BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia25_contig00004611
         (1241 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|EXC35400.1| hypothetical protein L484_026725 [Morus notabilis]     405   e-110
ref|XP_002272351.1| PREDICTED: transmembrane and coiled-coil dom...   403   e-110
ref|XP_007226229.1| hypothetical protein PRUPE_ppa016746mg [Prun...   401   e-109
ref|XP_004164664.1| PREDICTED: transmembrane and coiled-coil dom...   397   e-108
ref|XP_004146487.1| PREDICTED: transmembrane and coiled-coil dom...   397   e-108
ref|XP_006340700.1| PREDICTED: transmembrane and coiled-coil dom...   396   e-107
ref|XP_002310840.1| hypothetical protein POPTR_0007s13750g [Popu...   395   e-107
ref|XP_004233731.1| PREDICTED: uncharacterized membrane protein ...   394   e-107
ref|XP_007156109.1| hypothetical protein PHAVU_003G259400g [Phas...   393   e-106
ref|XP_007156108.1| hypothetical protein PHAVU_003G259400g [Phas...   393   e-106
ref|XP_004234544.1| PREDICTED: uncharacterized protein LOC101248...   392   e-106
ref|XP_007204629.1| hypothetical protein PRUPE_ppa002119mg [Prun...   391   e-106
ref|XP_006466894.1| PREDICTED: transmembrane and coiled-coil dom...   391   e-106
ref|XP_006425555.1| hypothetical protein CICLE_v10025097mg [Citr...   391   e-106
ref|NP_001078500.2| uncharacterized protein [Arabidopsis thalian...   390   e-106
ref|NP_849505.2| uncharacterized protein [Arabidopsis thaliana] ...   390   e-106
ref|XP_006343380.1| PREDICTED: transmembrane and coiled-coil dom...   390   e-106
ref|XP_006343379.1| PREDICTED: transmembrane and coiled-coil dom...   390   e-106
ref|XP_006343378.1| PREDICTED: transmembrane and coiled-coil dom...   390   e-106
ref|XP_002869040.1| hypothetical protein ARALYDRAFT_912740 [Arab...   390   e-106

>gb|EXC35400.1| hypothetical protein L484_026725 [Morus notabilis]
          Length = 663

 Score =  405 bits (1040), Expect = e-110
 Identities = 201/257 (78%), Positives = 223/257 (86%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV I+VSG VF+ EDFV+PWEGQ+DNLERYALQWESKNLIAVSTAIQDWLT
Sbjct: 401  GENHNQGRLAVEIMVSGFVFDKEDFVRPWEGQDDNLERYALQWESKNLIAVSTAIQDWLT 460

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SR+++ELMK+GAM+TVLS                DFIDSKWTIAVDRSDKAG+LLAEVL 
Sbjct: 461  SRIAMELMKQGAMLTVLSTLLAALAWPVTLLAATDFIDSKWTIAVDRSDKAGRLLAEVLL 520

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KG  GNRPVTL+G+SLGAR IFKCLQ LAE E+NA LVERVVLLGAP+SIK E W  ARK
Sbjct: 521  KGFHGNRPVTLVGYSLGARVIFKCLQFLAETENNAELVERVVLLGAPISIKDEKWGAARK 580

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRFVNAYST+DWTLG+AFRASLL++GLAGI PIDV GIENVDVT+L+EGHSSYLWTT
Sbjct: 581  MVAGRFVNAYSTNDWTLGVAFRASLLTQGLAGIQPIDVPGIENVDVTDLIEGHSSYLWTT 640

Query: 722  RLILELLELDTYYPVFR 772
            + I+ELLELD YYPVFR
Sbjct: 641  QRIIELLELDAYYPVFR 657


>ref|XP_002272351.1| PREDICTED: transmembrane and coiled-coil domain-containing protein 4
            [Vitis vinifera] gi|297735050|emb|CBI17412.3| unnamed
            protein product [Vitis vinifera]
          Length = 662

 Score =  403 bits (1035), Expect = e-110
 Identities = 200/264 (75%), Positives = 222/264 (84%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV ILVSG VF+ EDF+ PWEGQ +NLERYALQWESKNLIAVSTAIQDWLT
Sbjct: 398  GENHNQGRLAVEILVSGFVFQEEDFISPWEGQNNNLERYALQWESKNLIAVSTAIQDWLT 457

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            S ++L +MK+GAM+TVLS                DFIDSKWTIA+DRSDKAGKLLAEVL 
Sbjct: 458  SSIALSMMKQGAMLTVLSTLMTALAWPATLLAATDFIDSKWTIALDRSDKAGKLLAEVLQ 517

