BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia22_contig00034136
         (303 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004237761.1| PREDICTED: pentatricopeptide repeat-containi...   104   1e-20
ref|XP_006356287.1| PREDICTED: pentatricopeptide repeat-containi...   102   6e-20
ref|XP_002278218.1| PREDICTED: pentatricopeptide repeat-containi...    99   8e-19
ref|XP_002307479.2| pentatricopeptide repeat-containing family p...    90   3e-16
ref|XP_007019396.1| Pentatricopeptide repeat superfamily protein...    90   4e-16
ref|XP_006407716.1| hypothetical protein EUTSA_v10019974mg [Eutr...    89   6e-16
ref|XP_006434387.1| hypothetical protein CICLE_v10003408mg, part...    85   9e-15
ref|XP_006472926.1| PREDICTED: pentatricopeptide repeat-containi...    84   3e-14
ref|NP_187516.1| pentatricopeptide repeat-containing protein [Ar...    82   1e-13
gb|ADN34182.1| pentatricopeptide repeat-containing protein [Cucu...    81   1e-13
ref|XP_007199369.1| hypothetical protein PRUPE_ppa025439mg [Prun...    80   3e-13
ref|XP_004154496.1| PREDICTED: LOW QUALITY PROTEIN: pentatricope...    80   4e-13
ref|XP_004139740.1| PREDICTED: LOW QUALITY PROTEIN: pentatricope...    80   4e-13
ref|XP_006299083.1| hypothetical protein CARUB_v10015238mg [Caps...    79   5e-13
ref|XP_004292543.1| PREDICTED: pentatricopeptide repeat-containi...    79   7e-13
ref|XP_002884708.1| pentatricopeptide repeat-containing protein ...    79   7e-13
gb|EXB79430.1| hypothetical protein L484_011623 [Morus notabilis]      60   3e-07
ref|XP_007203614.1| hypothetical protein PRUPE_ppa002292mg [Prun...    58   2e-06
gb|EPS65716.1| hypothetical protein M569_09060, partial [Genlise...    57   2e-06
ref|XP_004154387.1| PREDICTED: pentatricopeptide repeat-containi...    56   5e-06

>ref|XP_004237761.1| PREDICTED: pentatricopeptide repeat-containing protein At3g09040,
           mitochondrial-like [Solanum lycopersicum]
          Length = 1021

 Score =  104 bits (259), Expect = 1e-20
 Identities = 52/100 (52%), Positives = 65/100 (65%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           +Y  LLK CLQEC+ +QSRR+ DEMPQR  ++VK CK +H Q+               VD
Sbjct: 45  LYDNLLKICLQECKNLQSRRVFDEMPQRVARAVKACKTIHLQSLKLGFASQGHLGNSIVD 104

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLPE 300
           LYAKCGD+  A KAF  L+ +DG AWNSI+ +YSR GL E
Sbjct: 105 LYAKCGDMVSAEKAFFWLENKDGMAWNSIILMYSRNGLLE 144


>ref|XP_006356287.1| PREDICTED: pentatricopeptide repeat-containing protein At3g09040,
           mitochondrial-like isoform X1 [Solanum tuberosum]
           gi|565379764|ref|XP_006356288.1| PREDICTED:
           pentatricopeptide repeat-containing protein At3g09040,
           mitochondrial-like isoform X2 [Solanum tuberosum]
           gi|565379766|ref|XP_006356289.1| PREDICTED:
           pentatricopeptide repeat-containing protein At3g09040,
           mitochondrial-like isoform X3 [Solanum tuberosum]
           gi|565379768|ref|XP_006356290.1| PREDICTED:
           pentatricopeptide repeat-containing protein At3g09040,
           mitochondrial-like isoform X4 [Solanum tuberosum]
           gi|565379770|ref|XP_006356291.1| PREDICTED:
           pentatricopeptide repeat-containing protein At3g09040,
           mitochondrial-like isoform X5 [Solanum tuberosum]
          Length = 1028

 Score =  102 bits (254), Expect = 6e-20
 Identities = 51/100 (51%), Positives = 64/100 (64%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           +Y  LLK CLQEC+ +QSRR+ DEMPQR  ++VK CK +H Q+               VD
Sbjct: 45  LYNNLLKICLQECKNLQSRRVFDEMPQRAARAVKACKTIHLQSLKLGFASQGHLGNSIVD 104

