BLASTX nr result
ID: Aconitum23_contig00034665
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum23_contig00034665
(858 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 411 e-112
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum] 374 e-101
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN... 276 2e-71
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 272 3e-70
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum] 271 3e-70
ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 271 6e-70
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 253 2e-64
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 252 3e-64
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 252 3e-64
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 252 3e-64
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 251 4e-64
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 247 7e-63
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 244 8e-62
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 243 1e-61
gb|ALG05133.1| cytochrome P450 [Sinopodophyllum hexandrum] 237 7e-60
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 232 2e-58
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 232 2e-58
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 231 4e-58
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 231 7e-58
emb|CDP15158.1| unnamed protein product [Coffea canephora] 227 7e-57
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 411 bits (1057), Expect = e-112
Identities = 207/285 (72%), Positives = 240/285 (84%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
LWLG QPTV+AST E+AMEILKTHDR+ S R IR SFRLK +I+YSL WSD TDYWK LR
Sbjct: 69 LWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLR 128
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RTEIFS KMLQAQ VRE+KV EL+D+LRSKEGQ VKIS+FVFGTLLN+LGN VFSK
Sbjct: 129 KIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSK 188
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
+VFVY IQNL REML IGAEPN+A+FYP LEE DLQG K++C F +++
Sbjct: 189 DVFVYGDETDKGG---IQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRVM 245
Query: 317 KLWEGVVEERKAKRHEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWVMAE 138
K+WEG V+ERKA R+E+++DMLDV+LAN F+DAQI+ LFLETFGPGSE+SS TIEWV+AE
Sbjct: 246 KMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAE 305
Query: 137 LIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
LIK+PKEMAKVRKEL+E VGTST+KES LPQLPYL ACIKEAMRL
Sbjct: 306 LIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRL 350
>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 374 bits (960), Expect = e-101
Identities = 185/285 (64%), Positives = 225/285 (78%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
LWLG QPT VAST E+AMEILKTHDR SGR +R SFRLK +I+YSL W+D ++YWK LR
Sbjct: 67 LWLGTQPTAVASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADCSEYWKLLR 126
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RTEIFS KMLQ Q VRE+KV EL+++L+ KEGQ VKIS+ VFGTLLN+LGN +FSK
Sbjct: 127 KIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSK 186
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
+VF Y +Q L +EML IGA PNLA+F+PI EE DLQG K+RC F++++
Sbjct: 187 DVFRYVGADGDK----MQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVM 242
Query: 317 KLWEGVVEERKAKRHEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWVMAE 138
K+W+G VEERK KR+E+ +DMLDV+L N F+DAQI+ +FLETFGPGSE+SS T EW +AE
Sbjct: 243 KMWKGTVEERKVKRNEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAE 302
Query: 137 LIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
LIKNP +AKVR+ELD+ VG STVKES LP LPYL ACIKE MRL
Sbjct: 303 LIKNPHILAKVRQELDQVVGKSTVKESDLPHLPYLQACIKETMRL 347
>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
Full=(S)-N-methylcoclaurine oxidase [C-O
phenol-coupling]; AltName: Full=CYPLXXX; AltName:
Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
