BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum21_contig00012475
         (1436 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283285.2| PREDICTED: uncharacterized protein LOC100252...   408   e-136
emb|CBI19420.3| unnamed protein product [Vitis vinifera]              408   e-131
ref|NP_001190458.1| TBP-associated factor 4 [Arabidopsis thalian...   343   e-104
ref|NP_199127.3| TBP-associated factor 4 [Arabidopsis thaliana] ...   343   e-104
ref|XP_002510115.1| transcription initiation factor, putative [R...   384   e-104

>ref|XP_002283285.2| PREDICTED: uncharacterized protein LOC100252311 [Vitis vinifera]
          Length = 922

 Score =  408 bits (1048), Expect(2) = e-136
 Identities = 207/287 (72%), Positives = 243/287 (84%), Gaps = 3/287 (1%)
 Frame = -3

Query: 855  GANTKTPPKKPSIGQKKPLDTLGTPPSLASKKQKVSGGFLDQSIEQLNDVTTVSGVNLRE 676
            G NT+TPPKKPSIGQKKPL+ LG+ P L SKKQKVSG FLDQSIEQLNDVT VSGVNLRE
Sbjct: 570  GINTRTPPKKPSIGQKKPLEALGSSPPLPSKKQKVSGAFLDQSIEQLNDVTAVSGVNLRE 629

Query: 675  EEEQLFSGPKEDSRASEATRRVVQEEEERLILQRIPFQKKMAKIMSKCGIKNVSKDVERC 496
            EEEQLFSGPKEDSR SEA+RRVVQEEEERLILQ+ P QKK+A+IM++C +KN+S DVERC
Sbjct: 630  EEEQLFSGPKEDSRVSEASRRVVQEEEERLILQKAPLQKKLAEIMARCSLKNISNDVERC 689

Query: 495  LSLCLEERMRGMINNLIRVSKQRVDIERSRHRTIITSDVRRQILVMNRMAKEEWEKKQAV 316
            LSLC+EER+RG I+NLIR+SKQR D+E+ RHR+IITSD+R+QIL+MN  A+EEWEKKQA 
Sbjct: 690  LSLCVEERLRGFISNLIRLSKQRADVEKPRHRSIITSDIRQQILIMNHKAREEWEKKQA- 748

Query: 315  EAEKLRKVNEADGNTGVDG--EKDENRTKALKGNKDGDDKMRTXXXXXXXXXXVGGDDML 142
            EAEKLRK+NE +G+TGVDG  +KDE R K+LK NK+ DDKMRT          VGGDDML
Sbjct: 749  EAEKLRKLNEPEGSTGVDGDKDKDEGRVKSLKANKEEDDKMRTTAANVAARAAVGGDDML 808

Query: 141  SKWQLMAEQSRQKRE-GVESTSSVQMGKDVTRKPSSTTGRTSRETQD 4
            SKWQLMAEQ+RQKRE G+++ S  Q GKD +RK SST+GR +RE Q+
Sbjct: 809  SKWQLMAEQARQKREGGIDAASGSQPGKDASRKLSSTSGRNARENQE 855



 Score =  105 bits (262), Expect(2) = e-136
 Identities = 68/193 (35%), Positives = 94/193 (48%), Gaps = 37/193 (19%)
 Frame = -2

Query: 1435 HPYSGQSVGASSTSLRPQTQDSQIRQVPLPQGLAATQPGGTTHPMSSMGVHNYEMQ---N 1265
            H Y+G +V  S+TS + Q  DSQ+RQVPL Q + +TQ GGT+  M+ M V  +E Q   N
Sbjct: 377  HSYTGTNVNTSATSTKQQPHDSQMRQVPLHQNIGSTQMGGTSQAMNPMSVPKFERQSSVN 436

Query: 1264 EPKRLQGGSFSHLTTQSTLQHNPTSWQSSAGKDKKGSSLASIPYIKQEVPSQTQEQH--- 1094
            +PKR+QGGS  H +  STLQ +   WQSS  K++    ++S+ Y+KQE   QT EQ    
Sbjct: 437  DPKRVQGGSLPHPSNSSTLQQSSVPWQSSTNKEQ----ISSMAYVKQEPADQTNEQQQKS 492

