BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia25_contig00003936
         (1134 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI40669.3| unnamed protein product [Vitis vinifera]              396   e-108
emb|CAN77424.1| hypothetical protein VITISV_016423 [Vitis vinifera]   396   e-107
ref|XP_002265964.2| PREDICTED: isoamylase 1, chloroplastic-like ...   387   e-105
ref|XP_007014466.1| Isoamylase 1 isoform 2 [Theobroma cacao] gi|...   372   e-100
ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum] gi|...   367   7e-99
ref|XP_007225082.1| hypothetical protein PRUPE_ppa025097mg, part...   361   4e-97
ref|XP_007160229.1| hypothetical protein PHAVU_002G303600g [Phas...   360   5e-97
ref|XP_003532455.2| PREDICTED: isoamylase 1, chloroplastic-like ...   358   2e-96
ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic-like ...   358   2e-96
ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like ...   354   3e-95
ref|XP_006453203.1| hypothetical protein CICLE_v100074892mg, par...   354   3e-95
ref|XP_004163012.1| PREDICTED: isoamylase 1, chloroplastic-like ...   352   2e-94
ref|XP_004149676.1| PREDICTED: isoamylase 1, chloroplastic-like ...   352   2e-94
gb|AAY84833.1| isoamylase [Ipomoea batatas]                           350   5e-94
ref|XP_002324659.2| isoamylase family protein [Populus trichocar...   350   8e-94
ref|XP_004301409.1| PREDICTED: isoamylase 1, chloroplastic-like ...   350   8e-94
gb|AAZ81835.1| isoamylase isoform 1 [Pisum sativum]                   347   4e-93
gb|EXC12839.1| Isoamylase 1 [Morus notabilis]                         347   7e-93
ref|XP_002308090.2| isoamylase family protein [Populus trichocar...   347   7e-93
ref|XP_006381137.1| hypothetical protein POPTR_0006s07010g [Popu...   347   7e-93

>emb|CBI40669.3| unnamed protein product [Vitis vinifera]
          Length = 809

 Score =  396 bits (1018), Expect = e-108
 Identities = 202/297 (68%), Positives = 224/297 (75%), Gaps = 8/297 (2%)
 Frame = -1

Query: 867 MELVQNASCLVYSLNYNPWKVKWRNLLFSHKFQQ--------KPSRNHKIWSKNSMNIRA 712
           MEL   ASC   S +Y P       LL  HK  Q        K S N   WS    N+R 
Sbjct: 11  MELTHYASC---SSHYLP------KLLSPHKPPQWWNPTPRGKSSNN---WSAKPSNMRF 58

Query: 711 MKSFVVSASDGGRAEVAETAVIEKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVS 532
            K  V +   G  AE   T V+EKP+L+P  VF G P P GATARD GVNFA+YS  AVS
Sbjct: 59  SKWVVAAVGSGAEAE---TVVVEKPKLQPFLVFEGCPAPLGATARDGGVNFAVYSGNAVS 115

Query: 531 ATLCLISLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGH 352
           ATLCLIS SDL+E+RVTE+I+LD L NKTGDVWH FLKG+FEN++YGY+FDGK S EEGH
Sbjct: 116 ATLCLISASDLEEDRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGH 175

Query: 351 YYDSSRILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKD 172
           YYDSSR+L+DPYAKAVISRGE+G LGPEGNCWP MAGM+P+SD  FDWEGDLPLKYPQKD
Sbjct: 176 YYDSSRLLLDPYAKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKD 235

Query: 171 LIIYEMHVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           LIIYEMHVRGFTRH+SSRT FPGTY GVVEKLDH KELGVNCIELMPC E+NELEYF
Sbjct: 236 LIIYEMHVRGFTRHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYF 292


>emb|CAN77424.1| hypothetical protein VITISV_016423 [Vitis vinifera]
          Length = 440

 Score =  396 bits (1017), Expect = e-107
 Identities = 202/297 (68%), Positives = 224/297 (75%), Gaps = 8/297 (2%)
 Frame = -1

Query: 867 MELVQNASCLVYSLNYNPWKVKWRNLLFSHKFQQ--------KPSRNHKIWSKNSMNIRA 712
           MEL   ASC   S +Y P       LL  HK  Q        K S N   WS    N+R 
Sbjct: 1   MELTHYASC---SSHYLP------KLLSPHKPXQWWNPTPRGKSSNN---WSAKPSNMRF 48

Query: 711 MKSFVVSASDGGRAEVAETAVIEKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVS 532
            K  V +   G  AE   T V+EKP+L+P  VF G P P GATARD GVNFA+YS  AVS
Sbjct: 49  SKWVVAAVGSGAEAE---TVVVEKPKLQPFLVFEGCPAPLGATARDGGVNFAVYSGNAVS 105

