BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia25_contig00028896
         (234 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   112   4e-23
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc...   110   2e-22
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe...    94   2e-17
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra...    93   3e-17
gb|AAP45313.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...    87   3e-15
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...    85   9e-15
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]         79   7e-13
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...    77   3e-12
gb|ACO90225.1| putative norcoclaurine 6-O-methyltransferase [Pap...    77   3e-12
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt...    75   1e-11
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth...    75   1e-11
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...    74   2e-11
gb|ACO90238.1| norcoclaurine 6-O-methyltransferase [Thalictrum f...    74   2e-11
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap...    74   2e-11
gb|AFB74613.1| O-methyltransferase 3 [Papaver somniferum] gi|388...    74   2e-11
gb|ACN88562.1| norreticuline-7-O-methyltransferase [Papaver somn...    72   1e-10
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ...    60   4e-07

>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  112 bits (281), Expect = 4e-23
 Identities = 52/77 (67%), Positives = 64/77 (83%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILGI 54
           LP+SNVNCDNLYR+LRYL+ M ILR+EK DD    YALEPIA  L R++++SMVP+ILG+
Sbjct: 59  LPVSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGM 118

Query: 53  SQKDCVKPWHYMKDGLS 3
           +QKD + PWH MKDGLS
Sbjct: 119 TQKDFMTPWHSMKDGLS 135


>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
           4'-O-methyltransferase; AltName:
           Full=S-adenosyl-L-methionine:3'-hydroxy-N-
           methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
           gi|9711916|dbj|BAB08005.1|
           S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
           4'-O-methyltransferase [Coptis japonica]
          Length = 350

 Score =  110 bits (276), Expect = 2e-22
 Identities = 51/77 (66%), Positives = 64/77 (83%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILGI 54
           LP+S+VNCDNLYR+LRYL+ M ILR+EK DD    YALEPIA  L R++++SMVP+ILG+
Sbjct: 59  LPVSDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGM 118

Query: 53  SQKDCVKPWHYMKDGLS 3
           +QKD + PWH MKDGLS
Sbjct: 119 TQKDFMTPWHSMKDGLS 135


>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
           californica]
          Length = 353

 Score = 94.0 bits (232), Expect = 2e-17
 Identities = 45/82 (54%), Positives = 62/82 (75%), Gaps = 6/82 (7%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNG------YALEPIAKFLLRDSEKSMV 72
           LP+SNVN DNLYRVLRYL+HM IL+  K     NG      Y+LEP+   L++D+E++MV
Sbjct: 58  LPISNVNSDNLYRVLRYLVHMGILKETK--STINGGEIKKLYSLEPVGSLLVKDAERNMV 115

Query: 71  PIILGISQKDCVKPWHYMKDGL 6
           PI+LG++Q+D + PWHY+K+GL
Sbjct: 116 PIVLGMTQQDFMIPWHYIKEGL 137


>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
           [Thalictrum flavum subsp. glaucum]
          Length = 348

 Score = 93.2 bits (230), Expect = 3e-17
 Identities = 44/77 (57%), Positives = 59/77 (76%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILGI 54
           LP+SNVN DNL+R+LRYL+ M +L     D     YALEPIAK +LR+ ++SMVP++LG+
Sbjct: 60  LPISNVNLDNLFRILRYLVKMELLSYAADDK----YALEPIAKLVLRNEKRSMVPMVLGM 115

Query: 53  SQKDCVKPWHYMKDGLS 3
           +QKD + PWH MKDGL+
Sbjct: 116 TQKDFMTPWHSMKDGLT 132


>gb|AAP45313.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase 1 [Papaver somniferum]
          Length = 354

 Score = 86.7 bits (213), Expect = 3e-15
 Identities = 41/77 (53%), Positives = 58/77 (75%), Gaps = 1/77 (1%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNG-YALEPIAKFLLRDSEKSMVPIILG 57
           LPLSNVN DNLYR+LRYL+H+ IL  +      +  Y+L+P+   LL+DSE+SM P+ILG
Sbjct: 61  LPLSNVNSDNLYRLLRYLVHLNILGQQTCAAGVDRVYSLKPVGTLLLKDSERSMAPVILG 120

Query: 56  ISQKDCVKPWHYMKDGL 6
           +SQKD +  W+++K+GL
Sbjct: 121 LSQKDFLFVWNFVKEGL 137


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'-O-methyltransferase 2 [Papaver somniferum]
           gi|571330882|gb|AHF27397.1|
           S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
           4'- O-methyltransferase 2 [synthetic construct]
          Length = 357

