BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia25_contig00034070
         (260 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   129   3e-28
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc...   127   1e-27
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra...   107   1e-21
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe...   107   2e-21
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...    98   1e-18
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...    96   5e-18
gb|AAP45313.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...    94   2e-17
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...    92   1e-16
gb|ACO90238.1| norcoclaurine 6-O-methyltransferase [Thalictrum f...    92   1e-16
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]         89   6e-16
gb|AFB74613.1| O-methyltransferase 3 [Papaver somniferum] gi|388...    87   2e-15
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt...    87   3e-15
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth...    87   3e-15
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap...    86   4e-15
gb|ACO90225.1| putative norcoclaurine 6-O-methyltransferase [Pap...    79   5e-13
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ...    77   3e-12
gb|ACN88562.1| norreticuline-7-O-methyltransferase [Papaver somn...    73   4e-11
ref|XP_002278127.1| PREDICTED: isoflavone-7-O-methyltransferase ...    59   9e-07
emb|CAN75970.1| hypothetical protein VITISV_009881 [Vitis vinifera]    59   9e-07
ref|XP_004144872.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran...    55   8e-06

>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  129 bits (325), Expect = 3e-28
 Identities = 62/86 (72%), Positives = 73/86 (84%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAI 181
           LP+SNVN DNLYR+LRYL+ M ILR+EK DD  K YALEPIA  L R++++SMVP+IL +
Sbjct: 59  LPVSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGM 118

Query: 182 TQKDFVTPWHYMKDGLSDEGTAFEKA 259
           TQKDF+TPWH MKDGLSD GTAFEKA
Sbjct: 119 TQKDFMTPWHSMKDGLSDNGTAFEKA 144


>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
           4'-O-methyltransferase; AltName:
           Full=S-adenosyl-L-methionine:3'-hydroxy-N-
           methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
           gi|9711916|dbj|BAB08005.1|
           S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
           4'-O-methyltransferase [Coptis japonica]
          Length = 350

 Score =  127 bits (320), Expect = 1e-27
 Identities = 61/86 (70%), Positives = 73/86 (84%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAI 181
           LP+S+VN DNLYR+LRYL+ M ILR+EK DD  K YALEPIA  L R++++SMVP+IL +
Sbjct: 59  LPVSDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGM 118

Query: 182 TQKDFVTPWHYMKDGLSDEGTAFEKA 259
           TQKDF+TPWH MKDGLSD GTAFEKA
Sbjct: 119 TQKDFMTPWHSMKDGLSDNGTAFEKA 144


>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
           [Thalictrum flavum subsp. glaucum]
          Length = 348

 Score =  107 bits (268), Expect = 1e-21
 Identities = 54/87 (62%), Positives = 68/87 (78%), Gaps = 1/87 (1%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAI 181
           LP+SNVN DNL+R+LRYL+ M +L     D     YALEPIAK +LR+ ++SMVP++L +
Sbjct: 60  LPISNVNLDNLFRILRYLVKMELLSYAADDK----YALEPIAKLVLRNEKRSMVPMVLGM 115

Query: 182 TQKDFVTPWHYMKDGLSDEG-TAFEKA 259
           TQKDF+TPWH MKDGL+D G TAFEKA
Sbjct: 116 TQKDFMTPWHSMKDGLTDNGVTAFEKA 142


>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
           californica]
          Length = 353

 Score =  107 bits (266), Expect = 2e-21
 Identities = 50/89 (56%), Positives = 68/89 (76%), Gaps = 4/89 (4%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMD----DDIKGYALEPIAKFLLRDSEKSMVPI 169
           LP+SNVN DNLYRVLRYL+HM IL+  K      +  K Y+LEP+   L++D+E++MVPI
Sbjct: 58  LPISNVNSDNLYRVLRYLVHMGILKETKSTINGGEIKKLYSLEPVGSLLVKDAERNMVPI 117

Query: 170 ILAITQKDFVTPWHYMKDGLSDEGTAFEK 256
           +L +TQ+DF+ PWHY+K+GL +  TAFEK
Sbjct: 118 VLGMTQQDFMIPWHYIKEGLGEGSTAFEK 146


>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
           Full=S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase; Short=6-OMT
           gi|9711914|dbj|BAB08004.1|
           S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score = 98.2 bits (243), Expect = 1e-18
 Identities = 49/87 (56%), Positives = 64/87 (73%), Gaps = 1/87 (1%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAI 181
           LP   VN+D LYRV+RYL+HM++     +D +++ Y L P AK+L++  +K MV  ILAI
Sbjct: 55  LPSQPVNEDALYRVMRYLVHMKLFTKASIDGELR-YGLAPPAKYLVKGWDKCMVGSILAI 113