cytochrome P-450 CYP80 [Berberis stolonifera]
Length = 487
Score = 276 bits (705), Expect = 2e-71
Identities = 137/286 (47%), Positives = 197/286 (68%), Gaps = 1/286 (0%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L G ++ AST E+AME+L+T+D+V SGR+ FR+K +I+YS+ WSD YWK+ R
Sbjct: 69 LKFGLHSSIFASTKEAAMEVLQTNDKVLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGR 128
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ TEIFS KMLQAQ + RE G L++++ +K G V++ ++FG LNVLG+ VFSK
Sbjct: 129 KILHTEIFSQKMLQAQEKNRERVAGNLVNFIMTKVGDVVELRSWLFGCALNVLGHVVFSK 188
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
+VF Y + L ML G + ++A ++P+L DL G KR+ F ++
Sbjct: 189 DVFEY---SDQSDEVGMDKLIHGMLMTGGDFDVASYFPVLARFDLHGLKRKMDEQFKLLI 245
Query: 317 KLWEGVVEERKAKRHEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWVMAE 138
K+WEG V R+A R+ + +DMLDV++AN F++ QI+ +F+ETFGPGS+++S IEW +A+
Sbjct: 246 KIWEGEVLARRANRNPEPKDMLDVLIANDFNEHQINAMFMETFGPGSDTNSNIIEWALAQ 305
Query: 137 LIKNPKEMAKVRKELDEAVG-TSTVKESHLPQLPYLHACIKEAMRL 3
LIKNP ++AK+R+ELD VG +STVKESH +LPYL AC+KE MRL
Sbjct: 306 LIKNPDKLAKLREELDRVVGRSSTVKESHFSELPYLQACVKETMRL 351
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 272 bits (695), Expect = 3e-70
Identities = 137/286 (47%), Positives = 190/286 (66%), Gaps = 1/286 (0%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L G +P VVAS+ +A+E+LK DR+ SGR S R+K YIE+S+ W+D TDYWK +R
Sbjct: 66 LKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWKMVR 125
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RTE+FS KML Q REEKV ELM +L KEG+ V + +FG++LN+LG +FSK
Sbjct: 126 KVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALIFSK 185
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
+ VY ++ + R+++ + A PNLAD YPIL D QG ++ A ++
Sbjct: 186 D--VYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVKRMN 243
Query: 317 KLWEGVVEERKAKRHEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWVMAE 138
+ W +V++R+ D L V+L +GFSD QID + LETFGPGS++S+ TIEW MAE
Sbjct: 244 ESWAAIVKQRRKNDDHSKNDFLQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTIEWAMAE 303
Query: 137 LIKNPKEMAKVRKELDEAVGTS-TVKESHLPQLPYLHACIKEAMRL 3
L++NP+++ KVR+ELD + S VKES LP LPYLHAC+KE +RL
Sbjct: 304 LLRNPEKLVKVREELDRVIRRSNNVKESDLPNLPYLHACVKETLRL 349
>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
Length = 346
Score = 271 bits (694), Expect = 3e-70
Identities = 135/214 (63%), Positives = 167/214 (78%)
Frame = -2
Query: 644 MLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSKNVFVYXXXXXX 465
MLQ Q VRE+KV EL+++L+ KEGQ VKIS+ VFGTLLN+LGN +FSK+VF Y
Sbjct: 1 MLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYVGADGD 60
Query: 464 XXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIVKLWEGVVEERK 285
+Q L +EML IGA PNLA+F+PI EE DLQG K+RC F++++K+W+G VEERK
Sbjct: 61 K----MQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERK 116
Query: 284 AKRHEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWVMAELIKNPKEMAKV 105
KR+E+ +DMLDV+L N F+DAQI+ +FLETFGPGSE+SS T EW +AELIKNP +AKV
Sbjct: 117 VKRNEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAKV 176
Query: 104 RKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
R+ELD+ VG STVKES LP LPYL ACIKE MRL
Sbjct: 177 RQELDQVVGKSTVKESDLPHLPYLQACIKETMRL 210
>ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 271 bits (692), Expect = 6e-70
Identities = 134/284 (47%), Positives = 188/284 (66%), Gaps = 2/284 (0%)
Frame = -2
Query: 848 GAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLRKLA 669
G + VVAS +AME+LK DRV SGR S R+K YIEYS+ W+D TDYWK +RK+
Sbjct: 69 GFENVVVASNHVAAMEVLKNQDRVLSGRFKANSVRVKGYIEYSMVWADCTDYWKMVRKIL 128
Query: 668 RTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSKNVF 489
RTE+FS KML VREEKV ELM +LR KEG+ V +FG +LN+LG ++SK+
Sbjct: 129 RTELFSTKMLDVHAHVREEKVSELMKFLRRKEGEEVNFVDVIFGCILNMLGALIYSKD-- 186
