BLASTX nr result
ID: Aconitum23_contig00045044
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum23_contig00045044
(375 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN65543.1| hypothetical protein VITISV_018575 [Vitis vinifera] 97 4e-18
emb|CAN72177.1| hypothetical protein VITISV_012537 [Vitis vinifera] 97 6e-18
emb|CAN81035.1| hypothetical protein VITISV_011008 [Vitis vinifera] 88 2e-15
emb|CAN75374.1| hypothetical protein VITISV_029152 [Vitis vinifera] 87 4e-15
emb|CAN79789.1| hypothetical protein VITISV_039779 [Vitis vinifera] 85 2e-14
emb|CAN75589.1| hypothetical protein VITISV_042880 [Vitis vinifera] 84 4e-14
emb|CAN75784.1| hypothetical protein VITISV_022492 [Vitis vinifera] 84 4e-14
emb|CAN78294.1| hypothetical protein VITISV_035517 [Vitis vinifera] 82 1e-13
ref|XP_006352585.1| PREDICTED: uncharacterized protein LOC102588... 82 2e-13
emb|CAN80245.1| hypothetical protein VITISV_031023 [Vitis vinifera] 82 2e-13
emb|CAN70922.1| hypothetical protein VITISV_016266 [Vitis vinifera] 82 2e-13
emb|CAN74354.1| hypothetical protein VITISV_012842 [Vitis vinifera] 82 2e-13
emb|CAN69899.1| hypothetical protein VITISV_029782 [Vitis vinifera] 82 2e-13
emb|CAN69820.1| hypothetical protein VITISV_025709 [Vitis vinifera] 81 3e-13
emb|CAN70867.1| hypothetical protein VITISV_027610 [Vitis vinifera] 81 3e-13
emb|CAN64680.1| hypothetical protein VITISV_016601 [Vitis vinifera] 80 5e-13
emb|CAN71520.1| hypothetical protein VITISV_042316 [Vitis vinifera] 80 5e-13
emb|CAN68252.1| hypothetical protein VITISV_043921 [Vitis vinifera] 80 8e-13
ref|XP_009599592.1| PREDICTED: uncharacterized protein LOC104095... 79 1e-12
emb|CAN68112.1| hypothetical protein VITISV_040983 [Vitis vinifera] 79 1e-12
>emb|CAN65543.1| hypothetical protein VITISV_018575 [Vitis vinifera]
Length = 185
Score = 97.4 bits (241), Expect = 4e-18
Identities = 45/121 (37%), Positives = 66/121 (54%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
+ED +VW E KDG FSVK C+N L D V FPW++IW+ F+P KV W
Sbjct: 35 LEDRMVWKESKDGIFSVKSCFNSL-----DHSSAVPFPWRIIWSTFVPTKVGFFAWEASW 89
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+V T+D L+ R + C C D ET + + IH + K +WD + + G+ W++ S
Sbjct: 90 GKVLTQDQLKRRGWNLANRCPLCCDEEETINHILIHCSKAKVLWDLLFSLFGVNWVLPFS 149
Query: 371 L 373
+
Sbjct: 150 V 150
>emb|CAN72177.1| hypothetical protein VITISV_012537 [Vitis vinifera]
Length = 491
Score = 96.7 bits (239), Expect = 6e-18
Identities = 45/121 (37%), Positives = 66/121 (54%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
+ED +VW E KDG FSVK C+N L D V FPW++IW+ F+P KV W
Sbjct: 341 LEDRMVWKETKDGIFSVKSCFNSL-----DHSSAVPFPWRIIWSSFVPSKVGFFAWEASW 395
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+V T+D L+ R ++ C C D ET + + IH K +WD + + G+ W++ S
Sbjct: 396 GKVLTQDQLKRRGWKLANRCPLCCDEEETINHILIHCPKAKVLWDLLFSLFGVNWVIPFS 455
Query: 371 L 373
+
Sbjct: 456 V 456
>emb|CAN81035.1| hypothetical protein VITISV_011008 [Vitis vinifera]
Length = 1016
Score = 88.2 bits (217), Expect = 2e-15
Identities = 41/121 (33%), Positives = 64/121 (52%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
+ED ++W K+G FSVK YN L D G V FPW++IW+ +P KV W
Sbjct: 874 MEDRVLWNASKNGIFSVKSLYNTL-----DSSGAVPFPWRIIWSPCVPTKVGFFAWEASW 928
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+V T+D L+ R + C C D ET + + IH + +W+ + + G+ W++ LS
