical
protein [Aspergillus terreus NIH2624]
Length = 492
Score = 147 bits (372), Expect = 1e-33
Identities = 70/96 (72%), Positives = 82/96 (85%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIRG GNAFATG+GNW VSTL+AQVSPIALG IGWK+ FVF AFN+LVT PTI+F
Sbjct: 385 EVMPMQIRGTGNAFATGVGNWAVSTLWAQVSPIALGSIGWKFNFVFAAFNLLVTFPTIYF 444
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIES 290
+F ET + SLEEIDLLFG RALG L +++EKQ E+
Sbjct: 445 LFVETKQKSLEEIDLLFGGRALGTLPEQVNEKQEET 480
>ref|XP_001263248.1| MFS sugar transporter, putative [Neosartorya fischeri NRRL 181]
gi|119411408|gb|EAW21351.1| MFS sugar transporter,
putative [Neosartorya fischeri NRRL 181]
Length = 491
Score = 146 bits (369), Expect = 3e-33
Identities = 69/103 (66%), Positives = 85/103 (82%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIRG+GNAFATGIGNW V+TL+AQVSPIALGK+GWK+YFVFVA+NI +T+P I+F
Sbjct: 385 EVMPMQIRGKGNAFATGIGNWAVATLWAQVSPIALGKLGWKFYFVFVAWNICITLPVIYF 444
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESVIRTGSI 311
F ET + SLEEIDLLFG RALG L ++EK E+ I ++
Sbjct: 445 CFKETKQKSLEEIDLLFGGRALGTLPENVAEKGEETGISVTNV 487
>ref|XP_001210292.1| conserved hypothetical protein [Aspergillus terreus NIH2624]
gi|114197152|gb|EAU38852.1| conserved hypothetical
protein [Aspergillus terreus NIH2624]
Length = 494
Score = 146 bits (368), Expect = 3e-33
Identities = 73/115 (63%), Positives = 89/115 (77%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIRG+G AFATGIGNW VSTL++QVSPIALGKIGWK+YF+F A+N++VTIPTIF
Sbjct: 385 EVMPMQIRGKGVAFATGIGNWTVSTLWSQVSPIALGKIGWKFYFIFAAWNVVVTIPTIFI 444
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESVIRTGSIAGEKEAATTRTE 347
F ET + SLEEIDLLFG RALG LD+E++ K ++ E AA T+ E
Sbjct: 445 WFKETKQKSLEEIDLLFGGRALGVLDDELNPKALDM---------ESTAAATQIE 490
>ref|XP_754404.1| MFS sugar transporter [Aspergillus fumigatus Af293]
gi|66852041|gb|EAL92366.1| MFS sugar transporter,
putative [Aspergillus fumigatus Af293]
Length = 594
Score = 145 bits (367), Expect = 4e-33
Identities = 69/103 (66%), Positives = 85/103 (82%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIRG+GNAFATGIGNW V+TL+AQVSPIALGK+GWK+YFVFVA+NI +T+P I+F
Sbjct: 488 EVMPMQIRGKGNAFATGIGNWAVATLWAQVSPIALGKLGWKFYFVFVAWNICITLPVIYF 547
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESVIRTGSI 311
F ET + SLEEIDLLFG RALG L ++EK E+ I ++
Sbjct: 548 CFKETKQKSLEEIDLLFGGRALGTLPENMAEKGEETGISVTNV 590
>gb|EHK97473.1| putative High-affinity glucose transporter SNF3 [Glarea lozoyensis
74030]
Length = 653
Score = 145 bits (367), Expect = 4e-33
Identities = 68/84 (80%), Positives = 77/84 (91%)
Frame = +3
Query: 6 IMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFFV 185
+MPMQIR RGNAFATGIGNWLV+TLFAQVSPI LGKIGWK+YFVFVAFNI+VT P IFF
Sbjct: 239 VMPMQIRARGNAFATGIGNWLVATLFAQVSPIGLGKIGWKFYFVFVAFNIIVTFPVIFFF 298
Query: 186 FPETNKMSLEEIDLLFGERALGAL 257
F ET ++SLE+IDLLFGERALG++
Sbjct: 299 FKETKQVSLEDIDLLFGERALGSV 322
>gb|EDP52535.1| MFS sugar transporter, putative [Aspergillus fumigatus A1163]
Length = 625
Score = 144 bits (364), Expect = 1e-32
Identities = 68/103 (66%), Positives = 85/103 (82%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIRG+GNAFATGIGNW V+TL+AQVSPIALGK+GWK+YFVFVA+NI +T+P I+F
Sbjct: 519 EVMPMQIRGKGNAFATGIGNWAVATLWAQVSPIALGKLGWKFYFVFVAWNICITLPVIYF 578
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESVIRTGSI 311
F ET + SLEEIDLLFG RALG L +++K E+ I ++
Sbjct: 579 CFKETKQKSLEEIDLLFGGRALGTLPENMADKGEETGISVTNV 621