Query: 488 VYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPI-LEEHDLQGQKRRCANVFNKIVKL 312
VY ++ + R+++ + A PN+AD YPI + D QG ++ A ++ +
Sbjct: 187 VYDFEDKTDINLGMKGMIRQLMILAATPNIADLYPIFFDGSDFQGLRKESAACVKRMSES 246
Query: 311 WEGVVEERKAKRHEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWVMAELI 132
W ++ ER+ D+L V+L +GFSD QID +FLETFGPGS++S+ TIEW +AEL+
Sbjct: 247 WAAIINERRKNNDHTKNDLLQVLLDSGFSDPQIDAIFLETFGPGSDTSASTIEWALAELL 306
Query: 131 KNPKEMAKVRKELDEAVG-TSTVKESHLPQLPYLHACIKEAMRL 3
+NP+++ K+ +ELD +G +TVK+S LP LPYLHAC+KE +RL
Sbjct: 307 RNPEKLVKLHEELDRVIGRNNTVKDSDLPNLPYLHACVKETLRL 350
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 253 bits (645), Expect = 2e-64
Identities = 130/286 (45%), Positives = 192/286 (67%), Gaps = 1/286 (0%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L LG+Q VVAS+P +A E+LKTHD++ SGR + SFR+K+++E S+ WS+ D WK LR
Sbjct: 66 LKLGSQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLR 125
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RTE+F+ KM+++Q +RE K E++ +LRSKEG+ VKI + VFGTL+N+ GN +FSK
Sbjct: 126 KVCRTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSK 185
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
+VF +++ ++L +G N AD++PI+ ++DL GQ+R A+V +I
Sbjct: 186 DVF--DLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIY 243
Query: 317 KLWEGVVEERKA-KRHEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWVMA 141
+W ++ER+ K E D +DV+L G D +I+ L +E FG G+E+S+ TIEW +
Sbjct: 244 DVWGINLKERRGMKGSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAIT 303
Query: 140 ELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
EL K P ++K+R EL VG +TVKES LP LPYL A +KE +RL
Sbjct: 304 ELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRL 349
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 252 bits (643), Expect = 3e-64
Identities = 128/289 (44%), Positives = 185/289 (64%), Gaps = 4/289 (1%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L +G + VVAST +A EILKTHDR+ S R + SFR+K ++E S+ WSD T+ WK LR
Sbjct: 66 LKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLR 125
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RTE+F+ KM+++Q VRE+K E+++YL K+G+ VKI + +FGTL+N+ GN +FS+
Sbjct: 126 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 185
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
N+F + + ML +G N AD++P+L + DL GQ++ A I
Sbjct: 186 NIF--ELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIY 243
Query: 317 KLWEGVVEERKAKR----HEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEW 150
+W +++ERK + ++ D +DV L +G +D QI+ L +E FG G+E+S+ TIEW
Sbjct: 244 AIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEW 303
Query: 149 VMAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
M EL KNPK AK+R EL VG +VKES P LPYL A +KE +RL
Sbjct: 304 AMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRL 352
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
isozyme 2; AltName: Full=Cytochrome P450 80B2 [Coptis
japonica] gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 252 bits (643), Expect = 3e-64
Identities = 130/286 (45%), Positives = 190/286 (66%), Gaps = 1/286 (0%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L LG+Q VVAS+P +A EILKTHD++ SGR + SFR+K+++E S+ WS+ D WK LR
Sbjct: 66 LKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLR 125
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RTE+F+ KM+++Q +RE K E++ +LR KEG+ VKI + VFGTL+N+ GN +FSK
Sbjct: 126 KVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSK 185
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
+VF +++ ++L +G N AD++PI+ + DL GQ+R A V +I
Sbjct: 186 DVF--DLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIY 243
Query: 317 KLWEGVVEERK-AKRHEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWVMA 141
+W +++ER+ K E D +DV+L G D +I+ L +E FG G+E+S+ TIEW +
Sbjct: 244 DVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAIT 303
Query: 140 ELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