Query: 531 ATLCLISLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGH 352
           ATLCLIS SDL+E+RVTE+I+LD L NKTGDVWH FLKG+FEN++YGY+FDGK S EEGH
Sbjct: 106 ATLCLISASDLEEDRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGH 165

Query: 351 YYDSSRILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKD 172
           YYDSSR+L+DPYAKAVISRGE+G LGPEGNCWP MAGM+P+SD  FDWEGDLPLKYPQKD
Sbjct: 166 YYDSSRMLLDPYAKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKD 225

Query: 171 LIIYEMHVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           LIIYEMHVRGFTRH+SSRT FPGTY GVVEKLDH KELGVNCIELMPC E+NELEYF
Sbjct: 226 LIIYEMHVRGFTRHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYF 282


>ref|XP_002265964.2| PREDICTED: isoamylase 1, chloroplastic-like [Vitis vinifera]
          Length = 748

 Score =  387 bits (993), Expect = e-105
 Identities = 183/232 (78%), Positives = 203/232 (87%)
 Frame = -1

Query: 696 VSASDGGRAEVAETAVIEKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCL 517
           V A+ G  AE AET V+EKP+L+P  VF G P P GATARD GVNFA+YS  AVSATLCL
Sbjct: 7   VVAAVGSGAE-AETVVVEKPKLQPFLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCL 65

Query: 516 ISLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSS 337
           IS SDL+E+RVTE+I+LD L NKTGDVWH FLKG+FEN++YGY+FDGK S EEGHYYDSS
Sbjct: 66  ISASDLEEDRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGHYYDSS 125

Query: 336 RILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIYE 157
           R+L+DPYAKAVISRGE+G LGPEGNCWP MAGM+P+SD  FDWEGDLPLKYPQKDLIIYE
Sbjct: 126 RLLLDPYAKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKDLIIYE 185

Query: 156 MHVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           MHVRGFTRH+SSRT FPGTY GVVEKLDH KELGVNCIELMPC E+NELEYF
Sbjct: 186 MHVRGFTRHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYF 237


>ref|XP_007014466.1| Isoamylase 1 isoform 2 [Theobroma cacao]
           gi|508784829|gb|EOY32085.1| Isoamylase 1 isoform 2
           [Theobroma cacao]
          Length = 795

 Score =  372 bits (954), Expect = e-100
 Identities = 182/287 (63%), Positives = 217/287 (75%), Gaps = 5/287 (1%)
 Frame = -1

Query: 846 SCLVYSLNYNPWKVKWRNLLFSHKFQQKPSRNHKIWSKNSMNIRAMKSFVVSASDGGR-- 673
           +C  + L Y P     + +  + + Q  P  N +  S N       +  V +   GG   
Sbjct: 4   TCCTFCLQYAP-----KLIPATLRRQTPPKPNLRTVSNNHATTSTTRMVVKARGGGGGGA 58

Query: 672 ---AEVAETAVIEKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCLISLSD 502
              A  A+TAV+EKP+++  ++  G P PFGAT  D GVNFAIYS+ AVSATLCLI+ SD
Sbjct: 59  RRGAAEADTAVVEKPKIKRFQLSEGHPGPFGATLLDGGVNFAIYSANAVSATLCLITFSD 118

Query: 501 LQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSSRILVD 322
           LQ+NRVTE+I+LD L NKTGDVWH FL+GDF++MLYGY FDGK S E+GHYYDSS+IL+D
Sbjct: 119 LQQNRVTEQISLDPLTNKTGDVWHVFLRGDFKDMLYGYIFDGKFSPEKGHYYDSSKILLD 178

Query: 321 PYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIYEMHVRG 142
           PYAKAVISRGE+GALGPE NCWP MA MVPTS++ FDWEGDLPL+Y Q+DLIIYEMHVRG
Sbjct: 179 PYAKAVISRGEFGALGPEDNCWPQMACMVPTSEDEFDWEGDLPLRYSQRDLIIYEMHVRG 238

Query: 141 FTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           FTRH+SSRT FPGTY GVVEKLDH KELGVNCIELMPC E+NELEY+
Sbjct: 239 FTRHESSRTEFPGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEYY 285


>ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum] gi|27728145|gb|AAN15317.1|
           isoamylase isoform 1 [Solanum tuberosum]
          Length = 793

 Score =  367 bits (941), Expect = 7e-99
 Identities = 179/280 (63%), Positives = 213/280 (76%), Gaps = 6/280 (2%)
 Frame = -1

Query: 822 YNPWKVKWRNLLFSHKFQQKPSRNHKIWSKNSMNIRAMKSFVVSASDGGRAEVAETA--- 652
           Y P K+ + N LFS +          IW K+  ++      V +A D GR  V +TA   
Sbjct: 12  YKP-KLSFHNHLFSRRSSNGVDFE-SIWRKSRSSV------VNAAVDSGRGGVVKTAATA 63