Query: 1093 -------------------------------PYQIXXXXXXXXXXSTPPGTNIISASMPT 1007
                                             Q             PP  N +S+SM T
Sbjct: 493  QLSTPQSLSSFPAVQVEKGNAIPGILKDESLEKQASRIGFSSSMSMLPP--NSVSSSMGT 550

Query: 1006 QLDSSVTLRTQVP 968
             LD +VTL +++P
Sbjct: 551  HLDPNVTLGSRIP 563


>emb|CBI19420.3| unnamed protein product [Vitis vinifera]
          Length = 882

 Score =  408 bits (1048), Expect(2) = e-131
 Identities = 207/287 (72%), Positives = 243/287 (84%), Gaps = 3/287 (1%)
 Frame = -3

Query: 855  GANTKTPPKKPSIGQKKPLDTLGTPPSLASKKQKVSGGFLDQSIEQLNDVTTVSGVNLRE 676
            G NT+TPPKKPSIGQKKPL+ LG+ P L SKKQKVSG FLDQSIEQLNDVT VSGVNLRE
Sbjct: 527  GINTRTPPKKPSIGQKKPLEALGSSPPLPSKKQKVSGAFLDQSIEQLNDVTAVSGVNLRE 586

Query: 675  EEEQLFSGPKEDSRASEATRRVVQEEEERLILQRIPFQKKMAKIMSKCGIKNVSKDVERC 496
            EEEQLFSGPKEDSR SEA+RRVVQEEEERLILQ+ P QKK+A+IM++C +KN+S DVERC
Sbjct: 587  EEEQLFSGPKEDSRVSEASRRVVQEEEERLILQKAPLQKKLAEIMARCSLKNISNDVERC 646

Query: 495  LSLCLEERMRGMINNLIRVSKQRVDIERSRHRTIITSDVRRQILVMNRMAKEEWEKKQAV 316
            LSLC+EER+RG I+NLIR+SKQR D+E+ RHR+IITSD+R+QIL+MN  A+EEWEKKQA 
Sbjct: 647  LSLCVEERLRGFISNLIRLSKQRADVEKPRHRSIITSDIRQQILIMNHKAREEWEKKQA- 705

Query: 315  EAEKLRKVNEADGNTGVDG--EKDENRTKALKGNKDGDDKMRTXXXXXXXXXXVGGDDML 142
            EAEKLRK+NE +G+TGVDG  +KDE R K+LK NK+ DDKMRT          VGGDDML
Sbjct: 706  EAEKLRKLNEPEGSTGVDGDKDKDEGRVKSLKANKEEDDKMRTTAANVAARAAVGGDDML 765

Query: 141  SKWQLMAEQSRQKRE-GVESTSSVQMGKDVTRKPSSTTGRTSRETQD 4
            SKWQLMAEQ+RQKRE G+++ S  Q GKD +RK SST+GR +RE Q+
Sbjct: 766  SKWQLMAEQARQKREGGIDAASGSQPGKDASRKLSSTSGRNARENQE 812



 Score = 89.4 bits (220), Expect(2) = e-131
 Identities = 59/159 (37%), Positives = 82/159 (51%), Gaps = 3/159 (1%)
 Frame = -2

Query: 1435 HPYSGQSVGASSTSLRPQTQDSQIRQVPLPQGLAATQPGGTTHPMSSMGVHNYEMQ---N 1265
            H Y+G +V  S+TS + Q  DSQ+RQVPL Q + +TQ GGT+  M+ M V  +E Q   N
Sbjct: 383  HSYTGTNVNTSATSTKQQPHDSQMRQVPLHQNIGSTQMGGTSQAMNPMSVPKFERQSSVN 442

Query: 1264 EPKRLQGGSFSHLTTQSTLQHNPTSWQSSAGKDKKGSSLASIPYIKQEVPSQTQEQHPYQ 1085
            +PKR+QGGS  H +  STLQ      QSS  +             K ++ +   E    Q
Sbjct: 443  DPKRVQGGSLPHPSNSSTLQ------QSSQQQ-------------KSQLSTPQNESLEKQ 483