EL K P ++K+R EL VG +TVKES LP LPYL A +KE +RL
Sbjct: 304 ELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRL 349
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2
gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 488
Score = 252 bits (643), Expect = 3e-64
Identities = 129/289 (44%), Positives = 186/289 (64%), Gaps = 4/289 (1%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L +G + VVAST +A EILKTHDR+ S R + SFR+K ++E S+ WSD T+ WK LR
Sbjct: 66 LKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLR 125
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RTE+F+ KM+++Q VRE+K E+++YL K+G+ VKI + +FGTL+N+ GN +FS+
Sbjct: 126 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 185
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
N+F + + ML +G N AD++P+L + DL GQ++ A I
Sbjct: 186 NIF--ELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIY 243
Query: 317 KLWEGVVEERKAKR----HEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEW 150
+W +++ERK + ++ D +DV L +G +D QI+ L +E FG G+E+S+ TIEW
Sbjct: 244 AIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEW 303
Query: 149 VMAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
M EL KNPK AK+R EL VG +VKES P LPYL A +KE +RL
Sbjct: 304 AMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRL 352
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1
gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 487
Score = 251 bits (642), Expect = 4e-64
Identities = 127/289 (43%), Positives = 185/289 (64%), Gaps = 4/289 (1%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L +G + VVAST +A EILKTHDR+ S R + SFR+K ++E S+ WSD T+ WK LR
Sbjct: 65 LKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLR 124
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RTE+F+ KM+++Q VRE+K E+++YL K+G+ VKI + +FGTL+N+ GN +FS+
Sbjct: 125 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 184
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
N+F + + ML +G N AD++P+L + DL GQ++ A I
Sbjct: 185 NIF--ELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIY 242
Query: 317 KLWEGVVEERKAKR----HEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEW 150
+W +++ERK + ++ D +DV L +G +D QI+ L +E FG G+E+S+ TIEW
Sbjct: 243 AIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEW 302
Query: 149 VMAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
M EL KNPK AK+R E+ VG +VKES P LPYL A +KE +RL
Sbjct: 303 AMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRL 351
>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3
gi|6176562|gb|AAF05621.1|AF191772_1
(S)-N-methylcoclaurine 3'-hydroxylase [Papaver
somniferum]
Length = 481
Score = 247 bits (631), Expect = 7e-63
Identities = 129/288 (44%), Positives = 191/288 (66%), Gaps = 3/288 (1%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L LG++ VVASTP +A EILKTHDRV SGR + SFR+K+++E S+ WS+ + WK+LR
Sbjct: 60 LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLR 119
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RTE+F+ KM+++Q VRE K E+++YL+ G VKI++ VFGTL+N+ GN +FS+
Sbjct: 120 KVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 179
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
N+F ++L R ML +G N AD++P L + DL GQ + A+ I
Sbjct: 180 NIFKLGDESSGSVEMK-EHLWR-MLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIY 237
Query: 317 KLWEGVVEERK-AKRHEQAE--DMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWV 147
+W +++ERK AK+H ++ D ++++L +G D QI+ L +E FG G+E+S+ TIEW
Sbjct: 238 SVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWA 297
Query: 146 MAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
++EL KNP+ A +R EL VG VKES +P +PYL A +KE +RL
Sbjct: 298 LSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRL 345
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 244 bits (622), Expect = 8e-62
