BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia24_contig00018707
         (1464 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004290831.1| PREDICTED: biotin--protein ligase-like [Frag...   323   2e-85
ref|XP_002301010.2| hypothetical protein POPTR_0002s08830g [Popu...   321   5e-85
emb|CBI19072.3| unnamed protein product [Vitis vinifera]              320   8e-85
ref|XP_002285834.1| PREDICTED: biotin--protein ligase [Vitis vin...   320   8e-85
ref|XP_007221780.1| hypothetical protein PRUPE_ppa007504mg [Prun...   320   1e-84
ref|XP_002307419.1| holocarboxylase synthetase 2 family protein ...   319   2e-84
ref|XP_003601098.1| Holocarboxylase synthetase hcs2 [Medicago tr...   316   2e-83
ref|XP_006472717.1| PREDICTED: biotin--protein ligase-like [Citr...   315   3e-83
ref|XP_007018965.1| Holocarboxylase synthase 1 isoform 1 [Theobr...   315   3e-83
ref|XP_006396014.1| hypothetical protein EUTSA_v10004827mg [Eutr...   313   2e-82
ref|XP_006296439.1| hypothetical protein CARUB_v10025619mg [Caps...   313   2e-82
ref|XP_004500594.1| PREDICTED: biotin--protein ligase-like [Cice...   312   2e-82
ref|XP_006434124.1| hypothetical protein CICLE_v10001583mg [Citr...   311   4e-82
ref|NP_850957.1| holocarboxylase synthetase 2 [Arabidopsis thali...   311   6e-82
ref|NP_174903.3| holocarboxylase synthetase 2 [Arabidopsis thali...   311   6e-82
ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis ly...   311   6e-82
ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thalian...   310   1e-81
gb|AAL93108.1|AF414937_1 holocarboxylase synthetase 1 [Arabidops...   310   1e-81
gb|AAL93111.1|AF414940_1 holocarboxylase synthetase hcs2.c [Arab...   309   2e-81
gb|AAL93106.1|AF414935_1 holocarboxylase synthetase 2 [Arabidops...   309   2e-81

>ref|XP_004290831.1| PREDICTED: biotin--protein ligase-like [Fragaria vesca subsp.
           vesca]
          Length = 329

 Score =  323 bits (827), Expect = 2e-85
 Identities = 147/198 (74%), Positives = 182/198 (91%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LA+TEA+KDVC  NGLPY+D++IKWPNDLYLNGLKVGGILCTSTY
Sbjct: 132 MEDGRVVPLIQYVVSLAITEAIKDVCDRNGLPYIDVRIKWPNDLYLNGLKVGGILCTSTY 191

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           +SKKFN+SAG+GLN++NEKPTTCLNAVL EL++  ++ RREEILAAFF +FE F+D+F++
Sbjct: 192 KSKKFNVSAGVGLNINNEKPTTCLNAVLRELSATTYQFRREEILAAFFNKFEKFYDLFMH 251

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGFQ+LEE YYK WLHSGQRV++QEK + Q+VE+V VT+QGLTS+GYLLA+G+DN++ EL
Sbjct: 252 QGFQSLEELYYKTWLHSGQRVIVQEKNDDQVVENV-VTVQGLTSAGYLLAIGDDNQMCEL 310

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVR+KL
Sbjct: 311 HPDGNSFDFFKGLVRQKL 328



 Score =  125 bits (313), Expect = 6e-26
 Identities = 69/127 (54%), Positives = 85/127 (66%)
 Frame = -2

Query: 1328 SSRLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLHSSETEKPCDFEESFKMELYM 1149
            S  L+L GKS  E   A++LKN + LKL + D  +SI L  SE+EKP    E+F ++ +M
Sbjct: 6    SCMLVLRGKSPAENKIAEALKNTKALKLSD-DTELSILL-DSESEKPFKEGEAFGVDSFM 63

Query: 1148 NNLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXXXXXXXRWKMGA 969
             +LSTDRFGRFL+WSPRL STQD+VSQNFCELP GAVCV DVQFKGR           G 
Sbjct: 64   KSLSTDRFGRFLLWSPRLPSTQDVVSQNFCELPNGAVCVADVQFKGRGRSKNVWESPKGC 123

Query: 968  LCLSYNM 948
            L  S+ +
Sbjct: 124  LLFSFTL 130


>ref|XP_002301010.2| hypothetical protein POPTR_0002s08830g [Populus trichocarpa]
           gi|550344587|gb|EEE80283.2| hypothetical protein
           POPTR_0002s08830g [Populus trichocarpa]
          Length = 390

 Score =  321 bits (823), Expect = 5e-85
 Identities = 155/199 (77%), Positives = 178/199 (89%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPLLQYVV LAVTEA+KDVC  NGLP +D++IKWPNDLYLNGLKVGGIL TSTY
Sbjct: 193 MEDGRVVPLLQYVVSLAVTEAIKDVCDKNGLPRIDVRIKWPNDLYLNGLKVGGILSTSTY 252

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           +SKKFN+S GIGLNVDNEKPTTCLNAVL EL++ A  LRRE+I+AAF  +FENF+D+F+N
Sbjct: 253 KSKKFNVSTGIGLNVDNEKPTTCLNAVLRELSAAACTLRREDIVAAFLNKFENFYDLFIN 312

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
            GF+ LEE YYK WLHSGQRV+IQEK E Q+VE+V VTIQGLTSSGYLLA+GEDN++ EL
Sbjct: 313 GGFRTLEELYYKTWLHSGQRVIIQEKNENQVVENV-VTIQGLTSSGYLLAIGEDNQMCEL 371

Query: 444 HPDGNSLDFFKGLVRRKLE 388
           HPDGNS DFFKGLVRRKLE
Sbjct: 372 HPDGNSFDFFKGLVRRKLE 390



 Score =  122 bits (307), Expect = 3e-25
 Identities = 84/173 (48%), Positives = 104/173 (60%), Gaps = 3/173 (1%)
 Frame = -2