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRPVTL+G+SLGAR IFKCLQ LAE E NA LVERVVLLGAP+S + ENWE ARK
Sbjct: 518  KGLQGNRPVTLVGYSLGARVIFKCLQYLAETEQNAELVERVVLLGAPISFRDENWEVARK 577

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRFVNA+ST+DWTLG+AFRA+LL+KGLAGI P+DV GIENVDVTEL+EGHSSYLW T
Sbjct: 578  MVAGRFVNAFSTNDWTLGVAFRANLLTKGLAGIQPVDVPGIENVDVTELIEGHSSYLWVT 637

Query: 722  RLILELLELDTYYPVFRSFPAKPQ 793
              IL+ LELDTYYPVFR  P + Q
Sbjct: 638  EDILDRLELDTYYPVFRGSPTEHQ 661


>ref|XP_007226229.1| hypothetical protein PRUPE_ppa016746mg [Prunus persica]
            gi|462423165|gb|EMJ27428.1| hypothetical protein
            PRUPE_ppa016746mg [Prunus persica]
          Length = 671

 Score =  401 bits (1031), Expect = e-109
 Identities = 204/267 (76%), Positives = 227/267 (85%), Gaps = 1/267 (0%)
 Frame = +2

Query: 8    NHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLTSR 187
            N++QGRLAVGILVSG+VFE EDF++PWEGQ +NLERYAL WESKNLIA+STAIQDWLTSR
Sbjct: 403  NYDQGRLAVGILVSGLVFEDEDFIRPWEGQNENLERYALWWESKNLIALSTAIQDWLTSR 462

Query: 188  LSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLSKG 367
            ++LELMK GAMMTVL+                D IDSKW +AVDRSDKAGK+LAEVL KG
Sbjct: 463  IALELMKEGAMMTVLNTLLAAFAMPATLVTASDLIDSKWAVAVDRSDKAGKMLAEVLLKG 522

Query: 368  LQGNRPVTLIGFSLGARAIFKCLQCLAEME-DNAGLVERVVLLGAPVSIKGENWETARKM 544
            LQGNRPVTLIGFSLGAR +FKCLQCLAE E DNAGLVERVVLLGAP+SI+ ENW  ARKM
Sbjct: 523  LQGNRPVTLIGFSLGARVVFKCLQCLAETEGDNAGLVERVVLLGAPISIEEENWTDARKM 582

Query: 545  VAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTTR 724
            VAGRFVNAYS SDWTLGIAFRASLLS+GL GI P+DV GIENVDVT+LVEGHSSYLW T+
Sbjct: 583  VAGRFVNAYSISDWTLGIAFRASLLSQGLGGIQPVDVPGIENVDVTQLVEGHSSYLWKTK 642

Query: 725  LILELLELDTYYPVFRSFPAKPQDEKS 805
             ILE LELD+YYPVFR+   KPQ++ S
Sbjct: 643  QILEQLELDSYYPVFRT-TLKPQEDNS 668


>ref|XP_004164664.1| PREDICTED: transmembrane and coiled-coil domain-containing protein
            4-like [Cucumis sativus]
          Length = 673

 Score =  397 bits (1021), Expect = e-108
 Identities = 198/258 (76%), Positives = 220/258 (85%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV I VSG  F+ +DFV+PWEG  DNLERYALQWESKN+IAVSTAIQDWLT
Sbjct: 411  GENHNQGRLAVEITVSGFAFDDKDFVRPWEGLTDNLERYALQWESKNIIAVSTAIQDWLT 470

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SRL++ELMKRGAMMTVLS                DFIDSKWTIAVDRSDKAG++LA+VL 
Sbjct: 471  SRLAMELMKRGAMMTVLSSLLVALAWPATLLAATDFIDSKWTIAVDRSDKAGRILADVLL 530

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRPVTL+G+SLGAR IFKCLQ LAE E NA LVE+VVLLGAP+SIK +NWE ARK
Sbjct: 531  KGLQGNRPVTLVGYSLGARVIFKCLQYLAETEKNAELVEKVVLLGAPISIKDQNWEAARK 590

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRFVN YST+DW LGIAFRASLL++GLAGI PID+ GIENVDVT+++EGHSSYLW T
Sbjct: 591  MVAGRFVNVYSTNDWMLGIAFRASLLTQGLAGIQPIDIPGIENVDVTDVIEGHSSYLWAT 650

Query: 722  RLILELLELDTYYPVFRS 775
            R ILE LEL+TYYPVFRS
Sbjct: 651  RQILEQLELETYYPVFRS 668


>ref|XP_004146487.1| PREDICTED: transmembrane and coiled-coil domain-containing protein
            4-like [Cucumis sativus]
          Length = 673

