BLASTX nr result
ID: Akebia23_contig00062421
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Akebia23_contig00062421
(341 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ETS84305.1| hypothetical protein PFICI_02330 [Pestalotiopsis ... 192 3e-47
gb|ERS97297.1| hypothetical protein HMPREF1624_06629 [Sporothrix... 177 1e-42
gb|EOO03312.1| putative carbohydrate esterase family 1 protein [... 176 3e-42
gb|EPE25168.1| alpha/beta-Hydrolase [Glarea lozoyensis ATCC 20868] 160 1e-37
gb|EHL01261.1| putative Acetylxylan esterase A [Glarea lozoyensi... 160 1e-37
ref|XP_007293205.1| carbohydrate esterase family 1 protein [Mars... 144 2e-32
ref|XP_007337284.1| hypothetical protein AURDEDRAFT_179578 [Auri... 137 2e-30
ref|XP_007599692.1| PHB depolymerase family esterase [Colletotri... 136 3e-30
gb|EPS41801.1| hypothetical protein H072_4263 [Dactylellina hapt... 132 5e-29
gb|EPE28446.1| alpha/beta-Hydrolase [Glarea lozoyensis ATCC 20868] 131 8e-29
gb|EHK98423.1| putative Acetylxylan esterase A [Glarea lozoyensi... 131 8e-29
gb|EQB43381.1| PHB depolymerase family esterase [Colletotrichum ... 131 1e-28
gb|EWM63876.1| feruloyl esterase B [Micromonospora sp. M42] 130 2e-28
ref|XP_007279078.1| phb depolymerase family esterase [Colletotri... 129 3e-28
ref|XP_007346063.1| PHB depolymerase family esterase [Auriculari... 129 4e-28
gb|ENH80390.1| feruloyl esterase b precursor [Colletotrichum orb... 129 5e-28
ref|WP_007461339.1| esterase [Micromonospora lupini] gi|38588482... 128 9e-28
ref|WP_020523700.1| hypothetical protein [Catelliglobosispora ko... 127 2e-27
emb|CCA74360.1| probable acetylxylan esterase [Piriformospora in... 127 2e-27
gb|EHK46536.1| carbohydrate esterase family 1 protein [Trichoder... 126 3e-27
>gb|ETS84305.1| hypothetical protein PFICI_02330 [Pestalotiopsis fici W106-1]
Length = 300
Score = 192 bits (489), Expect = 3e-47
Identities = 86/107 (80%), Positives = 94/107 (87%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KTAQQWGD R Y+PGY G PRMQIWHGT+D+TLRYPNYQE +K+WSNVL LT SKDTA
Sbjct: 193 KTAQQWGDLVRSYYPGYTGARPRMQIWHGTADTTLRYPNYQEALKEWSNVLNLTTSKDTA 252
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
N+PQSGYTMTIYG+GTA TAQLVGYSAQGVGHTVP+H DLAFFGI
Sbjct: 253 NSPQSGYTMTIYGEGTATTAQLVGYSAQGVGHTVPVHETMDLAFFGI 299
>gb|ERS97297.1| hypothetical protein HMPREF1624_06629 [Sporothrix schenckii ATCC
58251]
Length = 301
Score = 177 bits (450), Expect = 1e-42
Identities = 79/107 (73%), Positives = 89/107 (83%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KTA QWGD TR Y+ GY G PRMQIWHGT+D+TL YPNYQET+KQWSNVLGL+ K A
Sbjct: 193 KTAAQWGDLTRSYYKGYTGPRPRMQIWHGTADNTLHYPNYQETLKQWSNVLGLSDPKTAA 252
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTPQSGYT TIYG GT+ TAQLVGYS QGVGHTVP+H + D+AFFG+
Sbjct: 253 NTPQSGYTQTIYGAGTSTTAQLVGYSGQGVGHTVPVHESMDIAFFGL 299
>gb|EOO03312.1| putative carbohydrate esterase family 1 protein [Togninia minima
UCRPA7]
Length = 299
Score = 176 bits (446), Expect = 3e-42
Identities = 79/107 (73%), Positives = 91/107 (85%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KTAQQWGD R Y+PGY G PRMQI+HGT+D+TL YPNYQE +K+WSNVL ++ +K
Sbjct: 192 KTAQQWGDLVRSYYPGYTGPRPRMQIFHGTADNTLAYPNYQEQLKEWSNVLNVSLTKTAQ 251
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTPQSGYT ++YGDGTAATAQLVGYSAQGVGHTVPIH + DLAFFGI