Query: 1460 SPQYSLLLASLSSI--PFHKKSRHFLLSPLKPYLFHPFYNKIMETPSSRLILCGKSSLEI 1287
            SPQ SL L SL +   P  +KS  F LS  +        +    TP  +L+LCGKSS E 
Sbjct: 23   SPQSSLSLRSLHNFIAPTSQKSP-FTLS--RSVAAATAMDSSSSTPC-KLVLCGKSSAEN 78

Query: 1286 DFAKSLKNNEVLKLPEHDVGVSIFLHSSETEKPC-DFEESFKMELYMNNLSTDRFGRFLM 1110
            + AKSL NN  LKLP++ V VS  LHS    K     EESF +E +MN+LST++FGR L+
Sbjct: 79   EIAKSLMNNNTLKLPDN-VQVSTLLHSEIINKQQRQDEESFCIERFMNSLSTNQFGRLLI 137

Query: 1109 WSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXXXXXXXRWKMGALCLSYN 951
            WSP L ST D+VS NF ELPIG VCV DVQ+KGR        W+  A CL ++
Sbjct: 138  WSPILPSTHDVVSNNFGELPIGTVCVADVQYKGRGRSKNV--WESPAGCLMFS 188


>emb|CBI19072.3| unnamed protein product [Vitis vinifera]
          Length = 416

 Score =  320 bits (821), Expect = 8e-85
 Identities = 151/199 (75%), Positives = 178/199 (89%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR VPLLQYVV LA+TEA+K +C + GLP+LD+KIKWPNDLYLNGLKVGGILCTSTY
Sbjct: 219 MEDGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPNDLYLNGLKVGGILCTSTY 278

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           +S+KFN+SAGIGLNVDNEKPTTCLNAVL EL+SVA++ RRE+I+AAFF +FE FF +F+N
Sbjct: 279 KSRKFNVSAGIGLNVDNEKPTTCLNAVLRELSSVAYQFRREDIVAAFFNKFETFFSLFIN 338

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGFQ LEE Y K WLHSGQRV++QE  EGQ+VE+V VTIQGL SSGYLLA+G+D ++ EL
Sbjct: 339 QGFQTLEELYCKTWLHSGQRVIVQENIEGQMVENV-VTIQGLASSGYLLAIGDDGQMCEL 397

Query: 444 HPDGNSLDFFKGLVRRKLE 388
           HPDGNS DFFKGL+RRKLE
Sbjct: 398 HPDGNSFDFFKGLIRRKLE 416



 Score =  134 bits (336), Expect = 1e-28
 Identities = 73/127 (57%), Positives = 89/127 (70%)
 Frame = -2

Query: 1328 SSRLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLHSSETEKPCDFEESFKMELYM 1149
            +S L+LCGKSS EI+FAKSLK    LKLP++   VSI LH  E EKP   EE F+M+L+M
Sbjct: 95   TSLLVLCGKSSAEIEFAKSLKAKNTLKLPDNGE-VSILLHP-EIEKP--EEEPFQMDLFM 150

Query: 1148 NNLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXXXXXXXRWKMGA 969
            ++LST+ FGRFL+WSP+L ST D+VSQNFCELPIG VCV DVQ KGR           G 
Sbjct: 151  SSLSTNTFGRFLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSKNKWESPRGC 210

Query: 968  LCLSYNM 948
            L  S+ +
Sbjct: 211  LLFSFTL 217


>ref|XP_002285834.1| PREDICTED: biotin--protein ligase [Vitis vinifera]
          Length = 327

 Score =  320 bits (821), Expect = 8e-85
 Identities = 151/199 (75%), Positives = 178/199 (89%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR VPLLQYVV LA+TEA+K +C + GLP+LD+KIKWPNDLYLNGLKVGGILCTSTY
Sbjct: 130 MEDGRTVPLLQYVVSLAITEAIKSICDSKGLPHLDVKIKWPNDLYLNGLKVGGILCTSTY 189

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           +S+KFN+SAGIGLNVDNEKPTTCLNAVL EL+SVA++ RRE+I+AAFF +FE FF +F+N
Sbjct: 190 KSRKFNVSAGIGLNVDNEKPTTCLNAVLRELSSVAYQFRREDIVAAFFNKFETFFSLFIN 249

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGFQ LEE Y K WLHSGQRV++QE  EGQ+VE+V VTIQGL SSGYLLA+G+D ++ EL
Sbjct: 250 QGFQTLEELYCKTWLHSGQRVIVQENIEGQMVENV-VTIQGLASSGYLLAIGDDGQMCEL 308

Query: 444 HPDGNSLDFFKGLVRRKLE 388
           HPDGNS DFFKGL+RRKLE
Sbjct: 309 HPDGNSFDFFKGLIRRKLE 327



 Score =  134 bits (336), Expect = 1e-28
 Identities = 73/127 (57%), Positives = 89/127 (70%)
 Frame = -2

Query: 1328 SSRLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLHSSETEKPCDFEESFKMELYM 1149
            +S L+LCGKSS EI+FAKSLK    LKLP++   VSI LH  E EKP   EE F+M+L+M
Sbjct: 6    TSLLVLCGKSSAEIEFAKSLKAKNTLKLPDNGE-VSILLHP-EIEKP--EEEPFQMDLFM 61

Query: 1148 NNLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXXXXXXXRWKMGA 969
            ++LST+ FGRFL+WSP+L ST D+VSQNFCELPIG VCV DVQ KGR           G 
Sbjct: 62   SSLSTNTFGRFLIWSPQLPSTHDVVSQNFCELPIGTVCVADVQLKGRGRSKNKWESPRGC 121

