BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum23_contig00004919
         (798 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ALG05133.1| cytochrome P450 [Sinopodophyllum hexandrum]            259   1e-66
ref|XP_002283461.2| PREDICTED: probable (S)-N-methylcoclaurine 3...   253   1e-64
emb|CBI34714.3| unnamed protein product [Vitis vinifera]              253   1e-64
ref|XP_002283415.2| PREDICTED: probable (S)-N-methylcoclaurine 3...   251   4e-64
emb|CBI34715.3| unnamed protein product [Vitis vinifera]              250   9e-64
ref|XP_011030059.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   231   3e-58
ref|XP_011030058.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   231   3e-58
ref|XP_010112571.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   229   1e-57
ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   229   1e-57
emb|CBI31830.3| unnamed protein product [Vitis vinifera]              229   1e-57
ref|XP_010112570.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   229   2e-57
ref|XP_002318834.2| hypothetical protein POPTR_0012s13620g [Popu...   228   3e-57
ref|XP_010662049.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   228   5e-57
emb|CBI34743.3| unnamed protein product [Vitis vinifera]              228   5e-57
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   227   6e-57
ref|XP_002509713.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   227   8e-57
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ...   226   1e-56
ref|XP_011030061.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   226   1e-56
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   226   1e-56
ref|XP_002274586.2| PREDICTED: probable (S)-N-methylcoclaurine 3...   226   1e-56

>gb|ALG05133.1| cytochrome P450 [Sinopodophyllum hexandrum]
          Length = 494

 Score =  259 bits (663), Expect = 1e-66
 Identities = 124/202 (61%), Positives = 156/202 (77%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           ELL AGAD +TST EWA+AEL+K+ + M+ V  E E    + VV+ESDLPHLPYLHS VK
Sbjct: 294 ELLLAGADTTTSTTEWAMAELLKNEKVMKNVCKEFEREIKEDVVRESDLPHLPYLHSCVK 353

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           E               A ETCKV+NYTIPKD++VFVNVWAIGRDPK W+DPL FKPERF 
Sbjct: 354 EALRIHPPAPFLLPRRAIETCKVMNYTIPKDSQVFVNVWAIGRDPKIWDDPLAFKPERFL 413

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           DS ++ +SN+  +IPFG+GRR+CPG+PMA KQ+  +LA LI+SFDW LP+D+NP+ +LDM
Sbjct: 414 DSKVELHSNDFGWIPFGSGRRMCPGIPMATKQLGFVLASLIKSFDWSLPYDMNPS-ELDM 472

Query: 257 EEKFGLALQKEKPLVLIPKLRK 192
            EKFG+ LQKEKPLVLIP++++
Sbjct: 473 AEKFGITLQKEKPLVLIPRVKQ 494


>ref|XP_002283461.2| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 540

 Score =  253 bits (646), Expect = 1e-64
 Identities = 123/202 (60%), Positives = 152/202 (75%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           ELL AGAD S+ST+EWA+AEL+K PE+M++V  EL    SD + K SDLPHLPYL + VK
Sbjct: 340 ELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVK 399

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              AS +C+V+NYTIPKD++++VN WAIGRDP  WEDPL FKPERF 
Sbjct: 400 ETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFL 459

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +DF  NN  +IPFGAGRRICPGLPMAA+ +PLILA L   FDW LP+   P D+LDM
Sbjct: 460 NSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTP-DELDM 518

Query: 257 EEKFGLALQKEKPLVLIPKLRK 192
            +KFG+ALQKE+PL++IPK+RK
Sbjct: 519 NDKFGIALQKEQPLLIIPKVRK 540


>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
          Length = 936

 Score =  253 bits (646), Expect = 1e-64
 Identities = 123/202 (60%), Positives = 152/202 (75%)
 Frame = -2

Query: 797  ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
            ELL AGAD S+ST+EWA+AEL+K PE+M++V  EL    SD + K SDLPHLPYL + VK
Sbjct: 736  ELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVK 795