 Score =  397 bits (1021), Expect = e-108
 Identities = 198/258 (76%), Positives = 220/258 (85%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV I VSG  F+ +DFV+PWEG  DNLERYALQWESKN+IAVSTAIQDWLT
Sbjct: 411  GENHNQGRLAVEITVSGFAFDDKDFVRPWEGLTDNLERYALQWESKNIIAVSTAIQDWLT 470

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SRL++ELMKRGAMMTVLS                DFIDSKWTIAVDRSDKAG++LA+VL 
Sbjct: 471  SRLAMELMKRGAMMTVLSSLLVALAWPATLLAATDFIDSKWTIAVDRSDKAGRILADVLL 530

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRPVTL+G+SLGAR IFKCLQ LAE E NA LVE+VVLLGAP+SIK +NWE ARK
Sbjct: 531  KGLQGNRPVTLVGYSLGARVIFKCLQYLAETEKNAELVEKVVLLGAPISIKDQNWEAARK 590

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRFVN YST+DW LGIAFRASLL++GLAGI PID+ GIENVDVT+++EGHSSYLW T
Sbjct: 591  MVAGRFVNVYSTNDWMLGIAFRASLLTQGLAGIQPIDIPGIENVDVTDVIEGHSSYLWAT 650

Query: 722  RLILELLELDTYYPVFRS 775
            R ILE LEL+TYYPVFRS
Sbjct: 651  RQILEQLELETYYPVFRS 668


>ref|XP_006340700.1| PREDICTED: transmembrane and coiled-coil domain-containing protein
            4-like [Solanum tuberosum]
          Length = 679

 Score =  396 bits (1017), Expect = e-107
 Identities = 195/258 (75%), Positives = 219/258 (84%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV +L+SG+VF+ EDFV+PWEGQ DN ERY LQWESKNLIAVSTAIQDWLT
Sbjct: 418  GENHNQGRLAVEVLISGLVFKEEDFVRPWEGQHDNSERYVLQWESKNLIAVSTAIQDWLT 477

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SRL++ELMKRGAMMTV+                 DFIDSKW IAVDRSDKAGKLLAEVL 
Sbjct: 478  SRLAMELMKRGAMMTVIKTLLTALALPATLLAMTDFIDSKWAIAVDRSDKAGKLLAEVLQ 537

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRPVTL+GFSLGAR IFKCLQ L+E E+ +GLVERVVLLGAP++IK  NWE ARK
Sbjct: 538  KGLQGNRPVTLVGFSLGARVIFKCLQVLSESENTSGLVERVVLLGAPIAIKNMNWEAARK 597

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            +VAGRFVNAY+T+DW LGIAFRASLL++GLAGI P+DV GIENVDVTEL++GHSSYLW T
Sbjct: 598  VVAGRFVNAYATNDWMLGIAFRASLLTRGLAGIQPVDVPGIENVDVTELIDGHSSYLWAT 657

Query: 722  RLILELLELDTYYPVFRS 775
            + IL+LLELD YYPV  S
Sbjct: 658  QKILDLLELDAYYPVVLS 675


>ref|XP_002310840.1| hypothetical protein POPTR_0007s13750g [Populus trichocarpa]
            gi|222853743|gb|EEE91290.1| hypothetical protein
            POPTR_0007s13750g [Populus trichocarpa]
          Length = 681

 Score =  395 bits (1014), Expect = e-107
 Identities = 196/264 (74%), Positives = 222/264 (84%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            G+NHNQGRLAVGI+VSG VF+ EDF+KPWEGQ DNLERYAL WE KNLIAVSTAIQDWLT
Sbjct: 416  GDNHNQGRLAVGIMVSGCVFDEEDFIKPWEGQNDNLERYALHWEPKNLIAVSTAIQDWLT 475

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SR+++E M++GAM+TVLS                DFIDSKWTIA+DRSDKAGKLLAEVL 
Sbjct: 476  SRIAMEFMRQGAMLTVLSTLLVALAWPATLLAATDFIDSKWTIAIDRSDKAGKLLAEVLL 535

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRPVTL+G+SLGAR IFKCL+ LAE E +A +VERVVLLGAP+SIK + WE ARK
Sbjct: 536  KGLQGNRPVTLVGYSLGARVIFKCLETLAETEHSAEVVERVVLLGAPISIKDQKWEAARK 595

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRFVNAYST DWTLG+AFRASLL++GLAGI P+DV GIENVDVT+LV+GHSSYLW T
Sbjct: 596  MVAGRFVNAYSTGDWTLGVAFRASLLTQGLAGIQPVDVPGIENVDVTDLVDGHSSYLWAT 655