Query: 1084 IXXXXXXXXXXSTPPGTNIISASMPTQLDSSVTLRTQVP 968
                         PP  N +S+SM T LD +VTL +++P
Sbjct: 484  ASRIGFSSSMSMLPP--NSVSSSMGTHLDPNVTLGSRIP 520


>ref|NP_001190458.1| TBP-associated factor 4 [Arabidopsis thaliana]
            gi|332007531|gb|AED94914.1| TBP-associated factor 4
            [Arabidopsis thaliana]
          Length = 852

 Score =  343 bits (880), Expect(2) = e-104
 Identities = 187/302 (61%), Positives = 228/302 (75%), Gaps = 9/302 (2%)
 Frame = -3

Query: 879  STTASLGP------GANTKTPPKKPSIGQKKPLDTLGTPPSLASKKQKVSGGFLDQSIEQ 718
            +T  S GP      GAN + PPKKPS+GQKKPL+TLG+ P   SKKQKV+G  +DQSIEQ
Sbjct: 477  TTMNSRGPLGTSQGGANARMPPKKPSVGQKKPLETLGSSPPPPSKKQKVAGNSMDQSIEQ 536

Query: 717  LNDVTTVSGVNLREEEEQLFSGPKEDSRASEATRRVVQEEEERLILQRIPFQKKMAKIMS 538
            LNDVT VSGVNLREEEEQLFSG KED R SEA+RRVV EEEERLILQ+ P Q+K+A+IM+
Sbjct: 537  LNDVTAVSGVNLREEEEQLFSGAKEDGRVSEASRRVVHEEEERLILQKNPLQRKLAEIMA 596

Query: 537  KCGIKNVSKDVERCLSLCLEERMRGMINNLIRVSKQRVDIERSRHRTIITSDVRRQILVM 358
            K G+K +S DVERCLSLC+EERMRG+++++IR+SKQRVD E+SRHRT ITSD+R QI  M
Sbjct: 597  KAGLKQISNDVERCLSLCVEERMRGLLSHIIRLSKQRVDAEKSRHRTFITSDIRLQINEM 656

Query: 357  NRMAKEEWEKKQAVEAEKLRKVNEA-DGNTGVDGEKD--ENRTKALKGNKDGDDKMRTXX 187
            N+  KEEWEKKQA EAEKL+K +E+ +G+ GVD EKD  +NR+K +KGNK+ DDKMRT  
Sbjct: 657  NQKVKEEWEKKQA-EAEKLKKPSESEEGDGGVDSEKDKEDNRSKGVKGNKEDDDKMRTTA 715

Query: 186  XXXXXXXXVGGDDMLSKWQLMAEQSRQKREGVESTSSVQMGKDVTRKPSSTTGRTSRETQ 7
                    VGGDD   KWQLMAE +RQK       S  + GKD  +K +S  G+ S++ Q
Sbjct: 716  ANVAARAAVGGDDAFLKWQLMAE-ARQK-------SVSEAGKDGNQKTTSGGGKNSKDRQ 767

Query: 6    DG 1
            DG
Sbjct: 768  DG 769



 Score = 64.3 bits (155), Expect(2) = e-104
 Identities = 47/160 (29%), Positives = 73/160 (45%), Gaps = 4/160 (2%)
 Frame = -2

Query: 1435 HPYSGQSVGASSTSLRPQTQDSQIRQVPLPQGLAATQPGG----TTHPMSSMGVHNYEMQ 1268
            H + G +  AS ++LRP   DS +R V   Q + +T  GG    TT+ M+          
Sbjct: 327  HSFPGPNTNASGSTLRPHLHDSHMRHVAHNQPMGSTGLGGPPQSTTNMMTMPKFERPSSV 386

Query: 1267 NEPKRLQGGSFSHLTTQSTLQHNPTSWQSSAGKDKKGSSLASIPYIKQEVPSQTQEQHPY 1088
            N+P R+QGG+ SH    S+L  N    Q S+    K  S+    + K    S T  +   
Sbjct: 387  NDPSRVQGGATSHFQNSSSLPLNSAPGQGSSVSHVKQESVDQ-SFEKNNAASMTSNEDLE 445