>ref|XP_001271213.1| MFS sugar transporter, putative [Aspergillus clavatus NRRL 1]
gi|119399359|gb|EAW09787.1| MFS sugar transporter,
putative [Aspergillus clavatus NRRL 1]
Length = 491
Score = 144 bits (364), Expect = 1e-32
Identities = 67/103 (65%), Positives = 84/103 (81%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIRG+GNAFATGIGNW V+TL+AQVSPIALG IGWK+YF+F AFNI V++P I+F
Sbjct: 385 EVMPMQIRGKGNAFATGIGNWTVATLWAQVSPIALGSIGWKFYFIFAAFNICVSLPVIYF 444
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESVIRTGSI 311
+F ET + SLEEIDLLFG RALG L ++EK ++ I ++
Sbjct: 445 LFKETKQKSLEEIDLLFGGRALGTLPENVAEKSDDTGISVTNV 487
>ref|XP_662654.1| hypothetical protein AN5050.2 [Aspergillus nidulans FGSC A4]
gi|40741938|gb|EAA61128.1| hypothetical protein AN5050.2
[Aspergillus nidulans FGSC A4]
gi|259482071|tpe|CBF76199.1| TPA: MFS sugar transporter,
putative (AFU_orthologue; AFUA_3G12170) [Aspergillus
nidulans FGSC A4]
Length = 495
Score = 144 bits (364), Expect = 1e-32
Identities = 67/100 (67%), Positives = 82/100 (82%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIRGRGNAFATG+GNW VSTL++QVSPIALGKIGWK+YF+F +NI VT+P ++F
Sbjct: 388 EVMPMQIRGRGNAFATGVGNWTVSTLWSQVSPIALGKIGWKFYFIFAGWNICVTLPVVYF 447
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESVIRT 302
F ET + SLEEID+LFG RALG L +I++K E V T
Sbjct: 448 FFIETKQKSLEEIDILFGGRALGTLPEDIADKDNEVVATT 487
>ref|XP_002556740.1| Pc06g01340 [Penicillium chrysogenum Wisconsin 54-1255]
gi|211581353|emb|CAP79127.1| Pc06g01340 [Penicillium
chrysogenum Wisconsin 54-1255]
Length = 494
Score = 143 bits (361), Expect = 2e-32
Identities = 67/95 (70%), Positives = 81/95 (85%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIRG+G AFATG+GNW VSTL++QVSPIALGKIGWK+Y +F A+N+ VTIPTIFF
Sbjct: 385 EVMPMQIRGKGVAFATGVGNWTVSTLWSQVSPIALGKIGWKFYLIFAAWNVCVTIPTIFF 444
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIE 287
F ET + SLEEIDLLFG RALGAL++ + K +E
Sbjct: 445 WFKETKQKSLEEIDLLFGGRALGALNDNLDSKALE 479
>ref|XP_001935478.1| sugar carrier protein A [Pyrenophora tritici-repentis Pt-1C-BFP]
gi|187981426|gb|EDU48052.1| sugar carrier protein A
[Pyrenophora tritici-repentis Pt-1C-BFP]
Length = 504
Score = 143 bits (360), Expect = 3e-32
Identities = 69/114 (60%), Positives = 90/114 (78%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
EIMP+ IRGRG+AFATG+GNWLV T+++QVSPI LG I +K+YF+FVAFN+LVT+PT+FF
Sbjct: 388 EIMPLSIRGRGSAFATGVGNWLVGTVWSQVSPIGLGNITYKFYFIFVAFNLLVTLPTVFF 447
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESVIRTGSIAGEKEAATTRT 344
VF ET +++LEEIDLLFGERALG L + + +KQ + S A ++ A T T
Sbjct: 448 VFKETKQLTLEEIDLLFGERALGILPDNLDDKQKVIELERISAAKKQLAGTNVT 501
>ref|XP_003654906.1| hypothetical protein THITE_2118138 [Thielavia terrestris NRRL 8126]
gi|347002169|gb|AEO68570.1| hypothetical protein
THITE_2118138 [Thielavia terrestris NRRL 8126]
Length = 492
Score = 141 bits (356), Expect = 8e-32
Identities = 64/94 (68%), Positives = 84/94 (89%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIR RGNAFATGIGNWLVST ++QVSPIAL ++ WK+YF+FVA++I+VTIPT+FF
Sbjct: 387 EVMPMQIRARGNAFATGIGNWLVSTFWSQVSPIALKQLSWKFYFLFVAWDIVVTIPTVFF 446
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQI 284
VF ET ++SLEEIDLLFGER+LG L ++++ ++
Sbjct: 447 VFKETKQLSLEEIDLLFGERSLGTLPKDLNKGEL 480
>ref|XP_007585134.1| putative mfs sugar transporter protein [Neofusicoccum parvum
UCRNP2] gi|485921763|gb|EOD47407.1| putative mfs sugar
transporter protein [Neofusicoccum parvum UCRNP2]
Length = 492