Query: 617  ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
            ET              AS +C+V+NYTIPKD++++VN WAIGRDP  WEDPL FKPERF 
Sbjct: 796  ETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFL 855

Query: 437  DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
            +S +DF  NN  +IPFGAGRRICPGLPMAA+ +PLILA L   FDW LP+   P D+LDM
Sbjct: 856  NSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTP-DELDM 914

Query: 257  EEKFGLALQKEKPLVLIPKLRK 192
             +KFG+ALQKE+PL++IPK+RK
Sbjct: 915  NDKFGIALQKEQPLLIIPKVRK 936


>ref|XP_002283415.2| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 540

 Score =  251 bits (641), Expect = 4e-64
 Identities = 122/202 (60%), Positives = 151/202 (74%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           ELL AGAD S+ST+EWA+AEL+K PE+M++V  EL    SD + K SDLPHLPYL + VK
Sbjct: 340 ELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVK 399

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              AS +C+V+NYTIPKD++++VN WAIGRDP  WEDPL FKPERF 
Sbjct: 400 ETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFL 459

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +DF  NN  +IPFGAGRRICPGLPMAA+ +PLILA L   FDW LP+   P D+LDM
Sbjct: 460 NSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTP-DELDM 518

Query: 257 EEKFGLALQKEKPLVLIPKLRK 192
            +KFG+ LQKE+PL++IPK+RK
Sbjct: 519 NDKFGVTLQKEQPLLIIPKVRK 540


>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
          Length = 1076

 Score =  250 bits (638), Expect = 9e-64
 Identities = 122/202 (60%), Positives = 150/202 (74%)
 Frame = -2

Query: 797  ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
            ELL AGAD S+ST+EWA+AEL+K PE+M++V  EL    SD + K SDLPHLPYL + VK
Sbjct: 867  ELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVK 926

Query: 617  ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
            ET              AS +C+V+NYTIPKD++++VN WAIGRDP  WEDPL FKPERF 
Sbjct: 927  ETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFL 986

Query: 437  DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
            +S +DF  NN  +IPFGAGRRICPGLPMAA+ +PLILA L   FDW LP+   P D+LDM
Sbjct: 987  NSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLPNGTTP-DELDM 1045

Query: 257  EEKFGLALQKEKPLVLIPKLRK 192
             +KFG+ LQKE+PL++IPK RK
Sbjct: 1046 NDKFGVTLQKEQPLLIIPKKRK 1067


>ref|XP_011030059.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Populus euphratica]
          Length = 505

 Score =  231 bits (590), Expect = 3e-58
 Identities = 115/202 (56%), Positives = 147/202 (72%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           ELL A +D+S+STVEWA+AELM++P+AM+++R EL   T + ++ ES L   PYLH  VK
Sbjct: 305 ELLAAVSDSSSSTVEWAMAELMRNPQAMKQLREELAGETPEDLITESSLAKFPYLHLCVK 364

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A+E C+VL+YTIPKDT+V VNVWAI RDP  WEDP  FKPERF 
Sbjct: 365 ETLRLHPPAPLLIPHRATEDCQVLDYTIPKDTQVLVNVWAIARDPANWEDPSCFKPERFL 424

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +D+  N+  Y+PFG+GRRIC GLPMA K+V L LA LI  FDW LP+++ P D+LDM
Sbjct: 425 NSDLDYKGNHFEYLPFGSGRRICAGLPMAVKKVQLALANLIHGFDWSLPNNMLP-DELDM 483

Query: 257 EEKFGLALQKEKPLVLIPKLRK 192
           +EK+G+ L KE+PL LIPKLRK
Sbjct: 484 DEKYGITLMKEQPLKLIPKLRK 505


>ref|XP_011030058.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Populus euphratica]
          Length = 505