Query: 182 TQKDFVTPWHYMKDGLSDE-GTAFEKA 259
           T KDF+ PWHY+KDGLS E GTAFEKA
Sbjct: 114 TDKDFMAPWHYLKDGLSGESGTAFEKA 140


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase 2 [Papaver somniferum]
           gi|571330882|gb|AHF27397.1|
           S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'- O-methyltransferase 2 [synthetic construct]
          Length = 357

 Score = 95.9 bits (237), Expect = 5e-18
 Identities = 45/87 (51%), Positives = 66/87 (75%), Gaps = 2/87 (2%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDDI-KGYALEPIAKFLLRDSEKSMVPIILA 178
           LP++NV+ D LYR++RYL+H+ I+  E  +  + K Y+L+P+   LLRD+E+SMVP+IL 
Sbjct: 64  LPITNVSSDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILG 123

Query: 179 ITQKDFVTPWHYMKDGLSD-EGTAFEK 256
           +TQKDF+  WH+MK+GL +   TAFEK
Sbjct: 124 MTQKDFMVSWHFMKEGLGNGSTTAFEK 150


>gb|AAP45313.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase 1 [Papaver somniferum]
          Length = 354

 Score = 94.0 bits (232), Expect = 2e-17
 Identities = 46/88 (52%), Positives = 66/88 (75%), Gaps = 2/88 (2%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDDI-KGYALEPIAKFLLRDSEKSMVPIILA 178
           LPLSNVN DNLYR+LRYL+H+ IL  +     + + Y+L+P+   LL+DSE+SM P+IL 
Sbjct: 61  LPLSNVNSDNLYRLLRYLVHLNILGQQTCAAGVDRVYSLKPVGTLLLKDSERSMAPVILG 120

Query: 179 ITQKDFVTPWHYMKDGL-SDEGTAFEKA 259
           ++QKDF+  W+++K+GL +   TAFEKA
Sbjct: 121 LSQKDFLFVWNFVKEGLGTGSTTAFEKA 148


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
           glaucum]
          Length = 350

 Score = 91.7 bits (226), Expect = 1e-16
 Identities = 45/89 (50%), Positives = 62/89 (69%), Gaps = 3/89 (3%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAI 181
           LP   VN D LYRVLRYL+HM++     +D +++ Y L P AKFL++  +K M+  IL I
Sbjct: 56  LPSQPVNQDALYRVLRYLVHMKLFTKSSIDGELR-YGLAPPAKFLVKGWDKCMLGAILTI 114

Query: 182 TQKDFVTPWHYMKDGLSDEG---TAFEKA 259
           T KDF+ PWHY+K+G+ ++G   TAFEKA
Sbjct: 115 TDKDFMAPWHYLKEGILNDGSTSTAFEKA 143


>gb|ACO90238.1| norcoclaurine 6-O-methyltransferase [Thalictrum flavum]
          Length = 225

 Score = 91.7 bits (226), Expect = 1e-16
 Identities = 45/89 (50%), Positives = 62/89 (69%), Gaps = 3/89 (3%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAI 181
           LP   VN D LYRVLRYL+HM++     +D +++ Y L P AKFL++  +K M+  IL I
Sbjct: 56  LPSQPVNQDALYRVLRYLVHMKLFTKSSIDGELR-YGLAPPAKFLVKGWDKCMLGAILTI 114

Query: 182 TQKDFVTPWHYMKDGLSDEG---TAFEKA 259
           T KDF+ PWHY+K+G+ ++G   TAFEKA
Sbjct: 115 TDKDFMAPWHYLKEGILNDGSTSTAFEKA 143


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score = 89.0 bits (219), Expect = 6e-16
 Identities = 44/88 (50%), Positives = 65/88 (73%), Gaps = 2/88 (2%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDD--IKGYALEPIAKFLLRDSEKSMVPIIL 175
           LP+  V+ D LYRV+RYL+HM++ + ++ +D+  IK Y+L P AKFL++  EKSMV  IL
Sbjct: 58  LPVQPVDLDRLYRVMRYLVHMKLFKKQEDEDEESIK-YSLLPPAKFLIKGWEKSMVSAIL 116