 Score = 85.1 bits (209), Expect = 9e-15
 Identities = 38/77 (49%), Positives = 58/77 (75%), Gaps = 1/77 (1%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNG-YALEPIAKFLLRDSEKSMVPIILG 57
           LP++NV+ D LYR++RYL+H+ I+  E  +      Y+L+P+   LLRD+E+SMVP+ILG
Sbjct: 64  LPITNVSSDYLYRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILG 123

Query: 56  ISQKDCVKPWHYMKDGL 6
           ++QKD +  WH+MK+GL
Sbjct: 124 MTQKDFMVSWHFMKEGL 140


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score = 79.0 bits (193), Expect = 7e-13
 Identities = 37/77 (48%), Positives = 57/77 (74%), Gaps = 1/77 (1%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTN-GYALEPIAKFLLRDSEKSMVPIILG 57
           LP+  V+ D LYRV+RYL+HM++ + ++ +D+ +  Y+L P AKFL++  EKSMV  IL 
Sbjct: 58  LPVQPVDLDRLYRVMRYLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILL 117

Query: 56  ISQKDCVKPWHYMKDGL 6
           I+ KD + PWH++K+GL
Sbjct: 118 INDKDFMSPWHHLKEGL 134


>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
           Full=S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase; Short=6-OMT
           gi|9711914|dbj|BAB08004.1|
           S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score = 77.0 bits (188), Expect = 3e-12
 Identities = 38/77 (49%), Positives = 51/77 (66%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILGI 54
           LP   VN D LYRV+RYL+HM++     +D +   Y L P AK+L++  +K MV  IL I
Sbjct: 55  LPSQPVNEDALYRVMRYLVHMKLFTKASIDGELR-YGLAPPAKYLVKGWDKCMVGSILAI 113

Query: 53  SQKDCVKPWHYMKDGLS 3
           + KD + PWHY+KDGLS
Sbjct: 114 TDKDFMAPWHYLKDGLS 130


>gb|ACO90225.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
          Length = 357

 Score = 76.6 bits (187), Expect = 3e-12
 Identities = 38/86 (44%), Positives = 59/86 (68%), Gaps = 9/86 (10%)
 Frame = -2

Query: 233 LPLSN-VNCDNLYRVLRYLIHMRILRLEK---MDDDT-----NGYALEPIAKFLLRDSEK 81
           LP+   VN D LYRV+R+L+HM++   E    +  +T     + Y+L P AK+L+R SE+
Sbjct: 56  LPIDQPVNIDRLYRVMRFLVHMKLFNKENISILSGETAQVVEDKYSLAPPAKYLIRGSEQ 115

Query: 80  SMVPIILGISQKDCVKPWHYMKDGLS 3
           SMVP +LGI+ +D V PWH++K+G++
Sbjct: 116 SMVPWVLGITDEDLVAPWHWLKEGIT 141


>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase
           [Papaver somniferum]
          Length = 346

 Score = 74.7 bits (182), Expect = 1e-11
 Identities = 39/78 (50%), Positives = 54/78 (69%), Gaps = 1/78 (1%)
 Frame = -2

Query: 233 LPLSN-VNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILG 57
           LP++  VN D L+R++RYL+HM +    K+D  T  Y+L P AK+LLR  EKSMV  IL 
Sbjct: 56  LPVAQPVNEDRLFRIMRYLVHMELF---KIDATTQKYSLAPPAKYLLRGWEKSMVDSILC 112

Query: 56  ISQKDCVKPWHYMKDGLS 3
           I+ KD + PWH++ DGL+
Sbjct: 113 INDKDFLAPWHHLGDGLT 130


>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; Short=6OMT
           gi|33286374|gb|AAQ01669.1| (R,S)-norcoclaurine
           6-O-methyltransferase [Papaver somniferum]
           gi|571330878|gb|AHF27395.1| (R,S)-norcoclaurine
           6-O-methyltransferase [synthetic construct]
          Length = 346

 Score = 74.7 bits (182), Expect = 1e-11
 Identities = 39/78 (50%), Positives = 54/78 (69%), Gaps = 1/78 (1%)
 Frame = -2

Query: 233 LPLSN-VNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILG 57
           LP++  VN D L+R++RYL+HM +    K+D  T  Y+L P AK+LLR  EKSMV  IL 
Sbjct: 56  LPVAQPVNEDRLFRIMRYLVHMELF---KIDATTQKYSLAPPAKYLLRGWEKSMVDSILC 112