Sbjct: 929 GKVLTQDQLKRRGWILANRCFLCCDDEETINHILIHCPKARVLWNLVFSLFGVNWVLLLS 988
Query: 371 L 373
+
Sbjct: 989 V 989
>emb|CAN75374.1| hypothetical protein VITISV_029152 [Vitis vinifera]
Length = 655
Score = 87.4 bits (215), Expect = 4e-15
Identities = 41/121 (33%), Positives = 62/121 (51%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
+ED +VW K+G FSVK YN L D G V FPW++IW+ +P KV W
Sbjct: 457 MEDRVVWNASKNGSFSVKSLYNTL-----DSGGAVPFPWRIIWSPCVPTKVGFFAWEASW 511
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+V T+D L+ R + C C D ET + + IH K +W+ + + W++ L+
Sbjct: 512 GKVLTQDHLKRRGWSLANKCFLCCDDEETINHILIHCPKAKVLWNLMFTMFRVNWVLQLT 571
Query: 371 L 373
+
Sbjct: 572 V 572
>emb|CAN79789.1| hypothetical protein VITISV_039779 [Vitis vinifera]
Length = 1566
Score = 85.1 bits (209), Expect = 2e-14
Identities = 40/121 (33%), Positives = 60/121 (49%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
+ED +VW K G FSVK YN L D G V FPW++IW+ +P KV W
Sbjct: 1368 MEDRVVWNASKSGSFSVKSLYNTL-----DSGGAVPFPWRIIWSPCVPTKVGFFAWEASW 1422
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+V T+D L+ R + C C D ET + + IH K +W+ + + W++ +
Sbjct: 1423 GKVLTQDHLKRRGWSLANRCFLCCDDEETINHILIHCPKAKVLWNLMFTMFRVNWVLQXT 1482
Query: 371 L 373
+
Sbjct: 1483 V 1483
>emb|CAN75589.1| hypothetical protein VITISV_042880 [Vitis vinifera]
Length = 816
Score = 84.0 bits (206), Expect = 4e-14
Identities = 39/121 (32%), Positives = 63/121 (52%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
+ED ++W K+G FSVK YN L D G FPW++IW+ +P KV W
Sbjct: 487 MEDRVLWNAYKNGIFSVKSLYNTL-----DSSGAXPFPWRIIWSPCVPTKVGFFAWEASW 541
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+V T+D L+ R + C C D ET + + IH + +W+ + + G+ W++ L+
Sbjct: 542 GKVLTQDQLKRRGWILANRCFLCCDEEETINHILIHCPKARVLWNLVFSLFGVNWVLPLT 601
Query: 371 L 373
+
Sbjct: 602 V 602
>emb|CAN75784.1| hypothetical protein VITISV_022492 [Vitis vinifera]
Length = 848
Score = 84.0 bits (206), Expect = 4e-14
Identities = 40/121 (33%), Positives = 63/121 (52%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
VED++ WTE K G+FSVK Y L + G+ L FP IWN + PK+S W
Sbjct: 650 VEDAVSWTETKSGKFSVKSLYIALEAGGSSL-----FPSSFIWNVNVQPKISFFAWEATW 704
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+ T DL++ R + C C++ E + L +H + + +WD + A G+ W++ S
Sbjct: 705 GKALTLDLVQKRGWALANRCFMCLEKEENINHLLLHCSRTRALWDLLFALFGVSWVLPFS 764
Query: 371 L 373
+
Sbjct: 765 V 765
>emb|CAN78294.1| hypothetical protein VITISV_035517 [Vitis vinifera]
Length = 350
Score = 82.4 bits (202), Expect = 1e-13
Identities = 39/120 (32%), Positives = 61/120 (50%)
Frame = +2
Query: 5 RYVEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGI 184
R ED +VWT K G FSVK Y+IL G+ + FP+ IW +PPKV+ W
Sbjct: 148 RVNEDKMVWTTSKSGVFSVKSLYSILEPGGSAM-----FPYVGIWKASVPPKVAFFAWEA 202
Query: 185 IRNRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMS 364
++ T D L+ R + C C+ ET L +H + + +W+ + + G+ W++S
Sbjct: 203 SWGKILTLDQLQRRGYSLANRCFLCLAEAETVDHLLLHCVMTRTLWNLLFSLFGVEWVLS 262
>ref|XP_006352585.1| PREDICTED: uncharacterized protein LOC102588593 [Solanum tuberosum]
Length = 255
Score = 82.0 bits (201), Expect = 2e-13
Identities = 40/115 (34%), Positives = 60/115 (52%)
Frame = +2
Query: 17 DSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIRNR 196
D+ +W K G FSVK CY L S + + +PWK+IW IP KVS W IR
Sbjct: 59 DTPIWKMHKKGIFSVKSCYWSLNS---NQAMEMEWPWKLIWKIKIPHKVSGFTWLAIRKA 115