Sbjct: 252 NTPQSGYTQSLYGDGTAATAQLVGYSAQGVGHTVPIHQSMDLAFFGI 298
>gb|EPE25168.1| alpha/beta-Hydrolase [Glarea lozoyensis ATCC 20868]
Length = 298
Score = 160 bits (406), Expect = 1e-37
Identities = 73/107 (68%), Positives = 85/107 (79%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KTAQQWGD TR Y+P YNGT P+M IWHGT+D+TL YPN+ E +KQWSNVL + +KD A
Sbjct: 196 KTAQQWGDLTRSYYPNYNGTRPKMLIWHGTADNTLGYPNFAEALKQWSNVLNVPFTKDNA 255
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTPQSGYT IYGDGT +L+GYS QGVGHTVPIH DLA+FG+
Sbjct: 256 NTPQSGYTQKIYGDGT----KLIGYSGQGVGHTVPIHEDVDLAWFGL 298
>gb|EHL01261.1| putative Acetylxylan esterase A [Glarea lozoyensis 74030]
Length = 259
Score = 160 bits (406), Expect = 1e-37
Identities = 73/107 (68%), Positives = 85/107 (79%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KTAQQWGD TR Y+P YNGT P+M IWHGT+D+TL YPN+ E +KQWSNVL + +KD A
Sbjct: 157 KTAQQWGDLTRSYYPNYNGTRPKMLIWHGTADNTLGYPNFAEALKQWSNVLNVPFTKDNA 216
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTPQSGYT IYGDGT +L+GYS QGVGHTVPIH DLA+FG+
Sbjct: 217 NTPQSGYTQKIYGDGT----KLIGYSGQGVGHTVPIHEDVDLAWFGL 259
>ref|XP_007293205.1| carbohydrate esterase family 1 protein [Marssonina brunnea f. sp.
'multigermtubi' MB_m1] gi|406863800|gb|EKD16847.1|
carbohydrate esterase family 1 protein [Marssonina
brunnea f. sp. 'multigermtubi' MB_m1]
Length = 298
Score = 144 bits (362), Expect = 2e-32
Identities = 64/107 (59%), Positives = 81/107 (75%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
K+AQ WGD R Y+PGY G P+M IWHG D+ L YPN ET+KQWSNVL + +K+T
Sbjct: 195 KSAQDWGDLARSYYPGYTGPRPKMMIWHGDQDTILAYPNLAETLKQWSNVLDVAFTKNTT 254
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
+TP+SGYT +YGDGT +L+GYSA+GVGHTVP+H+ DLA+FGI
Sbjct: 255 DTPESGYTEMMYGDGT----KLLGYSAKGVGHTVPVHATVDLAWFGI 297
>ref|XP_007337284.1| hypothetical protein AURDEDRAFT_179578 [Auricularia delicata
TFB-10046 SS5] gi|393246911|gb|EJD54419.1| hypothetical
protein AURDEDRAFT_179578 [Auricularia delicata
TFB-10046 SS5]
Length = 371
Score = 137 bits (344), Expect = 2e-30
Identities = 62/113 (54%), Positives = 83/113 (73%), Gaps = 2/113 (1%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGL--TASKD 168
KTAQ WGD RG +PGY G P++ +WHGT+D+TL+YPN+ + + +W+NV G+ TA++
Sbjct: 188 KTAQAWGDLVRGAYPGYTGPRPKVMLWHGTADTTLQYPNFGQAILEWTNVFGVSSTATQT 247
Query: 167 TANTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGIV*RG 9
+N PQS YT T+YGD A+ ++GYSAQGVGHTVP+H A DL FFGI G
Sbjct: 248 LSNNPQSSYTKTLYGD--ASNPVVIGYSAQGVGHTVPVHEAIDLDFFGITGSG 298
>ref|XP_007599692.1| PHB depolymerase family esterase [Colletotrichum fioriniae PJ7]
gi|588894842|gb|EXF76694.1| PHB depolymerase family
esterase [Colletotrichum fioriniae PJ7]
Length = 292
Score = 136 bits (342), Expect = 3e-30
Identities = 60/107 (56%), Positives = 80/107 (74%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KT +QW Q R +PGYNG++PR+Q +HGT+DS ++YPN E +K+WS + G++ +K+
Sbjct: 190 KTGEQWAAQVRAMYPGYNGSYPRVQTFHGTADSLVKYPNLAEQLKEWSTIHGVSFAKNNT 249
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTP SGYT IYGDGT +LVGYSAQGVGHTVP+ QDL +FG+
Sbjct: 250 NTPISGYTQMIYGDGT----KLVGYSAQGVGHTVPVQEDQDLKWFGL 292
>gb|EPS41801.1| hypothetical protein H072_4263 [Dactylellina haptotyla CBS 200.50]
Length = 305
Score = 132 bits (332), Expect = 5e-29