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLPE 300
           LYAKCGD+  A KAF  L+ +D  AWNSI+ +YSR GL E
Sbjct: 105 LYAKCGDMVSAEKAFFWLENKDSIAWNSIILMYSRNGLLE 144


>ref|XP_002278218.1| PREDICTED: pentatricopeptide repeat-containing protein At3g09040,
           mitochondrial [Vitis vinifera]
           gi|302142763|emb|CBI19966.3| unnamed protein product
           [Vitis vinifera]
          Length = 1048

 Score = 98.6 bits (244), Expect = 8e-19
 Identities = 50/101 (49%), Positives = 64/101 (63%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           I+T LLK CLQ+C+ ++ R   DE PQR  Q+ +  K +HAQT               VD
Sbjct: 65  IFTHLLKICLQQCQRIKIRHPFDETPQRLAQASRTSKTIHAQTLKFGFGSKGRLGSAIVD 124

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLPEE 303
           LYAKCG+VEFA KAF  L++RD  AWNS+LS+YSR G  E+
Sbjct: 125 LYAKCGNVEFAAKAFNQLEKRDILAWNSVLSMYSRQGSLEQ 165


>ref|XP_002307479.2| pentatricopeptide repeat-containing family protein [Populus
           trichocarpa] gi|550339428|gb|EEE94475.2|
           pentatricopeptide repeat-containing family protein
           [Populus trichocarpa]
          Length = 1026

 Score = 90.1 bits (222), Expect = 3e-16
 Identities = 44/99 (44%), Positives = 64/99 (64%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           IYT +L+ CLQ+ + V++  L DE+PQR +Q     KI+HAQ+               VD
Sbjct: 43  IYTHILQNCLQKSKQVKTHSLFDEIPQRLSQFSTTNKIIHAQSLKLGFWSKGVLGNVIVD 102

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLP 297
           LYAKC DV++A +AF+ L+++D  AWNSILS++S+ G P
Sbjct: 103 LYAKCADVDYAERAFKQLEDKDILAWNSILSMHSKQGFP 141


>ref|XP_007019396.1| Pentatricopeptide repeat superfamily protein [Theobroma cacao]
           gi|508724724|gb|EOY16621.1| Pentatricopeptide repeat
           superfamily protein [Theobroma cacao]
          Length = 1030

 Score = 89.7 bits (221), Expect = 4e-16
 Identities = 45/101 (44%), Positives = 63/101 (62%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           IYT LL+ CLQ+CR V++    DEM  ++ Q++K  K++HA+                +D
Sbjct: 48  IYTQLLRLCLQQCREVKTHYTFDEMSPKREQALKAGKMIHARGLKLGFWSKGLLGNAILD 107

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLPEE 303
           LYAKCGDV+ A KAF  L++RD  AWNS++ +YS  GL EE
Sbjct: 108 LYAKCGDVDSAEKAFHGLEKRDVLAWNSVILMYSNRGLVEE 148


>ref|XP_006407716.1| hypothetical protein EUTSA_v10019974mg [Eutrema salsugineum]
           gi|557108862|gb|ESQ49169.1| hypothetical protein
           EUTSA_v10019974mg [Eutrema salsugineum]
          Length = 1023

 Score = 89.0 bits (219), Expect = 6e-16
 Identities = 43/99 (43%), Positives = 61/99 (61%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           IY  LL+TCL++C+L++SR++ D MPQR   +++  K +H+Q+               VD
Sbjct: 39  IYDTLLETCLEQCKLIKSRKVFDGMPQRLVHALRTGKAVHSQSLVLGIDLKGRLGNAIVD 98

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLP 297
           LYAKC  V +A K F  L E+D   WNS+LS+YS  GLP
Sbjct: 99  LYAKCAQVSYAEKVFNSL-EKDVMTWNSMLSMYSSIGLP 136


>ref|XP_006434387.1| hypothetical protein CICLE_v10003408mg, partial [Citrus clementina]
           gi|557536509|gb|ESR47627.1| hypothetical protein
           CICLE_v10003408mg, partial [Citrus clementina]
          Length = 1003

