BLASTX nr result
ID: Akebia23_contig00053461
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Akebia23_contig00053461
(285 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EPE25650.1| ALDH-like protein [Glarea lozoyensis ATCC 20868] 180 2e-43
gb|EHK99035.1| putative Aldehyde dehydrogenase [Glarea lozoyensi... 180 2e-43
ref|XP_003849597.1| aldehyde dehydrogenase [Zymoseptoria tritici... 180 2e-43
gb|EUC45567.1| hypothetical protein COCMIDRAFT_95216 [Bipolaris ... 179 4e-43
gb|EUC30383.1| hypothetical protein COCCADRAFT_7575 [Bipolaris z... 179 5e-43
gb|EMD91526.1| hypothetical protein COCHEDRAFT_1224654 [Bipolari... 179 5e-43
ref|XP_007293107.1| putative Aldehyde dehydrogenase [Marssonina ... 179 5e-43
gb|EMD61608.1| hypothetical protein COCSADRAFT_28936 [Bipolaris ... 178 8e-43
ref|XP_001554714.1| hypothetical protein BC1G_06362 [Botryotinia... 177 1e-42
gb|EOA82024.1| hypothetical protein SETTUDRAFT_165532 [Setosphae... 176 2e-42
gb|EON61515.1| aldehyde dehydrogenase [Coniosporium apollinis CB... 175 7e-42
ref|XP_001797286.1| hypothetical protein SNOG_06925 [Phaeosphaer... 174 1e-41
sp|P40108.2|ALDH_CLAHE RecName: Full=Aldehyde dehydrogenase; Sho... 173 2e-41
gb|ELR02041.1| aldehyde dehydrogenase [Pseudogymnoascus destruct... 173 3e-41
gb|EMF09654.1| aldehyde dehydrogenase [Sphaerulina musiva SO2202] 172 3e-41
ref|XP_003835866.1| similar to aldehyde dehydrogenase [Leptospha... 172 3e-41
gb|AAF82789.1|AF275347_1 aldehyde dehydrogenase [Passalora fulva] 172 4e-41
gb|EME41123.1| hypothetical protein DOTSEDRAFT_27698 [Dothistrom... 172 6e-41
gb|EMC91923.1| hypothetical protein BAUCODRAFT_311185 [Baudoinia... 172 6e-41
sp|P42041.2|ALDH_ALTAL RecName: Full=Aldehyde dehydrogenase; Sho... 172 6e-41
>gb|EPE25650.1| ALDH-like protein [Glarea lozoyensis ATCC 20868]
Length = 496
Score = 180 bits (456), Expect = 2e-43
Identities = 87/94 (92%), Positives = 92/94 (97%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMWSWKIGPA+A GNTVV+KTAEQTPLSA VAA+LVKEAGFPPGVIN+ISGF
Sbjct: 160 IIPWNFPLLMWSWKIGPAVATGNTVVLKTAEQTPLSALVAANLVKEAGFPPGVINIISGF 219
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAAIS+HMDVDKVAFTGSTVVGRQIMKAAA
Sbjct: 220 GKVAGAAISSHMDVDKVAFTGSTVVGRQIMKAAA 253
>gb|EHK99035.1| putative Aldehyde dehydrogenase [Glarea lozoyensis 74030]
Length = 469
Score = 180 bits (456), Expect = 2e-43
Identities = 87/94 (92%), Positives = 92/94 (97%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMWSWKIGPA+A GNTVV+KTAEQTPLSA VAA+LVKEAGFPPGVIN+ISGF
Sbjct: 160 IIPWNFPLLMWSWKIGPAVATGNTVVLKTAEQTPLSALVAANLVKEAGFPPGVINIISGF 219
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAAIS+HMDVDKVAFTGSTVVGRQIMKAAA
Sbjct: 220 GKVAGAAISSHMDVDKVAFTGSTVVGRQIMKAAA 253
>ref|XP_003849597.1| aldehyde dehydrogenase [Zymoseptoria tritici IPO323]
gi|339469474|gb|EGP84573.1| aldehyde dehydrogenase
[Zymoseptoria tritici IPO323]
Length = 497
Score = 180 bits (456), Expect = 2e-43
Identities = 88/94 (93%), Positives = 92/94 (97%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMWSWKIGPAIAAGNTVV+KTAEQTPLSA VAA+L+KEAGFPPGVINVISGF
Sbjct: 161 IIPWNFPLLMWSWKIGPAIAAGNTVVLKTAEQTPLSALVAANLIKEAGFPPGVINVISGF 220
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAAISTHMDVDKVAFTGSTVVGR I+KAAA