Query: 722  RLILELLELDTYYPVFRSFPAKPQ 793
            + ILE LELD YY VFRS P  P+
Sbjct: 656  QQILEQLELDAYYHVFRS-PNPPE 678


>ref|XP_004233731.1| PREDICTED: uncharacterized membrane protein C6F6.13c-like [Solanum
            lycopersicum]
          Length = 659

 Score =  394 bits (1012), Expect = e-107
 Identities = 194/255 (76%), Positives = 217/255 (85%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV +L+SG+VF+ EDFV+PWEGQ DN ERY LQWESKNLIAVSTAIQDWLT
Sbjct: 398  GENHNQGRLAVEVLISGLVFKEEDFVRPWEGQHDNSERYVLQWESKNLIAVSTAIQDWLT 457

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SRL++ELMKRGAMMTVL                 DFIDSKW IAVDRSDKAGKLLAEVL 
Sbjct: 458  SRLAMELMKRGAMMTVLKTLLTALALPATLLAMTDFIDSKWAIAVDRSDKAGKLLAEVLQ 517

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRPVTL+GFSLGAR IFKCLQ LAE  + +GLVERVVLLGAP++IK  NWE ARK
Sbjct: 518  KGLQGNRPVTLVGFSLGARVIFKCLQVLAESANTSGLVERVVLLGAPIAIKSMNWEAARK 577

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            +VAGRFVNAY+T+DW LGIAFRASLL++GLAGI P+DV GIENVDVTEL++GHSSYLW T
Sbjct: 578  VVAGRFVNAYTTNDWMLGIAFRASLLTRGLAGIQPVDVPGIENVDVTELIDGHSSYLWAT 637

Query: 722  RLILELLELDTYYPV 766
            + IL+LL+LD YYPV
Sbjct: 638  QKILDLLDLDAYYPV 652


>ref|XP_007156109.1| hypothetical protein PHAVU_003G259400g [Phaseolus vulgaris]
            gi|561029463|gb|ESW28103.1| hypothetical protein
            PHAVU_003G259400g [Phaseolus vulgaris]
          Length = 668

 Score =  393 bits (1009), Expect = e-106
 Identities = 195/258 (75%), Positives = 218/258 (84%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRL V ILVSG VFE EDF++PWEG  DNLERYALQWESKNLIAVSTAIQDWLT
Sbjct: 406  GENHNQGRLGVEILVSGFVFEKEDFIRPWEGCNDNLERYALQWESKNLIAVSTAIQDWLT 465

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SRL++ELMKRGAMMTVLS                DFIDSKWTIA+ RS+KAGKLLAEVL 
Sbjct: 466  SRLAMELMKRGAMMTVLSSLLTALAWPAALLAATDFIDSKWTIAISRSNKAGKLLAEVLL 525

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRPVTLIG+SLGAR IFKCLQCLA+ E++A LVERVVLLGAP+ IK ENWE ARK
Sbjct: 526  KGLQGNRPVTLIGYSLGARVIFKCLQCLAKTENSAELVERVVLLGAPIPIKDENWEAARK 585

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRFVNAYS +DW LG+AFRASLL+KGLAGI P+D+ GI+NVDVT+ +EGHSSYLW T
Sbjct: 586  MVAGRFVNAYSRNDWMLGLAFRASLLTKGLAGIQPVDIPGIQNVDVTDHIEGHSSYLWAT 645

Query: 722  RLILELLELDTYYPVFRS 775
              IL+ L+LDTYYPV+ +
Sbjct: 646  PRILDQLQLDTYYPVYNN 663


>ref|XP_007156108.1| hypothetical protein PHAVU_003G259400g [Phaseolus vulgaris]
            gi|561029462|gb|ESW28102.1| hypothetical protein
            PHAVU_003G259400g [Phaseolus vulgaris]
          Length = 528

 Score =  393 bits (1009), Expect = e-106
 Identities = 195/258 (75%), Positives = 218/258 (84%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRL V ILVSG VFE EDF++PWEG  DNLERYALQWESKNLIAVSTAIQDWLT
Sbjct: 266  GENHNQGRLGVEILVSGFVFEKEDFIRPWEGCNDNLERYALQWESKNLIAVSTAIQDWLT 325

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SRL++ELMKRGAMMTVLS                DFIDSKWTIA+ RS+KAGKLLAEVL 
Sbjct: 326  SRLAMELMKRGAMMTVLSSLLTALAWPAALLAATDFIDSKWTIAISRSNKAGKLLAEVLL 385

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRPVTLIG+SLGAR IFKCLQCLA+ E++A LVERVVLLGAP+ IK ENWE ARK
Sbjct: 386  KGLQGNRPVTLIGYSLGARVIFKCLQCLAKTENSAELVERVVLLGAPIPIKDENWEAARK 445