 Score =  231 bits (590), Expect = 3e-58
 Identities = 115/202 (56%), Positives = 147/202 (72%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           ELL A +D+S+STVEWA+AELM++P+AM+++R EL   T + ++ ES L   PYLH  VK
Sbjct: 305 ELLAAVSDSSSSTVEWAMAELMRNPQAMKQLREELAGETPEDLITESSLAKFPYLHLCVK 364

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A+E C+VL+YTIPKDT+V VNVWAI RDP  WEDP  FKPERF 
Sbjct: 365 ETLRLHPPAPLLIPHRATEDCQVLDYTIPKDTQVLVNVWAIARDPANWEDPSCFKPERFL 424

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +D+  N+  Y+PFG+GRRIC GLPMA K+V L LA LI  FDW LP+++ P D+LDM
Sbjct: 425 NSDLDYKGNHFEYLPFGSGRRICAGLPMAVKKVQLALANLIHGFDWSLPNNMLP-DELDM 483

Query: 257 EEKFGLALQKEKPLVLIPKLRK 192
           +EK+G+ L KE+PL LIPKLRK
Sbjct: 484 DEKYGITLMKEQPLKLIPKLRK 505


>ref|XP_010112571.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Morus notabilis]
           gi|587947841|gb|EXC34118.1| (S)-N-methylcoclaurine
           3'-hydroxylase isozyme 2 [Morus notabilis]
          Length = 499

 Score =  229 bits (585), Expect = 1e-57
 Identities = 111/202 (54%), Positives = 146/202 (72%), Gaps = 1/202 (0%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFEL-ETVTSDKVVKESDLPHLPYLHSVV 621
           EL+ AG D S+ST+EW L EL+K+P+ M+ V+ EL   +    +V+ES LP LPYL + V
Sbjct: 298 ELITAGTDTSSSTIEWTLTELIKNPKCMKIVKAELAREIVDQDMVRESHLPKLPYLQACV 357

Query: 620 KETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERF 441
           KET              A E+C+VLNYTIPKD+++ VNVWAIGRDP  W+DPLTFKPERF
Sbjct: 358 KETLRLHPPAPFLLPHRAVESCQVLNYTIPKDSQLLVNVWAIGRDPMYWKDPLTFKPERF 417

Query: 440 HDSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLD 261
            +S +DF  N+  ++PFGAGRRICPGLPMAAK VPL++A LI   DW LP+  +P D ++
Sbjct: 418 LESSLDFKGNDFEFLPFGAGRRICPGLPMAAKHVPLVVASLIHFLDWSLPYGKDPLD-MN 476

Query: 260 MEEKFGLALQKEKPLVLIPKLR 195
           M EK+G+ L KE+PL+LIPK++
Sbjct: 477 MSEKYGITLIKEEPLILIPKVK 498


>ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 493

 Score =  229 bits (585), Expect = 1e-57
 Identities = 108/202 (53%), Positives = 146/202 (72%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           EL  AGAD ST T+EWA+ +L+++P+ M ++R EL  +  +  V+ES LPHLPYL + VK
Sbjct: 293 ELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACVK 352

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A ETC+V+ YTIPKD++VFVN+WA+GRDPK W+DPL+F PERF 
Sbjct: 353 ETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERFL 412

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           DS ++F  N+  YIPFGAGRRICPG+ + A+QVPL+LA L+  FDW LP +++ + Q+DM
Sbjct: 413 DSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMD-SAQIDM 471

Query: 257 EEKFGLALQKEKPLVLIPKLRK 192
           EE   + L+KE PL L+PK+RK
Sbjct: 472 EEWLVITLRKENPLRLVPKVRK 493


>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
          Length = 1538

 Score =  229 bits (585), Expect = 1e-57
 Identities = 108/202 (53%), Positives = 146/202 (72%)
 Frame = -2

