BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia25_contig00033184
         (296 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_007213760.1| hypothetical protein PRUPE_ppa003763m2g, par...   135   5e-30
ref|XP_004294193.1| PREDICTED: methyltransferase-like protein 25...   133   3e-29
ref|XP_007022295.1| S-adenosyl-L-methionine-dependent methyltran...   132   4e-29
ref|XP_007022294.1| S-adenosyl-L-methionine-dependent methyltran...   132   4e-29
ref|XP_006441027.1| hypothetical protein CICLE_v10021587mg [Citr...   132   7e-29
ref|XP_006477996.1| PREDICTED: protein RRNAD1-like [Citrus sinen...   129   3e-28
ref|XP_006416795.1| hypothetical protein EUTSA_v10007372mg [Eutr...   129   6e-28
ref|XP_002890179.1| hypothetical protein ARALYDRAFT_889058 [Arab...   126   3e-27
ref|XP_004243992.1| PREDICTED: methyltransferase-like protein 25...   125   5e-27
ref|NP_173110.2| S-adenosyl-L-methionine-dependent methyltransfe...   125   8e-27
ref|XP_006307588.1| hypothetical protein CARUB_v10009211mg [Caps...   124   1e-26
ref|XP_004963148.1| PREDICTED: methyltransferase-like protein 25...   124   2e-26
ref|XP_004963147.1| PREDICTED: methyltransferase-like protein 25...   124   2e-26
ref|NP_001067247.1| Os12g0610500 [Oryza sativa Japonica Group] g...   123   3e-26
gb|EEE53589.1| hypothetical protein OsJ_36831 [Oryza sativa Japo...   123   3e-26
ref|XP_002529955.1| protein with unknown function [Ricinus commu...   122   4e-26
ref|XP_002310689.1| hypothetical protein POPTR_0007s08440g [Popu...   122   4e-26
ref|XP_006346052.1| PREDICTED: protein RRNAD1-like isoform X2 [S...   122   5e-26
ref|XP_006346051.1| PREDICTED: protein RRNAD1-like isoform X1 [S...   122   5e-26
ref|XP_006836403.1| hypothetical protein AMTR_s00092p00143460 [A...   122   5e-26

>ref|XP_007213760.1| hypothetical protein PRUPE_ppa003763m2g, partial [Prunus persica]
           gi|462409625|gb|EMJ14959.1| hypothetical protein
           PRUPE_ppa003763m2g, partial [Prunus persica]
          Length = 265

 Score =  135 bits (341), Expect = 5e-30
 Identities = 64/97 (65%), Positives = 86/97 (88%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           +IDKEWM+CLR+E+VENLL+IP G+VQD+WPASLK+FI TL SLVLP+ +++LQ +LP +
Sbjct: 50  AIDKEWMDCLRKEAVENLLQIPGGVVQDHWPASLKKFIVTLSSLVLPRQQQDLQTVLPGM 109

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
           ++ SL+SVL  GMN KKKHEVE+L+A+V+SIA +VGA
Sbjct: 110 NMTSLNSVLSTGMNWKKKHEVEVLSAVVDSIATSVGA 146


>ref|XP_004294193.1| PREDICTED: methyltransferase-like protein 25-like [Fragaria vesca
           subsp. vesca]
          Length = 548

 Score =  133 bits (334), Expect = 3e-29
 Identities = 62/97 (63%), Positives = 82/97 (84%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           +ID +WM+CLR+E VENLL+IPSG+VQD+WPASLKEF+ TLRSL LP+ + +LQ +LP +
Sbjct: 46  AIDHDWMDCLRQEPVENLLQIPSGVVQDHWPASLKEFVVTLRSLALPRQQADLQAVLPGI 105

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
            + SL+SVL  GMN KKKHEVEIL+A+V+S+A  +GA
Sbjct: 106 SMTSLNSVLSTGMNSKKKHEVEILSAVVSSVANNIGA 142


>ref|XP_007022295.1| S-adenosyl-L-methionine-dependent methyltransferases superfamily
           protein, putative isoform 2 [Theobroma cacao]
           gi|508721923|gb|EOY13820.1|
           S-adenosyl-L-methionine-dependent methyltransferases
           superfamily protein, putative isoform 2 [Theobroma
           cacao]
          Length = 427