Identities = 62/107 (57%), Positives = 73/107 (68%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KTAQ+WG+ R +PG+ G PRMQIWHG +D+ + NYQE +KQWSNVLG T +
Sbjct: 124 KTAQEWGNFARNTYPGFTGRRPRMQIWHGGADNLIYPQNYQEELKQWSNVLGQTLTSSNQ 183
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTP SGYT ++YGDGT QLVGY A GVGH VP H L FFGI
Sbjct: 184 NTPDSGYTQSVYGDGT----QLVGYLAAGVGHLVPFHETAVLKFFGI 226
>gb|EPE28446.1| alpha/beta-Hydrolase [Glarea lozoyensis ATCC 20868]
Length = 298
Score = 131 bits (330), Expect = 8e-29
Identities = 62/107 (57%), Positives = 77/107 (71%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KTAQQWGDQ R Y+PGY G+ P+M I+HGT D+TL Y N+ E +K+WSNVLG++ + +
Sbjct: 195 KTAQQWGDQVRSYYPGYTGSRPKMLIFHGTIDTTLYYANFGEELKEWSNVLGVSFNHNQT 254
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTP SGYT IYGDGT +LVG S VGHT P+ DLA+FGI
Sbjct: 255 NTPISGYTKMIYGDGT----KLVGVSCYNVGHTPPVRVPDDLAWFGI 297
>gb|EHK98423.1| putative Acetylxylan esterase A [Glarea lozoyensis 74030]
Length = 261
Score = 131 bits (330), Expect = 8e-29
Identities = 62/107 (57%), Positives = 77/107 (71%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KTAQQWGDQ R Y+PGY G+ P+M I+HGT D+TL Y N+ E +K+WSNVLG++ + +
Sbjct: 158 KTAQQWGDQVRSYYPGYTGSRPKMLIFHGTIDTTLYYANFGEELKEWSNVLGVSFNHNQT 217
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTP SGYT IYGDGT +LVG S VGHT P+ DLA+FGI
Sbjct: 218 NTPISGYTKMIYGDGT----KLVGVSCYNVGHTPPVRVPDDLAWFGI 260
>gb|EQB43381.1| PHB depolymerase family esterase [Colletotrichum gloeosporioides
Cg-14]
Length = 290
Score = 131 bits (329), Expect = 1e-28
Identities = 58/107 (54%), Positives = 77/107 (71%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KT +W Q R FPGYNG++PRMQ +HGT+D+ + YPN E +K+WS + G++ +K+
Sbjct: 188 KTGAEWAAQVRAMFPGYNGSYPRMQTFHGTADTLVTYPNLAEQLKEWSTIHGVSFTKNNT 247
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTP SGYT +YGDGT +LVG SAQGVGHTVP+ QDL +FG+
Sbjct: 248 NTPLSGYTQIVYGDGT----KLVGISAQGVGHTVPVQEDQDLKWFGL 290
>gb|EWM63876.1| feruloyl esterase B [Micromonospora sp. M42]
Length = 454
Score = 130 bits (326), Expect = 2e-28
Identities = 60/107 (56%), Positives = 75/107 (70%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KT QQWGD R +PGY G PRMQIWHGT+D TLRYPN+ E + QW+NV GL+ + D
Sbjct: 202 KTPQQWGDLVRNAYPGYTGPRPRMQIWHGTNDETLRYPNFGEQIDQWTNVHGLSQTPDYT 261
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
++PQSGYT T YG GT TA + S QGV H +P+ +AQ + FFG+
Sbjct: 262 DSPQSGYTRTRYG-GTGGTATVEAISMQGVTHNLPVDAAQAIRFFGL 307
>ref|XP_007279078.1| phb depolymerase family esterase [Colletotrichum gloeosporioides
Nara gc5] gi|429856961|gb|ELA31849.1| phb depolymerase
family esterase [Colletotrichum gloeosporioides Nara
gc5]
Length = 290
Score = 129 bits (325), Expect = 3e-28
Identities = 57/107 (53%), Positives = 77/107 (71%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KT +W Q R +PGYNG++PRMQ +HGT+D+ + YPN E +K+WS + G++ +K+
Sbjct: 188 KTGAEWAAQIRAMYPGYNGSYPRMQTFHGTADTLVTYPNLAEQLKEWSTIHGVSFTKNNT 247
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTP SGYT +YGDGT +LVG SAQGVGHTVP+ QDL +FG+
Sbjct: 248 NTPLSGYTQIVYGDGT----KLVGISAQGVGHTVPVQEDQDLKWFGL 290
>ref|XP_007346063.1| PHB depolymerase family esterase [Auricularia delicata TFB-10046
SS5] gi|393238098|gb|EJD45636.1| PHB depolymerase family
esterase [Auricularia delicata TFB-10046 SS5]
Length = 289