Query: 197 VATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMM 361
T ++++ R I++ C C + E +S LF+H +W+ L +G+ W+M
Sbjct: 116 CLTHEVIQRRGIQICSRCFMCGEEAEVNSHLFLHCRTAANLWNMFLCILGVNWVM 170
>emb|CAN80245.1| hypothetical protein VITISV_031023 [Vitis vinifera]
Length = 1636
Score = 82.0 bits (201), Expect = 2e-13
Identities = 38/123 (30%), Positives = 63/123 (51%)
Frame = +2
Query: 5 RYVEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGI 184
R ED ++WT KDG FSV+ Y+++ G L FP + IW +PPKV+ W
Sbjct: 1437 REEEDRVIWTASKDGAFSVRSLYSMMEPGGLSL-----FPSERIWRTRVPPKVAFFAWEA 1491
Query: 185 IRNRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMS 364
+V T++ L+ R + C C+ ET L +H +++W+ + + GI W +S
Sbjct: 1492 SWGKVLTQEQLQRRGFSLANRCFLCLSEEETVDHLLLHCVKTRDLWNLLFSLFGISWTLS 1551
Query: 365 LSL 373
++
Sbjct: 1552 CTV 1554
>emb|CAN70922.1| hypothetical protein VITISV_016266 [Vitis vinifera]
Length = 945
Score = 81.6 bits (200), Expect = 2e-13
Identities = 37/121 (30%), Positives = 60/121 (49%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
VED + WTE K G+FSVK Y + + G+ FP +IWN ++ P +S W
Sbjct: 747 VEDRVSWTETKSGKFSVKSLYLAIEAGGS-----ARFPSSLIWNVYVQPNISFFAWEATW 801
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+ T D ++ R + C C++ ET L +H + K +WD + G+ W++ S
Sbjct: 802 GKALTLDKVQKRGWALANRCFLCLENEETIDHLLLHCSRTKVLWDLLFTVFGVSWVLPCS 861
Query: 371 L 373
+
Sbjct: 862 I 862
>emb|CAN74354.1| hypothetical protein VITISV_012842 [Vitis vinifera]
Length = 1240
Score = 81.6 bits (200), Expect = 2e-13
Identities = 39/121 (32%), Positives = 63/121 (52%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
VED +VWTE K G+FS K Y L + L FP IWN+++ PK+S W
Sbjct: 1042 VEDMVVWTETKSGKFSAKSLYLALEADCPVL-----FPSSCIWNRWVQPKISFFAWEAAW 1096
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
++ T DL++ R + C C++ ET L +H + + +W+ + G+ W++S S
Sbjct: 1097 DKALTLDLVQKRGWSLANRCFMCLEKEETIDHLLLHCSKTRALWELLFTLFGVSWVLSSS 1156
Query: 371 L 373
+
Sbjct: 1157 V 1157
>emb|CAN69899.1| hypothetical protein VITISV_029782 [Vitis vinifera]
Length = 760
Score = 81.6 bits (200), Expect = 2e-13
Identities = 39/121 (32%), Positives = 62/121 (51%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
VED++ WTE K G+FSVK Y L + G L FP IWN + PK+S W
Sbjct: 562 VEDTVSWTETKSGKFSVKSLYIALEAGGLSL-----FPSSFIWNVNVQPKISFFAWEATW 616
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+ T D+++ R + C C++ E + L +H + + +WD + A G+ W++ S
Sbjct: 617 GKALTLDMVQKRGWALANRCFMCLEKEENINHLLLHCSRTRALWDLLFALFGVSWVLPFS 676
Query: 371 L 373
+
Sbjct: 677 V 677
>emb|CAN69820.1| hypothetical protein VITISV_025709 [Vitis vinifera]
Length = 779
Score = 81.3 bits (199), Expect = 3e-13
Identities = 40/123 (32%), Positives = 62/123 (50%)
Frame = +2
Query: 5 RYVEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGI 184
R ED ++WT KDG FSV+ Y++L G L FP + IW +PPKV+ W
Sbjct: 515 REEEDRVIWTASKDGVFSVRSLYSMLEPGGLPL-----FPSERIWRARVPPKVAFFAWEA 569
Query: 185 IRNRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMS 364
+V T++ L+ R + C C+ ET L +H + +W+ + + GI W +S
Sbjct: 570 SWGKVLTQEQLQRRGFSLANRCFLCLSEEETVDHLLLHCIKTRVLWNLLFSLFGISWTLS 629
Query: 365 LSL 373
S+
Sbjct: 630 CSV 632
>emb|CAN70867.1| hypothetical protein VITISV_027610 [Vitis vinifera]
Length = 1177
Score = 80.9 bits (198), Expect = 3e-13
Identities = 38/121 (31%), Positives = 60/121 (49%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