Query: 176 AITQKDFVTPWHYMKDGLSDEGTAFEKA 259
            I  KDF++PWH++K+GL +   AF+KA
Sbjct: 117 LINDKDFMSPWHHLKEGLVNNSNAFDKA 144


>gb|AFB74613.1| O-methyltransferase 3 [Papaver somniferum]
           gi|388571230|gb|AFK73711.1| O-methyltransferase [Papaver
           somniferum]
          Length = 339

 Score = 87.4 bits (215), Expect = 2e-15
 Identities = 42/86 (48%), Positives = 58/86 (67%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAI 181
           LP   VN+D LYR+L +L+HM++      D   + Y+L P AK+LL+  EKSMVP IL++
Sbjct: 56  LPAQPVNEDRLYRILHFLVHMKLFN---KDATTQKYSLAPPAKYLLKGWEKSMVPSILSV 112

Query: 182 TQKDFVTPWHYMKDGLSDEGTAFEKA 259
           T KDF  PW+++ DGL+    AFEKA
Sbjct: 113 TDKDFTAPWNHLGDGLTGNCNAFEKA 138


>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase
           [Papaver somniferum]
          Length = 346

 Score = 86.7 bits (213), Expect = 3e-15
 Identities = 44/87 (50%), Positives = 60/87 (68%), Gaps = 1/87 (1%)
 Frame = +2

Query: 2   LPLSN-VNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILA 178
           LP++  VN+D L+R++RYL+HM +    K+D   + Y+L P AK+LLR  EKSMV  IL 
Sbjct: 56  LPVAQPVNEDRLFRIMRYLVHMELF---KIDATTQKYSLAPPAKYLLRGWEKSMVDSILC 112

Query: 179 ITQKDFVTPWHYMKDGLSDEGTAFEKA 259
           I  KDF+ PWH++ DGL+    AFEKA
Sbjct: 113 INDKDFLAPWHHLGDGLTGNCDAFEKA 139


>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; Short=6OMT
           gi|33286374|gb|AAQ01669.1| (R,S)-norcoclaurine
           6-O-methyltransferase [Papaver somniferum]
           gi|571330878|gb|AHF27395.1| (R,S)-norcoclaurine
           6-O-methyltransferase [synthetic construct]
          Length = 346

 Score = 86.7 bits (213), Expect = 3e-15
 Identities = 44/87 (50%), Positives = 60/87 (68%), Gaps = 1/87 (1%)
 Frame = +2

Query: 2   LPLSN-VNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILA 178
           LP++  VN+D L+R++RYL+HM +    K+D   + Y+L P AK+LLR  EKSMV  IL 
Sbjct: 56  LPVAQPVNEDRLFRIMRYLVHMELF---KIDATTQKYSLAPPAKYLLRGWEKSMVDSILC 112

Query: 179 ITQKDFVTPWHYMKDGLSDEGTAFEKA 259
           I  KDF+ PWH++ DGL+    AFEKA
Sbjct: 113 INDKDFLAPWHHLGDGLTGNCDAFEKA 139


>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
          Length = 346

 Score = 86.3 bits (212), Expect = 4e-15
 Identities = 42/81 (51%), Positives = 56/81 (69%)
 Frame = +2

Query: 17  VNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAITQKDF 196
           VN+D L+R++RYL+HM +    K+D   + Y+L P AK+LLR  EKSMV  IL I  KDF
Sbjct: 62  VNEDRLFRIMRYLVHMELF---KIDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDF 118

Query: 197 VTPWHYMKDGLSDEGTAFEKA 259
           + PWH++ DGL+    AFEKA
Sbjct: 119 LAPWHHLGDGLTGNCDAFEKA 139


>gb|ACO90225.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
          Length = 357

 Score = 79.3 bits (194), Expect = 5e-13
 Identities = 41/95 (43%), Positives = 60/95 (63%), Gaps = 10/95 (10%)
 Frame = +2

Query: 2   LPLSN-VNDDNLYRVLRYLIHMRILRLEKMDDDIKG---------YALEPIAKFLLRDSE 151
           LP+   VN D LYRV+R+L+HM++   E +   + G         Y+L P AK+L+R SE
Sbjct: 56  LPIDQPVNIDRLYRVMRFLVHMKLFNKENISI-LSGETAQVVEDKYSLAPPAKYLIRGSE 114

Query: 152 KSMVPIILAITQKDFVTPWHYMKDGLSDEGTAFEK 256
           +SMVP +L IT +D V PWH++K+G++     FEK
Sbjct: 115 QSMVPWVLGITDEDLVAPWHWLKEGITGNCNVFEK 149


