BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum21_contig00022175
         (691 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   311   6e-83
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   193   2e-47
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   193   2e-47
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   185   6e-45
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   185   8e-45

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  311 bits (798), Expect = 6e-83
 Identities = 156/216 (72%), Positives = 176/216 (81%), Gaps = 3/216 (1%)
 Frame = -2

Query: 639 RPKHKNLPPGPHAWPLIGNLPLLRTGT---LHITLTDLARTHGPIMTLWLGAQPTVVAST 469
           +PK+KNLPPGPH WPLIGNLP+L T T   LHITL ++ARTHGPIM LWLG QPTV+AST
Sbjct: 22  KPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMAST 81

Query: 468 PESAMEILKTHDRVCSGRHIRTSFRLKQYIEYSLAWSDRTDYWKRLRKLARTEIFSAKML 289
            E+AMEILKTHDR+ S RHIR SFRLK +I+YSL WSD TDYWK LRK+ RTEIFS KML
Sbjct: 82  AEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKML 141

Query: 288 QAQRRVREEKIGELMDYLRSKEGQAVKISKFAFGTLLNVLGNAVFSKNVFVYXXXXXXXX 109
           QAQ  VRE+K+ EL+D+LRSKEGQ VKIS+F FGTLLN+LGN VFSK+VFVY        
Sbjct: 142 QAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVY---GDETD 198

Query: 108 XXDIQNLTREMLTIGAEPNLADFYPILEELDLQGQK 1
              IQNL REML IGAEPN+A+FYP LEELDLQG K
Sbjct: 199 KGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLK 234


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
           AltName: Full=Cytochrome P450 80B1
           gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 487

 Score =  193 bits (491), Expect = 2e-47
 Identities = 97/211 (45%), Positives = 140/211 (66%), Gaps = 1/211 (0%)
 Frame = -2

Query: 630 HKNLPPGPHAWPLIGNLPLLRTGTL-HITLTDLARTHGPIMTLWLGAQPTVVASTPESAM 454
           HKNLPPGP  WP++GNL  L+ G   H    +LA+T+G I TL +G +  VVAST  +A 
Sbjct: 25  HKNLPPGPKPWPIVGNL--LQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAAS 82

Query: 453 EILKTHDRVCSGRHIRTSFRLKQYIEYSLAWSDRTDYWKRLRKLARTEIFSAKMLQAQRR 274
           EILKTHDR+ S R++  SFR+K ++E S+ WSD T+ WK LRK+ RTE+F+ KM+++Q  
Sbjct: 83  EILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAH 142

Query: 273 VREEKIGELMDYLRSKEGQAVKISKFAFGTLLNVLGNAVFSKNVFVYXXXXXXXXXXDIQ 94
           VRE+K  E+++YL  K+G+ VKI +  FGTL+N+ GN +FS+N+F            + +
Sbjct: 143 VREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIF--ELGDPNSGSSEFK 200

Query: 93  NLTREMLTIGAEPNLADFYPILEELDLQGQK 1
                ML +G   N AD++P+L + DL GQ+
Sbjct: 201 EYLWRMLELGNSTNPADYFPMLGKFDLFGQR 231


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
           AltName: Full=Cytochrome P450 80B2
           gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
           3'-hydroxylase [Eschscholzia californica]
          Length = 488

 Score =  193 bits (491), Expect = 2e-47
 Identities = 98/211 (46%), Positives = 141/211 (66%), Gaps = 1/211 (0%)
 Frame = -2

Query: 630 HKNLPPGPHAWPLIGNLPLLRTGTL-HITLTDLARTHGPIMTLWLGAQPTVVASTPESAM 454
           HKNLPPGP  WP++GNL  L+ G   H    +LA+T+G I TL +G +  VVAST  +A 
Sbjct: 26  HKNLPPGPKPWPIVGNL--LQLGEKPHAQFAELAQTYGDIFTLKMGTETVVVASTSSAAS 83