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRFVNAYS +DW LG+AFRASLL+KGLAGI P+D+ GI+NVDVT+ +EGHSSYLW T
Sbjct: 446  MVAGRFVNAYSRNDWMLGLAFRASLLTKGLAGIQPVDIPGIQNVDVTDHIEGHSSYLWAT 505

Query: 722  RLILELLELDTYYPVFRS 775
              IL+ L+LDTYYPV+ +
Sbjct: 506  PRILDQLQLDTYYPVYNN 523


>ref|XP_004234544.1| PREDICTED: uncharacterized protein LOC101248750 [Solanum
            lycopersicum]
          Length = 661

 Score =  392 bits (1007), Expect = e-106
 Identities = 192/256 (75%), Positives = 219/256 (85%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV IL+SG+VFE EDF++PWEGQ DN ERY LQWESKNLIAVSTAIQDWLT
Sbjct: 399  GENHNQGRLAVQILISGLVFEEEDFIRPWEGQHDNSERYILQWESKNLIAVSTAIQDWLT 458

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SR+++ELMK+GAMMTVL                 DFIDS W+IAVDRSDKAG LLAEVL 
Sbjct: 459  SRIAMELMKQGAMMTVLHTLLTALAWPAALLTITDFIDSTWSIAVDRSDKAGVLLAEVLK 518

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQG+RPVTL+GFSLGAR IFKCLQ LAE E+N+GLVERVVLLGAP++IK  +WE ARK
Sbjct: 519  KGLQGHRPVTLVGFSLGARVIFKCLQVLAESENNSGLVERVVLLGAPIAIKDMDWEAARK 578

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            +VAGRFVNAYST+DW LG+AFRASLL++GLAGI P+++ G+ENVDVTE +EGHSSYLW T
Sbjct: 579  VVAGRFVNAYSTNDWMLGVAFRASLLTQGLAGIQPVEIPGVENVDVTEFIEGHSSYLWAT 638

Query: 722  RLILELLELDTYYPVF 769
            + ILELLELDTYYPVF
Sbjct: 639  QEILELLELDTYYPVF 654


>ref|XP_007204629.1| hypothetical protein PRUPE_ppa002119mg [Prunus persica]
            gi|462400160|gb|EMJ05828.1| hypothetical protein
            PRUPE_ppa002119mg [Prunus persica]
          Length = 713

 Score =  391 bits (1005), Expect = e-106
 Identities = 196/258 (75%), Positives = 216/258 (83%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV ILVSG VF+ EDFV+PWEGQ+DNLERYAL WESKNLIAVSTAIQDWLT
Sbjct: 451  GENHNQGRLAVEILVSGFVFDKEDFVRPWEGQDDNLERYALVWESKNLIAVSTAIQDWLT 510

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SR+++ LMK+GAMMTVL                 DFIDSKW IAVDRSDKAG+LLAEVL 
Sbjct: 511  SRIAMGLMKQGAMMTVLGTLVTALAWPATLLAAADFIDSKWAIAVDRSDKAGRLLAEVLL 570

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KG+ GNRPVTL+GFSLGAR IFKCLQCLAE E NA LVERVVLLGAP+SI  E WE ARK
Sbjct: 571  KGIHGNRPVTLVGFSLGARVIFKCLQCLAETEKNAELVERVVLLGAPISIADEKWEAARK 630

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRFVNAYST+DW LGIAFRASLLS+GLAGI PI V GIENVDVT++++ HSSYLW  
Sbjct: 631  MVAGRFVNAYSTNDWMLGIAFRASLLSRGLAGIQPIKVEGIENVDVTDVIDAHSSYLWGA 690

Query: 722  RLILELLELDTYYPVFRS 775
            + IL+LLEL+ YYPVFRS
Sbjct: 691  QQILDLLELEAYYPVFRS 708


>ref|XP_006466894.1| PREDICTED: transmembrane and coiled-coil domain-containing protein
            4-like isoform X1 [Citrus sinensis]
            gi|568825036|ref|XP_006466895.1| PREDICTED: transmembrane
            and coiled-coil domain-containing protein 4-like isoform
            X2 [Citrus sinensis] gi|568825038|ref|XP_006466896.1|
            PREDICTED: transmembrane and coiled-coil
            domain-containing protein 4-like isoform X3 [Citrus
            sinensis] gi|568825040|ref|XP_006466897.1| PREDICTED:
            transmembrane and coiled-coil domain-containing protein
            4-like isoform X4 [Citrus sinensis]
            gi|568825042|ref|XP_006466898.1| PREDICTED: transmembrane
            and coiled-coil domain-containing protein 4-like isoform
            X5 [Citrus sinensis]
          Length = 662