Sbjct: 221 GKVAGAAISTHMDVDKVAFTGSTVVGRTILKAAA 254
>gb|EUC45567.1| hypothetical protein COCMIDRAFT_95216 [Bipolaris oryzae ATCC 44560]
Length = 499
Score = 179 bits (454), Expect = 4e-43
Identities = 86/94 (91%), Positives = 92/94 (97%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFP+LMWSWKIGPAIAAGNTVV+KTAEQTPLSA+VA L+KEAGFPPGVINVI+GF
Sbjct: 163 IIPWNFPILMWSWKIGPAIAAGNTVVLKTAEQTPLSAYVACKLIKEAGFPPGVINVITGF 222
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAAIS+HMDVDKVAFTGSTVVGRQIMKAAA
Sbjct: 223 GKVAGAAISSHMDVDKVAFTGSTVVGRQIMKAAA 256
>gb|EUC30383.1| hypothetical protein COCCADRAFT_7575 [Bipolaris zeicola 26-R-13]
gi|578486046|gb|EUN23529.1| hypothetical protein
COCVIDRAFT_18950 [Bipolaris victoriae FI3]
Length = 499
Score = 179 bits (453), Expect = 5e-43
Identities = 86/94 (91%), Positives = 91/94 (96%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFP+LMWSWKIGPAIAAGNTVV+KTAEQTPLSA+VA L+KEAGFPPGVINVI+GF
Sbjct: 163 IIPWNFPILMWSWKIGPAIAAGNTVVLKTAEQTPLSAYVACKLIKEAGFPPGVINVITGF 222
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAAIS HMDVDKVAFTGSTVVGRQIMKAAA
Sbjct: 223 GKVAGAAISAHMDVDKVAFTGSTVVGRQIMKAAA 256
>gb|EMD91526.1| hypothetical protein COCHEDRAFT_1224654 [Bipolaris maydis C5]
gi|477591644|gb|ENI08716.1| hypothetical protein
COCC4DRAFT_128613 [Bipolaris maydis ATCC 48331]
Length = 499
Score = 179 bits (453), Expect = 5e-43
Identities = 86/94 (91%), Positives = 91/94 (96%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFP+LMWSWKIGPAIAAGNTVV+KTAEQTPLSA+VA L+KEAGFPPGVINVI+GF
Sbjct: 163 IIPWNFPILMWSWKIGPAIAAGNTVVLKTAEQTPLSAYVACKLIKEAGFPPGVINVITGF 222
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAAIS HMDVDKVAFTGSTVVGRQIMKAAA
Sbjct: 223 GKVAGAAISAHMDVDKVAFTGSTVVGRQIMKAAA 256
>ref|XP_007293107.1| putative Aldehyde dehydrogenase [Marssonina brunnea f. sp.
'multigermtubi' MB_m1] gi|406863702|gb|EKD16749.1|
putative Aldehyde dehydrogenase [Marssonina brunnea f.
sp. 'multigermtubi' MB_m1]
Length = 638
Score = 179 bits (453), Expect = 5e-43
Identities = 85/94 (90%), Positives = 91/94 (96%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMWSWKIGPA+AAGNTVV+KTAEQTPLSA VAAS +KEAGFPPGVIN+ISGF
Sbjct: 302 IIPWNFPLLMWSWKIGPAVAAGNTVVLKTAEQTPLSALVAASFIKEAGFPPGVINIISGF 361
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
G+VAGAAIS HMDVDKVAFTGSTVVGRQ+MKAAA
Sbjct: 362 GRVAGAAISAHMDVDKVAFTGSTVVGRQVMKAAA 395
>gb|EMD61608.1| hypothetical protein COCSADRAFT_28936 [Bipolaris sorokiniana
ND90Pr]
Length = 499
Score = 178 bits (451), Expect = 8e-43
Identities = 84/94 (89%), Positives = 91/94 (96%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFP+LMWSWKIGPA+AAGNTVV+KTAEQTPLSA+VA L+KEAGFPPGVINVI+GF
Sbjct: 163 IIPWNFPILMWSWKIGPAVAAGNTVVLKTAEQTPLSAYVACKLIKEAGFPPGVINVITGF 222
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAAIS HMD+DKVAFTGSTVVGRQIMKAAA
Sbjct: 223 GKVAGAAISAHMDIDKVAFTGSTVVGRQIMKAAA 256
>ref|XP_001554714.1| hypothetical protein BC1G_06362 [Botryotinia fuckeliana B05.10]
gi|347440923|emb|CCD33844.1| similar to aldehyde
dehydrogenase [Botryotinia fuckeliana T4]
gi|472235574|gb|EMR80546.1| putative aldehyde
dehydrogenase protein [Botryotinia fuckeliana BcDW1]
Length = 496
Score = 177 bits (450), Expect = 1e-42