Identities = 127/288 (44%), Positives = 190/288 (65%), Gaps = 3/288 (1%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L LG++ VVASTP +A EILKTHDRV SGR + SFR+K+++E S+ WS+ + WK+LR
Sbjct: 60 LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLR 119
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RT++F+ KM+++Q VRE K E+++YL+ G VKI++ VFGTL+N+ GN +FS+
Sbjct: 120 KVCRTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQ 179
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
N+F ++L R ML +G N AD++P L + DL GQ + A+ I
Sbjct: 180 NIFKLGDESSGSVEMK-EHLWR-MLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIY 237
Query: 317 KLWEGVVEERK-AKRHEQAE--DMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWV 147
+W +++E K AK+H ++ D ++++L +G D QI+ L +E FG G+E+S+ TIEW
Sbjct: 238 SVWGAMLKESKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWA 297
Query: 146 MAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
++EL KNP+ A +R EL VG VKES +P +PYL A +KE +RL
Sbjct: 298 LSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRL 345
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 243 bits (621), Expect = 1e-61
Identities = 126/288 (43%), Positives = 189/288 (65%), Gaps = 3/288 (1%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L LG++ VVASTP +A EILKTHDRV SGR + SFR+K+++E S+ WS+ + WK+LR
Sbjct: 65 LKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLR 124
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ R E+F+ KM+++Q +RE K E++++L+ +G VKI + VFGTL+N+ GN +FS+
Sbjct: 125 KVCRAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQ 184
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
N+F ++L R ML +G N AD++P L DL GQ++ A+ I
Sbjct: 185 NIFKLGDESSGSVEMK-EHLWR-MLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIY 242
Query: 317 KLWEGVVEERK-AKRHEQAE--DMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWV 147
+W +++ERK AK H ++ D ++++L +G D QI+ L +E FG G+E+S+ TIEW
Sbjct: 243 SVWGAMLKERKIAKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWA 302
Query: 146 MAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
++EL KNP+ A +R EL VG VKES +P +PYL A +KE +RL
Sbjct: 303 LSELTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKETLRL 350
>gb|ALG05133.1| cytochrome P450 [Sinopodophyllum hexandrum]
Length = 494
Score = 237 bits (605), Expect = 7e-60
Identities = 128/294 (43%), Positives = 181/294 (61%), Gaps = 9/294 (3%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYI--EYSLAWSDRTDYWKR 684
L LGAQ VV STP +A EILK HDRV SGR + + K ++SLAW+D TD K
Sbjct: 71 LRLGAQLLVVGSTPAAAAEILKAHDRVLSGRAMPNASPYKNPAGRQFSLAWADCTDQRKY 130
Query: 683 LRKLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVF 504
L + R+E+FS K +++Q +REEK+ +L+++L KEG+ V I VF T+ N L N
Sbjct: 131 LHTICRSELFSIKAIESQSNLREEKISKLIEFLGRKEGELVNIGDVVFATVFNTLSNIFV 190
Query: 503 SKNVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNK 324
S++ + ++ L R + GA PNLADFYPIL DLQG +++ +F K
Sbjct: 191 SQD---FITLEGHKDGDGMKELVRSFVVAGATPNLADFYPILSSFDLQGLRKKSIGLFAK 247
Query: 323 IVKLWEGVVEERKA-------KRHEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSS 165
I LWE +++ER+ KRH D LD++LANGF+D Q+++L LE G+++++
Sbjct: 248 ICSLWESIIKERRQDKSSGVLKRHT---DFLDILLANGFTDDQMNVLLLELLLAGADTTT 304
Query: 164 VTIEWVMAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
T EW MAEL+KN K M V KE + + V+ES LP LPYLH+C+KEA+R+
Sbjct: 305 STTEWAMAELLKNEKVMKNVCKEFEREIKEDVVRESDLPHLPYLHSCVKEALRI 358
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 232 bits (592), Expect = 2e-58
Identities = 126/290 (43%), Positives = 185/290 (63%), Gaps = 5/290 (1%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLK--QYIEYSLAWS-DRTDYWK 687
L LG Q VVAS+P +AME+LKTHDR SGR + +S +K + S+ ++ D WK
Sbjct: 615 LRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWK 674
Query: 686 RLRKLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAV 507
LR + R E+FS K +++Q +RE KV EL+++L +KEG+ VK+ VF T+ N+L N
Sbjct: 675 NLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKF 734