Query: 453 EILKTHDRVCSGRHIRTSFRLKQYIEYSLAWSDRTDYWKRLRKLARTEIFSAKMLQAQRR 274
           EILKTHDR+ S R++  SFR+K ++E S+ WSD T+ WK LRK+ RTE+F+ KM+++Q  
Sbjct: 84  EILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAH 143

Query: 273 VREEKIGELMDYLRSKEGQAVKISKFAFGTLLNVLGNAVFSKNVFVYXXXXXXXXXXDIQ 94
           VRE+K  E+++YL  K+G+ VKI +  FGTL+N+ GN +FS+N+F            + +
Sbjct: 144 VREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIF--ELGXPNSGSSEFK 201

Query: 93  NLTREMLTIGAEPNLADFYPILEELDLQGQK 1
                ML +G   N AD++P+L + DL GQ+
Sbjct: 202 EYLWRMLELGNSTNPADYFPMLGKFDLFGQR 232


>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  185 bits (470), Expect = 6e-45
 Identities = 96/209 (45%), Positives = 138/209 (66%), Gaps = 1/209 (0%)
 Frame = -2

Query: 627 KNLPPGPHAWPLIGNLPLLRTGTL-HITLTDLARTHGPIMTLWLGAQPTVVASTPESAME 451
           K LPPGP  WP++GNL  L+ G   H     LA T+G + +L LG++  VVASTP +A E
Sbjct: 21  KGLPPGPKPWPIVGNL--LQLGEKPHSQFAQLAETYGDLFSLKLGSETVVVASTPLAASE 78

Query: 450 ILKTHDRVCSGRHIRTSFRLKQYIEYSLAWSDRTDYWKRLRKLARTEIFSAKMLQAQRRV 271
           ILKTHDRV SGR++  SFR+K+++E S+ WS+  + WK+LRK+ RTE+F+ KM+++Q  V
Sbjct: 79  ILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEV 138

Query: 270 REEKIGELMDYLRSKEGQAVKISKFAFGTLLNVLGNAVFSKNVFVYXXXXXXXXXXDIQN 91
           RE K  E+++YL+   G  VKI++  FGTL+N+ GN +FS+N+F              ++
Sbjct: 139 RESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMK-EH 197

Query: 90  LTREMLTIGAEPNLADFYPILEELDLQGQ 4
           L R ML +G   N AD++P L + DL GQ
Sbjct: 198 LWR-MLELGNSTNPADYFPFLGKFDLFGQ 225


>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  185 bits (469), Expect = 8e-45
 Identities = 94/210 (44%), Positives = 139/210 (66%), Gaps = 1/210 (0%)
 Frame = -2

Query: 627 KNLPPGPHAWPLIGNLPLLRTGTL-HITLTDLARTHGPIMTLWLGAQPTVVASTPESAME 451
           K LPPGP  WP++GNL  L+ G   H    +LA+T+G + TL LG++  VVASTP +A E
Sbjct: 26  KGLPPGPKPWPIVGNL--LQLGEKPHSQFAELAKTYGDLFTLKLGSETVVVASTPLAASE 83

Query: 450 ILKTHDRVCSGRHIRTSFRLKQYIEYSLAWSDRTDYWKRLRKLARTEIFSAKMLQAQRRV 271
           ILKTHDRV SGR++  SFR+K+++E S+ WS+  + WK+LRK+ R E+F+ KM+++Q  +
Sbjct: 84  ILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKMIESQAEI 143

Query: 270 REEKIGELMDYLRSKEGQAVKISKFAFGTLLNVLGNAVFSKNVFVYXXXXXXXXXXDIQN 91
           RE K  E++++L+  +G  VKI +  FGTL+N+ GN +FS+N+F              ++
Sbjct: 144 RESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMK-EH 202

Query: 90  LTREMLTIGAEPNLADFYPILEELDLQGQK 1
           L R ML +G   N AD++P L   DL GQ+
Sbjct: 203 LWR-MLELGNSTNPADYFPFLGRFDLFGQR 231