 Score = 85.1 bits (209), Expect = 9e-15
 Identities = 42/97 (43%), Positives = 60/97 (61%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           IYT LL++CLQ+C+ +++R + D   QR  ++    +I+HAQ+               VD
Sbjct: 25  IYTHLLESCLQQCKQIKTRHMFDGSSQRLIRASITSRIIHAQSLKFGFGSKGLLGNAIVD 84

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCG 291
           LYAKCG V  A K F+ L++RD  AWNSILS+YS+ G
Sbjct: 85  LYAKCGIVNLAEKVFDRLEDRDILAWNSILSMYSKRG 121


>ref|XP_006472926.1| PREDICTED: pentatricopeptide repeat-containing protein At3g09040,
           mitochondrial-like [Citrus sinensis]
          Length = 1017

 Score = 83.6 bits (205), Expect = 3e-14
 Identities = 41/97 (42%), Positives = 59/97 (60%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           IYT LL++CLQ+C+ +++R + D   QR  ++    +I+HAQ+               VD
Sbjct: 39  IYTHLLESCLQQCKQIKTRHMFDGSSQRLIRASITSRIIHAQSLKFGFGSKGLLGNAIVD 98

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCG 291
           LYAKCG    A K F+ L++RD  AWNSILS+YS+ G
Sbjct: 99  LYAKCGIANLAEKVFDRLEDRDILAWNSILSMYSKRG 135


>ref|NP_187516.1| pentatricopeptide repeat-containing protein [Arabidopsis thaliana]
           gi|75207468|sp|Q9SS83.1|PP220_ARATH RecName:
           Full=Pentatricopeptide repeat-containing protein
           At3g09040, mitochondrial; Flags: Precursor
           gi|5923669|gb|AAD56320.1|AC009326_7 hypothetical protein
           [Arabidopsis thaliana] gi|332641192|gb|AEE74713.1|
           pentatricopeptide repeat-containing protein [Arabidopsis
           thaliana]
          Length = 1028

 Score = 81.6 bits (200), Expect = 1e-13
 Identities = 41/99 (41%), Positives = 61/99 (61%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           I+  LL+ CL +C+L +SR++ DEMPQR   +++  K +H+++               VD
Sbjct: 44  IHQRLLEICLGQCKLFKSRKVFDEMPQRLALALRIGKAVHSKSLILGIDSEGRLGNAIVD 103

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLP 297
           LYAKC  V +A K F+ L E+D +AWNS+LS+YS  G P
Sbjct: 104 LYAKCAQVSYAEKQFDFL-EKDVTAWNSMLSMYSSIGKP 141


>gb|ADN34182.1| pentatricopeptide repeat-containing protein [Cucumis melo subsp.
           melo]
          Length = 1131

 Score = 81.3 bits (199), Expect = 1e-13
 Identities = 37/93 (39%), Positives = 57/93 (61%)
 Frame = +1

Query: 16  LKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVDLYAKC 195
           L+ CLQ CR +Q+  L +E P+   Q++   K++H+++               VDLY KC
Sbjct: 30  LQICLQHCRRIQAHNLFNEKPKAVLQALSTAKVIHSKSLKIGVGLKGLLGNVIVDLYVKC 89

Query: 196 GDVEFARKAFELLDERDGSAWNSILSLYSRCGL 294
           G+V+FA+KAF  L+++D  AWNS+LS+Y   GL
Sbjct: 90  GNVDFAQKAFSRLEKKDVFAWNSVLSMYLDHGL 122


>ref|XP_007199369.1| hypothetical protein PRUPE_ppa025439mg [Prunus persica]
           gi|462394769|gb|EMJ00568.1| hypothetical protein
           PRUPE_ppa025439mg [Prunus persica]
          Length = 1015

 Score = 80.1 bits (196), Expect = 3e-13
 Identities = 37/93 (39%), Positives = 58/93 (62%), Gaps = 1/93 (1%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRK-TQSVKNCKILHAQTXXXXXXXXXXXXXXXV 177
           +YT LL+ C+Q+C+ +++ ++ DEMP+R   Q+ + C+ +HAQ+               V
Sbjct: 36  LYTHLLQICIQQCKNIKTHKVFDEMPERLLAQASRTCETVHAQSLKFGVGSKGFLGNAIV 95