Query: 968  LCLSYNM 948
            L  S+ +
Sbjct: 122  LLFSFTL 128


>ref|XP_007221780.1| hypothetical protein PRUPE_ppa007504mg [Prunus persica]
           gi|462418716|gb|EMJ22979.1| hypothetical protein
           PRUPE_ppa007504mg [Prunus persica]
          Length = 365

 Score =  320 bits (820), Expect = 1e-84
 Identities = 149/199 (74%), Positives = 180/199 (90%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LAVTEA+KDVC  NGLPY+D+KIKWPNDLYLNGLKVGGILCTST+
Sbjct: 168 MEDGRVVPLIQYVVSLAVTEAIKDVCDKNGLPYVDVKIKWPNDLYLNGLKVGGILCTSTF 227

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           +SK+FN+SAGIGLNVDNEKPTTCLN  L EL+   ++ RRE+ILAAFF +FE F+++F+N
Sbjct: 228 KSKQFNVSAGIGLNVDNEKPTTCLNTFLRELSVTTYQFRREDILAAFFDKFEKFYELFIN 287

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGFQ+LEE YYK WLHSGQRV++QEK + Q+VE+V VTIQGLTSSGYLLA+G+DN++ EL
Sbjct: 288 QGFQSLEELYYKTWLHSGQRVIVQEKNDDQVVENV-VTIQGLTSSGYLLAIGDDNQMCEL 346

Query: 444 HPDGNSLDFFKGLVRRKLE 388
           HPDGNS DFFKGLVR+KL+
Sbjct: 347 HPDGNSFDFFKGLVRQKLD 365



 Score =  130 bits (328), Expect = 1e-27
 Identities = 73/138 (52%), Positives = 95/138 (68%), Gaps = 1/138 (0%)
 Frame = -2

Query: 1358 PFYNKIMETPSS-RLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLHSSETEKPCD 1182
            PF    M+  SS  L+LCGKSS E + AK+LKN+  LKL ++   +SI LHS E+ KP  
Sbjct: 32   PFAVSAMDGNSSCALVLCGKSSAENETAKTLKNSGALKLTDN-TELSILLHS-ESAKPIK 89

Query: 1181 FEESFKMELYMNNLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXX 1002
             E++F ++ +MN+LST+RFGRFL+WSPRL ST D++SQNFCELPIGAVCV DVQ+KGR  
Sbjct: 90   -EDAFGVDSFMNSLSTNRFGRFLLWSPRLPSTHDVISQNFCELPIGAVCVADVQYKGRGR 148

Query: 1001 XXXXXRWKMGALCLSYNM 948
                     G L  S+ +
Sbjct: 149  SKNVWESPKGCLLFSFTL 166


>ref|XP_002307419.1| holocarboxylase synthetase 2 family protein [Populus trichocarpa]
           gi|222856868|gb|EEE94415.1| holocarboxylase synthetase 2
           family protein [Populus trichocarpa]
          Length = 331

 Score =  319 bits (818), Expect = 2e-84
 Identities = 153/199 (76%), Positives = 176/199 (88%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+G++VPLLQYVV LAVTEA+KD+C  NGLP++D++IKWPNDLYLNG+KVGGILCTSTY
Sbjct: 134 MEDGQVVPLLQYVVSLAVTEAIKDLCDKNGLPHIDVRIKWPNDLYLNGVKVGGILCTSTY 193

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           RSKKFNISAGIGLNVDNEKPTTCLNAVL EL   A  LRRE+I+AAF  +FE  +D+F+N
Sbjct: 194 RSKKFNISAGIGLNVDNEKPTTCLNAVLRELAGAACALRREDIVAAFLNKFEKLYDLFIN 253

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
            GFQ LEE YYK WLHSGQRV+IQEK E Q+VE+V VTIQGLT SGYLLA+GEDN++ EL
Sbjct: 254 GGFQTLEELYYKTWLHSGQRVIIQEKNENQVVENV-VTIQGLTPSGYLLAIGEDNQMCEL 312

Query: 444 HPDGNSLDFFKGLVRRKLE 388
           HPDGNS DFFKGLVRRK+E
Sbjct: 313 HPDGNSFDFFKGLVRRKIE 331



 Score =  124 bits (310), Expect = 1e-25
 Identities = 65/124 (52%), Positives = 83/124 (66%)
 Frame = -2

Query: 1322 RLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLHSSETEKPCDFEESFKMELYMNN 1143
            +L+LCGKSS E + AKSL NN  LKLP++ V +S   HS   ++P   EESF +  +MN+
Sbjct: 9    KLLLCGKSSAENEIAKSLMNNNTLKLPDN-VEISTLSHSEILDEPQQNEESFSLSRFMNS 67

Query: 1142 LSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXXXXXXXRWKMGALC 963
            LST+ FGR L+WSP L ST DLVS NF ELPIG VC+ DVQ+KGR        W+  A C
Sbjct: 68   LSTNLFGRLLIWSPLLPSTHDLVSNNFGELPIGTVCIADVQYKGRGRSKNV--WESPAGC 125

Query: 962  LSYN 951
            L ++
Sbjct: 126  LMFS 129


>ref|XP_003601098.1| Holocarboxylase synthetase hcs2 [Medicago truncatula]
           gi|355490146|gb|AES71349.1| Holocarboxylase synthetase
           hcs2 [Medicago truncatula]
          Length = 361

 Score =  316 bits (809), Expect = 2e-83
 Identities = 152/199 (76%), Positives = 176/199 (88%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GRIVPL+QYVV LA+TEA+KD+C  NGLP +D+KIKWPNDLYLNG KVGGILCTSTY
Sbjct: 164 MEDGRIVPLVQYVVSLAITEAIKDICDKNGLPCVDVKIKWPNDLYLNGFKVGGILCTSTY 223

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           RSKKFNISAGIGLNV+NEKPTT LN VL EL+  A++ +RE++LAAFF +FE FFD+FLN
Sbjct: 224 RSKKFNISAGIGLNVNNEKPTTSLNTVLRELSVGAYQFQREDVLAAFFNKFEKFFDLFLN 283