Identities = 83/94 (88%), Positives = 92/94 (97%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMWSWKIGPA+A GNTVV+KTAEQTPLSA VAA+L+KEAGFPPGV+N+ISGF
Sbjct: 160 IIPWNFPLLMWSWKIGPAVATGNTVVLKTAEQTPLSALVAANLIKEAGFPPGVVNIISGF 219
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GK+AGAAI++HMDVDKVAFTGSTVVGRQIMKAAA
Sbjct: 220 GKIAGAAIASHMDVDKVAFTGSTVVGRQIMKAAA 253
>gb|EOA82024.1| hypothetical protein SETTUDRAFT_165532 [Setosphaeria turcica Et28A]
Length = 499
Score = 176 bits (447), Expect = 2e-42
Identities = 84/94 (89%), Positives = 91/94 (96%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMW WKIGPAIAAGNTVV+KTAEQTPLSA+VA L+KEAGFPPGVINVI+GF
Sbjct: 163 IIPWNFPLLMWCWKIGPAIAAGNTVVLKTAEQTPLSAYVACKLIKEAGFPPGVINVITGF 222
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GK+AGAAI++HMDVDKVAFTGSTVVGRQIMKAAA
Sbjct: 223 GKIAGAAIASHMDVDKVAFTGSTVVGRQIMKAAA 256
>gb|EON61515.1| aldehyde dehydrogenase [Coniosporium apollinis CBS 100218]
Length = 496
Score = 175 bits (443), Expect = 7e-42
Identities = 83/94 (88%), Positives = 90/94 (95%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMW+WKIGPA+A GNTVVIKTAEQTPLSA +AA LVKEAGFPPGVINV+SGF
Sbjct: 160 IIPWNFPLLMWAWKIGPAVACGNTVVIKTAEQTPLSALIAAGLVKEAGFPPGVINVLSGF 219
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GK+AGAAI++HMDVDKVAFTGSTVVGRQIMK AA
Sbjct: 220 GKIAGAAIASHMDVDKVAFTGSTVVGRQIMKMAA 253
>ref|XP_001797286.1| hypothetical protein SNOG_06925 [Phaeosphaeria nodorum SN15]
gi|111064456|gb|EAT85576.1| hypothetical protein
SNOG_06925 [Phaeosphaeria nodorum SN15]
Length = 499
Score = 174 bits (441), Expect = 1e-41
Identities = 81/94 (86%), Positives = 91/94 (96%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMW+WKIGPAIA GN VVIKTAEQTPLSA++AA+L+KEAGFPPGVINVI+GF
Sbjct: 163 IIPWNFPLLMWAWKIGPAIATGNCVVIKTAEQTPLSAYIAANLIKEAGFPPGVINVITGF 222
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GK+AGAA+S HMDVDK+AFTGSTVVGRQIMK+AA
Sbjct: 223 GKIAGAALSAHMDVDKIAFTGSTVVGRQIMKSAA 256
>sp|P40108.2|ALDH_CLAHE RecName: Full=Aldehyde dehydrogenase; Short=ALDDH; Short=ALDH;
AltName: Full=Allergen Cla h 3; AltName: Full=Allergen
Cla h III; AltName: Allergen=Cla h 10
gi|76666769|emb|CAA55072.2| aldehyde dehydrogenase,
allergen Cla h 10 [Cladosporium herbarum]
Length = 496
Score = 173 bits (439), Expect = 2e-41
Identities = 84/94 (89%), Positives = 89/94 (94%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMW+WKIGPAIA GNTVV+KTAEQTPL VAASLVKEAGFPPGVINVISGF
Sbjct: 160 IIPWNFPLLMWAWKIGPAIACGNTVVLKTAEQTPLGGLVAASLVKEAGFPPGVINVISGF 219
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAA+S+HMDVDKVAFTGSTVVGR I+KAAA
Sbjct: 220 GKVAGAALSSHMDVDKVAFTGSTVVGRTILKAAA 253
>gb|ELR02041.1| aldehyde dehydrogenase [Pseudogymnoascus destructans 20631-21]
Length = 497
Score = 173 bits (438), Expect = 3e-41
Identities = 82/94 (87%), Positives = 89/94 (94%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMWSWKIGPAIA GNTVV+KTAEQTPLSA AA L+KEAGFPPGVIN+ISGF
Sbjct: 161 IIPWNFPLLMWSWKIGPAIATGNTVVLKTAEQTPLSALFAAKLIKEAGFPPGVINIISGF 220
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GK AGAA+S+HMDVDKVAFTGST+VGRQI+KAAA
Sbjct: 221 GKTAGAALSSHMDVDKVAFTGSTLVGRQILKAAA 254
>gb|EMF09654.1| aldehyde dehydrogenase [Sphaerulina musiva SO2202]