Query: 651 -VIEKPRLRP--LEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCLISLSDLQENRVT 481
            V+EKP       EV  G P+PFGATA D GVNFA++S  A +ATLCLI+LSDL E RVT
Sbjct: 64  VVVEKPTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSDLPEKRVT 123

Query: 480 ERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSSRILVDPYAKAVI 301
           E+I LD L NKTGDVWH FLKGDFENMLYGY+FDGK   EEGHY+DSS+I++DPYAKA++
Sbjct: 124 EQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLDPYAKAIV 183

Query: 300 SRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIYEMHVRGFTRHDSS 121
           SRGEYG LGPE +CWPPMAGMVP++ ++FDWEGDLPLK+PQ+DL+IYEMHVRGFT H+SS
Sbjct: 184 SRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFPQRDLVIYEMHVRGFTNHESS 243

Query: 120 RTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
            T +PGTYLGVVEKLDH KELGVNCIELMPC E+NELEY+
Sbjct: 244 ETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYY 283


>ref|XP_007225082.1| hypothetical protein PRUPE_ppa025097mg, partial [Prunus persica]
           gi|462422018|gb|EMJ26281.1| hypothetical protein
           PRUPE_ppa025097mg, partial [Prunus persica]
          Length = 777

 Score =  361 bits (926), Expect = 4e-97
 Identities = 166/234 (70%), Positives = 198/234 (84%), Gaps = 1/234 (0%)
 Frame = -1

Query: 699 VVSASDGGRA-EVAETAVIEKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATL 523
           VV AS+GG   +V ETAV+++P+LR  +V  G P PFGAT RD GVNFA+YS+ AVS TL
Sbjct: 9   VVKASNGGAGTDVVETAVVDRPKLRRFQVSQGHPSPFGATTRDGGVNFAVYSANAVSVTL 68

Query: 522 CLISLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYD 343
           CLI+LSDL+E++VTE+++LD L NKTG+VWH FLKGDF++ LYGY+F+GK S EEG YYD
Sbjct: 69  CLITLSDLEEDKVTEQLSLDPLTNKTGNVWHVFLKGDFKDTLYGYKFEGKFSPEEGLYYD 128

Query: 342 SSRILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLII 163
           SS+I++DPYAKAVI RGEYG L P+GNCWP MAG VP+ D+ FDWEGDLPL YPQKDLII
Sbjct: 129 SSKIVLDPYAKAVIRRGEYGKLSPDGNCWPQMAGTVPSFDDEFDWEGDLPLNYPQKDLII 188

Query: 162 YEMHVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           YE+HVRGFTRH+SS    PGTYLG+VEKLDH KELG+NCIELMPC E+NELEYF
Sbjct: 189 YEIHVRGFTRHESSMAELPGTYLGLVEKLDHLKELGINCIELMPCHEFNELEYF 242


>ref|XP_007160229.1| hypothetical protein PHAVU_002G303600g [Phaseolus vulgaris]
           gi|139867053|dbj|BAF52941.1| isoamylase-type
           starch-debranching enzyme 1 [Phaseolus vulgaris]
           gi|561033644|gb|ESW32223.1| hypothetical protein
           PHAVU_002G303600g [Phaseolus vulgaris]
          Length = 791

 Score =  360 bits (925), Expect = 5e-97
 Identities = 170/230 (73%), Positives = 190/230 (82%), Gaps = 1/230 (0%)
 Frame = -1

Query: 690 ASDGGRAEVAETAVIEKPRLRPL-EVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCLI 514
           A+  G A   ETAV+EKP+L  L +V  G   PFGAT RD GVNFAIYS  A SATLCL 
Sbjct: 51  AARNGGAVDTETAVVEKPQLESLFQVSRGYASPFGATVRDGGVNFAIYSLNAFSATLCLF 110

Query: 513 SLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSSR 334
           +LSD Q NRVTE + LD LINKTG +WH FLKGDF +MLYGY+FDGK S  EGHYYDSSR
Sbjct: 111 TLSDFQNNRVTESVPLDPLINKTGGIWHVFLKGDFSDMLYGYKFDGKFSPLEGHYYDSSR 170

Query: 333 ILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIYEM 154
           I++DPYAKAVISRGE+GALGP GNCWP MAGMVP+  + FDWEGDLPLKYPQKDL++YEM
Sbjct: 171 IVLDPYAKAVISRGEFGALGPNGNCWPQMAGMVPSDHDEFDWEGDLPLKYPQKDLVVYEM 230

Query: 153 HVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEY 4
           HVRGFT+H+SS T FPGTYLGVVEKLDH KELGVNC+ELMPC E+NELEY
Sbjct: 231 HVRGFTKHESSNTKFPGTYLGVVEKLDHLKELGVNCLELMPCHEFNELEY 280