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGFQ LEE YYK WLHSGQRV++QEK E ++VE   VTIQGLTSSGYLLAVG+DN++ EL
Sbjct: 284 QGFQTLEELYYKTWLHSGQRVIVQEKNEEKVVEHA-VTIQGLTSSGYLLAVGDDNQMCEL 342

Query: 444 HPDGNSLDFFKGLVRRKLE 388
           HPDGNS DFFKGLVRRKL+
Sbjct: 343 HPDGNSFDFFKGLVRRKLD 361



 Score =  112 bits (281), Expect = 3e-22
 Identities = 60/126 (47%), Positives = 80/126 (63%)
 Frame = -2

Query: 1325 SRLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLHSSETEKPCDFEESFKMELYMN 1146
            S L+LCGKS  E + A ++K N  LKLP+    +SI LHS E  K     +SF+   +MN
Sbjct: 39   SLLVLCGKSLAENETAIAIKTNNTLKLPDKKE-LSIILHS-EFNKSVIQRQSFQFSSFMN 96

Query: 1145 NLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXXXXXXXRWKMGAL 966
            +LST++FGR L+WSP L+ST D+VS NFCELP+G VCV D+Q KGR          +G L
Sbjct: 97   SLSTNQFGRLLIWSPELTSTHDVVSHNFCELPVGTVCVGDIQNKGRGRSKNAWESPLGCL 156

Query: 965  CLSYNM 948
              S+ +
Sbjct: 157  MFSFTL 162


>ref|XP_006472717.1| PREDICTED: biotin--protein ligase-like [Citrus sinensis]
          Length = 366

 Score =  315 bits (808), Expect = 3e-83
 Identities = 148/199 (74%), Positives = 179/199 (89%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPLLQYV  LA+TEA+KDVC  +GLP LDIKIKWPNDLYLNG+KVGGILCTSTY
Sbjct: 169 MEDGRVVPLLQYVASLALTEAIKDVCSRDGLPCLDIKIKWPNDLYLNGIKVGGILCTSTY 228

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           R+KKFN+S GIGLNV+NE+PTTCLNAVL +L+   ++ +RE+++AAFF +FE F+D F+N
Sbjct: 229 RTKKFNVSIGIGLNVNNEEPTTCLNAVLRKLSDSTYQFQREDVIAAFFNKFETFYDTFIN 288

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGFQ+LEE YYK WLHSGQRV+IQEK E Q+VE+V VTIQGLTSSGYLLA+G+DN++ EL
Sbjct: 289 QGFQSLEELYYKTWLHSGQRVIIQEKNEDQVVENV-VTIQGLTSSGYLLAMGDDNQMCEL 347

Query: 444 HPDGNSLDFFKGLVRRKLE 388
           HPDGNSLDFFKGL+RRKL+
Sbjct: 348 HPDGNSLDFFKGLIRRKLQ 366



 Score =  122 bits (305), Expect = 5e-25
 Identities = 69/157 (43%), Positives = 93/157 (59%)
 Frame = -2

Query: 1418 PFHKKSRHFLLSPLKPYLFHPFYNKIMETPSSRLILCGKSSLEIDFAKSLKNNEVLKLPE 1239
            P+ +  R  + +  K +        +    S  L+L GKS  E + A+SLK+N  LKLP+
Sbjct: 17   PYSRSIRSLVSANTKRFRLSASSAAMDSNSSCVLVLSGKSLAENEIAESLKSNSTLKLPD 76

Query: 1238 HDVGVSIFLHSSETEKPCDFEESFKMELYMNNLSTDRFGRFLMWSPRLSSTQDLVSQNFC 1059
            +   VSI L S   +     +ESF +EL+MN+L+T+RFGR L+WSPRL ST D+VS NFC
Sbjct: 77   NSE-VSIHLQSEIVK-----QESFDVELFMNSLATNRFGRLLIWSPRLPSTHDVVSHNFC 130

Query: 1058 ELPIGAVCVTDVQFKGRXXXXXXXRWKMGALCLSYNM 948
            ELP+GAVCV DVQFKGR           G L  S+ +
Sbjct: 131  ELPVGAVCVADVQFKGRGRSKNAWESPKGCLMFSFTI 167


>ref|XP_007018965.1| Holocarboxylase synthase 1 isoform 1 [Theobroma cacao]
           gi|508724293|gb|EOY16190.1| Holocarboxylase synthase 1
           isoform 1 [Theobroma cacao]
          Length = 375

 Score =  315 bits (808), Expect = 3e-83
 Identities = 147/199 (73%), Positives = 178/199 (89%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+G+IVPL+QYVV LA+TEA+KD+C   G P LD++IKWPNDLYLNGLKVGGILCTSTY
Sbjct: 178 MEDGKIVPLVQYVVSLALTEAIKDICDREGFPCLDVRIKWPNDLYLNGLKVGGILCTSTY 237

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           +SKKFN+S+G+GLNVDNEKPTTCLNAVL EL++ ++K RRE+I+AAFF +FE  +D+FLN
Sbjct: 238 KSKKFNVSSGVGLNVDNEKPTTCLNAVLKELSTTSNKFRREDIMAAFFNKFEMLYDIFLN 297

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGFQ LEE YY+ WLHSGQRV++QEK E ++VE+V VTIQGLTSSGYLLA+G+D ++ EL
Sbjct: 298 QGFQTLEELYYRTWLHSGQRVIVQEKNEDRIVENV-VTIQGLTSSGYLLAIGDDYQMCEL 356

Query: 444 HPDGNSLDFFKGLVRRKLE 388
           HPDGNS DFFKGLVRRKLE
Sbjct: 357 HPDGNSFDFFKGLVRRKLE 375



 Score =  122 bits (306), Expect = 4e-25
 Identities = 71/133 (53%), Positives = 83/133 (62%), Gaps = 2/133 (1%)
 Frame = -2