Query: 56  ISQKDCVKPWHYMKDGLS 3
           I+ KD + PWH++ DGL+
Sbjct: 113 INDKDFLAPWHHLGDGLT 130


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
           glaucum]
          Length = 350

 Score = 74.3 bits (181), Expect = 2e-11
 Identities = 36/76 (47%), Positives = 50/76 (65%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILGI 54
           LP   VN D LYRVLRYL+HM++     +D +   Y L P AKFL++  +K M+  IL I
Sbjct: 56  LPSQPVNQDALYRVLRYLVHMKLFTKSSIDGELR-YGLAPPAKFLVKGWDKCMLGAILTI 114

Query: 53  SQKDCVKPWHYMKDGL 6
           + KD + PWHY+K+G+
Sbjct: 115 TDKDFMAPWHYLKEGI 130


>gb|ACO90238.1| norcoclaurine 6-O-methyltransferase [Thalictrum flavum]
          Length = 225

 Score = 74.3 bits (181), Expect = 2e-11
 Identities = 36/76 (47%), Positives = 50/76 (65%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILGI 54
           LP   VN D LYRVLRYL+HM++     +D +   Y L P AKFL++  +K M+  IL I
Sbjct: 56  LPSQPVNQDALYRVLRYLVHMKLFTKSSIDGELR-YGLAPPAKFLVKGWDKCMLGAILTI 114

Query: 53  SQKDCVKPWHYMKDGL 6
           + KD + PWHY+K+G+
Sbjct: 115 TDKDFMAPWHYLKEGI 130


>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
          Length = 346

 Score = 74.3 bits (181), Expect = 2e-11
 Identities = 37/72 (51%), Positives = 50/72 (69%)
 Frame = -2

Query: 218 VNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILGISQKDC 39
           VN D L+R++RYL+HM +    K+D  T  Y+L P AK+LLR  EKSMV  IL I+ KD 
Sbjct: 62  VNEDRLFRIMRYLVHMELF---KIDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDF 118

Query: 38  VKPWHYMKDGLS 3
           + PWH++ DGL+
Sbjct: 119 LAPWHHLGDGLT 130


>gb|AFB74613.1| O-methyltransferase 3 [Papaver somniferum]
           gi|388571230|gb|AFK73711.1| O-methyltransferase [Papaver
           somniferum]
          Length = 339

 Score = 73.9 bits (180), Expect = 2e-11
 Identities = 36/77 (46%), Positives = 50/77 (64%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILGI 54
           LP   VN D LYR+L +L+HM++      D  T  Y+L P AK+LL+  EKSMVP IL +
Sbjct: 56  LPAQPVNEDRLYRILHFLVHMKLFN---KDATTQKYSLAPPAKYLLKGWEKSMVPSILSV 112

Query: 53  SQKDCVKPWHYMKDGLS 3
           + KD   PW+++ DGL+
Sbjct: 113 TDKDFTAPWNHLGDGLT 129


>gb|ACN88562.1| norreticuline-7-O-methyltransferase [Papaver somniferum]
          Length = 357

 Score = 71.6 bits (174), Expect = 1e-10
 Identities = 37/85 (43%), Positives = 51/85 (60%), Gaps = 8/85 (9%)
 Frame = -2

Query: 233 LPLSN-VNCDNLYRVLRYLIHMRILRLEKMDDDTNG-------YALEPIAKFLLRDSEKS 78
           LP+   VN D LYRV+RYL+H ++   E +     G       Y L P AK+L+R S++S
Sbjct: 56  LPIDQPVNIDRLYRVMRYLVHQKLFNKEVISTLNGGTVQVTEKYWLAPPAKYLIRGSQQS 115

Query: 77  MVPIILGISQKDCVKPWHYMKDGLS 3
           MVP +LGI  +D   PWH +KD L+
Sbjct: 116 MVPSVLGIIDEDMFAPWHILKDSLT 140


>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum
           flavum]
          Length = 346

 Score = 59.7 bits (143), Expect = 4e-07
 Identities = 30/74 (40%), Positives = 45/74 (60%)
 Frame = -2

Query: 233 LPLSNVNCDNLYRVLRYLIHMRILRLEKMDDDTNGYALEPIAKFLLRDSEKSMVPIILGI 54
           LP   VN D LYRV+RYL+H+++     +D +   Y L P AKFL++  +  M+  IL +
Sbjct: 51  LPSQPVNQDALYRVMRYLVHIKLFTKSSIDGELR-YGLAPPAKFLVKGWDNCMIGSILLM 109

Query: 53  SQKDCVKPWHYMKD 12
              D + P+HY+KD
Sbjct: 110 VDTDFMAPYHYIKD 123