Length = 498
Score = 172 bits (437), Expect = 3e-41
Identities = 82/94 (87%), Positives = 88/94 (93%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMW+WKIGPAIAAGN VV+KTAEQTPL VAA L+KEAGFPPGV+NVISGF
Sbjct: 162 IIPWNFPLLMWAWKIGPAIAAGNVVVLKTAEQTPLGGLVAAQLIKEAGFPPGVVNVISGF 221
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAA+S HMDVDKVAFTGSTVVGRQI+KAAA
Sbjct: 222 GKVAGAALSAHMDVDKVAFTGSTVVGRQILKAAA 255
>ref|XP_003835866.1| similar to aldehyde dehydrogenase [Leptosphaeria maculans JN3]
gi|312212418|emb|CBX92501.1| similar to aldehyde
dehydrogenase [Leptosphaeria maculans JN3]
Length = 497
Score = 172 bits (437), Expect = 3e-41
Identities = 81/94 (86%), Positives = 91/94 (96%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMW+WKIGPAIA GNTVV+KTAEQTPLSA VA +L+++AGFPPGVINVISGF
Sbjct: 161 IIPWNFPLLMWAWKIGPAIACGNTVVLKTAEQTPLSALVACTLIQKAGFPPGVINVISGF 220
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAA+S+HMD+DK+AFTGSTVVGRQIMKAAA
Sbjct: 221 GKVAGAAMSSHMDIDKIAFTGSTVVGRQIMKAAA 254
>gb|AAF82789.1|AF275347_1 aldehyde dehydrogenase [Passalora fulva]
Length = 497
Score = 172 bits (436), Expect = 4e-41
Identities = 80/94 (85%), Positives = 90/94 (95%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMW+WKIGPA+A GNTVVIKTAEQTPL A+VAA+L+KEAGFPPGV+NVISGF
Sbjct: 161 IIPWNFPLLMWAWKIGPAVATGNTVVIKTAEQTPLGAYVAANLIKEAGFPPGVVNVISGF 220
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAAI++HMD+DKVAFTGSTV GR I+KAAA
Sbjct: 221 GKVAGAAIASHMDIDKVAFTGSTVTGRTILKAAA 254
>gb|EME41123.1| hypothetical protein DOTSEDRAFT_27698 [Dothistroma septosporum
NZE10]
Length = 498
Score = 172 bits (435), Expect = 6e-41
Identities = 80/94 (85%), Positives = 90/94 (95%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMW+WKIGPA+A GNTVVIKTAEQTPL A+VAA+L+KEAGFPPGV+NVISGF
Sbjct: 162 IIPWNFPLLMWAWKIGPAVATGNTVVIKTAEQTPLGAYVAANLIKEAGFPPGVVNVISGF 221
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAA+++HMDVDKVAFTGSTV GR I+KAAA
Sbjct: 222 GKVAGAALASHMDVDKVAFTGSTVTGRTILKAAA 255
>gb|EMC91923.1| hypothetical protein BAUCODRAFT_311185 [Baudoinia compniacensis
UAMH 10762]
Length = 498
Score = 172 bits (435), Expect = 6e-41
Identities = 83/94 (88%), Positives = 88/94 (93%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFPLLMW+WKIGPA+A GNTVV+KTAEQTPL VAA LVKEAGFPPGVINVISGF
Sbjct: 162 IIPWNFPLLMWAWKIGPAVACGNTVVLKTAEQTPLGGLVAARLVKEAGFPPGVINVISGF 221
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GKVAGAAI++HMDVDKVAFTGSTVVGR IMKAAA
Sbjct: 222 GKVAGAAIASHMDVDKVAFTGSTVVGRTIMKAAA 255
>sp|P42041.2|ALDH_ALTAL RecName: Full=Aldehyde dehydrogenase; Short=ALDDH; Short=ALDH;
AltName: Full=Allergen Alt a X; AltName: Allergen=Alt a
10 gi|76666767|emb|CAA55071.2| aldehyde dehydrogenase
(NAD+) [Alternaria alternata]
Length = 497
Score = 172 bits (435), Expect = 6e-41
Identities = 78/94 (82%), Positives = 90/94 (95%)
Frame = -3
Query: 283 IIPWNFPLLMWSWKIGPAIAAGNTVVIKTAEQTPLSAFVAASLVKEAGFPPGVINVISGF 104
IIPWNFP+LMWSWKIGPAIA GNTVV+KTAEQTPLSA++A L++EAGFPPGVINVI+GF
Sbjct: 161 IIPWNFPILMWSWKIGPAIATGNTVVLKTAEQTPLSAYIACKLIQEAGFPPGVINVITGF 220
Query: 103 GKVAGAAISTHMDVDKVAFTGSTVVGRQIMKAAA 2
GK+AGAA+S HMD+DK+AFTGSTVVGRQIMK+AA
Sbjct: 221 GKIAGAAMSAHMDIDKIAFTGSTVVGRQIMKSAA 254