Score = 129 bits (324), Expect = 4e-28
Identities = 54/107 (50%), Positives = 76/107 (71%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
K+ +W Q R +PGY+G +P+M IWHGT+D+ + YPN E +KQWS + G+ +++
Sbjct: 187 KSGAEWAAQARAMYPGYSGAYPKMLIWHGTADTFVNYPNMAEELKQWSTIHGVALARNVT 246
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTPQSGYT +YGDG +++VGYSA GVGHTVP+H DL +FG+
Sbjct: 247 NTPQSGYTQMVYGDG----SKVVGYSAAGVGHTVPVHEDVDLQWFGL 289
>gb|ENH80390.1| feruloyl esterase b precursor [Colletotrichum orbiculare MAFF
240422]
Length = 291
Score = 129 bits (323), Expect = 5e-28
Identities = 58/107 (54%), Positives = 77/107 (71%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KT +QW Q R +P YNGT+PR+Q +HGT+D+ + YPN E +K+WS V G+ +K++
Sbjct: 189 KTGEQWAAQVRAMYPAYNGTYPRIQTFHGTADTLVTYPNLGEQLKEWSAVHGVGFTKNST 248
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
NTP SGYT +YGDGT +LVGYSAQGVGHTVP+ DL +FG+
Sbjct: 249 NTPLSGYTQIVYGDGT----KLVGYSAQGVGHTVPVQEDLDLKWFGL 291
>ref|WP_007461339.1| esterase [Micromonospora lupini] gi|385884820|emb|CCH19305.1|
Esterase (Cellulose-binding domain) [Micromonospora
lupini str. Lupac 08]
Length = 448
Score = 128 bits (321), Expect = 9e-28
Identities = 60/107 (56%), Positives = 75/107 (70%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
KTAQQWGD RG +PGY G PRMQIWHGTSD TLRYPN+ E +KQW+NV GL+ +
Sbjct: 198 KTAQQWGDLVRGAYPGYTGRRPRMQIWHGTSDETLRYPNFGEQIKQWTNVHGLSQTPTYT 257
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
++PQSGYT T YG + A + S QGV H +P+ +AQ + FFG+
Sbjct: 258 DSPQSGYTRTRYG-SSGPMAPVEAISMQGVTHNLPVDAAQVIRFFGL 303
>ref|WP_020523700.1| hypothetical protein [Catelliglobosispora koreensis]
Length = 430
Score = 127 bits (319), Expect = 2e-27
Identities = 59/107 (55%), Positives = 73/107 (68%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTA 162
+T QQWGD R +PGY G PRMQIWHGT+D LRYPN+ E +KQW+NV GL+ +
Sbjct: 198 RTPQQWGDLVRNAYPGYTGARPRMQIWHGTNDEILRYPNFGEQVKQWTNVHGLSQTPSFT 257
Query: 161 NTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
+TPQSGYT T YG GT A + S QGV H +P+ +AQ L FFG+
Sbjct: 258 DTPQSGYTRTRYG-GTGGMAPVEAISMQGVSHNLPVDAAQVLRFFGL 303
>emb|CCA74360.1| probable acetylxylan esterase [Piriformospora indica DSM 11827]
Length = 364
Score = 127 bits (318), Expect = 2e-27
Identities = 60/109 (55%), Positives = 74/109 (67%), Gaps = 2/109 (1%)
Frame = -1
Query: 341 KTAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGL--TASKD 168
KTA WGD R +PGY G P+M IW GT+D+TL Y NY E +KQW+NVLG+ T +
Sbjct: 185 KTASAWGDLVRAAYPGYTGPRPKMMIWDGTADTTLNYANYAEMLKQWTNVLGVSQTPTTT 244
Query: 167 TANTPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGI 21
+ N PQSGYT T YGD ++GYS QGVGHTVP+H +LA+FGI
Sbjct: 245 STNNPQSGYTKTTYGD------VVIGYSVQGVGHTVPVHEDIELAWFGI 287
>gb|EHK46536.1| carbohydrate esterase family 1 protein [Trichoderma atroviride IMI
206040]
Length = 348
Score = 126 bits (317), Expect = 3e-27
Identities = 58/107 (54%), Positives = 73/107 (68%)
Frame = -1
Query: 338 TAQQWGDQTRGYFPGYNGTWPRMQIWHGTSDSTLRYPNYQETMKQWSNVLGLTASKDTAN 159
TAQQWGD R +PGY G +P+MQ+W GT+D+ + N Q + QWSNV+GL+ S + N
Sbjct: 184 TAQQWGDIARAEYPGYTGQYPKMQVWEGTADTLVTIGNLQAQLDQWSNVMGLSFSHNETN 243
Query: 158 TPQSGYTMTIYGDGTAATAQLVGYSAQGVGHTVPIHSAQDLAFFGIV 18
TPQSGYT +YGDGT +LVGYSA GVGH VP H L FF ++
Sbjct: 244 TPQSGYTKIVYGDGT----RLVGYSAAGVGHFVPFHETDVLDFFDLL 286