 Score =  132 bits (333), Expect = 4e-29
 Identities = 62/97 (63%), Positives = 81/97 (83%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           ++DKEW+ECL +E+VENLL IPSG+VQD+WP SLK+FI TL+SLV P+ + +L K+ P  
Sbjct: 48  AVDKEWIECLSKEAVENLLLIPSGVVQDHWPTSLKDFILTLKSLVFPREQADLGKVFPGF 107

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
           H  SL+SVL QGMNLKKKHEVE+L+A+V+SIA T+ A
Sbjct: 108 HTTSLTSVLAQGMNLKKKHEVEVLSAIVSSIASTMTA 144


>ref|XP_007022294.1| S-adenosyl-L-methionine-dependent methyltransferases superfamily
           protein, putative isoform 1 [Theobroma cacao]
           gi|508721922|gb|EOY13819.1|
           S-adenosyl-L-methionine-dependent methyltransferases
           superfamily protein, putative isoform 1 [Theobroma
           cacao]
          Length = 539

 Score =  132 bits (333), Expect = 4e-29
 Identities = 62/97 (63%), Positives = 81/97 (83%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           ++DKEW+ECL +E+VENLL IPSG+VQD+WP SLK+FI TL+SLV P+ + +L K+ P  
Sbjct: 48  AVDKEWIECLSKEAVENLLLIPSGVVQDHWPTSLKDFILTLKSLVFPREQADLGKVFPGF 107

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
           H  SL+SVL QGMNLKKKHEVE+L+A+V+SIA T+ A
Sbjct: 108 HTTSLTSVLAQGMNLKKKHEVEVLSAIVSSIASTMTA 144


>ref|XP_006441027.1| hypothetical protein CICLE_v10021587mg [Citrus clementina]
           gi|557543289|gb|ESR54267.1| hypothetical protein
           CICLE_v10021587mg [Citrus clementina]
          Length = 278

 Score =  132 bits (331), Expect = 7e-29
 Identities = 63/97 (64%), Positives = 80/97 (82%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           ++DK+W++CLR ES ENLL IPSGIVQD+WP SLKEF+ TL+SL  P+ + +LQ +LP  
Sbjct: 48  AVDKDWIDCLRNESTENLLLIPSGIVQDHWPDSLKEFVLTLKSLEFPREQADLQMVLPSF 107

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
            V SL++VL QGMNLKKKHEVE+L+A+VNSIA TV A
Sbjct: 108 PVTSLNTVLAQGMNLKKKHEVEVLSAVVNSIANTVKA 144


>ref|XP_006477996.1| PREDICTED: protein RRNAD1-like [Citrus sinensis]
          Length = 560

 Score =  129 bits (325), Expect = 3e-28
 Identities = 62/97 (63%), Positives = 79/97 (81%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           ++DK+W++CLR ES ENLL IPSGIVQD+WP SLKEF+ TL+SL  P+ + +LQ + P  
Sbjct: 48  AVDKDWIDCLRNESTENLLLIPSGIVQDHWPDSLKEFVLTLKSLEFPRQQADLQMVWPSF 107

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
            V SL++VL QGMNLKKKHEVE+L+A+VNSIA TV A
Sbjct: 108 PVTSLNTVLAQGMNLKKKHEVEVLSAVVNSIANTVKA 144


>ref|XP_006416795.1| hypothetical protein EUTSA_v10007372mg [Eutrema salsugineum]
           gi|557094566|gb|ESQ35148.1| hypothetical protein
           EUTSA_v10007372mg [Eutrema salsugineum]
          Length = 520

 Score =  129 bits (323), Expect = 6e-28
 Identities = 61/96 (63%), Positives = 78/96 (81%)
 Frame = -1

Query: 293 IDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDLH 114
           +D+EWM CLR E  EN+L IPSG VQD+WPASLKEF+HTLRSL  P+ + ELQ IL D+ 
Sbjct: 48  VDQEWMSCLRNEKPENILLIPSGGVQDHWPASLKEFVHTLRSLSFPREQAELQAILADVD 107