Query: 1340 METPS-SRLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLH-SSETEKPCDFEESF 1167
            MET S S L LCGKSS E + AKSLK N  LKLP    G  +  H  SE       EE+F
Sbjct: 47   METSSPSALFLCGKSSSETEIAKSLKINNALKLPN---GGEVSTHLQSEISNFQLGEEAF 103

Query: 1166 KMELYMNNLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXXXXXXX 987
             + L++N+LST+RFGR L+WSPRL ST D+VS NFCELPIGA CV DVQFKGR       
Sbjct: 104  NVNLFLNSLSTNRFGRNLIWSPRLPSTHDVVSHNFCELPIGAACVADVQFKGRGRSKNAW 163

Query: 986  RWKMGALCLSYNM 948
                G L  S+ +
Sbjct: 164  ESPRGCLMFSFTL 176


>ref|XP_006396014.1| hypothetical protein EUTSA_v10004827mg [Eutrema salsugineum]
           gi|557092653|gb|ESQ33300.1| hypothetical protein
           EUTSA_v10004827mg [Eutrema salsugineum]
          Length = 247

 Score =  313 bits (801), Expect = 2e-82
 Identities = 144/198 (72%), Positives = 178/198 (89%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GRIVPL+QYVV LAVTEAVKDVCH  GLPY+D+KIKWPNDLYLNGLKVGGILCTSTY
Sbjct: 51  MEDGRIVPLIQYVVSLAVTEAVKDVCHKKGLPYIDVKIKWPNDLYLNGLKVGGILCTSTY 110

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           RS+KF++  G+GLNVDN++PTTCLNAVL +++  ++ L+REEIL AFF +FE FFD+F++
Sbjct: 111 RSRKFHVCVGVGLNVDNKQPTTCLNAVLEDMSPASNLLKREEILGAFFHKFEKFFDLFMD 170

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGF +LEE YYK WLHS QRV+++EK E ++V+++ VTIQGLTSSGYLLA+G+DN++YEL
Sbjct: 171 QGFNSLEELYYKKWLHSEQRVIVEEKIEDRVVQNI-VTIQGLTSSGYLLAIGDDNQMYEL 229

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVRRK+
Sbjct: 230 HPDGNSFDFFKGLVRRKI 247


>ref|XP_006296439.1| hypothetical protein CARUB_v10025619mg [Capsella rubella]
           gi|482565147|gb|EOA29337.1| hypothetical protein
           CARUB_v10025619mg [Capsella rubella]
          Length = 357

 Score =  313 bits (801), Expect = 2e-82
 Identities = 146/198 (73%), Positives = 177/198 (89%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LAVTEAVKDVC   GLPY+D+KIKWPNDLYLNGLKVGGILCTSTY
Sbjct: 161 MEDGRVVPLVQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYLNGLKVGGILCTSTY 220

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           RSKKF++S G+GLNVDNE+PTTCLNA L E++  ++ L REEIL AFF +FE FFD+F++
Sbjct: 221 RSKKFHVSVGVGLNVDNEQPTTCLNAGLKEMSPASNLLNREEILGAFFHKFEKFFDLFMD 280

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGF++LEE YY+ WLHS QRV+++EK E Q+V++V VTIQGLTSSGYLLA+G+DN++YEL
Sbjct: 281 QGFKSLEELYYRTWLHSEQRVIVEEKVEDQVVQNV-VTIQGLTSSGYLLAIGDDNQMYEL 339

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVRRK+
Sbjct: 340 HPDGNSFDFFKGLVRRKI 357



 Score =  127 bits (319), Expect = 1e-26
 Identities = 67/148 (45%), Positives = 91/148 (61%), Gaps = 1/148 (0%)
 Frame = -2

Query: 1388 LSPLKPYLFHPFYNKIMETPSSRLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLH 1209
            L  L+P  F    + +    S  L+LCGKSS+E + AK LK+   LK P+ +  VSIFL 
Sbjct: 12   LKLLRPLSFSVAASAMESDASCSLVLCGKSSVETEIAKRLKDKNSLKFPDDNTKVSIFLE 71

Query: 1208 SSETEKPCDFEESF-KMELYMNNLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCV 1032
            S       D ++ F  + L+MN++ T +FGRFL+WSPRLSST D+VS NF ELP+G+VCV
Sbjct: 72   SEAKHLVKDDDDGFFNLSLFMNSIITHQFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCV 131

Query: 1031 TDVQFKGRXXXXXXXRWKMGALCLSYNM 948
            TD+QFKGR           G L  S+++
Sbjct: 132  TDIQFKGRGRTKNVWESPKGCLMYSFSL 159


>ref|XP_004500594.1| PREDICTED: biotin--protein ligase-like [Cicer arietinum]
          Length = 347

 Score =  312 bits (800), Expect = 2e-82
 Identities = 149/198 (75%), Positives = 175/198 (88%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LA+TEA+KD+C  NGLP +D+KIKWPNDLYLNG KVGGILCTSTY
Sbjct: 150 MEDGRVVPLVQYVVSLAITEAIKDICDKNGLPCIDVKIKWPNDLYLNGFKVGGILCTSTY 209

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           +SKKFNISAGIGLNV NEKPTT LN VL EL+  A++ +RE++LAAFF +FE FFD+F+N
Sbjct: 210 KSKKFNISAGIGLNVHNEKPTTSLNTVLRELSVGAYQFQREDVLAAFFNKFEIFFDLFVN 269

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGFQ LEE YYK WLHSGQRVV+QEK E ++VE V VT+QGLTSSGYLLA+G+DN++ EL
Sbjct: 270 QGFQTLEELYYKTWLHSGQRVVVQEKNEDKVVEHV-VTVQGLTSSGYLLAIGDDNQMCEL 328