Query: 178 DLYAKCGDVEFARKAFELLDERDGSAWNSILSL 276
             YAKCG+V FA KAF  L+ +D  AWNS+LS+
Sbjct: 96  GFYAKCGNVGFAEKAFNCLENKDVFAWNSVLSM 128


>ref|XP_004154496.1| PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing
           protein At3g09040, mitochondrial-like [Cucumis sativus]
          Length = 974

 Score = 79.7 bits (195), Expect = 4e-13
 Identities = 37/93 (39%), Positives = 56/93 (60%)
 Frame = +1

Query: 16  LKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVDLYAKC 195
           L+ CLQ C  +Q+  L DE P+   Q++   K++H+++               VDLY KC
Sbjct: 30  LQICLQHCWRIQAHNLFDEKPKPVLQALSTAKVIHSKSLKIGVGLKGLLGNVIVDLYVKC 89

Query: 196 GDVEFARKAFELLDERDGSAWNSILSLYSRCGL 294
           G+V+FA+KAF  L+++D  AWNS+LS+Y   GL
Sbjct: 90  GNVDFAQKAFSRLEKKDVFAWNSVLSMYLDHGL 122


>ref|XP_004139740.1| PREDICTED: LOW QUALITY PROTEIN: pentatricopeptide repeat-containing
           protein At3g09040, mitochondrial-like [Cucumis sativus]
          Length = 1441

 Score = 79.7 bits (195), Expect = 4e-13
 Identities = 37/93 (39%), Positives = 56/93 (60%)
 Frame = +1

Query: 16  LKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVDLYAKC 195
           L+ CLQ C  +Q+  L DE P+   Q++   K++H+++               VDLY KC
Sbjct: 30  LQICLQHCWRIQAHNLFDEKPKPVLQALSTAKVIHSKSLKIGVGLKGLLGNVIVDLYVKC 89

Query: 196 GDVEFARKAFELLDERDGSAWNSILSLYSRCGL 294
           G+V+FA+KAF  L+++D  AWNS+LS+Y   GL
Sbjct: 90  GNVDFAQKAFSRLEKKDVFAWNSVLSMYLDHGL 122


>ref|XP_006299083.1| hypothetical protein CARUB_v10015238mg [Capsella rubella]
           gi|482567792|gb|EOA31981.1| hypothetical protein
           CARUB_v10015238mg [Capsella rubella]
          Length = 1028

 Score = 79.3 bits (194), Expect = 5e-13
 Identities = 40/101 (39%), Positives = 62/101 (61%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           I+  LL+ CL++C+L +SR++ DEMPQR   +++  K +H+++               VD
Sbjct: 44  IHQRLLEICLEQCKLFKSRKVFDEMPQRLALALRTGKAVHSKSLILGFGSQGSLGNAIVD 103

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLPEE 303
           LYAKC  V +A K F+ L E+D +A NS+LS+YS  G P +
Sbjct: 104 LYAKCAHVSYAEKLFDYL-EKDVTACNSMLSMYSSIGQPRQ 143


>ref|XP_004292543.1| PREDICTED: pentatricopeptide repeat-containing protein At3g09040,
           mitochondrial-like [Fragaria vesca subsp. vesca]
          Length = 1018

 Score = 79.0 bits (193), Expect = 7e-13
 Identities = 37/102 (36%), Positives = 61/102 (59%), Gaps = 1/102 (0%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRK-TQSVKNCKILHAQTXXXXXXXXXXXXXXXV 177
           ++T L + C ++CRL+++ ++  EM +R+  Q+ + CK +HAQ                V
Sbjct: 33  LHTHLFQICREQCRLIKTHKVFGEMSERELAQASRTCKAIHAQGLKFEFGSKGLLGNGIV 92

Query: 178 DLYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLPEE 303
             YAKCG++ +A KAF  L+ +D  AWNS+LS+YS  GL ++
Sbjct: 93  GFYAKCGNLGYAEKAFNCLENKDVFAWNSVLSMYSNKGLLDQ 134