VED + WTE K G+FSVK Y + + G+ FP +IWN + PK+S W
Sbjct: 829 VEDRVSWTETKSGKFSVKSLYLAIEAGGS-----ARFPSSLIWNANVQPKISFFAWEATW 883
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+ T D ++ R + C C++ ET L +H + K +WD + G+ W++ S
Sbjct: 884 GKALTLDKVQKRGWALANRCFLCLENEETIDHLLLHCSRTKVLWDLLFTIFGVSWVLPCS 943
Query: 371 L 373
+
Sbjct: 944 V 944
>emb|CAN64680.1| hypothetical protein VITISV_016601 [Vitis vinifera]
Length = 971
Score = 80.5 bits (197), Expect = 5e-13
Identities = 39/124 (31%), Positives = 63/124 (50%)
Frame = +2
Query: 2 YRYVEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWG 181
Y VED++ WTE K G+F VK Y L + G+ L FP IWN + PK+S W
Sbjct: 770 YEDVEDTVSWTETKSGKFLVKSLYIALEAGGSSL-----FPSSFIWNVNVQPKMSFFAWE 824
Query: 182 IIRNRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMM 361
+ T DL++ R + C C++ E + L +H + + +W+ + A G+ W++
Sbjct: 825 ATWGKALTLDLVQKRGWVLANRCFMCLEKEENINHLLLHCSRTRALWELLFALFGVSWVL 884
Query: 362 SLSL 373
S+
Sbjct: 885 PFSV 888
>emb|CAN71520.1| hypothetical protein VITISV_042316 [Vitis vinifera]
Length = 1202
Score = 80.5 bits (197), Expect = 5e-13
Identities = 39/121 (32%), Positives = 62/121 (51%)
Frame = +2
Query: 11 VEDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIR 190
VED++ WT K G+FSVK + L + G+ L FP IWN + PK+S W
Sbjct: 13 VEDTVSWTVTKSGKFSVKSLFIALEAGGSSL-----FPSSFIWNVNVQPKISFFAWEATW 67
Query: 191 NRVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLS 370
+ T DL++ R + C C++ E + L +H + + +WD + A G+ W+M S
Sbjct: 68 GKALTLDLVQKRGWALANRCFMCLEKEENINHLLLHCSRTRVLWDLLFALFGVSWVMPFS 127
Query: 371 L 373
+
Sbjct: 128 V 128
>emb|CAN68252.1| hypothetical protein VITISV_043921 [Vitis vinifera]
Length = 433
Score = 79.7 bits (195), Expect = 8e-13
Identities = 37/117 (31%), Positives = 59/117 (50%)
Frame = +2
Query: 14 EDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIRN 193
ED +VWT K G FSVK Y+ L G+ + FP+ IW +PPKV+ W
Sbjct: 188 EDKMVWTTSKSGVFSVKSLYSXLEPGGSAM-----FPYIGIWKASVPPKVAFFAWEASWG 242
Query: 194 RVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMS 364
++ T D L+ R + C C+ ET L +H + + +W+ + + G+ W++S
Sbjct: 243 KILTLDQLQRRGYSLANRCFLCLAEAETVDHLLLHCVMTRTLWNLLFSLFGVEWVLS 299
>ref|XP_009599592.1| PREDICTED: uncharacterized protein LOC104095219 [Nicotiana
tomentosiformis]
Length = 517
Score = 79.3 bits (194), Expect = 1e-12
Identities = 42/116 (36%), Positives = 57/116 (49%)
Frame = +2
Query: 14 EDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIRN 193
ED ++W K G FSVK Y+ + N +G +PWK W P KV W ++R
Sbjct: 382 EDVLMWKGHKLGVFSVKSAYHFFNTTTNPTQG---WPWKQTWRVRAPTKVLCFTWLVVRE 438
Query: 194 RVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMM 361
V T+D L R I++ C C E++S LFIH V ++W L GI W M
Sbjct: 439 AVLTQDNLIRRGIQLQARCSLCGKDGESASHLFIHCPVTTQLWHLYLHLAGISWTM 494
>emb|CAN68112.1| hypothetical protein VITISV_040983 [Vitis vinifera]
Length = 939
Score = 79.0 bits (193), Expect = 1e-12
Identities = 40/120 (33%), Positives = 60/120 (50%)
Frame = +2
Query: 14 EDSIVWTEDKDGQFSVKCCYNILRSRGNDLRGVVHFPWKMIWNKFIPPKVSVLVWGIIRN 193
ED ++W E KDG FSVK Y+IL SR V FP +IWN +P KV W
Sbjct: 742 EDCLLWKETKDGIFSVKSLYSILDSRSG-----VQFPINIIWNPCVPTKVGFFAWEAFWG 796
Query: 194 RVATRDLLRLRRIEVPENCVCCVDGMETSSQLFIHGAVEKEIWDRILAAMGIRWMMSLSL 373
+V T D L+ R + C C + E+ + I + + +W+ + A G+ W++ S+
Sbjct: 797 KVLTLDQLKKRGRCLANRCFLCCEEEESIDHILIQCSKARVLWELLFALFGVSWVLPYSV 856