>ref|XP_003532455.2| PREDICTED: isoamylase 1, chloroplastic-like [Glycine max]
          Length = 798

 Score =  358 bits (920), Expect = 2e-96
 Identities = 169/232 (72%), Positives = 194/232 (83%), Gaps = 1/232 (0%)
 Frame = -1

Query: 693 SASDGGRAEVAETAVIEKPRLRPL-EVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCL 517
           +A++GG  E  ETAV+EKP+L+ L +   G P PFGAT RD GVNFAI S  A+SATLC 
Sbjct: 58  AAANGGGFET-ETAVVEKPQLKTLFQASRGYPSPFGATVRDGGVNFAISSLNALSATLCF 116

Query: 516 ISLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSS 337
            +LSD Q NRVTE + LD LIN+TG VWH FLKGDF +MLYGY+FDGK S  EGHY+DSS
Sbjct: 117 FTLSDFQNNRVTEYVPLDPLINRTGGVWHVFLKGDFRDMLYGYKFDGKFSPLEGHYFDSS 176

Query: 336 RILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIYE 157
            IL+DPYAKAVISRGE+GALGP+GNCWP MAG VP+ D+ FDWEGDLPLKYPQKDL+IYE
Sbjct: 177 HILLDPYAKAVISRGEFGALGPDGNCWPQMAGTVPSEDDEFDWEGDLPLKYPQKDLVIYE 236

Query: 156 MHVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           MHVRGFT+H+SS T FPGTYLGVVEKLDH KELGVNC+ELMPC E+NELEY+
Sbjct: 237 MHVRGFTKHESSNTKFPGTYLGVVEKLDHLKELGVNCLELMPCHEFNELEYY 288


>ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic-like [Solanum lycopersicum]
          Length = 787

 Score =  358 bits (919), Expect = 2e-96
 Identities = 176/289 (60%), Positives = 215/289 (74%)
 Frame = -1

Query: 867 MELVQNASCLVYSLNYNPWKVKWRNLLFSHKFQQKPSRNHKIWSKNSMNIRAMKSFVVSA 688
           MEL+ + S   Y       K+ ++N LFS +   KP     I  K+ +N    +   ++A
Sbjct: 1   MELLHSPSISTYKP-----KLNYQNHLFSRR-NSKPVDFETIRRKSVVNAAVHR---LAA 51

Query: 687 SDGGRAEVAETAVIEKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCLISL 508
                A V E +  E+ R    EV  G P+PFGATA D GVNFA++S  A +ATLCLI+L
Sbjct: 52  KTAASAVVVEKSTTERCRF---EVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITL 108

Query: 507 SDLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSSRIL 328
           SDL E RVTE+I LD L NKTGDVWH FLKGDFENMLYGY+FDGK   EEGHY+DSS+I+
Sbjct: 109 SDLPEKRVTEQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIV 168

Query: 327 VDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIYEMHV 148
           +DPYAKA++SRGEYG LGPE +CWPPMAGMVP++ ++FDWEGDLPLK+ Q++L+IYEMHV
Sbjct: 169 LDPYAKAIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFSQRNLVIYEMHV 228

Query: 147 RGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           RGFT H+SS T +PGTYLGVVEKLDH KELGVNCIELMPC E+NELEY+
Sbjct: 229 RGFTNHESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYY 277


>ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like [Citrus sinensis]
          Length = 805

 Score =  354 bits (909), Expect = 3e-95
 Identities = 168/255 (65%), Positives = 205/255 (80%), Gaps = 4/255 (1%)
 Frame = -1

Query: 753 NHKIWSKNSMNIRAM----KSFVVSASDGGRAEVAETAVIEKPRLRPLEVFGGSPMPFGA 586
           NH   + +  N +A     K+ V+ AS   ++   ETAVIEKP  +  +V  G P PFGA
Sbjct: 44  NHPNKTSHFANSKAFENVTKNLVIRAS---KSAELETAVIEKPPSQRFQVSKGYPTPFGA 100

Query: 585 TARDSGVNFAIYSSGAVSATLCLISLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFE 406
           T RD GVNF+I+SS AVSA+LCLI+LSDLQEN+VTE I LDS  NKTGDVWH FLKGDF+
Sbjct: 101 TLRDGGVNFSIFSSNAVSASLCLITLSDLQENKVTEEIALDSFANKTGDVWHVFLKGDFK 160

Query: 405 NMLYGYEFDGKISSEEGHYYDSSRILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTS 226
           +MLYGY+FDGK S +EGHY+D ++I++DPYAKAVISR ++G LGP+ NCWP MA +VPT 
Sbjct: 161 DMLYGYKFDGKFSPQEGHYFDPTKIVLDPYAKAVISRAQFGVLGPDENCWPQMACLVPTP 220