Query: 797  ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
            EL  AGAD ST T+EWA+ +L+++P+ M ++R EL  +  +  V+ES LPHLPYL + VK
Sbjct: 1338 ELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLPYLQACVK 1397

Query: 617  ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
            ET              A ETC+V+ YTIPKD++VFVN+WA+GRDPK W+DPL+F PERF 
Sbjct: 1398 ETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLSFTPERFL 1457

Query: 437  DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
            DS ++F  N+  YIPFGAGRRICPG+ + A+QVPL+LA L+  FDW LP +++ + Q+DM
Sbjct: 1458 DSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMD-SAQIDM 1516

Query: 257  EEKFGLALQKEKPLVLIPKLRK 192
            EE   + L+KE PL L+PK+RK
Sbjct: 1517 EEWLVITLRKENPLRLVPKVRK 1538



 Score =  220 bits (561), Expect = 8e-55
 Identities = 106/202 (52%), Positives = 144/202 (71%)
 Frame = -2

Query: 797  ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
            EL  AGA+ S+ TVEWA+AEL+++ +AM ++R EL  +  +  V+ES LP LPYL + VK
Sbjct: 838  ELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLPYLQACVK 897

Query: 617  ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
            E               A+ETC+V+ YTIPKD+++FVN+WA+ RDPK W+DPL+FKPERF 
Sbjct: 898  EALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLSFKPERFL 957

Query: 437  DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
            DS +DF  N+  YIPFGAGRRICPGL +  +QVPLILA  +  F W LP +++ + QLDM
Sbjct: 958  DSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMD-SAQLDM 1016

Query: 257  EEKFGLALQKEKPLVLIPKLRK 192
            EE   + L+KE+PL L+P++RK
Sbjct: 1017 EEWLVITLRKEQPLRLVPRVRK 1038



 Score =  219 bits (559), Expect = 1e-54
 Identities = 104/199 (52%), Positives = 144/199 (72%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           EL FAG + S+ TVEWA+AEL+K+ +AM ++  EL  +  +  V+ES LPHLPYL + VK
Sbjct: 297 ELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQIIGESPVRESHLPHLPYLQACVK 356

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A+ETC+++ YTIPKD+ +FVN+WA+GRDP  WEDPL+FKPERF 
Sbjct: 357 ETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWEDPLSFKPERFL 416

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           DS ++F  N+  YIPFGAGRR+CPG+P+AA+ VP+ILA  ++ FDW  P D++   ++DM
Sbjct: 417 DSKLEFKGNDFEYIPFGAGRRMCPGMPLAARLVPMILATFVRLFDWSTPGDMD-FAEIDM 475

Query: 257 EEKFGLALQKEKPLVLIPK 201
           EE+F + L+KE+PL L+ K
Sbjct: 476 EERFVITLRKEQPLRLLKK 494


>ref|XP_010112570.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Morus notabilis]
           gi|587947840|gb|EXC34117.1| (S)-N-methylcoclaurine
           3'-hydroxylase isozyme 2 [Morus notabilis]
          Length = 511

 Score =  229 bits (584), Expect = 2e-57
 Identities = 108/203 (53%), Positives = 145/203 (71%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           ELL AG+D+S +T+EW + EL++ P  M+ V+ EL    S  +V ESDLP  PYL +  K
Sbjct: 303 ELLLAGSDSSAATIEWIMVELIRSPRCMKIVQEELAREISQGMVTESDLPKFPYLQACFK 362

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A E+C+V+NYTIPKD++V VNVWAIGRDP+ W+DPL FKPERF 
Sbjct: 363 ETLRLHPPAPLLIPRRALESCQVMNYTIPKDSKVQVNVWAIGRDPEYWKDPLVFKPERFL 422

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           DS ++F  N+  Y+PFG+GRR+CPG+PMA+KQVPL+LA L+  FDW LP++ NP + L M
Sbjct: 423 DSSLEFKGNDFEYLPFGSGRRMCPGMPMASKQVPLVLASLLHFFDWSLPYEQNP-ENLSM 481