Score = 140 bits (353), Expect = 2e-31
Identities = 66/95 (69%), Positives = 80/95 (84%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
EIMPMQIR RG+AFATGIGNWLVST+++QVSPI LG IGWK+YF+FVA+NI VT+P I
Sbjct: 385 EIMPMQIRARGSAFATGIGNWLVSTIWSQVSPIGLGAIGWKFYFIFVAWNICVTLPVIII 444
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIE 287
F ET K+SLEEIDLLFGERALG L + I + +++
Sbjct: 445 YFKETKKLSLEEIDLLFGERALGNLPDNIQDVKLD 479
>ref|XP_003302613.1| hypothetical protein PTT_14491 [Pyrenophora teres f. teres 0-1]
gi|311321946|gb|EFQ89314.1| hypothetical protein
PTT_14491 [Pyrenophora teres f. teres 0-1]
Length = 499
Score = 140 bits (353), Expect = 2e-31
Identities = 67/111 (60%), Positives = 89/111 (80%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
EIMP+ IRGRG+AFATG+GNWLV T+++QVSPI LG I +K+YF+FVAFN+LVT+PT+FF
Sbjct: 388 EIMPLSIRGRGSAFATGVGNWLVGTVWSQVSPIGLGNITYKFYFIFVAFNLLVTLPTVFF 447
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIESVIRTGSIAGEKEAAT 335
VF ET +++LEEID LFGERA G L +++ +KQ + S A ++ AAT
Sbjct: 448 VFKETKQLTLEEIDFLFGERAEGMLPDDLDDKQKVIELERISAAKKQLAAT 498
>gb|EHA23122.1| hypothetical protein ASPNIDRAFT_197549 [Aspergillus niger ATCC
1015]
Length = 480
Score = 138 bits (347), Expect = 9e-31
Identities = 64/92 (69%), Positives = 77/92 (83%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIRGRGNAFAT IGNW VSTL+ QVSPIAL KI WK+YFVF A+N+ +T+PT++F
Sbjct: 385 EVMPMQIRGRGNAFATAIGNWAVSTLWNQVSPIALDKIQWKFYFVFAAWNVCITLPTVYF 444
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEK 278
F ETN+ SLEEIDLLFG RALG L +++ K
Sbjct: 445 FFKETNQKSLEEIDLLFGGRALGMLPEDVAAK 476
>ref|XP_001399955.1| MFS sugar transporter [Aspergillus niger CBS 513.88]
gi|134056881|emb|CAK37785.1| unnamed protein product
[Aspergillus niger]
Length = 489
Score = 136 bits (343), Expect = 3e-30
Identities = 63/92 (68%), Positives = 76/92 (82%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIRGRGNAFAT IGNW VSTL+ QVSPI L KI WK+YFVF A+N+ +T+PT++F
Sbjct: 385 EVMPMQIRGRGNAFATAIGNWAVSTLWNQVSPIVLDKIQWKFYFVFAAWNVCITLPTVYF 444
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEK 278
F ETN+ SLEEIDLLFG RALG L +++ K
Sbjct: 445 FFKETNQKSLEEIDLLFGGRALGMLPEDVAAK 476
>ref|XP_003665724.1| MFS sugar transporter-like protein [Myceliophthora thermophila ATCC
42464] gi|347012996|gb|AEO60479.1| MFS sugar
transporter-like protein [Myceliophthora thermophila
ATCC 42464]
Length = 492
Score = 135 bits (341), Expect = 4e-30
Identities = 60/95 (63%), Positives = 82/95 (86%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIR RGNAFATG+GNWLVST ++Q+SP+AL K+ WK+YF+FVA+++LVTIP +FF
Sbjct: 387 EVMPMQIRARGNAFATGLGNWLVSTFWSQLSPMALKKLEWKFYFLFVAWDVLVTIPVVFF 446
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISEKQIE 287
F ET ++SLEEIDLLFGER+LG L ++++ ++
Sbjct: 447 FFKETKQLSLEEIDLLFGERSLGTLPKDLNKGTLD 481
>ref|XP_001589077.1| hypothetical protein SS1G_09710 [Sclerotinia sclerotiorum 1980]
gi|154694105|gb|EDN93843.1| hypothetical protein
SS1G_09710 [Sclerotinia sclerotiorum 1980 UF-70]
Length = 493
Score = 134 bits (338), Expect = 1e-29
Identities = 62/91 (68%), Positives = 77/91 (84%)
Frame = +3
Query: 3 EIMPMQIRGRGNAFATGIGNWLVSTLFAQVSPIALGKIGWKYYFVFVAFNILVTIPTIFF 182
E+MPMQIR RGNAF+TGIGNWLV+T +AQVSP AL + WK+YF+F A+N++VTIP +FF
Sbjct: 386 EVMPMQIRARGNAFSTGIGNWLVATFWAQVSPQALKHLTWKFYFLFAAWNVVVTIPVVFF 445
Query: 183 VFPETNKMSLEEIDLLFGERALGALDNEISE 275
F ET ++SLEEIDLLFGERALG L +IS+
Sbjct: 446 FFKETKQLSLEEIDLLFGERALGTLPEDISK 476