Query: 113 VPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
           +  LS+VL QGMNLKKKHEV++L+A+V+S+  +VGA
Sbjct: 108 MAPLSTVLSQGMNLKKKHEVQVLSAVVSSVINSVGA 143


>ref|XP_002890179.1| hypothetical protein ARALYDRAFT_889058 [Arabidopsis lyrata subsp.
           lyrata] gi|297336021|gb|EFH66438.1| hypothetical protein
           ARALYDRAFT_889058 [Arabidopsis lyrata subsp. lyrata]
          Length = 520

 Score =  126 bits (317), Expect = 3e-27
 Identities = 57/97 (58%), Positives = 80/97 (82%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           ++D+ WM CL++E  EN+L IPSG+VQD+WPASLKEF+HTLRSL  P+ + +L  IL D+
Sbjct: 47  AVDEVWMSCLKDEKPENILLIPSGVVQDHWPASLKEFVHTLRSLSFPREQADLHAILADV 106

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
            +  LS+VL QGMNLKKKHEVE+L+++V+S+ ++VGA
Sbjct: 107 DLVPLSTVLSQGMNLKKKHEVEVLSSVVSSVVKSVGA 143


>ref|XP_004243992.1| PREDICTED: methyltransferase-like protein 25-like [Solanum
           lycopersicum]
          Length = 540

 Score =  125 bits (315), Expect = 5e-27
 Identities = 60/98 (61%), Positives = 82/98 (83%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           ++DKEWM+CL++ESVENLL+IPSG+VQD+WP SLK+FI TLRSL L + + + Q+    +
Sbjct: 48  AVDKEWMDCLQKESVENLLRIPSGVVQDHWPGSLKDFILTLRSLCLSREQADFQE-FSGM 106

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGAQ 3
           H+ SLS+VL QGMN KKKHEVE+L+ALV+ +A+ VGA+
Sbjct: 107 HIASLSNVLAQGMNHKKKHEVEVLSALVSLVAKQVGAR 144


>ref|NP_173110.2| S-adenosyl-L-methionine-dependent methyltransferases superfamily
           protein [Arabidopsis thaliana]
           gi|190016010|gb|ACE62893.1| At1g16650 [Arabidopsis
           thaliana] gi|332191359|gb|AEE29480.1| uncharacterized
           protein AT1G16650 [Arabidopsis thaliana]
          Length = 521

 Score =  125 bits (313), Expect = 8e-27
 Identities = 57/97 (58%), Positives = 81/97 (83%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           ++++EWM CL++E  EN+L IPSG VQD+WPASLK+F+HTLRSL  P+ + +LQ IL D+
Sbjct: 47  AVNEEWMSCLKDEKPENILLIPSGGVQDHWPASLKKFVHTLRSLSFPREQADLQAILADV 106

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
            +  LS+VL QGMNLKKKHEVE+L+++V+S+ ++VGA
Sbjct: 107 DMVPLSTVLSQGMNLKKKHEVEVLSSVVSSVVKSVGA 143


>ref|XP_006307588.1| hypothetical protein CARUB_v10009211mg [Capsella rubella]
           gi|482576299|gb|EOA40486.1| hypothetical protein
           CARUB_v10009211mg [Capsella rubella]
          Length = 428

 Score =  124 bits (311), Expect = 1e-26
 Identities = 58/97 (59%), Positives = 78/97 (80%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           ++D++WM CL++E  EN+L IPSG VQD+WPASLKEFIHTLRSL  P+ +   Q IL D+
Sbjct: 47  AVDEQWMSCLKDEKPENVLLIPSGGVQDHWPASLKEFIHTLRSLSFPREQANFQAILADV 106

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
            +  LS+VL QGMNLKKKHEVE+L+++V+S+  +VGA
Sbjct: 107 DMVPLSTVLSQGMNLKKKHEVEVLSSVVSSVVNSVGA 143


>ref|XP_004963148.1| PREDICTED: methyltransferase-like protein 25-like isoform X2
           [Setaria italica]
          Length = 530