Query: 257 EEKFGLALQKEKPLVLIPKLRKM 189
             K+GL L KE+PL+LIPK++ +
Sbjct: 482 RAKYGLTLVKEEPLILIPKIKSI 504


>ref|XP_002318834.2| hypothetical protein POPTR_0012s13620g [Populus trichocarpa]
           gi|550327062|gb|EEE97054.2| hypothetical protein
           POPTR_0012s13620g [Populus trichocarpa]
          Length = 506

 Score =  228 bits (582), Expect = 3e-57
 Identities = 113/202 (55%), Positives = 147/202 (72%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           ELL A +D+S+ST+EWA+AELM++P+AM+++R EL   T + ++ ES L   PYLH  VK
Sbjct: 306 ELLAAVSDSSSSTIEWAMAELMRNPQAMKQLREELAGETPEDLITESSLAKFPYLHLCVK 365

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A+E C+VL+ TIPKDT+V VNVWAI RDP +WEDPL FKPERF 
Sbjct: 366 ETLRLHPPAPLLIPHRATEDCQVLDCTIPKDTQVLVNVWAIARDPASWEDPLCFKPERFL 425

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +D+  N+  ++PFG+GRRIC GLPMA K+V L LA LI  FDW LP+++ P D+LDM
Sbjct: 426 NSDLDYKGNHFEFLPFGSGRRICAGLPMAVKKVQLALANLIHGFDWSLPNNMLP-DELDM 484

Query: 257 EEKFGLALQKEKPLVLIPKLRK 192
            EK+G+ L KE+PL LIPKLRK
Sbjct: 485 AEKYGITLMKEQPLKLIPKLRK 506


>ref|XP_010662049.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 472

 Score =  228 bits (580), Expect = 5e-57
 Identities = 110/202 (54%), Positives = 144/202 (71%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           ELL AG D+S+ TVEWA+AEL++ PE+++++R EL T  +  ++K+SDL  LPYL + +K
Sbjct: 272 ELLVAGTDSSSVTVEWAMAELIRSPESLKKIREELTTEINQNMLKDSDLRKLPYLQACLK 331

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A E+CKV+NYTIPKD +V VN WAIGRDP +WEDPL FKPERF 
Sbjct: 332 ETLRLHPPGPFLLPHRAVESCKVMNYTIPKDAQVLVNAWAIGRDPMSWEDPLVFKPERFL 391

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +DF  NN  +IPF + RRICPGLPMA K +PL+LA  I  FDW LP+  +P D +DM
Sbjct: 392 NSTVDFQGNNFEFIPFSSRRRICPGLPMAVKLIPLVLASWIHFFDWSLPNGGDPKD-IDM 450

Query: 257 EEKFGLALQKEKPLVLIPKLRK 192
            EK+   ++KE+PL+LIPK RK
Sbjct: 451 SEKYSANIRKEQPLLLIPKGRK 472


>emb|CBI34743.3| unnamed protein product [Vitis vinifera]
          Length = 569

 Score =  228 bits (580), Expect = 5e-57
 Identities = 110/202 (54%), Positives = 144/202 (71%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           ELL AG D+S+ TVEWA+AEL++ PE+++++R EL T  +  ++K+SDL  LPYL + +K
Sbjct: 369 ELLVAGTDSSSVTVEWAMAELIRSPESLKKIREELTTEINQNMLKDSDLRKLPYLQACLK 428

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A E+CKV+NYTIPKD +V VN WAIGRDP +WEDPL FKPERF 
Sbjct: 429 ETLRLHPPGPFLLPHRAVESCKVMNYTIPKDAQVLVNAWAIGRDPMSWEDPLVFKPERFL 488

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +DF  NN  +IPF + RRICPGLPMA K +PL+LA  I  FDW LP+  +P D +DM
Sbjct: 489 NSTVDFQGNNFEFIPFSSRRRICPGLPMAVKLIPLVLASWIHFFDWSLPNGGDPKD-IDM 547