 Score =  391 bits (1004), Expect = e-106
 Identities = 195/260 (75%), Positives = 218/260 (83%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GEN NQGRLAV IL+SGVVF+ EDFV+PWEGQ DN+ERY LQWESKNLIAVSTAIQDWLT
Sbjct: 403  GENQNQGRLAVEILISGVVFDQEDFVRPWEGQNDNMERYVLQWESKNLIAVSTAIQDWLT 462

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SR+++ELMK+GAMMTVL                 D IDSKWTIAVDRSDKAGKLLAEVL 
Sbjct: 463  SRIAMELMKQGAMMTVLKTLLAALAWPATLVFAADLIDSKWTIAVDRSDKAGKLLAEVLM 522

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            +GLQG RPVTLIG+SLGAR IFKCL+ LAE E NAG+VERVVLLGAP+SIK +NWE  RK
Sbjct: 523  QGLQGYRPVTLIGYSLGARVIFKCLENLAENECNAGIVERVVLLGAPISIKDQNWEAVRK 582

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRF+N Y+T+DWTL IAFRASLLS+GLAGI PI+  GIEN+DVT L+EGHSSYLW +
Sbjct: 583  MVAGRFINCYATNDWTLAIAFRASLLSQGLAGIQPINGLGIENIDVTHLIEGHSSYLWAS 642

Query: 722  RLILELLELDTYYPVFRSFP 781
            +LILE LELDTYYPVFR  P
Sbjct: 643  QLILERLELDTYYPVFRRAP 662


>ref|XP_006425555.1| hypothetical protein CICLE_v10025097mg [Citrus clementina]
            gi|567865867|ref|XP_006425556.1| hypothetical protein
            CICLE_v10025097mg [Citrus clementina]
            gi|557527545|gb|ESR38795.1| hypothetical protein
            CICLE_v10025097mg [Citrus clementina]
            gi|557527546|gb|ESR38796.1| hypothetical protein
            CICLE_v10025097mg [Citrus clementina]
          Length = 662

 Score =  391 bits (1004), Expect = e-106
 Identities = 195/260 (75%), Positives = 218/260 (83%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GEN NQGRLAV IL+SGVVF+ EDFV+PWEGQ DN+ERY LQWESKNLIAVSTAIQDWLT
Sbjct: 403  GENQNQGRLAVEILISGVVFDQEDFVRPWEGQNDNMERYVLQWESKNLIAVSTAIQDWLT 462

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SR+++ELMK+GAMMTVL                 D IDSKWTIAVDRSDKAGKLLAEVL 
Sbjct: 463  SRIAMELMKQGAMMTVLKTLLAALAWPATLVFAADLIDSKWTIAVDRSDKAGKLLAEVLM 522

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            +GLQG RPVTLIG+SLGAR IFKCL+ LAE E NAG+VERVVLLGAP+SIK +NWE  RK
Sbjct: 523  QGLQGYRPVTLIGYSLGARVIFKCLENLAENECNAGIVERVVLLGAPISIKDQNWEAVRK 582

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRF+N Y+T+DWTL IAFRASLLS+GLAGI PI+  GIEN+DVT L+EGHSSYLW +
Sbjct: 583  MVAGRFINCYATNDWTLAIAFRASLLSQGLAGIQPINGLGIENIDVTHLIEGHSSYLWAS 642

Query: 722  RLILELLELDTYYPVFRSFP 781
            +LILE LELDTYYPVFR  P
Sbjct: 643  QLILERLELDTYYPVFRRAP 662


>ref|NP_001078500.2| uncharacterized protein [Arabidopsis thaliana]
            gi|332661235|gb|AEE86635.1| uncharacterized protein
            AT4G36210 [Arabidopsis thaliana]
          Length = 672

 Score =  390 bits (1003), Expect = e-106
 Identities = 193/257 (75%), Positives = 215/257 (83%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV +LV+GVVFE EDFVKPWEG   NLERY LQWESKNLI VSTAIQDWLT
Sbjct: 413  GENHNQGRLAVEVLVAGVVFEEEDFVKPWEGLTSNLERYTLQWESKNLILVSTAIQDWLT 472

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SRL++ELMK+GAM TVL+                DFIDSKW+IA+DRSDKAG+LLAEVL 
Sbjct: 473  SRLAMELMKQGAMHTVLASLLMALAWPATILVAADFIDSKWSIAIDRSDKAGRLLAEVLQ 532

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRP+TL+GFSLGAR IFKCLQ LAE E NA LVERVVLLGAP+SI  ENW   RK
Sbjct: 533  KGLQGNRPITLVGFSLGARVIFKCLQALAETEQNAELVERVVLLGAPISINSENWRDVRK 592