Query: 506 FSKNVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFN 327
FS ++ + +++L + GA PNL+D+YPIL D+QG R+ +F
Sbjct: 735 FSMDLCDFEDEGRVGGA--LKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFE 792
Query: 326 KIVKLWEGVVEERKAKRHEQAE--DMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIE 153
+I WE +++ER+ +R ++ D L+ +L GF D QI+ + LE F G+E+SS+T+E
Sbjct: 793 RIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVE 852
Query: 152 WVMAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
W MAELI+N M K+R EL + VG S V+ESHLP+LPYL AC+KEA+RL
Sbjct: 853 WAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRL 902
Score = 224 bits (572), Expect = 5e-56
Identities = 119/290 (41%), Positives = 183/290 (63%), Gaps = 5/290 (1%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLK--QYIEYSLAWSDR-TDYWK 687
L LG Q VVAS+P +A+E+LKTHDR SGR + +S +K + SLA++ T+ WK
Sbjct: 1120 LRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFAKECTNNWK 1179
Query: 686 RLRKLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAV 507
LR + RTE+FS K +++ +RE KV EL+++L +KEG+ VK+ VF T+ N+L N
Sbjct: 1180 NLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTF 1239
Query: 506 FSKNVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFN 327
FS ++ + +++ +GA PNL+DFYPIL+ +L G K++
Sbjct: 1240 FSMDLCDFEREG-------LKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALG 1292
Query: 326 KIVKLWEGVVEERKAKRHEQAE--DMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIE 153
+I+ WEG ++ER+ +++ + D+L+ L F D QI+ + LE F G+++S++TIE
Sbjct: 1293 RILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIE 1352
Query: 152 WVMAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
W + +LI+NP M K+R EL + +G S V+ESHLP LPYL AC+KE +RL
Sbjct: 1353 WAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACVKETLRL 1402
Score = 210 bits (535), Expect = 9e-52
Identities = 117/291 (40%), Positives = 176/291 (60%), Gaps = 6/291 (2%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQ----YIEYSLAWSDRTDYW 690
L LG Q VVAS+ +AME+LKTHDR SGR + T+ + ++ + A +D W
Sbjct: 75 LRLGTQIMVVASSSAAAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKVCNSD-W 133
Query: 689 KRLRKLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNA 510
+ L+ + R E+FS K ++++ +RE KV EL+++L KEG+ VK+ V+ T+ N+L N
Sbjct: 134 RNLKAICRMELFSGKAMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSNK 193
Query: 509 VFSKNVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVF 330
FS + + +++L E +GA N+ DFYPIL D+QG +++ +F
Sbjct: 194 FFSIDFSDFEGRDVRGVL--LKDLLNENAELGAT-NILDFYPILGGLDIQGIRKKLKEIF 250
Query: 329 NKIVKLWEGVVEERKAKRHEQAE--DMLDVVLANGFSDAQIDILFLETFGPGSESSSVTI 156
+I WE +++ER+ +R + D LD +L GF D QI+ + +E F G E+SS+T+
Sbjct: 251 RRIPTTWEDILKERRKQRIHGSSHGDFLDALLETGFEDDQINHVIMELFFAGPETSSLTV 310
Query: 155 EWVMAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
EW MAELIKN M K+ EL + +G S V+ESHLP LPYL AC+KE +RL
Sbjct: 311 EWAMAELIKNQDAMHKLCNELTQIIGESPVRESHLPHLPYLQACVKETLRL 361
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 232 bits (592), Expect = 2e-58
Identities = 126/290 (43%), Positives = 185/290 (63%), Gaps = 5/290 (1%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLK--QYIEYSLAWS-DRTDYWK 687
L LG Q VVAS+P +AME+LKTHDR SGR + +S +K + S+ ++ D WK
Sbjct: 75 LRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWK 134
Query: 686 RLRKLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAV 507
LR + R E+FS K +++Q +RE KV EL+++L +KEG+ VK+ VF T+ N+L N
Sbjct: 135 NLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKF 194
Query: 506 FSKNVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFN 327
FS ++ + +++L + GA PNL+D+YPIL D+QG R+ +F
Sbjct: 195 FSMDLCDFEDEGRVGGA--LKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFE 252
Query: 326 KIVKLWEGVVEERKAKRHEQAE--DMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIE 153
+I WE +++ER+ +R ++ D L+ +L GF D QI+ + LE F G+E+SS+T+E