Query: 225 DNRFDWEGDLPLKYPQKDLIIYEMHVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNC 46
           ++ FDWEGDLPLKYPQ+DLIIYE+HVRGFTRH+SS+T  PGTYLGVVEKLDH K+LG+NC
Sbjct: 221 EDEFDWEGDLPLKYPQRDLIIYEVHVRGFTRHESSKTEHPGTYLGVVEKLDHLKDLGINC 280

Query: 45  IELMPCQEYNELEYF 1
           +ELMPC E+NELEYF
Sbjct: 281 LELMPCHEFNELEYF 295


>ref|XP_006453203.1| hypothetical protein CICLE_v100074892mg, partial [Citrus
           clementina] gi|557556429|gb|ESR66443.1| hypothetical
           protein CICLE_v100074892mg, partial [Citrus clementina]
          Length = 624

 Score =  354 bits (909), Expect = 3e-95
 Identities = 168/255 (65%), Positives = 205/255 (80%), Gaps = 4/255 (1%)
 Frame = -1

Query: 753 NHKIWSKNSMNIRAM----KSFVVSASDGGRAEVAETAVIEKPRLRPLEVFGGSPMPFGA 586
           NH   + +  N +A     K+ V+ AS   ++   ETAVIEKP  +  +V  G P PFGA
Sbjct: 44  NHPNKTSHFANSKAFENVTKNLVIRAS---KSAELETAVIEKPPSQRFQVSKGYPTPFGA 100

Query: 585 TARDSGVNFAIYSSGAVSATLCLISLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFE 406
           T RD GVNF+I+SS AVSA+LCLI+LSDLQEN+VTE I LDS  NKTGDVWH FLKGDF+
Sbjct: 101 TLRDGGVNFSIFSSNAVSASLCLITLSDLQENKVTEEIALDSFANKTGDVWHVFLKGDFK 160

Query: 405 NMLYGYEFDGKISSEEGHYYDSSRILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTS 226
           +MLYGY+FDGK S +EGHY+D ++I++DPYAKAVISR ++G LGP+ NCWP MA +VPT 
Sbjct: 161 DMLYGYKFDGKFSPQEGHYFDPTKIVLDPYAKAVISRAQFGVLGPDENCWPQMACLVPTP 220

Query: 225 DNRFDWEGDLPLKYPQKDLIIYEMHVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNC 46
           ++ FDWEGDLPLKYPQ+DLIIYE+HVRGFTRH+SS+T  PGTYLGVVEKLDH K+LG+NC
Sbjct: 221 EDEFDWEGDLPLKYPQRDLIIYEVHVRGFTRHESSKTEHPGTYLGVVEKLDHLKDLGINC 280

Query: 45  IELMPCQEYNELEYF 1
           +ELMPC E+NELEYF
Sbjct: 281 LELMPCHEFNELEYF 295


>ref|XP_004163012.1| PREDICTED: isoamylase 1, chloroplastic-like [Cucumis sativus]
          Length = 810

 Score =  352 bits (903), Expect = 2e-94
 Identities = 166/228 (72%), Positives = 190/228 (83%), Gaps = 3/228 (1%)
 Frame = -1

Query: 675 RAEVAETAVIEKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCLISLSDLQ 496
           R  VA    +E   L   EVF G+P PFGATAR+ G+NFAIYS+ AVSATLCLI LSDLQ
Sbjct: 70  RTSVAVAEEVEVSNLNRFEVFDGTPTPFGATAREDGINFAIYSANAVSATLCLIHLSDLQ 129

Query: 495 ENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSSRILVDPY 316
           ENRVT+ I LD L NKTGDVWH FLKGDF  MLYGY+FDGK S +EG Y+DSSRIL+DPY
Sbjct: 130 ENRVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDSSRILLDPY 189

Query: 315 AKAVISRGEYGALGPEGNCWPPMAGMVPTSDNR---FDWEGDLPLKYPQKDLIIYEMHVR 145
           AKAVISRGE+GALGP  NCWP MAGMVPT++ +   FDWEGDLP+++ QKDLIIYEMHVR
Sbjct: 190 AKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLPVQHAQKDLIIYEMHVR 249

Query: 144 GFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           GFTRH+SS++ FPGTYLG+VEKLD+ KELG+NCIELMPC E+NELEYF
Sbjct: 250 GFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNELEYF 297


>ref|XP_004149676.1| PREDICTED: isoamylase 1, chloroplastic-like [Cucumis sativus]
          Length = 810

 Score =  352 bits (903), Expect = 2e-94
 Identities = 166/228 (72%), Positives = 190/228 (83%), Gaps = 3/228 (1%)
 Frame = -1