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRF+N Y+T+DWTLGIAFRASL+S+GLAGI PI + GIENVDVT++VEGHSSYLW T
Sbjct: 593  MVAGRFINVYATNDWTLGIAFRASLISQGLAGIQPICIPGIENVDVTDMVEGHSSYLWKT 652

Query: 722  RLILELLELDTYYPVFR 772
            + ILE LE+DTYYPVFR
Sbjct: 653  QQILERLEIDTYYPVFR 669


>ref|NP_849505.2| uncharacterized protein [Arabidopsis thaliana]
            gi|332661233|gb|AEE86633.1| uncharacterized protein
            AT4G36210 [Arabidopsis thaliana]
          Length = 550

 Score =  390 bits (1003), Expect = e-106
 Identities = 193/257 (75%), Positives = 215/257 (83%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV +LV+GVVFE EDFVKPWEG   NLERY LQWESKNLI VSTAIQDWLT
Sbjct: 291  GENHNQGRLAVEVLVAGVVFEEEDFVKPWEGLTSNLERYTLQWESKNLILVSTAIQDWLT 350

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SRL++ELMK+GAM TVL+                DFIDSKW+IA+DRSDKAG+LLAEVL 
Sbjct: 351  SRLAMELMKQGAMHTVLASLLMALAWPATILVAADFIDSKWSIAIDRSDKAGRLLAEVLQ 410

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRP+TL+GFSLGAR IFKCLQ LAE E NA LVERVVLLGAP+SI  ENW   RK
Sbjct: 411  KGLQGNRPITLVGFSLGARVIFKCLQALAETEQNAELVERVVLLGAPISINSENWRDVRK 470

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRF+N Y+T+DWTLGIAFRASL+S+GLAGI PI + GIENVDVT++VEGHSSYLW T
Sbjct: 471  MVAGRFINVYATNDWTLGIAFRASLISQGLAGIQPICIPGIENVDVTDMVEGHSSYLWKT 530

Query: 722  RLILELLELDTYYPVFR 772
            + ILE LE+DTYYPVFR
Sbjct: 531  QQILERLEIDTYYPVFR 547


>ref|XP_006343380.1| PREDICTED: transmembrane and coiled-coil domain-containing protein
            4-like isoform X3 [Solanum tuberosum]
          Length = 561

 Score =  390 bits (1002), Expect = e-106
 Identities = 190/256 (74%), Positives = 219/256 (85%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV IL+SG++FE EDF++PWEGQ DN ERY LQWESKNLIAVSTAIQDWLT
Sbjct: 299  GENHNQGRLAVQILISGLIFEEEDFIRPWEGQHDNSERYILQWESKNLIAVSTAIQDWLT 358

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SR+++ELMK+GAMMTVL                 DFIDS W+IAVDRSDKAG LLAEVL 
Sbjct: 359  SRIAMELMKQGAMMTVLHTLLIALAWPAALLTITDFIDSTWSIAVDRSDKAGVLLAEVLK 418

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQG+RPVTL+GFSLGAR IFKCLQ LAE E+N+GLVERVVLLGAP++IK  +WE ARK
Sbjct: 419  KGLQGHRPVTLVGFSLGARVIFKCLQVLAESENNSGLVERVVLLGAPIAIKDMDWEAARK 478

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            +VAGRFVNAYST+DW LG+AFRASLL++GLAGI P+++ G+ENVDVTE ++GHSSYLW T
Sbjct: 479  VVAGRFVNAYSTNDWMLGVAFRASLLTQGLAGIQPVEIPGVENVDVTEFIKGHSSYLWAT 538

Query: 722  RLILELLELDTYYPVF 769
            + ILELLELDTYYPVF
Sbjct: 539  QEILELLELDTYYPVF 554


>ref|XP_006343379.1| PREDICTED: transmembrane and coiled-coil domain-containing protein
            4-like isoform X2 [Solanum tuberosum]
          Length = 565

 Score =  390 bits (1002), Expect = e-106
 Identities = 190/256 (74%), Positives = 219/256 (85%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV IL+SG++FE EDF++PWEGQ DN ERY LQWESKNLIAVSTAIQDWLT
Sbjct: 303  GENHNQGRLAVQILISGLIFEEEDFIRPWEGQHDNSERYILQWESKNLIAVSTAIQDWLT 362

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SR+++ELMK+GAMMTVL                 DFIDS W+IAVDRSDKAG LLAEVL 
Sbjct: 363  SRIAMELMKQGAMMTVLHTLLIALAWPAALLTITDFIDSTWSIAVDRSDKAGVLLAEVLK 422

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQG+RPVTL+GFSLGAR IFKCLQ LAE E+N+GLVERVVLLGAP++IK  +WE ARK
Sbjct: 423  KGLQGHRPVTLVGFSLGARVIFKCLQVLAESENNSGLVERVVLLGAPIAIKDMDWEAARK 482