 Score =  124 bits (310), Expect = 2e-26
 Identities = 56/97 (57%), Positives = 80/97 (82%)
 Frame = -1

Query: 293 IDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDLH 114
           +D EWMECLR E V +LLK+PSG VQ++WPA+L+EF+ T RSLV+P+ ++  Q ++PD+H
Sbjct: 31  VDAEWMECLRREPVGSLLKLPSGCVQEHWPATLREFVLTARSLVVPREQKAPQSLVPDIH 90

Query: 113 VPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGAQ 3
           V S+ +VL QGMN KKKHE+E LAA+V++IA++ GA+
Sbjct: 91  VASIGTVLAQGMNSKKKHEIENLAAVVHAIAKSCGAK 127


>ref|XP_004963147.1| PREDICTED: methyltransferase-like protein 25-like isoform X1
           [Setaria italica]
          Length = 550

 Score =  124 bits (310), Expect = 2e-26
 Identities = 56/97 (57%), Positives = 80/97 (82%)
 Frame = -1

Query: 293 IDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDLH 114
           +D EWMECLR E V +LLK+PSG VQ++WPA+L+EF+ T RSLV+P+ ++  Q ++PD+H
Sbjct: 55  VDAEWMECLRREPVGSLLKLPSGCVQEHWPATLREFVLTARSLVVPREQKAPQSLVPDIH 114

Query: 113 VPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGAQ 3
           V S+ +VL QGMN KKKHE+E LAA+V++IA++ GA+
Sbjct: 115 VASIGTVLAQGMNSKKKHEIENLAAVVHAIAKSCGAK 151


>ref|NP_001067247.1| Os12g0610500 [Oryza sativa Japonica Group]
           gi|77556570|gb|ABA99366.1| expressed protein [Oryza
           sativa Japonica Group] gi|113649754|dbj|BAF30266.1|
           Os12g0610500 [Oryza sativa Japonica Group]
          Length = 576

 Score =  123 bits (308), Expect = 3e-26
 Identities = 55/97 (56%), Positives = 79/97 (81%)
 Frame = -1

Query: 293 IDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDLH 114
           +D EWMECLR E VE+LL +PSG VQ++WP+SL++F+ T +SLVLP+ ++  + +LPDLH
Sbjct: 57  VDAEWMECLRREPVESLLMLPSGCVQEHWPSSLRDFVLTAKSLVLPREQKSPRSLLPDLH 116

Query: 113 VPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGAQ 3
           V S+++VL QGMN KKKHE+E LA LV++I ++ GA+
Sbjct: 117 VASINTVLAQGMNSKKKHEIETLAGLVHAITKSCGAK 153


>gb|EEE53589.1| hypothetical protein OsJ_36831 [Oryza sativa Japonica Group]
          Length = 669

 Score =  123 bits (308), Expect = 3e-26
 Identities = 55/97 (56%), Positives = 79/97 (81%)
 Frame = -1

Query: 293 IDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDLH 114
           +D EWMECLR E VE+LL +PSG VQ++WP+SL++F+ T +SLVLP+ ++  + +LPDLH
Sbjct: 57  VDAEWMECLRREPVESLLMLPSGCVQEHWPSSLRDFVLTAKSLVLPREQKSPRSLLPDLH 116

Query: 113 VPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGAQ 3
           V S+++VL QGMN KKKHE+E LA LV++I ++ GA+
Sbjct: 117 VASINTVLAQGMNSKKKHEIETLAGLVHAITKSCGAK 153


>ref|XP_002529955.1| protein with unknown function [Ricinus communis]
           gi|223530553|gb|EEF32432.1| protein with unknown
           function [Ricinus communis]
          Length = 538

 Score =  122 bits (307), Expect = 4e-26
 Identities = 55/98 (56%), Positives = 81/98 (82%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           SID++W+ CLR E ++NLL +PSG+ QD+WP SLKEF+ ++RSL+  + + +L K+LP L
Sbjct: 44  SIDQQWIHCLRHEPLQNLLLLPSGVFQDHWPPSLKEFVMSMRSLMFSREQVDLAKVLPGL 103