Query: 675 RAEVAETAVIEKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCLISLSDLQ 496
           R  VA    +E   L   EVF G+P PFGATAR+ G+NFAIYS+ AVSATLCLI LSDLQ
Sbjct: 70  RTSVAVAEEVEVSNLNRFEVFDGTPTPFGATAREDGINFAIYSANAVSATLCLIHLSDLQ 129

Query: 495 ENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSSRILVDPY 316
           ENRVT+ I LD L NKTGDVWH FLKGDF  MLYGY+FDGK S +EG Y+DSSRIL+DPY
Sbjct: 130 ENRVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDSSRILLDPY 189

Query: 315 AKAVISRGEYGALGPEGNCWPPMAGMVPTSDNR---FDWEGDLPLKYPQKDLIIYEMHVR 145
           AKAVISRGE+GALGP  NCWP MAGMVPT++ +   FDWEGDLP+++ QKDLIIYEMHVR
Sbjct: 190 AKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLPVQHAQKDLIIYEMHVR 249

Query: 144 GFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           GFTRH+SS++ FPGTYLG+VEKLD+ KELG+NCIELMPC E+NELEYF
Sbjct: 250 GFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNELEYF 297


>gb|AAY84833.1| isoamylase [Ipomoea batatas]
          Length = 785

 Score =  350 bits (899), Expect = 5e-94
 Identities = 177/293 (60%), Positives = 210/293 (71%), Gaps = 4/293 (1%)
 Frame = -1

Query: 867 MELVQNASCLVYSLNYNPWKVKWRNLLFSHKFQQKPSRNHKIWSKNSMNIRAMKSFVVSA 688
           MEL+ + S   YS   +      R    S  F       H  +++     R     V +A
Sbjct: 1   MELLHSPSISQYSPKLHSRSSNIRKTGLSFGFP------HNYFAEFEKKARPSALAVNAA 54

Query: 687 SDGGRAEVAETAVIEKPR----LRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLC 520
            DGG  E     V+EKP     LR  EV  G P PFGATARD G+NFA++SS A SA LC
Sbjct: 55  IDGG-GESDTAVVVEKPLPYGLLRRFEVLSGHPAPFGATARDGGINFAVFSSNATSAALC 113

Query: 519 LISLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDS 340
           LISL+DL E +VTE+I LD  INKTGDVWH FL+GDF+NMLYGY FDGK + EEGHY+DS
Sbjct: 114 LISLADLPEKKVTEQIPLDPSINKTGDVWHVFLQGDFDNMLYGYSFDGKFAPEEGHYFDS 173

Query: 339 SRILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIY 160
           +RIL+DPYAKA++SR E+GALGPE +CWPPMA M+PT+D +FDWEGDLPLK+PQ+DL+IY
Sbjct: 174 ARILLDPYAKAIVSRAEFGALGPEKDCWPPMACMLPTAD-KFDWEGDLPLKFPQRDLVIY 232

Query: 159 EMHVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           EMHVRGFT H+SS T FPGTY GVVEKLDH K LGVNCIELMPC E+NELEY+
Sbjct: 233 EMHVRGFTNHESSGTEFPGTYRGVVEKLDHLKVLGVNCIELMPCHEFNELEYY 285


>ref|XP_002324659.2| isoamylase family protein [Populus trichocarpa]
           gi|550318648|gb|EEF03224.2| isoamylase family protein
           [Populus trichocarpa]
          Length = 794

 Score =  350 bits (897), Expect = 8e-94
 Identities = 166/236 (70%), Positives = 194/236 (82%), Gaps = 3/236 (1%)
 Frame = -1

Query: 699 VVSASDGGRAEVAETAVI---EKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSA 529
           + +A DG      +TAV+   E P+LR  +VF G P PFGAT RD GVNFAI+S+ AVSA
Sbjct: 54  IKAARDG-----VDTAVVVEEEGPKLRKFQVFEGHPSPFGATVRDGGVNFAIFSANAVSA 108

Query: 528 TLCLISLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHY 349
           TLCLISLSDL ENRVTE+  LD L NKTGDVWH  LKGDF++MLYGY+FDG  S EEGHY
Sbjct: 109 TLCLISLSDLPENRVTEQFFLDPLTNKTGDVWHVHLKGDFKDMLYGYKFDGSFSPEEGHY 168

Query: 348 YDSSRILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDL 169
           YD S+I++DPYAKAVISRG++G LG + NCWP MA M+PT+D++FDWEGD  LKYPQ+DL
Sbjct: 169 YDPSQIVLDPYAKAVISRGDFGVLGSDDNCWPQMACMIPTADDKFDWEGDSLLKYPQRDL 228

Query: 168 IIYEMHVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           I+YEMHVRGFT+H+SSRT FPGTYLGVVEKL H KELGVNCIELMPC E+NELEY+
Sbjct: 229 ILYEMHVRGFTQHESSRTEFPGTYLGVVEKLGHLKELGVNCIELMPCHEFNELEYY 284