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVRRKL
Sbjct: 329 HPDGNSFDFFKGLVRRKL 346



 Score =  115 bits (288), Expect = 5e-23
 Identities = 60/124 (48%), Positives = 84/124 (67%)
 Frame = -2

Query: 1319 LILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLHSSETEKPCDFEESFKMELYMNNL 1140
            L+LCGKS+ EI+ A ++K N  LKLP+    +S+ LHS E  K  + + SF++  +MN+L
Sbjct: 28   LVLCGKSNAEIETAIAIKANNTLKLPDKGE-LSLVLHS-ELNKSVE-QNSFQVSSFMNSL 84

Query: 1139 STDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXXXXXXXRWKMGALCL 960
            ST++FGR L+WSPRL+ST D+VS NFCELP+G VCV D+Q KGR          +G L  
Sbjct: 85   STNQFGRLLIWSPRLTSTHDVVSNNFCELPLGTVCVADIQNKGRGRSKNVWESPLGCLMF 144

Query: 959  SYNM 948
            S+ +
Sbjct: 145  SFTL 148


>ref|XP_006434124.1| hypothetical protein CICLE_v10001583mg [Citrus clementina]
           gi|557536246|gb|ESR47364.1| hypothetical protein
           CICLE_v10001583mg [Citrus clementina]
          Length = 366

 Score =  311 bits (798), Expect = 4e-82
 Identities = 146/199 (73%), Positives = 176/199 (88%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPLLQYV  LA+TEA+  VC  +GLP LDIKIKWPNDLYLNG+KVGGILCTSTY
Sbjct: 169 MEDGRVVPLLQYVASLALTEAINYVCSRDGLPCLDIKIKWPNDLYLNGIKVGGILCTSTY 228

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           R+KKFN+S GIGLNV+NE+PTTCLNAVL +L+   ++ RRE+++AAFF +FE F+D F+N
Sbjct: 229 RTKKFNVSIGIGLNVNNEEPTTCLNAVLRKLSDSTYQFRREDVIAAFFNKFETFYDTFIN 288

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGFQ LEE YYK WLHSGQRV++QEK E Q+VE+V VTIQGLTSSGYLLA+G+DN++ EL
Sbjct: 289 QGFQTLEELYYKTWLHSGQRVIVQEKNEDQVVENV-VTIQGLTSSGYLLAIGDDNQMCEL 347

Query: 444 HPDGNSLDFFKGLVRRKLE 388
           HPDGNSLDFFKGL+RRKL+
Sbjct: 348 HPDGNSLDFFKGLIRRKLQ 366



 Score =  123 bits (308), Expect = 2e-25
 Identities = 70/157 (44%), Positives = 92/157 (58%)
 Frame = -2

Query: 1418 PFHKKSRHFLLSPLKPYLFHPFYNKIMETPSSRLILCGKSSLEIDFAKSLKNNEVLKLPE 1239
            P     R  + +  KP+        +    S  L+L GKS  E + A+SLK+N  LKLP+
Sbjct: 17   PSSPSIRSLVSANTKPFRLSASSAAMDSNSSCMLVLSGKSLAENEIAESLKSNSTLKLPD 76

Query: 1238 HDVGVSIFLHSSETEKPCDFEESFKMELYMNNLSTDRFGRFLMWSPRLSSTQDLVSQNFC 1059
            +   VSI L S   +     +ESF +EL+MN+L+T+RFGR L+WSPRL ST D+VS NFC
Sbjct: 77   NSE-VSIHLQSEIVK-----QESFDVELFMNSLATNRFGRLLIWSPRLPSTHDVVSHNFC 130

Query: 1058 ELPIGAVCVTDVQFKGRXXXXXXXRWKMGALCLSYNM 948
            ELP+GAVCV DVQFKGR           G L  S+ +
Sbjct: 131  ELPVGAVCVADVQFKGRGRSKNAWESPKGCLMFSFTI 167


>ref|NP_850957.1| holocarboxylase synthetase 2 [Arabidopsis thaliana]
           gi|614084158|sp|F4I4W2.1|HCS2_ARATH RecName:
           Full=Biotin--protein ligase 2; AltName:
           Full=Holocarboxylase synthetase 2
           gi|332193776|gb|AEE31897.1| holocarboxylase synthetase 2
           [Arabidopsis thaliana]
          Length = 329

 Score =  311 bits (796), Expect = 6e-82
 Identities = 147/198 (74%), Positives = 175/198 (88%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LAVTEAVKDVC   GL Y D+KIKWPNDLYLNGLK+GGILCTSTY
Sbjct: 133 MEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNGLKIGGILCTSTY 192

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           RS+KF +S G+GLNVDNE+PTTCLNAVL ++   ++ L+REEIL AFFK+FENFFD+F+ 
Sbjct: 193 RSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFKKFENFFDLFME 252

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGF++LEE YY+ WLHSGQRV+ +EK E Q+V++V VTIQGLTSSGYLLA+G+DN +YEL
Sbjct: 253 QGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNV-VTIQGLTSSGYLLAIGDDNVMYEL 311

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVRRKL
Sbjct: 312 HPDGNSFDFFKGLVRRKL 329



 Score =  123 bits (309), Expect = 2e-25
 Identities = 66/126 (52%), Positives = 84/126 (66%)
 Frame = -2

Query: 1328 SSRLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLHSSETEKPCDFEESFKMELYM 1149
            S  L+L GKSS+E D A  LKNN VLKLP++   VSIFL S       D + SF + L+M
Sbjct: 6    SCSLVLYGKSSVETDTATRLKNNNVLKLPDNSK-VSIFLQSEIKNLVRDDDSSFNLSLFM 64

Query: 1148 NNLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXXXXXXXRWKMGA 969
            N++ST RFGRFL+WSP LSST D+VS NF E+P+G+VCV+D+Q KGR        W+   
Sbjct: 65   NSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNV--WESPK 122