>ref|XP_002884708.1| pentatricopeptide repeat-containing protein [Arabidopsis lyrata
           subsp. lyrata] gi|297330548|gb|EFH60967.1|
           pentatricopeptide repeat-containing protein [Arabidopsis
           lyrata subsp. lyrata]
          Length = 1028

 Score = 79.0 bits (193), Expect = 7e-13
 Identities = 40/99 (40%), Positives = 60/99 (60%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           I+  LL+ CL++C+L +SR++ DEMP R   +++  K +H+++               VD
Sbjct: 44  IHQGLLEICLEQCKLFKSRKVFDEMPHRLALALRIGKAVHSKSLILGIDSEGRLGNAIVD 103

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLP 297
           LYAKC  V +A K F  L E+D +AWNS+LS+YS  G P
Sbjct: 104 LYAKCAQVSYAEKQFNSL-EKDVTAWNSMLSMYSSIGQP 141


>gb|EXB79430.1| hypothetical protein L484_011623 [Morus notabilis]
          Length = 1230

 Score = 60.1 bits (144), Expect = 3e-07
 Identities = 31/65 (47%), Positives = 40/65 (61%)
 Frame = +1

Query: 109 KILHAQTXXXXXXXXXXXXXXXVDLYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRC 288
           K++HA +               VDLYAK G+V FA KAF  L+++D  AWNSILS+YSR 
Sbjct: 96  KVIHAHSLKLGVASKGLLGNVIVDLYAKGGNVSFAEKAFTQLEKKDVFAWNSILSMYSRW 155

Query: 289 GLPEE 303
           GL E+
Sbjct: 156 GLLEK 160


>ref|XP_007203614.1| hypothetical protein PRUPE_ppa002292mg [Prunus persica]
           gi|462399145|gb|EMJ04813.1| hypothetical protein
           PRUPE_ppa002292mg [Prunus persica]
          Length = 691

 Score = 57.8 bits (138), Expect = 2e-06
 Identities = 33/98 (33%), Positives = 49/98 (50%)
 Frame = +1

Query: 1   IYTLLLKTCLQECRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVD 180
           IY+ LL+ CLQ+  LVQ                   K++HA T               +D
Sbjct: 87  IYSTLLQLCLQQRALVQG------------------KLVHAHTKVSGFVPGLFICNRLID 128

Query: 181 LYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGL 294
           LYAKCG +  A+K F+ + ERD  +WN+++S Y++ GL
Sbjct: 129 LYAKCGSLVDAQKVFDEMSERDLCSWNTMISGYAKVGL 166


>gb|EPS65716.1| hypothetical protein M569_09060, partial [Genlisea aurea]
          Length = 927

 Score = 57.4 bits (137), Expect = 2e-06
 Identities = 31/83 (37%), Positives = 41/83 (49%)
 Frame = +1

Query: 37  CRLVQSRRLLDEMPQRKTQSVKNCKILHAQTXXXXXXXXXXXXXXXVDLYAKCGDVEFAR 216
           C  VQ+R +LD MPQR   S+   + +H Q                 +LYA+CG V +A 
Sbjct: 1   CMTVQARPVLDVMPQRADSSLWTARAVHGQGLRLGISSHGKLGSSISELYARCGHVNYAE 60

Query: 217 KAFELLDERDGSAWNSILSLYSR 285
           K    L+ RD  AWNS L + SR
Sbjct: 61  KMLFGLETRDEMAWNSFLVMKSR 83


>ref|XP_004154387.1| PREDICTED: pentatricopeptide repeat-containing protein At3g46790,
           chloroplastic-like [Cucumis sativus]
           gi|449522468|ref|XP_004168248.1| PREDICTED:
           pentatricopeptide repeat-containing protein At3g46790,
           chloroplastic-like [Cucumis sativus]
          Length = 574

 Score = 56.2 bits (134), Expect = 5e-06
 Identities = 24/43 (55%), Positives = 33/43 (76%)
 Frame = +1

Query: 175 VDLYAKCGDVEFARKAFELLDERDGSAWNSILSLYSRCGLPEE 303
           +D+YAKCG VE AR+ F+ +DERD   WNS+L+ YS+ G P+E
Sbjct: 144 IDMYAKCGCVESARQVFDKIDERDVVCWNSMLATYSQNGQPDE 186