Query: 257 EEKFGLALQKEKPLVLIPKLRK 192
            EK+   ++KE+PL+LIPK RK
Sbjct: 548 SEKYSANIRKEQPLLLIPKGRK 569



 Score = 68.2 bits (165), Expect = 7e-09
 Identities = 29/60 (48%), Positives = 44/60 (73%)
 Frame = -2

Query: 413 NNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDMEEKFGLAL 234
           +N  +IP+ +GRRICPGLPMA K +PL+LA  I  F+W LP+  +PT  +D  +K+G+++
Sbjct: 73  HNFEFIPYSSGRRICPGLPMAVKLIPLVLASWIHFFEWSLPNGGDPT--IDTSDKYGVSV 130


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
           AltName: Full=Cytochrome P450 80B1
           gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 487

 Score =  227 bits (579), Expect = 6e-57
 Identities = 107/201 (53%), Positives = 140/201 (69%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           EL  AG + S ST+EWA+ EL K+P+   ++R E++TV  ++ VKESD P+LPYL + VK
Sbjct: 287 ELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVK 346

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A ETC +LNYTIPKD ++ VN W IGRDPK W DPLTF PERF 
Sbjct: 347 ETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFL 406

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +DF  N+ + IPFGAGRRICPGLP+A + + L++A  +Q+ DWCLP+ ++  D L +
Sbjct: 407 NSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMS-VDHLIV 465

Query: 257 EEKFGLALQKEKPLVLIPKLR 195
           EEKFGL LQKE PL ++PK R
Sbjct: 466 EEKFGLTLQKEPPLFIVPKSR 486


>ref|XP_002509713.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
           communis] gi|223549612|gb|EEF51100.1|
           (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
           [Ricinus communis]
          Length = 501

 Score =  227 bits (578), Expect = 8e-57
 Identities = 111/204 (54%), Positives = 147/204 (72%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           ELL AG D S++T+EW +AELMK+P+ M++V+ E+       ++KES + +L YL + VK
Sbjct: 294 ELLSAGTDTSSNTIEWTMAELMKNPKCMKKVQEEITRNLIPDILKESPISNLTYLQACVK 353

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A++TC+V+NYTIPK+++V VN WAIGRDPK W+DPL FKPERF 
Sbjct: 354 ETLRLHPPGPFLLPHRATDTCQVMNYTIPKNSQVLVNFWAIGRDPKYWKDPLIFKPERFL 413

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +D+  N+  +IPFG+GRRICPGLPMAAKQVPLI+A LI  FDW LP   +  D LDM
Sbjct: 414 NSNLDYKGNDFEFIPFGSGRRICPGLPMAAKQVPLIVASLIHFFDWSLPGGKDSID-LDM 472

Query: 257 EEKFGLALQKEKPLVLIPKLRKMN 186
            EK+GL L+ EKPL+LIPK++  N
Sbjct: 473 TEKYGLTLRMEKPLLLIPKIKLRN 496


>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
           californica [Expression vector pWCD2353]
          Length = 490

 Score =  226 bits (577), Expect = 1e-56
 Identities = 108/201 (53%), Positives = 140/201 (69%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           EL  AG + S ST+EWA+ EL K+P+   ++R EL+TV  ++ VKESD P+LPYL + VK
Sbjct: 288 ELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVK 347

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A ETC +LNYTIPKD ++ VN W IGRDPK W DPLTF PERF 
Sbjct: 348 ETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFL 407

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +DF  N+ + IPFGAGRRICPGLP+A + + L++A  +Q+ DWCLP+ ++  D L +
Sbjct: 408 NSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMS-VDHLIV 466