>ref|XP_004301409.1| PREDICTED: isoamylase 1, chloroplastic-like [Fragaria vesca subsp.
           vesca]
          Length = 794

 Score =  350 bits (897), Expect = 8e-94
 Identities = 172/273 (63%), Positives = 209/273 (76%), Gaps = 10/273 (3%)
 Frame = -1

Query: 789 LFSHKFQQKPSRNHKIWSKNSMNIRAMKSF-----VVSASDGG----RAEVAETAVIEK- 640
           L+S     + + N ++   N +N R M  F      V A+DGG     AE  ETAV+E+ 
Sbjct: 13  LYSPHHHNRFASNARLPGPNRLN-RPMLGFRRHAPPVKANDGGGAGTTAEAVETAVVERS 71

Query: 639 PRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCLISLSDLQENRVTERINLDS 460
           P+LR  +V  G P PFGAT  D GVNFA+Y+  AVSAT+CLI+ +DL++++VTE+I LD 
Sbjct: 72  PKLRRFQVSDGRPNPFGATPCDGGVNFAVYAGNAVSATVCLITPADLEQDKVTEQIPLDP 131

Query: 459 LINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSSRILVDPYAKAVISRGEYGA 280
           L NKTGDVWH FL GD +++LYGY+FDGK S EEGHYYD S+I++DPYAKAVI RGE+G 
Sbjct: 132 LNNKTGDVWHVFLTGDVKDILYGYKFDGKFSPEEGHYYDPSKIVMDPYAKAVIRRGEFGN 191

Query: 279 LGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIYEMHVRGFTRHDSSRTNFPGT 100
           LGP GNCWP MA MVP+ D++F+WEGDLPL YPQKDLIIYEMHVRGFTRH+SS T  PGT
Sbjct: 192 LGPNGNCWPQMACMVPSFDDKFNWEGDLPLNYPQKDLIIYEMHVRGFTRHESSMTELPGT 251

Query: 99  YLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           YLG+VEKLDH KELG+NCIELMPC E+NELEYF
Sbjct: 252 YLGLVEKLDHLKELGINCIELMPCHEFNELEYF 284


>gb|AAZ81835.1| isoamylase isoform 1 [Pisum sativum]
          Length = 791

 Score =  347 bits (891), Expect = 4e-93
 Identities = 173/269 (64%), Positives = 203/269 (75%), Gaps = 5/269 (1%)
 Frame = -1

Query: 792 LLFSHKFQQKPSRNHK-----IWSKNSMNIRAMKSFVVSASDGGRAEVAETAVIEKPRLR 628
           L+   K  +  + NH      I SK++ N    K+F +    G   E+A    I KP+  
Sbjct: 22  LINKTKLLELHNHNHSRISITISSKSNSN----KTFAIGNRVGVETEIA-VVEIHKPQF- 75

Query: 627 PLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCLISLSDLQENRVTERINLDSLINK 448
             EV  G P PFGATA++ GVNFAIYS  A SATLCL +LSD + N+VTE I LD L+NK
Sbjct: 76  --EVSRGFPSPFGATAQEDGVNFAIYSLNAHSATLCLFTLSDFKNNKVTEYIALDPLVNK 133

Query: 447 TGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSSRILVDPYAKAVISRGEYGALGPE 268
           TG VWH FLKGDF++MLYGY+FDGK S ++GHYYDSSR+L+DPYAKAVISRGEYGA+G +
Sbjct: 134 TGCVWHVFLKGDFKDMLYGYKFDGKFSPQQGHYYDSSRVLLDPYAKAVISRGEYGAVGLD 193

Query: 267 GNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIYEMHVRGFTRHDSSRTNFPGTYLGV 88
           GNCWP MAGMVP     FDWEGDLPLKYPQKDLIIYEMHVRGFT+H+SS+T FPGTYLGV
Sbjct: 194 GNCWPQMAGMVPFDPEEFDWEGDLPLKYPQKDLIIYEMHVRGFTKHESSKTEFPGTYLGV 253

Query: 87  VEKLDHFKELGVNCIELMPCQEYNELEYF 1
           VEKLDH  ELGVNCI+LMPC E+NELEY+
Sbjct: 254 VEKLDHLTELGVNCIQLMPCNEFNELEYY 282


>gb|EXC12839.1| Isoamylase 1 [Morus notabilis]
          Length = 805

 Score =  347 bits (889), Expect = 7e-93
 Identities = 163/234 (69%), Positives = 192/234 (82%), Gaps = 1/234 (0%)
 Frame = -1