Query: 968  LCLSYN 951
             CL Y+
Sbjct: 123  GCLMYS 128


>ref|NP_174903.3| holocarboxylase synthetase 2 [Arabidopsis thaliana]
           gi|332193774|gb|AEE31895.1| holocarboxylase synthetase 2
           [Arabidopsis thaliana]
          Length = 286

 Score =  311 bits (796), Expect = 6e-82
 Identities = 147/198 (74%), Positives = 175/198 (88%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LAVTEAVKDVC   GL Y D+KIKWPNDLYLNGLK+GGILCTSTY
Sbjct: 90  MEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNGLKIGGILCTSTY 149

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           RS+KF +S G+GLNVDNE+PTTCLNAVL ++   ++ L+REEIL AFFK+FENFFD+F+ 
Sbjct: 150 RSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFKKFENFFDLFME 209

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGF++LEE YY+ WLHSGQRV+ +EK E Q+V++V VTIQGLTSSGYLLA+G+DN +YEL
Sbjct: 210 QGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQNV-VTIQGLTSSGYLLAIGDDNVMYEL 268

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVRRKL
Sbjct: 269 HPDGNSFDFFKGLVRRKL 286


>ref|XP_002893926.1| holocarboxylase synthetase 2 [Arabidopsis lyrata subsp. lyrata]
           gi|297339768|gb|EFH70185.1| holocarboxylase synthetase 2
           [Arabidopsis lyrata subsp. lyrata]
          Length = 328

 Score =  311 bits (796), Expect = 6e-82
 Identities = 147/198 (74%), Positives = 174/198 (87%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LAVTEAVKDVC   GL Y+D+KIKWPNDLYLNGLKVGGILCTSTY
Sbjct: 132 MEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYIDVKIKWPNDLYLNGLKVGGILCTSTY 191

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
            S+KF++S G+GLNVDNEKPTTCLNAVL E    ++ L+REEIL AFF +FE FFD+F+ 
Sbjct: 192 GSRKFHVSVGVGLNVDNEKPTTCLNAVLKENCPASNLLKREEILGAFFHKFETFFDLFME 251

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGF++LEE YY+ WLHSGQRV+++EK E Q+V++V VTIQGLTSSGYLLA+G+DN +YEL
Sbjct: 252 QGFKSLEELYYRTWLHSGQRVIVEEKNEDQVVQNV-VTIQGLTSSGYLLAIGDDNVMYEL 310

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVRRKL
Sbjct: 311 HPDGNSFDFFKGLVRRKL 328



 Score =  129 bits (324), Expect = 3e-27
 Identities = 69/126 (54%), Positives = 88/126 (69%)
 Frame = -2

Query: 1328 SSRLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLHSSETEKPCDFEESFKMELYM 1149
            S  L+LCGKSS+E D A  LKNN VLKLP++   VSIFL S E +     ++SF + L+M
Sbjct: 6    SCSLVLCGKSSVETDAATRLKNNNVLKLPDN-TKVSIFLQS-EIKNLVKDDDSFNLSLFM 63

Query: 1148 NNLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVTDVQFKGRXXXXXXXRWKMGA 969
            N++ST RFGRFL+WSP LSST D+VS NF E+P+G+VCVTD+QFKGR        W+   
Sbjct: 64   NSISTHRFGRFLIWSPFLSSTHDVVSHNFSEIPVGSVCVTDIQFKGRGRTKNV--WESPK 121

Query: 968  LCLSYN 951
             CL Y+
Sbjct: 122  GCLMYS 127


>ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thaliana]
           gi|30682866|ref|NP_850067.1| holocarboxylase synthase 1
           [Arabidopsis thaliana]
           gi|75206635|sp|Q9SL92.2|HCS1_ARATH RecName:
           Full=Biotin--protein ligase 1, chloroplastic; AltName:
           Full=Holocarboxylase synthetase 1; Includes: RecName:
           Full=Biotin--[methylcrotonoyl-CoA-carboxylase] ligase;
           Includes: RecName: Full=Biotin--[acetyl-CoA-carboxylase]
           ligase; Flags: Precursor gi|1688072|gb|AAC49706.1|
           biotin holocarboxylase synthetase [Arabidopsis thaliana]
           gi|20197509|gb|AAD31371.2| biotin holocarboxylase
           synthetase [Arabidopsis thaliana]
           gi|89000993|gb|ABD59086.1| At2g25710 [Arabidopsis
           thaliana] gi|330252646|gb|AEC07740.1| holocarboxylase
           synthase 1 [Arabidopsis thaliana]
           gi|330252647|gb|AEC07741.1| holocarboxylase synthase 1
           [Arabidopsis thaliana]
          Length = 367

 Score =  310 bits (794), Expect = 1e-81
 Identities = 145/198 (73%), Positives = 176/198 (88%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LAVTEAVKDVC   GLPY+D+KIKWPNDLY+NGLKVGGILCTSTY
Sbjct: 171 MEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYVNGLKVGGILCTSTY 230

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           RSKKFN+S G+GLNVDN +PTTCLNAVL  +   ++ L+REEIL AFF +FE FFD+F++
Sbjct: 231 RSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILGAFFHKFEKFFDLFMD 290

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGF++LEE YY+ WLHS QRV++++K E Q+V++V VTIQGLTSSGYLLAVG+DN++YEL
Sbjct: 291 QGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNV-VTIQGLTSSGYLLAVGDDNQMYEL 349

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVRRK+
Sbjct: 350 HPDGNSFDFFKGLVRRKI 367



 Score =  129 bits (325), Expect = 3e-27
 Identities = 74/150 (49%), Positives = 95/150 (63%), Gaps = 3/150 (2%)
 Frame = -2