Query: 257 EEKFGLALQKEKPLVLIPKLR 195
           EEKFGL LQKE PL ++PK R
Sbjct: 467 EEKFGLTLQKEPPLFIVPKSR 487


>ref|XP_011030061.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Populus euphratica]
          Length = 511

 Score =  226 bits (577), Expect = 1e-56
 Identities = 106/201 (52%), Positives = 144/201 (71%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           EL  AG D + +T+EWA+AE++K+ E M++V  ELE   +   + ESD+  LPYL + +K
Sbjct: 311 ELFSAGTDTTATTIEWAVAEILKNKEVMKKVDEELEREITKNTISESDISQLPYLDACIK 370

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A+ETC+V+ YT+PKD++V VNVWAI RDP  W+DPL+FKP+RF 
Sbjct: 371 ETLRLHPPVPLLVPHRAAETCEVMKYTVPKDSQVLVNVWAISRDPSTWDDPLSFKPDRFL 430

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
            S +DF   N+ ++PFGAGRRICPGLP+A K VPLILA LI+ FDW LP+  +P  +LDM
Sbjct: 431 GSNLDFKGGNYEFLPFGAGRRICPGLPLANKLVPLILASLIRCFDWSLPNGEDPA-KLDM 489

Query: 257 EEKFGLALQKEKPLVLIPKLR 195
           ++KFG+ LQKE+PL+L+PK R
Sbjct: 490 KDKFGVVLQKEQPLLLVPKRR 510


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
           AltName: Full=Cytochrome P450 80B2
           gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 488

 Score =  226 bits (577), Expect = 1e-56
 Identities = 108/201 (53%), Positives = 140/201 (69%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           EL  AG + S ST+EWA+ EL K+P+   ++R EL+TV  ++ VKESD P+LPYL + VK
Sbjct: 288 ELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVK 347

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A ETC +LNYTIPKD ++ VN W IGRDPK W DPLTF PERF 
Sbjct: 348 ETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFL 407

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           +S +DF  N+ + IPFGAGRRICPGLP+A + + L++A  +Q+ DWCLP+ ++  D L +
Sbjct: 408 NSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMS-VDHLIV 466

Query: 257 EEKFGLALQKEKPLVLIPKLR 195
           EEKFGL LQKE PL ++PK R
Sbjct: 467 EEKFGLTLQKEPPLFIVPKSR 487


>ref|XP_002274586.2| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
           [Vitis vinifera]
          Length = 498

 Score =  226 bits (577), Expect = 1e-56
 Identities = 106/202 (52%), Positives = 148/202 (73%)
 Frame = -2

Query: 797 ELLFAGADASTSTVEWALAELMKDPEAMRRVRFELETVTSDKVVKESDLPHLPYLHSVVK 618
           EL FAG + S+ TVEWA+AEL+K+ +AM ++  EL  +  +  V+ES LPHLPYL + VK
Sbjct: 297 ELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQIIGESPVRESHLPHLPYLQACVK 356

Query: 617 ETXXXXXXXXXXXXXXASETCKVLNYTIPKDTEVFVNVWAIGRDPKAWEDPLTFKPERFH 438
           ET              A+ETC+++ YTIPKD+ +FVN+WA+GRDP  WEDPL+FKPERF 
Sbjct: 357 ETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWEDPLSFKPERFL 416

Query: 437 DSCMDFNSNNHAYIPFGAGRRICPGLPMAAKQVPLILAGLIQSFDWCLPHDVNPTDQLDM 258
           DS ++F  N+  YIPFGAGRR+CPG+P+AA+ VP+ILA  ++ FDW  P D++   ++DM
Sbjct: 417 DSKLEFKGNDFEYIPFGAGRRMCPGMPLAARLVPMILATFVRLFDWSTPGDMD-FAEIDM 475

Query: 257 EEKFGLALQKEKPLVLIPKLRK 192
           EE+F + L+KE+PL L+P++RK
Sbjct: 476 EERFVITLRKEQPLRLVPRIRK 497