Sbjct: 253 RIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVE 312
Query: 152 WVMAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
W MAELI+N M K+R EL + VG S V+ESHLP+LPYL AC+KEA+RL
Sbjct: 313 WAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRL 362
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 231 bits (590), Expect = 4e-58
Identities = 126/290 (43%), Positives = 184/290 (63%), Gaps = 5/290 (1%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLK--QYIEYSLAWS-DRTDYWK 687
L LG Q VVAS+P +AME+LKTHDR SGR + S +K + S+ ++ D WK
Sbjct: 75 LRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHLSIVFAKDCNTNWK 134
Query: 686 RLRKLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAV 507
LR + R E+FS K +++Q +RE KV EL+++L +KEG+ VK+ VF T+ N+L N
Sbjct: 135 NLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKF 194
Query: 506 FSKNVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFN 327
FS ++ + +++L + GA PNL+D+YPIL D+QG R+ +F
Sbjct: 195 FSMDLCDFEDEGRVGGA--LKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFE 252
Query: 326 KIVKLWEGVVEERKAKRHEQAE--DMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIE 153
+I WE +++ER+ +R ++ D L+ +L GF D QI+ + LE F G+E+SS+T+E
Sbjct: 253 RIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVE 312
Query: 152 WVMAELIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
W MAELI+N M K+R EL + VG S V+ESHLP+LPYL AC+KEA+RL
Sbjct: 313 WAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVKEALRL 362
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 231 bits (588), Expect = 7e-58
Identities = 119/285 (41%), Positives = 184/285 (64%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRIRTSFRLKQYIEYSLAWSDRTDYWKRLR 678
L LG+Q VVAS+P +A EILKT D++ SGR + SFR+ +++ S+ WS+ + WK LR
Sbjct: 67 LKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNENWKLLR 126
Query: 677 KLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAVFSK 498
K+ RTE+FS KM+++Q +RE K +++ +LR KE Q VKI + VF TL+N+ GN +FSK
Sbjct: 127 KVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLIFSK 186
Query: 497 NVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFNKIV 318
+VF +++ ++L +G N AD++PI+ + DL GQ+R A V +I
Sbjct: 187 DVF--DLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVLKQIY 244
Query: 317 KLWEGVVEERKAKRHEQAEDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTIEWVMAE 138
+W +++ER++ + D +V+L G D +I+ L +E FG G+E+++ TIEW + E
Sbjct: 245 DVWGVMLKERRSITGHRENDFANVLLNAGLDDQKINALLMELFGAGTETTASTIEWAITE 304
Query: 137 LIKNPKEMAKVRKELDEAVGTSTVKESHLPQLPYLHACIKEAMRL 3
L N + ++K+R EL VG TVKES++P LPYL A +KE +RL
Sbjct: 305 LTNNLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKETLRL 349
>emb|CDP15158.1| unnamed protein product [Coffea canephora]
Length = 496
Score = 227 bits (579), Expect = 7e-57
Identities = 125/293 (42%), Positives = 184/293 (62%), Gaps = 8/293 (2%)
Frame = -2
Query: 857 LWLGAQPTVVASTPESAMEILKTHDRVCSGRRI-RTSFRLKQYIEY-SLAWS-DRTDYWK 687
L LG+Q VV STP +A EILKTHDR+ SGR + S+ + Y S+ W+ + TD WK
Sbjct: 71 LRLGSQLVVVGSTPAAATEILKTHDRILSGRHVPHVSYAKSPLMNYVSVGWTYECTDQWK 130
Query: 686 RLRKLARTEIFSAKMLQAQRRVREEKVGELMDYLRSKEGQAVKISKFVFGTLLNVLGNAV 507
LR L ++EI AK+++ Q +RE+K EL+ +L SKEGQ +KI++ VF ++ N LG
Sbjct: 131 FLRTLCKSEILGAKVIENQSHLREQKANELVQFLVSKEGQRIKIAEVVFVSVFNFLGQIF 190
Query: 506 FSKNVFVYXXXXXXXXXXDIQNLTREMLTIGAEPNLADFYPILEEHDLQGQKRRCANVFN 327
FSK+ Y + L RE++ + PN++D YP+L DLQ ++ + N
Sbjct: 191 FSKDFLSY---DEVENGGGMSELIREVMELWTAPNISDLYPVLGGLDLQRLSKKASVCHN 247
Query: 326 KIVKLWEGVVEERKAKRHEQA---EDMLDVVLANGFSDAQIDILFLETFGPGSESSSVTI 156
KI W+ ++ ER+ K+++ + +D LDV+L N FSD QI+ L LE F GS++S+ T+
Sbjct: 248 KICSAWQEIIRERRGKKYQDSTRQKDFLDVLLQNDFSDDQINYLHLELFAAGSDTSTSTV 307
Query: 155 EWVMAELIKNPKEMAKVRKELD--EAVGTSTVKESHLPQLPYLHACIKEAMRL 3
EW MAEL++NPK + +R+ELD E + +KES L +LPYL AC+KE +RL
Sbjct: 308 EWAMAELLRNPKFLDMIRQELDQIEYSVENVIKESDLTRLPYLQACVKEILRL 360