Query: 1391 LLSPLKPY--LFHPFYNKIMETPSS-RLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVS 1221
            +L  LKP   L   F    ME+ +S  L+LCGKSS+E + AK LKN   LKLP++   VS
Sbjct: 20   VLRSLKPLHRLSFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDN-TKVS 78

Query: 1220 IFLHSSETEKPCDFEESFKMELYMNNLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGA 1041
            + L S       D + SF + L+MN++ T RFGRFL+WSPRLSST D+VS NF ELP+G+
Sbjct: 79   LILESEAKNLVKDDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGS 138

Query: 1040 VCVTDVQFKGRXXXXXXXRWKMGALCLSYN 951
            VCVTD+QFKGR        W+    CL Y+
Sbjct: 139  VCVTDIQFKGRGRTKNV--WESPKGCLMYS 166


>gb|AAL93108.1|AF414937_1 holocarboxylase synthetase 1 [Arabidopsis thaliana]
          Length = 367

 Score =  310 bits (794), Expect = 1e-81
 Identities = 145/198 (73%), Positives = 176/198 (88%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LAVTEAVKDVC   GLPY+D+KIKWPNDLY+NGLKVGGILCTSTY
Sbjct: 171 MEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYVNGLKVGGILCTSTY 230

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           RSKKFN+S G+GLNVDN +PTTCLNAVL  +   ++ L+REEIL AFF +FE FFD+F++
Sbjct: 231 RSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILGAFFHKFEKFFDLFMD 290

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGF++LEE YY+ WLHS QRV++++K E Q+V++V VTIQGLTSSGYLLAVG+DN++YEL
Sbjct: 291 QGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNV-VTIQGLTSSGYLLAVGDDNQMYEL 349

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVRRK+
Sbjct: 350 HPDGNSFDFFKGLVRRKI 367



 Score =  129 bits (325), Expect = 3e-27
 Identities = 70/146 (47%), Positives = 91/146 (62%)
 Frame = -2

Query: 1388 LSPLKPYLFHPFYNKIMETPSSRLILCGKSSLEIDFAKSLKNNEVLKLPEHDVGVSIFLH 1209
            L PL+   F    + +    S  L+LCGKSS+E + AK LKN   LKLP++   VS+ L 
Sbjct: 24   LKPLRRLSFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDN-TKVSLILE 82

Query: 1208 SSETEKPCDFEESFKMELYMNNLSTDRFGRFLMWSPRLSSTQDLVSQNFCELPIGAVCVT 1029
            S       D + SF + L+MN++ T RFGRFL+WSPRLSST D+VS NF ELP+G+VCVT
Sbjct: 83   SEAKNLVKDDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVT 142

Query: 1028 DVQFKGRXXXXXXXRWKMGALCLSYN 951
            D+QFKGR        W+    CL Y+
Sbjct: 143  DIQFKGRGRTKNV--WESPKGCLMYS 166


>gb|AAL93111.1|AF414940_1 holocarboxylase synthetase hcs2.c [Arabidopsis thaliana]
          Length = 286

 Score =  309 bits (792), Expect = 2e-81
 Identities = 145/198 (73%), Positives = 175/198 (88%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LAVTEAVKDVC   GL Y+D+KIKWPNDLYLNGLK+GGILCTSTY
Sbjct: 90  MEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYIDVKIKWPNDLYLNGLKIGGILCTSTY 149

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           RS+KF +S G+GLNVDNE+PTTCLNAVL ++   ++ L+REEIL AFF +FENFFD+F+ 
Sbjct: 150 RSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFNKFENFFDLFME 209

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGF++LEE YY+ WLHSGQ+V+ +EK E Q+V++V VTIQGLTSSGYLLA+G+DN +YEL
Sbjct: 210 QGFKSLEELYYRTWLHSGQKVIAEEKNEDQVVQNV-VTIQGLTSSGYLLAIGDDNIMYEL 268

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVRRKL
Sbjct: 269 HPDGNSFDFFKGLVRRKL 286


>gb|AAL93106.1|AF414935_1 holocarboxylase synthetase 2 [Arabidopsis thaliana]
           gi|19698363|gb|AAL93107.1|AF414936_1 holocarboxylase
           synthetase 2 [Arabidopsis thaliana]
           gi|19698369|gb|AAL93110.1|AF414939_1 holocarboxylase
           synthetase hcs2.b [Arabidopsis thaliana]
          Length = 246

 Score =  309 bits (792), Expect = 2e-81
 Identities = 145/198 (73%), Positives = 175/198 (88%)
 Frame = -1

Query: 984 MENGRIVPLLQYVVCLAVTEAVKDVCHANGLPYLDIKIKWPNDLYLNGLKVGGILCTSTY 805
           ME+GR+VPL+QYVV LAVTEAVKDVC   GL Y+D+KIKWPNDLYLNGLK+GGILCTSTY
Sbjct: 50  MEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYIDVKIKWPNDLYLNGLKIGGILCTSTY 109

Query: 804 RSKKFNISAGIGLNVDNEKPTTCLNAVLGELTSVAHKLRREEILAAFFKRFENFFDVFLN 625
           RS+KF +S G+GLNVDNE+PTTCLNAVL ++   ++ L+REEIL AFF +FENFFD+F+ 
Sbjct: 110 RSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFNKFENFFDLFME 169

Query: 624 QGFQALEEPYYKAWLHSGQRVVIQEKTEGQLVEDVPVTIQGLTSSGYLLAVGEDNKLYEL 445
           QGF++LEE YY+ WLHSGQ+V+ +EK E Q+V++V VTIQGLTSSGYLLA+G+DN +YEL
Sbjct: 170 QGFKSLEELYYRTWLHSGQKVIAEEKNEDQVVQNV-VTIQGLTSSGYLLAIGDDNIMYEL 228

Query: 444 HPDGNSLDFFKGLVRRKL 391
           HPDGNS DFFKGLVRRKL
Sbjct: 229 HPDGNSFDFFKGLVRRKL 246