>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum
           flavum]
          Length = 346

 Score = 76.6 bits (187), Expect = 3e-12
 Identities = 38/89 (42%), Positives = 58/89 (65%), Gaps = 3/89 (3%)
 Frame = +2

Query: 2   LPLSNVNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAI 181
           LP   VN D LYRV+RYL+H+++     +D +++ Y L P AKFL++  +  M+  IL +
Sbjct: 51  LPSQPVNQDALYRVMRYLVHIKLFTKSSIDGELR-YGLAPPAKFLVKGWDNCMIGSILLM 109

Query: 182 TQKDFVTPWHYMKDGLSDEG---TAFEKA 259
              DF+ P+HY+KD + ++G   TAFEKA
Sbjct: 110 VDTDFMAPYHYIKDEILNDGSSITAFEKA 138


>gb|ACN88562.1| norreticuline-7-O-methyltransferase [Papaver somniferum]
          Length = 357

 Score = 73.2 bits (178), Expect = 4e-11
 Identities = 40/94 (42%), Positives = 54/94 (57%), Gaps = 8/94 (8%)
 Frame = +2

Query: 2   LPLSN-VNDDNLYRVLRYLIHMRILRLEKMDDDIKG-------YALEPIAKFLLRDSEKS 157
           LP+   VN D LYRV+RYL+H ++   E +     G       Y L P AK+L+R S++S
Sbjct: 56  LPIDQPVNIDRLYRVMRYLVHQKLFNKEVISTLNGGTVQVTEKYWLAPPAKYLIRGSQQS 115

Query: 158 MVPIILAITQKDFVTPWHYMKDGLSDEGTAFEKA 259
           MVP +L I  +D   PWH +KD L+ E   FE A
Sbjct: 116 MVPSVLGIIDEDMFAPWHILKDSLTGECNIFETA 149


>ref|XP_002278127.1| PREDICTED: isoflavone-7-O-methyltransferase 9 [Vitis vinifera]
          Length = 354

 Score = 58.5 bits (140), Expect = 9e-07
 Identities = 26/77 (33%), Positives = 47/77 (61%), Gaps = 1/77 (1%)
 Frame = +2

Query: 32  LYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAITQKDFVTPWH 211
           L++++R L+H     ++K+D++ +GY L P ++ L++ +  S+ PI+L +     VTPWH
Sbjct: 70  LHQLMRLLVHSGFFAMQKVDENQEGYVLTPPSRLLVKGNATSLAPIVLGMLDPVLVTPWH 129

Query: 212 YMKDGLSDEG-TAFEKA 259
           ++   L     TAFE A
Sbjct: 130 FLGSWLQGSSLTAFEAA 146


>emb|CAN75970.1| hypothetical protein VITISV_009881 [Vitis vinifera]
          Length = 354

 Score = 58.5 bits (140), Expect = 9e-07
 Identities = 26/77 (33%), Positives = 47/77 (61%), Gaps = 1/77 (1%)
 Frame = +2

Query: 32  LYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAITQKDFVTPWH 211
           L++++R L+H     ++K+D++ +GY L P ++ L++ +  S+ PI+L +     VTPWH
Sbjct: 70  LHQLMRLLVHSGFFAMQKVDENQEGYVLTPPSRLLVKGNATSLAPIVLGMLDPVLVTPWH 129

Query: 212 YMKDGLSDEG-TAFEKA 259
           ++   L     TAFE A
Sbjct: 130 FLGSWLQGSSLTAFEAA 146


>ref|XP_004144872.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis
           sativus] gi|449470501|ref|XP_004152955.1| PREDICTED:
           (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis
           sativus] gi|449526577|ref|XP_004170290.1| PREDICTED:
           (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis
           sativus]
          Length = 357

 Score = 55.5 bits (132), Expect = 8e-06
 Identities = 25/77 (32%), Positives = 44/77 (57%)
 Frame = +2

Query: 14  NVNDDNLYRVLRYLIHMRILRLEKMDDDIKGYALEPIAKFLLRDSEKSMVPIILAITQKD 193
           N +   L+R+LR+LIH  I + E +D++  GY+  P+++ L  + E SM P++L  T   
Sbjct: 64  NCSSSLLHRILRFLIHRGIFKQETIDENQIGYSQTPMSRLLATNVENSMAPLLLLETSPV 123

Query: 194 FVTPWHYMKDGLSDEGT 244
            + PW ++   L +  T
Sbjct: 124 MLAPWQHLSGHLKNSDT 140