Query: 1087 QIXXXXXXXXXXSTPPGTNIISASMPTQLDSSVTLRTQVP 968
            +           +  P ++ +S SM TQLD+S T+ ++ P
Sbjct: 446  KESSRMVLSTPNNMAPASS-VSPSMTTQLDASTTMNSRGP 484


>ref|NP_199127.3| TBP-associated factor 4 [Arabidopsis thaliana]
            gi|38490053|gb|AAR21620.1| TATA-binding protein
            associated factor 4b [Arabidopsis thaliana]
            gi|110738778|dbj|BAF01312.1| hypothetical protein
            [Arabidopsis thaliana] gi|332007530|gb|AED94913.1|
            TBP-associated factor 4 [Arabidopsis thaliana]
          Length = 823

 Score =  343 bits (880), Expect(2) = e-104
 Identities = 187/302 (61%), Positives = 228/302 (75%), Gaps = 9/302 (2%)
 Frame = -3

Query: 879  STTASLGP------GANTKTPPKKPSIGQKKPLDTLGTPPSLASKKQKVSGGFLDQSIEQ 718
            +T  S GP      GAN + PPKKPS+GQKKPL+TLG+ P   SKKQKV+G  +DQSIEQ
Sbjct: 477  TTMNSRGPLGTSQGGANARMPPKKPSVGQKKPLETLGSSPPPPSKKQKVAGNSMDQSIEQ 536

Query: 717  LNDVTTVSGVNLREEEEQLFSGPKEDSRASEATRRVVQEEEERLILQRIPFQKKMAKIMS 538
            LNDVT VSGVNLREEEEQLFSG KED R SEA+RRVV EEEERLILQ+ P Q+K+A+IM+
Sbjct: 537  LNDVTAVSGVNLREEEEQLFSGAKEDGRVSEASRRVVHEEEERLILQKNPLQRKLAEIMA 596

Query: 537  KCGIKNVSKDVERCLSLCLEERMRGMINNLIRVSKQRVDIERSRHRTIITSDVRRQILVM 358
            K G+K +S DVERCLSLC+EERMRG+++++IR+SKQRVD E+SRHRT ITSD+R QI  M
Sbjct: 597  KAGLKQISNDVERCLSLCVEERMRGLLSHIIRLSKQRVDAEKSRHRTFITSDIRLQINEM 656

Query: 357  NRMAKEEWEKKQAVEAEKLRKVNEA-DGNTGVDGEKD--ENRTKALKGNKDGDDKMRTXX 187
            N+  KEEWEKKQA EAEKL+K +E+ +G+ GVD EKD  +NR+K +KGNK+ DDKMRT  
Sbjct: 657  NQKVKEEWEKKQA-EAEKLKKPSESEEGDGGVDSEKDKEDNRSKGVKGNKEDDDKMRTTA 715

Query: 186  XXXXXXXXVGGDDMLSKWQLMAEQSRQKREGVESTSSVQMGKDVTRKPSSTTGRTSRETQ 7
                    VGGDD   KWQLMAE +RQK       S  + GKD  +K +S  G+ S++ Q
Sbjct: 716  ANVAARAAVGGDDAFLKWQLMAE-ARQK-------SVSEAGKDGNQKTTSGGGKNSKDRQ 767

Query: 6    DG 1
            DG
Sbjct: 768  DG 769



 Score = 64.3 bits (155), Expect(2) = e-104
 Identities = 47/160 (29%), Positives = 73/160 (45%), Gaps = 4/160 (2%)
 Frame = -2

Query: 1435 HPYSGQSVGASSTSLRPQTQDSQIRQVPLPQGLAATQPGG----TTHPMSSMGVHNYEMQ 1268
            H + G +  AS ++LRP   DS +R V   Q + +T  GG    TT+ M+          
Sbjct: 327  HSFPGPNTNASGSTLRPHLHDSHMRHVAHNQPMGSTGLGGPPQSTTNMMTMPKFERPSSV 386

Query: 1267 NEPKRLQGGSFSHLTTQSTLQHNPTSWQSSAGKDKKGSSLASIPYIKQEVPSQTQEQHPY 1088
            N+P R+QGG+ SH    S+L  N    Q S+    K  S+    + K    S T  +   
Sbjct: 387  NDPSRVQGGATSHFQNSSSLPLNSAPGQGSSVSHVKQESVDQ-SFEKNNAASMTSNEDLE 445