Query: 699 VVSASDGGRAEVAETAVIEK-PRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATL 523
           +V A  GG +  AE AV++  P+L   +V  GSP+P GAT RD GVNFA+++  AV ATL
Sbjct: 50  IVKAGRGGGS--AEVAVVDGGPQLSRFQVSRGSPVPLGATLRDGGVNFAVFAGNAVKATL 107

Query: 522 CLISLSDLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYD 343
           CL+SLSDL++NRVTE I+LD +IN+TG VWH F+K DF++MLYGY+FDGK S EEGHYYD
Sbjct: 108 CLMSLSDLEQNRVTEEISLDPVINQTGSVWHVFVKVDFKDMLYGYKFDGKFSPEEGHYYD 167

Query: 342 SSRILVDPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLII 163
            SRI++DPY KAVI RGEYG LGP+GNCWP MA  VP  DN FDWEGDLPLKYPQ+DLI+
Sbjct: 168 PSRIVLDPYGKAVICRGEYGTLGPDGNCWPQMACAVPFFDNEFDWEGDLPLKYPQEDLIV 227

Query: 162 YEMHVRGFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           YEMHVRGFTRH+SS T FPGTYLGVVEKL H +ELGVNCIELMPC E+NELEY+
Sbjct: 228 YEMHVRGFTRHESSGTEFPGTYLGVVEKLHHLQELGVNCIELMPCHEFNELEYY 281


>ref|XP_002308090.2| isoamylase family protein [Populus trichocarpa]
           gi|550335674|gb|EEE91613.2| isoamylase family protein
           [Populus trichocarpa]
          Length = 876

 Score =  347 bits (889), Expect = 7e-93
 Identities = 163/228 (71%), Positives = 193/228 (84%), Gaps = 4/228 (1%)
 Frame = -1

Query: 672 AEVAETAVI----EKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCLISLS 505
           +E  +TAV+    E+P+L+ ++VF G P PFGAT RD GVNFAI+S+ AVSATLCLISLS
Sbjct: 108 SEGVDTAVVVVEEEEPKLKKIQVFEGHPAPFGATVRDGGVNFAIFSADAVSATLCLISLS 167

Query: 504 DLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSSRILV 325
           DL ENRVTE+I LD L NKTGDVWH  LKGDF++MLYGY+FDG  S E G YYD S+I++
Sbjct: 168 DLPENRVTEQIFLDPLTNKTGDVWHVLLKGDFKDMLYGYKFDGNFSPEVGLYYDPSKIVL 227

Query: 324 DPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIYEMHVR 145
           DPYAK+VISRGE+G LG + N WP MA M+PT++N+FDWEGD PLK+PQ+DLIIYEMHVR
Sbjct: 228 DPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKHPQRDLIIYEMHVR 287

Query: 144 GFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           GFT+H+SSRT FPGTYLGVVEKLDH KELGVNCIELMPC E+NELEY+
Sbjct: 288 GFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYY 335


>ref|XP_006381137.1| hypothetical protein POPTR_0006s07010g [Populus trichocarpa]
           gi|550335673|gb|ERP58934.1| hypothetical protein
           POPTR_0006s07010g [Populus trichocarpa]
          Length = 845

 Score =  347 bits (889), Expect = 7e-93
 Identities = 163/228 (71%), Positives = 193/228 (84%), Gaps = 4/228 (1%)
 Frame = -1

Query: 672 AEVAETAVI----EKPRLRPLEVFGGSPMPFGATARDSGVNFAIYSSGAVSATLCLISLS 505
           +E  +TAV+    E+P+L+ ++VF G P PFGAT RD GVNFAI+S+ AVSATLCLISLS
Sbjct: 108 SEGVDTAVVVVEEEEPKLKKIQVFEGHPAPFGATVRDGGVNFAIFSADAVSATLCLISLS 167

Query: 504 DLQENRVTERINLDSLINKTGDVWHAFLKGDFENMLYGYEFDGKISSEEGHYYDSSRILV 325
           DL ENRVTE+I LD L NKTGDVWH  LKGDF++MLYGY+FDG  S E G YYD S+I++
Sbjct: 168 DLPENRVTEQIFLDPLTNKTGDVWHVLLKGDFKDMLYGYKFDGNFSPEVGLYYDPSKIVL 227

Query: 324 DPYAKAVISRGEYGALGPEGNCWPPMAGMVPTSDNRFDWEGDLPLKYPQKDLIIYEMHVR 145
           DPYAK+VISRGE+G LG + N WP MA M+PT++N+FDWEGD PLK+PQ+DLIIYEMHVR
Sbjct: 228 DPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKHPQRDLIIYEMHVR 287

Query: 144 GFTRHDSSRTNFPGTYLGVVEKLDHFKELGVNCIELMPCQEYNELEYF 1
           GFT+H+SSRT FPGTYLGVVEKLDH KELGVNCIELMPC E+NELEY+
Sbjct: 288 GFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYY 335