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGAQ 3
           ++ SL++VL QGMN+KKKHEVE+L+A+V+SIA +V AQ
Sbjct: 104 NITSLNTVLSQGMNMKKKHEVEVLSAVVSSIANSVSAQ 141


>ref|XP_002310689.1| hypothetical protein POPTR_0007s08440g [Populus trichocarpa]
           gi|222853592|gb|EEE91139.1| hypothetical protein
           POPTR_0007s08440g [Populus trichocarpa]
          Length = 548

 Score =  122 bits (307), Expect = 4e-26
 Identities = 57/98 (58%), Positives = 77/98 (78%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           SID  W+ CLR E V+NLL IP GI+Q++WPASLKEF+  L+SL  P+ +  L K+ P +
Sbjct: 49  SIDPVWLHCLRNEPVQNLLLIPCGIIQEHWPASLKEFVLDLKSLAFPREQAHLNKLFPGV 108

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGAQ 3
           +V SL++VL QGMN KKKHEVE+L+A+V+S+A +VGAQ
Sbjct: 109 NVVSLNTVLAQGMNFKKKHEVEVLSAVVSSVANSVGAQ 146


>ref|XP_006346052.1| PREDICTED: protein RRNAD1-like isoform X2 [Solanum tuberosum]
          Length = 500

 Score =  122 bits (306), Expect = 5e-26
 Identities = 60/97 (61%), Positives = 79/97 (81%)
 Frame = -1

Query: 293 IDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDLH 114
           +DKEWM+CLR+ESV NLL+IPSG+VQD+WP SLKEFI TLRSL L + + + Q+    +H
Sbjct: 49  VDKEWMDCLRKESVGNLLRIPSGVVQDHWPGSLKEFILTLRSLSLSREQADFQE-FSGMH 107

Query: 113 VPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGAQ 3
           + SL++VL QGMN KKKHEVE L+ALV+ +A+ VGA+
Sbjct: 108 IASLNNVLAQGMNHKKKHEVEALSALVSLVAKQVGAR 144


>ref|XP_006346051.1| PREDICTED: protein RRNAD1-like isoform X1 [Solanum tuberosum]
          Length = 541

 Score =  122 bits (306), Expect = 5e-26
 Identities = 60/97 (61%), Positives = 79/97 (81%)
 Frame = -1

Query: 293 IDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDLH 114
           +DKEWM+CLR+ESV NLL+IPSG+VQD+WP SLKEFI TLRSL L + + + Q+    +H
Sbjct: 49  VDKEWMDCLRKESVGNLLRIPSGVVQDHWPGSLKEFILTLRSLSLSREQADFQE-FSGMH 107

Query: 113 VPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGAQ 3
           + SL++VL QGMN KKKHEVE L+ALV+ +A+ VGA+
Sbjct: 108 IASLNNVLAQGMNHKKKHEVEALSALVSLVAKQVGAR 144


>ref|XP_006836403.1| hypothetical protein AMTR_s00092p00143460 [Amborella trichopoda]
           gi|548838921|gb|ERM99256.1| hypothetical protein
           AMTR_s00092p00143460 [Amborella trichopoda]
          Length = 577

 Score =  122 bits (306), Expect = 5e-26
 Identities = 59/97 (60%), Positives = 74/97 (76%)
 Frame = -1

Query: 296 SIDKEWMECLREESVENLLKIPSGIVQDYWPASLKEFIHTLRSLVLPQGKEELQKILPDL 117
           ++D++W++CLR+E V NLL IPSG VQD WP SLKEFI+T RSL  P+  E L+ I P +
Sbjct: 47  TLDQQWLDCLRDEPVRNLLSIPSGTVQDNWPCSLKEFINTARSLSFPKEGESLEMIFPGV 106

Query: 116 HVPSLSSVLIQGMNLKKKHEVEILAALVNSIARTVGA 6
            V  +S+VL QGMNLKKKHEVEILAA V  +A +VGA
Sbjct: 107 RVEPVSNVLAQGMNLKKKHEVEILAATVGRVASSVGA 143