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            +VAGRFVNAYST+DW LG+AFRASLL++GLAGI P+++ G+ENVDVTE ++GHSSYLW T
Sbjct: 483  VVAGRFVNAYSTNDWMLGVAFRASLLTQGLAGIQPVEIPGVENVDVTEFIKGHSSYLWAT 542

Query: 722  RLILELLELDTYYPVF 769
            + ILELLELDTYYPVF
Sbjct: 543  QEILELLELDTYYPVF 558


>ref|XP_006343378.1| PREDICTED: transmembrane and coiled-coil domain-containing protein
            4-like isoform X1 [Solanum tuberosum]
          Length = 661

 Score =  390 bits (1002), Expect = e-106
 Identities = 190/256 (74%), Positives = 219/256 (85%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV IL+SG++FE EDF++PWEGQ DN ERY LQWESKNLIAVSTAIQDWLT
Sbjct: 399  GENHNQGRLAVQILISGLIFEEEDFIRPWEGQHDNSERYILQWESKNLIAVSTAIQDWLT 458

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SR+++ELMK+GAMMTVL                 DFIDS W+IAVDRSDKAG LLAEVL 
Sbjct: 459  SRIAMELMKQGAMMTVLHTLLIALAWPAALLTITDFIDSTWSIAVDRSDKAGVLLAEVLK 518

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQG+RPVTL+GFSLGAR IFKCLQ LAE E+N+GLVERVVLLGAP++IK  +WE ARK
Sbjct: 519  KGLQGHRPVTLVGFSLGARVIFKCLQVLAESENNSGLVERVVLLGAPIAIKDMDWEAARK 578

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            +VAGRFVNAYST+DW LG+AFRASLL++GLAGI P+++ G+ENVDVTE ++GHSSYLW T
Sbjct: 579  VVAGRFVNAYSTNDWMLGVAFRASLLTQGLAGIQPVEIPGVENVDVTEFIKGHSSYLWAT 638

Query: 722  RLILELLELDTYYPVF 769
            + ILELLELDTYYPVF
Sbjct: 639  QEILELLELDTYYPVF 654


>ref|XP_002869040.1| hypothetical protein ARALYDRAFT_912740 [Arabidopsis lyrata subsp.
            lyrata] gi|297314876|gb|EFH45299.1| hypothetical protein
            ARALYDRAFT_912740 [Arabidopsis lyrata subsp. lyrata]
          Length = 550

 Score =  390 bits (1001), Expect = e-106
 Identities = 193/257 (75%), Positives = 214/257 (83%)
 Frame = +2

Query: 2    GENHNQGRLAVGILVSGVVFEVEDFVKPWEGQEDNLERYALQWESKNLIAVSTAIQDWLT 181
            GENHNQGRLAV +LV+GVVFE EDFVKPWEG   NLERY LQWESKNLI VSTAIQDWLT
Sbjct: 291  GENHNQGRLAVEVLVAGVVFEEEDFVKPWEGLTSNLERYTLQWESKNLILVSTAIQDWLT 350

Query: 182  SRLSLELMKRGAMMTVLSXXXXXXXXXXXXXXXXDFIDSKWTIAVDRSDKAGKLLAEVLS 361
            SRL++ELMK+GAM TVL+                DFIDSKW+IA+DRSDKAG+LLAEVL 
Sbjct: 351  SRLAMELMKQGAMHTVLASLLMALAWPATILVAADFIDSKWSIAIDRSDKAGRLLAEVLQ 410

Query: 362  KGLQGNRPVTLIGFSLGARAIFKCLQCLAEMEDNAGLVERVVLLGAPVSIKGENWETARK 541
            KGLQGNRP+TL+GFSLGAR IFKCLQ LAE E NA LVERVVLLGAP+SI  ENW   RK
Sbjct: 411  KGLQGNRPITLVGFSLGARVIFKCLQALAETEQNAELVERVVLLGAPISINNENWRDVRK 470

Query: 542  MVAGRFVNAYSTSDWTLGIAFRASLLSKGLAGIGPIDVSGIENVDVTELVEGHSSYLWTT 721
            MVAGRF+N Y+T+DWTLGIAFRASL S+GLAGI PI + GIENVDVT++VEGHSSYLW T
Sbjct: 471  MVAGRFINVYATNDWTLGIAFRASLFSQGLAGIQPICIPGIENVDVTDMVEGHSSYLWKT 530

Query: 722  RLILELLELDTYYPVFR 772
            + ILE LE+DTYYPVFR
Sbjct: 531  QQILERLEIDTYYPVFR 547