Query: 1087 QIXXXXXXXXXXSTPPGTNIISASMPTQLDSSVTLRTQVP 968
            +           +  P ++ +S SM TQLD+S T+ ++ P
Sbjct: 446  KESSRMVLSTPNNMAPASS-VSPSMTTQLDASTTMNSRGP 484


>ref|XP_002510115.1| transcription initiation factor, putative [Ricinus communis]
            gi|223550816|gb|EEF52302.1| transcription initiation
            factor, putative [Ricinus communis]
          Length = 925

 Score =  384 bits (987), Expect = e-104
 Identities = 199/291 (68%), Positives = 238/291 (81%), Gaps = 7/291 (2%)
 Frame = -3

Query: 855  GANTKTPPKKPSIGQKKPLDTLGTPPSLASKKQKVSGGFLDQSIEQLNDVTTVSGVNLRE 676
            G N +TP KK SIGQKKPL+ LG+ P ++SKKQKVSG FLDQSIEQLNDVT VSGVNLRE
Sbjct: 569  GVNARTPTKKLSIGQKKPLEALGSSPPMSSKKQKVSGAFLDQSIEQLNDVTAVSGVNLRE 628

Query: 675  EEEQLFSGPKEDSRASEATRRVVQEEEERLILQRIPFQKKMAKIMSKCGIKNVSKDVERC 496
            EEEQLFSG KEDSR SEA+RRVVQEEEERLILQ+ P QKK+A+IM KCG+KN++ DVERC
Sbjct: 629  EEEQLFSGSKEDSRVSEASRRVVQEEEERLILQKTPLQKKLAEIMVKCGLKNINNDVERC 688

Query: 495  LSLCLEERMRGMINNLIRVSKQRVDIERSRHRTIITSDVRRQILVMNRMAKEEWEKKQAV 316
            LSLC+EERMRG+I+ LIR+SKQRVD E+SRHRT+ITSDVR+QI+ MN+ A+EEWE+KQA 
Sbjct: 689  LSLCVEERMRGLISTLIRLSKQRVDAEKSRHRTVITSDVRQQIMTMNQKAREEWERKQA- 747

Query: 315  EAEKLRKVNEADGNTGVDG--EKDENRTKALKG----NKDGDDKMRTXXXXXXXXXXVGG 154
            EAEKLRKVNE +G+ GV+G  EKD+ R KA+KG    NK+ DDKMRT          VGG
Sbjct: 748  EAEKLRKVNEPEGDNGVEGDKEKDDGRVKAIKGNIPANKEEDDKMRTTAANVAARAAVGG 807

Query: 153  DDMLSKWQLMAEQSRQKRE-GVESTSSVQMGKDVTRKPSSTTGRTSRETQD 4
            DD LSKWQLMAEQ+RQKRE G+E+ S     K+VTRKP  T+G++ ++ Q+
Sbjct: 808  DDHLSKWQLMAEQARQKREGGIEAASGSYSAKEVTRKPQFTSGKSMKDNQE 858



 Score = 75.1 bits (183), Expect = 4e-11
 Identities = 40/116 (34%), Positives = 62/116 (53%), Gaps = 3/116 (2%)
 Frame = -2

Query: 1435 HPYSGQSVGASSTSLRPQTQDSQIRQVPLPQGLAATQPGGTTHPMSSMGVHNYEMQN--- 1265
            HPYSG ++  S +S++ Q  D Q+RQ+     +A+TQ GG+T  ++ + V  +E  N   
Sbjct: 376  HPYSGTNINTSGSSMKTQPHDLQMRQIS-HSTMASTQIGGSTPTLNMVHVSKFERPNSVS 434

Query: 1264 EPKRLQGGSFSHLTTQSTLQHNPTSWQSSAGKDKKGSSLASIPYIKQEVPSQTQEQ 1097
            +P R+Q GS S    +S L  N   WQ+   K++      S  Y+KQE   Q  +Q
Sbjct: 435  DPSRVQSGSMSQYNNKSALPQNSIPWQAPTNKEQTSPLFPSTNYVKQEPLEQATDQ 490